MC11g_new0113 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g_new0113 vs. ExPASy Swiss-Prot
Match: A5ABH4 (Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rglB PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.7e-19
Identity = 99/376 (26.33%), Postives = 153/376 (40.69%), Query Frame = 0

Query: 48  KYQYSCENKDLKVHGWIC----TNPPT-GFWQITPSDEFRSGGPLKQSLTSHVGPTTLAI 107
           KY +S   +D  VHG       +N  T G W +  + +   GGPL   LT  V       
Sbjct: 216 KYTFSNAWRDNSVHGMYADGSTSNGSTFGAWLVMNTKDTYYGGPLHSDLT--VDGIVYNY 275

Query: 108 FLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYFNSTSVGDDPFWLWEDAKIQMMTEVQ-SW 167
            +S H+   +  P    G  + + FGP + +FN    G       ++ K    T    SW
Sbjct: 276 LVSNHHG--EGTPNITYG--FDRTFGPQYYHFNG---GKGSTASLQELKSDAETLADPSW 335

Query: 168 PYSFPAS-----EDFPKVDQRGTVSGRLLVFDRYVSQDYLP--ASGAFVGLSAPGEVGSW 227
              F  S       +    QRG+V G++           LP  A+     L+  G+    
Sbjct: 336 NVDFYDSIAKHVVGYTPSSQRGSVQGKI----------KLPKGATRPIAVLTVDGQYFQD 395

Query: 228 QR-ECKGYQFWTRADDGGYFTINHIHAGDYNLYAWVHGFIGDYRCDALININQGSCIEMG 287
                  YQ+W   DD G+F+++H+  G Y L  +  G  GD+  D  + +  G    + 
Sbjct: 396 NSVNSSSYQYWAEIDDSGHFSVDHVKEGPYRLTVYADGIFGDFVRDG-VQVKAGKKTTIQ 455

Query: 288 DLVYEPPRDGPTLWEIGIPDRSASEF---YAPDP-NPIHINNLFVNHPDRFRQYGLWDRY 347
           +  +E    G  +W +G PD+S+ EF    A DP +P+H        P+    +G +D  
Sbjct: 456 E-TWEAESAGTEIWRLGTPDKSSGEFRHGVARDPTHPLH-------PPEYLIYWGAYDWQ 515

Query: 348 AELYPDEDLVYTVGTSDYSKD-----W-FFAQVPRKTNSNAHQGTTWQIKFKL--DSVDH 398
           ++ +PD  + YT+GTSD + D     W  F   P            W I F L  D +  
Sbjct: 516 SD-FPD-GINYTIGTSDPATDLNTVHWSVFGPTPNDPRVEYDTTHDWTINFPLSEDDLAE 561

BLAST of MC11g_new0113 vs. ExPASy Swiss-Prot
Match: Q5AZ85 (Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglB PE=2 SV=2)

HSP 1 Score: 87.4 bits (215), Expect = 4.7e-16
Identity = 111/474 (23.42%), Postives = 178/474 (37.55%), Query Frame = 0

Query: 48  KYQYSCENKDLKVHGWICTNPPT-----GFWQITPSDEFRSGGPLKQSLTSHVGPTTLAI 107
           KY +S   +D  VHG       +     G W +  + +   GGPL   LT  V       
Sbjct: 215 KYTFSNHWRDNDVHGLYADGSTSDGTTYGAWLVMNTKDTYYGGPLHSDLT--VDGIIYNY 274

Query: 108 FLSAHYAGQDLVPKFRAGEPWKKVFGPVFLYFNSTSVGDDPFWLWEDAKIQMMTEVQSWP 167
            +S H+   +  P    G  + + FGP F  FN    G     L E           SW 
Sbjct: 275 IVSNHHG--EGTPNITNG--FDRTFGPQFYLFN----GGGSSSLEELRDEARSLASPSWN 334

Query: 168 YSFPAS-----EDFPKVDQRGTVSGRLLVFDRYVSQDYLP--ASGAFVGLSAPGEVGSWQ 227
             F  S       +    QRG+V G +           LP  A      L+  G      
Sbjct: 335 ADFYDSIAKHVIGYVPSSQRGSVKGTI----------KLPKNAKSPIAVLTVDGHYFQDN 394

Query: 228 REC-KGYQFWTRADDGGYFTINHIHAGDYNLYAWVHGFIGDYRCDAL-ININQGSCIEMG 287
                 +Q+W   D  G F+I+ + AG Y L  +  G  GD+  D + +   + + I+  
Sbjct: 395 SAVPSSHQYWADIDKNGRFSIDRVVAGKYRLTVYADGIFGDFTRDGIVVKARKSTSIKE- 454

Query: 288 DLVYEPPRDGPTLWEIGIPDRSASEF-YAPDPNPIHINNLFVNHPDRFRQY-GLWDRYAE 347
              ++P   G  +W +G PD+S+ EF +    +P H       HP  +  Y G +D  ++
Sbjct: 455 --TWKPESAGTEIWRLGTPDKSSGEFRHGAARDPTH-----PRHPPEYLIYWGAYDWQSD 514

Query: 348 LYPDEDLVYTVGTSDYSKDW------FFAQVPRKTNSNAHQGTTWQIKFKLDS--VDHKS 407
                D  Y +G SD + D+       F   P    + ++    W+I+F L +  +  + 
Sbjct: 515 FPGGID--YMIGESDPATDFNTVHWAVFGPTPDNPVAESNTTHDWRIRFDLSAKQLHARK 574

Query: 408 MYMLRVAIASATLAELQVRI---NDPRSKRPLFT---------SGLIGRDNS---IARHG 467
              L + +A A  A     +   ++P +  PL +         + +IG D S   I R  
Sbjct: 575 TATLTIQLAGAKAASGNTDVYNASEPYANLPLRSYINEQEEPLTMVIGYDQSSSCIVRSA 634

Query: 468 IHGLYWLYSVGIAGSHLVEGDNTIFLTQPRCTSPFQG--------IMYDYIRLE 475
           +             S L EG N + L+ P   + ++         + YD +RLE
Sbjct: 635 VSCYQVREKWEFPASWLKEGSNLLRLSLPTNGTNYESAVLPTSVYVQYDALRLE 658

BLAST of MC11g_new0113 vs. NCBI nr
Match: XP_038897922.1 (probable rhamnogalacturonate lyase B [Benincasa hispida])

HSP 1 Score: 961 bits (2485), Expect = 0.0
Identity = 442/478 (92.47%), Postives = 456/478 (95.40%), Query Frame = 0

Query: 1   MAVADNRQRFMPLPDDRLSGRCQTLAYPEAVLLTNPKNPQLKGEVDDKYQYSCENKDLKV 60
           MAVADNRQ+ MPLPDDRLSGRCQTLAYPEAVLLTNPKNP+LKGEVDDKYQYSCENKDLKV
Sbjct: 156 MAVADNRQKVMPLPDDRLSGRCQTLAYPEAVLLTNPKNPELKGEVDDKYQYSCENKDLKV 215

