MC11g_new0169 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g_new0169 vs. ExPASy Swiss-Prot
Match: H2DH21 (Cytochrome P450 CYP72A219 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 4.5e-174
Identity = 277/519 (53.37%), Postives = 394/519 (75.92%), Query Frame = 0

Query: 1   METSVEVVVSIC-MVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELA 60
           ME  ++++ S C +V++ L+GWR+F+W+WLRP++L+ +L  QGF GNSYR+F GD+KE+ 
Sbjct: 1   MELVLKLISSFCAIVVVILLGWRIFNWVWLRPRKLEKYLRNQGFNGNSYRLFFGDVKEMI 60

Query: 61  AASGQARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFT 120
               +A+S P+  +  DI PR++P   K I NYGKNS++WLGP P VHIM+P H+K   +
Sbjct: 61  VMLKEAKSKPINLYD-DIIPRIIPLNQKIITNYGKNSFLWLGPKPMVHIMNPDHIKDVLS 120

Query: 121 QIDEIQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMI 180
           +  + QKP  NP+ KLL  GV   EG +WAKH+K+I+PAF+L KLK+M+P    + +E++
Sbjct: 121 KFYQFQKPRHNPLTKLLATGVADAEGDRWAKHRKLINPAFHLEKLKNMLPAIYLSSSEIV 180

Query: 181 SDWERLISKEGWCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAAS 240
           + WE ++S +G  E+DV+PYL  LT+D ISRTA GSS++EG+ IF+L +E  +L+++A+ 
Sbjct: 181 TKWEEMVSTKGQFELDVLPYLETLTSDVISRTAFGSSYEEGRKIFQLQREQAELIIQASQ 240

Query: 241 RIYIPGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESNL 300
            IY+PG RF+PTK N++MK+I +E+   L  IINKR KAME GE  +  DLLGILLESN 
Sbjct: 241 TIYLPGMRFLPTKRNKRMKEIAKEVKIALKSIINKRLKAMEAGERSSHDDLLGILLESNS 300

Query: 301 REIEEHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEVL 360
           +EI++HGN    G++++EV++EC++F+ AGQETT++LL+WTM+ LS H+ WQ RA+ EVL
Sbjct: 301 KEIKQHGNT-NFGLTVDEVIEECKLFFFAGQETTSNLLVWTMILLSQHQDWQKRAKEEVL 360

Query: 361 QVFGNKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCIP 420
           + FGN  P FDGL+ LKVV MI+ EV+RLYPP  ++ R + +E +LG +++P+GVIL +P
Sbjct: 361 RTFGNNKPDFDGLNHLKVVNMILLEVLRLYPPILSLDRTIYEEIKLGEISLPAGVILLLP 420

Query: 421 VILIHRDREIWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKIV 480
           +IL+H D+EIWGDDA EF PERFSEG+  A+K +  + PF+WGPRIC+GQNFAM+EAK+ 
Sbjct: 421 IILLHYDQEIWGDDAKEFNPERFSEGVLKATKGRVTYFPFSWGPRICIGQNFAMLEAKMA 480

Query: 481 FSIILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL 519
            ++ILQ FSF LS SY HAP +I+T QPQ+GAH+IL  L
Sbjct: 481 MAMILQRFSFVLSPSYAHAPHAIITLQPQYGAHLILHSL 517

BLAST of MC11g_new0169 vs. ExPASy Swiss-Prot
Match: A0A0S2IHL2 (Cytochrome P450 72A397 OS=Kalopanax septemlobus OX=228393 GN=CYP72A397 PE=1 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 7.0e-167
Identity = 275/516 (53.29%), Postives = 381/516 (73.84%), Query Frame = 0

Query: 3   TSVEVVVSICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELAAAS 62
           T +   V++ +V++    W+V +W+W+ P++L+  L +QGF GNSYR+F GDLKE +  +
Sbjct: 9   TKIAAAVAVAVVVVGW-AWKVLNWVWVSPRKLEESLRKQGFRGNSYRLFYGDLKESSEMT 68

Query: 63  GQARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFTQID 122
            +A+  P+   S D   RV P  ++T++ YGK+S++W+GP PRV IMDP  +K    +  
Sbjct: 69  RKAKLKPI-NLSDDPVLRVRPFIHQTVKKYGKSSFIWIGPTPRVQIMDPEIIKEIMVKSY 128

Query: 123 EIQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMISDW 182
           +  KP  NP+VKL  DG+  +EG+ WAKH+K+++PAF+L +LK M+P    +C EM+S W
Sbjct: 129 KFNKPKRNPLVKLFADGLANHEGELWAKHRKLLNPAFHLERLKCMLPAMYFSCIEMVSKW 188

Query: 183 ERLISKEGWCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAASRIY 242
           +++ISK+G  E+DV P+L  LT+D IS TA GSS++EG I+F+L  E  +LV++    +Y
Sbjct: 189 DKMISKDGSRELDVWPFLQRLTSDVISHTAFGSSYEEGNIVFELQTEQAELVMKTLQSVY 248

Query: 243 IPGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESNLREI 302
           IPGW ++PTK NRKMK+IDR+  + LM IINK+ KAM+ GE G+  D+LGILLESNL+E 
Sbjct: 249 IPGWSYLPTKRNRKMKEIDRKTQSCLMNIINKKTKAMQAGE-GSTDDILGILLESNLKEQ 308

Query: 303 EEHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEVLQVF 362
              G  K  GMSI+EV+ EC+ FY AGQETT+ LL+WTM+ LS+H  WQ RAR EVLQ F
Sbjct: 309 LGQG-KKNVGMSIQEVMGECKQFYFAGQETTSGLLVWTMVLLSIHPNWQARAREEVLQQF 368

Query: 363 GNKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCIPVIL 422
           GN  P FD L+ LK+VTMI+ EV+RLYPP +T+ R V +ET LG++T+P+GV + +P+++
Sbjct: 369 GNAKPDFDNLNHLKIVTMILYEVLRLYPPVDTLFRRVDQETTLGDITLPAGVQISLPIMI 428

Query: 423 IHRDREIWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKIVFSI 482
           +H D+ IWGDDA EF PERFSEG+S A+K Q  F PF WGPRIC+GQNFA++EAK+  +I
Sbjct: 429 LHHDQNIWGDDAKEFNPERFSEGVSKATKNQVVFFPFGWGPRICIGQNFALLEAKLALAI 488

Query: 483 ILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL 519
           ILQ FSFELS SYTHAPT+++T QPQHGA++IL KL
Sbjct: 489 ILQRFSFELSPSYTHAPTTVLTVQPQHGANLILHKL 520

BLAST of MC11g_new0169 vs. ExPASy Swiss-Prot
Match: A0A481NR20 (Cytochrome P450 72A552 OS=Barbarea vulgaris OX=50459 GN=CYP72A552 PE=1 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 6.0e-158
Identity = 267/520 (51.35%), Postives = 373/520 (71.73%), Query Frame = 0

Query: 1   METSV-EVVVSICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELA 60
           ME SV  V VS+ + +++   WR   W+W +PK L+++L RQG +G  Y   VGDLK  +
Sbjct: 1   MEISVASVTVSVVIAVVTWWVWRTLKWVWFQPKMLESYLRRQGLSGTPYTPLVGDLKRNS 60

Query: 61  AASGQARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFT 120
               +A S P+   + DI  RVVP   + ++ YG+  + WLGP P + IMDP  +K  F 
Sbjct: 61  KMLTEAISKPI-RLNDDITQRVVPYPLQMLKTYGRTHFTWLGPIPAITIMDPELIKEVFN 120

Query: 121 QIDEIQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMI 180
           ++ + QK    P+ +L+  G++ Y+G KWAKH+KII+PAF+L KLK+MVP F   C+E++
Sbjct: 121 RVYDFQKARLFPLARLIATGLVRYDGDKWAKHRKIINPAFHLEKLKNMVPAFHQCCSEVV 180

Query: 181 SDWERLIS-KEGWCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAA 240
             W++L+S K   CE+DV P L ++TAD ISRTA GSS+KEGQ IF+L +E+ +L++++ 
Sbjct: 181 GAWDKLVSDKRSSCEVDVWPGLVSMTADMISRTAFGSSYKEGQRIFELQEEIKELLIQSL 240

Query: 241 SRIYIPGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESN 300
            + +IPG+ ++PTK NR+MK  DREI  +L GI+NKR +A E GE  ++ DLLGILLESN
Sbjct: 241 GKAFIPGYHYLPTKGNRRMKAADREIKVILRGIVNKRLRAREAGEAPSE-DLLGILLESN 300

Query: 301 LREIEEHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEV 360
           L      G  K +GMSIE+V++EC++FYLAGQETT+ LL+WTM+ LS H+ WQ RAR EV
Sbjct: 301 L------GQAKGNGMSIEDVMEECKLFYLAGQETTSVLLVWTMVMLSQHQDWQARAREEV 360

Query: 361 LQVFGNKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCI 420
            QVFG+K+P  +GL+ LKV+TMI+ EV+RLYPP   + R + KE +LG++T+P+GV + +
Sbjct: 361 KQVFGDKEPNTEGLNQLKVMTMILYEVLRLYPPVTQLPRAIHKEMKLGDMTLPAGVHINL 420

Query: 421 PVILIHRDREIWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKI 480
           P++L+ RD E+WG+DA EFKPERF +G+S A+K Q  F  FAWGPRIC+GQNFA+MEAK+
Sbjct: 421 PIMLVQRDTELWGNDAAEFKPERFKDGLSKAAKNQVSFFSFAWGPRICIGQNFALMEAKM 480

Query: 481 VFSIILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL 519
             ++ILQ FS ELS SY HAP S++T  PQ GAH+IL KL
Sbjct: 481 AMALILQRFSLELSPSYVHAPYSVITLHPQFGAHLILHKL 512

BLAST of MC11g_new0169 vs. ExPASy Swiss-Prot
Match: Q9LUC8 (Cytochrome P450 72A13 OS=Arabidopsis thaliana OX=3702 GN=CYP72A13 PE=2 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 3.0e-157
Identity = 265/520 (50.96%), Postives = 373/520 (71.73%), Query Frame = 0

Query: 1   METSV-EVVVSICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELA 60
           ME SV  V VS+ +V++S   WR    +WL+PK L+++L RQG AG  Y   VGDLK   
Sbjct: 1   MEISVASVTVSVAVVVVSWWVWRTLQRVWLKPKMLESYLRRQGLAGTPYTPLVGDLKRNF 60

Query: 61  AASGQARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFT 120
           +   +ARS P+   + DI PR+VP   + ++ +G+  + W GP P + IMDP  +K  F 
Sbjct: 61  SMLAEARSKPI-NLTDDITPRIVPYPLQMLKTHGRTFFTWFGPIPTITIMDPEQIKEVFN 120

Query: 121 QIDEIQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMI 180
           ++ + QK +  P+ +L+  G+++Y+G KW KH++II+PAF+L K+K+MVP F  +C+E++
Sbjct: 121 KVYDFQKAHTFPLGRLIAAGLVSYDGDKWTKHRRIINPAFHLEKIKNMVPAFHQSCSEIV 180

Query: 181 SDWERLIS-KEGWCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAA 240
            +W++L++ K+  CE+D+ P+L ++TAD ISRTA GSS+KEGQ IF+L  EL  L+++A 
Sbjct: 181 GEWDKLVTDKQSSCEVDIWPWLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAF 240

Query: 241 SRIYIPGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESN 300
            +  IPG+R+ PTK NR+MK   REI  +L GI+NKR +A E GE     DLLGILLESN
Sbjct: 241 RKAIIPGYRYFPTKGNRRMKAAAREIKFILRGIVNKRLRAREAGE-APSDDLLGILLESN 300

Query: 301 LREIEEHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEV 360
           L      G  K +GMS EE+++EC++FY AGQETT  LL+WTM+ LS H+ WQ RAR EV
Sbjct: 301 L------GQTKGNGMSTEELMEECKLFYFAGQETTTVLLVWTMVLLSQHQDWQARAREEV 360

Query: 361 LQVFGNKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCI 420
            QVFG+K+P  +GL+ LKV+TMI+ EV+RLYPP   + R + KE +LG+LT+P GV + +
Sbjct: 361 KQVFGDKEPDAEGLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMQLGDLTLPGGVQISL 420

Query: 421 PVILIHRDREIWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKI 480
           P++LI RDRE+WG+DA EFKP+RF +G+S A+K Q  F PFAWGPRIC+GQNFA++EAK+
Sbjct: 421 PILLIQRDRELWGNDAGEFKPDRFKDGLSKATKNQVSFFPFAWGPRICIGQNFALLEAKM 480

Query: 481 VFSIILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL 519
             ++IL+ FSFELS SY HAP +++TT PQ GA +IL KL
Sbjct: 481 AMTLILRKFSFELSPSYVHAPYTVLTTHPQFGAPLILHKL 512

BLAST of MC11g_new0169 vs. ExPASy Swiss-Prot
Match: Q05047 (Secologanin synthase OS=Catharanthus roseus OX=4058 GN=CYP72A1 PE=1 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 8.6e-157
Identity = 256/521 (49.14%), Postives = 369/521 (70.83%), Query Frame = 0

Query: 1   METSVEVVVSICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELAA 60
           M+T  + + +    L+    WRV DW W  PKR++  L +QGF GN YR  VGD+KE   
Sbjct: 5   MDTIRKAIAATIFALVMAWAWRVLDWAWFTPKRIEKRLRQQGFRGNPYRFLVGDVKESGK 64

Query: 61  ASGQARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFTQ 120
              +A S PM  F++DI PR++P    TI  YG+NS+ W+G  PR+H+M+P  +K   T 
Sbjct: 65  MHQEALSKPM-EFNNDIVPRLMPHINHTINTYGRNSFTWMGRIPRIHVMEPELIKEVLTH 124

Query: 121 IDEIQKPNF---NPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNE 180
             + QK NF   NP+VK LL GV ++EG KW+KH++IISPAF L KLK M+P F    ++
Sbjct: 125 SSKYQK-NFDVHNPLVKFLLTGVGSFEGAKWSKHRRIISPAFTLEKLKSMLPAFAICYHD 184

Query: 181 MISDWERLISKEGWCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEA 240
           M++ WE++  K+G  E+D+ P    LT+D IS+ A GS+++EG  IF+LLKEL DL ++ 
Sbjct: 185 MLTKWEKIAEKQGSHEVDIFPTFDVLTSDVISKVAFGSTYEEGGKIFRLLKELMDLTIDC 244

Query: 241 ASRIYIPGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLES 300
              +YIPGW ++PTK N++MK+I++EI  +L  IINKR KA++ GE G + DLLG+LLES
Sbjct: 245 MRDVYIPGWSYLPTKRNKRMKEINKEITDMLRFIINKRMKALKAGEPG-EDDLLGVLLES 304

Query: 301 NLREIEEHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVE 360
           N++EI++ GN K  GMSI +V++EC++FY AGQETT  LL WT + LS H +WQ+RAR E
Sbjct: 305 NIQEIQKQGNKKDGGMSINDVIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREE 364

Query: 361 VLQVFGNKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILC 420
           VLQ FG   P F+ L+ LK V+MI+ EV+RLYPP   + ++V K+T+LG+ TIP+G  + 
Sbjct: 365 VLQAFGKNKPEFERLNHLKYVSMILYEVLRLYPPVIDLTKIVHKDTKLGSYTIPAGTQVM 424

Query: 421 IPVILIHRDREIWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAK 480
           +P +++HR++ IWG+DA EF P RF +G++NA+K    +LPF+WGPR+C+GQNFA+++AK
Sbjct: 425 LPTVMLHREKSIWGEDAMEFNPMRFVDGVANATKNNVTYLPFSWGPRVCLGQNFALLQAK 484

Query: 481 IVFSIILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL 519
           +  ++ILQ F F+++ SY HAP +I+T QPQ G+H+I +KL
Sbjct: 485 LGLAMILQRFKFDVAPSYVHAPFTILTVQPQFGSHVIYKKL 522

BLAST of MC11g_new0169 vs. NCBI nr
Match: XP_022148570.1 (cytochrome P450 CYP72A219-like [Momordica charantia])

HSP 1 Score: 1055 bits (2729), Expect = 0.0
Identity = 518/518 (100.00%), Postives = 518/518 (100.00%), Query Frame = 0

Query: 1   METSVEVVVSICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELAA 60
           METSVEVVVSICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELAA
Sbjct: 1   METSVEVVVSICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELAA 60

Query: 61  ASGQARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFTQ 120
           ASGQARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFTQ
Sbjct: 61  ASGQARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFTQ 120

Query: 121 IDEIQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMIS 180
           IDEIQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMIS
Sbjct: 121 IDEIQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMIS 180

Query: 181 DWERLISKEGWCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAASR 240
           DWERLISKEGWCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAASR
Sbjct: 181 DWERLISKEGWCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAASR 240

Query: 241 IYIPGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESNLR 300
           IYIPGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESNLR
Sbjct: 241 IYIPGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESNLR 300

Query: 301 EIEEHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEVLQ 360
           EIEEHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEVLQ
Sbjct: 301 EIEEHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEVLQ 360

Query: 361 VFGNKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCIPV 420
           VFGNKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCIPV
Sbjct: 361 VFGNKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCIPV 420

Query: 421 ILIHRDREIWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKIVF 480
           ILIHRDREIWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKIVF
Sbjct: 421 ILIHRDREIWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKIVF 480

Query: 481 SIILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL 518
           SIILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL
Sbjct: 481 SIILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL 518

BLAST of MC11g_new0169 vs. NCBI nr
Match: XP_038878667.1 (cytochrome P450 CYP72A219-like isoform X1 [Benincasa hispida])

HSP 1 Score: 705 bits (1819), Expect = 1.26e-250
Identity = 334/516 (64.73%), Postives = 423/516 (81.98%), Query Frame = 0

Query: 4   SVEVVVSICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELAAASG 63
           S+ +V+ +C+V     GWR  +W+WLRPK+L+ +L +QGFAGNSYR++ GD+ ++AA   
Sbjct: 6   SIWMVIMVCLVGW---GWRFLNWIWLRPKKLEKWLRQQGFAGNSYRLYSGDVNDMAAVME 65

Query: 64  QARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFTQIDE 123
           QA+S PM +FSHDIAPRV+PS + TI  YGKNS+ WLGPYPRV+IMDP  LKA F+   +
Sbjct: 66  QAKSKPM-SFSHDIAPRVIPSIHHTIHKYGKNSFTWLGPYPRVYIMDPEQLKAAFSLYTD 125

Query: 124 IQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMISDWE 183
            QKP  NP+ +LL DG++ +EG KWAKH+KII+PAF+  KLKDMVP    +C+EMIS WE
Sbjct: 126 FQKPTINPLGRLLFDGLINHEGAKWAKHRKIINPAFHFEKLKDMVPTMFESCSEMISKWE 185

Query: 184 RLISKEGWCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAASRIYI 243
           R+ISK+G+CE+DVMP+L NL AD ISRTA GS+++EG+IIF LLKELT+LV++ ++ +YI
Sbjct: 186 RMISKDGFCELDVMPHLQNLAADVISRTAFGSTYEEGKIIFNLLKELTNLVIKTSNTVYI 245

Query: 244 PGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESNLREIE 303
           PGWRF+PTK N +MKKI +E+ TLL+ +INKRQ+AM++GE    SDLLGILLESN  EI+
Sbjct: 246 PGWRFLPTKLNNRMKKISKEMKTLLLNLINKRQRAMKEGE-AIHSDLLGILLESNFNEIQ 305

Query: 304 EHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEVLQVFG 363
           EHGN    GMSI+++V+EC++FYLAGQETTA LL+WTM+ LS + +WQDRAR EVLQVFG
Sbjct: 306 EHGN-VNIGMSIDDLVEECKVFYLAGQETTAILLVWTMVLLSSYTKWQDRARSEVLQVFG 365

Query: 364 NKDPTFDGLSSLKVVTMIMNEVMRLYPPANTI-HRVVQKETRLGNLTIPSGVILCIPVIL 423
              P FDGL+ LKVVTMI+NEV+RLYPPA+    R+V+KETRLGNLT+PSGV+L  P++L
Sbjct: 366 YNKPDFDGLNRLKVVTMILNEVLRLYPPASVFSRRLVKKETRLGNLTLPSGVLLSFPILL 425

Query: 424 IHRDREIWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKIVFSI 483
           IH+D E+WG+DA+EF PERF+EGIS A+K Q GF PF WGPRIC+GQNFAM+EAKI  S+
Sbjct: 426 IHQDPELWGEDANEFNPERFAEGISKATKNQTGFFPFGWGPRICVGQNFAMIEAKIALSM 485

Query: 484 ILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL 518
           ILQHFSFELSSSYTHAP SI+T QPQHGAHIIL KL
Sbjct: 486 ILQHFSFELSSSYTHAPISILTIQPQHGAHIILHKL 515

BLAST of MC11g_new0169 vs. NCBI nr
Match: XP_023536193.1 (cytochrome P450 CYP72A219-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 703 bits (1814), Expect = 9.01e-250
Identity = 327/500 (65.40%), Postives = 411/500 (82.20%), Query Frame = 0

Query: 19  VGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELAAASGQARSNPMPTFSHDIA 78
           V W V +W+W+RP++L+ +L +QG AGN YRI  GDLKE AA   +A + P+  FSHDI 
Sbjct: 21  VSWGVLNWVWIRPRKLEKWLKKQGLAGNPYRILHGDLKERAALLEEANAKPV-AFSHDIG 80

Query: 79  PRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFTQIDEIQKPNFNPIVKLLLD 138
            RV+PS YKTIQNYGK SYMWLGPYPRVHIMDP  LKATF+QI++IQKP+ NP++  LL+
Sbjct: 81  ARVLPSIYKTIQNYGKGSYMWLGPYPRVHIMDPEQLKATFSQINDIQKPDMNPLIDYLLE 140

Query: 139 GVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMISDWERLISKEGWCEIDVMP 198
           G++T+EG+KWAKH+KII+PAF+L KLK MVP F+ +  E++S+WER++ +EG CE++VMP
Sbjct: 141 GIITHEGEKWAKHRKIINPAFHLDKLKGMVPAFVESSKEIVSEWERMVPEEGCCELNVMP 200

Query: 199 YLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAASRIYIPGWRFVPTKSNRKMK 258
           YL ++T D+ISRTA GSS+KEGQ+IF+LLK+L DLVV+ A  +YIPGWRF+PTKSN+K+K
Sbjct: 201 YLQDMTCDAISRTAFGSSYKEGQMIFQLLKQLIDLVVKVAFGVYIPGWRFLPTKSNKKLK 260

Query: 259 KIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESNLREIEEHGNNKRDGMSIEEV 318
           +I+ EI   ++GIINKRQKAM++GE   Q+DLLGILL+SN +EIE  GNNK  GMSIE+V
Sbjct: 261 EINEEIKKKVLGIINKRQKAMKEGE-AVQNDLLGILLDSNAKEIEAQGNNKHFGMSIEDV 320

Query: 319 VKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEVLQVFGNKDPTFDGLSSLKVV 378
           +KEC+IFY+ GQETTA LL WTM+ LS H +WQ+RAR EV +VFGN  P  DGL+ LKVV
Sbjct: 321 IKECKIFYIGGQETTAQLLTWTMVLLSFHTEWQERARAEVREVFGNDTPNSDGLNRLKVV 380

Query: 379 TMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCIPVILIHRDREIWGDDAHEFK 438
            MI++EV+RLYPPA+ + R V+KET +G L +P+GV+L  PVILIHRDREIWG+DA+EFK
Sbjct: 381 NMILHEVLRLYPPASMLLREVKKETSVGKLNVPAGVMLIAPVILIHRDREIWGEDANEFK 440

Query: 439 PERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKIVFSIILQHFSFELSSSYTHA 498
           PERFS G+S ASK QP F PF WGPRICMGQNFA+MEAK+  S+ILQ FSFELS SY HA
Sbjct: 441 PERFSNGVSKASKLQPAFFPFGWGPRICMGQNFAIMEAKVAISMILQRFSFELSPSYAHA 500

Query: 499 PTSIMTTQPQHGAHIILRKL 518
           PT +M+  PQHGAHIILRKL
Sbjct: 501 PTVVMSVGPQHGAHIILRKL 518

BLAST of MC11g_new0169 vs. NCBI nr
Match: XP_022156576.1 (cytochrome P450 CYP72A219-like [Momordica charantia])

HSP 1 Score: 702 bits (1811), Expect = 2.58e-249
Identity = 333/516 (64.53%), Postives = 422/516 (81.78%), Query Frame = 0

Query: 4   SVEVVVSICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELAAASG 63
           SV + +++C+V+ +   WRV DW+WLRPK+L+N+L  QGFAGNSY+I  GDLK  +AA+ 
Sbjct: 10  SVWITIAVCLVVGA---WRVVDWIWLRPKKLENYLRNQGFAGNSYQILHGDLKYRSAAND 69

Query: 64  QARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFTQIDE 123
           +A S PM  FS+ IAPRVVPS ++TIQ YGKNS+MW+GP PRVHIM+   +K  +  I++
Sbjct: 70  RANSKPM-NFSNHIAPRVVPSVHQTIQIYGKNSFMWIGPIPRVHIMEAEQVKTAYMLIND 129

Query: 124 IQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMISDWE 183
           IQKP+ NP+VKLL DG++ +EG KW KH+KII+PAF+L KLKDMVP F H+CNEM+  WE
Sbjct: 130 IQKPSINPLVKLLADGLVNHEGAKWVKHRKIINPAFHLEKLKDMVPAFSHSCNEMVDKWE 189

Query: 184 RLISKEGWCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAASRIYI 243
           R++S EG CE+DVMPYL ++TAD ISRTA GSS+++G+ IF+L KEL  L +++  RIYI
Sbjct: 190 RMVSNEGLCELDVMPYLQSMTADIISRTAFGSSYEKGKRIFELQKELGVLAIKSTLRIYI 249

Query: 244 PGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESNLREIE 303
           PG RF+PTKSN KMK++D+EI  L++GIIN+RQKAME GE    +DLLGIL+ESNL EI 
Sbjct: 250 PGSRFLPTKSNTKMKRMDKEIKGLILGIINERQKAMEAGE-AIHNDLLGILMESNLNEIR 309

Query: 304 EHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEVLQVFG 363
           +HGNNK  GMSIE+V++EC++FYLAGQETTA+LLIWTM+ LS H +WQ+RAR EVLQVFG
Sbjct: 310 QHGNNKDVGMSIEDVIEECKLFYLAGQETTATLLIWTMILLSSHSEWQERARAEVLQVFG 369

Query: 364 NKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCIPVILI 423
           N++P FDGLS LKVVTMI+NEV+RLYPP   + R+VQKETR G LT+P+GV+L +P ILI
Sbjct: 370 NREPDFDGLSRLKVVTMILNEVLRLYPPVGMVSRLVQKETRFGQLTLPAGVMLGLPTILI 429

Query: 424 HRDREIWGDDAHEFKPERFSEGISNASKA-QPGFLPFAWGPRICMGQNFAMMEAKIVFSI 483
             DRE+WG+DA EFKP+RFSEG+S A+K  Q  F+PF WGPRIC+GQNFAM+EAK   +I
Sbjct: 430 QHDRELWGEDAQEFKPQRFSEGVSKATKNNQTAFIPFGWGPRICIGQNFAMIEAKTALAI 489

Query: 484 ILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL 518
           ILQ FSFELSSSYTHAPTS +T QPQHGAH++L KL
Sbjct: 490 ILQRFSFELSSSYTHAPTSSITIQPQHGAHVVLHKL 520

BLAST of MC11g_new0169 vs. NCBI nr
Match: XP_038877590.1 (cytochrome P450 CYP72A219-like isoform X1 [Benincasa hispida])

HSP 1 Score: 696 bits (1797), Expect = 3.59e-247
Identity = 330/510 (64.71%), Postives = 413/510 (80.98%), Query Frame = 0

Query: 11  ICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELAAASGQARSNPM 70
           I +VL+ L+GW++ DW+WLRPK+L+  L RQGFAGNSYRI  GDLK+ AA   +A S PM
Sbjct: 15  IIVVLLLLLGWKLMDWIWLRPKKLEKVLIRQGFAGNSYRILYGDLKDRAAMREKAISKPM 74

Query: 71  PTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFTQIDEIQKP--N 130
             FS+ IAPRV+PS + TIQNYGKNS+MWLGP PRVHIMDP  LK  F+ I++ QKP  N
Sbjct: 75  -NFSNHIAPRVIPSVHHTIQNYGKNSFMWLGPIPRVHIMDPEQLKIVFSLINDFQKPTRN 134

Query: 131 FNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMISDWERLISK 190
            NP+ KLL DG+L +EG KW KH+KII+PAF+L KLKDMVP F H+CNEM+  WE ++S 
Sbjct: 135 LNPLAKLLADGLLNHEGPKWVKHRKIINPAFHLEKLKDMVPTFYHSCNEMVRKWESMVSV 194

Query: 191 EGWCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAASRIYIPGWRF 250
           EG CE+DVMPYL  +TAD ISRTA GSS+++G+ IFKL  EL DLV+++   IYIPGWRF
Sbjct: 195 EGSCELDVMPYLQTMTADVISRTAFGSSYEKGKEIFKLQTELADLVIQSTFGIYIPGWRF 254

Query: 251 VPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESNLREIEEHGNN 310
           +PTKSN KMK+I +EI TL++GIIN+++K+M+ GE   Q+DLL IL+ESNL EI++HGNN
Sbjct: 255 LPTKSNNKMKEISKEITTLILGIINEKEKSMKTGE-AIQTDLLSILMESNLNEIKQHGNN 314

Query: 311 KRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEVLQVFGNKDPT 370
           K  GMSIE+V+ EC++FY+AGQETTA+LL+WTM+ LS + +WQ +AR EV ++FGNK P 
Sbjct: 315 KDIGMSIEDVIDECKLFYIAGQETTATLLVWTMILLSSYSEWQKQARAEVFEIFGNKKPD 374

Query: 371 FDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCIPVILIHRDRE 430
           +DGLS LKV+TMI+NEV+RLYPP +   R V+KETRLGNLT+ +GV+L +P +LI RD E
Sbjct: 375 YDGLSRLKVITMILNEVLRLYPPVSMFARFVKKETRLGNLTLLAGVMLGLPTVLIQRDPE 434

Query: 431 IWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKIVFSIILQHFS 490
           +WG+DA EFKPERFSEG+S A+K    F+PF WGPRIC+G NFAM+EAK+V S+ILQ FS
Sbjct: 435 LWGEDADEFKPERFSEGVSKATKNPGAFVPFGWGPRICIGLNFAMIEAKMVLSMILQRFS 494

Query: 491 FELSSSYTHAPTSIMTTQPQHGAHIILRKL 518
           FELSSSYTHAPT  MTTQPQHGAHIIL KL
Sbjct: 495 FELSSSYTHAPTVTMTTQPQHGAHIILHKL 522

BLAST of MC11g_new0169 vs. ExPASy TrEMBL
Match: A0A6J1D398 (cytochrome P450 CYP72A219-like OS=Momordica charantia OX=3673 GN=LOC111017168 PE=3 SV=1)

