MC11g1041 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g1041
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	meiotic recombination protein SPO11-1
Location	MC11: 8945555 .. 8949095 (+)
RNA-Seq Expression	MC11g1041
Synteny	MC11g1041

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGGAAAGCGAGCAAGCTCGGAGAGAAGCGACATTCTAAGAAGAATCAGAGGTTCCAATTCCATTCAATTTTCTCATTATCATATTGGTTCCAAACAATTACTGATTCGATTGTTGCTATTTTCCTTCCGCTTTTTAATTTCAACGTTTTTCAGAGATAACAAGATCTATGATCGTCGAGCTGAGCAACGGAAGCTCCAAGATGATGGCAATCGATCGGTTTAAAAATTACTGCACTAATTCTCTTGAAAACTGGTTCGGATCTTGTTTCGTTTGGTCGCAGTTTTGGTTTCTACTGAACCTGCAAATTAGCTACCAAATAAAGATCATAATCTATTACGATTCTATGTGATTTACTCGACTAGGAATGTTCATTTCCCAATCTGTATTCATTTTTGAATCATTTAGGATTCGTTATTTACTGCTGAGTTTCTTGGATCTTCCCGGAGGCCAGAACAGTTATCGTTTGTAAAATCCAGCCAAAAAAAAAAAAACCTAGTAGCAGTTCGGACGAGATTTTATCACTCGAAATTGAGGCACAAAGTTGCATAGTTCAAATCAGCATTTAACATTTACCACCTAGTTTTCAGTTTCGTGAAACACATTATGAGTTTTTAAGAATAATGTTTCTACTTGTCAATACAATGTATTTCTGGAACGTGAAGAGGTAGATCTTGAATTTGGACTTTAAATGCAGCTGTTGCAAGTTCAATTTATCCGAAGGGAAGGAGATTCTAACACTTCAAAAGGAAAGCCATGTCCGTAGACTAGGTGATTCAGTTATGTCAATGAATGATATTATAATCTGTATAAAGTACACCTGTGTCTTATTTGATCTTATTTTGCTCTATCAGATGTCTTGCTTAGAGTTCTACTCATTGTTCGGCAGCTTCTGCGAGAAAATAGACATAGCTCGAAGAGAGATATATATTATATGCATCCCTCTGTGTTTTCAGGTAAAGCTAACCCATCATTTTATCAGCAGAAACCAGTAGTTTTGTTCCAAGATACAATATCTTTCACAAAATAATAAGCACTCAAGTTCGGAAGGATCGGAATTCAACATATCCTTTAAATTAAAGTTTTCAGTAAGTTCGTGGCAGGAGATTTTCTCAACAATGCTTTGTATAGCATGCCAGATTTTATTGACATTGGCTTCTTCAAATAATATTTGCAGATCAGTCAGTTGTTGATCGGGCAATCAACGACATCTGCATTCTTTTGCAATGTAGTCGGCACAATCTAAATGTGGTTGGTGCCGCACTTCATTTTACTGTTTATTCAATTTGTGCTTTTTTTTTTCTTTTTTTCAATGATTATATAATGTGATTTATGGGGGATCTAATTTCTGTATGGTACTACTAACGGTTGGCTGCATATCTTAAATTGGCCGCATATTTTAGCTTTAATAAATAAACTTACAGTTGGTTTTTTGCTTTCAAACATCACTAACAGTTCAATTACAGTTTTCTTTAAGTTCCGTAGTTTGTGTCGCAAGCTTCTTCTGATAATAAAGTGTGTGATGATCATTTCAACTTGTGTCCTCTTGACAGGTTTCTTTTGGAAATGGGTACGTATTGAAGATATAATATGCTCAGTTCAGATAAGAAGAAAATAGTGACGAATGCTGTTTGTTTTCTTGACATGAGCTTTTACAAGCAGTCTGTATCACATAATTCTTTGTTATGTGGGTTGATACCTACCCATGTCAGGATTTTCTAAACCATTAGATTCTGTTAAAGAAAAACTTGTTGTTTTTGCTGCGCTCTGTTCCATTGAGGAAGAAAATTAAGAACTGAATCTTGACTTAGACACTTCTTTATAGGTTAGTGATGGGTTGGTTGAGGTTCTCAGAAGCTGGTAGGAGATTTGATTGCATAAAGAAGCCTAACAGTGTAAGCATTATACTTGAACTTGATTGTTCTTCATGATGCAATTGCTTCTTTTTTTAACTCTTAGCTGTACGTAATTTGGATATATGCATCTCAATCACTGCCAGGCCCACTCCATTCCTGTTCAAGTTGAGGAAGTCACAGGTATGCAGATACTGATTGATAAATCGCCAATCCACTGCGTTAGAACTTAAAATAGAAACTTTCTGAATGATATCTTTGGATATGGATTTCTATGCTGTATTCTGCCAAAGTTCGACATAAAATTACATGGTTTTTATCGTATTCCTCATAATCTTCAAAACTTTTGGCCTGAGATTCTGCTTTAGCTTGTTATTATTTTATTCTCATCTGCAGACATTGTTAGTGCTGCGGAATACATACTCATCGTTGAGAAGGAATCAGGTGAAGGTTATAGTGATAGAAGCTAGTTTTGCGGTTTAAATAAACTCAATTTTAAGTTTGACCTCGATCAGTCAATATATATGGTTTGATGTTTTCAATGTGCAGTTTTCCAGCGTTTAGCAAATGACCAGTTCTGCAGCAGAAACCGCTGCATTGTAATCACGGTGAGTATGTCTCTGGTCCTGCAACTTTGAAATTAGTTCAACAATAGGGTGGGGATTTGAACCTCCAAAATTTTGTTCATCAATGCATGCTTTAACTAGTCGAGTTACACTCGGTTAACTTGAAAATTCTCATATGTAAATGTTAGTTTTCATTTTCATTTTCAGGGACGTGGATATCCAGATGTTCCCACCAGAAGGTAAAGCAAAATATGTTCATACACACACATTTTTGGATGCTAATCTCATTTTACTGTTGGTGATGATTATATTATATGAATCATGGGAATATGCATGTATTAGTCAGGTTTTTGCGGCTTCTCGTTGATGTTTCGGCATTGCCTGCATTTTGCTTGGTAGATTGTGATCCATATGGTTTCGAAATTCTAACAACATATCGATTTGGTTCCATGGTTAGGCTCCTAAAACAATCATCTAAATCATTTATCTACTCAATTATGTTAAGAGAGCAGTGAACTAATCGACTATGTGGAACAATGAGACATTGAGAAGCATTTTTTTTTTTTCAGTTAAGGCTAAACTAACTTCCAGTTAGTGATTTTGACCCGTTTCCTTTTCACTAGCAAATGGCGTACGATGCAAAGTATCTCAGAATTCCACATCTACGCTGGCTTGGTGCTTTTGCTTCAGACTCTGAGAATTACAATCTTCCAGAACAATGTCTCATCCCATTGACTATGGAGGGTGAGGAATAATATATTCTAATACTCTCTATCTTTGCGGTTTCAAATGAAATTTTTCTGACCTTTTCTGAGGGTCTTGCTTATCCTGTCAGATAAGAGAAGAACCGAAGCCTTGTTACTCAGATGCTACTTGCAGAGAGAGGTACCACATTGGAGGTAAGCTTCTGTTAAGAACTCTAATTTTCTGTTGTCCTTGTCTTCATGCATATTGCCATTTTCTACAAAGAGGATTTGATCCCTTAGGTTGGAATTGGACTCAATGCTGCAAAAGGGTGTGAAATTTGAGATAGAAGCCCTCTCTGTGCACAATCTTTCCTTTTTGTCAGAAGAGTATTTACCATCCAAAATAGGATGTGTTCATCTTAGAACTGGTAAA

