MC11g_new0104 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC11g_new0104
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionrust resistance kinase Lr10-like
LocationMC11: 3981173 .. 3981953 (+)
RNA-Seq ExpressionMC11g_new0104
SyntenyMC11g_new0104
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTGGTCGAAGATAGCCATCAATGGCGGGTCCTCTCAGCGGCCCATGGAGGAGAAATTACCATCTGCTCATGGTCGTGGGAGAGGGAGGAATCCGATGATACACACAGTAGGAGGAGACCGATTATTCTTGTACTCTCGAGGTCGGGCAACTTCGAATTCGAGGTTTCAATTATCAGTAGATTCATTATTAGGTATTTTCTTTTTTGTTGATCGTTAATATTATCTGTCGATGTCTTGGATTGAATCTGGTGTGGAAATGTCAGTGAGAAGGCCGAATTTTAAATTCATCCTGGAATTGACTCTGGAAGTAGCCATTGGGTGCACTGGTATTTGAGTACATGGAAAATGGATCTCTGGATAAGTATTTGTTTGGCAAAAAACAAGAAATTGAATGGGGAAAGTTACATGATGTAGCCATTGGGACTGCTAAAGGCCTTGCTTACTTACATGAGGAATGTCAACAGAGAATCATTCATTATGACATGAAGCCTGCTAACATTTTGTTGGATGCAAATTTCTCTCCCAAAGTTGGTGATTTTGGACTTGCCAAGCTTTGCAACAGGGATAGCACCCATGTATCTCTCACGGGCTATCGAGGCACTCCCGGATACTCCGCACCGGAGTTTCTATTGAACAACTATCCAATAACACACAAGTGCCATGTTTACAGCTTCGAAATGGTACTGTTTGAGATTGTTGGAAGAAAAAGGACTGCTGGTGTTACTAATTCTGGGAACCCGGATTGGTTTCCACAGCATGTGTGGGAGAGATATTAA

mRNA sequence

ATGGCCTGGTCGAAGATAGCCATCAATGGCGGGTCCTCTCAGCGGCCCATGGAGGAGAAATTACCATCTGCTCATGGTCGTGGGAGAGGGAGGAATCCGATGATACACACAGTAGGAGGAGACCGATTATTCTTGTACTCTCGAGGTCGGGCAACTTCGAATTCGAGGTTTCAATTATCAGTAGATTCATTATTAGTGAGAAGGCCGAATTTTAAATTCATCCTGGAATTGACTCTGGAAGTAGCCATTGGGTGCACTGCCATTGGGACTGCTAAAGGCCTTGCTTACTTACATGAGGAATGTCAACAGAGAATCATTCATTATGACATGAAGCCTGCTAACATTTTGTTGGATGCAAATTTCTCTCCCAAAGTTGGTGATTTTGGACTTGCCAAGCTTTGCAACAGGGATAGCACCCATGTATCTCTCACGGGCTATCGAGGCACTCCCGGATACTCCGCACCGGAGTTTCTATTGAACAACTATCCAATAACACACAAGTGCCATGTTTACAGCTTCGAAATGGTACTGTTTGAGATTGTTGGAAGAAAAAGGACTGCTGGTGTTACTAATTCTGGGAACCCGGATTGGTTTCCACAGCATGTGTGGGAGAGATATTAA

Coding sequence (CDS)

ATGGCCTGGTCGAAGATAGCCATCAATGGCGGGTCCTCTCAGCGGCCCATGGAGGAGAAATTACCATCTGCTCATGGTCGTGGGAGAGGGAGGAATCCGATGATACACACAGTAGGAGGAGACCGATTATTCTTGTACTCTCGAGGTCGGGCAACTTCGAATTCGAGGTTTCAATTATCAGTAGATTCATTATTAGTGAGAAGGCCGAATTTTAAATTCATCCTGGAATTGACTCTGGAAGTAGCCATTGGGTGCACTGCCATTGGGACTGCTAAAGGCCTTGCTTACTTACATGAGGAATGTCAACAGAGAATCATTCATTATGACATGAAGCCTGCTAACATTTTGTTGGATGCAAATTTCTCTCCCAAAGTTGGTGATTTTGGACTTGCCAAGCTTTGCAACAGGGATAGCACCCATGTATCTCTCACGGGCTATCGAGGCACTCCCGGATACTCCGCACCGGAGTTTCTATTGAACAACTATCCAATAACACACAAGTGCCATGTTTACAGCTTCGAAATGGTACTGTTTGAGATTGTTGGAAGAAAAAGGACTGCTGGTGTTACTAATTCTGGGAACCCGGATTGGTTTCCACAGCATGTGTGGGAGAGATATTAA

Protein sequence

MAWSKIAINGGSSQRPMEEKLPSAHGRGRGRNPMIHTVGGDRLFLYSRGRATSNSRFQLSVDSLLVRRPNFKFILELTLEVAIGCTAIGTAKGLAYLHEECQQRIIHYDMKPANILLDANFSPKVGDFGLAKLCNRDSTHVSLTGYRGTPGYSAPEFLLNNYPITHKCHVYSFEMVLFEIVGRKRTAGVTNSGNPDWFPQHVWERY
Homology
BLAST of MC11g_new0104 vs. ExPASy Swiss-Prot
Match: Q8RWZ5 (G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 OS=Arabidopsis thaliana OX=3702 GN=SD25 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.3e-28
Identity = 61/119 (51.26%), Postives = 81/119 (68.07%), Query Frame = 0

Query: 87  AIGTAKGLAYLHEECQQRIIHYDMKPANILLDANFSPKVGDFGLAKLCNRDSTHVSLTGY 146
           A+GTAKGLAYLHE+C  RI+H D+KP NILLD NF+ KV DFGLAKL  R+ +HV  T  
Sbjct: 596 ALGTAKGLAYLHEDCDARIVHCDIKPENILLDDNFNAKVSDFGLAKLMTREQSHV-FTTM 655

Query: 147 RGTPGYSAPEFLLNNYPITHKCHVYSFEMVLFEIVGRKRTAGVTNSGNPDWFPQHVWER 206
           RGT GY APE+ + NY I+ K  VYS+ MVL E++G ++    + +     FP   +++
Sbjct: 656 RGTRGYLAPEW-ITNYAISEKSDVYSYGMVLLELIGGRKNYDPSETSEKCHFPSFAFKK 712

BLAST of MC11g_new0104 vs. ExPASy Swiss-Prot
Match: P93604 (Rust resistance kinase Lr10 OS=Triticum aestivum OX=4565 GN=LRK10 PE=2 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 1.8e-28
Identity = 59/121 (48.76%), Postives = 85/121 (70.25%), Query Frame = 0

Query: 87  AIGTAKGLAYLHEECQQRIIHYDMKPANILLDANFSPKVGDFGLAKLCNRDSTHVSLTGY 146
           A+G A+G+ YLH+ C QRI+H+D+KP NILLD NF+PK+ DFGLAKLC RD + V+LT  
Sbjct: 444 ALGIARGMEYLHQGCNQRILHFDIKPHNILLDYNFNPKISDFGLAKLCARDQSIVTLTAA 503

Query: 147 RGTPGYSAPEFLLNNY-PITHKCHVYSFEMVLFEIV-GRKRTAGVTNSGNPDWFPQHVWE 206
           RGT GY APE    N+  +++K  VYSF M++ E+V GR+ +     S +  + P+ ++E
Sbjct: 504 RGTMGYIAPELYSRNFGGVSYKADVYSFGMLVLEMVSGRRNSDPRIGSQDDVYLPEWIYE 563

BLAST of MC11g_new0104 vs. ExPASy Swiss-Prot
Match: Q9XID3 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g34300 OS=Arabidopsis thaliana OX=3702 GN=At1g34300 PE=1 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 1.8e-28
Identity = 71/168 (42.26%), Postives = 92/168 (54.76%), Query Frame = 0

Query: 39  GGDRLFLYSRGRATSNSRFQLSVDSLLVRRPNFKFILELTLEVAIGCTAIGTAKGLAYLH 98
           G  RL +Y   R  S   F  + DS       ++F +           A+GTAKG+ YLH
Sbjct: 549 GRHRLLVYEFMRNGSLDNFLFTTDSAKFLTWEYRFNI-----------ALGTAKGITYLH 608

