Homology
BLAST of MC11g_new0043 vs. ExPASy Swiss-Prot
Match:
Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)
HSP 1 Score: 508.8 bits (1309), Expect = 7.1e-143
Identity = 257/518 (49.61%), Postives = 355/518 (68.53%), Query Frame = 0
Query: 13 LLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGKEP-AHATLGRMADAHGPI 72
L+F+F ALF S + + P G WP+IGHLHLL GKE + TLG+MAD +GP
Sbjct: 13 LVFVFIALFKKSKK---PKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPA 72
Query: 73 FMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLGYEYAMFGTSPYGSHWKH 132
L+ G + A VVS +E++++CFT NDK ASRP AAAK +GY +A+FG +PY + W+
Sbjct: 73 MSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWRE 132
Query: 133 VPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGGGGKALVEMTTWFGDITL 192
+ KI T+E+L+N RL+ L+H+R SE+ +K L L N GG +V++ +W D+TL
Sbjct: 133 MRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKN-GGTKPVMVDLKSWLEDMTL 192
Query: 193 NTIFRIVVGKRFSTAFEGSG---------GEQYHKALSDFFDLFGAFIPSDSFPFLSWLD 252
N I R+V GKR+ F G G Q KA++ FF L G F SD+FP LS+ D
Sbjct: 193 NMIVRMVAGKRY---FGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFD 252
Query: 253 LGGYEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKDDELS--GY 312
L G+EK M+ T LD +L++W++ HR++ F G + + DF+DVM+S + +LS Y
Sbjct: 253 LQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQGKLSHLQY 312
Query: 313 DVDTVAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRERQVNESD 372
D +T K+TCLALILGG T++ +TWA+SLLLNN+E LKK Q E+D VGR+R V +SD
Sbjct: 313 DANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSD 372
Query: 373 IKNLIYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQRDPLVW 432
I+NL+YLQA++KETLRLY A PL P E++EDCTVAGY++P GTRL VN+ K+QRDP V+
Sbjct: 373 IENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVY 432
Query: 433 ENPGEFCPERFITSQ-KNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKLLHGFE 492
P EF PERFIT + K FDV+GQN +L+PFG+GRR CPG S A+QV+HL LA+ LH F+
Sbjct: 433 MEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFD 492
Query: 493 IKRPSEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIY 518
+K + P++M E+ GL I + TPLEV++S R+ +++
Sbjct: 493 VKTVMDMPVDMSENPGLTIPKATPLEVLISPRIKEELF 523
BLAST of MC11g_new0043 vs. ExPASy Swiss-Prot
Match:
O49394 (Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=1 SV=2)
HSP 1 Score: 486.5 bits (1251), Expect = 3.8e-136
Identity = 246/514 (47.86%), Postives = 345/514 (67.12%), Query Frame = 0
Query: 13 LLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGKEP-AHATLGRMADAHGPI 72
L+F+F ALF S + + P G WP+IGHLHLLSGKE + TLG+MAD +GP
Sbjct: 13 LVFVFIALFKKSKK---PKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQYGPA 72
Query: 73 FMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLGYEYAMFGTSPYGSHWKH 132
LR G VVS +E++++CFT NDK ASRP AAAK +GY+ A+FG +PY + W+
Sbjct: 73 MSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWRE 132
Query: 133 VPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGGGGKALVEMTTWFGDITL 192
+ KI TLE+L+N RL+ L+H+R SE+ ++ L L V +GG +V++ +W D++L
Sbjct: 133 MRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWV-KKGGSEPVMVDLKSWLEDMSL 192
Query: 193 NTIFRIVVGKRF-----STAFEGSGGEQYHKALSDFFDLFGAFIPSDSFPFLSWLDLGGY 252
N + R+V GKR+ + + Q K +++FF L G F SD+FP L W D G+
Sbjct: 193 NMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFDFQGH 252
Query: 253 EKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKDDELS--GYDVDT 312
EK M+ T + LD +L++W++ HR++ G + + DF+DVMLS + + S +D T
Sbjct: 253 EKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKFSHLQHDAIT 312
Query: 313 VAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRERQVNESDIKNL 372
K+TCLALILGG T+ +TWA+SLLLNN++ LKK Q E+D VGR+R V +SDI+NL
Sbjct: 313 SIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENL 372
Query: 373 IYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQRDPLVWENPG 432
+Y+QA++KETLRLY A PL E+IEDCTVAGY++ GTR+ VN+ K+QRDP V+ P
Sbjct: 373 VYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPN 432
Query: 433 EFCPERFITSQ-KNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKLLHGFEIKRP 492
EF PERFIT + K FDV+GQN +L+PFG+GRR CPG S A+QV+HL LA+ L F++K
Sbjct: 433 EFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKTV 492
Query: 493 SEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIY 518
+ P++M ES GL I + TPLE+++S RL +Y
Sbjct: 493 MDMPVDMTESPGLTIPKATPLEILISPRLKEGLY 522
BLAST of MC11g_new0043 vs. ExPASy Swiss-Prot
Match:
A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)
HSP 1 Score: 481.5 bits (1238), Expect = 1.2e-134
Identity = 245/514 (47.67%), Postives = 338/514 (65.76%), Query Frame = 0
Query: 13 LLFLFYALFTISSRFGTRRKN----LPPEAGGGWPVIGHLHLLSGKEPAHATLGRMADAH 72
LL F+ L F ++ N PP+ G WP+IGHLHLL+ + H L AD +
Sbjct: 10 LLLGFFVLLPCFFYFVWKKPNNKIKEPPQPAGAWPIIGHLHLLARGDLPHKILSSFADKN 69
Query: 73 GPIFMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLGYEYAMFGTSPYGSH 132
GP+F ++ G+H+ALVV+ EI++ECFTTND+ F +RP AAK++GY Y M G +PYG +
Sbjct: 70 GPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVMLGVAPYGPY 129
Query: 133 WKHVPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGGGGKALVEMTTWFGD 192
W+ + KI+ LE L+N RL+ L+H+ SE+ S K L +L LV+M W D
Sbjct: 130 WRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLW--ETQNIDFCLVDMKQWLAD 189
Query: 193 ITLNTIFRIVVGKRF-----STAFEGSGGEQYHKALSDFFDLFGAFIPSDSFPFLSWLDL 252
+TLN ++VVGKRF ++A E + K L + F L G+F+ SD P+L WLDL
Sbjct: 190 LTLNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYLPYLRWLDL 249
Query: 253 GGYEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKDDELSGYDVD 312
GG+EK M+ T K LD + WL EH+ + G E ++ DFMDVMLS +++ +L G DVD
Sbjct: 250 GGHEKEMKRTVKELDILFKGWLDEHKRKRLSGGKEDDDQDFMDVMLSILEESKL-GNDVD 309
Query: 313 TVAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRERQVNESDIKN 372
T+ K CLA+ILGG TT +TWALSLLLNN ALKK Q ELD VGR+R V+ESD+
Sbjct: 310 TINKTACLAIILGGADTTWATLTWALSLLLNNPNALKKAQDELDLHVGRDRNVDESDLVK 369
Query: 373 LIYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQRDPLVWENP 432
L Y+ A++KETLRLY PL P EDCT+AGYH+ GTRL VN K+QRDPLVW P
Sbjct: 370 LTYIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVNAWKIQRDPLVWSQP 429
Query: 433 GEFCPERFITSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKLLHGFEIKRP 492
E+ PERF+ +++ D+KGQ+ +LIPFG+GRR CP +S A+QV+ LTLA +LHGFE++ P
Sbjct: 430 HEYQPERFL--ERDVDMKGQHFELIPFGSGRRACPAISLALQVLPLTLAHILHGFELRTP 489
Query: 493 SEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIY 518
++ ++M E+ G+ + TPLEV+++ R+S + +
Sbjct: 490 NQNKVDMTETPGIVHAKATPLEVLVAPRISPKCF 518
BLAST of MC11g_new0043 vs. ExPASy Swiss-Prot
Match:
O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)
HSP 1 Score: 479.9 bits (1234), Expect = 3.5e-134
Identity = 239/515 (46.41%), Postives = 336/515 (65.24%), Query Frame = 0
Query: 8 AAILALLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGKEPAHATLGRMADA 67
A+IL+L+FL L+ R +R K+ P G WP++GHL LL+G + H LG +AD
Sbjct: 16 ASILSLIFLCLFLY----RKNSRGKD-APVVSGAWPILGHLSLLNGSQTPHKVLGALADK 75
Query: 68 HGPIFMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLGYEYAMFGTSPYGS 127
+GP+F ++ GM ALV+S WE+S+E FTTND +SRPK+ A +++ Y A G +PYG
Sbjct: 76 YGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFVGLAPYGP 135
Query: 128 HWKHVPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVI-NEGGGGKALVEMTTWF 187
+W+ + KIVT E L+N R+E+ HIR SEV +SIK L ++ N+ LV++ W
Sbjct: 136 YWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYTLVDIKQWL 195
Query: 188 GDITLNTIFRIVVGKRFSTAFEGSG---GEQYHKALSDFFDLFGAFIPSDSFPFLSWLDL 247
+T N + R+VVGKR+ G +++ K + +F +L G F +D P L WLDL
Sbjct: 196 AYLTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGVPCLRWLDL 255
Query: 248 GGYEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKDDELSGYDVD 307
GG+EKAM+ AK +D++L +WL+EHR++ + DFMDVM+S + ++ +D D
Sbjct: 256 GGHEKAMKANAKEVDKLLSEWLEEHRQKKLLGENVESDRDFMDVMISALNGAQIGAFDAD 315
Query: 308 TVAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRERQVNESDIKN 367
T+ KAT L LILGG +T+V +TWALSLLL N AL K + E+D Q+G++ + ESDI
Sbjct: 316 TICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGKDEYIRESDISK 375
Query: 368 LIYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQRDPLVWENP 427
L+YLQA+VKETLRLY AP S P E E+C + GYHI GTRL NL K+ RDP VW +P
Sbjct: 376 LVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWKIHRDPSVWSDP 435
Query: 428 GEFCPERFITSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKLLHGFEIKRP 487
EF PERF+T+ K+ D++G N +L+PFG+GRR+C GMS + ++H TLA LLH F+I P
Sbjct: 436 LEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTLANLLHSFDILNP 495
Query: 488 SEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIYE 519
S EP++M E G T+ TPLE+++ R S YE
Sbjct: 496 SAEPVDMTEFFGFTNTKATPLEILVKPRQSPNYYE 525
BLAST of MC11g_new0043 vs. ExPASy Swiss-Prot
Match:
O49396 (Cytochrome P450 82C3 OS=Arabidopsis thaliana OX=3702 GN=CYP82C3 PE=2 SV=3)
HSP 1 Score: 472.6 bits (1215), Expect = 5.6e-132
Identity = 250/524 (47.71%), Postives = 342/524 (65.27%), Query Frame = 0
Query: 7 SAAILALLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGKEP-AHATLGRMA 66
S + L+F+F ALF S + + P G WP+IGHLHLL GKE + TLG+MA
Sbjct: 7 SLFVSILVFVFIALFKKSKK---PKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMA 66
Query: 67 DAHGPIFMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLGYEYAMFGTSPY 126
D +GP LR G V S +E++++CFT NDK AS AAAK +GY +
Sbjct: 67 DHYGPAMSLRLGSSETFVGSSFEVAKDCFTVNDKALASL-MTAAAKHMGYVF-------- 126
Query: 127 GSHWKHVPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGGGGKALVEMTTW 186
W + KI +E+L+N RL+ L ++R SE+ +K L L V +GG +V++ +W
Sbjct: 127 ---WLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWV-KKGGSEPVMVDLKSW 186
Query: 187 FGDITLNTIFRIVVGKRFSTAFEGSGGE---------QYHKALSDFFDLFGAFIPSDSFP 246
D+ N I R+V GKR+ F G G E Q+ K ++ FF L G F SD+FP
Sbjct: 187 LEDMIANMIMRMVAGKRY---FGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFP 246
Query: 247 FLSWLDLGGYEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKDDE 306
L WLDL G+EK M+ T + LD +L++W++ HR++ G + + DF+DVMLS + +
Sbjct: 247 KLGWLDLQGHEKEMKQTRRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGK 306
Query: 307 LS--GYDVDTVAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRER 366
LS YD +T K TCLALILGG T+ +TWA+SLLLNN++ LKKVQ E+D VGR+R
Sbjct: 307 LSHLQYDANTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDR 366
Query: 367 QVNESDIKNLIYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQ 426
V +SDIKNL+YLQA++KETLRLY AAPL E++EDCTVAGY++P GTRL VN+ K+Q
Sbjct: 367 NVEDSDIKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQ 426
Query: 427 RDPLVWENPGEFCPERFITSQ-KNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAK 486
RDP V+ P EF PERFIT + K+FDV+GQN +L+PFG+GRR CPG S A+Q++HL LA+
Sbjct: 427 RDPKVYMEPNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLAR 486
Query: 487 LLHGFEIKRPSEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIY 518
LH FE+K + P++M ES GL IT+ TPLEV+++ RL +++
Sbjct: 487 FLHSFEVKTVLDRPVDMSESPGLTITKATPLEVLINPRLKRELF 511
BLAST of MC11g_new0043 vs. NCBI nr
Match:
XP_022150894.1 (cytochrome P450 CYP82D47-like [Momordica charantia])
HSP 1 Score: 1045 bits (2702), Expect = 0.0
Identity = 518/518 (100.00%), Postives = 518/518 (100.00%), Query Frame = 0
Query: 1 MEFPHVSAAILALLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGKEPAHAT 60
MEFPHVSAAILALLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGKEPAHAT
Sbjct: 1 MEFPHVSAAILALLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGKEPAHAT 60
Query: 61 LGRMADAHGPIFMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLGYEYAMF 120
LGRMADAHGPIFMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLGYEYAMF
Sbjct: 61 LGRMADAHGPIFMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLGYEYAMF 120
Query: 121 GTSPYGSHWKHVPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGGGGKALV 180
GTSPYGSHWKHVPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGGGGKALV
Sbjct: 121 GTSPYGSHWKHVPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGGGGKALV 180
Query: 181 EMTTWFGDITLNTIFRIVVGKRFSTAFEGSGGEQYHKALSDFFDLFGAFIPSDSFPFLSW 240
EMTTWFGDITLNTIFRIVVGKRFSTAFEGSGGEQYHKALSDFFDLFGAFIPSDSFPFLSW
Sbjct: 181 EMTTWFGDITLNTIFRIVVGKRFSTAFEGSGGEQYHKALSDFFDLFGAFIPSDSFPFLSW 240
Query: 241 LDLGGYEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKDDELSGY 300
LDLGGYEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKDDELSGY
Sbjct: 241 LDLGGYEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKDDELSGY 300
Query: 301 DVDTVAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRERQVNESD 360
DVDTVAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRERQVNESD
Sbjct: 301 DVDTVAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRERQVNESD 360
Query: 361 IKNLIYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQRDPLVW 420
IKNLIYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQRDPLVW
Sbjct: 361 IKNLIYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQRDPLVW 420
Query: 421 ENPGEFCPERFITSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKLLHGFEI 480
ENPGEFCPERFITSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKLLHGFEI
Sbjct: 421 ENPGEFCPERFITSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKLLHGFEI 480
Query: 481 KRPSEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIYE 518
KRPSEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIYE
Sbjct: 481 KRPSEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIYE 518
BLAST of MC11g_new0043 vs. NCBI nr
Match:
XP_022137974.1 (cytochrome P450 CYP82D47-like [Momordica charantia])
HSP 1 Score: 847 bits (2189), Expect = 2.09e-306
Identity = 420/520 (80.77%), Postives = 460/520 (88.46%), Query Frame = 0
Query: 1 MEFPHVSAA-ILALLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGKEPAHA 60
M+F HVSAA I ALLF FYALFTI +RF +RRKNLPPEAGGGWPVIGHLH LSGKEPAH
Sbjct: 33 MDFLHVSAATIFALLFFFYALFTIPTRFVSRRKNLPPEAGGGWPVIGHLHQLSGKEPAHT 92
Query: 61 TLGRMADAHGPIFMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLGYEYAM 120
TLG+MADAHGPIFML+ G HRALVVSGWEI+RECFTTNDKKFASRPK+AAAKLLGYEYAM
Sbjct: 93 TLGKMADAHGPIFMLKLGTHRALVVSGWEIARECFTTNDKKFASRPKLAAAKLLGYEYAM 152
Query: 121 FGTSPYGSHWKHVPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGGGGKAL 180
FGTSPYGSHWKHV KI TLEVLANHRLEKLQHIR+SEVESSIKRL+ELCV K L
Sbjct: 153 FGTSPYGSHWKHVRKIATLEVLANHRLEKLQHIRRSEVESSIKRLHELCV-----NKKVL 212
Query: 181 VEMTTWFGDITLNTIFRIVVGKRFSTAFEGSGGEQYHKALSDFFDLFGAFIPSDSFPFLS 240
VEM WFG++TLNTIFR+VVGKRFSTAFEGS GEQY KAL DF LFGAF+PSDSFPFLS
Sbjct: 213 VEMKAWFGEVTLNTIFRMVVGKRFSTAFEGSSGEQYQKALRDFLYLFGAFVPSDSFPFLS 272
Query: 241 WLDLGGYEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEH-DFMDVMLSTVKDDELS 300
WLDLGGYEKAM+ TAKVLDEVL+KWL EH++R G+EM++ DFMDVMLSTVKD+E S
Sbjct: 273 WLDLGGYEKAMKKTAKVLDEVLEKWLTEHKQRRISSGMEMDQGLDFMDVMLSTVKDEEFS 332
Query: 301 GYDVDTVAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRERQVNE 360
YD DTV KATCLALILGGF TT+ E+TW LSLLLN+E ALKKVQ ELDE VGRERQV +
Sbjct: 333 DYDADTVTKATCLALILGGFDTTTTELTWGLSLLLNDEGALKKVQFELDEHVGRERQVED 392
Query: 361 SDIKNLIYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQRDPL 420
SD+KNLIYLQAVVKETLR+Y AAPLSVPHES+ED TVAGY++ GTRL VNLHKLQRDPL
Sbjct: 393 SDMKNLIYLQAVVKETLRMYPAAPLSVPHESLEDVTVAGYYVSAGTRLLVNLHKLQRDPL 452
Query: 421 VWENPGEFCPERFITSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKLLHGF 480
VWENP EFCPERF+TSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQ M+L LAKLLH F
Sbjct: 453 VWENPNEFCPERFLTSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQAMYLILAKLLHEF 512
Query: 481 EIKRPSEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIYE 518
+I RPSEEPLNMEES GLN T+KTPL+V+++ RLS ++YE
Sbjct: 513 DICRPSEEPLNMEESVGLNNTKKTPLQVIITPRLSVRVYE 547
BLAST of MC11g_new0043 vs. NCBI nr
Match:
XP_038897312.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])
HSP 1 Score: 766 bits (1978), Expect = 1.07e-274
Identity = 378/506 (74.70%), Postives = 430/506 (84.98%), Query Frame = 0
Query: 13 LLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGKEPAHATLGRMADAHGPIF 72
LL + YALF +S G R K LPP+ GG WPVIGHL LL+GKE H TLG++AD +GPIF
Sbjct: 23 LLIILYALF--NSFIGGRHKRLPPQPGGAWPVIGHLPLLTGKELIHNTLGKLADTYGPIF 82
Query: 73 MLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLGYEYAMFGTSPYGSHWKHV 132
MLR G ++ LVVSGWE+++ECFTTNDK FASRPK AAA+LLGY YAMFGTSPYGSHW+HV
Sbjct: 83 MLRLGTNKTLVVSGWEVAKECFTTNDKIFASRPKFAAAELLGYNYAMFGTSPYGSHWRHV 142
Query: 133 PKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGGGGKALVEMTTWFGDITLN 192
KIV LE+LANHRLEKLQHI +SEV++SI+RL+ELC N+ KALVEM TWFGDITLN
Sbjct: 143 RKIVMLELLANHRLEKLQHISRSEVQNSIERLHELCKSNK----KALVEMETWFGDITLN 202
Query: 193 TIFRIVVGKRFSTAFEGSGGEQYHKALSDFFDLFGAFIPSDSFPFLSWLDLGGYEKAMRN 252
TIFR+VVGKRFSTAFEGSGGE+Y AL FF+LF AF+PSD FPFL W D GGY+K M+
Sbjct: 203 TIFRMVVGKRFSTAFEGSGGEEYQNALKGFFNLFTAFVPSDLFPFLRWFDFGGYKKTMKK 262
Query: 253 TAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKDDELSGYDVDTVAKATCLA 312
TAKV+DE+ DKWL+EHRE+ +FDGV EE D M +MLS KD++ SGYDVDTV K+TCLA
Sbjct: 263 TAKVMDEMHDKWLREHREKRNFDGVGSEEQDLMHIMLSITKDEDFSGYDVDTVIKSTCLA 322
Query: 313 LILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRERQVNESDIKNLIYLQAVVK 372
+ILGGFHTT+VEM WALSLLLNNEEALKKVQ ELDE VGRERQV+ESDI +LIYLQAVVK
Sbjct: 323 MILGGFHTTTVEMVWALSLLLNNEEALKKVQSELDELVGRERQVSESDIHDLIYLQAVVK 382
Query: 373 ETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQRDPLVWENPGEFCPERFI 432
ETLRLY AA LSVPHESIEDCTVAGYHIP GTRL+VNL+KLQ DP VWE+P EF PERF+
Sbjct: 383 ETLRLYPAAQLSVPHESIEDCTVAGYHIPAGTRLWVNLYKLQSDPNVWESPREFRPERFL 442
Query: 433 TSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKLLHGFEIKRPSEEPLNMEE 492
+KNFDVKGQ+PQ IPFGAGRRICPG+SFA QVM L+LA+LLH FEIKRPSEEPLNMEE
Sbjct: 443 AIEKNFDVKGQSPQFIPFGAGRRICPGISFATQVMQLSLARLLHEFEIKRPSEEPLNMEE 502
Query: 493 SAGLNITRKTPLEVVLSQRLSAQIYE 518
S LNIT+K PLEVVL+ RLS QIY+
Sbjct: 503 SVALNITKKIPLEVVLTSRLSPQIYK 522
BLAST of MC11g_new0043 vs. NCBI nr
Match:
XP_038898814.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])
HSP 1 Score: 755 bits (1950), Expect = 1.80e-270
Identity = 369/522 (70.69%), Postives = 435/522 (83.33%), Query Frame = 0
Query: 4 PHVS-------AAILALLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGKEP 63
PH+S A IL LLF YAL + F R LPP+ GG WPVIGHL LL+GKE
Sbjct: 6 PHISSSNLAQPAIILTLLFFLYALLIL---FRGRHTRLPPQPGGAWPVIGHLPLLTGKEL 65
Query: 64 AHATLGRMADAHGPIFMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLGYE 123
H TLG++ADA+GPIFMLR G ++ L+VSGWE+++ECFTTNDKKFASRPK AAA+LLGY
Sbjct: 66 IHNTLGKLADAYGPIFMLRLGSNKTLIVSGWEVAKECFTTNDKKFASRPKFAAAELLGYN 125
Query: 124 YAMFGTSPYGSHWKHVPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGGGG 183
YAMFGTSPYGSHW+HV KIV LE+LANHRLEKLQHI +SEV+SSI+ L+ELC N+
Sbjct: 126 YAMFGTSPYGSHWRHVRKIVMLELLANHRLEKLQHIPRSEVQSSIETLHELCRSNK---- 185
Query: 184 KALVEMTTWFGDITLNTIFRIVVGKRFSTAFEGSGGEQYHKALSDFFDLFGAFIPSDSFP 243
KALVEM TWFGDITLN+IFR+VVGKRFSTAFE SGGE+YH A+ DFF+LF +PSD FP
Sbjct: 186 KALVEMETWFGDITLNSIFRMVVGKRFSTAFEESGGEKYHNAMKDFFNLFTVLVPSDLFP 245
Query: 244 FLSWLDLGGYEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKDDE 303
FL W D GGY+K M+ TAKV+DE+L KWL+EHRE+ + DGV MEE DFM VMLS ++++
Sbjct: 246 FLRWFDFGGYKKTMKETAKVMDEMLHKWLQEHREKRNSDGVGMEEQDFMHVMLSITEEED 305
Query: 304 LSGYDVDTVAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRERQV 363
SGYD+D V K+TCLA+ILGG+HTT+ EM WALSLL+NNEE+LKKVQ ELDEQVGRER+V
Sbjct: 306 FSGYDIDNVIKSTCLAVILGGYHTTTTEMVWALSLLVNNEESLKKVQFELDEQVGREREV 365
Query: 364 NESDIKNLIYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQRD 423
ESD+ NLIYLQAVVKETLRLY AA LSVPHESIEDCTVAGYHI GTRL+VNL+KLQRD
Sbjct: 366 RESDMNNLIYLQAVVKETLRLYPAAQLSVPHESIEDCTVAGYHIRAGTRLWVNLYKLQRD 425
Query: 424 PLVWENPGEFCPERFITSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKLLH 483
P VWE+P EF PERF+TS+KNFDVKGQ+PQ IPFG GRRICPG+SFA++VMHL+LA+LLH
Sbjct: 426 PCVWESPREFRPERFLTSEKNFDVKGQSPQFIPFGVGRRICPGISFAMKVMHLSLARLLH 485
Query: 484 GFEIKRPSEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIYE 518
FEI RPSEEP++MEE GLNI++K PLEVV++ R+S QIY+
Sbjct: 486 EFEIGRPSEEPVSMEEGVGLNISKKIPLEVVITPRISPQIYK 520
BLAST of MC11g_new0043 vs. NCBI nr
Match:
XP_022982222.1 (cytochrome P450 CYP82D47-like [Cucurbita maxima])
HSP 1 Score: 752 bits (1941), Expect = 4.22e-269
Identity = 367/524 (70.04%), Postives = 429/524 (81.87%), Query Frame = 0
Query: 1 MEFPHVSAA------ILALLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGK 60
ME PH+S+ I ALLF YALFTI F R LPP+ G WPVIGHL LL+ K
Sbjct: 1 MEIPHISSPTTALPLIFALLFFLYALFTI---FSANRNRLPPQPRGAWPVIGHLPLLTAK 60
Query: 61 EPAHATLGRMADAHGPIFMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLG 120
EP H TLG++AD +GPIFMLR G HR L+VSGWE++RECFT NDK FASRPK AAA+LLG
Sbjct: 61 EPNHTTLGKLADVYGPIFMLRLGAHRTLIVSGWEVARECFTVNDKAFASRPKFAAAQLLG 120
Query: 121 YEYAMFGTSPYGSHWKHVPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGG 180
Y+YAMFG SPYGSHW H+ KI TLE+LANHRLEKL+HI +SEV+SSI++L++L +++GG
Sbjct: 121 YDYAMFGLSPYGSHWSHLRKIATLELLANHRLEKLRHIPRSEVQSSIQKLHQLGKLSDGG 180
Query: 181 GGKALVEMTTWFGDITLNTIFRIVVGKRFSTAFEGSGGEQYHKALSDFFDLFGAFIPSDS 240
KALVEM TWFG+I LNTIFR+VVGKRF+TAFEGSGG Y AL DFF LF AF+PSD
Sbjct: 181 K-KALVEMETWFGEIALNTIFRMVVGKRFTTAFEGSGGALYRHALEDFFSLFTAFVPSDL 240
Query: 241 FPFLSWLDLGGYEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKD 300
FPFL W D GG++KAM+ TAKVLDE+LDKWL EHRER + VE EE DFM VMLS + +
Sbjct: 241 FPFLKWFDFGGHKKAMKKTAKVLDEMLDKWLTEHRERRNSGEVEPEEQDFMHVMLSLIDN 300
Query: 301 DELSGYDVDTVAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRER 360
+E SGYD DT+ K+TCLALILGGFHTT+ EMTWALSLLLN EEA +KVQ ELDE VGR+R
Sbjct: 301 EEFSGYDADTIIKSTCLALILGGFHTTTTEMTWALSLLLNREEAYEKVQKELDEHVGRDR 360
Query: 361 QVNESDIKNLIYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQ 420
V ESD+KNLIYLQAVVKETLR+Y AAPLSVPHES EDC VAGYHIP TRL+VNL++LQ
Sbjct: 361 VVRESDLKNLIYLQAVVKETLRMYPAAPLSVPHESTEDCIVAGYHIPAKTRLWVNLYRLQ 420
Query: 421 RDPLVWENPGEFCPERFITSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKL 480
RDP VWENP EFCPERF+T+QK FDVKG+NPQL PFG GRRICP ++FA+QV+HL+LA+L
Sbjct: 421 RDPSVWENPNEFCPERFLTTQKEFDVKGRNPQLTPFGVGRRICPALTFALQVLHLSLARL 480
Query: 481 LHGFEIKRPSEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIYE 518
LH FEI RPSEEPL+MEE A LNIT+K PL+V+L+ RLS ++ E
Sbjct: 481 LHEFEIGRPSEEPLDMEEIAALNITKKVPLKVILTPRLSTEVCE 520
BLAST of MC11g_new0043 vs. ExPASy TrEMBL
Match:
A0A6J1DBE5 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111018907 PE=3 SV=1)
HSP 1 Score: 1045 bits (2702), Expect = 0.0
Identity = 518/518 (100.00%), Postives = 518/518 (100.00%), Query Frame = 0
Query: 1 MEFPHVSAAILALLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGKEPAHAT 60
MEFPHVSAAILALLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGKEPAHAT
Sbjct: 1 MEFPHVSAAILALLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGKEPAHAT 60
Query: 61 LGRMADAHGPIFMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLGYEYAMF 120
LGRMADAHGPIFMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLGYEYAMF
Sbjct: 61 LGRMADAHGPIFMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLGYEYAMF 120
Query: 121 GTSPYGSHWKHVPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGGGGKALV 180
GTSPYGSHWKHVPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGGGGKALV
Sbjct: 121 GTSPYGSHWKHVPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGGGGKALV 180
Query: 181 EMTTWFGDITLNTIFRIVVGKRFSTAFEGSGGEQYHKALSDFFDLFGAFIPSDSFPFLSW 240
EMTTWFGDITLNTIFRIVVGKRFSTAFEGSGGEQYHKALSDFFDLFGAFIPSDSFPFLSW
Sbjct: 181 EMTTWFGDITLNTIFRIVVGKRFSTAFEGSGGEQYHKALSDFFDLFGAFIPSDSFPFLSW 240
Query: 241 LDLGGYEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKDDELSGY 300
LDLGGYEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKDDELSGY
Sbjct: 241 LDLGGYEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKDDELSGY 300
Query: 301 DVDTVAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRERQVNESD 360
DVDTVAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRERQVNESD
Sbjct: 301 DVDTVAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRERQVNESD 360
Query: 361 IKNLIYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQRDPLVW 420
IKNLIYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQRDPLVW
Sbjct: 361 IKNLIYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQRDPLVW 420
Query: 421 ENPGEFCPERFITSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKLLHGFEI 480
ENPGEFCPERFITSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKLLHGFEI
Sbjct: 421 ENPGEFCPERFITSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKLLHGFEI 480
Query: 481 KRPSEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIYE 518
KRPSEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIYE
Sbjct: 481 KRPSEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIYE 518
BLAST of MC11g_new0043 vs. ExPASy TrEMBL
Match:
A0A6J1C8S8 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009252 PE=3 SV=1)
HSP 1 Score: 847 bits (2189), Expect = 1.01e-306
Identity = 420/520 (80.77%), Postives = 460/520 (88.46%), Query Frame = 0
Query: 1 MEFPHVSAA-ILALLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGKEPAHA 60
M+F HVSAA I ALLF FYALFTI +RF +RRKNLPPEAGGGWPVIGHLH LSGKEPAH
Sbjct: 33 MDFLHVSAATIFALLFFFYALFTIPTRFVSRRKNLPPEAGGGWPVIGHLHQLSGKEPAHT 92
Query: 61 TLGRMADAHGPIFMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLGYEYAM 120
TLG+MADAHGPIFML+ G HRALVVSGWEI+RECFTTNDKKFASRPK+AAAKLLGYEYAM
Sbjct: 93 TLGKMADAHGPIFMLKLGTHRALVVSGWEIARECFTTNDKKFASRPKLAAAKLLGYEYAM 152
Query: 121 FGTSPYGSHWKHVPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGGGGKAL 180
FGTSPYGSHWKHV KI TLEVLANHRLEKLQHIR+SEVESSIKRL+ELCV K L
Sbjct: 153 FGTSPYGSHWKHVRKIATLEVLANHRLEKLQHIRRSEVESSIKRLHELCV-----NKKVL 212
Query: 181 VEMTTWFGDITLNTIFRIVVGKRFSTAFEGSGGEQYHKALSDFFDLFGAFIPSDSFPFLS 240
VEM WFG++TLNTIFR+VVGKRFSTAFEGS GEQY KAL DF LFGAF+PSDSFPFLS
Sbjct: 213 VEMKAWFGEVTLNTIFRMVVGKRFSTAFEGSSGEQYQKALRDFLYLFGAFVPSDSFPFLS 272
Query: 241 WLDLGGYEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEH-DFMDVMLSTVKDDELS 300
WLDLGGYEKAM+ TAKVLDEVL+KWL EH++R G+EM++ DFMDVMLSTVKD+E S
Sbjct: 273 WLDLGGYEKAMKKTAKVLDEVLEKWLTEHKQRRISSGMEMDQGLDFMDVMLSTVKDEEFS 332
Query: 301 GYDVDTVAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRERQVNE 360
YD DTV KATCLALILGGF TT+ E+TW LSLLLN+E ALKKVQ ELDE VGRERQV +
Sbjct: 333 DYDADTVTKATCLALILGGFDTTTTELTWGLSLLLNDEGALKKVQFELDEHVGRERQVED 392
Query: 361 SDIKNLIYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQRDPL 420
SD+KNLIYLQAVVKETLR+Y AAPLSVPHES+ED TVAGY++ GTRL VNLHKLQRDPL
Sbjct: 393 SDMKNLIYLQAVVKETLRMYPAAPLSVPHESLEDVTVAGYYVSAGTRLLVNLHKLQRDPL 452
Query: 421 VWENPGEFCPERFITSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKLLHGF 480
VWENP EFCPERF+TSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQ M+L LAKLLH F
Sbjct: 453 VWENPNEFCPERFLTSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQAMYLILAKLLHEF 512
Query: 481 EIKRPSEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIYE 518
+I RPSEEPLNMEES GLN T+KTPL+V+++ RLS ++YE
Sbjct: 513 DICRPSEEPLNMEESVGLNNTKKTPLQVIITPRLSVRVYE 547
BLAST of MC11g_new0043 vs. ExPASy TrEMBL
Match:
A0A6J1IW32 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111481115 PE=3 SV=1)
HSP 1 Score: 752 bits (1941), Expect = 2.04e-269
Identity = 367/524 (70.04%), Postives = 429/524 (81.87%), Query Frame = 0
Query: 1 MEFPHVSAA------ILALLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGK 60
ME PH+S+ I ALLF YALFTI F R LPP+ G WPVIGHL LL+ K
Sbjct: 1 MEIPHISSPTTALPLIFALLFFLYALFTI---FSANRNRLPPQPRGAWPVIGHLPLLTAK 60
Query: 61 EPAHATLGRMADAHGPIFMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLG 120
EP H TLG++AD +GPIFMLR G HR L+VSGWE++RECFT NDK FASRPK AAA+LLG
Sbjct: 61 EPNHTTLGKLADVYGPIFMLRLGAHRTLIVSGWEVARECFTVNDKAFASRPKFAAAQLLG 120
Query: 121 YEYAMFGTSPYGSHWKHVPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGG 180
Y+YAMFG SPYGSHW H+ KI TLE+LANHRLEKL+HI +SEV+SSI++L++L +++GG
Sbjct: 121 YDYAMFGLSPYGSHWSHLRKIATLELLANHRLEKLRHIPRSEVQSSIQKLHQLGKLSDGG 180
Query: 181 GGKALVEMTTWFGDITLNTIFRIVVGKRFSTAFEGSGGEQYHKALSDFFDLFGAFIPSDS 240
KALVEM TWFG+I LNTIFR+VVGKRF+TAFEGSGG Y AL DFF LF AF+PSD
Sbjct: 181 K-KALVEMETWFGEIALNTIFRMVVGKRFTTAFEGSGGALYRHALEDFFSLFTAFVPSDL 240
Query: 241 FPFLSWLDLGGYEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKD 300
FPFL W D GG++KAM+ TAKVLDE+LDKWL EHRER + VE EE DFM VMLS + +
Sbjct: 241 FPFLKWFDFGGHKKAMKKTAKVLDEMLDKWLTEHRERRNSGEVEPEEQDFMHVMLSLIDN 300
Query: 301 DELSGYDVDTVAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRER 360
+E SGYD DT+ K+TCLALILGGFHTT+ EMTWALSLLLN EEA +KVQ ELDE VGR+R
Sbjct: 301 EEFSGYDADTIIKSTCLALILGGFHTTTTEMTWALSLLLNREEAYEKVQKELDEHVGRDR 360
Query: 361 QVNESDIKNLIYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQ 420
V ESD+KNLIYLQAVVKETLR+Y AAPLSVPHES EDC VAGYHIP TRL+VNL++LQ
Sbjct: 361 VVRESDLKNLIYLQAVVKETLRMYPAAPLSVPHESTEDCIVAGYHIPAKTRLWVNLYRLQ 420
Query: 421 RDPLVWENPGEFCPERFITSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKL 480
RDP VWENP EFCPERF+T+QK FDVKG+NPQL PFG GRRICP ++FA+QV+HL+LA+L
Sbjct: 421 RDPSVWENPNEFCPERFLTTQKEFDVKGRNPQLTPFGVGRRICPALTFALQVLHLSLARL 480
Query: 481 LHGFEIKRPSEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIYE 518
LH FEI RPSEEPL+MEE A LNIT+K PL+V+L+ RLS ++ E
Sbjct: 481 LHEFEIGRPSEEPLDMEEIAALNITKKVPLKVILTPRLSTEVCE 520
BLAST of MC11g_new0043 vs. ExPASy TrEMBL
Match:
A0A6J1FQP2 (cytochrome P450 CYP82D47-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446066 PE=3 SV=1)
HSP 1 Score: 751 bits (1940), Expect = 3.49e-269
Identity = 367/524 (70.04%), Postives = 430/524 (82.06%), Query Frame = 0
Query: 1 MEFPHVSAA------ILALLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGK 60
ME PH+S+ I ALL YALFTI F RK LPP+ G WPVIGHL LL+ K
Sbjct: 1 MEIPHISSPATALPLIFALLLFLYALFTI---FSAHRKRLPPQPRGAWPVIGHLPLLTAK 60
Query: 61 EPAHATLGRMADAHGPIFMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLG 120
EP H TLG++AD +GPIFMLR G +R L+VSGWE++RECFT NDK FASRPK AAA+LLG
Sbjct: 61 EPNHTTLGKLADVYGPIFMLRLGANRTLIVSGWEVARECFTVNDKAFASRPKFAAAQLLG 120
Query: 121 YEYAMFGTSPYGSHWKHVPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGG 180
Y+YAMFG SPYGSHW H+ KIVTLE+LANHRLEKL+HI +SEV+SSI++L++LC ++GG
Sbjct: 121 YDYAMFGLSPYGSHWSHLRKIVTLELLANHRLEKLRHIPRSEVQSSIQKLHQLCKSSDGG 180
Query: 181 GGKALVEMTTWFGDITLNTIFRIVVGKRFSTAFEGSGGEQYHKALSDFFDLFGAFIPSDS 240
KALVEM TWFG+I LNTIFR+VVGKRF+TAFEGSGG +Y AL DFF+LF F+PSD
Sbjct: 181 K-KALVEMETWFGEIALNTIFRMVVGKRFTTAFEGSGGAEYRHALEDFFNLFTVFVPSDL 240
Query: 241 FPFLSWLDLGGYEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKD 300
FPFL W D GG++KAM+ TAKV+DE+LDKWL EHRER + V EE DFM VMLS +
Sbjct: 241 FPFLKWFDFGGHKKAMKKTAKVMDEMLDKWLTEHRERRNSGEVAPEEQDFMHVMLSFIDT 300
Query: 301 DELSGYDVDTVAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRER 360
+E SGYD DT+ K+TCLALILGGFHTT+ EMTWALSLLLN EEA +KVQ ELDE VGR+R
Sbjct: 301 EEFSGYDADTIIKSTCLALILGGFHTTTTEMTWALSLLLNREEAYEKVQEELDEHVGRDR 360
Query: 361 QVNESDIKNLIYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQ 420
V ESD+K LIYLQAVVKETLR+Y AA LSVPHES EDCT+AGYHIP TRL+VNL+KLQ
Sbjct: 361 VVKESDLKILIYLQAVVKETLRMYPAAQLSVPHESTEDCTIAGYHIPAKTRLWVNLYKLQ 420
Query: 421 RDPLVWENPGEFCPERFITSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKL 480
RDP VWENP +FCPERF+T+QKNFDVKGQNPQLIPFGAGRRICP M FA+QV+HL+ A+L
Sbjct: 421 RDPSVWENPDQFCPERFLTTQKNFDVKGQNPQLIPFGAGRRICPAMIFALQVLHLSFARL 480
Query: 481 LHGFEIKRPSEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIYE 518
LH FEI+RPSEEPL+MEE+A LNIT+K PL V+L+ RLS ++ E
Sbjct: 481 LHEFEIRRPSEEPLDMEETAALNITKKVPLRVILTPRLSYEVCE 520
BLAST of MC11g_new0043 vs. ExPASy TrEMBL
Match:
A0A6J1FIL1 (cytochrome P450 CYP82D47-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446066 PE=3 SV=1)
HSP 1 Score: 748 bits (1931), Expect = 8.15e-268
Identity = 366/524 (69.85%), Postives = 426/524 (81.30%), Query Frame = 0
Query: 1 MEFPHVSA------AILALLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGK 60
ME PH+S+ AI ALLF YALFTI F RK LPP+ G WPVIGHL LLSGK
Sbjct: 1 MELPHISSTTIALPAIFALLFFLYALFTI---FSAHRKRLPPQPRGAWPVIGHLPLLSGK 60
Query: 61 EPAHATLGRMADAHGPIFMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLG 120
EP H LG++AD +GPIFMLR G HR L+VSGWE++REC T NDK FASRPK AAA+LLG
Sbjct: 61 EPHHTALGKLADVYGPIFMLRLGAHRTLIVSGWEVARECLTVNDKAFASRPKFAAAQLLG 120
Query: 121 YEYAMFGTSPYGSHWKHVPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGG 180
Y+YAMF SPYGSHW H+ KI TLE+LANHRLEKLQHI +SEV+ SI++L++ C N GG
Sbjct: 121 YDYAMFALSPYGSHWSHLRKIATLELLANHRLEKLQHIPRSEVQISIEKLHQFCKSNNGG 180
Query: 181 GGKALVEMTTWFGDITLNTIFRIVVGKRFSTAFEGSGGEQYHKALSDFFDLFGAFIPSDS 240
KAL+EM TWFG++ LNTIFR+VVGKRF+TAFEGSGG +Y AL DFF+LF AF+PSD
Sbjct: 181 N-KALMEMETWFGEMALNTIFRMVVGKRFTTAFEGSGGAEYRHALEDFFNLFTAFVPSDL 240
Query: 241 FPFLSWLDLGGYEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKD 300
F FL W D GG++KAM+ TAKVLD++LDKWL EHRER + V EE DFM VMLS + +
Sbjct: 241 FAFLKWFDFGGHKKAMKKTAKVLDKMLDKWLAEHRERRNSGEVTPEEQDFMHVMLSFIDN 300
Query: 301 DELSGYDVDTVAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRER 360
+E GYD DTV K+TCLALILGGFHTT+ EMTWALSLLLN EEA +KVQ ELDE VGR+R
Sbjct: 301 EEFPGYDADTVIKSTCLALILGGFHTTTTEMTWALSLLLNREEAYEKVQEELDEHVGRDR 360
Query: 361 QVNESDIKNLIYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQ 420
V ESD+K LIYLQAVVKETLR+Y AA LSVPHES EDCT+AGYHIP TRL+VNL+KLQ
Sbjct: 361 VVKESDLKILIYLQAVVKETLRMYPAAQLSVPHESTEDCTIAGYHIPAKTRLWVNLYKLQ 420
Query: 421 RDPLVWENPGEFCPERFITSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKL 480
RDP VWENP +FCPERF+T+QKNFDVKGQNPQLIPFGAGRRICP M FA+QV+HL+ A+L
Sbjct: 421 RDPSVWENPDQFCPERFLTTQKNFDVKGQNPQLIPFGAGRRICPAMIFALQVLHLSFARL 480
Query: 481 LHGFEIKRPSEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIYE 518
LH FEI+RPSEEPL+MEE+A LNIT+K PL V+L+ RLS ++ E
Sbjct: 481 LHEFEIRRPSEEPLDMEETAALNITKKVPLRVILTPRLSYEVCE 520
BLAST of MC11g_new0043 vs. TAIR 10
Match:
AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )
HSP 1 Score: 508.8 bits (1309), Expect = 5.0e-144
Identity = 257/518 (49.61%), Postives = 355/518 (68.53%), Query Frame = 0
Query: 13 LLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGKEP-AHATLGRMADAHGPI 72
L+F+F ALF S + + P G WP+IGHLHLL GKE + TLG+MAD +GP
Sbjct: 13 LVFVFIALFKKSKK---PKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPA 72
Query: 73 FMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLGYEYAMFGTSPYGSHWKH 132
L+ G + A VVS +E++++CFT NDK ASRP AAAK +GY +A+FG +PY + W+
Sbjct: 73 MSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWRE 132
Query: 133 VPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGGGGKALVEMTTWFGDITL 192
+ KI T+E+L+N RL+ L+H+R SE+ +K L L N GG +V++ +W D+TL
Sbjct: 133 MRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKN-GGTKPVMVDLKSWLEDMTL 192
Query: 193 NTIFRIVVGKRFSTAFEGSG---------GEQYHKALSDFFDLFGAFIPSDSFPFLSWLD 252
N I R+V GKR+ F G G Q KA++ FF L G F SD+FP LS+ D
Sbjct: 193 NMIVRMVAGKRY---FGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFD 252
Query: 253 LGGYEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKDDELS--GY 312
L G+EK M+ T LD +L++W++ HR++ F G + + DF+DVM+S + +LS Y
Sbjct: 253 LQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSLAEQGKLSHLQY 312
Query: 313 DVDTVAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRERQVNESD 372
D +T K+TCLALILGG T++ +TWA+SLLLNN+E LKK Q E+D VGR+R V +SD
Sbjct: 313 DANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSD 372
Query: 373 IKNLIYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQRDPLVW 432
I+NL+YLQA++KETLRLY A PL P E++EDCTVAGY++P GTRL VN+ K+QRDP V+
Sbjct: 373 IENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVY 432
Query: 433 ENPGEFCPERFITSQ-KNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKLLHGFE 492
P EF PERFIT + K FDV+GQN +L+PFG+GRR CPG S A+QV+HL LA+ LH F+
Sbjct: 433 MEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHSFD 492
Query: 493 IKRPSEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIY 518
+K + P++M E+ GL I + TPLEV++S R+ +++
Sbjct: 493 VKTVMDMPVDMSENPGLTIPKATPLEVLISPRIKEELF 523
BLAST of MC11g_new0043 vs. TAIR 10
Match:
AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )
HSP 1 Score: 486.5 bits (1251), Expect = 2.7e-137
Identity = 246/514 (47.86%), Postives = 345/514 (67.12%), Query Frame = 0
Query: 13 LLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGKEP-AHATLGRMADAHGPI 72
L+F+F ALF S + + P G WP+IGHLHLLSGKE + TLG+MAD +GP
Sbjct: 13 LVFVFIALFKKSKK---PKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQYGPA 72
Query: 73 FMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLGYEYAMFGTSPYGSHWKH 132
LR G VVS +E++++CFT NDK ASRP AAAK +GY+ A+FG +PY + W+
Sbjct: 73 MSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWRE 132
Query: 133 VPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGGGGKALVEMTTWFGDITL 192
+ KI TLE+L+N RL+ L+H+R SE+ ++ L L V +GG +V++ +W D++L
Sbjct: 133 MRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWV-KKGGSEPVMVDLKSWLEDMSL 192
Query: 193 NTIFRIVVGKRF-----STAFEGSGGEQYHKALSDFFDLFGAFIPSDSFPFLSWLDLGGY 252
N + R+V GKR+ + + Q K +++FF L G F SD+FP L W D G+
Sbjct: 193 NMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFDFQGH 252
Query: 253 EKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKDDELS--GYDVDT 312
EK M+ T + LD +L++W++ HR++ G + + DF+DVMLS + + S +D T
Sbjct: 253 EKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKFSHLQHDAIT 312
Query: 313 VAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRERQVNESDIKNL 372
K+TCLALILGG T+ +TWA+SLLLNN++ LKK Q E+D VGR+R V +SDI+NL
Sbjct: 313 SIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENL 372
Query: 373 IYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQRDPLVWENPG 432
+Y+QA++KETLRLY A PL E+IEDCTVAGY++ GTR+ VN+ K+QRDP V+ P
Sbjct: 373 VYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPN 432
Query: 433 EFCPERFITSQ-KNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKLLHGFEIKRP 492
EF PERFIT + K FDV+GQN +L+PFG+GRR CPG S A+QV+HL LA+ L F++K
Sbjct: 433 EFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKTV 492
Query: 493 SEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIY 518
+ P++M ES GL I + TPLE+++S RL +Y
Sbjct: 493 MDMPVDMTESPGLTIPKATPLEILISPRLKEGLY 522
BLAST of MC11g_new0043 vs. TAIR 10
Match:
AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )
HSP 1 Score: 472.6 bits (1215), Expect = 4.0e-133
Identity = 250/524 (47.71%), Postives = 342/524 (65.27%), Query Frame = 0
Query: 7 SAAILALLFLFYALFTISSRFGTRRKNLPPEAGGGWPVIGHLHLLSGKEP-AHATLGRMA 66
S + L+F+F ALF S + + P G WP+IGHLHLL GKE + TLG+MA
Sbjct: 7 SLFVSILVFVFIALFKKSKK---PKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMA 66
Query: 67 DAHGPIFMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLGYEYAMFGTSPY 126
D +GP LR G V S +E++++CFT NDK AS AAAK +GY +
Sbjct: 67 DHYGPAMSLRLGSSETFVGSSFEVAKDCFTVNDKALASL-MTAAAKHMGYVF-------- 126
Query: 127 GSHWKHVPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGGGGKALVEMTTW 186
W + KI +E+L+N RL+ L ++R SE+ +K L L V +GG +V++ +W
Sbjct: 127 ---WLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWV-KKGGSEPVMVDLKSW 186
Query: 187 FGDITLNTIFRIVVGKRFSTAFEGSGGE---------QYHKALSDFFDLFGAFIPSDSFP 246
D+ N I R+V GKR+ F G G E Q+ K ++ FF L G F SD+FP
Sbjct: 187 LEDMIANMIMRMVAGKRY---FGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFP 246
Query: 247 FLSWLDLGGYEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKDDE 306
L WLDL G+EK M+ T + LD +L++W++ HR++ G + + DF+DVMLS + +
Sbjct: 247 KLGWLDLQGHEKEMKQTRRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGK 306
Query: 307 LS--GYDVDTVAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRER 366
LS YD +T K TCLALILGG T+ +TWA+SLLLNN++ LKKVQ E+D VGR+R
Sbjct: 307 LSHLQYDANTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDR 366
Query: 367 QVNESDIKNLIYLQAVVKETLRLYLAAPLSVPHESIEDCTVAGYHIPIGTRLFVNLHKLQ 426
V +SDIKNL+YLQA++KETLRLY AAPL E++EDCTVAGY++P GTRL VN+ K+Q
Sbjct: 367 NVEDSDIKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQ 426
Query: 427 RDPLVWENPGEFCPERFITSQ-KNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAK 486
RDP V+ P EF PERFIT + K+FDV+GQN +L+PFG+GRR CPG S A+Q++HL LA+
Sbjct: 427 RDPKVYMEPNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLAR 486
Query: 487 LLHGFEIKRPSEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIY 518
LH FE+K + P++M ES GL IT+ TPLEV+++ RL +++
Sbjct: 487 FLHSFEVKTVLDRPVDMSESPGLTITKATPLEVLINPRLKRELF 511
BLAST of MC11g_new0043 vs. TAIR 10
Match:
AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )
HSP 1 Score: 379.4 bits (973), Expect = 4.6e-105
Identity = 211/486 (43.42%), Postives = 290/486 (59.67%), Query Frame = 0
Query: 36 PEAGGGWPVIGHLHLLSGKEPAHATLGRMADAHGPIFMLRFGMHRALVVSGWEISRECFT 95
PE G P+ GHLHLL GK+ L M+ HGPIF L+ G +R +V S + ++CFT
Sbjct: 40 PEPLGALPLFGHLHLLRGKKLLCKKLAAMSQKHGPIFSLKLGFYRLVVASDPKTVKDCFT 99
Query: 96 TNDKKFASRPKVAAAKLLGYEYAMFGTSPYGSHWKHVPKIVTLEVLANHRLEKLQHIRKS 155
TND A+RP +A + +GY A +PYG +W+ + KIVT+ + +NH +E L HIR S
Sbjct: 100 TNDLATATRPNIAFGRYVGYNNASLTLAPYGDYWRELRKIVTVHLFSNHSIEMLGHIRSS 159
Query: 156 EVESSIKRLNELCVINEGGGGKALVEMTTWFGDITLNTIFRIVVGKRFSTAFEGSGGEQY 215
EV + IK L +G GG ++V++ F +T N I R +VGKR S +Y
Sbjct: 160 EVNTLIKHL------YKGNGGTSIVKIDMLFEFLTFNIILRKMVGKRIGFGEVNSDEWRY 219
Query: 216 HKALSDFFDLFGAFIPSDSFPFLSWLDLGGYEKAMRNTAKVLDEVLDKWLKEH---RERN 275
+AL L + D P+L WLD M+ K LD V KWL EH R RN
Sbjct: 220 KEALKHCEYLAVIPMIGDVIPWLGWLDF-AKNSQMKRLFKELDSVNTKWLHEHLKKRSRN 279
Query: 276 SFDGVEMEEHDFMDVMLSTVKDD-ELSGYDVDTVAKATCLALILGGFHTTSVEMTWALSL 335
D +E MD++L + +D +SG+ D + KAT LAL L G +TS+ +TWA+SL
Sbjct: 280 EKD----QERTIMDLLLDILPEDIVISGHVRDVIVKATILALTLTGSDSTSITLTWAVSL 339
Query: 336 LLNNEEALKKVQLELDEQVGRERQVNESDIKNLIYLQAVVKETLRLYLAAPLSVPHESIE 395
LLNN AL+ Q E+D VG+ R + ESDI+NL YLQA+VKET RLY APL+ E+ E
Sbjct: 340 LLNNPAALEAAQEEIDNSVGKGRWIEESDIQNLKYLQAIVKETHRLYPPAPLTGIREARE 399
Query: 396 DCTVAGYHIPIGTRLFVNLHKLQRDPLVWENPGEFCPERFITSQKNFDVKGQNPQLIPFG 455
DC V GY + GTRL VN+ KL RDP +W +P F PERF+ + + N + IPFG
Sbjct: 400 DCFVGGYRVEKGTRLLVNIWKLHRDPKIWPDPKTFKPERFMEDKS--QCEKSNFEYIPFG 459
Query: 456 AGRRICPGMSFAIQVMHLTLAKLLHGFEIKRPSEEPLNMEESAGLNITRKTPLEVVLSQR 515
+GRR CPG++ ++V+H LA+LL GFE+ + S+EPL+M E GL + + P+EVV+ R
Sbjct: 460 SGRRSCPGVNLGLRVVHFVLARLLQGFELHKVSDEPLDMAEGPGLALPKINPVEVVVMPR 512
Query: 516 LSAQIY 518
L ++Y
Sbjct: 520 LDPKLY 512
BLAST of MC11g_new0043 vs. TAIR 10
Match:
AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )
HSP 1 Score: 369.0 bits (946), Expect = 6.2e-102
Identity = 199/516 (38.57%), Postives = 297/516 (57.56%), Query Frame = 0
Query: 10 ILALLFLFYALFTISSRFGTR-RKNLPPEAGGGWPVIGHLHLLSGKEPAHATLGRMADAH 69
+L+ LF+F L I SR + +K+ P G WP++GHLHL P H T G MAD +
Sbjct: 8 LLSALFIFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGAMADVY 67
Query: 70 GPIFMLRFGMHRALVVSGWEISRECFTTNDKKFASRPKVAAAKLLGYEYAMFGTSPYGSH 129
GP+FM + G + ++++ E+++E +T +D K RP++ A+KLLGY + SPYG +
Sbjct: 68 GPVFMAKLGSIKVMIINSKEVAKEIYTVHD-KLLERPELTASKLLGYNDSFLTFSPYGLY 127
Query: 130 WKHVPKIVTLEVLANHRLEKLQHIRKSEVESSIKRLNELCVINEGGGGKALVEMTTWFGD 189
W+ + KI E+ + ++ R E + + + L LV+M F D
Sbjct: 128 WREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKRGKPKEGVLVDMKQEFID 187
Query: 190 ITLNTIFRIVVGKRF---STAFEGSGGEQYHKALSDFFDLFGAFIPSDSFPFLSWLDLGG 249
+T N +V GKR+ + E + K + +F D F F+ SD P L +LD
Sbjct: 188 LTANISLMMVSGKRYFGENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVLGFLD-WK 247
Query: 250 YEKAMRNTAKVLDEVLDKWLKEHRERNSFDGVEMEEHDFMDVMLSTVKDDELSGY-DVDT 309
++ M+ TAK LD+V + W++EH+ + S G E+D++D+++ + D++ G D T
Sbjct: 248 TKRGMKRTAKGLDKVAEGWIEEHKNKRSDHG--RSENDYLDILIKILGQDKIPGLSDTHT 307
Query: 310 VAKATCLALILGGFHTTSVEMTWALSLLLNNEEALKKVQLELDEQVGRERQVNESDIKNL 369
KA CL L+L G T V + WA+SLLLNN L+K Q ELD ++G+ER V E DIK+L
Sbjct: 308 KIKALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIGKERVVEELDIKDL 367
Query: 370 IYLQAVVKETLRLYLAAPLSVPHESIEDCTVA--GYHIPIGTRLFVNLHKLQRDPLVWEN 429
+YLQA+VKET RLY PL +ED +A H+P GT+L V+ K+ RDP VW N
Sbjct: 368 VYLQAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIHRDPNVWSN 427
Query: 430 PGEFCPERFITSQKNFDVKGQNPQLIPFGAGRRICPGMSFAIQVMHLTLAKLLHGFEIKR 489
P +F PERF+TS + DV GQ+ + PFG GRR CP + ++++H L + LH F++ R
Sbjct: 428 PEQFEPERFLTSNRELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHSFDLAR 487
Query: 490 PSEEPLNMEESAGLNITRKTPLEVVLSQRLSAQIYE 519
PS + ++M ES GL + TPLEV + RL +YE
Sbjct: 488 PSSQDVDMTESNGLVNHKATPLEVNIIPRLHKSLYE 519
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q9SZ46 | 7.1e-143 | 49.61 | Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=... | [more] |
| O49394 | 3.8e-136 | 47.86 | Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=... | [more] |
| A0A0N7F297 | 1.2e-134 | 47.67 | Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP... | [more] |
| O49858 | 3.5e-134 | 46.41 | Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1 | [more] |
| O49396 | 5.6e-132 | 47.71 | Cytochrome P450 82C3 OS=Arabidopsis thaliana OX=3702 GN=CYP82C3 PE=2 SV=3 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1DBE5 | 0.0 | 100.00 | cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111018907 PE=... | [more] |
| A0A6J1C8S8 | 1.01e-306 | 80.77 | cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009252 PE=... | [more] |
| A0A6J1IW32 | 2.04e-269 | 70.04 | cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111481115 PE=3 S... | [more] |
| A0A6J1FQP2 | 3.49e-269 | 70.04 | cytochrome P450 CYP82D47-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111... | [more] |
| A0A6J1FIL1 | 8.15e-268 | 69.85 | cytochrome P450 CYP82D47-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT4G31940.1 | 5.0e-144 | 49.61 | cytochrome P450, family 82, subfamily C, polypeptide 4 | [more] |
| AT4G31970.1 | 2.7e-137 | 47.86 | cytochrome P450, family 82, subfamily C, polypeptide 2 | [more] |
| AT4G31950.1 | 4.0e-133 | 47.71 | cytochrome P450, family 82, subfamily C, polypeptide 3 | [more] |
| AT3G25180.1 | 4.6e-105 | 43.42 | cytochrome P450, family 82, subfamily G, polypeptide 1 | [more] |
| AT2G25160.1 | 6.2e-102 | 38.57 | cytochrome P450, family 82, subfamily F, polypeptide 1 | [more] |
