MC11g_new0034 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g_new0034 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 1.2e-142
Identity = 263/526 (50.00%), Postives = 368/526 (69.96%), Query Frame = 0

Query: 10  IFSGVFALLLFLY-ALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAKEP-THITL 69
           +FS    +L+F++ ALF  S+K      + PAP    GAWP+IGHLH L  KE   + TL
Sbjct: 5   LFSLFVPILVFVFIALFKKSKKPK--YVKAPAP---SGAWPIIGHLHLLGGKEQLLYRTL 64

Query: 70  AKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLGYDYAMFG 129
            KMAD YGP  +L+ G  +A VVSS+E+A++ FT ND+  ASRP   A+K +GY++A+FG
Sbjct: 65  GKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFG 124

Query: 130 LSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVEN--EEKAVVEM 189
            +PY   WR+ R++AT+ELL+N RL+ L+HVR+ E+   +K+LY L  +N   +  +V++
Sbjct: 125 FAPYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVDL 184

Query: 190 KTWFGDVTLNTIFRMVVGKRF-----STAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQ 249
           K+W  D+TLN I RMV GKR+     S + +D   + +CKKA+  FF L GIF  SDAF 
Sbjct: 185 KSWLEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFP 244

Query: 250 FLSWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVV-K 309
            LS+FDL GHEK MK+T   LD   +++++ H+QQ+   F  ++  + DF+DVM+S+  +
Sbjct: 245 TLSFFDLQGHEKEMKQTGSELDVILERWIENHRQQR--KFSGTKENDSDFIDVMMSLAEQ 304

Query: 310 NEAESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGR 369
            +     YDA T IK+TCL +ILGG DT+  T+TWAISLLLNN+E LKKAQ E+D  VGR
Sbjct: 305 GKLSHLQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGR 364

Query: 370 ERQVNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKK 429
           +R V +SD++NL+YL+AI+KETLRLYP  PLL P E++EDCT  GYY+P GTRLIVNV K
Sbjct: 365 DRNVEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWK 424

Query: 430 LQRDPFVWEDPCEFRPERFLTSQ-NNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTL 489
           +QRDP V+ +P EFRPERF+T +   FDVRGQ+ + +PFG+GRR CPG S A+QV+HL L
Sbjct: 425 IQRDPKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGL 484

Query: 490 ANLLHGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVY 525
           A  LH F++    +  +DM E+ GLT  + TPLE++++PR+  +++
Sbjct: 485 ARFLHSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRIKEELF 523

BLAST of MC11g_new0034 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 6.5e-136
Identity = 251/515 (48.74%), Postives = 344/515 (66.80%), Query Frame = 0

Query: 15  FALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAKEPTHITLAKMADAY 74
           F +LL  +  F   + ++    ++  PP+  GAWP+IGHLH L+  +  H  L+  AD  
Sbjct: 14  FFVLLPCFFYFVWKKPNN----KIKEPPQPAGAWPIIGHLHLLARGDLPHKILSSFADKN 73

Query: 75  GPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLGYDYAMFGLSPYGPH 134
           GP+F ++ G+ +ALVV++ EIA+E FTTNDR F +RP  VA+K++GY+Y M G++PYGP+
Sbjct: 74  GPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVMLGVAPYGPY 133

Query: 135 WRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLC-VENEEKAVVEMKTWFGDVT 194
           WR  R++  LE L+N RL+ L+HV   E+    KELYKL   +N +  +V+MK W  D+T
Sbjct: 134 WRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLWETQNIDFCLVDMKQWLADLT 193

Query: 195 LNTIFRMVVGKRF----STAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQFLSWFDLGG 254
           LN   +MVVGKRF    S +  +   S  C K LR+ F L G F+ SD   +L W DLGG
Sbjct: 194 LNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYLPYLRWLDLGG 253

Query: 255 HEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKNEAESFSYDA 314
           HEK MK T + LD  F  +L EH++++ S  G  E ++QDFMDVM+S++  E      D 
Sbjct: 254 HEKEMKRTVKELDILFKGWLDEHKRKRLS--GGKEDDDQDFMDVMLSIL--EESKLGNDV 313

Query: 315 ETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRERQVNESDVK 374
           +T+ K  CL +ILGG DTT  T+TWA+SLLLNN  ALKKAQ ELD  VGR+R V+ESD+ 
Sbjct: 314 DTINKTACLAIILGGADTTWATLTWALSLLLNNPNALKKAQDELDLHVGRDRNVDESDLV 373

Query: 375 NLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKLQRDPFVWED 434
            L Y+ AI+KETLRLYPP PLL P    EDCT  GY++  GTRLIVN  K+QRDP VW  
Sbjct: 374 KLTYIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVNAWKIQRDPLVWSQ 433

Query: 435 PCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLANLLHGFEIGR 494
           P E++PERFL  + + D++GQ  + IPFG+GRR CP IS ALQV+ LTLA++LHGFE+  
Sbjct: 434 PHEYQPERFL--ERDVDMKGQHFELIPFGSGRRACPAISLALQVLPLTLAHILHGFELRT 493

Query: 495 PSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVY 525
           P++  +DM E+ G+   + TPLE+++ PR+  + +
Sbjct: 494 PNQNKVDMTETPGIVHAKATPLEVLVAPRISPKCF 518

BLAST of MC11g_new0034 vs. ExPASy Swiss-Prot
Match: O49394 (Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=1 SV=2)

HSP 1 Score: 483.8 bits (1244), Expect = 2.5e-135
Identity = 255/525 (48.57%), Postives = 359/525 (68.38%), Query Frame = 0

Query: 10  IFSGVFALLLFLY-ALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAKEP-THITL 69
           +FS    +L+F++ ALF  S+K      + PAP    GAWP+IGHLH LS KE   + TL
Sbjct: 5   LFSLFVPILVFVFIALFKKSKKPK--HVKAPAP---SGAWPIIGHLHLLSGKEQLLYRTL 64

Query: 70  AKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLGYDYAMFG 129
            KMAD YGP  +LR G  +  VVSS+E+A++ FT ND+  ASRP   A+K +GYD A+FG
Sbjct: 65  GKMADQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFG 124

Query: 130 LSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVE--NEEKAVVEM 189
            +PY   WR+ R++ATLELL+N RL+ L+HVR+ E+   +++LY L V+    E  +V++
Sbjct: 125 FAPYSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVMVDL 184

Query: 190 KTWFGDVTLNTIFRMVVGKRF----STAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQF 249
           K+W  D++LN + RMV GKR+    S + +D   + +C+K + +FF L GIF  SDAF  
Sbjct: 185 KSWLEDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPK 244

Query: 250 LSWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVV-KN 309
           L WFD  GHEK MK+T   LD   +++++ H+QQ+      ++  + DF+DVM+S+  + 
Sbjct: 245 LGWFDFQGHEKEMKQTGRELDVILERWIENHRQQR--KVSGTKHNDSDFVDVMLSLAEQG 304

Query: 310 EAESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRE 369
           +     +DA T IK+TCL +ILGG +T+  T+TWAISLLLNN++ LKKAQ E+D  VGR+
Sbjct: 305 KFSHLQHDAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRD 364

Query: 370 RQVNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKL 429
           R V +SD++NL+Y++AI+KETLRLYP  PLL   E+IEDCT  GY +  GTR++VNV K+
Sbjct: 365 RNVEDSDIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKI 424

Query: 430 QRDPFVWEDPCEFRPERFLTSQ-NNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLA 489
           QRDP V+ +P EFRPERF+T +   FDVRGQ+ + +PFG+GRR CPG S A+QV+HL LA
Sbjct: 425 QRDPRVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLA 484

Query: 490 NLLHGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVY 525
             L  F++    +  +DM ES GLT  + TPLEI+++PRL   +Y
Sbjct: 485 RFLQSFDVKTVMDMPVDMTESPGLTIPKATPLEILISPRLKEGLY 522

BLAST of MC11g_new0034 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 3.6e-134
Identity = 248/525 (47.24%), Postives = 350/525 (66.67%), Query Frame = 0

Query: 7   VLPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAKEPTHIT 66
           +  I S +F L LFLY      RK+S    R    P   GAWP++GHL  L+  +  H  
Sbjct: 15  IASILSLIF-LCLFLY------RKNS----RGKDAPVVSGAWPILGHLSLLNGSQTPHKV 74

Query: 67  LAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLGYDYAMF 126
           L  +AD YGP+FT++ GMK ALV+S+WE+++E FTTND   +SRPKLVA +++ Y+ A  
Sbjct: 75  LGALADKYGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFV 134

Query: 127 GLSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCV---ENEEK-AV 186
           GL+PYGP+WR+ R++ T E L+N R+E+  H+R+ EV+  IKEL+ +     +NE +  +
Sbjct: 135 GLAPYGPYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYTL 194

Query: 187 VEMKTWFGDVTLNTIFRMVVGKRFSTAF--DDNNGSEECKKALRDFFGLFGIFIPSDAFQ 246
           V++K W   +T N + RMVVGKR+      +  + ++   K +R+F  L G F  +D   
Sbjct: 195 VDIKQWLAYLTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGVP 254

Query: 247 FLSWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKN 306
            L W DLGGHEKAMK  A+ +D    ++L+EH+Q++    GE+   ++DFMDVMIS + N
Sbjct: 255 CLRWLDLGGHEKAMKANAKEVDKLLSEWLEEHRQKKL--LGENVESDRDFMDVMISAL-N 314

Query: 307 EAESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRE 366
            A+  ++DA+T+ KAT L +ILGG D+T VT+TWA+SLLL N  AL KA+ E+D Q+G++
Sbjct: 315 GAQIGAFDADTICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGKD 374

Query: 367 RQVNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKL 426
             + ESD+  L+YL+AIVKETLRLYPPAP  +P E  E+C   GY+I  GTRLI N+ K+
Sbjct: 375 EYIRESDISKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWKI 434

Query: 427 QRDPFVWEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLAN 486
            RDP VW DP EF+PERFLT+  + D+RG + + +PFG+GRR+C G+S  L ++H TLAN
Sbjct: 435 HRDPSVWSDPLEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTLAN 494

Query: 487 LLHGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVYE 526
           LLH F+I  PS E +DM E  G T+ + TPLEI++ PR     YE
Sbjct: 495 LLHSFDILNPSAEPVDMTEFFGFTNTKATPLEILVKPRQSPNYYE 525

BLAST of MC11g_new0034 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 1.1e-130
Identity = 245/519 (47.21%), Postives = 342/519 (65.90%), Query Frame = 0

Query: 10  IFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAKEPTHITLAK 69
           + S +F L LFLY        SS +       P  GGAWP+ GHL  L   +  H  L  
Sbjct: 15  VVSLIFLLCLFLYGPLKKVHGSSKE------APTVGGAWPIFGHLPLLIGSKSPHKALGA 74

Query: 70  MADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLGYDYAMFGLS 129
           +A+ +GP+FT++ G KKALVVS WE+ARE FTTND   ++RPKL+ ++L+ Y+ AM  ++
Sbjct: 75  LAEKHGPLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMCYNNAMLLVA 134

Query: 130 PYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKL--CVENE-EKAVVEMK 189
           PYGP+WR+ R++   E+L++ R+E+LQ VR+ EVQN I ELY +    +NE + A VE+K
Sbjct: 135 PYGPYWRELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNESDYASVELK 194

Query: 190 TWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQFLSWFD 249
            WF     N + RMVVGKRF +A   +  +E+C KA+ +F  L G+F   DA  +L W D
Sbjct: 195 QWFAQPIFNMVLRMVVGKRFLSATATDEKAEKCVKAVDEFMRLAGVFTVGDAIPYLRWLD 254

Query: 250 LGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKNEAESFS 309
            GG+EKAMKETA+ LD    ++L+EH+Q++A   GE     QDFM+VM+S +  +     
Sbjct: 255 FGGYEKAMKETAKELDVMISEWLEEHRQKRA--LGEGVDGAQDFMNVMLSSLDGKTID-G 314

Query: 310 YDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRERQVNES 369
            DA+T+IK+T L +I  G + +  T+ WA+ L+L N   L+  + ELD QVG++R + ES
Sbjct: 315 IDADTLIKSTVLTIIQAGTEASISTIIWAMCLILKNPLILEN-KAELDIQVGKDRCICES 374

Query: 370 DVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKLQRDPFV 429
           D+ NL+YL+A+VKETLRLY P PL +P E  EDCT  GY++  GTRLI N+ K+  DP V
Sbjct: 375 DISNLVYLQAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRLITNIWKIHTDPNV 434

Query: 430 WEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLANLLHGFE 489
           W DP EF+P+RFLT+  + DV+G   Q +PFG+GRR+CPGISF LQ +HL LA+ LH FE
Sbjct: 435 WSDPFEFKPDRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQTVHLALASFLHSFE 494

Query: 490 IGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVYE 526
           I  PS E LDM E+ G+T+ + TPLE+++ P L    Y+
Sbjct: 495 ILNPSTEPLDMTEAFGVTNTKATPLEVLVKPCLSPSCYK 523

BLAST of MC11g_new0034 vs. NCBI nr
Match: XP_022138438.1 (cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 1046 bits (2704), Expect = 0.0
Identity = 525/525 (100.00%), Postives = 525/525 (100.00%), Query Frame = 0

Query: 1   MEVTGHVLPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAK 60
           MEVTGHVLPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAK
Sbjct: 1   MEVTGHVLPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAK 60

Query: 61  EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLG 120
           EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLG
Sbjct: 61  EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLG 120

Query: 121 YDYAMFGLSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVENEEK 180
           YDYAMFGLSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVENEEK
Sbjct: 121 YDYAMFGLSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVENEEK 180

Query: 181 AVVEMKTWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQ 240
           AVVEMKTWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQ
Sbjct: 181 AVVEMKTWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQ 240

Query: 241 FLSWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKN 300
           FLSWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKN
Sbjct: 241 FLSWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKN 300

Query: 301 EAESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRE 360
           EAESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRE
Sbjct: 301 EAESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRE 360

Query: 361 RQVNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKL 420
           RQVNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKL
Sbjct: 361 RQVNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKL 420

Query: 421 QRDPFVWEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLAN 480
           QRDPFVWEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLAN
Sbjct: 421 QRDPFVWEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLAN 480

Query: 481 LLHGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVYE 525
           LLHGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVYE
Sbjct: 481 LLHGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVYE 525

BLAST of MC11g_new0034 vs. NCBI nr
Match: XP_022138580.1 (cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 880 bits (2273), Expect = 0.0
Identity = 450/524 (85.88%), Postives = 475/524 (90.65%), Query Frame = 0

Query: 1   MEVTGHVLPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAK 60
           MEV  HV  IF+G+F LL FLYALF MSRKS ADRRRLP PPEAGGA PVIGHLH LSAK
Sbjct: 39  MEVP-HV-SIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAK 98

Query: 61  EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLG 120
           EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDR+FASRPKLVASKLLG
Sbjct: 99  EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLG 158

Query: 121 YDYAMFGLSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVENEEK 180
           YDYAMFGLSPYGPHWRQARRVATLELLTNHRLE+LQHVRILEV+NWIKELYKLCV++EEK
Sbjct: 159 YDYAMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEK 218

Query: 181 AVVEMKTWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQ 240
           AVVEMK WFGD+TL+TIFR+VVGKRFS AF+  +GSEECKKALRDFF LFGIFIPSD+F 
Sbjct: 219 AVVEMKKWFGDLTLDTIFRIVVGKRFSAAFN-GSGSEECKKALRDFFELFGIFIPSDSFP 278

Query: 241 FLSWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKN 300
           FLSW DLGGHEKAMK TA+LLD  FDKFLK+H Q   +NF  SE  +QDFM+VMIS VKN
Sbjct: 279 FLSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQ--TTNFAASEEADQDFMEVMISAVKN 338

Query: 301 EAESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRE 360
           E ESFSYDA TVIKATCLNMILGGFDTTTVTMTW ISLLLNNQE L+KAQLELDEQVGR 
Sbjct: 339 EGESFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRG 398

Query: 361 RQVNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKL 420
           R+V ESDVKNLLYL+AIVKETLRLYP APLL PHESIEDCTA GY+IP GTRLIVNV+KL
Sbjct: 399 REVKESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKL 458

Query: 421 QRDPFVWEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLAN 480
           QRDP VWED CEFRPERFLTSQ NFDVRGQSPQ IPF NGRRMCPGISFALQVIHLTLAN
Sbjct: 459 QRDPLVWEDSCEFRPERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLAN 518

Query: 481 LLHGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVY 524
           LLHGF+I RPSEE LDMEESVGLTSVRK PLE+VLTP LPAQVY
Sbjct: 519 LLHGFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 557

BLAST of MC11g_new0034 vs. NCBI nr
Match: XP_022940485.1 (cytochrome P450 CYP82D47-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 764 bits (1973), Expect = 6.71e-274
Identity = 382/519 (73.60%), Postives = 442/519 (85.16%), Query Frame = 0

Query: 8   LPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAKEPTHITL 67
           LP+   +FALL FLYALFT+SR+S+A R++LP  PE GGAWPVIGHLH LS KEPTHITL
Sbjct: 4   LPLAGIIFALLFFLYALFTISRRSAAHRKKLP--PECGGAWPVIGHLHLLSTKEPTHITL 63

Query: 68  AKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLGYDYAMFG 127
           +K+AD YGP+FT RFGMKKAL+VS+WEIA+E FTT DR+FASRPKL AS LLGYDYAM G
Sbjct: 64  SKLADKYGPIFTFRFGMKKALIVSNWEIAKEVFTTKDRIFASRPKLAASNLLGYDYAMMG 123

Query: 128 LSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVEN-EEKAVVEMK 187
           LSPY PHWR AR++ATLELLTNHRLE+LQH+R  EVQ W  +LY+L V+  +EK VVEMK
Sbjct: 124 LSPYSPHWRHARKIATLELLTNHRLEQLQHIRAAEVQRWNNKLYELWVKRKDEKLVVEMK 183

Query: 188 TWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQFLSWFD 247
             F D+TLNTIF MV+GKRFSTAFD ++G E+C+KALRD F LFG+F+PSD+F FL W D
Sbjct: 184 NLFADITLNTIFMMVIGKRFSTAFDGHDG-EDCQKALRDVFELFGMFVPSDSFPFLWWLD 243

Query: 248 LGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKNEAESFS 307
            GGH+K MK+TA++LD  F+KFLKEH+Q++  +  + EM+E+DFMDVMIS V  +   FS
Sbjct: 244 FGGHQKRMKKTAKILDGAFEKFLKEHRQRR--DRVKLEMKEEDFMDVMISRVNEDGNLFS 303

Query: 308 YDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRERQVNES 367
           +D +TV+KATCLNMILGGFDT TVTMTWA+SLLLNN+E+LKKAQLELDEQVGRERQV ES
Sbjct: 304 FDGDTVVKATCLNMILGGFDTMTVTMTWALSLLLNNEESLKKAQLELDEQVGRERQVQES 363

Query: 368 DVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKLQRDPFV 427
           DVKNL YL+AIVKETLRLYP APLL PHES+EDCT  GYYIP GTRLIV+ +KLQ+DP  
Sbjct: 364 DVKNLPYLQAIVKETLRLYPAAPLLVPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDPSF 423

Query: 428 WEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLANLLHGFE 487
           WE+P EFRP+RFLTSQ N DVRGQSPQ IPFGNGRRMCPGISFALQV+HLTL NLLHGF 
Sbjct: 424 WENPYEFRPKRFLTSQKNVDVRGQSPQLIPFGNGRRMCPGISFALQVMHLTLVNLLHGFN 483

Query: 488 IGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVYE 525
           IGR SEE LDMEES GLTSVRK PLE+VL+PRLPA VYE
Sbjct: 484 IGRMSEEPLDMEESFGLTSVRKAPLEVVLSPRLPAHVYE 517

BLAST of MC11g_new0034 vs. NCBI nr
Match: XP_022940488.1 (cytochrome P450 CYP82D47-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 762 bits (1968), Expect = 3.87e-273
Identity = 381/518 (73.55%), Postives = 441/518 (85.14%), Query Frame = 0

Query: 8   LPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAKEPTHITL 67
           LP+   +FALL FLYALFT+SR+S+A R++LP  PE GGAWPVIGHLH LS KEPTHITL
Sbjct: 4   LPLAGIIFALLFFLYALFTISRRSAAHRKKLP--PECGGAWPVIGHLHLLSTKEPTHITL 63

Query: 68  AKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLGYDYAMFG 127
           +K+AD YGP+FT RFGMKKAL+VS+WEIA+E FTT DR+FASRPKL AS LLGYDYAM G
Sbjct: 64  SKLADKYGPIFTFRFGMKKALIVSNWEIAKEVFTTKDRIFASRPKLAASNLLGYDYAMMG 123

Query: 128 LSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVEN-EEKAVVEMK 187
           LSPY PHWR AR++ATLELLTNHRLE+LQH+R  EVQ W  +LY+L V+  +EK VVEMK
Sbjct: 124 LSPYSPHWRHARKIATLELLTNHRLEQLQHIRAAEVQRWNNKLYELWVKRKDEKLVVEMK 183

Query: 188 TWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQFLSWFD 247
             F D+TLNTIF MV+GKRFSTAFD ++G E+C+KALRD F LFG+F+PSD+F FL W D
Sbjct: 184 NLFADITLNTIFMMVIGKRFSTAFDGHDG-EDCQKALRDVFELFGMFVPSDSFPFLWWLD 243

Query: 248 LGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKNEAESFS 307
            GGH+K MK+TA++LD  F+KFLKEH+Q++  +  + EM+E+DFMDVMIS V  +   FS
Sbjct: 244 FGGHQKRMKKTAKILDGAFEKFLKEHRQRR--DRVKLEMKEEDFMDVMISRVNEDGNLFS 303

Query: 308 YDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRERQVNES 367
           +D +TV+KATCLNMILGGFDT TVTMTWA+SLLLNN+E+LKKAQLELDEQVGRERQV ES
Sbjct: 304 FDGDTVVKATCLNMILGGFDTMTVTMTWALSLLLNNEESLKKAQLELDEQVGRERQVQES 363

Query: 368 DVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKLQRDPFV 427
           DVKNL YL+AIVKETLRLYP APLL PHES+EDCT  GYYIP GTRLIV+ +KLQ+DP  
Sbjct: 364 DVKNLPYLQAIVKETLRLYPAAPLLVPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDPSF 423

Query: 428 WEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLANLLHGFE 487
           WE+P EFRP+RFLTSQ N DVRGQSPQ IPFGNGRRMCPGISFALQV+HLTL NLLHGF 
Sbjct: 424 WENPYEFRPKRFLTSQKNVDVRGQSPQLIPFGNGRRMCPGISFALQVMHLTLVNLLHGFN 483

Query: 488 IGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVY 524
           IGR SEE LDMEES GLTSVRK PLE+VL+PRLPA VY
Sbjct: 484 IGRMSEEPLDMEESFGLTSVRKAPLEVVLSPRLPAHVY 516

BLAST of MC11g_new0034 vs. NCBI nr
Match: XP_023535449.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 761 bits (1964), Expect = 1.63e-272
Identity = 380/523 (72.66%), Postives = 436/523 (83.37%), Query Frame = 0

Query: 6   HVLPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAKEPTHI 65
           H+ P+   +F  LLFLY  FT SR+S A R+R+P  PEAGGAWPVIGHLH L A EPTHI
Sbjct: 3   HLRPLAGIIFPFLLFLYVFFTWSRRSVAHRKRIP--PEAGGAWPVIGHLHLLHAGEPTHI 62

Query: 66  TLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLGYDYAM 125
           TLAKMADAYGP+FT RFGMKKAL+VSSWEIA+EFFTTNDR+FASRPKLVASKLL YDYAM
Sbjct: 63  TLAKMADAYGPIFTFRFGMKKALIVSSWEIAKEFFTTNDRIFASRPKLVASKLLAYDYAM 122

Query: 126 FGLSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCV---ENEEKAV 185
            G SPY PHWRQ R++ATLELLTNHRLE+LQH+R  EVQ W+ EL+K+ +   E  EK  
Sbjct: 123 IGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRAFEVQQWMNELHKMSIKSNERGEKVA 182

Query: 186 VEMKTWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQFL 245
           VEMK W  D+TLNTIF+MV+GKRFS      +G+E   KAL DFFGLF IF+PSD+F FL
Sbjct: 183 VEMKKWLADITLNTIFKMVIGKRFSAI---QHGNENFPKALIDFFGLFHIFVPSDSFPFL 242

Query: 246 SWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKNEA 305
           SW DLGGHEK MK+TA+++D+ F KFL++H++++  N  E+E  EQDFMDVMIS V+ + 
Sbjct: 243 SWLDLGGHEKTMKKTAKVIDEAFHKFLQQHRERR--NDAEAETAEQDFMDVMISRVEEDG 302

Query: 306 ESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRERQ 365
           + FSYDA+TVIKAT LNM+L GFDTTT+TM WA+SLLLNN+  L+ AQLELDE+VGR RQ
Sbjct: 303 QHFSYDADTVIKATSLNMVLAGFDTTTITMVWALSLLLNNEGVLENAQLELDEKVGRSRQ 362

Query: 366 VNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKLQR 425
           V ESDVKNL YLRAIVKETLRLYP APLL PHESIEDCTA GY+I  GTRLIVNV+KLQ+
Sbjct: 363 VKESDVKNLPYLRAIVKETLRLYPAAPLLVPHESIEDCTAAGYHISKGTRLIVNVQKLQK 422

Query: 426 DPFVWEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLANLL 485
           DP VWE PCEFRPERFLTSQ +FDVRGQSPQFIPFGNGRRMCP ISFALQ+IHLTLAN L
Sbjct: 423 DPLVWEGPCEFRPERFLTSQKDFDVRGQSPQFIPFGNGRRMCPAISFALQMIHLTLANFL 482

Query: 486 HGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVYE 525
           HGF+I R S+E LDMEESVGLTS+RK PLE+VLTPRLPA VYE
Sbjct: 483 HGFKIERSSDEPLDMEESVGLTSLRKAPLEVVLTPRLPAHVYE 518

BLAST of MC11g_new0034 vs. ExPASy TrEMBL
Match: A0A6J1CCZ4 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009608 PE=3 SV=1)

HSP 1 Score: 1046 bits (2704), Expect = 0.0
Identity = 525/525 (100.00%), Postives = 525/525 (100.00%), Query Frame = 0

Query: 1   MEVTGHVLPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAK 60
           MEVTGHVLPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAK
Sbjct: 1   MEVTGHVLPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAK 60

Query: 61  EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLG 120
           EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLG
Sbjct: 61  EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLG 120

Query: 121 YDYAMFGLSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVENEEK 180
           YDYAMFGLSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVENEEK
Sbjct: 121 YDYAMFGLSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVENEEK 180

Query: 181 AVVEMKTWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQ 240
           AVVEMKTWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQ
Sbjct: 181 AVVEMKTWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQ 240

Query: 241 FLSWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKN 300
           FLSWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKN
Sbjct: 241 FLSWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKN 300

Query: 301 EAESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRE 360
           EAESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRE
Sbjct: 301 EAESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRE 360

Query: 361 RQVNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKL 420
           RQVNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKL
Sbjct: 361 RQVNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKL 420

Query: 421 QRDPFVWEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLAN 480
           QRDPFVWEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLAN
Sbjct: 421 QRDPFVWEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLAN 480

Query: 481 LLHGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVYE 525
           LLHGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVYE
Sbjct: 481 LLHGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVYE 525

BLAST of MC11g_new0034 vs. ExPASy TrEMBL
Match: A0A6J1C9U5 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111009700 PE=3 SV=1)

HSP 1 Score: 880 bits (2273), Expect = 0.0
Identity = 450/524 (85.88%), Postives = 475/524 (90.65%), Query Frame = 0

Query: 1   MEVTGHVLPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAK 60
           MEV  HV  IF+G+F LL FLYALF MSRKS ADRRRLP PPEAGGA PVIGHLH LSAK
Sbjct: 39  MEVP-HV-SIFTGIFVLLFFLYALFNMSRKSGADRRRLPVPPEAGGALPVIGHLHLLSAK 98

Query: 61  EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLG 120
           EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDR+FASRPKLVASKLLG
Sbjct: 99  EPTHITLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRLFASRPKLVASKLLG 158

Query: 121 YDYAMFGLSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVENEEK 180
           YDYAMFGLSPYGPHWRQARRVATLELLTNHRLE+LQHVRILEV+NWIKELYKLCV++EEK
Sbjct: 159 YDYAMFGLSPYGPHWRQARRVATLELLTNHRLEKLQHVRILEVKNWIKELYKLCVKSEEK 218

Query: 181 AVVEMKTWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQ 240
           AVVEMK WFGD+TL+TIFR+VVGKRFS AF+  +GSEECKKALRDFF LFGIFIPSD+F 
Sbjct: 219 AVVEMKKWFGDLTLDTIFRIVVGKRFSAAFN-GSGSEECKKALRDFFELFGIFIPSDSFP 278

Query: 241 FLSWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKN 300
           FLSW DLGGHEKAMK TA+LLD  FDKFLK+H Q   +NF  SE  +QDFM+VMIS VKN
Sbjct: 279 FLSWLDLGGHEKAMKHTAKLLDHAFDKFLKDHNQ--TTNFAASEEADQDFMEVMISAVKN 338

Query: 301 EAESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRE 360
           E ESFSYDA TVIKATCLNMILGGFDTTTVTMTW ISLLLNNQE L+KAQLELDEQVGR 
Sbjct: 339 EGESFSYDANTVIKATCLNMILGGFDTTTVTMTWVISLLLNNQETLQKAQLELDEQVGRG 398

Query: 361 RQVNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKL 420
           R+V ESDVKNLLYL+AIVKETLRLYP APLL PHESIEDCTA GY+IP GTRLIVNV+KL
Sbjct: 399 REVKESDVKNLLYLQAIVKETLRLYPAAPLLVPHESIEDCTAAGYHIPIGTRLIVNVQKL 458

Query: 421 QRDPFVWEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLAN 480
           QRDP VWED CEFRPERFLTSQ NFDVRGQSPQ IPF NGRRMCPGISFALQVIHLTLAN
Sbjct: 459 QRDPLVWEDSCEFRPERFLTSQKNFDVRGQSPQLIPFRNGRRMCPGISFALQVIHLTLAN 518

Query: 481 LLHGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVY 524
           LLHGF+I RPSEE LDMEESVGLTSVRK PLE+VLTP LPAQVY
Sbjct: 519 LLHGFKIDRPSEEPLDMEESVGLTSVRKAPLEVVLTPCLPAQVY 557

BLAST of MC11g_new0034 vs. ExPASy TrEMBL
Match: A0A6J1FQP6 (cytochrome P450 CYP82D47-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446069 PE=3 SV=1)

HSP 1 Score: 764 bits (1973), Expect = 3.25e-274
Identity = 382/519 (73.60%), Postives = 442/519 (85.16%), Query Frame = 0

Query: 8   LPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAKEPTHITL 67
           LP+   +FALL FLYALFT+SR+S+A R++LP  PE GGAWPVIGHLH LS KEPTHITL
Sbjct: 4   LPLAGIIFALLFFLYALFTISRRSAAHRKKLP--PECGGAWPVIGHLHLLSTKEPTHITL 63

Query: 68  AKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLGYDYAMFG 127
           +K+AD YGP+FT RFGMKKAL+VS+WEIA+E FTT DR+FASRPKL AS LLGYDYAM G
Sbjct: 64  SKLADKYGPIFTFRFGMKKALIVSNWEIAKEVFTTKDRIFASRPKLAASNLLGYDYAMMG 123

Query: 128 LSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVEN-EEKAVVEMK 187
           LSPY PHWR AR++ATLELLTNHRLE+LQH+R  EVQ W  +LY+L V+  +EK VVEMK
Sbjct: 124 LSPYSPHWRHARKIATLELLTNHRLEQLQHIRAAEVQRWNNKLYELWVKRKDEKLVVEMK 183

Query: 188 TWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQFLSWFD 247
             F D+TLNTIF MV+GKRFSTAFD ++G E+C+KALRD F LFG+F+PSD+F FL W D
Sbjct: 184 NLFADITLNTIFMMVIGKRFSTAFDGHDG-EDCQKALRDVFELFGMFVPSDSFPFLWWLD 243

Query: 248 LGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKNEAESFS 307
            GGH+K MK+TA++LD  F+KFLKEH+Q++  +  + EM+E+DFMDVMIS V  +   FS
Sbjct: 244 FGGHQKRMKKTAKILDGAFEKFLKEHRQRR--DRVKLEMKEEDFMDVMISRVNEDGNLFS 303

Query: 308 YDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRERQVNES 367
           +D +TV+KATCLNMILGGFDT TVTMTWA+SLLLNN+E+LKKAQLELDEQVGRERQV ES
Sbjct: 304 FDGDTVVKATCLNMILGGFDTMTVTMTWALSLLLNNEESLKKAQLELDEQVGRERQVQES 363

Query: 368 DVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKLQRDPFV 427
           DVKNL YL+AIVKETLRLYP APLL PHES+EDCT  GYYIP GTRLIV+ +KLQ+DP  
Sbjct: 364 DVKNLPYLQAIVKETLRLYPAAPLLVPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDPSF 423

Query: 428 WEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLANLLHGFE 487
           WE+P EFRP+RFLTSQ N DVRGQSPQ IPFGNGRRMCPGISFALQV+HLTL NLLHGF 
Sbjct: 424 WENPYEFRPKRFLTSQKNVDVRGQSPQLIPFGNGRRMCPGISFALQVMHLTLVNLLHGFN 483

Query: 488 IGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVYE 525
           IGR SEE LDMEES GLTSVRK PLE+VL+PRLPA VYE
Sbjct: 484 IGRMSEEPLDMEESFGLTSVRKAPLEVVLSPRLPAHVYE 517

BLAST of MC11g_new0034 vs. ExPASy TrEMBL
Match: A0A6J1FPG1 (cytochrome P450 CYP82D47-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446069 PE=3 SV=1)

HSP 1 Score: 762 bits (1968), Expect = 1.87e-273
Identity = 381/518 (73.55%), Postives = 441/518 (85.14%), Query Frame = 0

Query: 8   LPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAKEPTHITL 67
           LP+   +FALL FLYALFT+SR+S+A R++LP  PE GGAWPVIGHLH LS KEPTHITL
Sbjct: 4   LPLAGIIFALLFFLYALFTISRRSAAHRKKLP--PECGGAWPVIGHLHLLSTKEPTHITL 63

Query: 68  AKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLGYDYAMFG 127
           +K+AD YGP+FT RFGMKKAL+VS+WEIA+E FTT DR+FASRPKL AS LLGYDYAM G
Sbjct: 64  SKLADKYGPIFTFRFGMKKALIVSNWEIAKEVFTTKDRIFASRPKLAASNLLGYDYAMMG 123

Query: 128 LSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVEN-EEKAVVEMK 187
           LSPY PHWR AR++ATLELLTNHRLE+LQH+R  EVQ W  +LY+L V+  +EK VVEMK
Sbjct: 124 LSPYSPHWRHARKIATLELLTNHRLEQLQHIRAAEVQRWNNKLYELWVKRKDEKLVVEMK 183

Query: 188 TWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQFLSWFD 247
             F D+TLNTIF MV+GKRFSTAFD ++G E+C+KALRD F LFG+F+PSD+F FL W D
Sbjct: 184 NLFADITLNTIFMMVIGKRFSTAFDGHDG-EDCQKALRDVFELFGMFVPSDSFPFLWWLD 243

Query: 248 LGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKNEAESFS 307
            GGH+K MK+TA++LD  F+KFLKEH+Q++  +  + EM+E+DFMDVMIS V  +   FS
Sbjct: 244 FGGHQKRMKKTAKILDGAFEKFLKEHRQRR--DRVKLEMKEEDFMDVMISRVNEDGNLFS 303

Query: 308 YDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRERQVNES 367
           +D +TV+KATCLNMILGGFDT TVTMTWA+SLLLNN+E+LKKAQLELDEQVGRERQV ES
Sbjct: 304 FDGDTVVKATCLNMILGGFDTMTVTMTWALSLLLNNEESLKKAQLELDEQVGRERQVQES 363

Query: 368 DVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKLQRDPFV 427
           DVKNL YL+AIVKETLRLYP APLL PHES+EDCT  GYYIP GTRLIV+ +KLQ+DP  
Sbjct: 364 DVKNLPYLQAIVKETLRLYPAAPLLVPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDPSF 423

Query: 428 WEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLANLLHGFE 487
           WE+P EFRP+RFLTSQ N DVRGQSPQ IPFGNGRRMCPGISFALQV+HLTL NLLHGF 
Sbjct: 424 WENPYEFRPKRFLTSQKNVDVRGQSPQLIPFGNGRRMCPGISFALQVMHLTLVNLLHGFN 483

Query: 488 IGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVY 524
           IGR SEE LDMEES GLTSVRK PLE+VL+PRLPA VY
Sbjct: 484 IGRMSEEPLDMEESFGLTSVRKAPLEVVLSPRLPAHVY 516

BLAST of MC11g_new0034 vs. ExPASy TrEMBL
Match: A0A6J1IFL6 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111476811 PE=3 SV=1)

HSP 1 Score: 756 bits (1952), Expect = 6.83e-271
Identity = 375/523 (71.70%), Postives = 437/523 (83.56%), Query Frame = 0

Query: 6   HVLPIFSGVFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAKEPTHI 65
           H+  +   +F  L F+Y LFT SR+S A R+R+P  PEAGGAWPVIGHLH L A +PTHI
Sbjct: 3   HLYALAGIIFPFLFFVYVLFTSSRRSVAHRKRIP--PEAGGAWPVIGHLHLLHAGDPTHI 62

Query: 66  TLAKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLGYDYAM 125
            LAKMADAYGPMFTLRFGMKKALVVSSWEIA+EF TTNDR+FASRPKLVASKLL YDYAM
Sbjct: 63  ALAKMADAYGPMFTLRFGMKKALVVSSWEIAKEFLTTNDRIFASRPKLVASKLLAYDYAM 122

Query: 126 FGLSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVENEE---KAV 185
            G SPY PHWRQ R++ATLELLTNHRLE+LQH+R+ EVQ W+KEL+K+ + + E   K  
Sbjct: 123 MGFSPYTPHWRQVRKIATLELLTNHRLEQLQHIRVFEVQRWMKELHKMSIRSNERGDKVA 182

Query: 186 VEMKTWFGDVTLNTIFRMVVGKRFSTAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQFL 245
           VEMK W  D+TLNTIF+MV+GKRFS      +G+E   KAL DFFGLF IF+PSD+F FL
Sbjct: 183 VEMKKWLADITLNTIFKMVIGKRFSAI---QHGTENFPKALIDFFGLFHIFVPSDSFPFL 242

Query: 246 SWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKNEA 305
           SW DLGGHEK MK+TA+++D+ F KFL++H++++  N G++E  EQDFMDVMIS V+ + 
Sbjct: 243 SWLDLGGHEKTMKKTAKVIDEAFHKFLQQHRERR--NEGQAETAEQDFMDVMISRVEEDE 302

Query: 306 ESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRERQ 365
           + FSYDA+TVIKATCLNMI+GGFDTT VTMTWA+ LLLNN+EALKK QLELDE+VGR  Q
Sbjct: 303 QHFSYDADTVIKATCLNMIVGGFDTTAVTMTWALCLLLNNEEALKKVQLELDEKVGRSTQ 362

Query: 366 VNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKLQR 425
           V ESDVKNL YL+AIVKETLRLYP  PL+APHES+EDCT  GY+I  GTRLIVNV+KLQ+
Sbjct: 363 VKESDVKNLPYLQAIVKETLRLYPAVPLMAPHESVEDCTVAGYHICKGTRLIVNVQKLQK 422

Query: 426 DPFVWEDPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLANLL 485
           DP VWE+P EF PERF+TSQ NFDVRG SPQFIPFGNGRRMCP ISFALQV+H+TLAN L
Sbjct: 423 DPRVWEEPNEFCPERFVTSQKNFDVRGLSPQFIPFGNGRRMCPAISFALQVMHITLANFL 482

Query: 486 HGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVYE 525
           HGFEI RPSEELLDMEE +GLTS+RK PLE+VLTPRLPA +YE
Sbjct: 483 HGFEIERPSEELLDMEEGMGLTSLRKAPLEVVLTPRLPAHIYE 518

BLAST of MC11g_new0034 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 508.1 bits (1307), Expect = 8.7e-144
Identity = 263/526 (50.00%), Postives = 368/526 (69.96%), Query Frame = 0

Query: 10  IFSGVFALLLFLY-ALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAKEP-THITL 69
           +FS    +L+F++ ALF  S+K      + PAP    GAWP+IGHLH L  KE   + TL
Sbjct: 5   LFSLFVPILVFVFIALFKKSKKPK--YVKAPAP---SGAWPIIGHLHLLGGKEQLLYRTL 64

Query: 70  AKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLGYDYAMFG 129
            KMAD YGP  +L+ G  +A VVSS+E+A++ FT ND+  ASRP   A+K +GY++A+FG
Sbjct: 65  GKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFG 124

Query: 130 LSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVEN--EEKAVVEM 189
            +PY   WR+ R++AT+ELL+N RL+ L+HVR+ E+   +K+LY L  +N   +  +V++
Sbjct: 125 FAPYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVDL 184

Query: 190 KTWFGDVTLNTIFRMVVGKRF-----STAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQ 249
           K+W  D+TLN I RMV GKR+     S + +D   + +CKKA+  FF L GIF  SDAF 
Sbjct: 185 KSWLEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFP 244

Query: 250 FLSWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVV-K 309
            LS+FDL GHEK MK+T   LD   +++++ H+QQ+   F  ++  + DF+DVM+S+  +
Sbjct: 245 TLSFFDLQGHEKEMKQTGSELDVILERWIENHRQQR--KFSGTKENDSDFIDVMMSLAEQ 304

Query: 310 NEAESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGR 369
            +     YDA T IK+TCL +ILGG DT+  T+TWAISLLLNN+E LKKAQ E+D  VGR
Sbjct: 305 GKLSHLQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGR 364

Query: 370 ERQVNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKK 429
           +R V +SD++NL+YL+AI+KETLRLYP  PLL P E++EDCT  GYY+P GTRLIVNV K
Sbjct: 365 DRNVEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWK 424

Query: 430 LQRDPFVWEDPCEFRPERFLTSQ-NNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTL 489
           +QRDP V+ +P EFRPERF+T +   FDVRGQ+ + +PFG+GRR CPG S A+QV+HL L
Sbjct: 425 IQRDPKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGL 484

Query: 490 ANLLHGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVY 525
           A  LH F++    +  +DM E+ GLT  + TPLE++++PR+  +++
Sbjct: 485 ARFLHSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRIKEELF 523

BLAST of MC11g_new0034 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 483.8 bits (1244), Expect = 1.8e-136
Identity = 255/525 (48.57%), Postives = 359/525 (68.38%), Query Frame = 0

Query: 10  IFSGVFALLLFLY-ALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAKEP-THITL 69
           +FS    +L+F++ ALF  S+K      + PAP    GAWP+IGHLH LS KE   + TL
Sbjct: 5   LFSLFVPILVFVFIALFKKSKKPK--HVKAPAP---SGAWPIIGHLHLLSGKEQLLYRTL 64

Query: 70  AKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLGYDYAMFG 129
            KMAD YGP  +LR G  +  VVSS+E+A++ FT ND+  ASRP   A+K +GYD A+FG
Sbjct: 65  GKMADQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFG 124

Query: 130 LSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVE--NEEKAVVEM 189
            +PY   WR+ R++ATLELL+N RL+ L+HVR+ E+   +++LY L V+    E  +V++
Sbjct: 125 FAPYSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVMVDL 184

Query: 190 KTWFGDVTLNTIFRMVVGKRF----STAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQF 249
           K+W  D++LN + RMV GKR+    S + +D   + +C+K + +FF L GIF  SDAF  
Sbjct: 185 KSWLEDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPK 244

Query: 250 LSWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVV-KN 309
           L WFD  GHEK MK+T   LD   +++++ H+QQ+      ++  + DF+DVM+S+  + 
Sbjct: 245 LGWFDFQGHEKEMKQTGRELDVILERWIENHRQQR--KVSGTKHNDSDFVDVMLSLAEQG 304

Query: 310 EAESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRE 369
           +     +DA T IK+TCL +ILGG +T+  T+TWAISLLLNN++ LKKAQ E+D  VGR+
Sbjct: 305 KFSHLQHDAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRD 364

Query: 370 RQVNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKL 429
           R V +SD++NL+Y++AI+KETLRLYP  PLL   E+IEDCT  GY +  GTR++VNV K+
Sbjct: 365 RNVEDSDIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKI 424

Query: 430 QRDPFVWEDPCEFRPERFLTSQ-NNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLA 489
           QRDP V+ +P EFRPERF+T +   FDVRGQ+ + +PFG+GRR CPG S A+QV+HL LA
Sbjct: 425 QRDPRVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLA 484

Query: 490 NLLHGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVY 525
             L  F++    +  +DM ES GLT  + TPLEI+++PRL   +Y
Sbjct: 485 RFLQSFDVKTVMDMPVDMTESPGLTIPKATPLEILISPRLKEGLY 522

BLAST of MC11g_new0034 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 444.5 bits (1142), Expect = 1.2e-124
Identity = 243/526 (46.20%), Postives = 343/526 (65.21%), Query Frame = 0

Query: 10  IFSGVFALLLFLY-ALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAKEP-THITL 69
           +FS   ++L+F++ ALF  S+K      + PAP    GAWP+IGHLH L  KE   + TL
Sbjct: 5   LFSLFVSILVFVFIALFKKSKKPK--YVKAPAP---SGAWPIIGHLHLLGGKEQLLYRTL 64

Query: 70  AKMADAYGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLGYDYAMFG 129
            KMAD YGP  +LR G  +  V SS+E+A++ FT ND+  AS     A+K +GY +    
Sbjct: 65  GKMADHYGPAMSLRLGSSETFVGSSFEVAKDCFTVNDKALASL-MTAAAKHMGYVF---- 124

Query: 130 LSPYGPHWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVE--NEEKAVVEM 189
                  W + R++A +ELL+N RL+ L +VR+ E+   +K+LY L V+    E  +V++
Sbjct: 125 -------WLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGGSEPVMVDL 184

Query: 190 KTWFGDVTLNTIFRMVVGKRF-----STAFDDNNGSEECKKALRDFFGLFGIFIPSDAFQ 249
           K+W  D+  N I RMV GKR+     + + +    + + +K +  FF L GIF  SDAF 
Sbjct: 185 KSWLEDMIANMIMRMVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFP 244

Query: 250 FLSWFDLGGHEKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVV-K 309
            L W DL GHEK MK+T   LD   +++++ H+QQ+      ++  + DF+DVM+S+  +
Sbjct: 245 KLGWLDLQGHEKEMKQTRRELDVILERWIENHRQQR--KVSGTKHNDSDFVDVMLSLAEQ 304

Query: 310 NEAESFSYDAETVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGR 369
            +     YDA T IK TCL +ILGG +T+  T+TWAISLLLNN++ LKK Q E+D  VGR
Sbjct: 305 GKLSHLQYDANTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGR 364

Query: 370 ERQVNESDVKNLLYLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKK 429
           +R V +SD+KNL+YL+AI+KETLRLYP APLL   E++EDCT  GY +P GTRLIVNV K
Sbjct: 365 DRNVEDSDIKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWK 424

Query: 430 LQRDPFVWEDPCEFRPERFLTSQ-NNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTL 489
           +QRDP V+ +P EFRPERF+T +  +FDVRGQ+ + +PFG+GRR CPG S A+Q++HL L
Sbjct: 425 IQRDPKVYMEPNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGL 484

Query: 490 ANLLHGFEIGRPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVY 525
           A  LH FE+    +  +DM ES GLT  + TPLE+++ PRL  +++
Sbjct: 485 ARFLHSFEVKTVLDRPVDMSESPGLTITKATPLEVLINPRLKRELF 511

BLAST of MC11g_new0034 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 382.5 bits (981), Expect = 5.5e-106
Identity = 206/517 (39.85%), Postives = 306/517 (59.19%), Query Frame = 0

Query: 19  LFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAKEPTHITLAKMADAYGPMF 78
           LF++ +  + +     + +    P   GAWP++GHLH      PTH+T   MAD YGP+F
Sbjct: 12  LFIFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGAMADVYGPVF 71

Query: 79  TLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLGYDYAMFGLSPYGPHWRQA 138
             + G  K ++++S E+A+E +T +D++   RP+L ASKLLGY+ +    SPYG +WR+ 
Sbjct: 72  MAKLGSIKVMIINSKEVAKEIYTVHDKLL-ERPELTASKLLGYNDSFLTFSPYGLYWREI 131

Query: 139 RRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVEN---EEKAVVEMKTWFGDVTLN 198
           R++A  EL +   ++     R  E     + LY    +    +E  +V+MK  F D+T N
Sbjct: 132 RKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKRGKPKEGVLVDMKQEFIDLTAN 191

Query: 199 TIFRMVVGKRFSTAFDDNNGSE-----ECKKALRDFFGLFGIFIPSDAFQFLSWFDLGGH 258
               MV GKR+   F +N   E      C K +R+F   F +F+ SD    L + D    
Sbjct: 192 ISLMMVSGKRY---FGENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVLGFLD-WKT 251

Query: 259 EKAMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKNEAESFSYDAE 318
           ++ MK TA+ LD   + +++EH+ ++ S+ G S   E D++D++I ++  +      D  
Sbjct: 252 KRGMKRTAKGLDKVAEGWIEEHKNKR-SDHGRS---ENDYLDILIKILGQDKIPGLSDTH 311

Query: 319 TVIKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRERQVNESDVKN 378
           T IKA CLN++L G +T  V + WA+SLLLNN   L+KAQ ELD ++G+ER V E D+K+
Sbjct: 312 TKIKALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIGKERVVEELDIKD 371

Query: 379 LLYLRAIVKETLRLYPPAPLLAPHESIE--DCTAVGYYIPNGTRLIVNVKKLQRDPFVWE 438
           L+YL+AIVKET RLYPP PL+A    +E  D      ++P GT+L+V+  K+ RDP VW 
Sbjct: 372 LVYLQAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIHRDPNVWS 431

Query: 439 DPCEFRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLANLLHGFEIG 498
           +P +F PERFLTS    DV GQS +F PFG GRR CP I   ++++H  L   LH F++ 
Sbjct: 432 NPEQFEPERFLTSNRELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHSFDLA 491

Query: 499 RPSEELLDMEESVGLTSVRKTPLEIVLTPRLPAQVYE 526
           RPS + +DM ES GL + + TPLE+ + PRL   +YE
Sbjct: 492 RPSSQDVDMTESNGLVNHKATPLEVNIIPRLHKSLYE 519

BLAST of MC11g_new0034 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 363.6 bits (932), Expect = 2.6e-100
Identity = 201/512 (39.26%), Postives = 306/512 (59.77%), Query Frame = 0

Query: 14  VFALLLFLYALFTMSRKSSADRRRLPAPPEAGGAWPVIGHLHQLSAKEPTHITLAKMADA 73
           +F+L L ++    + ++ S         PE  GA P+ GHLH L  K+     LA M+  
Sbjct: 12  LFSLALVIFGYIFLRKQLSRCEVDSSTIPEPLGALPLFGHLHLLRGKKLLCKKLAAMSQK 71

Query: 74  YGPMFTLRFGMKKALVVSSWEIAREFFTTNDRVFASRPKLVASKLLGYDYAMFGLSPYGP 133
           +GP+F+L+ G  + +V S  +  ++ FTTND   A+RP +   + +GY+ A   L+PYG 
Sbjct: 72  HGPIFSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNASLTLAPYGD 131

Query: 134 HWRQARRVATLELLTNHRLERLQHVRILEVQNWIKELYKLCVENEEKAVVEMKTWFGDVT 193
           +WR+ R++ T+ L +NH +E L H+R  EV   IK LYK    N   ++V++   F  +T
Sbjct: 132 YWRELRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLYK---GNGGTSIVKIDMLFEFLT 191

Query: 194 LNTIFRMVVGKRFSTAFDDNNGSE-ECKKALRDFFGLFGIFIPSDAFQFLSWFDLGGHEK 253
            N I R +VGKR    F + N  E   K+AL+    L  I +  D   +L W D   + +
Sbjct: 192 FNIILRKMVGKRI--GFGEVNSDEWRYKEALKHCEYLAVIPMIGDVIPWLGWLDFAKNSQ 251

Query: 254 AMKETAELLDDTFDKFLKEHQQQQASNFGESEMEEQDFMDVMISVVKNEAESFSYDAETV 313
            MK   + LD    K+L EH ++++ N    + +E+  MD+++ ++  +     +  + +
Sbjct: 252 -MKRLFKELDSVNTKWLHEHLKKRSRN---EKDQERTIMDLLLDILPEDIVISGHVRDVI 311

Query: 314 IKATCLNMILGGFDTTTVTMTWAISLLLNNQEALKKAQLELDEQVGRERQVNESDVKNLL 373
           +KAT L + L G D+T++T+TWA+SLLLNN  AL+ AQ E+D  VG+ R + ESD++NL 
Sbjct: 312 VKATILALTLTGSDSTSITLTWAVSLLLNNPAALEAAQEEIDNSVGKGRWIEESDIQNLK 371

Query: 374 YLRAIVKETLRLYPPAPLLAPHESIEDCTAVGYYIPNGTRLIVNVKKLQRDPFVWEDPCE 433
           YL+AIVKET RLYPPAPL    E+ EDC   GY +  GTRL+VN+ KL RDP +W DP  
Sbjct: 372 YLQAIVKETHRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIWKLHRDPKIWPDPKT 431

Query: 434 FRPERFLTSQNNFDVRGQSPQFIPFGNGRRMCPGISFALQVIHLTLANLLHGFEIGRPSE 493
           F+PERF+  ++  +    + ++IPFG+GRR CPG++  L+V+H  LA LL GFE+ + S+
Sbjct: 432 FKPERFMEDKSQCE--KSNFEYIPFGSGRRSCPGVNLGLRVVHFVLARLLQGFELHKVSD 491

Query: 494 ELLDMEESVGLTSVRKTPLEIVLTPRLPAQVY 525
           E LDM E  GL   +  P+E+V+ PRL  ++Y
Sbjct: 492 EPLDMAEGPGLALPKINPVEVVVMPRLDPKLY 512