Query: 61  HGWICTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 120
           HGWICTNPPTGFW ITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG
Sbjct: 216 HGWICTNPPTGFWHITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 275

Query: 121 EPWKKVFGPVFLYFNSTSVGDDPFWLWEDAKIQMMTEVQSWPYSFPASEDFPKVDQRGTV 180
           EPWKKVFGPVF Y NSTSVGDDPFWLWEDAKIQMM EV+SWPYSFPASEDFPKVDQRG V
Sbjct: 276 EPWKKVFGPVFFYLNSTSVGDDPFWLWEDAKIQMMAEVESWPYSFPASEDFPKVDQRGVV 335

Query: 181 SGRLLVFDRYVSQDYLPASGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHIHA 240
            GRLLV DRYVSQDYLPASGAFVG+SAPGEVGSWQRECKGYQFWTRADDGGYFTINH H 
Sbjct: 336 RGRLLVLDRYVSQDYLPASGAFVGISAPGEVGSWQRECKGYQFWTRADDGGYFTINHAHT 395

Query: 241 GDYNLYAWVHGFIGDYRCDALININQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 300
           GDYNLY+WV GFIGDYRCDALIN+NQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY
Sbjct: 396 GDYNLYSWVPGFIGDYRCDALINVNQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 455

Query: 301 APDPNPIHINNLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKT 360
            PDPNP HIN LFVNHPDRFRQYGLWDRYAELYP+EDLVYTVGTSDYSKDWFFAQVPRKT
Sbjct: 456 VPDPNPTHINKLFVNHPDRFRQYGLWDRYAELYPNEDLVYTVGTSDYSKDWFFAQVPRKT 515

Query: 361 NSNAHQGTTWQIKFKLDSVDHKSMYMLRVAIASATLAELQVRINDPRSKRPLFTSGLIGR 420
            SN+H+GTTWQIKF L+ V  KSMY LRVAIASATLAELQVR+NDPRSKRPLFTSGLIGR
Sbjct: 516 KSNSHEGTTWQIKFYLNRVARKSMYTLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGR 575

Query: 421 DNSIARHGIHGLYWLYSVGIAGSHLVEGDNTIFLTQPRCTSPFQGIMYDYIRLEGPPT 478
           DNSIARHGIHG+YWLYSVG+ G HLVEG+NTIFLTQPRCTSPFQGIMYDYIRLEGPPT
Sbjct: 576 DNSIARHGIHGIYWLYSVGVGGCHLVEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPT 633

BLAST of MC11g_new0113 vs. NCBI nr
Match: XP_031739419.1 (probable rhamnogalacturonate lyase B isoform X2 [Cucumis sativus])

HSP 1 Score: 951 bits (2459), Expect = 0.0
Identity = 439/478 (91.84%), Postives = 454/478 (94.98%), Query Frame = 0

Query: 1   MAVADNRQRFMPLPDDRLSGRCQTLAYPEAVLLTNPKNPQLKGEVDDKYQYSCENKDLKV 60
           MAVADNRQR MPLPDDRLSGRCQTLAYPEAVLLTNPKNP LKGEVDDKYQYSCEN +LKV
Sbjct: 139 MAVADNRQRVMPLPDDRLSGRCQTLAYPEAVLLTNPKNPALKGEVDDKYQYSCENNNLKV 198

Query: 61  HGWICTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 120
           HGWI TNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLS HYAGQDLVPKFRAG
Sbjct: 199 HGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAG 258

Query: 121 EPWKKVFGPVFLYFNSTSVGDDPFWLWEDAKIQMMTEVQSWPYSFPASEDFPKVDQRGTV 180
           EPWKKVFGPVFLY NSTSVGDDPFWLWEDAKIQMM EV SWPYSFPASEDFPK DQRG V
Sbjct: 259 EPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDSWPYSFPASEDFPKKDQRGIV 318

Query: 181 SGRLLVFDRYVSQDYLPASGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHIHA 240
           SGRLLVFDRYV QDYLPA GAFVGLSAPGE GSWQRECKGYQFWTRADDGGYFTINH+H 
Sbjct: 319 SGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKGYQFWTRADDGGYFTINHVHT 378

Query: 241 GDYNLYAWVHGFIGDYRCDALININQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 300
           G+YNLYAWV GFIGDYRCDALIN++QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY
Sbjct: 379 GEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 438

Query: 301 APDPNPIHINNLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKT 360
            PDPNPIHIN LFV+HPDRFRQYGLW+RYAELYP+EDLVYTVGTSDY KDWFFA VPRKT
Sbjct: 439 VPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVYTVGTSDYRKDWFFAHVPRKT 498

Query: 361 NSNAHQGTTWQIKFKLDSVDHKSMYMLRVAIASATLAELQVRINDPRSKRPLFTSGLIGR 420
           N N+H+GTTWQIKFKLD VDHKSMY LRVAIASATLAELQVR+NDPRSKRPLFTSGLIGR
Sbjct: 499 N-NSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGR 558

Query: 421 DNSIARHGIHGLYWLYSVGIAGSHLVEGDNTIFLTQPRCTSPFQGIMYDYIRLEGPPT 478
           DNSIARHGIHG+YW+Y VG+AG HLVEG+NTIFLTQPRCTSPFQGIMYDYIRLEGPPT
Sbjct: 559 DNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPT 615

BLAST of MC11g_new0113 vs. NCBI nr
Match: XP_004136250.1 (probable rhamnogalacturonate lyase B isoform X1 [Cucumis sativus] >KGN60276.1 hypothetical protein Csa_001404 [Cucumis sativus])

HSP 1 Score: 951 bits (2459), Expect = 0.0
Identity = 439/478 (91.84%), Postives = 454/478 (94.98%), Query Frame = 0

Query: 1   MAVADNRQRFMPLPDDRLSGRCQTLAYPEAVLLTNPKNPQLKGEVDDKYQYSCENKDLKV 60
           MAVADNRQR MPLPDDRLSGRCQTLAYPEAVLLTNPKNP LKGEVDDKYQYSCEN +LKV
Sbjct: 156 MAVADNRQRVMPLPDDRLSGRCQTLAYPEAVLLTNPKNPALKGEVDDKYQYSCENNNLKV 215

Query: 61  HGWICTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 120
           HGWI TNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLS HYAGQDLVPKFRAG
Sbjct: 216 HGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAG 275

Query: 121 EPWKKVFGPVFLYFNSTSVGDDPFWLWEDAKIQMMTEVQSWPYSFPASEDFPKVDQRGTV 180
           EPWKKVFGPVFLY NSTSVGDDPFWLWEDAKIQMM EV SWPYSFPASEDFPK DQRG V
Sbjct: 276 EPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDSWPYSFPASEDFPKKDQRGIV 335