Query: 99  EECQQRIIHYDMKPANILLDANFSPKVGDFGLAKLCNRDSTHVSLTGYRGTPGYSAPEFL 158
           EEC+  I+H D+KP NIL+D NF+ KV DFGLAKL N      +++  RGT GY APE+L
Sbjct: 609 EECRDCIVHCDIKPENILVDDNFAAKVSDFGLAKLLNPKDNRYNMSSVRGTRGYLAPEWL 668

Query: 159 LNNYPITHKCHVYSFEMVLFEIVGRKRTAGVTNSGNPDWFPQHVWERY 207
             N PIT K  VYS+ MVL E+V  KR   V+   N   F    +E +
Sbjct: 669 A-NLPITSKSDVYSYGMVLLELVSGKRNFDVSEKTNHKKFSIWAYEEF 704

BLAST of MC11g_new0104 vs. ExPASy Swiss-Prot
Match: Q9FLV4 (G-type lectin S-receptor-like serine/threonine-protein kinase At5g24080 OS=Arabidopsis thaliana OX=3702 GN=At5g24080 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.5e-27
Identity = 60/113 (53.10%), Postives = 80/113 (70.80%), Query Frame = 0

Query: 87  AIGTAKGLAYLHEECQQRIIHYDMKPANILLDANFSPKVGDFGLAKLCNRDSTHVSLTGY 146
           A+ TA+G+AY HE+C+ RIIH D+KP NILLD NF PKV DFGLAK+  R+ +HV +T  
Sbjct: 634 AVATAQGIAYFHEQCRNRIIHCDIKPENILLDDNFCPKVSDFGLAKMMGREHSHV-VTMI 693

Query: 147 RGTPGYSAPEFLLNNYPITHKCHVYSFEMVLFEIVGRKRTAGVTNSGNPDWFP 200
           RGT GY APE+ ++N PIT K  VYS+ M+L EIVG +R   ++      ++P
Sbjct: 694 RGTRGYLAPEW-VSNRPITVKADVYSYGMLLLEIVGGRRNLDMSYDAEDFFYP 744

BLAST of MC11g_new0104 vs. ExPASy Swiss-Prot
Match: F4HQ23 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.7 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-2.7 PE=3 SV=3)

HSP 1 Score: 124.0 bits (310), Expect = 1.9e-27
Identity = 64/135 (47.41%), Postives = 90/135 (66.67%), Query Frame = 0

Query: 74  ILELTLEVAIGCTAIGTAKGLAYLHEECQQRIIHYDMKPANILLDANFSPKVGDFGLAKL 133
           IL+LT   A+   A+G A+GL YLH  C+ RI+H+D+KP N+LLD N SPKV DFGLAKL
Sbjct: 647 ILDLT---ALYGIALGVARGLEYLHYGCKTRIVHFDIKPQNVLLDDNLSPKVSDFGLAKL 706

Query: 134 CNRDSTHVSLTGYRGTPGYSAPEFLLNNY-PITHKCHVYSFEMVLFEIVG-RKRTAGVTN 193
           C +  + +SL   RGT GY APE +   Y  ++HK  VYS+ M++FE++G RK+     N
Sbjct: 707 CEKKESVMSLMDTRGTIGYIAPEMISRVYGSVSHKSDVYSYGMLVFEMIGARKKERFGQN 766

Query: 194 SGNPD--WFPQHVWE 205
           S N    +FP+ +++
Sbjct: 767 SANGSSMYFPEWIYK 778

BLAST of MC11g_new0104 vs. NCBI nr
Match: XP_022134951.1 (rust resistance kinase Lr10-like isoform X2 [Momordica charantia])

HSP 1 Score: 241 bits (614), Expect = 2.50e-74
Identity = 110/120 (91.67%), Postives = 115/120 (95.83%), Query Frame = 0

Query: 87  AIGTAKGLAYLHEECQQRIIHYDMKPANILLDANFSPKVGDFGLAKLCNRDSTHVSLTGY 146
           AIGTAKGLAYLHEECQQRIIHYD+KPANILLDANFSPKVGDFGLAKLCNRDSTH+SLTGY
Sbjct: 209 AIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHMSLTGY 268

