MC11g1458 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC11g1458
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptiontranscription factor bHLH80-like
LocationMC11: 19507199 .. 19510923 (-)
RNA-Seq ExpressionMC11g1458
SyntenyMC11g1458
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CCGAGTCTGAATCAGCGTTCATCTCCGTCTCCGGCCGTAACCAACAGATCAGATGGTTCGTATAATTCAGAAATTGGGTATTACCGTACACCGCACCCGCACTGGATCAGGCTCAGGCTCAGGCTCAGGCTCAGGGAAAAACACAAAACAAAATAAAAAAAGAGAAGAGGAACAATCTGGTCCCTTTGCTGTGACCCTTTCCAAACATAGATAGAATTCTGAATTCTTCCCCAACAAAAATAGAACCCAACCCACGCAAGTTTTTCCTTTCAGCAACCAAATAACCCAGAAAAAGAAAACCATAAAAAAGAACAGGAAAGCCATCAGAAATCTCCTGAACCAGTCAGTGGGAAGCAGATGCAATCCACAACTGGTGGCGGTACTACCAGCAGCGGCGGCGGCGACCTCGGGCCCCGTGCTAGCGGACTCGCTCGCTTCCGCTCAGCTCCGGCCACCTGGTTGGAAGCGCTTCTAGAGGACGACGAGGAAGACCCTCTGAAGCCCAATCAGTGCTTGACTCAGCTTCTCGCCGGCAACTCCCCGGCCCTCGGTTCCCCGCCGGATCAAGGCCTTTTCGACGCCAATCCCAATCCCAATCCCTCGCCCGCCTTCGCCCGCCAGAATAGTTCTCCGGCCGAGTTTCTCGGCGAGGGTTTCTACTCTGCCTATGGTGTTACTTCCGCTAAGTACGAATTGCCCTCCCCGGGTCTCGACATCTCTCCCACGTCCAAGCGCGGGAGAGAGGTGGAGGCCCAAAATTTCCACTCCAAATTTTCGTCTCAGCTGGTAATCTCTTTAAGCTCTGTTTCGCGATTCGAAATCAGGAGTAGAAATTATCTGATTTGATTTGATTTGATTTGATTTGAGAGTAGAGCAGAGTTAAGTTGAGTAGTCAAGTATGTGGGATAGTGAATAATAGTTTGATTTTGTAAGTGAAATTTTGGGCAGAAAAGGGAGGAAAGTGGGGTTTCCAGTTTGTTAGACATGGAAATGGAGAAGCTATTGGAGGACTCTGTCCCCTGCCGTGTTCGGGCCAAGCGCGGCTGTGCTACGCATCCTCGGAGCATTGCTGAGAGGGTTAGTTTCTCATCTTTCGCCTGAATCTTCTTTCAACTTCATCCAAGTAAACCCATCATGTGGATTTTAACATAGCACCAGCAGTTGCAGATTGGTTCATTGGGGACGTTTCATCCTTTGAACTTTGATGCTTGTGTCTAAAAGTTTGATTTAAAATGTATTTTGCCCATTTTCTCTGTTCTTGTTTTATCAAGCTAGTTTGCTTCCTTGTGTAACATGTTGCCACGCGTCCTTATTCAATCCGTATGTCATTTTAACACACACAACAAAAAGAGATGTCCATTAATTATTAATTTCGTTGTTTCCGTGGGTAGGTTCGCAGGACTAAAATTAGTGACAGAATAAGGAAGCTTCAGGAGGTTGTGCCCAACATGGATAAGGTAACTATACCATGATTGATCATAGGAGTACAAATGAATTTAGATCTATCTTATATGTTATCAGCTGTGTGCGCGTGCGCATATATTAAGAAATGACTATGTACTTGTCCTGCCATGTGCTTATTCTTGCCGTTCTACCATGAATTCCATTCTAAACTTCCAGTTTGACTTGAATTTAACTTTATGCATGATTATTAATGAAATCTAAGTCTAACATTAGACAATTAGTTGAGTAATGGAGACAAGATTGTGTGGTTTGAACTTAAAGGTCGATCTCTTTCAAGTTTCATCTGTATTGTTTTTAATCTATACTGTACTAATATCAAATGACATTAAACTATTAGATTATGAGTGATTTATTTATTCATACCAGTTTGTAATAGTTTCTGTTAATCAATCAGAAGAATGATTAGTGCACTTTTCATCCGGTGGGCAACAAAGCTATCTTACAAATTGTTGCACGGGTAAAGTTTCCTGTATTTTACCGAAGTTACAATTAAATGGACATGACATATATGTTATAATTTATTTCATTTGAGGTATTTTCGTGTATTGATTGTGGATTGTGCAAGGGAATGTCAGTCTATTAATATGAAAAGAAATTATAACATCTATTGAACTAGTTTTCAAGGTTGATGGAGCTCATTAATACTAAACATATGTATTTAGACCTTCAGTTACATCCTTTATGCCCAATAGTCCATGAAGATTAGGTTGACTAGGAAAGCAACTTATATCAGATGTGTAAGGGTGAGATTAGGATTATTTCTTCGGTTGAGAGAGTATTTGAACATCGATGTATGCGTTTGTCTATATACATAAATATATGTATGGATTTATGGATGTAATAAACTATCATGGGATGACCTAATGGTCAATAAAGGCCAATGTAAATAGCAAAGGGTTTAGAGGGATTGGGTTCAAATCATAGTGGCCACCTACCTAGGATTTAATATCCTACGAGTTACCTTGTCAACCAAATGTAGTAGGGTCAGGCAGTTGGCTCGTGATTAGTCGAGATGCATGCAAGCTGGCCTGTGCATAGCATAGTTGTTTGACCTTGTCGGTTGACATCCGAACAGCTCTCATTGTCTTTAACTTTTGTGTCATTCCTTTCCCGTCAATTTTTTACCTTCAGGTGGTTGCTATTTTTTTTCTTTTTTAATTTGGTGAAAAAAGACAACTTTCAAATTTATTGACTGTTTTACGCGCTACCATTCATAACTATTGGTCAAGCATGGAAAGAACTTTAATGCATTTTGGTGACTTGCCTTATCTAGATGGTTGATTTTGAGATTTGGGCTTTTGAATATTTAAATTTCTCATGGTAAGACCACTAAAATTTCCATAGTACGTTTGAAATTAAAGCTACATGGTTTTTCTTTTGAATTTTACTCCAAAGGTCTTATGTCAAAGGGAGATTAAAAAATCTTGTCCACTTATAACTAATTTAAAAGTTTATTGTTCAATATCGATTAACTTAGAACGTGAAACGATGACGTGTATATTTTGTTTGGTAAATTTATGTATTGATTTGTTGTTTTGGTCTTGCAGCAGACGAACACTGCAGATATGTTAGAAGAAGCAGTAGAGTATGTCAAATTCCTTCAAAGGCAGATTCAGGTATACAATTCAACTGAACTAGATCCTGTTCATGATTTTCTATTAGGTTATATGGAAACTGCTAGAAACTGGAGAATCATTTCTTCGGCTATTACTTCGATTTTGACCCCTAATCTGTAGTCGCATAGCTATTATAAGTTTCAATTGAAATGTGTCGAAATTTTGAGAAACTGAAGTTTCCTGAAAACCGACAGGAACTCACAGAGCACCAGCGCGGGTGCAAATGCATGGTTAAGGAATAATAGGTCGAACGAAGAACGCCACTCCGGGCTCACAGAAATCGTGGTTCCACACGTTTAGTGTAAATTAGTAGATTTCTGAGGACTTGAAAGGCAGTAAAGTAAATGAAGGTGTTTTAGCTACAAAACAATATTGTATTCTCTCTGGTCCTAAGGAAAATATCAACGTTGTAATGCATGCATTGAAATATATTGCATTCCTACGCAATAAACAGGTGTAACAAAATGCTGGAGAAATTTGCTAAATTATTGTTTTTTTTTTGGGGGGAGGTTGGGGATTTTTTAAAAAATTATTGAATAGAACATTTAGATCATTAGGTATCTGATTTAAGGTACTTGTTCTGTGTATTGTATTTTGAATTTCCACCCAATATTATCAAGAAAAAGCAATGGGATTGAAATTGCA