HSP 1 Score: 1055 bits (2729), Expect = 0.0
Identity = 518/518 (100.00%), Postives = 518/518 (100.00%), Query Frame = 0

Query: 1   METSVEVVVSICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELAA 60
           METSVEVVVSICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELAA
Sbjct: 1   METSVEVVVSICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELAA 60

Query: 61  ASGQARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFTQ 120
           ASGQARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFTQ
Sbjct: 61  ASGQARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFTQ 120

Query: 121 IDEIQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMIS 180
           IDEIQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMIS
Sbjct: 121 IDEIQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMIS 180

Query: 181 DWERLISKEGWCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAASR 240
           DWERLISKEGWCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAASR
Sbjct: 181 DWERLISKEGWCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAASR 240

Query: 241 IYIPGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESNLR 300
           IYIPGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESNLR
Sbjct: 241 IYIPGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESNLR 300

Query: 301 EIEEHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEVLQ 360
           EIEEHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEVLQ
Sbjct: 301 EIEEHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEVLQ 360

Query: 361 VFGNKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCIPV 420
           VFGNKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCIPV
Sbjct: 361 VFGNKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCIPV 420

Query: 421 ILIHRDREIWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKIVF 480
           ILIHRDREIWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKIVF
Sbjct: 421 ILIHRDREIWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKIVF 480

Query: 481 SIILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL 518
           SIILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL
Sbjct: 481 SIILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL 518

BLAST of MC11g_new0169 vs. ExPASy TrEMBL
Match: A0A6J1DVD0 (cytochrome P450 CYP72A219-like OS=Momordica charantia OX=3673 GN=LOC111023453 PE=3 SV=1)

HSP 1 Score: 702 bits (1811), Expect = 1.25e-249
Identity = 333/516 (64.53%), Postives = 422/516 (81.78%), Query Frame = 0

Query: 4   SVEVVVSICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELAAASG 63
           SV + +++C+V+ +   WRV DW+WLRPK+L+N+L  QGFAGNSY+I  GDLK  +AA+ 
Sbjct: 10  SVWITIAVCLVVGA---WRVVDWIWLRPKKLENYLRNQGFAGNSYQILHGDLKYRSAAND 69

Query: 64  QARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFTQIDE 123
           +A S PM  FS+ IAPRVVPS ++TIQ YGKNS+MW+GP PRVHIM+   +K  +  I++
Sbjct: 70  RANSKPM-NFSNHIAPRVVPSVHQTIQIYGKNSFMWIGPIPRVHIMEAEQVKTAYMLIND 129

Query: 124 IQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMISDWE 183
           IQKP+ NP+VKLL DG++ +EG KW KH+KII+PAF+L KLKDMVP F H+CNEM+  WE
Sbjct: 130 IQKPSINPLVKLLADGLVNHEGAKWVKHRKIINPAFHLEKLKDMVPAFSHSCNEMVDKWE 189

Query: 184 RLISKEGWCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAASRIYI 243
           R++S EG CE+DVMPYL ++TAD ISRTA GSS+++G+ IF+L KEL  L +++  RIYI
Sbjct: 190 RMVSNEGLCELDVMPYLQSMTADIISRTAFGSSYEKGKRIFELQKELGVLAIKSTLRIYI 249

Query: 244 PGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESNLREIE 303
           PG RF+PTKSN KMK++D+EI  L++GIIN+RQKAME GE    +DLLGIL+ESNL EI 
Sbjct: 250 PGSRFLPTKSNTKMKRMDKEIKGLILGIINERQKAMEAGE-AIHNDLLGILMESNLNEIR 309

Query: 304 EHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEVLQVFG 363
           +HGNNK  GMSIE+V++EC++FYLAGQETTA+LLIWTM+ LS H +WQ+RAR EVLQVFG
Sbjct: 310 QHGNNKDVGMSIEDVIEECKLFYLAGQETTATLLIWTMILLSSHSEWQERARAEVLQVFG 369

Query: 364 NKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCIPVILI 423
           N++P FDGLS LKVVTMI+NEV+RLYPP   + R+VQKETR G LT+P+GV+L +P ILI
Sbjct: 370 NREPDFDGLSRLKVVTMILNEVLRLYPPVGMVSRLVQKETRFGQLTLPAGVMLGLPTILI 429

Query: 424 HRDREIWGDDAHEFKPERFSEGISNASKA-QPGFLPFAWGPRICMGQNFAMMEAKIVFSI 483
             DRE+WG+DA EFKP+RFSEG+S A+K  Q  F+PF WGPRIC+GQNFAM+EAK   +I
Sbjct: 430 QHDRELWGEDAQEFKPQRFSEGVSKATKNNQTAFIPFGWGPRICIGQNFAMIEAKTALAI 489

Query: 484 ILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL 518
           ILQ FSFELSSSYTHAPTS +T QPQHGAH++L KL
Sbjct: 490 ILQRFSFELSSSYTHAPTSSITIQPQHGAHVVLHKL 520

BLAST of MC11g_new0169 vs. ExPASy TrEMBL
Match: A0A5A7VAQ5 (Cytochrome P450 CYP72A219-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G00130 PE=3 SV=1)

HSP 1 Score: 696 bits (1795), Expect = 3.37e-247
Identity = 324/516 (62.79%), Postives = 419/516 (81.20%), Query Frame = 0

Query: 5   VEVVVS-ICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELAAASG 64
           + V++S + ++L+ L+GW++ DW+W RPK+L+  L RQGFAGNSYRI  GDLKE AA   
Sbjct: 8   IGVIISYVLLLLLLLLGWKLVDWIWFRPKKLEKILRRQGFAGNSYRILHGDLKESAAMRE 67

Query: 65  QARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFTQIDE 124
           +A S PM  FS+ IAPRV+PS Y TIQ YGK S MWLGP PRVHIMDP  LK  F+ I++
Sbjct: 68  EAMSKPM-NFSNHIAPRVIPSVYHTIQLYGKKSMMWLGPMPRVHIMDPEQLKIVFSSIND 127

Query: 125 IQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTC-NEMISDW 184
            QKP  NP+ +LL+ G+ + EGQKW KH+KII+PAF++ KLKDMVP F H+C NEM+S W
Sbjct: 128 FQKPTMNPLTRLLVQGLASLEGQKWVKHRKIINPAFHVEKLKDMVPAFYHSCYNEMVSKW 187

Query: 185 ERLISKEGWCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAASRIY 244
           E ++S+EG CE+DVMPYL N+ +D ISRTA GSS+++G+ IF+L  EL +LV++A   IY
Sbjct: 188 EGMVSREGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQTELANLVIQANLGIY 247

Query: 245 IPGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESNLREI 304
           IPGWRF+PTKSNRKMK+I +EI TL++GI+N+++K+++ GE   Q+DLL IL+ESN+ EI
Sbjct: 248 IPGWRFLPTKSNRKMKEISKEITTLILGIMNEKEKSIKAGE-AIQTDLLSILMESNMNEI 307

Query: 305 EEHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEVLQVF 364
           ++HGNNK  GMSIE+V++EC++FY+AGQETTA+LL+WTM+ LS + +WQ+RAR EV ++F
Sbjct: 308 KQHGNNKNIGMSIEDVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQERARAEVFEIF 367

Query: 365 GNKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCIPVIL 424
           GNK P +DGL+ LKVVTMI NEV+RLYPP N   R+++KETRLGNLT+P GV+L +PV+L
Sbjct: 368 GNKKPDYDGLNRLKVVTMIFNEVLRLYPPVNMFGRIIRKETRLGNLTLPEGVMLGLPVVL 427

Query: 425 IHRDREIWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKIVFSI 484
           IH D E+WG+DAHEF PERFSEGIS A+K    F+PF WGPRIC+GQNFAM+EAK+  SI
Sbjct: 428 IHLDPELWGEDAHEFNPERFSEGISKATKNPSAFIPFGWGPRICIGQNFAMIEAKMALSI 487

Query: 485 ILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL 518
           ILQ FSFELS SYTHAP +++TTQPQHGAHIIL KL
Sbjct: 488 ILQRFSFELSPSYTHAPIAVLTTQPQHGAHIILHKL 521

BLAST of MC11g_new0169 vs. ExPASy TrEMBL
Match: A0A1S3BTS9 (cytochrome P450 CYP72A219-like OS=Cucumis melo OX=3656 GN=LOC103493473 PE=3 SV=1)

HSP 1 Score: 696 bits (1795), Expect = 3.37e-247
Identity = 324/516 (62.79%), Postives = 419/516 (81.20%), Query Frame = 0

Query: 5   VEVVVS-ICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELAAASG 64
           + V++S + ++L+ L+GW++ DW+W RPK+L+  L RQGFAGNSYRI  GDLKE AA   
Sbjct: 8   IGVIISYVLLLLLLLLGWKLVDWIWFRPKKLEKILRRQGFAGNSYRILHGDLKESAAMRE 67

Query: 65  QARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFTQIDE 124
           +A S PM  FS+ IAPRV+PS Y TIQ YGK S MWLGP PRVHIMDP  LK  F+ I++
Sbjct: 68  EAMSKPM-NFSNHIAPRVIPSVYHTIQLYGKKSMMWLGPMPRVHIMDPEQLKIVFSSIND 127

Query: 125 IQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTC-NEMISDW 184
            QKP  NP+ +LL+ G+ + EGQKW KH+KII+PAF++ KLKDMVP F H+C NEM+S W
Sbjct: 128 FQKPTMNPLTRLLVQGLASLEGQKWVKHRKIINPAFHVEKLKDMVPAFYHSCYNEMVSKW 187

Query: 185 ERLISKEGWCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAASRIY 244
           E ++S+EG CE+DVMPYL N+ +D ISRTA GSS+++G+ IF+L  EL +LV++A   IY
Sbjct: 188 EGMVSREGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQTELANLVIQANLGIY 247

Query: 245 IPGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESNLREI 304
           IPGWRF+PTKSNRKMK+I +EI TL++GI+N+++K+++ GE   Q+DLL IL+ESN+ EI
Sbjct: 248 IPGWRFLPTKSNRKMKEISKEITTLILGIMNEKEKSIKAGE-AIQTDLLSILMESNMNEI 307

Query: 305 EEHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEVLQVF 364
           ++HGNNK  GMSIE+V++EC++FY+AGQETTA+LL+WTM+ LS + +WQ+RAR EV ++F
Sbjct: 308 KQHGNNKNIGMSIEDVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQERARAEVFEIF 367

Query: 365 GNKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCIPVIL 424
           GNK P +DGL+ LKVVTMI NEV+RLYPP N   R+++KETRLGNLT+P GV+L +PV+L
Sbjct: 368 GNKKPDYDGLNRLKVVTMIFNEVLRLYPPVNMFGRIIRKETRLGNLTLPEGVMLGLPVVL 427

Query: 425 IHRDREIWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKIVFSI 484
           IH D E+WG+DAHEF PERFSEGIS A+K    F+PF WGPRIC+GQNFAM+EAK+  SI
Sbjct: 428 IHLDPELWGEDAHEFNPERFSEGISKATKNPSAFIPFGWGPRICIGQNFAMIEAKMALSI 487

Query: 485 ILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL 518
           ILQ FSFELS SYTHAP +++TTQPQHGAHIIL KL
Sbjct: 488 ILQRFSFELSPSYTHAPIAVLTTQPQHGAHIILHKL 521

BLAST of MC11g_new0169 vs. ExPASy TrEMBL
Match: A0A6J1F7R4 (cytochrome P450 CYP72A219-like OS=Cucurbita moschata OX=3662 GN=LOC111442910 PE=3 SV=1)

HSP 1 Score: 690 bits (1781), Expect = 4.53e-245
Identity = 322/500 (64.40%), Postives = 408/500 (81.60%), Query Frame = 0

Query: 19  VGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELAAASGQARSNPMPTFSHDIA 78
           V W   +W+W+RP++L+  L  QG AGN YRI  GDLKE  A   +A S PM  FSHDI 
Sbjct: 21  VSWGALNWVWIRPRKLEKRLREQGLAGNPYRILYGDLKESLALLKEANSKPM-AFSHDIG 80

Query: 79  PRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFTQIDEIQKPNFNPIVKLLLD 138
            RV+PS    IQN+GKNSY+WLGPYPRV+IMDP  L ATF+ I++IQKP+ NP  K LL+
Sbjct: 81  ARVLPSISNAIQNHGKNSYIWLGPYPRVNIMDPEQLNATFSLINDIQKPDMNPFFKFLLE 140

Query: 139 GVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMISDWERLISKEGWCEIDVMP 198
           G++T+EG+KWAKH+KII+PAF+L KLK+MVP F+ + NE++S+WER++ ++G CE+DVMP
Sbjct: 141 GIITHEGEKWAKHRKIINPAFHLDKLKNMVPVFVDSSNEIVSEWERIVPEDGCCELDVMP 200

Query: 199 YLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAASRIYIPGWRFVPTKSNRKMK 258
           YL +LT D+ISR A GSS+KEGQ+IF+LLK+LTD VV+ A  IYIPGWRF+PTKSN K+K
Sbjct: 201 YLQSLTRDAISRAAFGSSYKEGQMIFQLLKQLTDWVVKVAGGIYIPGWRFLPTKSNNKLK 260

Query: 259 KIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESNLREIEEHGNNKRDGMSIEEV 318
           + + EI  L++GIINKRQKAM++GE   Q+DLLGILL+SN +EIE  GNNK  GMSIE+V
Sbjct: 261 ETNEEIKRLVLGIINKRQKAMKEGE-AVQNDLLGILLDSNAKEIEAQGNNKDVGMSIEDV 320

Query: 319 VKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEVLQVFGNKDPTFDGLSSLKVV 378
           +KEC+IFY+ GQETTA LL W+M+ LS++ +WQ+RAR EV +VFG+  P  DGLS LKVV
Sbjct: 321 IKECKIFYIGGQETTAQLLTWSMILLSVYTEWQERARAEVREVFGDNTPNSDGLSRLKVV 380

Query: 379 TMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCIPVILIHRDREIWGDDAHEFK 438
            MI++EV+RLYPPA+ + RVV+KETR+G L +P+GV+L +P++LIHRDREIWG+DA+EFK
Sbjct: 381 NMILHEVLRLYPPASMLPRVVKKETRVGKLNLPAGVMLVVPMVLIHRDREIWGEDANEFK 440

Query: 439 PERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKIVFSIILQHFSFELSSSYTHA 498
           PERFS G+SNASK QP FLPF WGPRIC+GQNFA+ EAKI  S+ILQ FSFELS SYTHA
Sbjct: 441 PERFSNGVSNASKQQPAFLPFGWGPRICLGQNFAIFEAKIALSLILQRFSFELSPSYTHA 500

Query: 499 PTSIMTTQPQHGAHIILRKL 518
           P  +M+ +PQHGAHIILRKL
Sbjct: 501 PIVVMSIEPQHGAHIILRKL 518

BLAST of MC11g_new0169 vs. TAIR 10
Match: AT3G14610.1 (cytochrome P450, family 72, subfamily A, polypeptide 7 )

HSP 1 Score: 562.0 bits (1447), Expect = 5.0e-160
Identity = 266/520 (51.15%), Postives = 372/520 (71.54%), Query Frame = 0

Query: 1   METSVEVVVSICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELAA 60
           M  SV   + + + ++ L  WR+  W+W++PK L++ L RQG  G  Y   VGD+K    
Sbjct: 1   MSFSVVAALPVLVAVVVLWTWRIVKWVWIKPKMLESSLKRQGLTGTPYTPLVGDIKRNVD 60

Query: 61  ASGQARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFTQ 120
              +ARS P+   + DI PR++P   K + ++GK  ++W+GP P + I +P  +K  F +
Sbjct: 61  MMMEARSKPI-NVTDDITPRLLPLALKMLNSHGKTFFIWIGPLPTIVITNPEQIKEVFNK 120

Query: 121 IDEIQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMIS 180
           +++ +K +  P+++LL  G+ +Y+G KWA H++II+PAF+L K+K+M+P F H C+E++ 
Sbjct: 121 VNDFEKASTFPLIRLLAGGLASYKGDKWASHRRIINPAFHLEKIKNMIPAFYHCCSEVVC 180

Query: 181 DWERLIS-KEGWCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAAS 240
            WE+L + KE   E+DV P+L N+TAD IS TA GSS+KEGQ IF+L  EL +L+ +A  
Sbjct: 181 QWEKLFTDKESPLEVDVWPWLVNMTADVISHTAFGSSYKEGQRIFQLQGELAELIAQAFK 240

Query: 241 RIYIPGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESNL 300
           + YIPG RF PTKSNR+MK IDRE++ +L GI++KR+KA E GE  A  DLLGILLESN 
Sbjct: 241 KSYIPGSRFYPTKSNRRMKAIDREVDVILRGIVSKREKAREAGE-PANDDLLGILLESNS 300

Query: 301 REIEEHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEVL 360
            E      ++ +GMS+E+V+KEC++FY AGQETT+ LL+WTM+ LS H+ WQ RAR EV+
Sbjct: 301 EE------SQGNGMSVEDVMKECKLFYFAGQETTSVLLVWTMVLLSHHQDWQARAREEVM 360

Query: 361 QVFG-NKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCI 420
           QV G N  P  + L++LKV+TMI NEV+RLYPP   + RVV KE +LG LT+P+G+ + +
Sbjct: 361 QVLGENNKPDMESLNNLKVMTMIFNEVLRLYPPVAQLKRVVNKEMKLGELTLPAGIQIYL 420

Query: 421 PVILIHRDREIWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKI 480
           P IL+ RD E+WGDDA +FKPERF +G+S A+K Q  F PF WGPRIC+GQNFAM+EAK+
Sbjct: 421 PTILVQRDTELWGDDAADFKPERFRDGLSKATKNQVSFFPFGWGPRICIGQNFAMLEAKM 480

Query: 481 VFSIILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL 519
             ++ILQ FSFELS SY HAP ++MTT+PQ GAH+IL KL
Sbjct: 481 AMALILQKFSFELSPSYVHAPQTVMTTRPQFGAHLILHKL 512

BLAST of MC11g_new0169 vs. TAIR 10
Match: AT3G14660.1 (cytochrome P450, family 72, subfamily A, polypeptide 13 )

HSP 1 Score: 556.6 bits (1433), Expect = 2.1e-158
Identity = 265/520 (50.96%), Postives = 373/520 (71.73%), Query Frame = 0

Query: 1   METSV-EVVVSICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELA 60
           ME SV  V VS+ +V++S   WR    +WL+PK L+++L RQG AG  Y   VGDLK   
Sbjct: 1   MEISVASVTVSVAVVVVSWWVWRTLQRVWLKPKMLESYLRRQGLAGTPYTPLVGDLKRNF 60

Query: 61  AASGQARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFT 120
           +   +ARS P+   + DI PR+VP   + ++ +G+  + W GP P + IMDP  +K  F 
Sbjct: 61  SMLAEARSKPI-NLTDDITPRIVPYPLQMLKTHGRTFFTWFGPIPTITIMDPEQIKEVFN 120

Query: 121 QIDEIQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMI 180
           ++ + QK +  P+ +L+  G+++Y+G KW KH++II+PAF+L K+K+MVP F  +C+E++
Sbjct: 121 KVYDFQKAHTFPLGRLIAAGLVSYDGDKWTKHRRIINPAFHLEKIKNMVPAFHQSCSEIV 180

Query: 181 SDWERLIS-KEGWCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAA 240
            +W++L++ K+  CE+D+ P+L ++TAD ISRTA GSS+KEGQ IF+L  EL  L+++A 
Sbjct: 181 GEWDKLVTDKQSSCEVDIWPWLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAF 240

Query: 241 SRIYIPGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESN 300
            +  IPG+R+ PTK NR+MK   REI  +L GI+NKR +A E GE     DLLGILLESN
Sbjct: 241 RKAIIPGYRYFPTKGNRRMKAAAREIKFILRGIVNKRLRAREAGE-APSDDLLGILLESN 300

Query: 301 LREIEEHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEV 360
           L      G  K +GMS EE+++EC++FY AGQETT  LL+WTM+ LS H+ WQ RAR EV
Sbjct: 301 L------GQTKGNGMSTEELMEECKLFYFAGQETTTVLLVWTMVLLSQHQDWQARAREEV 360

Query: 361 LQVFGNKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCI 420
            QVFG+K+P  +GL+ LKV+TMI+ EV+RLYPP   + R + KE +LG+LT+P GV + +
Sbjct: 361 KQVFGDKEPDAEGLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMQLGDLTLPGGVQISL 420

Query: 421 PVILIHRDREIWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKI 480
           P++LI RDRE+WG+DA EFKP+RF +G+S A+K Q  F PFAWGPRIC+GQNFA++EAK+
Sbjct: 421 PILLIQRDRELWGNDAGEFKPDRFKDGLSKATKNQVSFFPFAWGPRICIGQNFALLEAKM 480

Query: 481 VFSIILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL 519
             ++IL+ FSFELS SY HAP +++TT PQ GA +IL KL
Sbjct: 481 AMTLILRKFSFELSPSYVHAPYTVLTTHPQFGAPLILHKL 512

BLAST of MC11g_new0169 vs. TAIR 10
Match: AT3G14690.1 (cytochrome P450, family 72, subfamily A, polypeptide 15 )

HSP 1 Score: 555.1 bits (1429), Expect = 6.1e-158
Identity = 261/520 (50.19%), Postives = 371/520 (71.35%), Query Frame = 0

Query: 1   METSV-EVVVSICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELA 60
           ME SV  V +S+ + ++S   WR   W+W +PK L+++L RQG AG  Y   VGDLK+  
Sbjct: 1   MEISVASVTISVVLAVVSWWIWRTLQWVWFKPKMLEHYLRRQGLAGTPYTPLVGDLKKNF 60

Query: 61  AASGQARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFT 120
               +ARS P+   + DI+PRVVP   +  + YG+  + W GP P + IMDP  +K  F 
Sbjct: 61  TMLSEARSKPL-KLTDDISPRVVPYPLQMFKTYGRTYFTWFGPIPTITIMDPEQIKEVFN 120

Query: 121 QIDEIQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMI 180
           ++ + QKP+  P+  ++  G+  Y+G KWAKH++II+PAF++ K+K+MVP F  +C E++
Sbjct: 121 KVYDFQKPHTFPLATIIAKGLANYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCREVV 180

Query: 181 SDWERLISKEG-WCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAA 240
            +W++L+S +G  CE+DV P L ++TAD ISRTA GSS+KEGQ IF+L  EL  L+++A 
Sbjct: 181 GEWDQLVSDKGSSCEVDVWPGLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAF 240

Query: 241 SRIYIPGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESN 300
            + +IPG+ ++PTKSNR+MK   REI  +L GI+NKR +A E GE     DLLGILLESN
Sbjct: 241 RKAFIPGYSYLPTKSNRRMKAAAREIQVILRGIVNKRLRAREAGE-APSDDLLGILLESN 300

Query: 301 LREIEEHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEV 360
           LR+ E  GN    GMS E++++EC++FY AGQETT+ LL+WTM+ LS H+ WQ RAR EV
Sbjct: 301 LRQTE--GN----GMSTEDLMEECKLFYFAGQETTSVLLVWTMVLLSQHQDWQARAREEV 360

Query: 361 LQVFGNKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCI 420
            QVFG+K+P  +GL+ LKV+TMI+ EV+RLYPP   + R + KE +LG+LT+P GV + +
Sbjct: 361 KQVFGDKEPDAEGLNQLKVMTMILYEVLRLYPPVTQLTRAIHKELKLGDLTLPGGVQISL 420

Query: 421 PVILIHRDREIWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKI 480
           P++L+  D E+WG+DA EF P+RF +G+S A+K+Q  F PFAWGPRIC+GQNFA++EAK+
Sbjct: 421 PILLVQHDIELWGNDAAEFNPDRFKDGLSKATKSQVSFFPFAWGPRICIGQNFALLEAKM 480

Query: 481 VFSIILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL 519
             ++IL+ FSFE+S SY HAP +++T  PQ GA +I+ KL
Sbjct: 481 AMALILRRFSFEISPSYVHAPYTVITIHPQFGAQLIMHKL 512

BLAST of MC11g_new0169 vs. TAIR 10
Match: AT3G14640.1 (cytochrome P450, family 72, subfamily A, polypeptide 10 )

HSP 1 Score: 554.3 bits (1427), Expect = 1.0e-157
Identity = 262/521 (50.29%), Postives = 371/521 (71.21%), Query Frame = 0

Query: 1   METSVE-VVVSICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELA 60
           ME SV  V VS+ +V++S   WR   W+W +PK L+++L RQG AG  Y   +GDLK   
Sbjct: 1   MEISVACVTVSVVVVVVSWWVWRTLQWVWFKPKMLESYLRRQGLAGTPYTPLIGDLKRNV 60

Query: 61  AASGQARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFT 120
               +A S P+   + DI PRV+P  ++ ++ +G+  + WLGP P + IMDP  +K  F 
Sbjct: 61  NMLTEATSKPI-KLTEDITPRVLPHPFQMLKTHGRTFFTWLGPKPTITIMDPELIKEVFN 120

Query: 121 QIDEIQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMI 180
           ++ +  K     + +L+  G++ Y+G KWAKH++II+PAF++ K+K+MVP F  +C++++
Sbjct: 121 KVYDYPKAQTFLLGRLIATGIINYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCSDVV 180

Query: 181 SDWERLISKEG--WCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEA 240
            +W +L+S +G   CE+DV P+L ++T D ISRTA GSS+KEGQ IF+L  EL  L+++A
Sbjct: 181 GEWSKLVSDKGSSSCEVDVWPWLVSMTGDVISRTAFGSSYKEGQRIFELQAELVHLILQA 240

Query: 241 ASRIYIPGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLES 300
             ++YIPG+R++PTKSNR+MK   REI  +L GI+NKR +A E G+     DLLGILLES
Sbjct: 241 FWKVYIPGYRYLPTKSNRRMKAAAREIQVILKGIVNKRLRAREAGKAAPNDDLLGILLES 300

Query: 301 NLREIEEHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVE 360
           NL      G  K +GMS E+V++EC++FY AGQETT+ LL+W M+ LS H+ WQ RAR E
Sbjct: 301 NL------GQAKGNGMSTEDVMEECKLFYFAGQETTSVLLVWAMVLLSHHQDWQARAREE 360

Query: 361 VLQVFGNKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILC 420
           V QVFG+K+P  + LS LKV+TMI+ EV+RLYPP   + R + KE +LG+LT+P+GV + 
Sbjct: 361 VKQVFGDKEPDTECLSQLKVMTMILYEVLRLYPPVTHLTRAIDKEMKLGDLTLPAGVHIS 420

Query: 421 IPVILIHRDREIWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAK 480
           +P++L+ RD  +WG DA EFKPERF +G+S A+K+Q  F PFAWGPRIC+GQNFAM+EAK
Sbjct: 421 LPIMLVQRDPMLWGTDAAEFKPERFKDGLSKATKSQVSFFPFAWGPRICIGQNFAMLEAK 480

Query: 481 IVFSIILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL 519
           +  ++ILQ F+FELS SY HAP +++T  PQ GAH+ILRKL
Sbjct: 481 MAMALILQTFTFELSPSYVHAPQTVVTIHPQFGAHLILRKL 514

BLAST of MC11g_new0169 vs. TAIR 10
Match: AT3G14680.1 (cytochrome P450, family 72, subfamily A, polypeptide 14 )

HSP 1 Score: 548.9 bits (1413), Expect = 4.4e-156
Identity = 263/520 (50.58%), Postives = 372/520 (71.54%), Query Frame = 0

Query: 1   METSV-EVVVSICMVLMSLVGWRVFDWLWLRPKRLQNFLTRQGFAGNSYRIFVGDLKELA 60
           ME SV  V  S+ +V++S   WR   W+W  PK L+  L RQG +G SY   +GD K++ 
Sbjct: 1   MEISVSSVTFSLAVVVVSWWVWRTLKWVWFTPKMLERSLRRQGLSGTSYTPLIGDFKKMI 60

Query: 61  AASGQARSNPMPTFSHDIAPRVVPSEYKTIQNYGKNSYMWLGPYPRVHIMDPTHLKATFT 120
           +   +A S P+   + DI PRV+P   + ++ +G+ +  W GP P + IMDP  +K  F 
Sbjct: 61  SMFIEATSKPIKP-TDDITPRVMPHPLQMLKTHGRTNLTWFGPIPTITIMDPEQIKEVFN 120

Query: 121 QIDEIQKPNFNPIVKLLLDGVLTYEGQKWAKHKKIISPAFYLLKLKDMVPKFIHTCNEMI 180
           ++ + QK +  P+ K+L  G+++Y+G KWA+H++II+PAF+L K+K+MV  F  +C+E++
Sbjct: 121 KVYDFQKAHTFPLSKILGTGLVSYDGDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELV 180

Query: 181 SDWERLISKEG-WCEIDVMPYLHNLTADSISRTACGSSFKEGQIIFKLLKELTDLVVEAA 240
            +W++L+S +G  CE+DV P L ++TAD ISRTA GSS++EG  IF+L  EL  LV++A 
Sbjct: 181 GEWDKLVSDKGSSCEVDVWPGLTSMTADVISRTAFGSSYREGHRIFELQAELAQLVMQAF 240

Query: 241 SRIYIPGWRFVPTKSNRKMKKIDREINTLLMGIINKRQKAMEKGELGAQSDLLGILLESN 300
            + +IPG+ ++PTK NR+MK   REI  +L GIINKR++A E GE  ++ DLLGILLESN
Sbjct: 241 QKFFIPGYIYLPTKGNRRMKTAAREIQDILRGIINKRERARESGEAPSE-DLLGILLESN 300

Query: 301 LREIEEHGNNKRDGMSIEEVVKECRIFYLAGQETTASLLIWTMLCLSLHKQWQDRARVEV 360
           L      G  + +GMS E++++EC++FYLAGQETT+ LL+WTM+ LS H+ WQ RAR EV
Sbjct: 301 L------GQTEGNGMSTEDMMEECKLFYLAGQETTSVLLVWTMVLLSQHQDWQARAREEV 360

Query: 361 LQVFGNKDPTFDGLSSLKVVTMIMNEVMRLYPPANTIHRVVQKETRLGNLTIPSGVILCI 420
            QVFG+K P  +GL+ LKV+TMI+ EV+RLYPP   + R + KE +LG+LT+P GV + +
Sbjct: 361 KQVFGDKQPDTEGLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMKLGDLTLPGGVQISL 420

Query: 421 PVILIHRDREIWGDDAHEFKPERFSEGISNASKAQPGFLPFAWGPRICMGQNFAMMEAKI 480
           PV+L+HRD E+WG+DA EFKPERF +G+S A+K Q  F PFAWGPRIC+GQNF ++EAK+
Sbjct: 421 PVLLVHRDTELWGNDAGEFKPERFKDGLSKATKNQVSFFPFAWGPRICIGQNFTLLEAKM 480

Query: 481 VFSIILQHFSFELSSSYTHAPTSIMTTQPQHGAHIILRKL 519
             S+ILQ FSFELS SY HAP +I+T  PQ GAH++L KL
Sbjct: 481 AMSLILQRFSFELSPSYVHAPYTIITLYPQFGAHLMLHKL 512