Query: 147 RGTPGYSAPEFLLNNYPITHKCHVYSFEMVLFEIVGRKRTAGVTNSGNPDWFPQHVWERY 206
           RGTPGYSAPEFLL+N+PITHKC VYSF MVLFEI+GRKR AGVT SGNPDWFPQHVWE Y
Sbjct: 269 RGTPGYSAPEFLLSNFPITHKCDVYSFGMVLFEIIGRKRNAGVTPSGNPDWFPQHVWEEY 328

BLAST of MC11g_new0104 vs. NCBI nr
Match: XP_022134934.1 (rust resistance kinase Lr10-like isoform X1 [Momordica charantia] >XP_022134941.1 rust resistance kinase Lr10-like isoform X1 [Momordica charantia])

HSP 1 Score: 241 bits (614), Expect = 1.80e-73
Identity = 110/120 (91.67%), Postives = 115/120 (95.83%), Query Frame = 0

Query: 87  AIGTAKGLAYLHEECQQRIIHYDMKPANILLDANFSPKVGDFGLAKLCNRDSTHVSLTGY 146
           AIGTAKGLAYLHEECQQRIIHYD+KPANILLDANFSPKVGDFGLAKLCNRDSTH+SLTGY
Sbjct: 286 AIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHMSLTGY 345

Query: 147 RGTPGYSAPEFLLNNYPITHKCHVYSFEMVLFEIVGRKRTAGVTNSGNPDWFPQHVWERY 206
           RGTPGYSAPEFLL+N+PITHKC VYSF MVLFEI+GRKR AGVT SGNPDWFPQHVWE Y
Sbjct: 346 RGTPGYSAPEFLLSNFPITHKCDVYSFGMVLFEIIGRKRNAGVTPSGNPDWFPQHVWEEY 405

BLAST of MC11g_new0104 vs. NCBI nr
Match: XP_008466316.1 (PREDICTED: rust resistance kinase Lr10-like [Cucumis melo])

HSP 1 Score: 238 bits (606), Expect = 3.98e-73
Identity = 109/120 (90.83%), Postives = 113/120 (94.17%), Query Frame = 0

Query: 87  AIGTAKGLAYLHEECQQRIIHYDMKPANILLDANFSPKVGDFGLAKLCNRDSTHVSLTGY 146
           AIGTAKGLAYLHEECQQRIIHYD+KPANILLDANFSPKVGDFGLAKLCNRDSTH+SLTGY
Sbjct: 207 AIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHMSLTGY 266

Query: 147 RGTPGYSAPEFLLNNYPITHKCHVYSFEMVLFEIVGRKRTAGVTNSGNPDWFPQHVWERY 206
           RGTPGYSAPEFL  NYPITHKC VYSF MVLFEIVGRKR AGVT+SGNPDW PQHVW+ Y
Sbjct: 267 RGTPGYSAPEFLFYNYPITHKCDVYSFGMVLFEIVGRKRNAGVTDSGNPDWLPQHVWDNY 326

BLAST of MC11g_new0104 vs. NCBI nr
Match: XP_022982069.1 (rust resistance kinase Lr10-like isoform X2 [Cucurbita maxima] >XP_022982070.1 rust resistance kinase Lr10-like isoform X2 [Cucurbita maxima] >XP_022982071.1 rust resistance kinase Lr10-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 235 bits (599), Expect = 5.90e-73
Identity = 106/120 (88.33%), Postives = 113/120 (94.17%), Query Frame = 0

Query: 87  AIGTAKGLAYLHEECQQRIIHYDMKPANILLDANFSPKVGDFGLAKLCNRDSTHVSLTGY 146
           AIGTAKGLAYLHEECQQ++IHYD+KPANILLD NF+PKVGDFGLAKLCNRDSTH+SLTGY
Sbjct: 128 AIGTAKGLAYLHEECQQKVIHYDIKPANILLDENFTPKVGDFGLAKLCNRDSTHMSLTGY 187

Query: 147 RGTPGYSAPEFLLNNYPITHKCHVYSFEMVLFEIVGRKRTAGVTNSGNPDWFPQHVWERY 206
           RGTPGYSAPEFLL NYPITHKC VYSF MVLFEIVGRKR  GVT+SGNPDWFPQHVWE +
Sbjct: 188 RGTPGYSAPEFLLKNYPITHKCDVYSFGMVLFEIVGRKRNGGVTDSGNPDWFPQHVWEAH 247

BLAST of MC11g_new0104 vs. NCBI nr
Match: XP_022940212.1 (rust resistance kinase Lr10-like isoform X2 [Cucurbita moschata] >XP_022940213.1 rust resistance kinase Lr10-like isoform X2 [Cucurbita moschata] >XP_022940214.1 rust resistance kinase Lr10-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 234 bits (598), Expect = 8.36e-73
Identity = 107/118 (90.68%), Postives = 111/118 (94.07%), Query Frame = 0

Query: 87  AIGTAKGLAYLHEECQQRIIHYDMKPANILLDANFSPKVGDFGLAKLCNRDSTHVSLTGY 146
           AIGTAKGLAYLHEECQQ+IIHYD+KPANILLD NF+PKVGDFGLAKLCNRDSTH SLTGY
Sbjct: 128 AIGTAKGLAYLHEECQQKIIHYDIKPANILLDENFTPKVGDFGLAKLCNRDSTHTSLTGY 187

Query: 147 RGTPGYSAPEFLLNNYPITHKCHVYSFEMVLFEIVGRKRTAGVTNSGNPDWFPQHVWE 204
           RGTPGYSAPEFLL NYPITHKC VYSF MVLFEIVGRKR  GVT+SGNPDWFPQHVWE
Sbjct: 188 RGTPGYSAPEFLLKNYPITHKCDVYSFGMVLFEIVGRKRNGGVTDSGNPDWFPQHVWE 245

BLAST of MC11g_new0104 vs. ExPASy TrEMBL
Match: A0A6J1BZ80 (rust resistance kinase Lr10-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007065 PE=3 SV=1)

HSP 1 Score: 241 bits (614), Expect = 1.21e-74
Identity = 110/120 (91.67%), Postives = 115/120 (95.83%), Query Frame = 0

Query: 87  AIGTAKGLAYLHEECQQRIIHYDMKPANILLDANFSPKVGDFGLAKLCNRDSTHVSLTGY 146
           AIGTAKGLAYLHEECQQRIIHYD+KPANILLDANFSPKVGDFGLAKLCNRDSTH+SLTGY
Sbjct: 209 AIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHMSLTGY 268

Query: 147 RGTPGYSAPEFLLNNYPITHKCHVYSFEMVLFEIVGRKRTAGVTNSGNPDWFPQHVWERY 206
           RGTPGYSAPEFLL+N+PITHKC VYSF MVLFEI+GRKR AGVT SGNPDWFPQHVWE Y
Sbjct: 269 RGTPGYSAPEFLLSNFPITHKCDVYSFGMVLFEIIGRKRNAGVTPSGNPDWFPQHVWEEY 328

BLAST of MC11g_new0104 vs. ExPASy TrEMBL
Match: A0A6J1C045 (rust resistance kinase Lr10-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007065 PE=4 SV=1)

HSP 1 Score: 241 bits (614), Expect = 8.71e-74
Identity = 110/120 (91.67%), Postives = 115/120 (95.83%), Query Frame = 0

Query: 87  AIGTAKGLAYLHEECQQRIIHYDMKPANILLDANFSPKVGDFGLAKLCNRDSTHVSLTGY 146
           AIGTAKGLAYLHEECQQRIIHYD+KPANILLDANFSPKVGDFGLAKLCNRDSTH+SLTGY
Sbjct: 286 AIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHMSLTGY 345

Query: 147 RGTPGYSAPEFLLNNYPITHKCHVYSFEMVLFEIVGRKRTAGVTNSGNPDWFPQHVWERY 206
           RGTPGYSAPEFLL+N+PITHKC VYSF MVLFEI+GRKR AGVT SGNPDWFPQHVWE Y
Sbjct: 346 RGTPGYSAPEFLLSNFPITHKCDVYSFGMVLFEIIGRKRNAGVTPSGNPDWFPQHVWEEY 405

BLAST of MC11g_new0104 vs. ExPASy TrEMBL
Match: A0A1S3CR55 (rust resistance kinase Lr10-like OS=Cucumis melo OX=3656 GN=LOC103503762 PE=3 SV=1)

HSP 1 Score: 238 bits (606), Expect = 1.92e-73
Identity = 109/120 (90.83%), Postives = 113/120 (94.17%), Query Frame = 0

Query: 87  AIGTAKGLAYLHEECQQRIIHYDMKPANILLDANFSPKVGDFGLAKLCNRDSTHVSLTGY 146
           AIGTAKGLAYLHEECQQRIIHYD+KPANILLDANFSPKVGDFGLAKLCNRDSTH+SLTGY
Sbjct: 207 AIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHMSLTGY 266

Query: 147 RGTPGYSAPEFLLNNYPITHKCHVYSFEMVLFEIVGRKRTAGVTNSGNPDWFPQHVWERY 206
           RGTPGYSAPEFL  NYPITHKC VYSF MVLFEIVGRKR AGVT+SGNPDW PQHVW+ Y
Sbjct: 267 RGTPGYSAPEFLFYNYPITHKCDVYSFGMVLFEIVGRKRNAGVTDSGNPDWLPQHVWDNY 326

BLAST of MC11g_new0104 vs. ExPASy TrEMBL
Match: A0A6J1IYA8 (rust resistance kinase Lr10-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481026 PE=3 SV=1)

HSP 1 Score: 235 bits (599), Expect = 2.86e-73
Identity = 106/120 (88.33%), Postives = 113/120 (94.17%), Query Frame = 0

Query: 87  AIGTAKGLAYLHEECQQRIIHYDMKPANILLDANFSPKVGDFGLAKLCNRDSTHVSLTGY 146
           AIGTAKGLAYLHEECQQ++IHYD+KPANILLD NF+PKVGDFGLAKLCNRDSTH+SLTGY
Sbjct: 128 AIGTAKGLAYLHEECQQKVIHYDIKPANILLDENFTPKVGDFGLAKLCNRDSTHMSLTGY 187

Query: 147 RGTPGYSAPEFLLNNYPITHKCHVYSFEMVLFEIVGRKRTAGVTNSGNPDWFPQHVWERY 206
           RGTPGYSAPEFLL NYPITHKC VYSF MVLFEIVGRKR  GVT+SGNPDWFPQHVWE +
Sbjct: 188 RGTPGYSAPEFLLKNYPITHKCDVYSFGMVLFEIVGRKRNGGVTDSGNPDWFPQHVWEAH 247

BLAST of MC11g_new0104 vs. ExPASy TrEMBL
Match: A0A6J1FNN4 (rust resistance kinase Lr10-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445902 PE=3 SV=1)

HSP 1 Score: 234 bits (598), Expect = 4.05e-73
Identity = 107/118 (90.68%), Postives = 111/118 (94.07%), Query Frame = 0

Query: 87  AIGTAKGLAYLHEECQQRIIHYDMKPANILLDANFSPKVGDFGLAKLCNRDSTHVSLTGY 146
           AIGTAKGLAYLHEECQQ+IIHYD+KPANILLD NF+PKVGDFGLAKLCNRDSTH SLTGY
Sbjct: 128 AIGTAKGLAYLHEECQQKIIHYDIKPANILLDENFTPKVGDFGLAKLCNRDSTHTSLTGY 187

Query: 147 RGTPGYSAPEFLLNNYPITHKCHVYSFEMVLFEIVGRKRTAGVTNSGNPDWFPQHVWE 204
           RGTPGYSAPEFLL NYPITHKC VYSF MVLFEIVGRKR  GVT+SGNPDWFPQHVWE
Sbjct: 188 RGTPGYSAPEFLLKNYPITHKCDVYSFGMVLFEIVGRKRNGGVTDSGNPDWFPQHVWE 245

BLAST of MC11g_new0104 vs. TAIR 10
Match: AT4G32300.1 (S-domain-2 5 )

HSP 1 Score: 127.9 bits (320), Expect = 9.6e-30
Identity = 61/119 (51.26%), Postives = 81/119 (68.07%), Query Frame = 0

Query: 87  AIGTAKGLAYLHEECQQRIIHYDMKPANILLDANFSPKVGDFGLAKLCNRDSTHVSLTGY 146
           A+GTAKGLAYLHE+C  RI+H D+KP NILLD NF+ KV DFGLAKL  R+ +HV  T  
Sbjct: 596 ALGTAKGLAYLHEDCDARIVHCDIKPENILLDDNFNAKVSDFGLAKLMTREQSHV-FTTM 655

Query: 147 RGTPGYSAPEFLLNNYPITHKCHVYSFEMVLFEIVGRKRTAGVTNSGNPDWFPQHVWER 206
           RGT GY APE+ + NY I+ K  VYS+ MVL E++G ++    + +     FP   +++
Sbjct: 656 RGTRGYLAPEW-ITNYAISEKSDVYSYGMVLLELIGGRKNYDPSETSEKCHFPSFAFKK 712

BLAST of MC11g_new0104 vs. TAIR 10
Match: AT1G34300.1 (lectin protein kinase family protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.3e-29
Identity = 71/168 (42.26%), Postives = 92/168 (54.76%), Query Frame = 0

Query: 39  GGDRLFLYSRGRATSNSRFQLSVDSLLVRRPNFKFILELTLEVAIGCTAIGTAKGLAYLH 98
           G  RL +Y   R  S   F  + DS       ++F +           A+GTAKG+ YLH
Sbjct: 549 GRHRLLVYEFMRNGSLDNFLFTTDSAKFLTWEYRFNI-----------ALGTAKGITYLH 608

Query: 99  EECQQRIIHYDMKPANILLDANFSPKVGDFGLAKLCNRDSTHVSLTGYRGTPGYSAPEFL 158
           EEC+  I+H D+KP NIL+D NF+ KV DFGLAKL N      +++  RGT GY APE+L
Sbjct: 609 EECRDCIVHCDIKPENILVDDNFAAKVSDFGLAKLLNPKDNRYNMSSVRGTRGYLAPEWL 668

Query: 159 LNNYPITHKCHVYSFEMVLFEIVGRKRTAGVTNSGNPDWFPQHVWERY 207
             N PIT K  VYS+ MVL E+V  KR   V+   N   F    +E +
Sbjct: 669 A-NLPITSKSDVYSYGMVLLELVSGKRNFDVSEKTNHKKFSIWAYEEF 704

BLAST of MC11g_new0104 vs. TAIR 10
Match: AT5G24080.1 (Protein kinase superfamily protein )

HSP 1 Score: 124.4 bits (311), Expect = 1.1e-28
Identity = 60/113 (53.10%), Postives = 80/113 (70.80%), Query Frame = 0

Query: 87  AIGTAKGLAYLHEECQQRIIHYDMKPANILLDANFSPKVGDFGLAKLCNRDSTHVSLTGY 146
           A+ TA+G+AY HE+C+ RIIH D+KP NILLD NF PKV DFGLAK+  R+ +HV +T  
Sbjct: 232 AVATAQGIAYFHEQCRNRIIHCDIKPENILLDDNFCPKVSDFGLAKMMGREHSHV-VTMI 291

Query: 147 RGTPGYSAPEFLLNNYPITHKCHVYSFEMVLFEIVGRKRTAGVTNSGNPDWFP 200
           RGT GY APE+ ++N PIT K  VYS+ M+L EIVG +R   ++      ++P
Sbjct: 292 RGTRGYLAPEW-VSNRPITVKADVYSYGMLLLEIVGGRRNLDMSYDAEDFFYP 342

BLAST of MC11g_new0104 vs. TAIR 10
Match: AT1G66930.1 (Protein kinase superfamily protein )

HSP 1 Score: 124.0 bits (310), Expect = 1.4e-28
Identity = 64/135 (47.41%), Postives = 90/135 (66.67%), Query Frame = 0

Query: 74  ILELTLEVAIGCTAIGTAKGLAYLHEECQQRIIHYDMKPANILLDANFSPKVGDFGLAKL 133
           IL+LT   A+   A+G A+GL YLH  C+ RI+H+D+KP N+LLD N SPKV DFGLAKL
Sbjct: 438 ILDLT---ALYGIALGVARGLEYLHYGCKTRIVHFDIKPQNVLLDDNLSPKVSDFGLAKL 497

Query: 134 CNRDSTHVSLTGYRGTPGYSAPEFLLNNY-PITHKCHVYSFEMVLFEIVG-RKRTAGVTN 193
           C +  + +SL   RGT GY APE +   Y  ++HK  VYS+ M++FE++G RK+     N
Sbjct: 498 CEKKESVMSLMDTRGTIGYIAPEMISRVYGSVSHKSDVYSYGMLVFEMIGARKKERFGQN 557

Query: 194 SGNPD--WFPQHVWE 205
           S N    +FP+ +++
Sbjct: 558 SANGSSMYFPEWIYK 569

BLAST of MC11g_new0104 vs. TAIR 10
Match: AT1G66920.1 (Protein kinase superfamily protein )

HSP 1 Score: 122.1 bits (305), Expect = 5.3e-28
Identity = 57/122 (46.72%), Postives = 81/122 (66.39%), Query Frame = 0

Query: 87  AIGTAKGLAYLHEECQQRIIHYDMKPANILLDANFSPKVGDFGLAKLCNRDSTHVSLTGY 146
           A+G A+GL YLH  C+ RI+H+D+KP N+LLD N SPKV DFGLAKLC R  + +SL   
Sbjct: 401 ALGVARGLEYLHHGCRTRIVHFDIKPQNVLLDDNLSPKVSDFGLAKLCERKESILSLMDT 460

Query: 147 RGTPGYSAPEFLLNNY-PITHKCHVYSFEMVLFEIVGRKR---TAGVTNSGNPDWFPQHV 205
           RGT GY APE     Y  ++HK  VYS+ M++ +I+G +    T   T+S +  +FP+ +
Sbjct: 461 RGTIGYIAPEVFSRVYGSVSHKSDVYSYGMLVLDIIGARNKTSTEDTTSSTSSMYFPEWI 520

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RWZ51.3e-2851.26G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 OS=Arabidops... [more]
P936041.8e-2848.76Rust resistance kinase Lr10 OS=Triticum aestivum OX=4565 GN=LRK10 PE=2 SV=1[more]
Q9XID31.8e-2842.26G-type lectin S-receptor-like serine/threonine-protein kinase At1g34300 OS=Arabi... [more]
Q9FLV41.5e-2753.10G-type lectin S-receptor-like serine/threonine-protein kinase At5g24080 OS=Arabi... [more]
F4HQ231.9e-2747.41LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.7 OS=A... [more]
Match NameE-valueIdentityDescription
XP_022134951.12.50e-7491.67rust resistance kinase Lr10-like isoform X2 [Momordica charantia][more]
XP_022134934.11.80e-7391.67rust resistance kinase Lr10-like isoform X1 [Momordica charantia] >XP_022134941.... [more]
XP_008466316.13.98e-7390.83PREDICTED: rust resistance kinase Lr10-like [Cucumis melo][more]
XP_022982069.15.90e-7388.33rust resistance kinase Lr10-like isoform X2 [Cucurbita maxima] >XP_022982070.1 r... [more]
XP_022940212.18.36e-7390.68rust resistance kinase Lr10-like isoform X2 [Cucurbita moschata] >XP_022940213.1... [more]
Match NameE-valueIdentityDescription
A0A6J1BZ801.21e-7491.67rust resistance kinase Lr10-like isoform X2 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1C0458.71e-7491.67rust resistance kinase Lr10-like isoform X1 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A1S3CR551.92e-7390.83rust resistance kinase Lr10-like OS=Cucumis melo OX=3656 GN=LOC103503762 PE=3 SV... [more]
A0A6J1IYA82.86e-7388.33rust resistance kinase Lr10-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1FNN44.05e-7390.68rust resistance kinase Lr10-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT4G32300.19.6e-3051.26S-domain-2 5 [more]
AT1G34300.11.3e-2942.26lectin protein kinase family protein [more]
AT5G24080.11.1e-2853.10Protein kinase superfamily protein [more]
AT1G66930.11.4e-2847.41Protein kinase superfamily protein [more]
AT1G66920.15.3e-2846.72Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 68..205
e-value: 3.4E-33
score: 116.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availablePANTHERPTHR27009RUST RESISTANCE KINASE LR10-RELATEDcoord: 86..204
NoneNo IPR availablePANTHERPTHR27009:SF139PUTATIVE-RELATEDcoord: 86..204
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 88..185
e-value: 1.5E-21
score: 76.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 1..206
score: 20.767805
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 105..117
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 83..190

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC11g_new0104.1MC11g_new0104.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity