MC11g1226 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g1226 vs. ExPASy Swiss-Prot
Match: Q17438 (DnaJ homolog subfamily B member 1 OS=Caenorhabditis elegans OX=6239 GN=dnj-1 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 4.4e-08
Identity = 31/82 (37.80%), Postives = 47/82 (57.32%), Query Frame = 0

Query: 285 TANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ 344
           T+ + E  E +    D      Y+IL +++  S ++I+K Y K++L +HPDKC  P A +
Sbjct: 122 TSEQKELVERIRHCKDY-----YEILKIDKKASDDDIRKEYRKLALKLHPDKCRAPHATE 181

Query: 345 AFIRLNNAFKELQDPDKRKALD 367
           AF  L NA+  L D DKR+  D
Sbjct: 182 AFKALGNAYAVLSDTDKRRQYD 198

BLAST of MC11g1226 vs. ExPASy Swiss-Prot
Match: P56101 (DnaJ homolog subfamily C member 5 OS=Tetronarce californica OX=7787 GN=dnajc5 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 9.7e-08
Identity = 32/75 (42.67%), Postives = 46/75 (61.33%), Query Frame = 0

Query: 293 EEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDK-CSHPQANQAFIRLNN 352
           ++  R +   GDS Y +LGL++N S E+IKK Y K++L  HPDK   +P+A++ F  +NN
Sbjct: 3   DQRQRSLSTSGDSLYIVLGLDKNASPEDIKKSYRKLALKYHPDKNPDNPEASEKFKEINN 62

Query: 353 AFKELQDPDKRKALD 367
           A   L D  KR   D
Sbjct: 63  AHAILTDATKRNIYD 77

BLAST of MC11g1226 vs. ExPASy Swiss-Prot
Match: Q9CZJ9 (DnaJ homolog subfamily C member 18 OS=Mus musculus OX=10090 GN=Dnajc18 PE=2 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.3e-07
Identity = 34/74 (45.95%), Postives = 44/74 (59.46%), Query Frame = 0

Query: 307 YDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFIRLNNAFKELQDPDKRKALD 366
           YDILG++ N S E +KK Y K++L  HPDK   P A +AF  + NAF  L +PDKR   D
Sbjct: 84  YDILGVSHNASDEELKKAYKKLALKFHPDKNCAPGATEAFKAIGNAFAVLSNPDKRLRYD 143

Query: 367 DKIKLKEEQEQFKV 381
           +     +EQ  F V
Sbjct: 144 E---YGDEQVTFTV 154

BLAST of MC11g1226 vs. ExPASy Swiss-Prot
Match: Q4V893 (GPALPP motifs-containing protein 1 OS=Rattus norvegicus OX=10116 GN=Gpalpp1 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.3e-07
Identity = 57/182 (31.32%), Postives = 85/182 (46.70%), Query Frame = 0

Query: 403 EELLANTEDDVKVEPKRDEWMTTLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPLDR 462
           +E L   +DD      R+ WMT LPPE K    +    F + + +  GD SVWTDTP DR
Sbjct: 180 KEKLTKGDDDSPKPVTRESWMTELPPEMK-DFGLGPRTFKRRADDKSGDRSVWTDTPADR 239

Query: 463 AQKAKTSYLEAYNEATALASNEEAKKHSSE----SALVDKYNKEKRSKSLIEKHREETRS 522
            +KAK    E      +L+  +E    S      +  V  YN+ KRS+SL++ H +  ++
Sbjct: 240 ERKAK----EIQEARKSLSKKDEENMLSGRDKRLAEQVSSYNESKRSESLMDIHHKRLKT 299

Query: 523 RAKKKSKQVAEKGDDWVGKHPWKPWDREKDLTAGRKTVNFDPE------NMAQGLGSRFS 575
           +A +   +  E+           P+DR+KDL   R    FD          ++ L +RFS
Sbjct: 300 KAAEDKNRHQER----------TPFDRDKDLKVNR----FDEAQKKALIKKSRELNTRFS 342

BLAST of MC11g1226 vs. ExPASy Swiss-Prot
Match: Q2QUP1 (DnaJ protein ERDJ7 OS=Oryza sativa subsp. japonica OX=39947 GN=ERDJ7 PE=3 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 2.8e-07
Identity = 35/110 (31.82%), Postives = 56/110 (50.91%), Query Frame = 0

Query: 265 EEDNELFVGPPPPAMVAEAETANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKR 324
           E  N +   PPP  ++              VS  +  E D  YD+LG+ ++ ++  IKK 
Sbjct: 9   EGSNSMAAAPPPRLLLLVVLL------LVPVSNAIYCEEDDCYDLLGVKQDANVSEIKKA 68

Query: 325 YWKMSLLVHPDKCSHPQANQAFIRLNNAFKELQDPDKRKALDDKIKLKEE 375
           Y+K+SL  HPDK   P++ + F+++ NA++ L+D   R   D  I   EE
Sbjct: 69  YYKLSLKHHPDKNPDPESRKLFVKIANAYEILKDESTRGQYDYAIAHPEE 112

BLAST of MC11g1226 vs. NCBI nr
Match: XP_022156581.1 (uncharacterized protein LOC111023459 [Momordica charantia])

HSP 1 Score: 1091 bits (2821), Expect = 0.0
Identity = 580/581 (99.83%), Postives = 581/581 (100.00%), Query Frame = 0

Query: 1   MGSGRKKWRTDDSEPSSSSPSSDDDSDTSASRSRRHHRGGGSRREKDKKRESRKRKDRMR 60
           MGSGRKKWRTDDSEPSSSSPSSDDDSDTSASRSRRHHRGGGSRREKDKKRESRKRKDRMR
Sbjct: 1   MGSGRKKWRTDDSEPSSSSPSSDDDSDTSASRSRRHHRGGGSRREKDKKRESRKRKDRMR 60

Query: 61  ERDRKRRKKKSRREGKRKRDYFSESDDGSSGESELEKVRIEPEVVVRQLLKEFPDVGDDL 120
           ERDRKRRKKKSRREGKRKRDYFSESDDGSSGESELEKVRIEPEVVVRQLLKEFPDVGDDL
Sbjct: 61  ERDRKRRKKKSRREGKRKRDYFSESDDGSSGESELEKVRIEPEVVVRQLLKEFPDVGDDL 120

Query: 121 EQLLKMIDDGQAVDISGISERSLTKRLKKLFRSLNLEETQDRVFLLPSNGRSTLELIGPI 180
           EQLLKMIDDGQAVDISGISERSLTKRLKKLFRSLNLEETQDRVFLLPSNGRSTLELIGPI
Sbjct: 121 EQLLKMIDDGQAVDISGISERSLTKRLKKLFRSLNLEETQDRVFLLPSNGRSTLELIGPI 180

Query: 181 IQFHMEQKRNQFDHSVTLDNKSSPPSNMETTQAREDNMSELACPDGEASGPKRRMLGPEM 240
           IQFHMEQKRNQFDHSVTLDNKSSPPSNMETTQAREDNMSELACPDGEASGPKRRMLGPEM
Sbjct: 181 IQFHMEQKRNQFDHSVTLDNKSSPPSNMETTQAREDNMSELACPDGEASGPKRRMLGPEM 240

Query: 241 PSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAETANEAERFEEVSRIMD 300
           PSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAETANEAERFEEVSRIMD
Sbjct: 241 PSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAETANEAERFEEVSRIMD 300

Query: 301 VEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFIRLNNAFKELQDPD 360
           VEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFIRLNNAFKELQDPD
Sbjct: 301 VEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFIRLNNAFKELQDPD 360

Query: 361 KRKALDDKIKLKEEQEQFKVELRGMREAAKWRQSQGISMDGDEELLANTEDDVKVEPKRD 420
           KRKALDDKIKLKEEQEQFKVELRGMREAAKWRQSQGISMDGDEELLANTEDDVKVEPKRD
Sbjct: 361 KRKALDDKIKLKEEQEQFKVELRGMREAAKWRQSQGISMDGDEELLANTEDDVKVEPKRD 420

Query: 421 EWMTTLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPLDRAQKAKTSYLEAYNEATAL 480
           EWMTTLPPERKPG+TMQSSRFSKSSKEGRGDTSVWTDTPLDRAQKAKTSYLEAYNEATAL
Sbjct: 421 EWMTTLPPERKPGITMQSSRFSKSSKEGRGDTSVWTDTPLDRAQKAKTSYLEAYNEATAL 480

Query: 481 ASNEEAKKHSSESALVDKYNKEKRSKSLIEKHREETRSRAKKKSKQVAEKGDDWVGKHPW 540
           ASNEEAKKHSSESALVDKYNKEKRSKSLIEKHREETRSRAKKKSKQVAEKGDDWVGKHPW
Sbjct: 481 ASNEEAKKHSSESALVDKYNKEKRSKSLIEKHREETRSRAKKKSKQVAEKGDDWVGKHPW 540

Query: 541 KPWDREKDLTAGRKTVNFDPENMAQGLGSRFSSGSYQRNFL 581
           KPWDREKDLTAGRKTVNFDPENMAQGLGSRFSSGSYQRNFL
Sbjct: 541 KPWDREKDLTAGRKTVNFDPENMAQGLGSRFSSGSYQRNFL 581

BLAST of MC11g1226 vs. NCBI nr
Match: XP_038898675.1 (DNA ligase 1 [Benincasa hispida])

HSP 1 Score: 936 bits (2418), Expect = 0.0
Identity = 518/597 (86.77%), Postives = 548/597 (91.79%), Query Frame = 0

Query: 1   MGSGRKKWRTDDS-EPSSSSPSSDDDSDTSASRSRRHHRGGGSRREKDKKRESR------ 60
           MGS RKK RT DS EPSSSSPSS DDSD+S+ RSRRHHR G SRREKDK+RESR      
Sbjct: 1   MGSRRKKKRTVDSDEPSSSSPSSSDDSDSSSMRSRRHHRSGSSRREKDKERESRSRDRER 60

Query: 61  KRKDRMRERDRKRRKKKSRREGKRKRD-YFSESDDGS--SGES-----ELEKVRI-EPEV 120
           KRKDR RERDRKRRKKK RRE KRKRD Y SESD+ S  SG+S     +  KV+I EPEV
Sbjct: 61  KRKDRNRERDRKRRKKKLRRESKRKRDDYGSESDNESPHSGDSVDSRSDRRKVKIIEPEV 120

Query: 121 VVRQLLKEFPDVGDDLEQLLKMIDDGQAVDISGISERSLTKRLKKLFRSLNLEETQDRVF 180
           +VRQLLKEFPDVG+DL+QLLKMIDDGQAVDISGISERSL KRLKKLF SLNLEE+Q+RVF
Sbjct: 121 IVRQLLKEFPDVGNDLKQLLKMIDDGQAVDISGISERSLAKRLKKLFVSLNLEESQERVF 180

Query: 181 LLPSNGRSTLELIGPIIQFHMEQKRNQFDHSVTLDNKSSPPSNMETTQAREDNMSELACP 240
           LLPSN R TLE+IGP+I+ HMEQK NQ DHSVT+D+KSSPP NMETTQARED+MSELA P
Sbjct: 181 LLPSNVRPTLEVIGPLIETHMEQKSNQVDHSVTVDDKSSPPLNMETTQAREDDMSELAYP 240

Query: 241 DGEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAE 300
           DGEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEED+ELFVGPPPPAMVAEAE
Sbjct: 241 DGEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMVAEAE 300

Query: 301 TANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ 360
           TANEAERFEEV+RIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ
Sbjct: 301 TANEAERFEEVTRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ 360

Query: 361 AFIRLNNAFKELQDPDKRKALDDKIKLKEEQEQFKVELRGMREAAKWRQSQGISMDGDEE 420
           AFIRLNNAFKELQDPDKRKALDDKIK KEEQEQFKVELRGMREAAKWR+SQGISM+GDEE
Sbjct: 361 AFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDEE 420

Query: 421 LLANTEDDVKVEPKRDEWMTTLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPLDRAQ 480
           LLAN +D+VKVEPKRDEWMT+LPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTP DRAQ
Sbjct: 421 LLANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPFDRAQ 480

Query: 481 KAKTSYLEAYNEATALASNEEAKKHSSESALVDKYNKEKRSKSLIEKHREETRSRAKKKS 540
           KAK SYLEAYNEA ALASNEEAKK+SSESALVDKYN+EKRSKSL+EKHRE  +SR KKKS
Sbjct: 481 KAKMSYLEAYNEAAALASNEEAKKYSSESALVDKYNQEKRSKSLVEKHREGAKSRGKKKS 540

Query: 541 KQVAEKGDDWVGKHPWKPWDREKDLTAGRKTVNFDPENMAQGLGSRFSSGSYQRNFL 581
           KQV EK DDWVGKHPWKPWDREKDLTAGRKT+NFDPENMAQGLGSRFSSGSYQRNFL
Sbjct: 541 KQVVEKEDDWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQRNFL 597

BLAST of MC11g1226 vs. NCBI nr
Match: XP_022926268.1 (DNA ligase 1 [Cucurbita moschata])

HSP 1 Score: 912 bits (2356), Expect = 0.0
Identity = 501/597 (83.92%), Postives = 537/597 (89.95%), Query Frame = 0

Query: 1   MGSGRKKWRTDDSEPSSSSPSSDDDSDTSASRSRRHHRGGGSRREKDKKRESR------K 60
           MGSGRKK RT+ SEPSSSSPSS D+SD+S+ RSRRH R G SRREKDKKRESR      K
Sbjct: 1   MGSGRKKKRTECSEPSSSSPSSSDNSDSSSMRSRRHRRSGSSRREKDKKRESRIRDRERK 60

Query: 61  RKDRMRERDRKRRKKKSRREGKRKRD-YFSESD-----DGSSGESELEKVR---IEPEVV 120
           RKDR RERDRK RKKK RRE KRKRD Y SESD      G+S +SE E+ +   +EPEVV
Sbjct: 61  RKDRNRERDRKMRKKKLRREAKRKRDDYGSESDAESSRSGNSSDSEFERRKLRSVEPEVV 120

Query: 121 VRQLLKEFPDVGDDLEQLLKMIDDGQAVDISGISERSLTKRLKKLFRSLNLEETQDRVFL 180
           VR+LLKEFPDVG+DL+QLL MIDDGQAVDISGISER+L KRLKKLF SLNLEE+Q+RVFL
Sbjct: 121 VRELLKEFPDVGNDLKQLLSMIDDGQAVDISGISERTLAKRLKKLFLSLNLEESQERVFL 180

Query: 181 LPSNGRSTLELIGPIIQFHMEQKRNQFDHSVTLDNKSSPPSNMETTQAREDNMSELACPD 240
           LPSNG  TLE IGP+IQ HMEQK+NQFDHSVT DNKSSPP N ETTQ+ +DN+S+L  PD
Sbjct: 181 LPSNGHPTLEAIGPLIQTHMEQKKNQFDHSVTPDNKSSPPLNTETTQSSKDNVSDLTHPD 240

Query: 241 GEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAET 300
           GEA+GPKRRMLGPEMPSAELLAAAAKLTEAQTELRE ELEED+ELFVGPPPPAMVAEAET
Sbjct: 241 GEATGPKRRMLGPEMPSAELLAAAAKLTEAQTELREVELEEDHELFVGPPPPAMVAEAET 300

Query: 301 ANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA 360
           ANEAERFEEV+RIMDVEGDSPYDI GLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA
Sbjct: 301 ANEAERFEEVARIMDVEGDSPYDIFGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA 360

Query: 361 FIRLNNAFKELQDPDKRKALDDKIKLKEEQEQFKVELRGMREAAKWRQSQGISMDGDEEL 420
           FIRLNNAFKELQDPDKRKALDDKIK KEEQEQFKVELRGMREAAKWR+SQGISM+GD+EL
Sbjct: 361 FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDDEL 420

Query: 421 LANTEDDVKVEPKRDEWMTTLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPLDRAQK 480
           LAN +D+VKVEPKRDEWMT+LPPERKPGMTMQSSRFSK SKEGRGDTSVWTDTP DRAQK
Sbjct: 421 LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKGSKEGRGDTSVWTDTPSDRAQK 480

Query: 481 AKTSYLEAYNEATALASNEEAKKHSSESALVDKYNKEKRSKSLIEKHREETRSRAKKKSK 540
           AKTSYLEAYNEA ALAS+EEAKK+SSESALVDKYN+EKRSK LIEKHRE  RS  KKKSK
Sbjct: 481 AKTSYLEAYNEAAALASSEEAKKNSSESALVDKYNREKRSKPLIEKHREGARSSGKKKSK 540

Query: 541 Q-VAEKGDDWVGKHPWKPWDREKDLTAGRKTVNFDPENMAQGLGSRFSSGSYQRNFL 581
           Q V EK ++WVGKHPWKPWDREKDLTAGRKT+N DPENMAQGLGSRFSSGSYQRNFL
Sbjct: 541 QQVGEKEEEWVGKHPWKPWDREKDLTAGRKTINLDPENMAQGLGSRFSSGSYQRNFL 597

BLAST of MC11g1226 vs. NCBI nr
Match: KAG7011072.1 (hypothetical protein SDJN02_27870, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 911 bits (2355), Expect = 0.0
Identity = 503/598 (84.11%), Postives = 538/598 (89.97%), Query Frame = 0

Query: 1   MGSGRKKWRTDDSEPSSSSPSSDDDSDTSASRSRRHHRGGGSRREKDKKRESR------K 60
           MGSGRKK RT+ SEPSSSSPSS D+SD+S+ RSRRH R G SRREKDKKRESR      K
Sbjct: 119 MGSGRKKKRTECSEPSSSSPSSSDNSDSSSMRSRRHRRSGSSRREKDKKRESRIRDRERK 178

Query: 61  RKDRMRERDRKRRKKKSRREGKRKRD-YFSESD-----DGSSGESELEKVR---IEPEVV 120
           RKDR RERDRK RKKK RRE KRKRD Y SESD      G+S +SE E+ +   +EPEVV
Sbjct: 179 RKDRNRERDRKMRKKKLRREAKRKRDDYGSESDAESSRSGNSSDSEFERRKLRSVEPEVV 238

Query: 121 VRQLLKEFPDVGDDLEQLLKMIDDGQAVDISGISERSLTKRLKKLFRSLNLEETQDRVFL 180
           VR+LLKEFPDVG+DL+QLL MIDDGQAVDISGISER+L KRLKKLF SLNLEE+Q+RVFL
Sbjct: 239 VRELLKEFPDVGNDLKQLLSMIDDGQAVDISGISERTLAKRLKKLFLSLNLEESQERVFL 298

Query: 181 LPSNGRSTLELIGPIIQFHMEQKRNQFDHSVTLDNKSSPPSNMETTQAREDNMSELACPD 240
           LPSNG  TLE IGP+IQ HMEQK+NQFDHSVT DNKSSPP N ETTQ+ +DN+S+L  PD
Sbjct: 299 LPSNGHPTLEAIGPLIQTHMEQKKNQFDHSVTPDNKSSPPLNTETTQSSKDNVSDLTHPD 358

Query: 241 GEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAET 300
           GEA+GPKRRMLGPEMPSAELLAAAAKLTEAQTELRE ELEED+ELFVGPPPPAMVAEAET
Sbjct: 359 GEATGPKRRMLGPEMPSAELLAAAAKLTEAQTELREVELEEDHELFVGPPPPAMVAEAET 418

Query: 301 ANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA 360
           ANEAERFEEV+RIMDVEGDSPYDI GLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA
Sbjct: 419 ANEAERFEEVARIMDVEGDSPYDIFGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA 478

Query: 361 FIRLNNAFKELQDPDKRKALDDKIKLKEEQEQFKVELRGMREAAKWRQSQGISMDGDEEL 420
           FIRLNNAFKELQDPDKRKALDDKIK KEEQEQFKVELRGMREAAKWR+SQGISM+GD+EL
Sbjct: 479 FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDDEL 538

Query: 421 LANTEDDVKVEPKRDEWMTTLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPLDRAQK 480
           LAN +D+VKVEPKRDEWMT+LPPERKPGMTMQSSRFSK SKEGRGDTSVWTDTP DRAQK
Sbjct: 539 LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKGSKEGRGDTSVWTDTPSDRAQK 598

Query: 481 AKTSYLEAYNEATALASNEEAKKHSSESALVDKYNKEKRSKSLIEKHREETRSRA-KKKS 540
           AKTSYLEAYNEA ALASNEEAKK+SSESALVDKYN+EKRSK LIEKHRE  RS   KKKS
Sbjct: 599 AKTSYLEAYNEAAALASNEEAKKNSSESALVDKYNREKRSKPLIEKHREGARSSGGKKKS 658

Query: 541 KQ-VAEKGDDWVGKHPWKPWDREKDLTAGRKTVNFDPENMAQGLGSRFSSGSYQRNFL 581
           KQ V EK ++WVGKHPWKPWDREKDLTAGRKT+NFDPENMAQGLGSRFSSGSYQRNFL
Sbjct: 659 KQQVGEKEEEWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQRNFL 716

BLAST of MC11g1226 vs. NCBI nr
Match: XP_023521008.1 (DNA ligase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 910 bits (2352), Expect = 0.0
Identity = 503/598 (84.11%), Postives = 538/598 (89.97%), Query Frame = 0

Query: 1   MGSGRKKWRTDDSEPSSSSPSSDDDSDTSASRSRRHHRGGGSRREKDKKRESR------K 60
           MGSGRKK RT+ SEPSSSSPSS D+SD+S+ RSRRH R G SRREKDKKRESR      K
Sbjct: 1   MGSGRKKKRTEYSEPSSSSPSSSDNSDSSSMRSRRHRRSGSSRREKDKKRESRIRDRERK 60

Query: 61  RKDRMRERDRKRRKKKSRREGKRKRD-YFSESD-----DGSSGESELEKVR---IEPEVV 120
           RKDR RERDRK RKKK RRE KRKRD Y SESD      G+S +SE E+ +   IEPEVV
Sbjct: 61  RKDRNRERDRKMRKKKLRREAKRKRDDYGSESDAESSRSGNSSDSEFERRKLRSIEPEVV 120

Query: 121 VRQLLKEFPDVGDDLEQLLKMIDDGQAVDISGISERSLTKRLKKLFRSLNLEETQDRVFL 180
           VR+LLKEFPDVG+DL+QLL MIDDGQAVDISGISER+L KRLKKLF SLNLEE+Q+RVFL
Sbjct: 121 VRELLKEFPDVGNDLKQLLSMIDDGQAVDISGISERTLAKRLKKLFLSLNLEESQERVFL 180

Query: 181 LPSNGRSTLELIGPIIQFHMEQKRNQFDHSVTLDNKSSPPSNMETTQAREDNMSELACPD 240
           LPSNG  TLE IGP+IQ HMEQK+NQFDHSVT DNKSSPP N ETTQ+ ++N+S+L  PD
Sbjct: 181 LPSNGHPTLEAIGPLIQTHMEQKKNQFDHSVTPDNKSSPPLNTETTQSSKENVSDLTHPD 240

Query: 241 GEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAET 300
           GEA+GPKRRMLGPEMPSAELLAAAAKLTEAQTELRE ELEED+ELFVGPPPPAMVAEAET
Sbjct: 241 GEATGPKRRMLGPEMPSAELLAAAAKLTEAQTELREVELEEDHELFVGPPPPAMVAEAET 300

Query: 301 ANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA 360
           ANEAERFEEV+RIMDVEGDSPYDI GLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA
Sbjct: 301 ANEAERFEEVARIMDVEGDSPYDIFGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA 360

Query: 361 FIRLNNAFKELQDPDKRKALDDKIKLKEEQEQFKVELRGMREAAKWRQSQGISMDGDEEL 420
           FIRLNNAFKELQDPDKRKALDDKIK KEEQEQFKVELRGMREAAKWR+SQGISM+GD+EL
Sbjct: 361 FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDDEL 420

Query: 421 LANTEDDVKVEPKRDEWMTTLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPLDRAQK 480
           LAN +D+VKVEPKRDEWMT+LPPERKPGMTMQSSRFSK SKEGRGDTSVWTDTP DRAQK
Sbjct: 421 LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKGSKEGRGDTSVWTDTPSDRAQK 480

Query: 481 AKTSYLEAYNEATALASNEEAKKHSSESALVDKYNKEKRSKSLIEKHREETRSRA-KKKS 540
           AKTSYLEAYNEA ALASNEEAKK+SSESALVDKYN+EKRSK LIEKHRE  RS   KKKS
Sbjct: 481 AKTSYLEAYNEAAALASNEEAKKNSSESALVDKYNREKRSKPLIEKHREGARSSGGKKKS 540

Query: 541 KQ-VAEKGDDWVGKHPWKPWDREKDLTAGRKTVNFDPENMAQGLGSRFSSGSYQRNFL 581
           KQ V EK ++WVGKHPWKPWDREKDLTAGRKT+NFDPENMAQGLGSRFSSGSYQRNFL
Sbjct: 541 KQQVGEKEEEWVGKHPWKPWDREKDLTAGRKTINFDPENMAQGLGSRFSSGSYQRNFL 598

BLAST of MC11g1226 vs. ExPASy TrEMBL
Match: A0A6J1DVD5 (uncharacterized protein LOC111023459 OS=Momordica charantia OX=3673 GN=LOC111023459 PE=4 SV=1)

HSP 1 Score: 1091 bits (2821), Expect = 0.0
Identity = 580/581 (99.83%), Postives = 581/581 (100.00%), Query Frame = 0

Query: 1   MGSGRKKWRTDDSEPSSSSPSSDDDSDTSASRSRRHHRGGGSRREKDKKRESRKRKDRMR 60
           MGSGRKKWRTDDSEPSSSSPSSDDDSDTSASRSRRHHRGGGSRREKDKKRESRKRKDRMR
Sbjct: 1   MGSGRKKWRTDDSEPSSSSPSSDDDSDTSASRSRRHHRGGGSRREKDKKRESRKRKDRMR 60

Query: 61  ERDRKRRKKKSRREGKRKRDYFSESDDGSSGESELEKVRIEPEVVVRQLLKEFPDVGDDL 120
           ERDRKRRKKKSRREGKRKRDYFSESDDGSSGESELEKVRIEPEVVVRQLLKEFPDVGDDL
Sbjct: 61  ERDRKRRKKKSRREGKRKRDYFSESDDGSSGESELEKVRIEPEVVVRQLLKEFPDVGDDL 120

Query: 121 EQLLKMIDDGQAVDISGISERSLTKRLKKLFRSLNLEETQDRVFLLPSNGRSTLELIGPI 180
           EQLLKMIDDGQAVDISGISERSLTKRLKKLFRSLNLEETQDRVFLLPSNGRSTLELIGPI
Sbjct: 121 EQLLKMIDDGQAVDISGISERSLTKRLKKLFRSLNLEETQDRVFLLPSNGRSTLELIGPI 180

Query: 181 IQFHMEQKRNQFDHSVTLDNKSSPPSNMETTQAREDNMSELACPDGEASGPKRRMLGPEM 240
           IQFHMEQKRNQFDHSVTLDNKSSPPSNMETTQAREDNMSELACPDGEASGPKRRMLGPEM
Sbjct: 181 IQFHMEQKRNQFDHSVTLDNKSSPPSNMETTQAREDNMSELACPDGEASGPKRRMLGPEM 240

Query: 241 PSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAETANEAERFEEVSRIMD 300
           PSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAETANEAERFEEVSRIMD
Sbjct: 241 PSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAETANEAERFEEVSRIMD 300

Query: 301 VEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFIRLNNAFKELQDPD 360
           VEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFIRLNNAFKELQDPD
Sbjct: 301 VEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFIRLNNAFKELQDPD 360

Query: 361 KRKALDDKIKLKEEQEQFKVELRGMREAAKWRQSQGISMDGDEELLANTEDDVKVEPKRD 420
           KRKALDDKIKLKEEQEQFKVELRGMREAAKWRQSQGISMDGDEELLANTEDDVKVEPKRD
Sbjct: 361 KRKALDDKIKLKEEQEQFKVELRGMREAAKWRQSQGISMDGDEELLANTEDDVKVEPKRD 420

Query: 421 EWMTTLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPLDRAQKAKTSYLEAYNEATAL 480
           EWMTTLPPERKPG+TMQSSRFSKSSKEGRGDTSVWTDTPLDRAQKAKTSYLEAYNEATAL
Sbjct: 421 EWMTTLPPERKPGITMQSSRFSKSSKEGRGDTSVWTDTPLDRAQKAKTSYLEAYNEATAL 480

Query: 481 ASNEEAKKHSSESALVDKYNKEKRSKSLIEKHREETRSRAKKKSKQVAEKGDDWVGKHPW 540
           ASNEEAKKHSSESALVDKYNKEKRSKSLIEKHREETRSRAKKKSKQVAEKGDDWVGKHPW
Sbjct: 481 ASNEEAKKHSSESALVDKYNKEKRSKSLIEKHREETRSRAKKKSKQVAEKGDDWVGKHPW 540

Query: 541 KPWDREKDLTAGRKTVNFDPENMAQGLGSRFSSGSYQRNFL 581
           KPWDREKDLTAGRKTVNFDPENMAQGLGSRFSSGSYQRNFL
Sbjct: 541 KPWDREKDLTAGRKTVNFDPENMAQGLGSRFSSGSYQRNFL 581

BLAST of MC11g1226 vs. ExPASy TrEMBL
Match: A0A6J1EHJ8 (DNA ligase 1 OS=Cucurbita moschata OX=3662 GN=LOC111433443 PE=4 SV=1)

HSP 1 Score: 912 bits (2356), Expect = 0.0
Identity = 501/597 (83.92%), Postives = 537/597 (89.95%), Query Frame = 0

Query: 1   MGSGRKKWRTDDSEPSSSSPSSDDDSDTSASRSRRHHRGGGSRREKDKKRESR------K 60
           MGSGRKK RT+ SEPSSSSPSS D+SD+S+ RSRRH R G SRREKDKKRESR      K
Sbjct: 1   MGSGRKKKRTECSEPSSSSPSSSDNSDSSSMRSRRHRRSGSSRREKDKKRESRIRDRERK 60

Query: 61  RKDRMRERDRKRRKKKSRREGKRKRD-YFSESD-----DGSSGESELEKVR---IEPEVV 120
           RKDR RERDRK RKKK RRE KRKRD Y SESD      G+S +SE E+ +   +EPEVV
Sbjct: 61  RKDRNRERDRKMRKKKLRREAKRKRDDYGSESDAESSRSGNSSDSEFERRKLRSVEPEVV 120

Query: 121 VRQLLKEFPDVGDDLEQLLKMIDDGQAVDISGISERSLTKRLKKLFRSLNLEETQDRVFL 180
           VR+LLKEFPDVG+DL+QLL MIDDGQAVDISGISER+L KRLKKLF SLNLEE+Q+RVFL
Sbjct: 121 VRELLKEFPDVGNDLKQLLSMIDDGQAVDISGISERTLAKRLKKLFLSLNLEESQERVFL 180

Query: 181 LPSNGRSTLELIGPIIQFHMEQKRNQFDHSVTLDNKSSPPSNMETTQAREDNMSELACPD 240
           LPSNG  TLE IGP+IQ HMEQK+NQFDHSVT DNKSSPP N ETTQ+ +DN+S+L  PD
Sbjct: 181 LPSNGHPTLEAIGPLIQTHMEQKKNQFDHSVTPDNKSSPPLNTETTQSSKDNVSDLTHPD 240

Query: 241 GEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAET 300
           GEA+GPKRRMLGPEMPSAELLAAAAKLTEAQTELRE ELEED+ELFVGPPPPAMVAEAET
Sbjct: 241 GEATGPKRRMLGPEMPSAELLAAAAKLTEAQTELREVELEEDHELFVGPPPPAMVAEAET 300

Query: 301 ANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA 360
           ANEAERFEEV+RIMDVEGDSPYDI GLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA
Sbjct: 301 ANEAERFEEVARIMDVEGDSPYDIFGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQA 360

Query: 361 FIRLNNAFKELQDPDKRKALDDKIKLKEEQEQFKVELRGMREAAKWRQSQGISMDGDEEL 420
           FIRLNNAFKELQDPDKRKALDDKIK KEEQEQFKVELRGMREAAKWR+SQGISM+GD+EL
Sbjct: 361 FIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISMEGDDEL 420

Query: 421 LANTEDDVKVEPKRDEWMTTLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPLDRAQK 480
           LAN +D+VKVEPKRDEWMT+LPPERKPGMTMQSSRFSK SKEGRGDTSVWTDTP DRAQK
Sbjct: 421 LANMDDEVKVEPKRDEWMTSLPPERKPGMTMQSSRFSKGSKEGRGDTSVWTDTPSDRAQK 480

Query: 481 AKTSYLEAYNEATALASNEEAKKHSSESALVDKYNKEKRSKSLIEKHREETRSRAKKKSK 540
           AKTSYLEAYNEA ALAS+EEAKK+SSESALVDKYN+EKRSK LIEKHRE  RS  KKKSK
Sbjct: 481 AKTSYLEAYNEAAALASSEEAKKNSSESALVDKYNREKRSKPLIEKHREGARSSGKKKSK 540

Query: 541 Q-VAEKGDDWVGKHPWKPWDREKDLTAGRKTVNFDPENMAQGLGSRFSSGSYQRNFL 581
           Q V EK ++WVGKHPWKPWDREKDLTAGRKT+N DPENMAQGLGSRFSSGSYQRNFL
Sbjct: 541 QQVGEKEEEWVGKHPWKPWDREKDLTAGRKTINLDPENMAQGLGSRFSSGSYQRNFL 597

BLAST of MC11g1226 vs. ExPASy TrEMBL
Match: A0A1S3CFW3 (uncharacterized protein LOC103500427 OS=Cucumis melo OX=3656 GN=LOC103500427 PE=4 SV=1)

HSP 1 Score: 902 bits (2330), Expect = 0.0
Identity = 501/601 (83.36%), Postives = 540/601 (89.85%), Query Frame = 0

Query: 1   MGSGRKKWRTDDSE-PSSSSPSSDDDSDTSASRSRRHHRGGGSRREKDKKRESR------ 60
           MGS RKK RT  SE PSSSSPSS DDSD+S+ RSRRH+R   SRREK+K+RESR      
Sbjct: 51  MGSRRKKNRTVGSEDPSSSSPSSSDDSDSSSMRSRRHYRSDSSRREKEKERESRIRDRER 110

Query: 61  KRKDRMRERDR----KRRKKKSRREGKRKRDYF-SESDDGSSG-------ESELEKVRI- 120
           KRK+R RERDR    KRRKKK RRE KRKRD F S+SD+ SS        +S+  KV+I 
Sbjct: 111 KRKNRNRERDRERDRKRRKKKLRRESKRKRDDFGSDSDNESSHSGDSVDTKSDRRKVKIM 170

Query: 121 EPEVVVRQLLKEFPDVGDDLEQLLKMIDDGQAVDISGISERSLTKRLKKLFRSLNLEETQ 180
           EPEV+VRQLLK+FPD+G+DL+QLLKMIDDGQAVDISGIS +SLTKRLKKLF SLNLEE+Q
Sbjct: 171 EPEVIVRQLLKDFPDIGNDLKQLLKMIDDGQAVDISGISVKSLTKRLKKLFLSLNLEESQ 230

Query: 181 DRVFLLPSNGRSTLELIGPIIQFHMEQKRNQFDHSVTLDNKSSPPSNMETTQAREDNMSE 240
           +RVFLLPS+   TLE+IGP+I+ HM+QK N  D S+TLD+KSSPP NMETTQAREDNMSE
Sbjct: 231 ERVFLLPSSVHPTLEVIGPLIEMHMDQKSNPVDDSITLDDKSSPPLNMETTQAREDNMSE 290

Query: 241 LACPDGEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMV 300
           LA PDGEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEED+ELFVGPPPPAMV
Sbjct: 291 LAYPDGEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDDELFVGPPPPAMV 350

Query: 301 AEAETANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHP 360
           AEAETANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHP
Sbjct: 351 AEAETANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHP 410

Query: 361 QANQAFIRLNNAFKELQDPDKRKALDDKIKLKEEQEQFKVELRGMREAAKWRQSQGISMD 420
           QANQAFIRLNNAFKELQDPDKRKALDDKIK KEEQEQFKVELRGMREAAKWR+SQGISM+
Sbjct: 411 QANQAFIRLNNAFKELQDPDKRKALDDKIKFKEEQEQFKVELRGMREAAKWRKSQGISME 470

Query: 421 GDEELLANTEDDVKVEPKRDEWMTTLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPL 480
           GDEELLAN +D+V  EPKRDEWMTTLPPERKPGMTMQSSRFSKSSKEGRGDTS+WTDTP 
Sbjct: 471 GDEELLANMDDEVTAEPKRDEWMTTLPPERKPGMTMQSSRFSKSSKEGRGDTSIWTDTPS 530

Query: 481 DRAQKAKTSYLEAYNEATALASNEEAKKHSSESALVDKYNKEKRSKSLIEKHREETRSRA 540
           DRAQKAK SYLEAYNEA ALASNEEAKK+S+ESALVDKYN+EKRSKSL+EKHRE  +SR 
Sbjct: 531 DRAQKAKMSYLEAYNEAAALASNEEAKKYSAESALVDKYNQEKRSKSLVEKHREGVKSRG 590

Query: 541 KKKSKQVAEKGDDWVGKHPWKPWDREKDLTAGRKTVNFDPENMAQGLGSRFSSGSYQRNF 581
           K KSKQVAEK DDWVGKHPWKPWDREKDLTAGRK++NFDPENMAQGLGSRFSSGSYQRNF
Sbjct: 591 KNKSKQVAEKEDDWVGKHPWKPWDREKDLTAGRKSINFDPENMAQGLGSRFSSGSYQRNF 650

BLAST of MC11g1226 vs. ExPASy TrEMBL
Match: A0A2C9WFD9 (J domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_02G191800 PE=4 SV=1)

HSP 1 Score: 711 bits (1834), Expect = 3.81e-251
Identity = 407/598 (68.06%), Postives = 473/598 (79.10%), Query Frame = 0

Query: 1   MGSGRKKWRTDDSEPSSSSPSSDDDSDTSASRSRRHHR----GGGSRREKDKKRESR--- 60
           MGS  +K   +D+ P+SSS S DDDS+TS  R RR  +      GS R +D+KRE R   
Sbjct: 1   MGSKTRKKHREDT-PTSSSNSDDDDSETSEDRRRRRRKDRRSSDGSSRREDRKRERRDEK 60

Query: 61  --KRKDRMRERDRKRRKKKSRREGKRKRDYFSESDDGSSGES-------ELEKVRIEPEV 120
             KRK+R  ERDRKRRK       ++KRDY SES+ GS  ES       E ++ R++PE 
Sbjct: 61  RRKRKERK-ERDRKRRK------SRKKRDYDSESESGSGSESGSGSDAEEPKRQRVKPED 120

Query: 121 VVRQLLKEFPDVGDDLEQLLKMIDDGQAVDISGISERSLTKRLKKLFRSLNLEETQDRVF 180
           VV+++L EFPDVGDDL+QLL+MID GQAVDI GISE+ L K LKKLF SLNL E  DRVF
Sbjct: 121 VVKEMLMEFPDVGDDLKQLLQMIDSGQAVDIKGISEKPLIKHLKKLFVSLNLMEKGDRVF 180

Query: 181 LLPSNGRSTLELIGPIIQFHMEQKRNQFDHSVTLDNKSSPPSNMETTQAREDNMSELACP 240
           LLPSN   TLEL+GP+I+ H+  K  + D     D  S PP   E+ Q  ++N S +  P
Sbjct: 181 LLPSNNCPTLELVGPLIEAHIRSKEQKVD-----DVHSVPPV-AESKQLSDNNNSVMPAP 240

Query: 241 DGEASGPKRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAE 300
           D +ASGP+RRM+GPEMPSAELLAAAAKLTEA  ELREAELEED+ELF+GP PPAMVAEAE
Sbjct: 241 DDDASGPRRRMIGPEMPSAELLAAAAKLTEAHAELREAELEEDDELFIGPAPPAMVAEAE 300

Query: 301 TANEAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQ 360
           +ANEAERFEEV+RIM VEGDSPYD++G N N S ENIKKRYWK+SLLVHPDKCSHPQA+Q
Sbjct: 301 SANEAERFEEVTRIMGVEGDSPYDVVGANHNTSAENIKKRYWKLSLLVHPDKCSHPQAHQ 360

Query: 361 AFIRLNNAFKELQDPDKRKALDDKIKLKEEQEQFKVELRGMREAAKWRQSQGISMDGDEE 420
           AFI+LN AFKELQDPDKRK +D+KIKLKEEQEQFKVEL+ MREAA+WR+SQGISM+GD+E
Sbjct: 361 AFIKLNKAFKELQDPDKRKLMDEKIKLKEEQEQFKVELKAMREAAQWRRSQGISMEGDDE 420

Query: 421 LLANTEDDVKVEPKRDEWMTTLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPLDRAQ 480
           LLA TE  VKV PKRDEWMTTLPPERKPGMTM SS+FSK S+EGRGDTSVWTDTP DRAQ
Sbjct: 421 LLAETE--VKVAPKRDEWMTTLPPERKPGMTMHSSKFSKGSREGRGDTSVWTDTPSDRAQ 480

Query: 481 KAKTSYLEAYNEATALASNEEAKKHSSESA-LVDKYNKEKRSKSLIEKHREETRSRAKKK 540
           KAK +YLEAYNEA ALASNEE KK SS  A LVDKYNK KRSKSL++KH+EE  SR+KKK
Sbjct: 481 KAKMNYLEAYNEAAALASNEEEKKRSSADADLVDKYNKAKRSKSLVQKHQEEAASRSKKK 540

Query: 541 SKQVAEKGDDWVGKHPWKPWDREKDLTAGRKTVNFDPENMAQGLGSRFSSGSYQRNFL 581
           SK+  EK ++W G+HPWKPWDREKDL AGR+ + FD +NMAQGL SRFSSG++QRNFL
Sbjct: 541 SKEQPEK-EEWAGQHPWKPWDREKDLVAGRQNIKFDADNMAQGLSSRFSSGNFQRNFL 581

BLAST of MC11g1226 vs. ExPASy TrEMBL
Match: A0A0B0MMZ6 (DnaJ subfamily C member 14 OS=Gossypium arboreum OX=29729 GN=LOC108461091 PE=4 SV=1)

HSP 1 Score: 699 bits (1805), Expect = 1.11e-246
Identity = 391/593 (65.94%), Postives = 476/593 (80.27%), Query Frame = 0

Query: 1   MGSGRKKWRTDDSEPSSSSPSSDDDSDTSASRSRRHHR-----GGGSRREKDKKRESRKR 60
           MG   +K   DD+  SSSSPS+ DDS++S  R  RH +        SRREK++KRE RKR
Sbjct: 1   MGRKTRKKHVDDTPTSSSSPSTSDDSESSLERRHRHRKVRRRKDESSRREKERKREKRKR 60

Query: 61  KDRMRERDRKRRKKKSRREGKRKRDYFSESDDGSSG----ESELEKVRIEPEVVVRQLLK 120
           K++ RER R++ +++ RRE  ++RDY S+S  GS      +SE +K R+EPEVVV+Q+LK
Sbjct: 61  KEKERERKRRKSRREDRRE--KRRDYESDSKSGSGSSSGSDSEEDKRRVEPEVVVQQILK 120

Query: 121 EFPDVGDDLEQLLKMIDDGQAVDISGISERSLTKRLKKLFRSLNLEETQDRVFLLPSNGR 180
           EFP+VG+DL+QLL+MIDDGQAVDI GISERSL K LKKLF SL L+E  DRVFLL S  R
Sbjct: 121 EFPNVGNDLKQLLQMIDDGQAVDIKGISERSLNKHLKKLFLSLKLKENGDRVFLLSSKSR 180

Query: 181 STLELIGPIIQFHMEQKRNQFDHSVTLDNKSSPPSNMETTQAREDNMSELACPDGEASGP 240
            TL+++G +IQ H E K  Q  +S  + +  S P + E++Q  ++N S       +++ P
Sbjct: 181 PTLDVVGHLIQAHTEPKEQQRTNSGLVKDAHSIPEHAESSQVMDENNSYR----DDSATP 240

Query: 241 KRRMLGPEMPSAELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAETANEAER 300
           KRR++GP MPSAELLAAAAKLTEAQ ELREAE+EED+ELF+GPPPPA+VAEAE+ANEAER
Sbjct: 241 KRRVIGPAMPSAELLAAAAKLTEAQAELREAEIEEDDELFIGPPPPALVAEAESANEAER 300

Query: 301 FEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFIRLNN 360
           FEEV+RI+ VE D PYD++G NRN + +NIKK+YWK+SL+VHPDKC+HPQA+QAFI LN 
Sbjct: 301 FEEVTRIVGVESDCPYDVIGANRNTTADNIKKKYWKLSLMVHPDKCAHPQAHQAFIILNK 360

Query: 361 AFKELQDPDKRKALDDKIKLKEEQEQFKVELRGMREAAKWRQSQGISMDGDEELLANTED 420
           AFKELQDPDKRKALD+KIKLKEEQE+FK ELR MREAA+WR+ QGISM+GD+ELLA  E 
Sbjct: 361 AFKELQDPDKRKALDEKIKLKEEQEEFKAELRAMREAAQWRRLQGISMEGDDELLAEVE- 420

Query: 421 DVKVEPKRDEWMTTLPPERKPGMTMQSSRFSKSSKEGRGDTSVWTDTPLDRAQKAKTSYL 480
            VK  PKRDEWMTTLPPERKPG+TMQS++FSKS+KEGRGDTSVWTDTPL+RAQKAK  YL
Sbjct: 421 -VKAPPKRDEWMTTLPPERKPGVTMQSTKFSKSAKEGRGDTSVWTDTPLERAQKAKMHYL 480

Query: 481 EAYNEATALASNEE--AKKHSSESALVDKYNKEKRSKSLIEKHREETRSRAKKKSKQVAE 540
           EAYNEA ALASNEE   K+ SS++ LVDKYNKEKRSKSL++KH+EE   R KKKSKQ +E
Sbjct: 481 EAYNEAAALASNEEEKTKRSSSDADLVDKYNKEKRSKSLVQKHQEEASKRPKKKSKQQSE 540

Query: 541 KG-DDWVGKHPWKPWDREKDLTAGRKTVNFDPENMAQGLGSRFSSGSYQRNFL 581
           K  D+W GKHPWKPWDREKDLTAGR++VN D ENM +GL SRFS+GS+QRNFL
Sbjct: 541 KEKDEWEGKHPWKPWDREKDLTAGRQSVNLDTENMVKGLTSRFSTGSFQRNFL 585

BLAST of MC11g1226 vs. TAIR 10
Match: AT1G65280.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 592.0 bits (1525), Expect = 5.1e-169
Identity = 349/588 (59.35%), Postives = 446/588 (75.85%), Query Frame = 0

Query: 10  TDDSEPSSSSPSSDDDSDTSA------SRSRRHHRGGGSRREKDKKRESRKRKDRMRERD 69
           T  S  SSSS  S D+SD+S+       + RR   GG   REK +KRE  +++ ++  ++
Sbjct: 15  TPSSATSSSSSYSSDESDSSSRKWRKKQKERRKSDGGSYEREKRRKREKERKRKKIERKE 74

Query: 70  RKRRKKKSRREGKRKRDYFSESDDGSSGESELEKVRIEPEVVVRQLLKEFPDVGDDLEQL 129
           RKRR  K +++ K KR+Y S++ +  SG         +PE VV+++L EFP+VG+DL+QL
Sbjct: 75  RKRRDMKKKKKTK-KREYESDT-ESYSGSDSFSDQEDDPETVVKEMLLEFPNVGNDLKQL 134

Query: 130 LKMIDDGQAVDISGISERSLTKRLKKLFRSLNLEETQDRVFLLPSNGRSTLELIGPIIQF 189
           LKMIDDGQAVDI GISE +L KRLKKLF SL L+E  DRVFLLP     +L+++G +I  
Sbjct: 135 LKMIDDGQAVDIKGISESTLKKRLKKLFLSLKLKERGDRVFLLPPGASPSLDVVGHLI-- 194

Query: 190 HMEQKRNQFDHSVTLDNKSSPPSNMETTQAREDNMSELACPDGEASGPKRRMLGPEMPSA 249
               K  + D   +LD+ SSP    E   A E+ +   +  D   +GPK+R++GP MPSA
Sbjct: 195 ----KGGEEDVEKSLDD-SSPLKETEKGLADENGLGSNSADD--VTGPKKRVIGPAMPSA 254

Query: 250 ELLAAAAKLTEAQTELREAELEEDNELFVGPPPPAMVAEAETANEAERFEEVSRIMDVEG 309
           ELLAAAAKLTEAQ ELREAELEED+E F+GP PPA+VAE  ++NEAERFEEV+RIM+ + 
Sbjct: 255 ELLAAAAKLTEAQAELREAELEEDSEYFIGPAPPAVVAEVASSNEAERFEEVTRIMEADA 314

Query: 310 DSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFIRLNNAFKELQDPDKRK 369
           +SPYD+LG+N N++ +N+KKRYWK+SLLVHPDKCSHPQA +AF+ LN AFKELQDP+KRK
Sbjct: 315 NSPYDVLGVNHNMAADNMKKRYWKLSLLVHPDKCSHPQAQEAFVLLNKAFKELQDPEKRK 374

Query: 370 ALDDKIKLKEEQEQFKVELRGMREAAKWRQSQGISMDGDEELLANTEDDVKVEPKRDEWM 429
           A+DDKIKLKEEQE FKVELR M+EAA+WR+SQGISM+GD ELLA TE  VK EPKRDEWM
Sbjct: 375 AMDDKIKLKEEQEAFKVELRSMQEAAQWRRSQGISMEGDAELLAATE--VKPEPKRDEWM 434

Query: 430 TTLPPERKPGMTM-QSSRFSKSSKEGRGDTSVWTDTPLDRAQKAKTSYLEAYNEATALAS 489
           TTLPPERK G+ + QS+ FS++++EGRGDT+ WTDTP+D+A++AK +YLEAYN+A+ALAS
Sbjct: 435 TTLPPERKVGVAVQQSTTFSRNAREGRGDTTAWTDTPMDKAERAKMNYLEAYNKASALAS 494

Query: 490 NE-EAKKHSSESALVDKYNKEKRSKSLIEKHREE---TRSRAKKK-----SKQVAEKGDD 549
           NE E  K S ++ LVDKYNKEKR+KSL+EKHRE+   + SR KKK     SK+  EK D+
Sbjct: 495 NEGENMKRSMDAELVDKYNKEKRAKSLVEKHREDSSSSSSRLKKKKKLSSSKEKTEK-DE 554

Query: 550 WVGKHPWKPWDREKDLTAGRKTVNFDPENMAQGLGSRFSSGSYQRNFL 582
           WVGKHPWKPWDRE DLTAGR+ V  D + MA+GL S+FSSG++QR+FL
Sbjct: 555 WVGKHPWKPWDRENDLTAGRQNVKLDADGMAEGLASKFSSGNFQRSFL 588

BLAST of MC11g1226 vs. TAIR 10
Match: AT5G22080.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 6.9e-09
Identity = 39/131 (29.77%), Postives = 74/131 (56.49%), Query Frame = 0

Query: 288 EAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFI 347
           E ER  EV RI+     +P++ L L+ + S +++K++Y K+SL+VHPDKC HPQA +AF 
Sbjct: 21  EVERDNEVGRILSCFKLNPFEHLNLSFDSSTDDVKRQYRKISLMVHPDKCKHPQAQEAFG 80

Query: 348 RLNNAFKELQDPDKRKALDDKIKLKEEQEQFKVELRGMREAAKWRQSQGISMDGDEELLA 407
            L  A + L +  +R  +  ++   +E+ + K + +  ++ A   + + +  +G  E + 
Sbjct: 81  ALAKAQQLLLNDQERDYILTQVHAAKEELKMKRKKQLKKDTAS--KIKSLVDEGKHEHIY 140

Query: 408 NTEDDVKVEPK 419
              ++ + E K
Sbjct: 141 EQSEEFQKELK 149

BLAST of MC11g1226 vs. TAIR 10
Match: AT5G22080.2 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 6.9e-09
Identity = 39/131 (29.77%), Postives = 74/131 (56.49%), Query Frame = 0

Query: 288 EAERFEEVSRIMDVEGDSPYDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFI 347
           E ER  EV RI+     +P++ L L+ + S +++K++Y K+SL+VHPDKC HPQA +AF 
Sbjct: 21  EVERDNEVGRILSCFKLNPFEHLNLSFDSSTDDVKRQYRKISLMVHPDKCKHPQAQEAFG 80

Query: 348 RLNNAFKELQDPDKRKALDDKIKLKEEQEQFKVELRGMREAAKWRQSQGISMDGDEELLA 407
            L  A + L +  +R  +  ++   +E+ + K + +  ++ A   + + +  +G  E + 
Sbjct: 81  ALAKAQQLLLNDQERDYILTQVHAAKEELKMKRKKQLKKDTAS--KIKSLVDEGKHEHIY 140

Query: 408 NTEDDVKVEPK 419
              ++ + E K
Sbjct: 141 EQSEEFQKELK 149

BLAST of MC11g1226 vs. TAIR 10
Match: AT3G57340.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 56.6 bits (135), Expect = 7.7e-08
Identity = 28/75 (37.33%), Postives = 47/75 (62.67%), Query Frame = 0

Query: 293 EEVSRIMDVEGDSP-YDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFIRLNN 352
           E++S +  ++     Y+ILGL  N S+++++K Y K+SL VHPDK   P + +AF  ++ 
Sbjct: 100 EQISIVRKIKSKKDYYEILGLESNCSVDDVRKAYRKLSLKVHPDKNQAPGSEEAFKSVSK 159

Query: 353 AFKELQDPDKRKALD 367
           AF+ L + + RK  D
Sbjct: 160 AFQCLSNDEARKKYD 174

BLAST of MC11g1226 vs. TAIR 10
Match: AT3G57340.2 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 56.6 bits (135), Expect = 7.7e-08
Identity = 28/75 (37.33%), Postives = 47/75 (62.67%), Query Frame = 0

Query: 293 EEVSRIMDVEGDSP-YDILGLNRNLSIENIKKRYWKMSLLVHPDKCSHPQANQAFIRLNN 352
           E++S +  ++     Y+ILGL  N S+++++K Y K+SL VHPDK   P + +AF  ++ 
Sbjct: 100 EQISIVRKIKSKKDYYEILGLESNCSVDDVRKAYRKLSLKVHPDKNQAPGSEEAFKSVSK 159

Query: 353 AFKELQDPDKRKALD 367
           AF+ L + + RK  D
Sbjct: 160 AFQCLSNDEARKKYD 174