mRNA sequence

ATGGAGGGAAAGCGAGCAAGCTCGGAGAGAAGCGACATTCTAAGAAGAATCAGAGAGATAACAAGATCTATGATCGTCGAGCTGAGCAACGGAAGCTCCAAGATGATGGCAATCGATCGGTTTAAAAATTACTGCACTAATTCTCTTGAAAACTGCTGTTGCAAGTTCAATTTATCCGAAGGGAAGGAGATTCTAACACTTCAAAAGGAAAGCCATGTCCGTAGACTAGATGTCTTGCTTAGAGTTCTACTCATTGTTCGGCAGCTTCTGCGAGAAAATAGACATAGCTCGAAGAGAGATATATATTATATGCATCCCTCTGTGTTTTCAGATCAGTCAGTTGTTGATCGGGCAATCAACGACATCTGCATTCTTTTGCAATGTAGTCGGCACAATCTAAATGTGGTTTCTTTTGGAAATGGGTTAGTGATGGGTTGGTTGAGGTTCTCAGAAGCTGGTAGGAGATTTGATTGCATAAAGAAGCCTAACAGTGCCCACTCCATTCCTGTTCAAGTTGAGGAAGTCACAGACATTGTTAGTGCTGCGGAATACATACTCATCGTTGAGAAGGAATCAGTTTTCCAGCGTTTAGCAAATGACCAGTTCTGCAGCAGAAACCGCTGCATTGTAATCACGGGACGTGGATATCCAGATGTTCCCACCAGAAGGTTTTTGCGGCTTCTCGTTGATGTTTCGGCATTGCCTGCATTTTGCTTGGTAGATTGTGATCCATATGGTTTCGAAATTCTAACAACATATCGATTTGGTTCCATGCAAATGGCGTACGATGCAAAGTATCTCAGAATTCCACATCTACGCTGGCTTGGTGCTTTTGCTTCAGACTCTGAGAATTACAATCTTCCAGAACAATGTCTCATCCCATTGACTATGGAGGATAAGAGAAGAACCGAAGCCTTGTTACTCAGATGCTACTTGCAGAGAGAGGTACCACATTGGAGGTTGGAATTGGACTCAATGCTGCAAAAGGGTGTGAAATTTGAGATAGAAGCCCTCTCTGTGCACAATCTTTCCTTTTTGTCAGAAGAGTATTTACCATCCAAAATAGGATGTGTTCATCTTAGAACTGGTAAA

Coding sequence (CDS)

ATGGAGGGAAAGCGAGCAAGCTCGGAGAGAAGCGACATTCTAAGAAGAATCAGAGAGATAACAAGATCTATGATCGTCGAGCTGAGCAACGGAAGCTCCAAGATGATGGCAATCGATCGGTTTAAAAATTACTGCACTAATTCTCTTGAAAACTGCTGTTGCAAGTTCAATTTATCCGAAGGGAAGGAGATTCTAACACTTCAAAAGGAAAGCCATGTCCGTAGACTAGATGTCTTGCTTAGAGTTCTACTCATTGTTCGGCAGCTTCTGCGAGAAAATAGACATAGCTCGAAGAGAGATATATATTATATGCATCCCTCTGTGTTTTCAGATCAGTCAGTTGTTGATCGGGCAATCAACGACATCTGCATTCTTTTGCAATGTAGTCGGCACAATCTAAATGTGGTTTCTTTTGGAAATGGGTTAGTGATGGGTTGGTTGAGGTTCTCAGAAGCTGGTAGGAGATTTGATTGCATAAAGAAGCCTAACAGTGCCCACTCCATTCCTGTTCAAGTTGAGGAAGTCACAGACATTGTTAGTGCTGCGGAATACATACTCATCGTTGAGAAGGAATCAGTTTTCCAGCGTTTAGCAAATGACCAGTTCTGCAGCAGAAACCGCTGCATTGTAATCACGGGACGTGGATATCCAGATGTTCCCACCAGAAGGTTTTTGCGGCTTCTCGTTGATGTTTCGGCATTGCCTGCATTTTGCTTGGTAGATTGTGATCCATATGGTTTCGAAATTCTAACAACATATCGATTTGGTTCCATGCAAATGGCGTACGATGCAAAGTATCTCAGAATTCCACATCTACGCTGGCTTGGTGCTTTTGCTTCAGACTCTGAGAATTACAATCTTCCAGAACAATGTCTCATCCCATTGACTATGGAGGATAAGAGAAGAACCGAAGCCTTGTTACTCAGATGCTACTTGCAGAGAGAGGTACCACATTGGAGGTTGGAATTGGACTCAATGCTGCAAAAGGGTGTGAAATTTGAGATAGAAGCCCTCTCTGTGCACAATCTTTCCTTTTTGTCAGAAGAGTATTTACCATCCAAAATAGGATGTGTTCATCTTAGAACTGGTAAA

Protein sequence

MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSEGKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAINDICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVSAAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLVDCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDKRRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHLRTGK

Homology

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BLAST of MC11g1041 vs. ExPASy Swiss-Prot
Match: Q9M4A2 (Meiotic recombination protein SPO11-1 OS=Arabidopsis thaliana OX=3702 GN=SPO11-1 PE=1 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 2.6e-144
Identity = 245/355 (69.01%), Postives = 300/355 (84.51%), Query Frame = 0


Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           MEGK A SE +++L+RI++ T+S++V+L+ G S  ++I++F+NYC N   +C C  +  +
Sbjct: 1   MEGKFAISESTNLLQRIKDFTQSVVVDLAEGRSPKISINQFRNYCMNPEADCLCSSDKPK 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           G+EI TL+KE    R+D+LLRVLLIV+QLL+ENRH+SKRDIYYMHPS F  QS+VDRAI 
Sbjct: 61  GQEIFTLKKEPQTYRIDMLLRVLLIVQQLLQENRHASKRDIYYMHPSAFKAQSIVDRAIG 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICIL QCSR+NLNVVS GNGLVMGWL+F EAGR+FDC+   N+A+ +PV VEEV DIVS
Sbjct: 121 DICILFQCSRYNLNVVSVGNGLVMGWLKFREAGRKFDCLNSLNTAYPVPVLVEEVEDIVS 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
            AEYIL+VEKE+VFQRLAND FC  NRCIVITGRGYPDV TRRFLRLL++   LP  CLV
Sbjct: 181 LAEYILVVEKETVFQRLANDMFCKTNRCIVITGRGYPDVSTRRFLRLLMEKLHLPVHCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGFEIL TYRFGSMQMAYD + LR P ++WLGAF SDSE Y++P+QCL+PLT EDK
Sbjct: 241 DCDPYGFEILATYRFGSMQMAYDIESLRAPDMKWLGAFPSDSEVYSVPKQCLLPLTEEDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 356
           +RTEA+LLRCYL+RE+P WRLEL++ML++GVKFEIEALSVH+LSFLSE Y+PSKI
Sbjct: 301 KRTEAMLLRCYLKREMPQWRLELETMLKRGVKFEIEALSVHSLSFLSEVYIPSKI 355

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BLAST of MC11g1041 vs. ExPASy Swiss-Prot
Match: Q5ZPV8 (Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. indica OX=39946 GN=SPO11-1 PE=2 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.1e-126
Identity = 213/359 (59.33%), Postives = 279/359 (77.72%), Query Frame = 0


Query: 2   EGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTN----SLENCCCKFN 61
           E +R   E + +L RIR + R ++ E++ G S  +A+ R++NYC++    +   C C ++
Sbjct: 17  ERRRRQEEAATLLHRIRGLVRWVVAEVAAGRSPTVALHRYQNYCSSASAAAASPCACSYD 76

Query: 62  LSEGKEILT-LQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVD 121
           +  G ++L+ L + SH  RL+VLLRVLL+V+QLL++N+H SKRDIYYM+PS+F +Q+VVD
Sbjct: 77  VPVGTDVLSLLHRGSHASRLNVLLRVLLVVQQLLQQNKHCSKRDIYYMYPSIFQEQAVVD 136

Query: 122 RAINDICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVT 181
           RAINDIC+L +CSRHNLNVV    GLVMGW+RF E  +   C+   N+A SIPV +E + 
Sbjct: 137 RAINDICVLFKCSRHNLNVVPVAKGLVMGWIRFLEGEKEVYCVTNVNAAFSIPVSIEAIK 196

Query: 182 DIVSAAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPA 241
           D+VS A+YILIVEKE+VFQRLAND+FC RNRCIVITGRGYPD+PTRRFLR LV+   LP 
Sbjct: 197 DVVSVADYILIVEKETVFQRLANDKFCERNRCIVITGRGYPDIPTRRFLRYLVEQLHLPV 256

Query: 242 FCLVDCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLT 301
           +CLVD DPYGF+IL TY+FGS+Q+AYDA +LR+P +RWLG F SD E+Y LP+ CL+ L+
Sbjct: 257 YCLVDADPYGFDILATYKFGSLQLAYDANFLRVPDIRWLGVFTSDFEDYRLPDCCLLHLS 316

Query: 302 MEDKRRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 356
            ED+R+ E +L RCYL RE P WRLEL++MLQKGVKFEIEALS  ++SFLSEEY+P KI
Sbjct: 317 SEDRRKAEGILSRCYLHREAPQWRLELEAMLQKGVKFEIEALSACSISFLSEEYIPKKI 375

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BLAST of MC11g1041 vs. ExPASy Swiss-Prot
Match: Q7Y021 (Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. japonica OX=39947 GN=SPO11-1 PE=2 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.1e-126
Identity = 213/359 (59.33%), Postives = 279/359 (77.72%), Query Frame = 0


Query: 2   EGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTN----SLENCCCKFN 61
           E +R   E + +L RIR + R ++ E++ G S  +A+ R++NYC++    +   C C ++
Sbjct: 17  ERRRRQEEAATLLHRIRGLVRWVVAEVAAGRSPTVALHRYQNYCSSASAAAASPCACSYD 76

Query: 62  LSEGKEILT-LQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVD 121
           +  G ++L+ L + SH  RL+VLLRVLL+V+QLL++N+H SKRDIYYM+PS+F +Q+VVD
Sbjct: 77  VPVGTDVLSLLHRGSHASRLNVLLRVLLVVQQLLQQNKHCSKRDIYYMYPSIFQEQAVVD 136

Query: 122 RAINDICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVT 181
           RAINDIC+L +CSRHNLNVV    GLVMGW+RF E  +   C+   N+A SIPV +E + 
Sbjct: 137 RAINDICVLFKCSRHNLNVVPVAKGLVMGWIRFLEGEKEVYCVTNVNAAFSIPVSIEAIK 196

Query: 182 DIVSAAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPA 241
           D+VS A+YILIVEKE+VFQRLAND+FC RNRCIVITGRGYPD+PTRRFLR LV+   LP 
Sbjct: 197 DVVSVADYILIVEKETVFQRLANDKFCERNRCIVITGRGYPDIPTRRFLRYLVEQLHLPV 256

Query: 242 FCLVDCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLT 301
           +CLVD DPYGF+IL TY+FGS+Q+AYDA +LR+P +RWLG F SD E+Y LP+ CL+ L+
Sbjct: 257 YCLVDADPYGFDILATYKFGSLQLAYDANFLRVPDIRWLGVFTSDFEDYRLPDCCLLHLS 316

Query: 302 MEDKRRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 356
            ED+R+ E +L RCYL RE P WRLEL++MLQKGVKFEIEALS  ++SFLSEEY+P KI
Sbjct: 317 SEDRRKAEGILSRCYLHREAPQWRLELEAMLQKGVKFEIEALSACSISFLSEEYIPKKI 375

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BLAST of MC11g1041 vs. ExPASy Swiss-Prot
Match: Q9Y5K1 (Meiotic recombination protein SPO11 OS=Homo sapiens OX=9606 GN=SPO11 PE=1 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.8e-60
Identity = 130/363 (35.81%), Postives = 225/363 (61.98%), Query Frame = 0


Query: 3   GKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSEGK 62
           G R +S  S++L  I  I + +I  L+   +    ID       +S EN   KF  S G 
Sbjct: 38  GSRLASS-SEVLASIENIIQDIITSLARNEAPAFTIDN-----RSSWEN--IKFEDSVGL 97

Query: 63  EILT------LQKES--HVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSV 122
           ++++      ++ +S    ++  ++L++L ++ +L++ N +++KRDIYY    +F +Q+V
Sbjct: 98  QMVSHCTTRKIKSDSPKSAQKFSLILKILSMIYKLVQSNTYATKRDIYYTDSQLFGNQTV 157

Query: 123 VDRAINDICILLQCSRHNLNVVSFGNGLVMGWLRF-SEAGRRFDCIKKPNSAHSIPVQVE 182
           VD  INDI  +L+ SR +L+++S   GL+ G LR+  E G + +C     +A ++P  ++
Sbjct: 158 VDNIINDISCMLKVSRRSLHILSTSKGLIAGNLRYIEEDGTKVNCTCGA-TAVAVPSNIQ 217

Query: 183 EVTDIVSAAEYILIVEKESVFQRLANDQFCSR-NRCIVITGRGYPDVPTRRFLRLLVDVS 242
            + ++V+ A+++LIVEK++ FQRL +D FC++ + CI+ITG+G PD+ TR  ++ L D  
Sbjct: 218 GIRNLVTDAKFVLIVEKDATFQRLLDDNFCNKLSPCIMITGKGVPDLNTRLLVKKLWDTF 277

Query: 243 ALPAFCLVDCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCL 302
            +P F LVD DP+G EI+  Y++GSM M+++A +L +P +RWLG   SD +  N+P+  L
Sbjct: 278 HVPVFTLVDADPHGIEIMCIYKYGSMSMSFEAHHLTVPAIRWLGLLPSDLKRLNVPKDSL 337

Query: 303 IPLTMEDKRRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLP 356
           IPLT  D+ + +++L R Y+  + P WR E++ M    +K EI+AL+  +  +LS  YLP
Sbjct: 338 IPLTKRDQMKLDSILRRPYVTCQ-PFWRKEMEIMADSKMKAEIQALTFLSSDYLSRVYLP 390

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BLAST of MC11g1041 vs. ExPASy Swiss-Prot
Match: Q9WTK8 (Meiotic recombination protein SPO11 OS=Mus musculus OX=10090 GN=Spo11 PE=1 SV=2)

HSP 1 Score: 230.3 bits (586), Expect = 3.4e-59
Identity = 131/363 (36.09%), Postives = 217/363 (59.78%), Query Frame = 0


Query: 3   GKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSEGK 62
           G    +  S++L  I  I + +I  L+        ID       +S EN    F+ S G 
Sbjct: 37  GATRVASSSEVLTAIENIIQDIIKSLARNEVPAFTIDN-----RSSWEN--IMFDDSVGL 96

Query: 63  EIL---TLQK-----ESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSV 122
            ++   T +K        V++  ++L+VL ++ +L++ + +++KRDIYY    +F +Q+ 
Sbjct: 97  RMIPQCTTRKIRSDSPKSVKKFALILKVLSMIYKLIQSDTYATKRDIYYTDSQLFGNQAA 156

Query: 123 VDRAINDICILLQCSRHNLNVVSFGNGLVMGWLRF-SEAGRRFDCIKKPNSAHSIPVQVE 182
           VD AI+DI  +L+  R +L+V+S   GL+ G LR+  E G R  C     +A ++P  ++
Sbjct: 157 VDSAIDDISCMLKVPRRSLHVLSTSKGLIAGNLRYMEEDGTRVQCTCSA-TATAVPTNIQ 216

Query: 183 EVTDIVSAAEYILIVEKESVFQRLANDQFCSR-NRCIVITGRGYPDVPTRRFLRLLVDVS 242
            +  +++ A+++LIVEK++ FQRL +D FCSR + CI++TG+G PD+ TR  ++ L D  
Sbjct: 217 GMQHLITDAKFLLIVEKDATFQRLLDDNFCSRMSPCIMVTGKGVPDLNTRLLVKKLWDTF 276

Query: 243 ALPAFCLVDCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCL 302
            +P F LVD DPYG EI+  Y++GSM M+++A  L IP +RWLG   SD +  N+P+  L
Sbjct: 277 HIPVFTLVDADPYGIEIMCIYKYGSMSMSFEAHNLTIPTIRWLGLLPSDIQRLNIPKDSL 336

Query: 303 IPLTMEDKRRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLP 356
           IPLT  D+ + +++L R Y+  + P W+ EL+ M    +K EI+AL++ +  +LS  YLP
Sbjct: 337 IPLTKHDQMKLDSILKRPYITYQ-PLWKKELEMMADSKMKAEIQALTLLSSDYLSRVYLP 390

[Close]

BLAST of MC11g1041 vs. NCBI nr
Match: XP_022139661.1 (meiotic recombination protein SPO11-1 [Momordica charantia])

HSP 1 Score: 731 bits (1887), Expect = 4.87e-266
Identity = 364/364 (100.00%), Postives = 364/364 (100.00%), Query Frame = 0


Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE
Sbjct: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN
Sbjct: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS
Sbjct: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
           AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV
Sbjct: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK
Sbjct: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHL 360
           RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHL
Sbjct: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHL 360

Query: 361 RTGK 364
           RTGK
Sbjct: 361 RTGK 364

[Close]

BLAST of MC11g1041 vs. NCBI nr
Match: XP_023536168.1 (meiotic recombination protein SPO11-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 651 bits (1679), Expect = 2.63e-234
Identity = 318/355 (89.58%), Postives = 339/355 (95.49%), Query Frame = 0


Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           M GK  SSER DI+R+IRE+TRSMIVEL N SSKM+AIDRFKNYCTNS+ENCCC+FNLSE
Sbjct: 1   MAGKLGSSERIDIMRKIRELTRSMIVELCNRSSKMIAIDRFKNYCTNSIENCCCRFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           GKEILTLQ+ESHVRRLDVLLRVLLI++QLLRENRHSSKRDIYYMHPSVF+DQSVVDRAIN
Sbjct: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICILLQCSRHNLNVVS GNGLVMGWLRF+EAGRR DCIKKPNSAHSIPVQVEEVTDI S
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRSDCIKKPNSAHSIPVQVEEVTDIAS 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
            A YIL+VEKESVFQRLAND FCSRNRCIVI+GRGYPDVPTRRFLRLLVDV ALPAFCLV
Sbjct: 181 PANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGF+ILTTYRFGSMQMAYDAKYLRIP L WLGAFASDS+ YNLP+QCLIPLT+EDK
Sbjct: 241 DCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLHWLGAFASDSDTYNLPQQCLIPLTLEDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 355
           RRTEALLLRCYL+REVPHWRLEL+SMLQ+GVKFEIEALSVHN+SFLSEEYLPSKI
Sbjct: 301 RRTEALLLRCYLRREVPHWRLELESMLQRGVKFEIEALSVHNISFLSEEYLPSKI 355

[Close]

BLAST of MC11g1041 vs. NCBI nr
Match: XP_022976331.1 (meiotic recombination protein SPO11-1 [Cucurbita maxima])

HSP 1 Score: 650 bits (1676), Expect = 6.50e-234
Identity = 317/355 (89.30%), Postives = 338/355 (95.21%), Query Frame = 0


Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           M G    SER DI+R+IRE+TRSMIVELSNGSSKM+AIDRFKNYC NS+ENCCC+FNLSE
Sbjct: 1   MAGNLGRSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCINSIENCCCRFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           GKEILTLQ+ESHVRRLDVLLRVLLI++QLLRENRHSSKRDIYYMHPSVF+DQSVVDRAIN
Sbjct: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICILLQCSRHNLNVVS GNGLVMGWLRF+EAGRR DCIKKPNSAHSIPVQVEEVTDI S
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRSDCIKKPNSAHSIPVQVEEVTDIAS 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
            A YIL+VEKESVFQRLAND FCSRNRCIVI+GRGYPDVPTRRFLRLLVDV ALPAFCLV
Sbjct: 181 PANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGF+ILTTYRFGSMQMAYDAKYLRIP L WLGAFASDS+ YNLP+QCLIPLT+EDK
Sbjct: 241 DCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLHWLGAFASDSDTYNLPQQCLIPLTLEDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 355
           RRTEALLLRCYL+REVPHWRLEL+SMLQ+GVKFEIEALSVHN+SFLSEEYLPSKI
Sbjct: 301 RRTEALLLRCYLRREVPHWRLELESMLQRGVKFEIEALSVHNISFLSEEYLPSKI 355

[Close]

BLAST of MC11g1041 vs. NCBI nr
Match: XP_022937001.1 (meiotic recombination protein SPO11-1 [Cucurbita moschata])

HSP 1 Score: 644 bits (1661), Expect = 1.57e-231
Identity = 316/357 (88.52%), Postives = 337/357 (94.40%), Query Frame = 0


Query: 1   MEGKRASSERS--DILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNL 60
           M GK  SSER   DI+R+IRE+TRSMIVELSNGSSKM+AIDRFKNYCTNS ENCCC+FNL
Sbjct: 1   MAGKLGSSERIRIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENCCCRFNL 60

Query: 61  SEGKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRA 120
           SEGKEILTLQ++SHVRRLDVLLRVLLI++QLLRENRHSSKRDIYYMHPSVF+DQS VDRA
Sbjct: 61  SEGKEILTLQRQSHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSAVDRA 120

Query: 121 INDICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDI 180
           INDICILLQCSRHNLNVVS GNGLVMGWLRF+EAGRRFDCIKKPN+AHSIPVQVEEVTDI
Sbjct: 121 INDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDI 180

Query: 181 VSAAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFC 240
              A YIL+VEKESVFQRLAND FCSRNRCIVI+GRGYPDVPTRRFLRLLVDV ALPAFC
Sbjct: 181 AGPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFC 240

Query: 241 LVDCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTME 300
           LVDCDPYGF+ILTTYRFGSMQMAYDAKYLRIP L WLGAFASDS  YNLP+QCLIPLT+E
Sbjct: 241 LVDCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLHWLGAFASDSNTYNLPQQCLIPLTLE 300

Query: 301 DKRRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 355
           DKRRTEALL RCYL+REVPHWRLEL+SMLQ+GVKFEIEALSVHN+SFLSEEYLPSKI
Sbjct: 301 DKRRTEALLPRCYLRREVPHWRLELESMLQRGVKFEIEALSVHNISFLSEEYLPSKI 357

[Close]

BLAST of MC11g1041 vs. NCBI nr
Match: XP_008462989.1 (PREDICTED: meiotic recombination protein SPO11-1 isoform X1 [Cucumis melo])

HSP 1 Score: 642 bits (1657), Expect = 1.20e-230
Identity = 314/364 (86.26%), Postives = 343/364 (94.23%), Query Frame = 0


Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           MEG R S ER ++LR+I E+TRSMIVELSNGSSKM+AIDRFK+YCTNSLENCCC+FNLSE
Sbjct: 1   MEGNRGSLERIEVLRKITELTRSMIVELSNGSSKMIAIDRFKSYCTNSLENCCCRFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           GK++LTLQ+ESHVRRLDVLLRVLLI++QLLRENRHSSKRD+YYMHPS+F+DQSVVDRAIN
Sbjct: 61  GKDVLTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDMYYMHPSIFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICILLQCSRHNLNVVS GNGLVMGWLRFSEAGRRFDC+KKPNSA  IPVQVEEVTDI +
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAQFIPVQVEEVTDIAT 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
           AA+YIL+VEKESVFQRLAND+FCSRNRCIVITGRGYPDVPTRRFLRLLVDV ALPAFCLV
Sbjct: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGF+ILTTYRFGSMQMAYDAK LRIP L+W+GAFASDSE Y+LPEQCLIPLT +DK
Sbjct: 241 DCDPYGFDILTTYRFGSMQMAYDAKNLRIPQLQWIGAFASDSEKYDLPEQCLIPLTSKDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHL 360
           RRTEALL RCYL+REVPHWRLELDSMLQ+GVKFEIEALSVHN+SFLS+ YLPSKI    L
Sbjct: 301 RRTEALLQRCYLRREVPHWRLELDSMLQRGVKFEIEALSVHNISFLSDHYLPSKIES-EL 360

Query: 361 RTGK 364
            TGK
Sbjct: 361 TTGK 363

[Close]

BLAST of MC11g1041 vs. ExPASy TrEMBL
Match: A0A6J1CDC7 (meiotic recombination protein SPO11-1 OS=Momordica charantia OX=3673 GN=LOC111010510 PE=3 SV=1)

HSP 1 Score: 731 bits (1887), Expect = 2.36e-266
Identity = 364/364 (100.00%), Postives = 364/364 (100.00%), Query Frame = 0


Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE
Sbjct: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN
Sbjct: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS
Sbjct: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
           AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV
Sbjct: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK
Sbjct: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHL 360
           RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHL
Sbjct: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHL 360

Query: 361 RTGK 364
           RTGK
Sbjct: 361 RTGK 364

[Close]

BLAST of MC11g1041 vs. ExPASy TrEMBL
Match: A0A6J1IGM0 (meiotic recombination protein SPO11-1 OS=Cucurbita maxima OX=3661 GN=LOC111476764 PE=3 SV=1)

HSP 1 Score: 650 bits (1676), Expect = 3.15e-234
Identity = 317/355 (89.30%), Postives = 338/355 (95.21%), Query Frame = 0


Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           M G    SER DI+R+IRE+TRSMIVELSNGSSKM+AIDRFKNYC NS+ENCCC+FNLSE
Sbjct: 1   MAGNLGRSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCINSIENCCCRFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           GKEILTLQ+ESHVRRLDVLLRVLLI++QLLRENRHSSKRDIYYMHPSVF+DQSVVDRAIN
Sbjct: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICILLQCSRHNLNVVS GNGLVMGWLRF+EAGRR DCIKKPNSAHSIPVQVEEVTDI S
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRSDCIKKPNSAHSIPVQVEEVTDIAS 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
            A YIL+VEKESVFQRLAND FCSRNRCIVI+GRGYPDVPTRRFLRLLVDV ALPAFCLV
Sbjct: 181 PANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGF+ILTTYRFGSMQMAYDAKYLRIP L WLGAFASDS+ YNLP+QCLIPLT+EDK
Sbjct: 241 DCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLHWLGAFASDSDTYNLPQQCLIPLTLEDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 355
           RRTEALLLRCYL+REVPHWRLEL+SMLQ+GVKFEIEALSVHN+SFLSEEYLPSKI
Sbjct: 301 RRTEALLLRCYLRREVPHWRLELESMLQRGVKFEIEALSVHNISFLSEEYLPSKI 355

[Close]

BLAST of MC11g1041 vs. ExPASy TrEMBL
Match: A0A6J1F921 (meiotic recombination protein SPO11-1 OS=Cucurbita moschata OX=3662 GN=LOC111443423 PE=3 SV=1)

HSP 1 Score: 644 bits (1661), Expect = 7.59e-232
Identity = 316/357 (88.52%), Postives = 337/357 (94.40%), Query Frame = 0


Query: 1   MEGKRASSERS--DILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNL 60
           M GK  SSER   DI+R+IRE+TRSMIVELSNGSSKM+AIDRFKNYCTNS ENCCC+FNL
Sbjct: 1   MAGKLGSSERIRIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENCCCRFNL 60

Query: 61  SEGKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRA 120
           SEGKEILTLQ++SHVRRLDVLLRVLLI++QLLRENRHSSKRDIYYMHPSVF+DQS VDRA
Sbjct: 61  SEGKEILTLQRQSHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSAVDRA 120

Query: 121 INDICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDI 180
           INDICILLQCSRHNLNVVS GNGLVMGWLRF+EAGRRFDCIKKPN+AHSIPVQVEEVTDI
Sbjct: 121 INDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNAHSIPVQVEEVTDI 180

Query: 181 VSAAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFC 240
              A YIL+VEKESVFQRLAND FCSRNRCIVI+GRGYPDVPTRRFLRLLVDV ALPAFC
Sbjct: 181 AGPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFC 240

Query: 241 LVDCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTME 300
           LVDCDPYGF+ILTTYRFGSMQMAYDAKYLRIP L WLGAFASDS  YNLP+QCLIPLT+E
Sbjct: 241 LVDCDPYGFDILTTYRFGSMQMAYDAKYLRIPQLHWLGAFASDSNTYNLPQQCLIPLTLE 300

Query: 301 DKRRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 355
           DKRRTEALL RCYL+REVPHWRLEL+SMLQ+GVKFEIEALSVHN+SFLSEEYLPSKI
Sbjct: 301 DKRRTEALLPRCYLRREVPHWRLELESMLQRGVKFEIEALSVHNISFLSEEYLPSKI 357

[Close]

BLAST of MC11g1041 vs. ExPASy TrEMBL
Match: A0A1S3CJR6 (meiotic recombination protein SPO11-1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501238 PE=3 SV=1)

HSP 1 Score: 642 bits (1657), Expect = 5.80e-231
Identity = 314/364 (86.26%), Postives = 343/364 (94.23%), Query Frame = 0


Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           MEG R S ER ++LR+I E+TRSMIVELSNGSSKM+AIDRFK+YCTNSLENCCC+FNLSE
Sbjct: 1   MEGNRGSLERIEVLRKITELTRSMIVELSNGSSKMIAIDRFKSYCTNSLENCCCRFNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           GK++LTLQ+ESHVRRLDVLLRVLLI++QLLRENRHSSKRD+YYMHPS+F+DQSVVDRAIN
Sbjct: 61  GKDVLTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDMYYMHPSIFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICILLQCSRHNLNVVS GNGLVMGWLRFSEAGRRFDC+KKPNSA  IPVQVEEVTDI +
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAQFIPVQVEEVTDIAT 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
           AA+YIL+VEKESVFQRLAND+FCSRNRCIVITGRGYPDVPTRRFLRLLVDV ALPAFCLV
Sbjct: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGF+ILTTYRFGSMQMAYDAK LRIP L+W+GAFASDSE Y+LPEQCLIPLT +DK
Sbjct: 241 DCDPYGFDILTTYRFGSMQMAYDAKNLRIPQLQWIGAFASDSEKYDLPEQCLIPLTSKDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHL 360
           RRTEALL RCYL+REVPHWRLELDSMLQ+GVKFEIEALSVHN+SFLS+ YLPSKI    L
Sbjct: 301 RRTEALLQRCYLRREVPHWRLELDSMLQRGVKFEIEALSVHNISFLSDHYLPSKIES-EL 360

Query: 361 RTGK 364
            TGK
Sbjct: 361 TTGK 363

[Close]

BLAST of MC11g1041 vs. ExPASy TrEMBL
Match: A0A1S3CI64 (meiotic recombination protein SPO11-1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103501238 PE=3 SV=1)

HSP 1 Score: 625 bits (1613), Expect = 2.61e-224
Identity = 311/364 (85.44%), Postives = 339/364 (93.13%), Query Frame = 0


Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           MEG R S ER ++LR+I E+TRSMIVELSNGSSKM+AIDRFK+YCTNSLEN    FNLSE
Sbjct: 1   MEGNRGSLERIEVLRKITELTRSMIVELSNGSSKMIAIDRFKSYCTNSLENW---FNLSE 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           GK++LTLQ+ESHVRRLDVLLRVLLI++QLLRENRHSSKRD+YYMHPS+F+DQSVVDRAIN
Sbjct: 61  GKDVLTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDMYYMHPSIFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICILLQCSRHNLNVVS GNGLVMGWLRFSEAGRRFDC+KKPNSA  IPVQVEEVTDI +
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPNSAQFIPVQVEEVTDIAT 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
           AA+YIL+VEKESVFQRLAND+FCSRNRCIVITGRGYPDVPTRRFLRLLVDV ALPAFCLV
Sbjct: 181 AADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALPAFCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGF+ILTTYRFGSMQMAYDAK LRIP L+W+GAFASDSE Y+LPEQCLIPLT +DK
Sbjct: 241 DCDPYGFDILTTYRFGSMQMAYDAKNLRIPQLQWIGAFASDSEKYDLPEQCLIPLTSKDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKIGCVHL 360
           RRTEALL RCYL+REVPHWRLELDSMLQ+GVKFEIEALSVHN+SFLS+ YLPSKI    L
Sbjct: 301 RRTEALLQRCYLRREVPHWRLELDSMLQRGVKFEIEALSVHNISFLSDHYLPSKIES-EL 360

Query: 361 RTGK 364
            TGK
Sbjct: 361 TTGK 360

[Close]

BLAST of MC11g1041 vs. TAIR 10
Match: AT3G13170.1 (Spo11/DNA topoisomerase VI, subunit A protein )

HSP 1 Score: 513.1 bits (1320), Expect = 1.9e-145
Identity = 245/355 (69.01%), Postives = 300/355 (84.51%), Query Frame = 0


Query: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60
           MEGK A SE +++L+RI++ T+S++V+L+ G S  ++I++F+NYC N   +C C  +  +
Sbjct: 1   MEGKFAISESTNLLQRIKDFTQSVVVDLAEGRSPKISINQFRNYCMNPEADCLCSSDKPK 60

Query: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120
           G+EI TL+KE    R+D+LLRVLLIV+QLL+ENRH+SKRDIYYMHPS F  QS+VDRAI 
Sbjct: 61  GQEIFTLKKEPQTYRIDMLLRVLLIVQQLLQENRHASKRDIYYMHPSAFKAQSIVDRAIG 120

Query: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVS 180
           DICIL QCSR+NLNVVS GNGLVMGWL+F EAGR+FDC+   N+A+ +PV VEEV DIVS
Sbjct: 121 DICILFQCSRYNLNVVSVGNGLVMGWLKFREAGRKFDCLNSLNTAYPVPVLVEEVEDIVS 180

Query: 181 AAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLV 240
            AEYIL+VEKE+VFQRLAND FC  NRCIVITGRGYPDV TRRFLRLL++   LP  CLV
Sbjct: 181 LAEYILVVEKETVFQRLANDMFCKTNRCIVITGRGYPDVSTRRFLRLLMEKLHLPVHCLV 240

Query: 241 DCDPYGFEILTTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDK 300
           DCDPYGFEIL TYRFGSMQMAYD + LR P ++WLGAF SDSE Y++P+QCL+PLT EDK
Sbjct: 241 DCDPYGFEILATYRFGSMQMAYDIESLRAPDMKWLGAFPSDSEVYSVPKQCLLPLTEEDK 300

Query: 301 RRTEALLLRCYLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 356
           +RTEA+LLRCYL+RE+P WRLEL++ML++GVKFEIEALSVH+LSFLSE Y+PSKI
Sbjct: 301 KRTEAMLLRCYLKREMPQWRLELETMLKRGVKFEIEALSVHSLSFLSEVYIPSKI 355

[Close]

BLAST of MC11g1041 vs. TAIR 10
Match: AT5G02820.1 (Spo11/DNA topoisomerase VI, subunit A protein )

HSP 1 Score: 226.5 bits (576), Expect = 3.5e-59
Identity = 114/285 (40.00%), Postives = 174/285 (61.05%), Query Frame = 0


Query: 71  SHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAINDICILLQCSR 130
           S VR+  +  R+L ++ QL   N H +KRD++Y    +F DQ+  D  ++D+  +L C+R
Sbjct: 138 SSVRKTTITTRILALIHQLCLRNIHVTKRDLFYTDVKLFQDQTQSDAVLDDVSCMLGCTR 197

Query: 131 HNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPNSAHSIPVQVEEVTDIVSAAEYILIVEK 190
            +LNV++   G+V+G L FS+ G   DC K      +IP  ++ V D+ S A +IL+VEK
Sbjct: 198 SSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQSDAMFILLVEK 257

Query: 191 ESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALPAFCLVDCDPYGFEIL 250
           ++ + RLA D+F +R  CI++T +G PDV TR FLR +     LP   LVD DPYG +IL
Sbjct: 258 DAAYMRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELKLPVLALVDSDPYGLKIL 317

Query: 251 TTYRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDKRRTEALLLRC 310
           + Y  GS  M+YD+  L  P ++WLG   SD + Y +PEQC +P+T +D +  + +L   
Sbjct: 318 SVYGCGSKNMSYDSANLTTPDIKWLGIRPSDLDKYKIPEQCRLPMTEQDIKTGKDMLEED 377

Query: 311 YLQREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 356
           ++++  P W  EL+ M++   K EI+ALS     +LSE YLP K+
Sbjct: 378 FVKKN-PGWVEELNLMVKTKQKAEIQALSSFGFQYLSEVYLPLKL 421

[Close]

BLAST of MC11g1041 vs. TAIR 10
Match: AT1G63990.1 (sporulation 11-2 )

HSP 1 Score: 152.9 bits (385), Expect = 4.9e-37
Identity = 98/283 (34.63%), Postives = 160/283 (56.54%), Query Frame = 0


Query: 81  RVLLIVRQLLRENRHSSKRDIYYM----HPSVFSDQSVVDRAINDICILLQCSRHNLNVV 140
           +V+ I  Q+L + +  ++R+++Y      P  FS Q  V+R++ D+  LL+CSR++L ++
Sbjct: 102 KVMEICFQILLQEKRVTQRELFYKLLCDSPDYFSSQIEVNRSVQDVVALLRCSRYSLGIM 161

Query: 141 SFGNGLVMGWLRFSEAGRR-FDCIKKPNSAHSI--PVQVEEVTDIVSAAEYILIVEKESV 200
           +   GLV G L   E G+   DC    +S  +I   + + + T + + A YI+IVEK ++
Sbjct: 162 ASSRGLVAGRLFLQEPGKEAVDCSACGSSGFAITGDLNLLDNTIMRTDARYIIIVEKHAI 221

Query: 201 FQRLANDQFCSRNRCIVITGRGYPDVPTRRFL-RLLVDVSALPAFCLVDCDPYGFEILTT 260
           F RL  D+  +   C+ IT +GYPD+ TR FL R+      LP   LVD +P G  IL T
Sbjct: 222 FHRLVEDRVFNHIPCVFITAKGYPDIATRFFLHRMSTTFPDLPILVLVDWNPAGLAILCT 281

Query: 261 YRFGSMQMAYDAKYLRIPHLRWLGAFASDSENYNLPEQCLIPLTMEDKRRTEALLLRCYL 320
           ++FGS+ M  +A Y    +++W+G    D     +PE+ L+PL  +D +  ++LL    L
Sbjct: 282 FKFGSIGMGLEA-YRYACNVKWIGLRGDDLN--LIPEESLVPLKPKDSQIAKSLLSSKIL 341

Query: 321 QREVPHWRLELDSMLQKGVKFEIEALSVHNLSFLSEEYLPSKI 356
           Q    ++  EL  M+Q G + EIEAL  H  ++L  +Y+ +KI
Sbjct: 342 QE---NYIEELSLMVQTGKRAEIEALYCHGYNYLG-KYIATKI 377

The following BLAST results are available for this feature:

BLAST of MC11g1041 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M4A2	2.6e-144	69.01	Meiotic recombination protein SPO11-1 OS=Arabidopsis thaliana OX=3702 GN=SPO11-1...	[more]
Q5ZPV8	1.1e-126	59.33	Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. indica OX=39946 GN=...	[more]
Q7Y021	1.1e-126	59.33	Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q9Y5K1	1.8e-60	35.81	Meiotic recombination protein SPO11 OS=Homo sapiens OX=9606 GN=SPO11 PE=1 SV=1	[more]
Q9WTK8	3.4e-59	36.09	Meiotic recombination protein SPO11 OS=Mus musculus OX=10090 GN=Spo11 PE=1 SV=2	[more]

BLAST of MC11g1041 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139661.1	4.87e-266	100.00	meiotic recombination protein SPO11-1 [Momordica charantia]	[more]
XP_023536168.1	2.63e-234	89.58	meiotic recombination protein SPO11-1 [Cucurbita pepo subsp. pepo]	[more]
XP_022976331.1	6.50e-234	89.30	meiotic recombination protein SPO11-1 [Cucurbita maxima]	[more]
XP_022937001.1	1.57e-231	88.52	meiotic recombination protein SPO11-1 [Cucurbita moschata]	[more]
XP_008462989.1	1.20e-230	86.26	PREDICTED: meiotic recombination protein SPO11-1 isoform X1 [Cucumis melo]	[more]

BLAST of MC11g1041 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CDC7	2.36e-266	100.00	meiotic recombination protein SPO11-1 OS=Momordica charantia OX=3673 GN=LOC11101...	[more]
A0A6J1IGM0	3.15e-234	89.30	meiotic recombination protein SPO11-1 OS=Cucurbita maxima OX=3661 GN=LOC11147676...	[more]
A0A6J1F921	7.59e-232	88.52	meiotic recombination protein SPO11-1 OS=Cucurbita moschata OX=3662 GN=LOC111443...	[more]
A0A1S3CJR6	5.80e-231	86.26	meiotic recombination protein SPO11-1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A1S3CI64	2.61e-224	85.44	meiotic recombination protein SPO11-1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1...	[more]

BLAST of MC11g1041 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13170.1	1.9e-145	69.01	Spo11/DNA topoisomerase VI, subunit A protein	[more]
AT5G02820.1	3.5e-59	40.00	Spo11/DNA topoisomerase VI, subunit A protein	[more]
AT1G63990.1	4.9e-37	34.63	sporulation 11-2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013048	Meiotic recombination, Spo11	PRINTS	PR01551	SPO11HOMOLOG	coord: 246..265 score: 45.0 coord: 342..361 score: 30.0 coord: 104..124 score: 40.14 coord: 199..214 score: 27.68 coord: 281..305 score: 36.0
IPR002815	Spo11/DNA topoisomerase VI subunit A	PRINTS	PR01550	TOP6AFAMILY	coord: 112..129 score: 43.06 coord: 130..145 score: 41.02 coord: 241..257 score: 55.88 coord: 209..230 score: 46.88 coord: 98..109 score: 50.0 coord: 185..202 score: 54.51
IPR002815	Spo11/DNA topoisomerase VI subunit A	PANTHER	PTHR10848	MEIOTIC RECOMBINATION PROTEIN SPO11	coord: 4..356
None	No IPR available	GENE3D	3.40.1360.10		coord: 140..358 e-value: 1.8E-80 score: 271.7
None	No IPR available	PANTHER	PTHR10848:SF3	MEIOTIC RECOMBINATION PROTEIN SPO11-1	coord: 4..356
IPR013049	Spo11/DNA topoisomerase VI, subunit A, N-terminal	PFAM	PF04406	TP6A_N	coord: 74..135 e-value: 5.5E-21 score: 74.3
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 1..137 e-value: 3.0E-23 score: 84.2
IPR034136	Topoisomerase 6 subunit A/Spo11, TOPRIM domain	CDD	cd00223	TOPRIM_TopoIIB_SPO	coord: 183..345 e-value: 3.76344E-66 score: 204.796
IPR036078	Spo11/DNA topoisomerase VI subunit A superfamily	SUPERFAMILY	56726	DNA topoisomerase IV, alpha subunit	coord: 73..355

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g1041.1	MC11g1041.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051026	chiasma assembly
biological_process	GO:0000724	double-strand break repair via homologous recombination
biological_process	GO:0042138	meiotic DNA double-strand break formation
biological_process	GO:0006259	DNA metabolic process
cellular_component	GO:0005694	chromosome
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003918	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003824	catalytic activity