MC11g1216 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g1216
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	subtilisin-like protease SBT3.11
Location	MC11: 12048578 .. 12050159 (+)
RNA-Seq Expression	MC11g1216
Synteny	MC11g1216

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTATGTCTTGCTTTGCTGTTCTCAATTTCTCTGAATTTTCTTCTTAGCTCTGCAGCAATGTCCGGTCAGTACGCTTCTCCGGCGGTGCATATCGTTTACACGGAGAAGCCTCGCGATGAGGAACCTGAGGCCTATCACATCCGAACCCTAGTTTCTGTTCTCGGCAGGTTTGTGTTCTCTTCTCTTCCTTCTCTGTGTCCTTTCGCCGGAAAACGGTGGAAATTGGCGATGAATTTTGGTATAATTTGTTGATTTGCAGTGAAGAGGCTGCTAAAGAGGCTCTTGTGTATAGCTACAAGAATGCAGCCAGTGGATTCTCAGCTCGGCTTACTCCCGATCAGGTCGCGGAGGTTTCCAGTAAGTCGCGCTCTTAACATCAATCGTTAAGAATTCAGTATTTCTTGATCAATTGTTCTGTGAAATGCTGTCTTATCTCATGTATTTGATGAACTTCTGTAAGAAATTGATGCATCTGAGCTAGGGATATAAAAATTCGGTTCCTCGTATCAATCTAATGCAGAGTCATGTGGAACAGCTGATGATTTTTTGAGCTTATTGTGATGTGATCTGTACTTTTTTTCCAATTGTCTTGAGCCACTTATGTTGCTTGGTTTGGTGTGAAACAAAAGTTCTTACTCGTTGTGCATGTTCTTGAAGCTGTTTATTGATTGGTCAATGAGTTGGATCAGGATTTTGATTGCCCTTTATACTGTCATATGTTTTCTTCCTCCTTGATTCTTGTTGATCTAGCAATCTCAAGCTTGCTTTGTCCACACTTTGACTACATGCCTTTTTCTTTTTTCCTTTTCCAAATATTTGGGGTATCAAGAATTGGAGAATTGAGTGCTAAACAGTAAACATTATCTTCTTTTAATTTGACATGGCTGAATAAGAGAATCAAGTGATTAATGATAAGCTTTAATCTCAAACATTCTTGAGGTCATGGAACCGGATCGTGGCGACGGTTTATTTGAAAAATCAATATCCTACCAATTTTTCAAAGTTGTAAGATCATGTAGTTATTCCGGTGATCAATTGAGACATGTTGCTTGAGCTTAAATGCTCGTTTTTTCTTCTTCTTTCTTGAGTACAACAAATGTGCGGGTAAGGATTTGAACCTCTGACCTCAATGGAAGAATCAATTACACCTTATTCTTCAGCCTTTATCCAATCATTTCCAACCTATGGATCAGACACCACAACACCTTCGAAACTTGGATGTTTTGATATTACAGCAAAAATTATATCTTTAGCAAGTGTGTCATATGCTGCATTTTCTTTTTTGTCGAATCTCTAGTCTTGATAATGTGTCTTTATATTCGTAAGTTTTCTTTTTCTTTCTAATATGAGGTAGGAGATCGAGCATTCAACCTCAATGAAAGTAATAGATGTCTTATCAACTGAGTTATGTTCGGATTGAGATATTATGGTGTTATAAACCTTCAGAATCAGTATAAGATAGTGTTTCTTACGGAACTTTTTGAATTTTGGGACTTTGGCAGAACAACCAGGAGTTTTACATGTTGTCCAAAGCGGTACTCATCAGCTGCATTCAGGAGGAGGAGGAGTATCAAGGCTTCAC

mRNA sequence

CTATGTCTTGCTTTGCTGTTCTCAATTTCTCTGAATTTTCTTCTTAGCTCTGCAGCAATGTCCGGTCAGTACGCTTCTCCGGCGGTGCATATCGTTTACACGGAGAAGCCTCGCGATGAGGAACCTGAGGCCTATCACATCCGAACCCTAGTTTCTGTTCTCGGCAGTGAAGAGGCTGCTAAAGAGGCTCTTGTGTATAGCTACAAGAATGCAGCCAGTGGATTCTCAGCTCGGCTTACTCCCGATCAGGTCGCGGAGGTTTCCAAACAACCAGGAGTTTTACATGTTGTCCAAAGCGGTACTCATCAGCTGCATTCAGGAGGAGGAGGAGTATCAAGGCTTCAC

Coding sequence (CDS)

CTATGTCTTGCTTTGCTGTTCTCAATTTCTCTGAATTTTCTTCTTAGCTCTGCAGCAATGTCCGGTCAGTACGCTTCTCCGGCGGTGCATATCGTTTACACGGAGAAGCCTCGCGATGAGGAACCTGAGGCCTATCACATCCGAACCCTAGTTTCTGTTCTCGGCAGTGAAGAGGCTGCTAAAGAGGCTCTTGTGTATAGCTACAAGAATGCAGCCAGTGGATTCTCAGCTCGGCTTACTCCCGATCAGGTCGCGGAGGTTTCCAAACAACCAGGAGTTTTACATGTTGTCCAAAGCGGTACTCATCAGCTGCATTCAGGAGGAGGAGGAGTATCAAGGCTTCAC

Protein sequence

LCLALLFSISLNFLLSSAAMSGQYASPAVHIVYTEKPRDEEPEAYHIRTLVSVLGSEEAAKEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQLHSGGGGVSRLH

Homology

[Close]

BLAST of MC11g1216 vs. ExPASy Swiss-Prot
Match: Q1PDX5 (Subtilisin-like protease SBT3.11 OS=Arabidopsis thaliana OX=3702 GN=SBT3.11 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 3.5e-10
Identity = 34/88 (38.64%), Postives = 54/88 (61.36%), Query Frame = 0


Query: 22  GQYASPAVHIVYTEKPRDEEPE---AYHIRTLVSVLGSEEAAKEALVYSYKNAASGFSAR 81
           G Y    VHIVY  +    +PE   + H+R L S+LGS++ A E++V+SY+N  SGF+A 
Sbjct: 30  GAYEETKVHIVYLGEKEHNDPELVTSSHLRMLESLLGSKKDASESIVHSYRNGFSGFAAH 89

Query: 82  LTPDQVAEVSKQPGVLHVVQSGTHQLHS 107
           LT  Q  ++S+ P V+ V  +  ++L +
Sbjct: 90  LTDSQAEQISEHPDVVQVTPNTFYELQT 117

[Close]

BLAST of MC11g1216 vs. ExPASy Swiss-Prot
Match: Q9MAP5 (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.3e-09
Identity = 38/103 (36.89%), Postives = 63/103 (61.17%), Query Frame = 0


Query: 5   LLFSISLNFLLSSAAMSGQYASPAVHIVYTEKPRDEEPE---AYHIRTLVSVLGSEEAAK 64
           LL  +SL  +L++     +  S  VHIVY  + +  +PE     H + L S+LGS++ A 
Sbjct: 9   LLVLLSLITVLNATRARSETES-KVHIVYLGEKKHHDPEFVTESHHQMLASLLGSKKDAD 68

Query: 65  EALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQL 105
           +++VYSY++  SGF+A+LT  Q  +++  P V+HV+  G H+L
Sbjct: 69  DSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHEL 110

[Close]

BLAST of MC11g1216 vs. ExPASy Swiss-Prot
Match: Q9SZY3 (Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 8.7e-09
Identity = 42/107 (39.25%), Postives = 64/107 (59.81%), Query Frame = 0


Query: 2   CLALLF-SISLNFLLSSAAMSGQYASPAVHIVYTEKPRDEEPE---AYHIRTLVSVLGSE 61
           C  L+F +I LN L +  A +G  A   VHIVY  + + ++PE     H R L S+LGS+
Sbjct: 4   CRTLIFVAIILNGLSTFVAHAG--AESKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSK 63

Query: 62  EAAKEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQL 105
           E A  ++V+SY++  SGF+A+LT  Q  +++  P V+HV     +QL
Sbjct: 64  EDAHSSMVHSYRHGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQL 108

[Close]

BLAST of MC11g1216 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.1e-08
Identity = 37/107 (34.58%), Postives = 63/107 (58.88%), Query Frame = 0


Query: 1   LCLALLFSISLNFLLSSAAMSGQYASPAVHIVYTEKPRDEEPE---AYHIRTLVSVLGSE 60
           L L +  ++  +F+  S+      A   VHIVY  + + ++PE     H R L S+LGS+
Sbjct: 12  LSLVIFLNVQRSFVAESS------AKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSK 71

Query: 61  EAAKEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQL 105
           E A +++VYSY++  SGF+A+LT  Q  +++  P V+HV+    ++L
Sbjct: 72  EDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKL 112

[Close]

BLAST of MC11g1216 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.5e-08
Identity = 37/104 (35.58%), Postives = 64/104 (61.54%), Query Frame = 0


Query: 4   ALLFSISLNFLLSSAAMSGQYASPAVHIVYTEKPRDEEPE---AYHIRTLVSVLGSEEAA 63
           ++L  +SL  +L+ A  S   A   VHIVY  + + ++P+     H + L S+LGS++ A
Sbjct: 7   SVLVVLSLIIVLNVARAS---AKSKVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDA 66

Query: 64  KEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQL 105
            E++VYSY++  SGF+A+LT  Q  +++  P V+HV+    ++L
Sbjct: 67  HESMVYSYRHGFSGFAAKLTKSQAKKIADSPEVIHVIPDSYYEL 107

[Close]

BLAST of MC11g1216 vs. NCBI nr
Match: XP_022143620.1 (subtilisin-like protease SBT3.11 isoform X1 [Momordica charantia])

HSP 1 Score: 219 bits (558), Expect = 1.41e-71
Identity = 115/115 (100.00%), Postives = 115/115 (100.00%), Query Frame = 0


Query: 1   LCLALLFSISLNFLLSSAAMSGQYASPAVHIVYTEKPRDEEPEAYHIRTLVSVLGSEEAA 60
           LCLALLFSISLNFLLSSAAMSGQYASPAVHIVYTEKPRDEEPEAYHIRTLVSVLGSEEAA
Sbjct: 11  LCLALLFSISLNFLLSSAAMSGQYASPAVHIVYTEKPRDEEPEAYHIRTLVSVLGSEEAA 70

Query: 61  KEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQLHSGGGGVSRLH 115
           KEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQLHSGGGGVSRLH
Sbjct: 71  KEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQLHSGGGGVSRLH 125

[Close]

BLAST of MC11g1216 vs. NCBI nr
Match: XP_022143621.1 (subtilisin-like protease SBT3.11 isoform X2 [Momordica charantia] >XP_022143622.1 subtilisin-like protease SBT3.11 isoform X2 [Momordica charantia])

HSP 1 Score: 190 bits (482), Expect = 2.18e-60
Identity = 96/96 (100.00%), Postives = 96/96 (100.00%), Query Frame = 0


Query: 20  MSGQYASPAVHIVYTEKPRDEEPEAYHIRTLVSVLGSEEAAKEALVYSYKNAASGFSARL 79
           MSGQYASPAVHIVYTEKPRDEEPEAYHIRTLVSVLGSEEAAKEALVYSYKNAASGFSARL
Sbjct: 1   MSGQYASPAVHIVYTEKPRDEEPEAYHIRTLVSVLGSEEAAKEALVYSYKNAASGFSARL 60

Query: 80  TPDQVAEVSKQPGVLHVVQSGTHQLHSGGGGVSRLH 115
           TPDQVAEVSKQPGVLHVVQSGTHQLHSGGGGVSRLH
Sbjct: 61  TPDQVAEVSKQPGVLHVVQSGTHQLHSGGGGVSRLH 96

[Close]

BLAST of MC11g1216 vs. NCBI nr
Match: XP_004139693.1 (subtilisin-like protease SBT3.6 isoform X1 [Cucumis sativus])

HSP 1 Score: 177 bits (450), Expect = 4.69e-55
Identity = 96/122 (78.69%), Postives = 104/122 (85.25%), Query Frame = 0


Query: 3   LALLFSISLNFLLSSAAMSGQYAS---------PAVHIVYTEKPRDEEPEAYHIRTLVSV 62
           L  LFSISLNF LSS AMS Q AS         PAVHIVYTE+P +EEPEAYHIRTLVSV
Sbjct: 8   LLFLFSISLNFHLSSTAMSSQSASIPLVSSFDSPAVHIVYTERPHNEEPEAYHIRTLVSV 67

Query: 63  LGSEEAAKEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQLHSGGGGVSR 115
           LGSEEAA+EALVYSYKNAASGFSARLTPDQV E++KQPGVLHVVQSG ++LHSGGGGV+R
Sbjct: 68  LGSEEAAREALVYSYKNAASGFSARLTPDQVTEITKQPGVLHVVQSGNNKLHSGGGGVAR 127

[Close]

BLAST of MC11g1216 vs. NCBI nr
Match: KAG7025065.1 (Subtilisin-like protease SBT3.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 176 bits (447), Expect = 1.63e-54
Identity = 93/122 (76.23%), Postives = 106/122 (86.89%), Query Frame = 0


Query: 3   LALLFSISLNFLLSSAAMSGQ---------YASPAVHIVYTEKPRDEEPEAYHIRTLVSV 62
           L LLFSISLN  LSSA MS Q         +ASPAVHIVYTE+P +EEPEAYHIRTL SV
Sbjct: 14  LVLLFSISLNLFLSSAVMSDQSASIPVVSNFASPAVHIVYTERPHNEEPEAYHIRTLASV 73

Query: 63  LGSEEAAKEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQLHSGGGGVSR 115
           LGSEEAA+EALVYSYKNAASGFSARLTP+QVA+++KQPGVLHVVQSG+++LH+GGGGV+R
Sbjct: 74  LGSEEAAREALVYSYKNAASGFSARLTPEQVADITKQPGVLHVVQSGSNKLHTGGGGVAR 133

[Close]

BLAST of MC11g1216 vs. NCBI nr
Match: XP_022935862.1 (subtilisin-like protease SBT3.13 isoform X1 [Cucurbita moschata])

HSP 1 Score: 176 bits (447), Expect = 1.91e-54
Identity = 93/122 (76.23%), Postives = 106/122 (86.89%), Query Frame = 0


Query: 3   LALLFSISLNFLLSSAAMSGQ---------YASPAVHIVYTEKPRDEEPEAYHIRTLVSV 62
           L LLFSISLN  LSSA MS Q         +ASPAVHIVYTE+P +EEPEAYHIRTL SV
Sbjct: 19  LVLLFSISLNLFLSSAVMSDQSASIPVVSNFASPAVHIVYTERPHNEEPEAYHIRTLASV 78

Query: 63  LGSEEAAKEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQLHSGGGGVSR 115
           LGSEEAA+EALVYSYKNAASGFSARLTP+QVA+++KQPGVLHVVQSG+++LH+GGGGV+R
Sbjct: 79  LGSEEAAREALVYSYKNAASGFSARLTPEQVADITKQPGVLHVVQSGSNKLHTGGGGVAR 138

[Close]

BLAST of MC11g1216 vs. ExPASy TrEMBL
Match: A0A6J1CPA2 (subtilisin-like protease SBT3.11 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013481 PE=4 SV=1)

HSP 1 Score: 219 bits (558), Expect = 6.84e-72
Identity = 115/115 (100.00%), Postives = 115/115 (100.00%), Query Frame = 0


Query: 1   LCLALLFSISLNFLLSSAAMSGQYASPAVHIVYTEKPRDEEPEAYHIRTLVSVLGSEEAA 60
           LCLALLFSISLNFLLSSAAMSGQYASPAVHIVYTEKPRDEEPEAYHIRTLVSVLGSEEAA
Sbjct: 11  LCLALLFSISLNFLLSSAAMSGQYASPAVHIVYTEKPRDEEPEAYHIRTLVSVLGSEEAA 70

Query: 61  KEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQLHSGGGGVSRLH 115
           KEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQLHSGGGGVSRLH
Sbjct: 71  KEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQLHSGGGGVSRLH 125

[Close]

BLAST of MC11g1216 vs. ExPASy TrEMBL
Match: A0A6J1CQU0 (subtilisin-like protease SBT3.11 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111013481 PE=4 SV=1)

HSP 1 Score: 190 bits (482), Expect = 1.05e-60
Identity = 96/96 (100.00%), Postives = 96/96 (100.00%), Query Frame = 0


Query: 20  MSGQYASPAVHIVYTEKPRDEEPEAYHIRTLVSVLGSEEAAKEALVYSYKNAASGFSARL 79
           MSGQYASPAVHIVYTEKPRDEEPEAYHIRTLVSVLGSEEAAKEALVYSYKNAASGFSARL
Sbjct: 1   MSGQYASPAVHIVYTEKPRDEEPEAYHIRTLVSVLGSEEAAKEALVYSYKNAASGFSARL 60

Query: 80  TPDQVAEVSKQPGVLHVVQSGTHQLHSGGGGVSRLH 115
           TPDQVAEVSKQPGVLHVVQSGTHQLHSGGGGVSRLH
Sbjct: 61  TPDQVAEVSKQPGVLHVVQSGTHQLHSGGGGVSRLH 96

[Close]

BLAST of MC11g1216 vs. ExPASy TrEMBL
Match: A0A0A0K6B0 (Inhibitor I9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G291690 PE=4 SV=1)

HSP 1 Score: 177 bits (450), Expect = 2.27e-55
Identity = 96/122 (78.69%), Postives = 104/122 (85.25%), Query Frame = 0


Query: 3   LALLFSISLNFLLSSAAMSGQYAS---------PAVHIVYTEKPRDEEPEAYHIRTLVSV 62
           L  LFSISLNF LSS AMS Q AS         PAVHIVYTE+P +EEPEAYHIRTLVSV
Sbjct: 8   LLFLFSISLNFHLSSTAMSSQSASIPLVSSFDSPAVHIVYTERPHNEEPEAYHIRTLVSV 67

Query: 63  LGSEEAAKEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQLHSGGGGVSR 115
           LGSEEAA+EALVYSYKNAASGFSARLTPDQV E++KQPGVLHVVQSG ++LHSGGGGV+R
Sbjct: 68  LGSEEAAREALVYSYKNAASGFSARLTPDQVTEITKQPGVLHVVQSGNNKLHSGGGGVAR 127

[Close]

BLAST of MC11g1216 vs. ExPASy TrEMBL
Match: A0A6J1F6S6 (subtilisin-like protease SBT3.13 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442643 PE=4 SV=1)

HSP 1 Score: 176 bits (447), Expect = 9.23e-55
Identity = 93/122 (76.23%), Postives = 106/122 (86.89%), Query Frame = 0


Query: 3   LALLFSISLNFLLSSAAMSGQ---------YASPAVHIVYTEKPRDEEPEAYHIRTLVSV 62
           L LLFSISLN  LSSA MS Q         +ASPAVHIVYTE+P +EEPEAYHIRTL SV
Sbjct: 19  LVLLFSISLNLFLSSAVMSDQSASIPVVSNFASPAVHIVYTERPHNEEPEAYHIRTLASV 78

Query: 63  LGSEEAAKEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQLHSGGGGVSR 115
           LGSEEAA+EALVYSYKNAASGFSARLTP+QVA+++KQPGVLHVVQSG+++LH+GGGGV+R
Sbjct: 79  LGSEEAAREALVYSYKNAASGFSARLTPEQVADITKQPGVLHVVQSGSNKLHTGGGGVAR 138

[Close]

BLAST of MC11g1216 vs. ExPASy TrEMBL
Match: A0A1S3CEN1 (subtilisin-like protease SBT3.6 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500039 PE=4 SV=1)

HSP 1 Score: 174 bits (441), Expect = 5.33e-54
Identity = 94/122 (77.05%), Postives = 104/122 (85.25%), Query Frame = 0


Query: 3   LALLFSISLNFLLSSAAMSGQ---------YASPAVHIVYTEKPRDEEPEAYHIRTLVSV 62
           L  LFSISLN  LSSAAMS Q         + SPAVHIVYTE+P +E PEAYHIRTLVSV
Sbjct: 8   LLFLFSISLNLHLSSAAMSSQSSSIPLVSNFDSPAVHIVYTERPHNEAPEAYHIRTLVSV 67

Query: 63  LGSEEAAKEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQLHSGGGGVSR 115
           LGSEEAA+EALVYSYKNAASGFSARLTPDQV E++KQPGVLHVVQSG+++LHSGGGGV+R
Sbjct: 68  LGSEEAAREALVYSYKNAASGFSARLTPDQVKEITKQPGVLHVVQSGSNKLHSGGGGVAR 127

[Close]

BLAST of MC11g1216 vs. TAIR 10
Match: AT1G71950.1 (Proteinase inhibitor, propeptide )

HSP 1 Score: 129.4 bits (324), Expect = 1.8e-30
Identity = 68/117 (58.12%), Postives = 84/117 (71.79%), Query Frame = 0


Query: 1   LCLALLFSISLNFLLSSAAMSGQYASPA-------VHIVYTEKPRDEEPEAYHIRTLVSV 60
           + +   F   L+   +S  M+ +Y   A       VHI+YTEKP DEEP+ YH+RTL S 
Sbjct: 14  IIIVFFFFFCLSSFFASIVMADEYTGEATGSSEAKVHIIYTEKPTDEEPKTYHLRTLSSA 73

Query: 61  LGSEEAAKEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQLHSGGGG 111
           LGSEEAAK+AL+YSYK AASGFSA+LTP+QVAE+SKQPGV+ VV S T+QLH  GGG
Sbjct: 74  LGSEEAAKDALIYSYKEAASGFSAKLTPEQVAEISKQPGVIQVVPSQTYQLHKPGGG 130

[Close]

BLAST of MC11g1216 vs. TAIR 10
Match: AT5G11940.1 (Subtilase family protein )

HSP 1 Score: 65.9 bits (159), Expect = 2.5e-11
Identity = 34/88 (38.64%), Postives = 54/88 (61.36%), Query Frame = 0


Query: 22  GQYASPAVHIVYTEKPRDEEPE---AYHIRTLVSVLGSEEAAKEALVYSYKNAASGFSAR 81
           G Y    VHIVY  +    +PE   + H+R L S+LGS++ A E++V+SY+N  SGF+A 
Sbjct: 30  GAYEETKVHIVYLGEKEHNDPELVTSSHLRMLESLLGSKKDASESIVHSYRNGFSGFAAH 89

Query: 82  LTPDQVAEVSKQPGVLHVVQSGTHQLHS 107
           LT  Q  ++S+ P V+ V  +  ++L +
Sbjct: 90  LTDSQAEQISEHPDVVQVTPNTFYELQT 117

[Close]

BLAST of MC11g1216 vs. TAIR 10
Match: AT1G32960.1 (Subtilase family protein )

HSP 1 Score: 63.2 bits (152), Expect = 1.6e-10
Identity = 38/103 (36.89%), Postives = 63/103 (61.17%), Query Frame = 0


Query: 5   LLFSISLNFLLSSAAMSGQYASPAVHIVYTEKPRDEEPE---AYHIRTLVSVLGSEEAAK 64
           LL  +SL  +L++     +  S  VHIVY  + +  +PE     H + L S+LGS++ A 
Sbjct: 9   LLVLLSLITVLNATRARSETES-KVHIVYLGEKKHHDPEFVTESHHQMLASLLGSKKDAD 68

Query: 65  EALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQL 105
           +++VYSY++  SGF+A+LT  Q  +++  P V+HV+  G H+L
Sbjct: 69  DSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHEL 110

[Close]

BLAST of MC11g1216 vs. TAIR 10
Match: AT4G10550.3 (Subtilase family protein )

HSP 1 Score: 62.8 bits (151), Expect = 2.1e-10
Identity = 33/83 (39.76%), Postives = 53/83 (63.86%), Query Frame = 0


Query: 25  ASPAVHIVYTEKPRDEEPE---AYHIRTLVSVLGSEEAAKEALVYSYKNAASGFSARLTP 84
           A   VHIVY  + + ++PE     H R L S+LGS+E A +++VYSY++  SGF+A+LT 
Sbjct: 46  AKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLTE 105

Query: 85  DQVAEVSKQPGVLHVVQSGTHQL 105
            Q  +++  P V+HV+    ++L
Sbjct: 106 SQAKKIADLPDVVHVIPDSFYKL 128

[Close]

BLAST of MC11g1216 vs. TAIR 10
Match: AT4G10540.1 (Subtilase family protein )

HSP 1 Score: 61.2 bits (147), Expect = 6.1e-10
Identity = 42/107 (39.25%), Postives = 64/107 (59.81%), Query Frame = 0


Query: 2   CLALLF-SISLNFLLSSAAMSGQYASPAVHIVYTEKPRDEEPE---AYHIRTLVSVLGSE 61
           C  L+F +I LN L +  A +G  A   VHIVY  + + ++PE     H R L S+LGS+
Sbjct: 4   CRTLIFVAIILNGLSTFVAHAG--AESKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSK 63

Query: 62  EAAKEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQL 105
           E A  ++V+SY++  SGF+A+LT  Q  +++  P V+HV     +QL
Sbjct: 64  EDAHSSMVHSYRHGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQL 108

The following BLAST results are available for this feature:

BLAST of MC11g1216 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q1PDX5	3.5e-10	38.64	Subtilisin-like protease SBT3.11 OS=Arabidopsis thaliana OX=3702 GN=SBT3.11 PE=2...	[more]
Q9MAP5	2.3e-09	36.89	Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 S...	[more]
Q9SZY3	8.7e-09	39.25	Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 S...	[more]
Q8L7I2	1.1e-08	34.58	Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 S...	[more]
F4HPF1	1.5e-08	35.58	Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 S...	[more]

BLAST of MC11g1216 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143620.1	1.41e-71	100.00	subtilisin-like protease SBT3.11 isoform X1 [Momordica charantia]	[more]
XP_022143621.1	2.18e-60	100.00	subtilisin-like protease SBT3.11 isoform X2 [Momordica charantia] >XP_022143622....	[more]
XP_004139693.1	4.69e-55	78.69	subtilisin-like protease SBT3.6 isoform X1 [Cucumis sativus]	[more]
KAG7025065.1	1.63e-54	76.23	Subtilisin-like protease SBT3.6, partial [Cucurbita argyrosperma subsp. argyrosp...	[more]
XP_022935862.1	1.91e-54	76.23	subtilisin-like protease SBT3.13 isoform X1 [Cucurbita moschata]	[more]

BLAST of MC11g1216 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CPA2	6.84e-72	100.00	subtilisin-like protease SBT3.11 isoform X1 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1CQU0	1.05e-60	100.00	subtilisin-like protease SBT3.11 isoform X2 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A0A0K6B0	2.27e-55	78.69	Inhibitor I9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G29169...	[more]
A0A6J1F6S6	9.23e-55	76.23	subtilisin-like protease SBT3.13 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A1S3CEN1	5.33e-54	77.05	subtilisin-like protease SBT3.6 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1035000...	[more]

BLAST of MC11g1216 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G71950.1	1.8e-30	58.12	Proteinase inhibitor, propeptide	[more]
AT5G11940.1	2.5e-11	38.64	Subtilase family protein	[more]
AT1G32960.1	1.6e-10	36.89	Subtilase family protein	[more]
AT4G10550.3	2.1e-10	39.76	Subtilase family protein	[more]
AT4G10540.1	6.1e-10	39.25	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 15..105 e-value: 2.2E-22 score: 81.3
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 30..105 e-value: 9.8E-12 score: 45.4
None	No IPR available	PANTHER	PTHR48222	PROTEINASE INHIBITOR, PROPEPTIDE	coord: 11..107

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g1216.1	MC11g1216.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0008233	peptidase activity