MC11g_new0046 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g_new0046
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	cytochrome P450 CYP82D47-like
Location	MC11: 1482316 .. 1483393 (-)
RNA-Seq Expression	MC11g_new0046
Synteny	MC11g_new0046

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCCGAATGCGTTGGGAGGAGAAGCAGAGGCAAAAGAGTTGCAGGTGGCGATTAGGGAGTCGTTCCGTTTGATGGGCCAAGGAATATTGAGGGACTTCATTCCTGTGGTTGCACAGTTGGGATTTGACGGACAAGTGAAGGCCATGGAGAAAATAGCCAAAAGAATTGATGCCATTCTTGAAGGATGGCTGCAGGACCACAAGAAAAGCAGAGCTTCTGGTTTGGCTCAAAAAAATGGTCGCTTTATGGATTCTCTCCTCTCCTTATATGATGGCAACGAGCTTCCAAGTCAGTACAATGGCTATATCATTACCAAAGCCACAACTTTGGTATTACCCCTTTTCTTTAATTATTATATTACCTTTTGTAGTACTCAACCAAATACTAATTATGTGTATATATGTGCAGAATGTGATTGCTGGTGGAACTGAAAGCAGCGCAATGACTCTAACATGGGCGATGTCATTACTGTTGAACAATCCGCATGCCCTGGGAAAGGCATATCAAGAGCTGGATGCGGTGGTGGGAAGAGAGATAGGCGAGTGAACGAGTCCGACATATTCAAGCTCAATTACCTACAGGCTATAATTAAGAAAACGATGCGACTATACCCAGCAAGGCCTCTATTGGGTCCCCATGAATTCTATAAGGATTGTGTCGTGGGTGGCTATTTTGTTCCCAAAGGTACTCAGCTGATTCCAAACATATGGAAGATCCAAACAGACCCACGAGTGTGGCCCGATCCGCTTAAATTCAAGCCGGAGCGGTTTCTAACGACCCACAAGGATGTGGATGTGAAAGGAAATGATTTCGAGTTGATTCCGTTTGGGAGCGGACAGAGAGGGTGTCCTGGAGTCGCATTTGGCCTTCAAATGGTGCACTTTGCTTTGGCTGGGTTTTTACAGTTTTTTGATGTGAAAAACCTAACGGGCAACACGATTGATATGTCGGAGGCTTTTGGAATGGCAAACGAGAAAATTTTGCCTCTCAATGTTATGGTCACCCCACGGTTGCCTTCTCCCCTTCATACCCTAATATAGTTATTTCGATATAGAAAGATTTATTATGTATGGCCAAG

mRNA sequence

ATGGGCGATGTCATTACTGTTGAACAATCCGCATGCCCTGGGAAAGGCATATCAAGAGCTGGATGCGGTGGTGGGAAGAGAGATAGGCGAGTGAACGAGTCCGACATATTCAAGCTCAATTACCTACAGGCTATAATTAAGAAAACGATGCGACTATACCCAGCAAGGCCTCTATTGGGTCCCCATGAATTCTATAAGGATTGTGTCGTGGGTGGCTATTTTGTTCCCAAAGGTACTCAGCTGATTCCAAACATATGGAAGATCCAAACAGACCCACGAGTGTGGCCCGATCCGCTTAAATTCAAGCCGGAGCGGTTTCTAACGACCCACAAGGATGTGGATGTGAAAGGAAATGATTTCGAGTTGATTCCGTTTGGGAGCGGACAGAGAGGGTGTCCTGGAGTCGCATTTGGCCTTCAAATGGTGCACTTTGCTTTGGCTGGGTTTTTACAGTTTTTTGATGTGAAAAACCTAACGGGCAACACGATTGATATGTCGGAGGCTTTTGGAATGGCAAACGAGAAAATTTTGCCTCTCAATGTTATGGTCACCCCACGGTTGCCTTCTCCCCTTCATACCCTAATATAG

Coding sequence (CDS)