Query: 181 SGRLLVFDRYVSQDYLPASGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHIHA 240
           SGRLLVFDRYV QDYLPA GAFVGLSAPGE GSWQRECKGYQFWTRADDGGYFTINH+H 
Sbjct: 336 SGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKGYQFWTRADDGGYFTINHVHT 395

Query: 241 GDYNLYAWVHGFIGDYRCDALININQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 300
           G+YNLYAWV GFIGDYRCDALIN++QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY
Sbjct: 396 GEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 455

Query: 301 APDPNPIHINNLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKT 360
            PDPNPIHIN LFV+HPDRFRQYGLW+RYAELYP+EDLVYTVGTSDY KDWFFA VPRKT
Sbjct: 456 VPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVYTVGTSDYRKDWFFAHVPRKT 515

Query: 361 NSNAHQGTTWQIKFKLDSVDHKSMYMLRVAIASATLAELQVRINDPRSKRPLFTSGLIGR 420
           N N+H+GTTWQIKFKLD VDHKSMY LRVAIASATLAELQVR+NDPRSKRPLFTSGLIGR
Sbjct: 516 N-NSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGR 575

Query: 421 DNSIARHGIHGLYWLYSVGIAGSHLVEGDNTIFLTQPRCTSPFQGIMYDYIRLEGPPT 478
           DNSIARHGIHG+YW+Y VG+AG HLVEG+NTIFLTQPRCTSPFQGIMYDYIRLEGPPT
Sbjct: 576 DNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPT 632

BLAST of MC11g_new0113 vs. NCBI nr
Match: XP_008466137.1 (PREDICTED: probable rhamnogalacturonate lyase B isoform X1 [Cucumis melo] >KAA0038653.1 putative rhamnogalacturonate lyase B isoform X1 [Cucumis melo var. makuwa] >TYK31256.1 putative rhamnogalacturonate lyase B isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 951 bits (2458), Expect = 0.0
Identity = 439/478 (91.84%), Postives = 455/478 (95.19%), Query Frame = 0

Query: 1   MAVADNRQRFMPLPDDRLSGRCQTLAYPEAVLLTNPKNPQLKGEVDDKYQYSCENKDLKV 60
           MAVADNRQR MPLPDDRLSGRCQTLAYPEAVLLT+PKN  LKGEVDDKYQYSCEN +LKV
Sbjct: 156 MAVADNRQRVMPLPDDRLSGRCQTLAYPEAVLLTDPKNSALKGEVDDKYQYSCENNNLKV 215

Query: 61  HGWICTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 120
           HGWI TNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG
Sbjct: 216 HGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 275

Query: 121 EPWKKVFGPVFLYFNSTSVGDDPFWLWEDAKIQMMTEVQSWPYSFPASEDFPKVDQRGTV 180
           EPWKKVFGPVFLY NSTSVGDDPFWLWEDAKIQMM EV+SWPYSFPASEDF K DQRG V
Sbjct: 276 EPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVESWPYSFPASEDFSKKDQRGMV 335

Query: 181 SGRLLVFDRYVSQDYLPASGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHIHA 240
           SGRLLVFDRYV QDYLP SGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINH+  
Sbjct: 336 SGRLLVFDRYVDQDYLPGSGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHVLT 395

Query: 241 GDYNLYAWVHGFIGDYRCDALININQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 300
           GDYNLYAWV GFIGDYRCDAL+N++QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY
Sbjct: 396 GDYNLYAWVPGFIGDYRCDALVNVSQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 455

Query: 301 APDPNPIHINNLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKT 360
            PDPNPI+IN LFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDY KDWFFA VPRKT
Sbjct: 456 VPDPNPIYINKLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYRKDWFFAHVPRKT 515

Query: 361 NSNAHQGTTWQIKFKLDSVDHKSMYMLRVAIASATLAELQVRINDPRSKRPLFTSGLIGR 420
           NSN+H+GTTWQIKF+LD VD KSMYMLRVAIASATLAELQVR+NDPRSKRPLFTSGLIGR
Sbjct: 516 NSNSHEGTTWQIKFQLDRVDRKSMYMLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGR 575

Query: 421 DNSIARHGIHGLYWLYSVGIAGSHLVEGDNTIFLTQPRCTSPFQGIMYDYIRLEGPPT 478
           DNSIARHGIHG+YW+YSV +AG HLVEG+NTIFLTQPRCTSPFQGIMYDYIRLEGPPT
Sbjct: 576 DNSIARHGIHGIYWVYSVSVAGCHLVEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPT 633

BLAST of MC11g_new0113 vs. NCBI nr
Match: XP_008466138.1 (PREDICTED: probable rhamnogalacturonate lyase B isoform X2 [Cucumis melo])

HSP 1 Score: 951 bits (2458), Expect = 0.0
Identity = 439/478 (91.84%), Postives = 455/478 (95.19%), Query Frame = 0

Query: 1   MAVADNRQRFMPLPDDRLSGRCQTLAYPEAVLLTNPKNPQLKGEVDDKYQYSCENKDLKV 60
           MAVADNRQR MPLPDDRLSGRCQTLAYPEAVLLT+PKN  LKGEVDDKYQYSCEN +LKV
Sbjct: 62  MAVADNRQRVMPLPDDRLSGRCQTLAYPEAVLLTDPKNSALKGEVDDKYQYSCENNNLKV 121

Query: 61  HGWICTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 120
           HGWI TNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG
Sbjct: 122 HGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 181

Query: 121 EPWKKVFGPVFLYFNSTSVGDDPFWLWEDAKIQMMTEVQSWPYSFPASEDFPKVDQRGTV 180
           EPWKKVFGPVFLY NSTSVGDDPFWLWEDAKIQMM EV+SWPYSFPASEDF K DQRG V
Sbjct: 182 EPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVESWPYSFPASEDFSKKDQRGMV 241

Query: 181 SGRLLVFDRYVSQDYLPASGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHIHA 240
           SGRLLVFDRYV QDYLP SGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINH+  
Sbjct: 242 SGRLLVFDRYVDQDYLPGSGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHVLT 301

Query: 241 GDYNLYAWVHGFIGDYRCDALININQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 300
           GDYNLYAWV GFIGDYRCDAL+N++QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY
Sbjct: 302 GDYNLYAWVPGFIGDYRCDALVNVSQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 361

Query: 301 APDPNPIHINNLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKT 360
            PDPNPI+IN LFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDY KDWFFA VPRKT
Sbjct: 362 VPDPNPIYINKLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYRKDWFFAHVPRKT 421

Query: 361 NSNAHQGTTWQIKFKLDSVDHKSMYMLRVAIASATLAELQVRINDPRSKRPLFTSGLIGR 420
           NSN+H+GTTWQIKF+LD VD KSMYMLRVAIASATLAELQVR+NDPRSKRPLFTSGLIGR
Sbjct: 422 NSNSHEGTTWQIKFQLDRVDRKSMYMLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGR 481

Query: 421 DNSIARHGIHGLYWLYSVGIAGSHLVEGDNTIFLTQPRCTSPFQGIMYDYIRLEGPPT 478
           DNSIARHGIHG+YW+YSV +AG HLVEG+NTIFLTQPRCTSPFQGIMYDYIRLEGPPT
Sbjct: 482 DNSIARHGIHGIYWVYSVSVAGCHLVEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPT 539

BLAST of MC11g_new0113 vs. ExPASy TrEMBL
Match: A0A0A0LJQ9 (Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_3G892770 PE=3 SV=1)

HSP 1 Score: 951 bits (2459), Expect = 0.0
Identity = 439/478 (91.84%), Postives = 454/478 (94.98%), Query Frame = 0

Query: 1   MAVADNRQRFMPLPDDRLSGRCQTLAYPEAVLLTNPKNPQLKGEVDDKYQYSCENKDLKV 60
           MAVADNRQR MPLPDDRLSGRCQTLAYPEAVLLTNPKNP LKGEVDDKYQYSCEN +LKV
Sbjct: 156 MAVADNRQRVMPLPDDRLSGRCQTLAYPEAVLLTNPKNPALKGEVDDKYQYSCENNNLKV 215

Query: 61  HGWICTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 120
           HGWI TNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLS HYAGQDLVPKFRAG
Sbjct: 216 HGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRAG 275

Query: 121 EPWKKVFGPVFLYFNSTSVGDDPFWLWEDAKIQMMTEVQSWPYSFPASEDFPKVDQRGTV 180
           EPWKKVFGPVFLY NSTSVGDDPFWLWEDAKIQMM EV SWPYSFPASEDFPK DQRG V
Sbjct: 276 EPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVDSWPYSFPASEDFPKKDQRGIV 335

Query: 181 SGRLLVFDRYVSQDYLPASGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHIHA 240
           SGRLLVFDRYV QDYLPA GAFVGLSAPGE GSWQRECKGYQFWTRADDGGYFTINH+H 
Sbjct: 336 SGRLLVFDRYVDQDYLPACGAFVGLSAPGEAGSWQRECKGYQFWTRADDGGYFTINHVHT 395

Query: 241 GDYNLYAWVHGFIGDYRCDALININQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 300
           G+YNLYAWV GFIGDYRCDALIN++QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY
Sbjct: 396 GEYNLYAWVPGFIGDYRCDALINVSQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 455

Query: 301 APDPNPIHINNLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKT 360
            PDPNPIHIN LFV+HPDRFRQYGLW+RYAELYP+EDLVYTVGTSDY KDWFFA VPRKT
Sbjct: 456 VPDPNPIHINKLFVDHPDRFRQYGLWNRYAELYPNEDLVYTVGTSDYRKDWFFAHVPRKT 515

Query: 361 NSNAHQGTTWQIKFKLDSVDHKSMYMLRVAIASATLAELQVRINDPRSKRPLFTSGLIGR 420
           N N+H+GTTWQIKFKLD VDHKSMY LRVAIASATLAELQVR+NDPRSKRPLFTSGLIGR
Sbjct: 516 N-NSHEGTTWQIKFKLDRVDHKSMYTLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGR 575

Query: 421 DNSIARHGIHGLYWLYSVGIAGSHLVEGDNTIFLTQPRCTSPFQGIMYDYIRLEGPPT 478
           DNSIARHGIHG+YW+Y VG+AG HLVEG+NTIFLTQPRCTSPFQGIMYDYIRLEGPPT
Sbjct: 576 DNSIARHGIHGIYWVYGVGVAGCHLVEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPT 632

BLAST of MC11g_new0113 vs. ExPASy TrEMBL
Match: A0A5D3E6J1 (Rhamnogalacturonan endolyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005450 PE=3 SV=1)

HSP 1 Score: 951 bits (2458), Expect = 0.0
Identity = 439/478 (91.84%), Postives = 455/478 (95.19%), Query Frame = 0

Query: 1   MAVADNRQRFMPLPDDRLSGRCQTLAYPEAVLLTNPKNPQLKGEVDDKYQYSCENKDLKV 60
           MAVADNRQR MPLPDDRLSGRCQTLAYPEAVLLT+PKN  LKGEVDDKYQYSCEN +LKV
Sbjct: 156 MAVADNRQRVMPLPDDRLSGRCQTLAYPEAVLLTDPKNSALKGEVDDKYQYSCENNNLKV 215

Query: 61  HGWICTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 120
           HGWI TNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG
Sbjct: 216 HGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 275

Query: 121 EPWKKVFGPVFLYFNSTSVGDDPFWLWEDAKIQMMTEVQSWPYSFPASEDFPKVDQRGTV 180
           EPWKKVFGPVFLY NSTSVGDDPFWLWEDAKIQMM EV+SWPYSFPASEDF K DQRG V
Sbjct: 276 EPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVESWPYSFPASEDFSKKDQRGMV 335

Query: 181 SGRLLVFDRYVSQDYLPASGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHIHA 240
           SGRLLVFDRYV QDYLP SGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINH+  
Sbjct: 336 SGRLLVFDRYVDQDYLPGSGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHVLT 395

Query: 241 GDYNLYAWVHGFIGDYRCDALININQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 300
           GDYNLYAWV GFIGDYRCDAL+N++QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY
Sbjct: 396 GDYNLYAWVPGFIGDYRCDALVNVSQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 455

Query: 301 APDPNPIHINNLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKT 360
            PDPNPI+IN LFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDY KDWFFA VPRKT
Sbjct: 456 VPDPNPIYINKLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYRKDWFFAHVPRKT 515

Query: 361 NSNAHQGTTWQIKFKLDSVDHKSMYMLRVAIASATLAELQVRINDPRSKRPLFTSGLIGR 420
           NSN+H+GTTWQIKF+LD VD KSMYMLRVAIASATLAELQVR+NDPRSKRPLFTSGLIGR
Sbjct: 516 NSNSHEGTTWQIKFQLDRVDRKSMYMLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGR 575

Query: 421 DNSIARHGIHGLYWLYSVGIAGSHLVEGDNTIFLTQPRCTSPFQGIMYDYIRLEGPPT 478
           DNSIARHGIHG+YW+YSV +AG HLVEG+NTIFLTQPRCTSPFQGIMYDYIRLEGPPT
Sbjct: 576 DNSIARHGIHGIYWVYSVSVAGCHLVEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPT 633

BLAST of MC11g_new0113 vs. ExPASy TrEMBL
Match: A0A1S3CQJ4 (Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103503644 PE=3 SV=1)

HSP 1 Score: 951 bits (2458), Expect = 0.0
Identity = 439/478 (91.84%), Postives = 455/478 (95.19%), Query Frame = 0

Query: 1   MAVADNRQRFMPLPDDRLSGRCQTLAYPEAVLLTNPKNPQLKGEVDDKYQYSCENKDLKV 60
           MAVADNRQR MPLPDDRLSGRCQTLAYPEAVLLT+PKN  LKGEVDDKYQYSCEN +LKV
Sbjct: 156 MAVADNRQRVMPLPDDRLSGRCQTLAYPEAVLLTDPKNSALKGEVDDKYQYSCENNNLKV 215

Query: 61  HGWICTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 120
           HGWI TNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG
Sbjct: 216 HGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 275

Query: 121 EPWKKVFGPVFLYFNSTSVGDDPFWLWEDAKIQMMTEVQSWPYSFPASEDFPKVDQRGTV 180
           EPWKKVFGPVFLY NSTSVGDDPFWLWEDAKIQMM EV+SWPYSFPASEDF K DQRG V
Sbjct: 276 EPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVESWPYSFPASEDFSKKDQRGMV 335

Query: 181 SGRLLVFDRYVSQDYLPASGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHIHA 240
           SGRLLVFDRYV QDYLP SGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINH+  
Sbjct: 336 SGRLLVFDRYVDQDYLPGSGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHVLT 395

Query: 241 GDYNLYAWVHGFIGDYRCDALININQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 300
           GDYNLYAWV GFIGDYRCDAL+N++QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY
Sbjct: 396 GDYNLYAWVPGFIGDYRCDALVNVSQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 455

Query: 301 APDPNPIHINNLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKT 360
            PDPNPI+IN LFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDY KDWFFA VPRKT
Sbjct: 456 VPDPNPIYINKLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYRKDWFFAHVPRKT 515

Query: 361 NSNAHQGTTWQIKFKLDSVDHKSMYMLRVAIASATLAELQVRINDPRSKRPLFTSGLIGR 420
           NSN+H+GTTWQIKF+LD VD KSMYMLRVAIASATLAELQVR+NDPRSKRPLFTSGLIGR
Sbjct: 516 NSNSHEGTTWQIKFQLDRVDRKSMYMLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGR 575

Query: 421 DNSIARHGIHGLYWLYSVGIAGSHLVEGDNTIFLTQPRCTSPFQGIMYDYIRLEGPPT 478
           DNSIARHGIHG+YW+YSV +AG HLVEG+NTIFLTQPRCTSPFQGIMYDYIRLEGPPT
Sbjct: 576 DNSIARHGIHGIYWVYSVSVAGCHLVEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPT 633

BLAST of MC11g_new0113 vs. ExPASy TrEMBL
Match: A0A1S3CQI1 (Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103503644 PE=3 SV=1)

HSP 1 Score: 951 bits (2458), Expect = 0.0
Identity = 439/478 (91.84%), Postives = 455/478 (95.19%), Query Frame = 0

Query: 1   MAVADNRQRFMPLPDDRLSGRCQTLAYPEAVLLTNPKNPQLKGEVDDKYQYSCENKDLKV 60
           MAVADNRQR MPLPDDRLSGRCQTLAYPEAVLLT+PKN  LKGEVDDKYQYSCEN +LKV
Sbjct: 62  MAVADNRQRVMPLPDDRLSGRCQTLAYPEAVLLTDPKNSALKGEVDDKYQYSCENNNLKV 121

Query: 61  HGWICTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 120
           HGWI TNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG
Sbjct: 122 HGWISTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 181

Query: 121 EPWKKVFGPVFLYFNSTSVGDDPFWLWEDAKIQMMTEVQSWPYSFPASEDFPKVDQRGTV 180
           EPWKKVFGPVFLY NSTSVGDDPFWLWEDAKIQMM EV+SWPYSFPASEDF K DQRG V
Sbjct: 182 EPWKKVFGPVFLYLNSTSVGDDPFWLWEDAKIQMMAEVESWPYSFPASEDFSKKDQRGMV 241

Query: 181 SGRLLVFDRYVSQDYLPASGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHIHA 240
           SGRLLVFDRYV QDYLP SGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINH+  
Sbjct: 242 SGRLLVFDRYVDQDYLPGSGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHVLT 301

Query: 241 GDYNLYAWVHGFIGDYRCDALININQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 300
           GDYNLYAWV GFIGDYRCDAL+N++QGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY
Sbjct: 302 GDYNLYAWVPGFIGDYRCDALVNVSQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 361

Query: 301 APDPNPIHINNLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKT 360
            PDPNPI+IN LFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDY KDWFFA VPRKT
Sbjct: 362 VPDPNPIYINKLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYRKDWFFAHVPRKT 421

Query: 361 NSNAHQGTTWQIKFKLDSVDHKSMYMLRVAIASATLAELQVRINDPRSKRPLFTSGLIGR 420
           NSN+H+GTTWQIKF+LD VD KSMYMLRVAIASATLAELQVR+NDPRSKRPLFTSGLIGR
Sbjct: 422 NSNSHEGTTWQIKFQLDRVDRKSMYMLRVAIASATLAELQVRVNDPRSKRPLFTSGLIGR 481

Query: 421 DNSIARHGIHGLYWLYSVGIAGSHLVEGDNTIFLTQPRCTSPFQGIMYDYIRLEGPPT 478
           DNSIARHGIHG+YW+YSV +AG HLVEG+NTIFLTQPRCTSPFQGIMYDYIRLEGPPT
Sbjct: 482 DNSIARHGIHGIYWVYSVSVAGCHLVEGENTIFLTQPRCTSPFQGIMYDYIRLEGPPT 539

BLAST of MC11g_new0113 vs. ExPASy TrEMBL
Match: A0A6J1J0S6 (Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111480252 PE=3 SV=1)

HSP 1 Score: 926 bits (2392), Expect = 0.0
Identity = 426/478 (89.12%), Postives = 447/478 (93.51%), Query Frame = 0

Query: 1   MAVADNRQRFMPLPDDRLSGRCQTLAYPEAVLLTNPKNPQLKGEVDDKYQYSCENKDLKV 60
           MAVADNRQR MPLPDDRLSGRCQTL +PEAVLLTNPKNP+ + EVDDKYQYSCENKDLKV
Sbjct: 156 MAVADNRQRLMPLPDDRLSGRCQTLGFPEAVLLTNPKNPEHRDEVDDKYQYSCENKDLKV 215

Query: 61  HGWICTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 120
           HGWI  NPP GFWQITPSDEFRSGGP KQSLTSHVGPTTLAIFLS HYAGQDLVPKFR G
Sbjct: 216 HGWISNNPPIGFWQITPSDEFRSGGPHKQSLTSHVGPTTLAIFLSTHYAGQDLVPKFRNG 275

Query: 121 EPWKKVFGPVFLYFNSTSVGDDPFWLWEDAKIQMMTEVQSWPYSFPASEDFPKVDQRGTV 180
           EPWKKVFGPVFLY NST  GDDPFWLWEDAKIQMM EVQSWPYSFPAS++FPK+DQRGT+
Sbjct: 276 EPWKKVFGPVFLYLNSTKAGDDPFWLWEDAKIQMMAEVQSWPYSFPASDNFPKLDQRGTI 335

Query: 181 SGRLLVFDRYVSQDYLPASGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHIHA 240
           SGRLLVFDRY+S+DYLPASGAFVGLSAPGEVGSWQRECKGYQFWTRADD GYF INH+H 
Sbjct: 336 SGRLLVFDRYMSRDYLPASGAFVGLSAPGEVGSWQRECKGYQFWTRADDRGYFIINHVHI 395

Query: 241 GDYNLYAWVHGFIGDYRCDALININQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 300
           G YNLYAWV GFIGDYRCDALINI+QGS IE+G+LVYEPPRDGPTLWEIGIPDRSASEFY
Sbjct: 396 GQYNLYAWVPGFIGDYRCDALINISQGSSIELGNLVYEPPRDGPTLWEIGIPDRSASEFY 455

Query: 301 APDPNPIHINNLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKT 360
            PDPNP+HIN LFVNHP+RFRQYGLW+RYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKT
Sbjct: 456 IPDPNPMHINKLFVNHPERFRQYGLWERYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKT 515

Query: 361 NSNAHQGTTWQIKFKLDSVDHKSMYMLRVAIASATLAELQVRINDPRSKRPLFTSGLIGR 420
           N+N+HQGTTW+I FKLD VD KS YMLRVAIASATLAELQVR+NDP SKRPLFTSGLIGR
Sbjct: 516 NNNSHQGTTWKIAFKLDRVDRKSRYMLRVAIASATLAELQVRVNDPSSKRPLFTSGLIGR 575

Query: 421 DNSIARHGIHGLYWLYSVGIAGSHLVEGDNTIFLTQPRCTSPFQGIMYDYIRLEGPPT 478
           DNSIARHGIHG+YWLYSVGIAG HLVEGDN IF TQPRCTSPFQGIMYDYIRLEGP T
Sbjct: 576 DNSIARHGIHGIYWLYSVGIAGLHLVEGDNAIFFTQPRCTSPFQGIMYDYIRLEGPST 633

BLAST of MC11g_new0113 vs. TAIR 10
Match: AT1G09910.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 743.8 bits (1919), Expect = 8.6e-215
Identity = 328/478 (68.62%), Postives = 394/478 (82.43%), Query Frame = 0

Query: 1   MAVADNRQRFMPLPDDRLSGRCQTLAYPEAVLLTNPKNPQLKGEVDDKYQYSCENKDLKV 60
           MAVAD+R+R MP PDD   GRCQTL Y EA LLT P +P+L+GEVDDKYQYSCENKDL+V
Sbjct: 199 MAVADDRKRIMPFPDDLCKGRCQTLDYQEASLLTAPCDPRLQGEVDDKYQYSCENKDLRV 258

Query: 61  HGWICTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 120
           HGWI  +PP GFWQITPS+EFRSGGPLKQ+LTSHVGPTTLA+F S HYAG+ ++P+F  G
Sbjct: 259 HGWISFDPPVGFWQITPSNEFRSGGPLKQNLTSHVGPTTLAVFHSTHYAGKTMMPRFEHG 318

Query: 121 EPWKKVFGPVFLYFNSTSVGDDPFWLWEDAKIQMMTEVQSWPYSFPASEDFPKVDQRGTV 180
           EPWKKV+GPVF+Y NST+ GDDP  LW+DAKI+MM EV+ WPYSF AS+D+PK ++RGT 
Sbjct: 319 EPWKKVYGPVFIYLNSTANGDDPLCLWDDAKIKMMAEVERWPYSFVASDDYPKSEERGTA 378

Query: 181 SGRLLVFDRYVSQDYLPASGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHIHA 240
            GRLL+ DR+++ D + A GA+VGL+ PG+ GSWQ ECKGYQFW  AD+ GYF+I ++  
Sbjct: 379 RGRLLIRDRFINNDLISARGAYVGLAPPGDSGSWQIECKGYQFWAIADEAGYFSIGNVRP 438

Query: 241 GDYNLYAWVHGFIGDYRCDALININQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 300
           G+YNLYAWV  FIGDY    ++ +  G  IEMGD+VYEPPRDGPTLWEIGIPDR ASEF+
Sbjct: 439 GEYNLYAWVPSFIGDYHNGTIVRVTSGCMIEMGDIVYEPPRDGPTLWEIGIPDRKASEFF 498

Query: 301 APDPNPIHINNLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKT 360
            PDP+P  +N + V+H DRFRQYGLW +Y ++YP++DLVYTVG SDY +DWFFA VPRK 
Sbjct: 499 IPDPDPTLVNRVLVHHQDRFRQYGLWKKYTDMYPNDDLVYTVGVSDYRRDWFFAHVPRK- 558

Query: 361 NSNAHQGTTWQIKFKLDSVDHKSMYMLRVAIASATLAELQVRINDPRSKRPLFTSGLIGR 420
             + H+GTTWQI F L+++D K+ Y LRVAIASATLAELQ+RIND  + RPLFT+GLIGR
Sbjct: 559 KGDVHEGTTWQIIFNLENIDQKANYKLRVAIASATLAELQIRINDAEAIRPLFTTGLIGR 618

Query: 421 DNSIARHGIHGLYWLYSVGIAGSHLVEGDNTIFLTQPRCTSPFQGIMYDYIRLEGPPT 479
           DNSIARHGIHG+Y LY+V I G+ LV+GDNTIFL QPRC  PFQGIMYDYIRLEGPP+
Sbjct: 619 DNSIARHGIHGVYMLYAVNIPGNRLVQGDNTIFLKQPRCNGPFQGIMYDYIRLEGPPS 675

BLAST of MC11g_new0113 vs. TAIR 10
Match: AT1G09890.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 735.7 bits (1898), Expect = 2.3e-212
Identity = 333/478 (69.67%), Postives = 390/478 (81.59%), Query Frame = 0

Query: 1   MAVADNRQRFMPLPDDRLSGRCQTLAYPEAVLLTNPKNPQLKGEVDDKYQYSCENKDLKV 60
           MAV D+RQRFMPLPDDRL  R Q LAYPEAVLL NP   Q KGEVDDKYQYSCENKD+ V
Sbjct: 140 MAVTDDRQRFMPLPDDRLPDRGQALAYPEAVLLVNPLESQFKGEVDDKYQYSCENKDITV 199

Query: 61  HGWICTNPPT-GFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRA 120
           HGWICT  P+ GFW ITPS E+R+GGP KQ+LTSHVGPT LA+F+SAHY G+DLVPKF  
Sbjct: 200 HGWICTEQPSVGFWLITPSHEYRTGGPQKQNLTSHVGPTALAVFISAHYTGEDLVPKFSE 259

Query: 121 GEPWKKVFGPVFLYFNSTSVGD-DPFWLWEDAKIQMMTEVQSWPYSFPASEDFPKVDQRG 180
           GE WKKVFGPVF+Y NS++  D DP WLW+DAK QM  E +SWPYSFPAS+D+ K +QRG
Sbjct: 260 GEAWKKVFGPVFVYLNSSTDDDNDPLWLWQDAKSQMNVEAESWPYSFPASDDYVKTEQRG 319

Query: 181 TVSGRLLVFDRYVSQDYLPASGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHI 240
            V GRLLV DRYV +D++ A+  +VGL+ PG  GSWQRECK YQFWTR D+ G+F I+ I
Sbjct: 320 NVVGRLLVQDRYVDKDFIAANRGYVGLAVPGAAGSWQRECKEYQFWTRTDEEGFFYISGI 379

Query: 241 HAGDYNLYAWVHGFIGDYRCDALININQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASE 300
             G YNLYAW+ GFIGDY+ D +I I  G  I + DLVY+PPR+G TLWEIG PDRSA+E
Sbjct: 380 RPGQYNLYAWIPGFIGDYKYDDVITITSGCYIYVEDLVYQPPRNGATLWEIGFPDRSAAE 439

Query: 301 FYAPDPNPIHINNLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPR 360
           FY PDPNP +INNL+ NHPDRFRQYGLW+RYAELYPD+DLVY VG+SDY KDWF+AQV R
Sbjct: 440 FYVPDPNPKYINNLYQNHPDRFRQYGLWERYAELYPDKDLVYVVGSSDYRKDWFYAQVTR 499

Query: 361 KTNSNAHQGTTWQIKFKLDSVDHKSMYMLRVAIASATLAELQVRINDPRSKRPLFTSGLI 420
           K ++  +QGTTWQIKF+L ++D    Y LRVAIASAT +ELQ+R+N+  +  P+FTSGLI
Sbjct: 500 KKDNKTYQGTTWQIKFELKNIDKNHSYTLRVAIASATFSELQIRVNNANAS-PMFTSGLI 559

Query: 421 GRDNSIARHGIHGLYWLYSVGIAGSHLVEGDNTIFLTQPRCTSPFQGIMYDYIRLEGP 477
           GRDNSIARHGIHGLYWL++V +AGS L+EG+NT+FLTQPR TSPFQGIMYDYIR E P
Sbjct: 560 GRDNSIARHGIHGLYWLFNVEVAGSKLLEGENTLFLTQPRSTSPFQGIMYDYIRFEAP 616

BLAST of MC11g_new0113 vs. TAIR 10
Match: AT4G24430.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 686.0 bits (1769), Expect = 2.1e-197
Identity = 316/481 (65.70%), Postives = 381/481 (79.21%), Query Frame = 0

Query: 1   MAVADNRQRFMPLPDDRLSGRCQTLAYPEAVLLTNPKNPQLKGEVDDKYQYSCENKDLKV 60
           MA+ADNRQR MPLP+DRL  R + LAYPEAVLL +P   + KGEVDDKY+YS ENKDLKV
Sbjct: 160 MAIADNRQRKMPLPEDRLGKRGRPLAYPEAVLLVHPVEDEFKGEVDDKYEYSSENKDLKV 219

Query: 61  HGWICTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 120
           HGWI  N   G WQI PS+EFRSGG  KQ+LTSHVGP +LA+FLSAHYAG+D+V K +AG
Sbjct: 220 HGWISHNLDLGCWQIIPSNEFRSGGLSKQNLTSHVGPISLAMFLSAHYAGEDMVMKVKAG 279

Query: 121 EPWKKVFGPVFLYFNS-TSVGDDPFWLWEDAKIQMMTEVQSWPYSFPASEDFPKVDQRGT 180
           + WKKVFGPVF Y N       DP  LW+DAK QM+TEVQSWPY FPASEDFP  D+RG 
Sbjct: 280 DSWKKVFGPVFTYLNCLPDKTSDPLSLWQDAKNQMLTEVQSWPYDFPASEDFPVSDKRGC 339

Query: 181 VSGRLLVFDRYVSQDYLPASGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHIH 240
           +SGRLLV D+++S D+LPA+GAFVGL+ PGEVGSWQ E KGYQFWT AD  GYF IN I 
Sbjct: 340 ISGRLLVCDKFLSDDFLPANGAFVGLAPPGEVGSWQLESKGYQFWTEADSDGYFAINDIR 399

Query: 241 AGDYNLYAWVHGFIGDYRCDALININQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEF 300
            G+YNL  +V G+IGDY+ + LINI  G  I++G++VYEPPRDGPT+WEIGIPDRSA+EF
Sbjct: 400 EGEYNLNGYVTGWIGDYQYEQLINITAGCDIDVGNIVYEPPRDGPTVWEIGIPDRSAAEF 459

Query: 301 YAPDPNPIHINNLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRK 360
           + PDPNP +IN L++ HPDRFRQYGLW+RY ELYP EDLV+T+G SDY KDWFFA V RK
Sbjct: 460 FVPDPNPKYINKLYIGHPDRFRQYGLWERYTELYPKEDLVFTIGVSDYKKDWFFAHVTRK 519

Query: 361 TNSNAHQGTTWQIKFKLDSVDHKSMYMLRVAIASATLAELQVRINDPRSKR--PLFTSGL 420
              + +Q TTWQIKFKL++V     Y +R+A+A+A +AELQVR+ND  +++  P+FT+G+
Sbjct: 520 MGDDTYQKTTWQIKFKLENVQKSCTYKIRIALATANVAELQVRMNDDDTEKTTPIFTTGV 579

Query: 421 IGRDNSIARHGIHGLYWLYSVGIAGSHLVEGDNTIFLTQPRCTS-PFQGIMYDYIRLEGP 478
           IG DN+IARHGIHG+Y LY+V +    LVEGDNT+FLTQ   T+  F G+MYDYIRLEGP
Sbjct: 580 IGHDNAIARHGIHGIYRLYNVDVPSEKLVEGDNTLFLTQTMTTTGAFNGLMYDYIRLEGP 639

BLAST of MC11g_new0113 vs. TAIR 10
Match: AT1G09880.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 672.2 bits (1733), Expect = 3.2e-193
Identity = 301/481 (62.58%), Postives = 377/481 (78.38%), Query Frame = 0

Query: 1   MAVADNRQRFMPLPDDRLSGRCQTLAYPEAVLLTNPKNPQLKGEVDDKYQYSCENKDLKV 60
           MA++D+RQR+MP+PDDR+  R Q LAYPEAV L +P  P+ KGEVDDKY+YS E+KD+KV
Sbjct: 145 MAISDDRQRYMPVPDDRVPPRGQPLAYPEAVQLLDPIEPEFKGEVDDKYEYSMESKDIKV 204

Query: 61  HGWICTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 120
           HGWI TN   GFWQITPS+EFRS GPLKQ L SHVGPT LA+F S HY G DL+  F+ G
Sbjct: 205 HGWISTNDSVGFWQITPSNEFRSAGPLKQFLGSHVGPTNLAVFHSTHYVGADLIMSFKNG 264

Query: 121 EPWKKVFGPVFLYFNSTSVGDDPFWLWEDAKIQMMTEVQSWPYSFPASEDFPKVDQRGTV 180
           E WKKVFGPVF+Y NS   G DP  LW +AK Q   E + WPY+F AS+DFP  DQRG+V
Sbjct: 265 EAWKKVFGPVFIYLNSFPKGVDPLLLWHEAKNQTKIEEEKWPYNFTASDDFPASDQRGSV 324

Query: 181 SGRLLVFDRYVSQDYLPASGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHIHA 240
           SGRLLV DR++S + +PA+G++VGL+APG+VGSWQRECKGYQFW++AD+ G F+IN++ +
Sbjct: 325 SGRLLVRDRFISSEDIPANGSYVGLAAPGDVGSWQRECKGYQFWSKADENGSFSINNVRS 384

Query: 241 GDYNLYAWVHGFIGDYRCDALININQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 300
           G YNLYA+  GFIGDY  D + +I+ GS I +GDLVYEPPRDG TLWEIG+PDRSA+EFY
Sbjct: 385 GRYNLYAFAPGFIGDYHNDTVFDISPGSKISLGDLVYEPPRDGSTLWEIGVPDRSAAEFY 444

Query: 301 APDPNPIHINNLFVNHPDRFRQYGLWDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRKT 360
            PDPNP  +N L++NH D++RQYGLW+RY+ELYPDED+VY V   DYSK+WFF QV RK 
Sbjct: 445 IPDPNPSFVNKLYLNHSDKYRQYGLWERYSELYPDEDMVYNVDIDDYSKNWFFMQVTRKQ 504

Query: 361 NSNAHQGTTWQIKFKLDS--VDHKSMYMLRVAIASATLAELQVRINDPRSKRPLFTSGLI 420
            +  ++GTTWQI+F+ D    +    + LR+A+A++ +AELQVR+ND  +  PLF +  I
Sbjct: 505 ANGGYKGTTWQIRFQFDDKMKNVTGNFKLRIALATSNVAELQVRVNDLSADPPLFRTEQI 564

Query: 421 GRDNSIARHGIHGLYWLYSVGIAGSHLVEGDNTIFLTQPRCTSPFQGIMYDYIRLEGPPT 480
           GRDN+IARHGIHGLYWLYSV +  + L  G+NTI+LTQ   TSPFQG+MYDYIRLE P +
Sbjct: 565 GRDNTIARHGIHGLYWLYSVNVPAASLHVGNNTIYLTQALATSPFQGLMYDYIRLEYPDS 624

BLAST of MC11g_new0113 vs. TAIR 10
Match: AT2G22620.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 624.8 bits (1610), Expect = 5.8e-179
Identity = 294/477 (61.64%), Postives = 352/477 (73.79%), Query Frame = 0

Query: 1   MAVADNRQRFMPLPDDRLSGRCQTLAYPEAVLLTNPKNPQLKGEVDDKYQYSCENKDLKV 60
           MA++D+RQR MP   DR + +  +LAY EAVLLTNP NP  KGEVDDKY YS E+KD  V
Sbjct: 201 MAISDDRQRSMPSMADRENSK--SLAYKEAVLLTNPSNPMFKGEVDDKYMYSMEDKDNNV 260

Query: 61  HGWICTNPPTGFWQITPSDEFRSGGPLKQSLTSHVGPTTLAIFLSAHYAGQDLVPKFRAG 120
           HGWI ++PP GFW ITPSDEFR GGP+KQ LTSH GP TL++F S HYAG+++   +R G
Sbjct: 261 HGWISSDPPVGFWMITPSDEFRLGGPIKQDLTSHAGPITLSMFTSTHYAGKEMRMDYRNG 320

Query: 121 EPWKKVFGPVFLYFNSTSVGDDPFWLWEDAKIQMMTEVQSWPYSFPASEDFPKVDQRGTV 180
           EPWKKVFGPV  Y NS S  D    LW DAK QM  EV+SWPY F  SED+P   QRGT+
Sbjct: 321 EPWKKVFGPVLAYLNSVSPKDSTLRLWRDAKRQMAAEVKSWPYDFITSEDYPLRHQRGTL 380

Query: 181 SGRLLVFDRYVSQDYLPASGAFVGLSAPGEVGSWQRECKGYQFWTRADDGGYFTINHIHA 240
            G+ L+ D YVS+  +    AFVGL+  GE GSWQ E KGYQFWT+AD  G F I ++ A
Sbjct: 381 EGQFLIKDSYVSRLKIYGKFAFVGLAPIGEAGSWQTESKGYQFWTKADRRGRFIIENVRA 440

Query: 241 GDYNLYAWVHGFIGDYRCDALININQGSCIEMGDLVYEPPRDGPTLWEIGIPDRSASEFY 300
           G+Y+LYAW  GFIGDY+ +  I I  GS + +G LVYEPPR+GPTLWEIG+PDR+A EFY
Sbjct: 441 GNYSLYAWGSGFIGDYKYEQNITITPGSEMNVGPLVYEPPRNGPTLWEIGVPDRTAGEFY 500

Query: 301 APDPNPIHINNLFVNH-PDRFRQYGLWDRYAELYPDEDLVYTVGTSDYSKDWFFAQVPRK 360
            PDP P  +N L+VN   DRFRQYGLWDRYA+LYP  DLVYT+G SDY  DWFFA V R 
Sbjct: 501 IPDPYPTLMNKLYVNPLQDRFRQYGLWDRYADLYPQNDLVYTIGVSDYRSDWFFAHVARN 560

Query: 361 TNSNAHQGTTWQIKFKLDSVDHKSMYMLRVAIASATLAELQVRINDPRSKRPLFTSGLIG 420
             ++ +Q TTWQI F L +V+    Y LR+A+ASA  +ELQ+RINDP+S   +FT+G IG
Sbjct: 561 VGNDTYQPTTWQIIFNLKNVNRIGRYTLRIALASAADSELQIRINDPKSD-AIFTTGFIG 620

Query: 421 RDNSIARHGIHGLYWLYSVGIAGSHLVEGDNTIFLTQPRCTSPFQGIMYDYIRLEGP 477
           +DN+IARHGIHGLY LYS+ +AG+ L  GDNTIFLTQ R  +PFQGIMYDYIRLE P
Sbjct: 621 KDNAIARHGIHGLYRLYSIDVAGNLLSVGDNTIFLTQTRSRTPFQGIMYDYIRLESP 674