mRNA sequence

CCGAGTCTGAATCAGCGTTCATCTCCGTCTCCGGCCGTAACCAACAGATCAGATGGTTCGTATAATTCAGAAATTGGGTATTACCGTACACCGCACCCGCACTGGATCAGGCTCAGGCTCAGGCTCAGGCTCAGGGAAAAACACAAAACAAAATAAAAAAAGAGAAGAGGAACAATCTGGTCCCTTTGCTGTGACCCTTTCCAAACATAGATAGAATTCTGAATTCTTCCCCAACAAAAATAGAACCCAACCCACGCAAGTTTTTCCTTTCAGCAACCAAATAACCCAGAAAAAGAAAACCATAAAAAAGAACAGGAAAGCCATCAGAAATCTCCTGAACCAGTCAGTGGGAAGCAGATGCAATCCACAACTGGTGGCGGTACTACCAGCAGCGGCGGCGGCGACCTCGGGCCCCGTGCTAGCGGACTCGCTCGCTTCCGCTCAGCTCCGGCCACCTGGTTGGAAGCGCTTCTAGAGGACGACGAGGAAGACCCTCTGAAGCCCAATCAGTGCTTGACTCAGCTTCTCGCCGGCAACTCCCCGGCCCTCGGTTCCCCGCCGGATCAAGGCCTTTTCGACGCCAATCCCAATCCCAATCCCTCGCCCGCCTTCGCCCGCCAGAATAGTTCTCCGGCCGAGTTTCTCGGCGAGGGTTTCTACTCTGCCTATGGTGTTACTTCCGCTAAGTACGAATTGCCCTCCCCGGGTCTCGACATCTCTCCCACGTCCAAGCGCGGGAGAGAGGTGGAGGCCCAAAATTTCCACTCCAAATTTTCGTCTCAGCTGAAAAGGGAGGAAAGTGGGGTTTCCAGTTTGTTAGACATGGAAATGGAGAAGCTATTGGAGGACTCTGTCCCCTGCCGTGTTCGGGCCAAGCGCGGCTGTGCTACGCATCCTCGGAGCATTGCTGAGAGGGTTCGCAGGACTAAAATTAGTGACAGAATAAGGAAGCTTCAGGAGGTTGTGCCCAACATGGATAAGCAGACGAACACTGCAGATATGTTAGAAGAAGCAGTAGAGTATGTCAAATTCCTTCAAAGGCAGATTCAGGAACTCACAGAGCACCAGCGCGGGTGCAAATGCATGGTTAAGGAATAATAGGTCGAACGAAGAACGCCACTCCGGGCTCACAGAAATCGTGGTTCCACACGTTTAGTGTAAATTAGTAGATTTCTGAGGACTTGAAAGGCAGTAAAGTAAATGAAGGTGTTTTAGCTACAAAACAATATTGTATTCTCTCTGGTCCTAAGGAAAATATCAACGTTGTAATGCATGCATTGAAATATATTGCATTCCTACGCAATAAACAGGTGTAACAAAATGCTGGAGAAATTTGCTAAATTATTGTTTTTTTTTTGGGGGGAGGTTGGGGATTTTTTAAAAAATTATTGAATAGAACATTTAGATCATTAGGTATCTGATTTAAGGTACTTGTTCTGTGTATTGTATTTTGAATTTCCACCCAATATTATCAAGAAAAAGCAATGGGATTGAAATTGCA

Coding sequence (CDS)