ATGGGCGATGTCATTACTGTTGAACAATCCGCATGCCCTGGGAAAGGCATATCAAGAGCTGGATGCGGTGGTGGGAAGAGAGATAGGCGAGTGAACGAGTCCGACATATTCAAGCTCAATTACCTACAGGCTATAATTAAGAAAACGATGCGACTATACCCAGCAAGGCCTCTATTGGGTCCCCATGAATTCTATAAGGATTGTGTCGTGGGTGGCTATTTTGTTCCCAAAGGTACTCAGCTGATTCCAAACATATGGAAGATCCAAACAGACCCACGAGTGTGGCCCGATCCGCTTAAATTCAAGCCGGAGCGGTTTCTAACGACCCACAAGGATGTGGATGTGAAAGGAAATGATTTCGAGTTGATTCCGTTTGGGAGCGGACAGAGAGGGTGTCCTGGAGTCGCATTTGGCCTTCAAATGGTGCACTTTGCTTTGGCTGGGTTTTTACAGTTTTTTGATGTGAAAAACCTAACGGGCAACACGATTGATATGTCGGAGGCTTTTGGAATGGCAAACGAGAAAATTTTGCCTCTCAATGTTATGGTCACCCCACGGTTGCCTTCTCCCCTTCATACCCTAATATAG

Protein sequence

MGDVITVEQSACPGKGISRAGCGGGKRDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIWKIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFALAGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPLHTLI

Homology

[Close]

BLAST of MC11g_new0046 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 2.1e-55
Identity = 97/161 (60.25%), Postives = 122/161 (75.78%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           +DR + ESDI  L YLQA++K+T+RLY   PL  P EF +DC +GGY V KGT+LI NIW
Sbjct: 357 KDRCICESDISNLVYLQAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRLITNIW 416

Query: 87  KIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFAL 146
           KI TDP VW DP +FKP+RFLTTHKD+DVKG+ F+L+PFGSG+R CPG++FGLQ VH AL
Sbjct: 417 KIHTDPNVWSDPFEFKPDRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQTVHLAL 476

Query: 147 AGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRL 188
           A FL  F++ N +   +DM+EAFG+ N K  PL V+V P L
Sbjct: 477 ASFLHSFEILNPSTEPLDMTEAFGVTNTKATPLEVLVKPCL 517

[Close]

BLAST of MC11g_new0046 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 3.9e-54
Identity = 92/160 (57.50%), Postives = 119/160 (74.38%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           +D  + ESDI KL YLQAI+K+T+RLYP  P   P EF ++C++GGY + KGT+LI N+W
Sbjct: 359 KDEYIRESDISKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLW 418

Query: 87  KIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFAL 146
           KI  DP VW DPL+FKPERFLTTHKDVD++G++FEL+PFGSG+R C G++ GL MVHF L
Sbjct: 419 KIHRDPSVWSDPLEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTL 478

Query: 147 AGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPR 187
           A  L  FD+ N +   +DM+E FG  N K  PL ++V PR
Sbjct: 479 ANLLHSFDILNPSAEPVDMTEFFGFTNTKATPLEILVKPR 518

[Close]

BLAST of MC11g_new0046 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 209.1 bits (531), Expect = 4.4e-53
Identity = 93/161 (57.76%), Postives = 119/161 (73.91%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           ++R VNESDI KL YL AIIK+T+RLYP  P   P EF +DC +GGY + KGT+L+PN+W
Sbjct: 376 KERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFTEDCTIGGYHIKKGTRLMPNLW 435

Query: 87  KIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFAL 146
           KI  DP VWPDPL+FKPERFL+THKDVDV+G +FEL+PFGSG+R C G++ GL MVH+ L
Sbjct: 436 KIHRDPSVWPDPLEFKPERFLSTHKDVDVRGQNFELLPFGSGRRMCAGMSLGLHMVHYIL 495

Query: 147 AGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRL 188
           A FL  F++ N +  +ID++E       K  PL V+V P L
Sbjct: 496 ANFLHSFEILNPSPESIDVTEVLEFVTTKATPLEVLVKPCL 536

[Close]

BLAST of MC11g_new0046 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 6.5e-49
Identity = 91/166 (54.82%), Postives = 120/166 (72.29%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           RDR V +SDI  L YLQAIIK+T+RLYPA PLLGP E  +DC V GY+VP GT+LI N+W
Sbjct: 357 RDRNVEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVW 416

Query: 87  KIQTDPRVWPDPLKFKPERFLTTH-KDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFA 146
           KIQ DP+V+ +P +F+PERF+T   K+ DV+G +FEL+PFGSG+R CPG +  +Q++H  
Sbjct: 417 KIQRDPKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLG 476

Query: 147 LAGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPL 192
           LA FL  FDVK +    +DMSE  G+   K  PL V+++PR+   L
Sbjct: 477 LARFLHSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRIKEEL 522

[Close]

BLAST of MC11g_new0046 vs. ExPASy Swiss-Prot
Match: O81972 (Cytochrome P450 82A2 OS=Glycine max OX=3847 GN=CYP82A2 PE=2 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 1.5e-48
Identity = 86/161 (53.42%), Postives = 114/161 (70.81%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           ++R + ESD+ KL YLQA++K+T+RLYP  PL  P EF +DC +GGY V KGT+LI N+ 
Sbjct: 354 KERYICESDLSKLTYLQAVVKETLRLYPPAPLSRPREFEEDCTIGGYTVKKGTRLITNLS 413

Query: 87  KIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFAL 146
           KI TD  VW +PL+FKPERFLTT KD+D+KG  F+L+PFG G+R CPG+  GLQ V   L
Sbjct: 414 KIHTDHNVWSNPLEFKPERFLTTDKDIDMKGQHFQLLPFGGGRRICPGINLGLQTVRLTL 473

Query: 147 AGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRL 188
           A FL  F++ N +   +DM+E F   N K  PL +++ PRL
Sbjct: 474 ASFLHSFEILNPSTEPLDMTEVFRATNTKATPLEILIKPRL 514

[Close]

BLAST of MC11g_new0046 vs. NCBI nr
Match: XP_022137128.1 (cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 348 bits (894), Expect = 1.11e-116
Identity = 166/169 (98.22%), Postives = 167/169 (98.82%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           RDRRVNESDIFKLNYLQAIIK+TMRLYPA PLLGP EFYKDCVVGGYFVPKGTQLIPNIW
Sbjct: 277 RDRRVNESDIFKLNYLQAIIKETMRLYPAGPLLGPREFYKDCVVGGYFVPKGTQLIPNIW 336

Query: 87  KIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFAL 146
           KIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFAL
Sbjct: 337 KIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFAL 396

Query: 147 AGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPLHTLI 195
           AGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPLHTLI
Sbjct: 397 AGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPLHTLI 445

[Close]

BLAST of MC11g_new0046 vs. NCBI nr
Match: XP_022149464.1 (cytochrome P450 82A3-like [Momordica charantia])

HSP 1 Score: 318 bits (815), Expect = 1.31e-103
Identity = 151/169 (89.35%), Postives = 158/169 (93.49%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           RDRRVNESDI KL+YLQAI+K+TMRLYPA PLLGP EFYKDCVVGGYFVPKGTQLIPNIW
Sbjct: 364 RDRRVNESDISKLSYLQAIVKETMRLYPAGPLLGPREFYKDCVVGGYFVPKGTQLIPNIW 423

Query: 87  KIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFAL 146
           KIQ DPRVWPDPLKFKPERFLTTHK+VDVKGNDFELIPFGSG+RGCPGVAFGLQMVHF L
Sbjct: 424 KIQVDPRVWPDPLKFKPERFLTTHKEVDVKGNDFELIPFGSGRRGCPGVAFGLQMVHFTL 483

Query: 147 AGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPLHTLI 195
           AGFLQ FDVKN+T NTIDMSE FGMANEK LPLNV+VTPRL S LHTLI
Sbjct: 484 AGFLQSFDVKNVTSNTIDMSEVFGMANEKFLPLNVVVTPRLLSHLHTLI 532

[Close]

BLAST of MC11g_new0046 vs. NCBI nr
Match: KAA0032686.1 (cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa] >TYJ98719.1 cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa])

HSP 1 Score: 287 bits (734), Expect = 8.85e-96
Identity = 133/167 (79.64%), Postives = 149/167 (89.22%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           RDRR+ ESDI  L YLQ+IIK+TMR+YPA PLLGP EFYKDC+V GYFVPKGTQLIPN+W
Sbjct: 40  RDRRLKESDIPNLVYLQSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPNLW 99

Query: 87  KIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFAL 146
           +IQTDPRVWPDP KFKPERFLTTHK+VD+KGN+FELIPFGSG+RGCPG AFGLQMVHFAL
Sbjct: 100 RIQTDPRVWPDPFKFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGAAFGLQMVHFAL 159

Query: 147 AGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPLHT 193
           AGFL  FDV+N T   IDMSE FGMANEK++PLNV+VT RLPS L+T
Sbjct: 160 AGFLHSFDVRNPTKEPIDMSENFGMANEKVVPLNVLVTSRLPSHLYT 206

[Close]

BLAST of MC11g_new0046 vs. NCBI nr
Match: XP_022936278.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 293 bits (751), Expect = 5.29e-94
Identity = 136/166 (81.93%), Postives = 150/166 (90.36%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           RDRRVNESDI KL YLQA++K+TMRLYPA PLLGP EFYKDC V GYFVPKGTQLIPNIW
Sbjct: 361 RDRRVNESDITKLVYLQAVVKETMRLYPAGPLLGPREFYKDCFVAGYFVPKGTQLIPNIW 420

Query: 87  KIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFAL 146
           KIQTDPRVWPDP +FKPERFLTTH DVD+KGN+FELIPFGSG+RGCPG+AFGLQMVHFAL
Sbjct: 421 KIQTDPRVWPDPFEFKPERFLTTHCDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHFAL 480

Query: 147 AGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPLH 192
           AGFL  F+VK  T  +IDM+E FGMANEK++PLNV+VTPRLPS LH
Sbjct: 481 AGFLHSFNVKTPTPQSIDMAEGFGMANEKLVPLNVLVTPRLPSHLH 526

[Close]

BLAST of MC11g_new0046 vs. NCBI nr
Match: XP_022935740.1 (cytochrome P450 82A3-like [Cucurbita moschata])

HSP 1 Score: 288 bits (736), Expect = 8.99e-92
Identity = 136/166 (81.93%), Postives = 149/166 (89.76%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           R+RRV E D+ KL YLQAIIK+TMRLYPA PLLGP EF+KDCVVGGYFVPKGTQLIPNIW
Sbjct: 359 RERRVTEDDVGKLVYLQAIIKETMRLYPAGPLLGPREFHKDCVVGGYFVPKGTQLIPNIW 418

Query: 87  KIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFAL 146
           KIQTDPRVW +P +FKPERFLTTHKDVD+KGN FELIPFGSG+RGCPGVAFGLQM+HF L
Sbjct: 419 KIQTDPRVWCEPFEFKPERFLTTHKDVDLKGNHFELIPFGSGRRGCPGVAFGLQMMHFTL 478

Query: 147 AGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPLH 192
           AGFLQ FDVKN T + IDMSE FGMANEK+LPL+V+VT RLPS LH
Sbjct: 479 AGFLQCFDVKNPTKDPIDMSENFGMANEKVLPLHVLVTSRLPSHLH 524

[Close]

BLAST of MC11g_new0046 vs. ExPASy TrEMBL
Match: A0A6J1C9G6 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111008678 PE=3 SV=1)

HSP 1 Score: 348 bits (894), Expect = 5.36e-117
Identity = 166/169 (98.22%), Postives = 167/169 (98.82%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           RDRRVNESDIFKLNYLQAIIK+TMRLYPA PLLGP EFYKDCVVGGYFVPKGTQLIPNIW
Sbjct: 277 RDRRVNESDIFKLNYLQAIIKETMRLYPAGPLLGPREFYKDCVVGGYFVPKGTQLIPNIW 336

Query: 87  KIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFAL 146
           KIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFAL
Sbjct: 337 KIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFAL 396

Query: 147 AGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPLHTLI 195
           AGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPLHTLI
Sbjct: 397 AGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPLHTLI 445

[Close]

BLAST of MC11g_new0046 vs. ExPASy TrEMBL
Match: A0A6J1D746 (cytochrome P450 82A3-like OS=Momordica charantia OX=3673 GN=LOC111017857 PE=3 SV=1)

HSP 1 Score: 318 bits (815), Expect = 6.33e-104
Identity = 151/169 (89.35%), Postives = 158/169 (93.49%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           RDRRVNESDI KL+YLQAI+K+TMRLYPA PLLGP EFYKDCVVGGYFVPKGTQLIPNIW
Sbjct: 364 RDRRVNESDISKLSYLQAIVKETMRLYPAGPLLGPREFYKDCVVGGYFVPKGTQLIPNIW 423

Query: 87  KIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFAL 146
           KIQ DPRVWPDPLKFKPERFLTTHK+VDVKGNDFELIPFGSG+RGCPGVAFGLQMVHF L
Sbjct: 424 KIQVDPRVWPDPLKFKPERFLTTHKEVDVKGNDFELIPFGSGRRGCPGVAFGLQMVHFTL 483

Query: 147 AGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPLHTLI 195
           AGFLQ FDVKN+T NTIDMSE FGMANEK LPLNV+VTPRL S LHTLI
Sbjct: 484 AGFLQSFDVKNVTSNTIDMSEVFGMANEKFLPLNVVVTPRLLSHLHTLI 532

[Close]

BLAST of MC11g_new0046 vs. ExPASy TrEMBL
Match: A0A5D3BIA9 (Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold156G00110 PE=3 SV=1)

HSP 1 Score: 287 bits (734), Expect = 4.28e-96
Identity = 133/167 (79.64%), Postives = 149/167 (89.22%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           RDRR+ ESDI  L YLQ+IIK+TMR+YPA PLLGP EFYKDC+V GYFVPKGTQLIPN+W
Sbjct: 40  RDRRLKESDIPNLVYLQSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPNLW 99

Query: 87  KIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFAL 146
           +IQTDPRVWPDP KFKPERFLTTHK+VD+KGN+FELIPFGSG+RGCPG AFGLQMVHFAL
Sbjct: 100 RIQTDPRVWPDPFKFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGAAFGLQMVHFAL 159

Query: 147 AGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPLHT 193
           AGFL  FDV+N T   IDMSE FGMANEK++PLNV+VT RLPS L+T
Sbjct: 160 AGFLHSFDVRNPTKEPIDMSENFGMANEKVVPLNVLVTSRLPSHLYT 206

[Close]

BLAST of MC11g_new0046 vs. ExPASy TrEMBL
Match: A0A6J1FCU2 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442946 PE=3 SV=1)

HSP 1 Score: 293 bits (751), Expect = 2.56e-94
Identity = 136/166 (81.93%), Postives = 150/166 (90.36%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           RDRRVNESDI KL YLQA++K+TMRLYPA PLLGP EFYKDC V GYFVPKGTQLIPNIW
Sbjct: 361 RDRRVNESDITKLVYLQAVVKETMRLYPAGPLLGPREFYKDCFVAGYFVPKGTQLIPNIW 420

Query: 87  KIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFAL 146
           KIQTDPRVWPDP +FKPERFLTTH DVD+KGN+FELIPFGSG+RGCPG+AFGLQMVHFAL
Sbjct: 421 KIQTDPRVWPDPFEFKPERFLTTHCDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHFAL 480

Query: 147 AGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPLH 192
           AGFL  F+VK  T  +IDM+E FGMANEK++PLNV+VTPRLPS LH
Sbjct: 481 AGFLHSFNVKTPTPQSIDMAEGFGMANEKLVPLNVLVTPRLPSHLH 526

[Close]

BLAST of MC11g_new0046 vs. ExPASy TrEMBL
Match: A0A6J1FBI1 (cytochrome P450 82A3-like OS=Cucurbita moschata OX=3662 GN=LOC111442563 PE=3 SV=1)

HSP 1 Score: 288 bits (736), Expect = 4.35e-92
Identity = 136/166 (81.93%), Postives = 149/166 (89.76%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           R+RRV E D+ KL YLQAIIK+TMRLYPA PLLGP EF+KDCVVGGYFVPKGTQLIPNIW
Sbjct: 359 RERRVTEDDVGKLVYLQAIIKETMRLYPAGPLLGPREFHKDCVVGGYFVPKGTQLIPNIW 418

Query: 87  KIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFAL 146
           KIQTDPRVW +P +FKPERFLTTHKDVD+KGN FELIPFGSG+RGCPGVAFGLQM+HF L
Sbjct: 419 KIQTDPRVWCEPFEFKPERFLTTHKDVDLKGNHFELIPFGSGRRGCPGVAFGLQMMHFTL 478

Query: 147 AGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPLH 192
           AGFLQ FDVKN T + IDMSE FGMANEK+LPL+V+VT RLPS LH
Sbjct: 479 AGFLQCFDVKNPTKDPIDMSENFGMANEKVLPLHVLVTSRLPSHLH 524

[Close]

BLAST of MC11g_new0046 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 195.3 bits (495), Expect = 4.6e-50
Identity = 91/166 (54.82%), Postives = 120/166 (72.29%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           RDR V +SDI  L YLQAIIK+T+RLYPA PLLGP E  +DC V GY+VP GT+LI N+W
Sbjct: 357 RDRNVEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVW 416

Query: 87  KIQTDPRVWPDPLKFKPERFLTTH-KDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFA 146
           KIQ DP+V+ +P +F+PERF+T   K+ DV+G +FEL+PFGSG+R CPG +  +Q++H  
Sbjct: 417 KIQRDPKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLG 476

Query: 147 LAGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPL 192
           LA FL  FDVK +    +DMSE  G+   K  PL V+++PR+   L
Sbjct: 477 LARFLHSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRIKEEL 522

[Close]

BLAST of MC11g_new0046 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 193.4 bits (490), Expect = 1.8e-49
Identity = 92/166 (55.42%), Postives = 118/166 (71.08%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           RDR V +SDI  L YLQAIIK+T+RLYPA PLLG  E  +DC V GY VP GT+LI N+W
Sbjct: 345 RDRNVEDSDIKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVW 404

Query: 87  KIQTDPRVWPDPLKFKPERFLTTH-KDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFA 146
           KIQ DP+V+ +P +F+PERF+T   KD DV+G +FEL+PFGSG+R CPG +  +QM+H  
Sbjct: 405 KIQRDPKVYMEPNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLG 464

Query: 147 LAGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPL 192
           LA FL  F+VK +    +DMSE+ G+   K  PL V++ PRL   L
Sbjct: 465 LARFLHSFEVKTVLDRPVDMSESPGLTITKATPLEVLINPRLKREL 510

[Close]

BLAST of MC11g_new0046 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 189.9 bits (481), Expect = 1.9e-48
Identity = 87/167 (52.10%), Postives = 121/167 (72.46%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           RDR V +SDI  L Y+QAIIK+T+RLYPA PLLG  E  +DC V GY V +GT+++ N+W
Sbjct: 356 RDRNVEDSDIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVW 415

Query: 87  KIQTDPRVWPDPLKFKPERFLTTH-KDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFA 146
           KIQ DPRV+ +P +F+PERF+T   K+ DV+G +FEL+PFGSG+R CPG +  +Q++H  
Sbjct: 416 KIQRDPRVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLG 475

Query: 147 LAGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPLH 193
           LA FLQ FDVK +    +DM+E+ G+   K  PL ++++PRL   L+
Sbjct: 476 LARFLQSFDVKTVMDMPVDMTESPGLTIPKATPLEILISPRLKEGLY 522

[Close]

BLAST of MC11g_new0046 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 180.6 bits (457), Expect = 1.2e-45
Identity = 86/169 (50.89%), Postives = 115/169 (68.05%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           + R + ESDI  L YLQAI+K+T RLYP  PL G  E  +DC VGGY V KGT+L+ NIW
Sbjct: 349 KGRWIEESDIQNLKYLQAIVKETHRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIW 408

Query: 87  KIQTDPRVWPDPLKFKPERFLTTHKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHFAL 146
           K+  DP++WPDP  FKPERF+      +   ++FE IPFGSG+R CPGV  GL++VHF L
Sbjct: 409 KLHRDPKIWPDPKTFKPERFMEDKSQCE--KSNFEYIPFGSGRRSCPGVNLGLRVVHFVL 468

Query: 147 AGFLQFFDVKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPLHTLI 196
           A  LQ F++  ++   +DM+E  G+A  KI P+ V+V PRL   L++L+
Sbjct: 469 ARLLQGFELHKVSDEPLDMAEGPGLALPKINPVEVVVMPRLDPKLYSLL 515

[Close]

BLAST of MC11g_new0046 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 1.5e-40
Identity = 82/173 (47.40%), Postives = 110/173 (63.58%), Query Frame = 0


Query: 27  RDRRVNESDIFKLNYLQAIIKKTMRLYPARPLLGPHEFYKDCVVGGYFVPKGTQLIPNIW 86
           RDR VNESDI +L YLQA+IK+  RL+P  PL  PH   + C + GY +PKG+ L+ NIW
Sbjct: 338 RDRPVNESDIAQLPYLQAVIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIW 397

Query: 87  KIQTDPRVWPDPLKFKPERFLT--THKDVDVKGNDFELIPFGSGQRGCPGVAFGLQMVHF 146
            I  DP  W DPL FKPERFL       VDVKG+DFELIPFG+G+R C G++ GL+ + F
Sbjct: 398 AIARDPDQWSDPLAFKPERFLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQF 457

Query: 147 ALAGFLQFFD---VKNLTGNTIDMSEAFGMANEKILPLNVMVTPRLPSPLHTL 195
             A  +Q FD      +T   ++M E++G+  ++ +PL V   PRL   ++ L
Sbjct: 458 LTATLVQGFDWELAGGVTPEKLNMEESYGLTLQRAVPLVVHPKPRLAPNVYGL 510

The following BLAST results are available for this feature:

BLAST of MC11g_new0046 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49859	2.1e-55	60.25	Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1	[more]
O49858	3.9e-54	57.50	Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1	[more]
Q43068	4.4e-53	57.76	Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2	[more]
Q9SZ46	6.5e-49	54.82	Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=...	[more]
O81972	1.5e-48	53.42	Cytochrome P450 82A2 OS=Glycine max OX=3847 GN=CYP82A2 PE=2 SV=1	[more]

BLAST of MC11g_new0046 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137128.1	1.11e-116	98.22	cytochrome P450 CYP82D47-like [Momordica charantia]	[more]
XP_022149464.1	1.31e-103	89.35	cytochrome P450 82A3-like [Momordica charantia]	[more]
KAA0032686.1	8.85e-96	79.64	cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa] >TYJ98719.1 cytochrome ...	[more]
XP_022936278.1	5.29e-94	81.93	cytochrome P450 CYP82D47-like [Cucurbita moschata]	[more]
XP_022935740.1	8.99e-92	81.93	cytochrome P450 82A3-like [Cucurbita moschata]	[more]

BLAST of MC11g_new0046 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C9G6	5.36e-117	98.22	cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111008678 PE=...	[more]
A0A6J1D746	6.33e-104	89.35	cytochrome P450 82A3-like OS=Momordica charantia OX=3673 GN=LOC111017857 PE=3 SV...	[more]
A0A5D3BIA9	4.28e-96	79.64	Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A6J1FCU2	2.56e-94	81.93	cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442946 PE=3...	[more]
A0A6J1FBI1	4.35e-92	81.93	cytochrome P450 82A3-like OS=Cucurbita moschata OX=3662 GN=LOC111442563 PE=3 SV=...	[more]

BLAST of MC11g_new0046 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31940.1	4.6e-50	54.82	cytochrome P450, family 82, subfamily C, polypeptide 4	[more]
AT4G31950.1	1.8e-49	55.42	cytochrome P450, family 82, subfamily C, polypeptide 3	[more]
AT4G31970.1	1.9e-48	52.10	cytochrome P450, family 82, subfamily C, polypeptide 2	[more]
AT3G25180.1	1.2e-45	50.89	cytochrome P450, family 82, subfamily G, polypeptide 1	[more]
AT5G07990.1	1.5e-40	47.40	Cytochrome P450 superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 43..61 score: 32.54 coord: 132..155 score: 26.34 coord: 84..108 score: 40.5 coord: 122..132 score: 51.01
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 27..168 e-value: 7.7E-41 score: 140.3
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 17..181 e-value: 3.7E-47 score: 162.9
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 27..186
None	No IPR available	PANTHER	PTHR47947:SF26	CYTOCHROME P450	coord: 27..190
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 27..190
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 125..134

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g_new0046.1	MC11g_new0046.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098542	defense response to other organism
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen