MC11g_new0029 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g_new0029
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	EF hand calcium-binding protein family
Location	MC11: 1374743 .. 1375629 (+)
RNA-Seq Expression	MC11g_new0029
Synteny	MC11g_new0029

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAGACCTTTTTACTCCAATTTTCATATGCTTTCTATAGTTATATATGTATATCACCACTCATACTTGTTCCTAAGAACCAAAAAAAAAAAAAAACAATGGCCATTCCAATTCCAACTGTTTCCATTGCCTTCAAATGGTTTTCCAAATCCAATTTCAAGCTCAACTTCCCTCGCCTTCTCTTCAACCCCAAACCCACCTCCTCTAATCCTCCTCCACCACCCCTCAAGGCGGCGCCACCGCTCGCCGAAGTCGCCTTTCACCACCTCGACGCTGATGGAGATGGCAAAATCTCATGCGACGAACTCCGATCCTACTTTGCATCCATCGGAGAGTGTATAACACGCGACGATGTTCGTAGAGTGATTCGAGACTTCGATGGCAACGGCGACGAGCTACTGGAGCTTCAAGACTTTGCTCGCCTAATGGAGGTCCAGGAGGAGGAGGAGGGAGAGGATTTGAGAAGGGCTTTTGAAATGTTTGAAGTTGAAAAGGGGTGCGGGTTCATCGGACCGGAGGGATTACAGAAGATGTTTCGACGGTTGGGGTATGTGAAATCGAGCGACGAGTGCATGGCAATGATCGACGTGTTTGACATTAATGGTGACGGTGTTATCGATTATCGTGAATTTCTCAGAATGATGGCAGGGCCTAATTTAACATGTGAATAAATCTCCTTTGATTACTGTCGTGTATCTGTCTTTTGTTTTTGTCAATGTTAATGAAAAGTACGCATATATATGTAATATACTTGAGTGAGGTGCTTGGATTTGGCGTTGTTGTCTCTTAATTATGTCGGCAGACTGTAAATTCTATAAGCTTTTTCATTTTTCCTGTTTTGATATTTTGAACTTTGTAATATTGAAACTGAAAATAAGGGAACAAT

mRNA sequence

ATGGCCATTCCAATTCCAACTGTTTCCATTGCCTTCAAATGGTTTTCCAAATCCAATTTCAAGCTCAACTTCCCTCGCCTTCTCTTCAACCCCAAACCCACCTCCTCTAATCCTCCTCCACCACCCCTCAAGGCGGCGCCACCGCTCGCCGAAGTCGCCTTTCACCACCTCGACGCTGATGGAGATGGCAAAATCTCATGCGACGAACTCCGATCCTACTTTGCATCCATCGGAGAGTGTATAACACGCGACGATGTTCGTAGAGTGATTCGAGACTTCGATGGCAACGGCGACGAGCTACTGGAGCTTCAAGACTTTGCTCGCCTAATGGAGGTCCAGGAGGAGGAGGAGGGAGAGGATTTGAGAAGGGCTTTTGAAATGTTTGAAGTTGAAAAGGGGTGCGGGTTCATCGGACCGGAGGGATTACAGAAGATGTTTCGACGGTTGGGGTATGTGAAATCGAGCGACGAGTGCATGGCAATGATCGACGTGTTTGACATTAATGGTGACGGTGTTATCGATTATCGTGAATTTCTCAGAATGATGGCAGGGCCTAATTTAACATGTGAATAA

Coding sequence (CDS)

ATGGCCATTCCAATTCCAACTGTTTCCATTGCCTTCAAATGGTTTTCCAAATCCAATTTCAAGCTCAACTTCCCTCGCCTTCTCTTCAACCCCAAACCCACCTCCTCTAATCCTCCTCCACCACCCCTCAAGGCGGCGCCACCGCTCGCCGAAGTCGCCTTTCACCACCTCGACGCTGATGGAGATGGCAAAATCTCATGCGACGAACTCCGATCCTACTTTGCATCCATCGGAGAGTGTATAACACGCGACGATGTTCGTAGAGTGATTCGAGACTTCGATGGCAACGGCGACGAGCTACTGGAGCTTCAAGACTTTGCTCGCCTAATGGAGGTCCAGGAGGAGGAGGAGGGAGAGGATTTGAGAAGGGCTTTTGAAATGTTTGAAGTTGAAAAGGGGTGCGGGTTCATCGGACCGGAGGGATTACAGAAGATGTTTCGACGGTTGGGGTATGTGAAATCGAGCGACGAGTGCATGGCAATGATCGACGTGTTTGACATTAATGGTGACGGTGTTATCGATTATCGTGAATTTCTCAGAATGATGGCAGGGCCTAATTTAACATGTGAATAA

Protein sequence

MAIPIPTVSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPLKAAPPLAEVAFHHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQEEEEGEDLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMMAGPNLTCE

Homology

[Close]

BLAST of MC11g_new0029 vs. ExPASy Swiss-Prot
Match: Q8L3R2 (Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 PE=2 SV=2)

HSP 1 Score: 129.8 bits (325), Expect = 3.3e-29
Identity = 80/194 (41.24%), Postives = 110/194 (56.70%), Query Frame = 0


Query: 9   SIAFKWFSKSNFKLN--FPRLLFNPKPTSSNPPPPPL------------KAAPPLAEVAF 68
           S +FKWFS    KLN  F     +PK  SS+    P             +A+       F
Sbjct: 10  SNSFKWFSTKTLKLNLSFQNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQASKEELRQVF 69

Query: 69  HHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQE 128
            H D+DGDGKIS  ELR YF S+GE I+ +  +  I + D + D  L  +DF  LM  ++
Sbjct: 70  SHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLMTRRD 129

Query: 129 -----EEEGE-DLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDIN 183
                E +G+ +L+ AFEMFEVEKG G I P+GLQKM  +LG  ++  EC AMI  +DI+
Sbjct: 130 LYGDGEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFYDID 189

[Close]

BLAST of MC11g_new0029 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.8e-20
Identity = 48/133 (36.09%), Postives = 88/133 (66.17%), Query Frame = 0


Query: 51  EVAFHHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLM 110
           E  F ++DA+ DG+IS +EL+  F ++GE ++ ++    +R  D +GD +L+ ++F++L+
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLI 104

Query: 111 EVQEEEEGE-DLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDING 170
           +V +EEE + +L+ AF ++ + +G   I P  L+ M ++LG  +++D+C  MI  FD+N 
Sbjct: 105 KVDDEEEKKMELKGAFRLY-IAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 171 DGVIDYREFLRMM 183
           DGV+ + EF  MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of MC11g_new0029 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 3.6e-20
Identity = 62/144 (43.06%), Postives = 88/144 (61.11%), Query Frame = 0


Query: 45  AAPPLAEVAFHHLDADGDGKISCDELRSYF-ASIGECITRDDVRRVIRDFDGNGDELLEL 104
           A+P  +   F   D DGDG+IS  ELR     ++GE ++ ++  +++   D +GD LL  
Sbjct: 7   ASPCESSALFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCE 66

Query: 105 QDFARLM---EVQEEEE--GEDLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDEC 164
            +F RL+   EV+EE+E  G  LR AF M+E+E G G I P  L++M RRLG  +  D+C
Sbjct: 67  AEFVRLVQAAEVEEEDERRGTGLREAFGMYEME-GEGCITPTSLRRMLRRLGSDQDIDDC 126

Query: 165 MAMIDVFDINGDGVIDYREFLRMM 183
            AMI  FD+NGDGV+ + EF  MM
Sbjct: 127 RAMICRFDLNGDGVLSFDEFKIMM 149

[Close]

BLAST of MC11g_new0029 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.1e-19
Identity = 48/135 (35.56%), Postives = 85/135 (62.96%), Query Frame = 0


Query: 54  FHHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLME-- 113
           F   D +GDGKI+ +EL+ +F S+G  +  +++  +I   D NGD  +++ +F  L +  
Sbjct: 10  FQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSLYQEM 69

Query: 114 VQEEEEGEDLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYV--KSSDECMAMIDVFDING 173
           V+E+EE ED+R AF +F+ + G GFI  E L+ +   +G    ++ ++C  MI   D++G
Sbjct: 70  VEEKEEEEDMREAFRVFD-QNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDVDG 129

Query: 174 DGVIDYREFLRMMAG 185
           DG+++++EF +MM G
Sbjct: 130 DGMVNFKEFKQMMRG 143

[Close]

BLAST of MC11g_new0029 vs. ExPASy Swiss-Prot
Match: Q6L4D4 (Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 GN=CML15 PE=2 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 3.1e-19
Identity = 60/164 (36.59%), Postives = 92/164 (56.10%), Query Frame = 0


Query: 29  FNPKPT------SSNPPPPPLKAAPPLAEVAFHHLDADGDGKISCDELRSYFASIGECIT 88
           ++P+P+      ++ P PP   AA    E  F   DA+GDG+IS  EL + F S+G  +T
Sbjct: 32  WSPRPSDLAAAAAAKPRPP---AAEDETERVFRKFDANGDGRISRAELAALFRSVGHAVT 91

Query: 89  RDDVRRVIRDFDGNGDELLELQDFARLMEVQEEEEG---EDLRRAFEMFEVEKGCGFIGP 148
            D+V R++++ D +GD  + L +FA +      +     EDLR AF +F+ + G G I P
Sbjct: 92  DDEVARMMQEADSDGDGYISLGEFAAISAPPPGDAAAAEEDLRHAFGVFDAD-GNGVITP 151

Query: 149 EGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMMA 184
             L ++ R +G   +  +C  MID  D NGDG+I++ EF  MMA
Sbjct: 152 AELARVLRGIGEAATVAQCRRMIDGVDRNGDGLINFEEFKLMMA 191

[Close]

BLAST of MC11g_new0029 vs. NCBI nr
Match: XP_022139654.1 (probable calcium-binding protein CML41 [Momordica charantia])

HSP 1 Score: 389 bits (1000), Expect = 1.12e-136
Identity = 190/190 (100.00%), Postives = 190/190 (100.00%), Query Frame = 0


Query: 1   MAIPIPTVSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPLKAAPPLAEVAFHHLDAD 60
           MAIPIPTVSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPLKAAPPLAEVAFHHLDAD
Sbjct: 1   MAIPIPTVSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPLKAAPPLAEVAFHHLDAD 60

Query: 61  GDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQEEEEGED 120
           GDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQEEEEGED
Sbjct: 61  GDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQEEEEGED 120

Query: 121 LRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLR 180
           LRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLR
Sbjct: 121 LRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLR 180

Query: 181 MMAGPNLTCE 190
           MMAGPNLTCE
Sbjct: 181 MMAGPNLTCE 190

[Close]

BLAST of MC11g_new0029 vs. NCBI nr
Match: XP_022974919.1 (probable calcium-binding protein CML41 [Cucurbita maxima])

HSP 1 Score: 266 bits (679), Expect = 1.22e-87
Identity = 134/179 (74.86%), Postives = 148/179 (82.68%), Query Frame = 0


Query: 8   VSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPL---KAAPPLAEVAFHHLDADGDGK 67
           +S  FKWFS +NFKLNFPRL F  KPT SNPPPPPL     APPLAE AF HLDADGDGK
Sbjct: 20  ISTPFKWFSNTNFKLNFPRLRFKHKPTPSNPPPPPLLLLTPAPPLAEAAFRHLDADGDGK 79

Query: 68  ISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQEEEEGEDLRRA 127
           IS DEL+SYFAS+GE +TRDD R +IR+FDG+GD LLEL DF RL++  EEEE EDL+ A
Sbjct: 80  ISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEEEEKEDLKMA 139

Query: 128 FEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMMA 183
           FEMFE EKGCGFIGP GLQKMF RLGYVKS +ECMAMI VFD++GDGVIDYREFLRMM 
Sbjct: 140 FEMFEGEKGCGFIGPPGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFLRMMT 198

[Close]

BLAST of MC11g_new0029 vs. NCBI nr
Match: KAG6591338.1 (putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 263 bits (673), Expect = 6.19e-87
Identity = 133/179 (74.30%), Postives = 147/179 (82.12%), Query Frame = 0


Query: 8   VSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPL---KAAPPLAEVAFHHLDADGDGK 67
           +S  FKWFS +N KLN PRL F  KPT SNPPPPPL     APPLAE AF HLDADGDGK
Sbjct: 6   ISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDADGDGK 65

Query: 68  ISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQEEEEGEDLRRA 127
           IS DEL+SYFAS+GE +TRDD R +IR+FDG+GD LLEL DF RL++  EEEE EDL+RA
Sbjct: 66  ISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEEEEKEDLKRA 125

Query: 128 FEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMMA 183
           FEMFE EKGCGFIGP GLQKMF RLGYVKS +ECMAMI VFD++GDGVIDYREFLRMM 
Sbjct: 126 FEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFLRMMT 184

[Close]

BLAST of MC11g_new0029 vs. NCBI nr
Match: XP_023535419.1 (calmodulin-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 263 bits (673), Expect = 2.16e-86
Identity = 134/179 (74.86%), Postives = 147/179 (82.12%), Query Frame = 0


Query: 8   VSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPL---KAAPPLAEVAFHHLDADGDGK 67
           VS  FKWFS +NF LNFPRL F  KPT SNPPPPPL     APPLAE AF HLDADGDGK
Sbjct: 43  VSTPFKWFSNTNFNLNFPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDADGDGK 102

Query: 68  ISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQEEEEGEDLRRA 127
           IS DEL+SYFAS+GE +TRDD R +IR+FD +GD LLEL DF RL++  EEEE EDL+RA
Sbjct: 103 ISGDELQSYFASVGEYMTRDDARSLIREFDWDGDLLLELGDFERLVKGGEEEEKEDLKRA 162

Query: 128 FEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMMA 183
           FEMFE EKGCGFIGP GLQKMF RLGYVKS +ECMAMI VFD++GDGVIDYREFLRMM 
Sbjct: 163 FEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFLRMMT 221

[Close]

BLAST of MC11g_new0029 vs. NCBI nr
Match: KAG7024217.1 (putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 262 bits (669), Expect = 2.52e-86
Identity = 132/179 (73.74%), Postives = 147/179 (82.12%), Query Frame = 0


Query: 8   VSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPL---KAAPPLAEVAFHHLDADGDGK 67
           +S  FKWFS +N KLN PRL F  KPT SNPPPPPL     APPLAE AF HLDADGDGK
Sbjct: 6   ISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDADGDGK 65

Query: 68  ISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQEEEEGEDLRRA 127
           IS DEL+SYFAS+GE +TRD+ R +IR+FDG+GD LLEL DF RL++  EEEE EDL+RA
Sbjct: 66  ISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVKGGEEEEKEDLKRA 125

Query: 128 FEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMMA 183
           FEMFE EKGCGFIGP GLQKMF RLGYVKS +ECMAMI VFD++GDGVIDYREFLRMM 
Sbjct: 126 FEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFLRMMT 184

[Close]

BLAST of MC11g_new0029 vs. ExPASy TrEMBL
Match: A0A6J1CDM6 (probable calcium-binding protein CML41 OS=Momordica charantia OX=3673 GN=LOC111010501 PE=4 SV=1)

HSP 1 Score: 389 bits (1000), Expect = 5.42e-137
Identity = 190/190 (100.00%), Postives = 190/190 (100.00%), Query Frame = 0


Query: 1   MAIPIPTVSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPLKAAPPLAEVAFHHLDAD 60
           MAIPIPTVSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPLKAAPPLAEVAFHHLDAD
Sbjct: 1   MAIPIPTVSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPLKAAPPLAEVAFHHLDAD 60

Query: 61  GDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQEEEEGED 120
           GDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQEEEEGED
Sbjct: 61  GDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQEEEEGED 120

Query: 121 LRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLR 180
           LRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLR
Sbjct: 121 LRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLR 180

Query: 181 MMAGPNLTCE 190
           MMAGPNLTCE
Sbjct: 181 MMAGPNLTCE 190

[Close]

BLAST of MC11g_new0029 vs. ExPASy TrEMBL
Match: A0A6J1IIY2 (probable calcium-binding protein CML41 OS=Cucurbita maxima OX=3661 GN=LOC111473738 PE=4 SV=1)

HSP 1 Score: 266 bits (679), Expect = 5.89e-88
Identity = 134/179 (74.86%), Postives = 148/179 (82.68%), Query Frame = 0


Query: 8   VSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPL---KAAPPLAEVAFHHLDADGDGK 67
           +S  FKWFS +NFKLNFPRL F  KPT SNPPPPPL     APPLAE AF HLDADGDGK
Sbjct: 20  ISTPFKWFSNTNFKLNFPRLRFKHKPTPSNPPPPPLLLLTPAPPLAEAAFRHLDADGDGK 79

Query: 68  ISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQEEEEGEDLRRA 127
           IS DEL+SYFAS+GE +TRDD R +IR+FDG+GD LLEL DF RL++  EEEE EDL+ A
Sbjct: 80  ISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEEEEKEDLKMA 139

Query: 128 FEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMMA 183
           FEMFE EKGCGFIGP GLQKMF RLGYVKS +ECMAMI VFD++GDGVIDYREFLRMM 
Sbjct: 140 FEMFEGEKGCGFIGPPGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFLRMMT 198

[Close]

BLAST of MC11g_new0029 vs. ExPASy TrEMBL
Match: A0A6J1F7Y7 (probable calcium-binding protein CML41 OS=Cucurbita moschata OX=3662 GN=LOC111442970 PE=4 SV=1)

HSP 1 Score: 261 bits (666), Expect = 3.37e-86
Identity = 131/178 (73.60%), Postives = 147/178 (82.58%), Query Frame = 0


Query: 8   VSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPL--KAAPPLAEVAFHHLDADGDGKI 67
           +S  FKWFS +N KLN PRL F  KPT SNPPPPPL    APPLAE AF HLDADGDGKI
Sbjct: 6   ISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLTPAPPLAEAAFRHLDADGDGKI 65

Query: 68  SCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQEEEEGEDLRRAF 127
           S DEL+SYFAS+GE +TRDD R +IR+FDG+GD LLEL DF RL++  E+EE EDL+RAF
Sbjct: 66  SGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEQEEKEDLKRAF 125

Query: 128 EMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMMA 183
           EMFE EKGCGFIGP GLQKMF RLGYVKS +ECMAMI VFD++G+GVIDYREFLRMM 
Sbjct: 126 EMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGNGVIDYREFLRMMT 183

[Close]

BLAST of MC11g_new0029 vs. ExPASy TrEMBL
Match: A0A5A7UHX6 (Putative calcium-binding protein CML41 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27333G00010 PE=4 SV=1)

HSP 1 Score: 243 bits (619), Expect = 3.79e-79
Identity = 125/179 (69.83%), Postives = 141/179 (78.77%), Query Frame = 0


Query: 5   IPTVSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPLKAAPPLAEVAFHHLDADGDGK 64
           I T++I FKWF+K+NFKLNFP L    KP SS PP PP     PLAEVAF HLD DGDGK
Sbjct: 3   ISTIAIPFKWFTKTNFKLNFPLLHSKHKPISSKPPTPP-----PLAEVAFRHLDIDGDGK 62

Query: 65  ISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQEEEEGEDLRRA 124
           IS DELRSYFASIGE +T D  + VI DFD +GDELLEL DF RL++ +EEE+ EDL+RA
Sbjct: 63  ISGDELRSYFASIGEYMTWDGAQSVIGDFDKDGDELLELGDFERLVKGEEEEQEEDLKRA 122

Query: 125 FEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMMA 183
           FEMFE EKGCGFIG  GLQKMF RLGYVKS ++CM MI VFD++GDGVIDY EFLRMM 
Sbjct: 123 FEMFEGEKGCGFIGATGLQKMFGRLGYVKSKEDCMTMIKVFDVDGDGVIDYHEFLRMMT 176

[Close]

BLAST of MC11g_new0029 vs. ExPASy TrEMBL
Match: A0A1S3CT00 (probable calcium-binding protein CML41 OS=Cucumis melo OX=3656 GN=LOC103503982 PE=4 SV=1)

HSP 1 Score: 243 bits (619), Expect = 3.79e-79
Identity = 125/179 (69.83%), Postives = 141/179 (78.77%), Query Frame = 0


Query: 5   IPTVSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPLKAAPPLAEVAFHHLDADGDGK 64
           I T++I FKWF+K+NFKLNFP L    KP SS PP PP     PLAEVAF HLD DGDGK
Sbjct: 3   ISTIAIPFKWFTKTNFKLNFPLLHSKHKPISSKPPTPP-----PLAEVAFRHLDIDGDGK 62

Query: 65  ISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQEEEEGEDLRRA 124
           IS DELRSYFASIGE +T D  + VI DFD +GDELLEL DF RL++ +EEE+ EDL+RA
Sbjct: 63  ISGDELRSYFASIGEYMTWDGAQSVIGDFDKDGDELLELGDFERLVKGEEEEQEEDLKRA 122

Query: 125 FEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMMA 183
           FEMFE EKGCGFIG  GLQKMF RLGYVKS ++CM MI VFD++GDGVIDY EFLRMM 
Sbjct: 123 FEMFEGEKGCGFIGATGLQKMFGRLGYVKSKEDCMTMIKVFDVDGDGVIDYHEFLRMMT 176

[Close]

BLAST of MC11g_new0029 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 129.8 bits (325), Expect = 2.3e-30
Identity = 80/194 (41.24%), Postives = 110/194 (56.70%), Query Frame = 0


Query: 9   SIAFKWFSKSNFKLN--FPRLLFNPKPTSSNPPPPPL------------KAAPPLAEVAF 68
           S +FKWFS    KLN  F     +PK  SS+    P             +A+       F
Sbjct: 10  SNSFKWFSTKTLKLNLSFQNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQASKEELRQVF 69

Query: 69  HHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQE 128
            H D+DGDGKIS  ELR YF S+GE I+ +  +  I + D + D  L  +DF  LM  ++
Sbjct: 70  SHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLMTRRD 129

Query: 129 -----EEEGE-DLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDIN 183
                E +G+ +L+ AFEMFEVEKG G I P+GLQKM  +LG  ++  EC AMI  +DI+
Sbjct: 130 LYGDGEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFYDID 189

[Close]

BLAST of MC11g_new0029 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 100.1 bits (248), Expect = 2.0e-21
Identity = 48/133 (36.09%), Postives = 88/133 (66.17%), Query Frame = 0


Query: 51  EVAFHHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLM 110
           E  F ++DA+ DG+IS +EL+  F ++GE ++ ++    +R  D +GD +L+ ++F++L+
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLI 104

Query: 111 EVQEEEEGE-DLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDING 170
           +V +EEE + +L+ AF ++ + +G   I P  L+ M ++LG  +++D+C  MI  FD+N 
Sbjct: 105 KVDDEEEKKMELKGAFRLY-IAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 171 DGVIDYREFLRMM 183
           DGV+ + EF  MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of MC11g_new0029 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 100.1 bits (248), Expect = 2.0e-21
Identity = 48/133 (36.09%), Postives = 88/133 (66.17%), Query Frame = 0


Query: 51  EVAFHHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLM 110
           E  F ++DA+ DG+IS +EL+  F ++GE ++ ++    +R  D +GD +L+ ++F++L+
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLI 104

Query: 111 EVQEEEEGE-DLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDING 170
           +V +EEE + +L+ AF ++ + +G   I P  L+ M ++LG  +++D+C  MI  FD+N 
Sbjct: 105 KVDDEEEKKMELKGAFRLY-IAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 171 DGVIDYREFLRMM 183
           DGV+ + EF  MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of MC11g_new0029 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 98.2 bits (243), Expect = 7.5e-21
Identity = 48/135 (35.56%), Postives = 85/135 (62.96%), Query Frame = 0


Query: 54  FHHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLME-- 113
           F   D +GDGKI+ +EL+ +F S+G  +  +++  +I   D NGD  +++ +F  L +  
Sbjct: 10  FQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSLYQEM 69

Query: 114 VQEEEEGEDLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYV--KSSDECMAMIDVFDING 173
           V+E+EE ED+R AF +F+ + G GFI  E L+ +   +G    ++ ++C  MI   D++G
Sbjct: 70  VEEKEEEEDMREAFRVFD-QNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDVDG 129

Query: 174 DGVIDYREFLRMMAG 185
           DG+++++EF +MM G
Sbjct: 130 DGMVNFKEFKQMMRG 143

[Close]

BLAST of MC11g_new0029 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 93.6 bits (231), Expect = 1.8e-19
Identity = 48/136 (35.29%), Postives = 81/136 (59.56%), Query Frame = 0


Query: 51  EVAFHHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLM 110
           E  F ++DA+ DG+IS +EL+  F ++GE ++ ++    ++  D +GD +L++ +FA L+
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 111 ----EVQEEEEGEDLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFD 170
               E  EEE+   +  AF M+ +  G   I P  L+ M  +LG  +++D+C  MI  FD
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMY-IADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFD 143

Query: 171 INGDGVIDYREFLRMM 183
           +N DGV+ + EF  MM
Sbjct: 144 LNADGVLSFDEFALMM 158

The following BLAST results are available for this feature:

BLAST of MC11g_new0029 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L3R2	3.3e-29	41.24	Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 ...	[more]
Q9SRE6	2.8e-20	36.09	Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1	[more]
Q8RYK0	3.6e-20	43.06	Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SU00	1.1e-19	35.56	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]
Q6L4D4	3.1e-19	36.59	Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of MC11g_new0029 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139654.1	1.12e-136	100.00	probable calcium-binding protein CML41 [Momordica charantia]	[more]
XP_022974919.1	1.22e-87	74.86	probable calcium-binding protein CML41 [Cucurbita maxima]	[more]
KAG6591338.1	6.19e-87	74.30	putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_023535419.1	2.16e-86	74.86	calmodulin-like protein 2 [Cucurbita pepo subsp. pepo]	[more]
KAG7024217.1	2.52e-86	73.74	putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. a...	[more]

BLAST of MC11g_new0029 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CDM6	5.42e-137	100.00	probable calcium-binding protein CML41 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1IIY2	5.89e-88	74.86	probable calcium-binding protein CML41 OS=Cucurbita maxima OX=3661 GN=LOC1114737...	[more]
A0A6J1F7Y7	3.37e-86	73.60	probable calcium-binding protein CML41 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A5A7UHX6	3.79e-79	69.83	Putative calcium-binding protein CML41 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CT00	3.79e-79	69.83	probable calcium-binding protein CML41 OS=Cucumis melo OX=3656 GN=LOC103503982 P...	[more]

BLAST of MC11g_new0029 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G50770.1	2.3e-30	41.24	calmodulin-like 41	[more]
AT1G76650.1	2.0e-21	36.09	calmodulin-like 38	[more]
AT1G76650.3	2.0e-21	36.09	calmodulin-like 38	[more]
AT4G12860.1	7.5e-21	35.56	EF hand calcium-binding protein family	[more]
AT1G76640.1	1.8e-19	35.29	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 49..77 e-value: 0.067 score: 22.3 coord: 157..185 e-value: 3.8E-5 score: 33.1 coord: 85..113 e-value: 1.3 score: 16.7
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 53..110 e-value: 8.9E-12 score: 45.3
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 135..182 e-value: 2.5E-9 score: 36.8
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 45..80 score: 12.114401
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 153..188 score: 12.42125
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 81..116 score: 9.687501
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 120..183 e-value: 8.61756E-11 score: 53.3205
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 51..110 e-value: 4.40953E-11 score: 54.0909
None	No IPR available	GENE3D	1.10.238.10		coord: 115..184 e-value: 6.5E-17 score: 63.3
None	No IPR available	GENE3D	1.10.238.10		coord: 34..114 e-value: 1.3E-15 score: 59.9
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 37..184
None	No IPR available	PANTHER	PTHR23050:SF393	CALMODULIN	coord: 37..184
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 58..70
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 166..178
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 53..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g_new0029.1	MC11g_new0029.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0030234	enzyme regulator activity