ATGCAATCCACAACTGGTGGCGGTACTACCAGCAGCGGCGGCGGCGACCTCGGGCCCCGTGCTAGCGGACTCGCTCGCTTCCGCTCAGCTCCGGCCACCTGGTTGGAAGCGCTTCTAGAGGACGACGAGGAAGACCCTCTGAAGCCCAATCAGTGCTTGACTCAGCTTCTCGCCGGCAACTCCCCGGCCCTCGGTTCCCCGCCGGATCAAGGCCTTTTCGACGCCAATCCCAATCCCAATCCCTCGCCCGCCTTCGCCCGCCAGAATAGTTCTCCGGCCGAGTTTCTCGGCGAGGGTTTCTACTCTGCCTATGGTGTTACTTCCGCTAAGTACGAATTGCCCTCCCCGGGTCTCGACATCTCTCCCACGTCCAAGCGCGGGAGAGAGGTGGAGGCCCAAAATTTCCACTCCAAATTTTCGTCTCAGCTGAAAAGGGAGGAAAGTGGGGTTTCCAGTTTGTTAGACATGGAAATGGAGAAGCTATTGGAGGACTCTGTCCCCTGCCGTGTTCGGGCCAAGCGCGGCTGTGCTACGCATCCTCGGAGCATTGCTGAGAGGGTTCGCAGGACTAAAATTAGTGACAGAATAAGGAAGCTTCAGGAGGTTGTGCCCAACATGGATAAGCAGACGAACACTGCAGATATGTTAGAAGAAGCAGTAGAGTATGTCAAATTCCTTCAAAGGCAGATTCAGGAACTCACAGAGCACCAGCGCGGGTGCAAATGCATGGTTAAGGAATAA

Protein sequence

MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGNSPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFLGEGFYSAYGVTSAKYELPSPGLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDMEMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQRQIQELTEHQRGCKCMVKE
Homology
BLAST of MC11g1458 vs. ExPASy Swiss-Prot
Match: Q9C8P8 (Transcription factor bHLH80 OS=Arabidopsis thaliana OX=3702 GN=BHLH80 PE=2 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.2e-68
Identity = 158/268 (58.96%), Postives = 189/268 (70.52%), Query Frame = 0

Query: 1   MQSTTGGGTTSSGGGDLGPRA--SGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLA 60
           MQST   G +S GGG  G     SGL+R RSAPATW+E LLE+DEE+ LKPN CLT+LL 
Sbjct: 1   MQSTHISGGSSGGGGGGGGEVSRSGLSRIRSAPATWIETLLEEDEEEGLKPNLCLTELLT 60

Query: 61  GNSPALG------------SPPDQGLFDANPNPNPSPAFARQNSSPAEFLG------EGF 120
           GN+ + G            S  +QGL+    N +    F RQNSSPA+FL       +G+
Sbjct: 61  GNNNSGGVITSRDDSFEFLSSVEQGLY----NHHQGGGFHRQNSSPADFLSGSGSGTDGY 120

Query: 121 YSAYGVTSAKYELPSPGLDISPTSKRGREVEAQNFHSKFSSQLKREE--SGVSSLLDMEM 180
           +S +G+  A Y+  S  +DISPT KR R++E Q     FSSQLK E+   G+S ++DM M
Sbjct: 121 FSNFGI-PANYDYLSTNVDISPT-KRSRDMETQ-----FSSQLKEEQMSGGISGMMDMNM 180

Query: 181 EKLLEDSVPCRVRAKRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEE 240
           +K+ EDSVPCRVRAKRGCATHPRSIAERVRRT+ISDRIR+LQE+VPNMDKQTNTADMLEE
Sbjct: 181 DKIFEDSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRRLQELVPNMDKQTNTADMLEE 240

Query: 241 AVEYVKFLQRQIQELTEHQRGCKCMVKE 247
           AVEYVK LQ QIQELTE Q+ CKC  KE
Sbjct: 241 AVEYVKALQSQIQELTEQQKRCKCKPKE 257

BLAST of MC11g1458 vs. ExPASy Swiss-Prot
Match: Q9M0R0 (Transcription factor bHLH81 OS=Arabidopsis thaliana OX=3702 GN=BHLH81 PE=2 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 1.0e-67
Identity = 158/261 (60.54%), Postives = 185/261 (70.88%), Query Frame = 0

Query: 4   TTGGGTTSSG-GGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGNSP 63
           ++GGG    G GG  G   SGL+R RSAPATWLEALLE+DEE+ LKPN  LT LL GNS 
Sbjct: 8   SSGGGDDGGGRGGGGGLSRSGLSRIRSAPATWLEALLEEDEEESLKPNLGLTDLLTGNSN 67

Query: 64  AL----GS-----PPDQGLFDANPNPNPSPAFARQNSSPAEFL--GEGFYSAYGVTSAKY 123
            L    GS     P +QGL+           F RQNS+PA+FL   +GF  ++G+  A Y
Sbjct: 68  DLPTSRGSFEFPIPVEQGLYQQG-------GFHRQNSTPADFLSGSDGFIQSFGI-QANY 127

Query: 124 ELPSPGLDISPTSKRGREVEAQNFHSKFSSQLKREES------GVSSLLDMEMEKLLEDS 183
           +  S  +D+SP SKR RE+EA     +F+SQ+K E+S      GVSS+ DM ME L+EDS
Sbjct: 128 DYLSGNIDVSPGSKRSREMEALFSSPEFTSQMKGEQSSGQVPTGVSSMSDMNMENLMEDS 187

Query: 184 VPCRVRAKRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKF 243
           V  RVRAKRGCATHPRSIAERVRRT+ISDRIRKLQE+VPNMDKQTNTADMLEEAVEYVK 
Sbjct: 188 VAFRVRAKRGCATHPRSIAERVRRTRISDRIRKLQELVPNMDKQTNTADMLEEAVEYVKV 247

Query: 244 LQRQIQELTEHQRGCKCMVKE 247
           LQRQIQELTE Q+ C C+ KE
Sbjct: 248 LQRQIQELTEEQKRCTCIPKE 260

BLAST of MC11g1458 vs. ExPASy Swiss-Prot
Match: Q66GR3 (Transcription factor bHLH130 OS=Arabidopsis thaliana OX=3702 GN=BHLH130 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 5.0e-30
Identity = 85/210 (40.48%), Postives = 119/210 (56.67%), Query Frame = 0

Query: 40  EDDEEDPLKPNQCLTQLLAGNSPALGSPPDQGLFDANPNPNPSPAFARQN-SSPAEFLGE 99
           E+DEE P   N         + P    P   G+    P   P   F   + + P+ F+  
Sbjct: 161 EEDEESPSNSNGLRRHCSLSSRP----PSSLGMLSQIPEIAPETNFPYSHWNDPSSFID- 220

Query: 100 GFYSAYGVTSAKYELPSPGLDI--SPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDM 159
                  ++S K E    G     +   + G  ++  + H      L +  S  S ++ +
Sbjct: 221 ------NLSSLKREAEDDGKLFLGAQNGESGNRMQLLSHH----LSLPKSSSTASDMVSV 280

Query: 160 EMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADML 219
           +    L+DSVPC++RAKRGCATHPRSIAERVRRT+IS+R+RKLQE+VPNMDKQTNT+DML
Sbjct: 281 DKYLQLQDSVPCKIRAKRGCATHPRSIAERVRRTRISERMRKLQELVPNMDKQTNTSDML 340

Query: 220 EEAVEYVKFLQRQIQELTEHQRGCKCMVKE 247
           + AV+Y+K LQRQ + L +++  CKCM KE
Sbjct: 341 DLAVDYIKDLQRQYKILNDNRANCKCMNKE 355

BLAST of MC11g1458 vs. ExPASy Swiss-Prot
Match: Q9C690 (Transcription factor bHLH122 OS=Arabidopsis thaliana OX=3702 GN=BHLH122 PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 8.0e-28
Identity = 59/86 (68.60%), Postives = 76/86 (88.37%), Query Frame = 0

Query: 157 EMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADML 216
           ++E+LL DS+PC++RAKRGCATHPRSIAERVRRTKIS+R+RKLQ++VPNMD QTNTADML
Sbjct: 291 DIEQLLSDSIPCKIRAKRGCATHPRSIAERVRRTKISERMRKLQDLVPNMDTQTNTADML 350

Query: 217 EEAVEYVKFLQRQIQELTEHQRGCKC 243
           + AV+Y+K LQ Q++ L E +  C+C
Sbjct: 351 DLAVQYIKDLQEQVKALEESRARCRC 376

BLAST of MC11g1458 vs. ExPASy Swiss-Prot
Match: Q8H102 (Transcription factor bHLH128 OS=Arabidopsis thaliana OX=3702 GN=BHLH128 PE=1 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 3.1e-24
Identity = 93/250 (37.20%), Postives = 129/250 (51.60%), Query Frame = 0

Query: 7   GGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGNSPALGS 66
           GG  SSG       +  LAR RS+PA +   L  D      K N  L Q  +  SP  GS
Sbjct: 129 GGGNSSG-------SYSLARQRSSPADFFTYLASD------KNNFSLNQPTSDYSPQGGS 188

Query: 67  PPDQGLFDAN-----PNPNPSPAFARQNSSPAEFLGEGFYSAYGVTSAKYE-----LPSP 126
              +G           N +        N +P        +SA    +A  +       S 
Sbjct: 189 NGGRGHSRLKSQLSFTNHDSLARINEVNETPVHDGSGHSFSAASFGAATTDSWDDGSGSI 248

Query: 127 GLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDMEMEKLLEDSVPCRVRAKRGC 186
           G  ++  SKR +++++  F     SQ         + +D  M+ L EDSVPC++RAKRGC
Sbjct: 249 GFTVTRPSKRSKDMDSGLF-----SQYSLPSDTSMNYMDNFMQ-LPEDSVPCKIRAKRGC 308

Query: 187 ATHPRSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQRQIQELTEH 246
           ATHPRSIAER RRT+IS +++KLQ++VPNMDKQT+ +DML+ AV+++K LQ Q+Q L + 
Sbjct: 309 ATHPRSIAERERRTRISGKLKKLQDLVPNMDKQTSYSDMLDLAVQHIKGLQHQLQNLKKD 359

BLAST of MC11g1458 vs. NCBI nr
Match: XP_022155250.1 (transcription factor bHLH80-like [Momordica charantia])

HSP 1 Score: 481 bits (1238), Expect = 4.25e-171
Identity = 246/246 (100.00%), Postives = 246/246 (100.00%), Query Frame = 0

Query: 1   MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN 60
           MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN
Sbjct: 1   MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN 60

Query: 61  SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFLGEGFYSAYGVTSAKYELPSPGLDI 120
           SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFLGEGFYSAYGVTSAKYELPSPGLDI
Sbjct: 61  SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFLGEGFYSAYGVTSAKYELPSPGLDI 120

Query: 121 SPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDMEMEKLLEDSVPCRVRAKRGCATHP 180
           SPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDMEMEKLLEDSVPCRVRAKRGCATHP
Sbjct: 121 SPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDMEMEKLLEDSVPCRVRAKRGCATHP 180

Query: 181 RSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQRQIQELTEHQRGC 240
           RSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQRQIQELTEHQRGC
Sbjct: 181 RSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQRQIQELTEHQRGC 240

Query: 241 KCMVKE 246
           KCMVKE
Sbjct: 241 KCMVKE 246

BLAST of MC11g1458 vs. NCBI nr
Match: XP_038891266.1 (transcription factor bHLH80-like [Benincasa hispida])

HSP 1 Score: 358 bits (918), Expect = 1.95e-122
Identity = 201/254 (79.13%), Postives = 210/254 (82.68%), Query Frame = 0

Query: 1   MQSTTGGGTT---SSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLL 60
           MQSTTG G T   SSGGG      +GLARFRSAPA WLEALLEDDEEDPLKP+ CLTQLL
Sbjct: 1   MQSTTGAGATGSRSSGGGG----GAGLARFRSAPAAWLEALLEDDEEDPLKPSHCLTQLL 60

Query: 61  AGNSPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFL-----GEGFYSAYGVTSAKYE 120
           A NS  L SP DQ LFD    PNPSPAF RQNSSPAEFL     GEGFY+AY + S    
Sbjct: 61  AANSSDLDSPADQPLFD----PNPSPAFHRQNSSPAEFLAASGIGEGFYTAYALNS---- 120

Query: 121 LPSPGLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDMEMEKLLEDSVPCRVRA 180
             SP LD+SPTSKR REV+AQNF  KFS QLKRE SGVSSL+DMEMEKLLEDSVPCRVRA
Sbjct: 121 --SPTLDVSPTSKRAREVDAQNFSPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRA 180

Query: 181 KRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQRQIQE 240
           KRGCATHPRSIAERVRRT+ISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQ+QIQE
Sbjct: 181 KRGCATHPRSIAERVRRTRISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQKQIQE 240

Query: 241 LTEHQRGCKCMVKE 246
           LTEHQR CKCMVKE
Sbjct: 241 LTEHQRRCKCMVKE 240

BLAST of MC11g1458 vs. NCBI nr
Match: XP_022925421.1 (transcription factor bHLH81 [Cucurbita moschata] >XP_023535232.1 transcription factor bHLH81 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 349 bits (895), Expect = 5.97e-119
Identity = 195/251 (77.69%), Postives = 205/251 (81.67%), Query Frame = 0

Query: 1   MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN 60
           MQSTTGG T S  GG  G    GLARFRSAPA WLEALLED+EED L+PNQCLTQLLAGN
Sbjct: 1   MQSTTGGATGSRSGG--GGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGN 60

Query: 61  SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFLG-----EGFYSAYGVTSAKYELPS 120
           S  L SP DQ LF+    PNPS  F RQNSSPAEFLG     EGFYS+Y + S      S
Sbjct: 61  SSDLDSPADQALFE----PNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINS------S 120

Query: 121 PGLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDMEMEKLLEDSVPCRVRAKRG 180
             L+ISPTSKRGREV+AQNF  KFS QLKRE SGVSSL+DMEMEKLLEDSVPCRVRAKRG
Sbjct: 121 AALEISPTSKRGREVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRG 180

Query: 181 CATHPRSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQRQIQELTE 240
           CATHPRSIAERVRRT+ISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQ+QIQELTE
Sbjct: 181 CATHPRSIAERVRRTRISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQKQIQELTE 239

Query: 241 HQRGCKCMVKE 246
           HQR C C VKE
Sbjct: 241 HQRRCTCTVKE 239

BLAST of MC11g1458 vs. NCBI nr
Match: KAG6592393.1 (Transcription factor basic helix-loop-helix 80, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 349 bits (895), Expect = 2.49e-118
Identity = 195/251 (77.69%), Postives = 205/251 (81.67%), Query Frame = 0

Query: 1   MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN 60
           MQSTTGG T S  GG  G    GLARFRSAPA WLEALLED+EED L+PNQCLTQLLAGN
Sbjct: 42  MQSTTGGATGSRSGG--GGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGN 101

Query: 61  SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFLG-----EGFYSAYGVTSAKYELPS 120
           S  L SP DQ LF+    PNPS  F RQNSSPAEFLG     EGFYS+Y + S      S
Sbjct: 102 SSDLDSPADQALFE----PNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINS------S 161

Query: 121 PGLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDMEMEKLLEDSVPCRVRAKRG 180
             L+ISPTSKRGREV+AQNF  KFS QLKRE SGVSSL+DMEMEKLLEDSVPCRVRAKRG
Sbjct: 162 AALEISPTSKRGREVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRG 221

Query: 181 CATHPRSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQRQIQELTE 240
           CATHPRSIAERVRRT+ISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQ+QIQELTE
Sbjct: 222 CATHPRSIAERVRRTRISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQKQIQELTE 280

Query: 241 HQRGCKCMVKE 246
           HQR C C VKE
Sbjct: 282 HQRRCTCTVKE 280

BLAST of MC11g1458 vs. NCBI nr
Match: XP_022973990.1 (transcription factor bHLH81 [Cucurbita maxima])

HSP 1 Score: 344 bits (883), Expect = 3.48e-117
Identity = 195/253 (77.08%), Postives = 206/253 (81.42%), Query Frame = 0

Query: 1   MQSTTGG--GTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLA 60
           MQSTTGG  G+ SSGGG        LARFRSAPA WLEALLED+EED L+PNQCLTQLLA
Sbjct: 1   MQSTTGGATGSRSSGGG--------LARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLA 60

Query: 61  GNSPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFLG-----EGFYSAYGVTSAKYEL 120
           GNS  L SP DQ LF+    PNPS  F RQNSSPAEFLG     EGFYS+Y + S     
Sbjct: 61  GNSSDLDSPADQALFE----PNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINS----- 120

Query: 121 PSPGLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDMEMEKLLEDSVPCRVRAK 180
            S  L+ISPTSKRGREV+AQNF  KFS QLKRE SGVSSL+DMEMEKLLEDSVPCRVRAK
Sbjct: 121 -SAALEISPTSKRGREVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAK 180

Query: 181 RGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQRQIQEL 240
           RGCATHPRSIAERVRRT+ISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQ+QIQEL
Sbjct: 181 RGCATHPRSIAERVRRTRISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQKQIQEL 235

Query: 241 TEHQRGCKCMVKE 246
           TEHQR C C VKE
Sbjct: 241 TEHQRRCTCTVKE 235

BLAST of MC11g1458 vs. ExPASy TrEMBL
Match: A0A6J1DLX6 (transcription factor bHLH80-like OS=Momordica charantia OX=3673 GN=LOC111022390 PE=4 SV=1)

HSP 1 Score: 481 bits (1238), Expect = 2.06e-171
Identity = 246/246 (100.00%), Postives = 246/246 (100.00%), Query Frame = 0

Query: 1   MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN 60
           MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN
Sbjct: 1   MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN 60

Query: 61  SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFLGEGFYSAYGVTSAKYELPSPGLDI 120
           SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFLGEGFYSAYGVTSAKYELPSPGLDI
Sbjct: 61  SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFLGEGFYSAYGVTSAKYELPSPGLDI 120

Query: 121 SPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDMEMEKLLEDSVPCRVRAKRGCATHP 180
           SPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDMEMEKLLEDSVPCRVRAKRGCATHP
Sbjct: 121 SPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDMEMEKLLEDSVPCRVRAKRGCATHP 180

Query: 181 RSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQRQIQELTEHQRGC 240
           RSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQRQIQELTEHQRGC
Sbjct: 181 RSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQRQIQELTEHQRGC 240

Query: 241 KCMVKE 246
           KCMVKE
Sbjct: 241 KCMVKE 246

BLAST of MC11g1458 vs. ExPASy TrEMBL
Match: A0A6J1EC61 (transcription factor bHLH81 OS=Cucurbita moschata OX=3662 GN=LOC111432717 PE=4 SV=1)

HSP 1 Score: 349 bits (895), Expect = 2.89e-119
Identity = 195/251 (77.69%), Postives = 205/251 (81.67%), Query Frame = 0

Query: 1   MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN 60
           MQSTTGG T S  GG  G    GLARFRSAPA WLEALLED+EED L+PNQCLTQLLAGN
Sbjct: 1   MQSTTGGATGSRSGG--GGSGGGLARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLAGN 60

Query: 61  SPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFLG-----EGFYSAYGVTSAKYELPS 120
           S  L SP DQ LF+    PNPS  F RQNSSPAEFLG     EGFYS+Y + S      S
Sbjct: 61  SSDLDSPADQALFE----PNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINS------S 120

Query: 121 PGLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDMEMEKLLEDSVPCRVRAKRG 180
             L+ISPTSKRGREV+AQNF  KFS QLKRE SGVSSL+DMEMEKLLEDSVPCRVRAKRG
Sbjct: 121 AALEISPTSKRGREVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKRG 180

Query: 181 CATHPRSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQRQIQELTE 240
           CATHPRSIAERVRRT+ISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQ+QIQELTE
Sbjct: 181 CATHPRSIAERVRRTRISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQKQIQELTE 239

Query: 241 HQRGCKCMVKE 246
           HQR C C VKE
Sbjct: 241 HQRRCTCTVKE 239

BLAST of MC11g1458 vs. ExPASy TrEMBL
Match: A0A6J1ICS6 (transcription factor bHLH81 OS=Cucurbita maxima OX=3661 GN=LOC111472602 PE=4 SV=1)

HSP 1 Score: 344 bits (883), Expect = 1.68e-117
Identity = 195/253 (77.08%), Postives = 206/253 (81.42%), Query Frame = 0

Query: 1   MQSTTGG--GTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLA 60
           MQSTTGG  G+ SSGGG        LARFRSAPA WLEALLED+EED L+PNQCLTQLLA
Sbjct: 1   MQSTTGGATGSRSSGGG--------LARFRSAPAAWLEALLEDEEEDLLRPNQCLTQLLA 60

Query: 61  GNSPALGSPPDQGLFDANPNPNPSPAFARQNSSPAEFLG-----EGFYSAYGVTSAKYEL 120
           GNS  L SP DQ LF+    PNPS  F RQNSSPAEFLG     EGFYS+Y + S     
Sbjct: 61  GNSSDLDSPADQALFE----PNPSAGFQRQNSSPAEFLGASGIGEGFYSSYAINS----- 120

Query: 121 PSPGLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDMEMEKLLEDSVPCRVRAK 180
            S  L+ISPTSKRGREV+AQNF  KFS QLKRE SGVSSL+DMEMEKLLEDSVPCRVRAK
Sbjct: 121 -SAALEISPTSKRGREVDAQNFPPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAK 180

Query: 181 RGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQRQIQEL 240
           RGCATHPRSIAERVRRT+ISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQ+QIQEL
Sbjct: 181 RGCATHPRSIAERVRRTRISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQKQIQEL 235

Query: 241 TEHQRGCKCMVKE 246
           TEHQR C C VKE
Sbjct: 241 TEHQRRCTCTVKE 235

BLAST of MC11g1458 vs. ExPASy TrEMBL
Match: A0A1S3CBY9 (transcription factor bHLH80 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498724 PE=4 SV=1)

HSP 1 Score: 337 bits (863), Expect = 2.38e-114
Identity = 190/252 (75.40%), Postives = 200/252 (79.37%), Query Frame = 0

Query: 1   MQSTTGGGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGN 60
           MQSTTG   T++          GLARFRSAPA WLEALLEDDEEDPLKPN CLTQLLA N
Sbjct: 1   MQSTTGAAATATASSRTSGGGPGLARFRSAPAAWLEALLEDDEEDPLKPNPCLTQLLAAN 60

Query: 61  SPALGSPP-DQGLFDANPNPNPSPAFARQNSSPAEFLG-----EGFYSAYGVTSAKYELP 120
           S  L S P D  LFD    PNPSPAF RQNSSP EFL      EGFY++Y + S      
Sbjct: 61  SSDLVSAPGDHPLFD----PNPSPAFHRQNSSPPEFLAPSGIAEGFYTSYPLNS------ 120

Query: 121 SPGLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDMEMEKLLEDSVPCRVRAKR 180
           SP LDISPTSK   +V+AQNF  KFS QLKRE SGVSSL+DMEMEKLLEDSVPCRVRAKR
Sbjct: 121 SPTLDISPTSKPATDVDAQNFFPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRAKR 180

Query: 181 GCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQRQIQELT 240
           GCATHPRSIAERVRRT+ISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQ+QIQELT
Sbjct: 181 GCATHPRSIAERVRRTRISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQKQIQELT 240

Query: 241 EHQRGCKCMVKE 246
           EHQR CKCMVKE
Sbjct: 241 EHQRRCKCMVKE 242

BLAST of MC11g1458 vs. ExPASy TrEMBL
Match: A0A0A0KY01 (BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G652220 PE=4 SV=1)

HSP 1 Score: 335 bits (858), Expect = 1.47e-113
Identity = 191/254 (75.20%), Postives = 203/254 (79.92%), Query Frame = 0

Query: 1   MQSTTGGGTTSSGGGDLGPRA--SGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLA 60
           MQSTTG  +T++        A  +GLARFRSAPA WLEALLEDDEEDPLKPN CLTQLLA
Sbjct: 1   MQSTTGAASTATATASSRTSAGGAGLARFRSAPAAWLEALLEDDEEDPLKPNPCLTQLLA 60

Query: 61  GNSPALGSPP-DQGLFDANPNPNPSPAFARQNSSPAEFLG-----EGFYSAYGVTSAKYE 120
            NS  L S P D  LFD    PNPSPAF RQNSSP EFL      EGFY++Y + S    
Sbjct: 61  ANSSDLDSAPADHPLFD----PNPSPAFHRQNSSPPEFLAPSGIAEGFYTSYPLNS---- 120

Query: 121 LPSPGLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDMEMEKLLEDSVPCRVRA 180
             SP LDISPTSK   +V+AQNF  KFS QLKRE SGVSSL+DMEMEKLLEDSVPCRVRA
Sbjct: 121 --SPTLDISPTSKPSTDVDAQNFFPKFSPQLKREGSGVSSLIDMEMEKLLEDSVPCRVRA 180

Query: 181 KRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQRQIQE 240
           KRGCATHPRSIAERVRRT+ISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQ+QIQE
Sbjct: 181 KRGCATHPRSIAERVRRTRISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQKQIQE 240

Query: 241 LTEHQRGCKCMVKE 246
           LTEHQR CKCMVKE
Sbjct: 241 LTEHQRRCKCMVKE 244

BLAST of MC11g1458 vs. TAIR 10
Match: AT1G35460.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 261.2 bits (666), Expect = 8.7e-70
Identity = 158/268 (58.96%), Postives = 189/268 (70.52%), Query Frame = 0

Query: 1   MQSTTGGGTTSSGGGDLGPRA--SGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLA 60
           MQST   G +S GGG  G     SGL+R RSAPATW+E LLE+DEE+ LKPN CLT+LL 
Sbjct: 1   MQSTHISGGSSGGGGGGGGEVSRSGLSRIRSAPATWIETLLEEDEEEGLKPNLCLTELLT 60

Query: 61  GNSPALG------------SPPDQGLFDANPNPNPSPAFARQNSSPAEFLG------EGF 120
           GN+ + G            S  +QGL+    N +    F RQNSSPA+FL       +G+
Sbjct: 61  GNNNSGGVITSRDDSFEFLSSVEQGLY----NHHQGGGFHRQNSSPADFLSGSGSGTDGY 120

Query: 121 YSAYGVTSAKYELPSPGLDISPTSKRGREVEAQNFHSKFSSQLKREE--SGVSSLLDMEM 180
           +S +G+  A Y+  S  +DISPT KR R++E Q     FSSQLK E+   G+S ++DM M
Sbjct: 121 FSNFGI-PANYDYLSTNVDISPT-KRSRDMETQ-----FSSQLKEEQMSGGISGMMDMNM 180

Query: 181 EKLLEDSVPCRVRAKRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEE 240
           +K+ EDSVPCRVRAKRGCATHPRSIAERVRRT+ISDRIR+LQE+VPNMDKQTNTADMLEE
Sbjct: 181 DKIFEDSVPCRVRAKRGCATHPRSIAERVRRTRISDRIRRLQELVPNMDKQTNTADMLEE 240

Query: 241 AVEYVKFLQRQIQELTEHQRGCKCMVKE 247
           AVEYVK LQ QIQELTE Q+ CKC  KE
Sbjct: 241 AVEYVKALQSQIQELTEQQKRCKCKPKE 257

BLAST of MC11g1458 vs. TAIR 10
Match: AT4G09180.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 258.1 bits (658), Expect = 7.3e-69
Identity = 158/261 (60.54%), Postives = 185/261 (70.88%), Query Frame = 0

Query: 4   TTGGGTTSSG-GGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGNSP 63
           ++GGG    G GG  G   SGL+R RSAPATWLEALLE+DEE+ LKPN  LT LL GNS 
Sbjct: 8   SSGGGDDGGGRGGGGGLSRSGLSRIRSAPATWLEALLEEDEEESLKPNLGLTDLLTGNSN 67

Query: 64  AL----GS-----PPDQGLFDANPNPNPSPAFARQNSSPAEFL--GEGFYSAYGVTSAKY 123
            L    GS     P +QGL+           F RQNS+PA+FL   +GF  ++G+  A Y
Sbjct: 68  DLPTSRGSFEFPIPVEQGLYQQG-------GFHRQNSTPADFLSGSDGFIQSFGI-QANY 127

Query: 124 ELPSPGLDISPTSKRGREVEAQNFHSKFSSQLKREES------GVSSLLDMEMEKLLEDS 183
           +  S  +D+SP SKR RE+EA     +F+SQ+K E+S      GVSS+ DM ME L+EDS
Sbjct: 128 DYLSGNIDVSPGSKRSREMEALFSSPEFTSQMKGEQSSGQVPTGVSSMSDMNMENLMEDS 187

Query: 184 VPCRVRAKRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKF 243
           V  RVRAKRGCATHPRSIAERVRRT+ISDRIRKLQE+VPNMDKQTNTADMLEEAVEYVK 
Sbjct: 188 VAFRVRAKRGCATHPRSIAERVRRTRISDRIRKLQELVPNMDKQTNTADMLEEAVEYVKV 247

Query: 244 LQRQIQELTEHQRGCKCMVKE 247
           LQRQIQELTE Q+ C C+ KE
Sbjct: 248 LQRQIQELTEEQKRCTCIPKE 260

BLAST of MC11g1458 vs. TAIR 10
Match: AT2G42280.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 132.9 bits (333), Expect = 3.6e-31
Identity = 85/210 (40.48%), Postives = 119/210 (56.67%), Query Frame = 0

Query: 40  EDDEEDPLKPNQCLTQLLAGNSPALGSPPDQGLFDANPNPNPSPAFARQN-SSPAEFLGE 99
           E+DEE P   N         + P    P   G+    P   P   F   + + P+ F+  
Sbjct: 161 EEDEESPSNSNGLRRHCSLSSRP----PSSLGMLSQIPEIAPETNFPYSHWNDPSSFID- 220

Query: 100 GFYSAYGVTSAKYELPSPGLDI--SPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDM 159
                  ++S K E    G     +   + G  ++  + H      L +  S  S ++ +
Sbjct: 221 ------NLSSLKREAEDDGKLFLGAQNGESGNRMQLLSHH----LSLPKSSSTASDMVSV 280

Query: 160 EMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADML 219
           +    L+DSVPC++RAKRGCATHPRSIAERVRRT+IS+R+RKLQE+VPNMDKQTNT+DML
Sbjct: 281 DKYLQLQDSVPCKIRAKRGCATHPRSIAERVRRTRISERMRKLQELVPNMDKQTNTSDML 340

Query: 220 EEAVEYVKFLQRQIQELTEHQRGCKCMVKE 247
           + AV+Y+K LQRQ + L +++  CKCM KE
Sbjct: 341 DLAVDYIKDLQRQYKILNDNRANCKCMNKE 355

BLAST of MC11g1458 vs. TAIR 10
Match: AT1G51140.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 125.6 bits (314), Expect = 5.7e-29
Identity = 59/86 (68.60%), Postives = 76/86 (88.37%), Query Frame = 0

Query: 157 EMEKLLEDSVPCRVRAKRGCATHPRSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADML 216
           ++E+LL DS+PC++RAKRGCATHPRSIAERVRRTKIS+R+RKLQ++VPNMD QTNTADML
Sbjct: 291 DIEQLLSDSIPCKIRAKRGCATHPRSIAERVRRTKISERMRKLQDLVPNMDTQTNTADML 350

Query: 217 EEAVEYVKFLQRQIQELTEHQRGCKC 243
           + AV+Y+K LQ Q++ L E +  C+C
Sbjct: 351 DLAVQYIKDLQEQVKALEESRARCRC 376

BLAST of MC11g1458 vs. TAIR 10
Match: AT1G05805.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 113.6 bits (283), Expect = 2.2e-25
Identity = 93/250 (37.20%), Postives = 129/250 (51.60%), Query Frame = 0

Query: 7   GGTTSSGGGDLGPRASGLARFRSAPATWLEALLEDDEEDPLKPNQCLTQLLAGNSPALGS 66
           GG  SSG       +  LAR RS+PA +   L  D      K N  L Q  +  SP  GS
Sbjct: 129 GGGNSSG-------SYSLARQRSSPADFFTYLASD------KNNFSLNQPTSDYSPQGGS 188

Query: 67  PPDQGLFDAN-----PNPNPSPAFARQNSSPAEFLGEGFYSAYGVTSAKYE-----LPSP 126
              +G           N +        N +P        +SA    +A  +       S 
Sbjct: 189 NGGRGHSRLKSQLSFTNHDSLARINEVNETPVHDGSGHSFSAASFGAATTDSWDDGSGSI 248

Query: 127 GLDISPTSKRGREVEAQNFHSKFSSQLKREESGVSSLLDMEMEKLLEDSVPCRVRAKRGC 186
           G  ++  SKR +++++  F     SQ         + +D  M+ L EDSVPC++RAKRGC
Sbjct: 249 GFTVTRPSKRSKDMDSGLF-----SQYSLPSDTSMNYMDNFMQ-LPEDSVPCKIRAKRGC 308

Query: 187 ATHPRSIAERVRRTKISDRIRKLQEVVPNMDKQTNTADMLEEAVEYVKFLQRQIQELTEH 246
           ATHPRSIAER RRT+IS +++KLQ++VPNMDKQT+ +DML+ AV+++K LQ Q+Q L + 
Sbjct: 309 ATHPRSIAERERRTRISGKLKKLQDLVPNMDKQTSYSDMLDLAVQHIKGLQHQLQNLKKD 359

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C8P81.2e-6858.96Transcription factor bHLH80 OS=Arabidopsis thaliana OX=3702 GN=BHLH80 PE=2 SV=1[more]
Q9M0R01.0e-6760.54Transcription factor bHLH81 OS=Arabidopsis thaliana OX=3702 GN=BHLH81 PE=2 SV=1[more]
Q66GR35.0e-3040.48Transcription factor bHLH130 OS=Arabidopsis thaliana OX=3702 GN=BHLH130 PE=1 SV=... [more]
Q9C6908.0e-2868.60Transcription factor bHLH122 OS=Arabidopsis thaliana OX=3702 GN=BHLH122 PE=1 SV=... [more]
Q8H1023.1e-2437.20Transcription factor bHLH128 OS=Arabidopsis thaliana OX=3702 GN=BHLH128 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
XP_022155250.14.25e-171100.00transcription factor bHLH80-like [Momordica charantia][more]
XP_038891266.11.95e-12279.13transcription factor bHLH80-like [Benincasa hispida][more]
XP_022925421.15.97e-11977.69transcription factor bHLH81 [Cucurbita moschata] >XP_023535232.1 transcription f... [more]
KAG6592393.12.49e-11877.69Transcription factor basic helix-loop-helix 80, partial [Cucurbita argyrosperma ... [more]
XP_022973990.13.48e-11777.08transcription factor bHLH81 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1DLX62.06e-171100.00transcription factor bHLH80-like OS=Momordica charantia OX=3673 GN=LOC111022390 ... [more]
A0A6J1EC612.89e-11977.69transcription factor bHLH81 OS=Cucurbita moschata OX=3662 GN=LOC111432717 PE=4 S... [more]
A0A6J1ICS61.68e-11777.08transcription factor bHLH81 OS=Cucurbita maxima OX=3661 GN=LOC111472602 PE=4 SV=... [more]
A0A1S3CBY92.38e-11475.40transcription factor bHLH80 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498724 P... [more]
A0A0A0KY011.47e-11375.20BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G652220 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT1G35460.18.7e-7058.96basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT4G09180.17.3e-6960.54basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT2G42280.13.6e-3140.48basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G51140.15.7e-2968.60basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G05805.12.2e-2537.20basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 216..236
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..16
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 73..89
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 58..90
NoneNo IPR availablePANTHERPTHR16223:SF225TRANSCRIPTION FACTOR BHLH80coord: 12..244
NoneNo IPR availablePANTHERPTHR16223TRANSCRIPTION FACTOR BHLH83-RELATEDcoord: 12..244
NoneNo IPR availableCDDcd11393bHLH_AtbHLH_likecoord: 181..233
e-value: 2.27878E-23
score: 87.2367
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 182..232
e-value: 2.6E-13
score: 60.2
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 182..227
e-value: 3.5E-10
score: 39.7
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 176..226
score: 14.64637
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3D4.10.280.10coord: 177..246
e-value: 2.7E-16
score: 61.4
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 179..241

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC11g1458.1MC11g1458.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006357 regulation of transcription by RNA polymerase II
cellular_component GO:0005634 nucleus
molecular_function GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding