MC11g1026 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC11g1026
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionSerine O-acetyltransferase
LocationMC11: 8789088 .. 8790137 (+)
RNA-Seq ExpressionMC11g1026
SyntenyMC11g1026
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTCTATATAGACCCCTCTCTCGCCTCCACATGTCCTTCGTGAAGCCCCATTCCTTCAATTTCTCTGCAATTTCTCACCTCCAAGAACTCCCTCGCCCCTCTCCCATGGCCGCTTGCTTCGACACCACTCCCCTTCTCCCCCCCGATCCCCATTTCAAAATCCCCCAATTCTGCCGCTCCAATTTCTCCGATTCCGTCTCCTGCCTCCCTCTTCCCCCAAAATTTGATTCCGATTCCGATTTCGATCTCTGGCTCAAAATCCGCGACGAAGCGTCCGCCGATGTCGAGCACGAACCGATTCTCTCCAACTACTACATCGATTCGATTCTCTCCCACGCCTCCATGGACGCCGCGCTCGCGAATCACCTCTCGATTAAGCTCTGCAACTCAGCGCTCCCGAGCCCTACTCTGTTCGAGCTCTTCTCCGAGGTGGTTAGGGCGGACGGCGGAGAGATTCTGCGGTGCGCGGCGAGGGATTTGCGGGCCGTCAAGGAACGGGACCCGGCTTGTATTAGCTTCGTTCAGTGCTTTTTGAATTACAAAGGGTTTCTCGCGGTTCAATCGCATCGAATCGCGAATAAGCTCTGGTCTCAGGGGAGGAAAGTTCTCGCGATTGTGATTCAGAACAGAGTATCGGAGGTTTTCGCCGTCGATATCCACCCCGGCGCGAAGATCGGCTGCGGGATTCTGCTCGACCACGCCACCGGTGTCGTCGTCGGCGAGACGGCGGTGATCGGAAACGACGTGTCGATTCTCCACCACGTGACGTTGGGGGGAACCGGGAAGGACTCCGGCGACCGGCACCCGAAGATTGGGGACGGCGTGCTGATCGGCGCCGGAACTTGCATTTTGGGGAACATTAATATCGGCGACGGCGCGAAGATCGGAGCTGGGTCGTTGGTGTTGAAGGACGTACCGCCGCGCACGACGGCGGTGGGGAATCCGGCGAGGCTGGTCGGCGGGAAGGCGAATCCGATCAAGCTGGATAAAACGCCGAGTTTCACCATGGATCATACTTCTCATATAAGTGAGTGGTCTGATTATGTTATA

mRNA sequence

CCTCTATATAGACCCCTCTCTCGCCTCCACATGTCCTTCGTGAAGCCCCATTCCTTCAATTTCTCTGCAATTTCTCACCTCCAAGAACTCCCTCGCCCCTCTCCCATGGCCGCTTGCTTCGACACCACTCCCCTTCTCCCCCCCGATCCCCATTTCAAAATCCCCCAATTCTGCCGCTCCAATTTCTCCGATTCCGTCTCCTGCCTCCCTCTTCCCCCAAAATTTGATTCCGATTCCGATTTCGATCTCTGGCTCAAAATCCGCGACGAAGCGTCCGCCGATGTCGAGCACGAACCGATTCTCTCCAACTACTACATCGATTCGATTCTCTCCCACGCCTCCATGGACGCCGCGCTCGCGAATCACCTCTCGATTAAGCTCTGCAACTCAGCGCTCCCGAGCCCTACTCTGTTCGAGCTCTTCTCCGAGGTGGTTAGGGCGGACGGCGGAGAGATTCTGCGGTGCGCGGCGAGGGATTTGCGGGCCGTCAAGGAACGGGACCCGGCTTGTATTAGCTTCGTTCAGTGCTTTTTGAATTACAAAGGGTTTCTCGCGGTTCAATCGCATCGAATCGCGAATAAGCTCTGGTCTCAGGGGAGGAAAGTTCTCGCGATTGTGATTCAGAACAGAGTATCGGAGGTTTTCGCCGTCGATATCCACCCCGGCGCGAAGATCGGCTGCGGGATTCTGCTCGACCACGCCACCGGTGTCGTCGTCGGCGAGACGGCGGTGATCGGAAACGACGTGTCGATTCTCCACCACGTGACGTTGGGGGGAACCGGGAAGGACTCCGGCGACCGGCACCCGAAGATTGGGGACGGCGTGCTGATCGGCGCCGGAACTTGCATTTTGGGGAACATTAATATCGGCGACGGCGCGAAGATCGGAGCTGGGTCGTTGGTGTTGAAGGACGTACCGCCGCGCACGACGGCGGTGGGGAATCCGGCGAGGCTGGTCGGCGGGAAGGCGAATCCGATCAAGCTGGATAAAACGCCGAGTTTCACCATGGATCATACTTCTCATATAAGTGAGTGGTCTGATTATGTTATA

Coding sequence (CDS)

CCTCTATATAGACCCCTCTCTCGCCTCCACATGTCCTTCGTGAAGCCCCATTCCTTCAATTTCTCTGCAATTTCTCACCTCCAAGAACTCCCTCGCCCCTCTCCCATGGCCGCTTGCTTCGACACCACTCCCCTTCTCCCCCCCGATCCCCATTTCAAAATCCCCCAATTCTGCCGCTCCAATTTCTCCGATTCCGTCTCCTGCCTCCCTCTTCCCCCAAAATTTGATTCCGATTCCGATTTCGATCTCTGGCTCAAAATCCGCGACGAAGCGTCCGCCGATGTCGAGCACGAACCGATTCTCTCCAACTACTACATCGATTCGATTCTCTCCCACGCCTCCATGGACGCCGCGCTCGCGAATCACCTCTCGATTAAGCTCTGCAACTCAGCGCTCCCGAGCCCTACTCTGTTCGAGCTCTTCTCCGAGGTGGTTAGGGCGGACGGCGGAGAGATTCTGCGGTGCGCGGCGAGGGATTTGCGGGCCGTCAAGGAACGGGACCCGGCTTGTATTAGCTTCGTTCAGTGCTTTTTGAATTACAAAGGGTTTCTCGCGGTTCAATCGCATCGAATCGCGAATAAGCTCTGGTCTCAGGGGAGGAAAGTTCTCGCGATTGTGATTCAGAACAGAGTATCGGAGGTTTTCGCCGTCGATATCCACCCCGGCGCGAAGATCGGCTGCGGGATTCTGCTCGACCACGCCACCGGTGTCGTCGTCGGCGAGACGGCGGTGATCGGAAACGACGTGTCGATTCTCCACCACGTGACGTTGGGGGGAACCGGGAAGGACTCCGGCGACCGGCACCCGAAGATTGGGGACGGCGTGCTGATCGGCGCCGGAACTTGCATTTTGGGGAACATTAATATCGGCGACGGCGCGAAGATCGGAGCTGGGTCGTTGGTGTTGAAGGACGTACCGCCGCGCACGACGGCGGTGGGGAATCCGGCGAGGCTGGTCGGCGGGAAGGCGAATCCGATCAAGCTGGATAAAACGCCGAGTTTCACCATGGATCATACTTCTCATATAAGTGAGTGGTCTGATTATGTTATA

Protein sequence

PLYRPLSRLHMSFVKPHSFNFSAISHLQELPRPSPMAACFDTTPLLPPDPHFKIPQFCRSNFSDSVSCLPLPPKFDSDSDFDLWLKIRDEASADVEHEPILSNYYIDSILSHASMDAALANHLSIKLCNSALPSPTLFELFSEVVRADGGEILRCAARDLRAVKERDPACISFVQCFLNYKGFLAVQSHRIANKLWSQGRKVLAIVIQNRVSEVFAVDIHPGAKIGCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTGKDSGDRHPKIGDGVLIGAGTCILGNINIGDGAKIGAGSLVLKDVPPRTTAVGNPARLVGGKANPIKLDKTPSFTMDHTSHISEWSDYVI
Homology
BLAST of MC11g1026 vs. ExPASy Swiss-Prot
Match: Q39218 (Serine acetyltransferase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SAT3 PE=1 SV=3)

HSP 1 Score: 403.7 bits (1036), Expect = 2.2e-111
Identity = 220/369 (59.62%), Postives = 264/369 (71.54%), Query Frame = 0

Query: 10  HMSFVKP----HSFNFSAISHLQELPRPSPMAACFDT----TPLLPPDP----------- 69
           H SF+ P      F    +S     P P PMAAC DT     P + P             
Sbjct: 26  HHSFLLPSFVSSKFKHHTLSPPPSPPPPPPMAACIDTCRTGKPQISPRDSSKHHDDESGF 85

Query: 70  ----HFKIPQFCRSNF----SDSVSCLPLPPKFDSDSDF-DLWLKIRDEASADVEHEPIL 129
               +F+ P   RS+F    + ++   PL    D D++  D+W KIR+EA +D+  EPI+
Sbjct: 86  RYMNYFRYPD--RSSFNGTQTKTLHTRPLLEDLDRDAEVDDVWAKIREEAKSDIAKEPIV 145

Query: 130 SNYYIDSILSHASMDAALANHLSIKLCNSALPSPTLFELFSEVVRADGGEILRCAARDLR 189
           S YY  SI+S  S++AALAN LS+KL N  LPS TLF+LFS V++ +  +I+     DL 
Sbjct: 146 SAYYHASIVSQRSLEAALANTLSVKLSNLNLPSNTLFDLFSGVLQGN-PDIVESVKLDLL 205

Query: 190 AVKERDPACISFVQCFLNYKGFLAVQSHRIANKLWSQGRKVLAIVIQNRVSEVFAVDIHP 249
           AVKERDPACIS+V CFL++KGFLA Q+HRIA++LW+Q RK+LA++IQNRVSE FAVD HP
Sbjct: 206 AVKERDPACISYVHCFLHFKGFLACQAHRIAHELWTQDRKILALLIQNRVSEAFAVDFHP 265

Query: 250 GAKIGCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTGKDSGDRHPKIGDGVLIGAGT 309
           GAKIG GILLDHAT +V+GETAV+GN+VSILH+VTLGGTGK  GDRHPKIGDGVLIGAGT
Sbjct: 266 GAKIGTGILLDHATAIVIGETAVVGNNVSILHNVTLGGTGKQCGDRHPKIGDGVLIGAGT 325

Query: 310 CILGNINIGDGAKIGAGSLVLKDVPPRTTAVGNPARLVGGKANPIKLDKTPSFTMDHTSH 351
           CILGNI IG+GAKIGAGS+VLKDVPPRTTAVGNPARL+GGK NP   DK P  TMD TSH
Sbjct: 326 CILGNITIGEGAKIGAGSVVLKDVPPRTTAVGNPARLLGGKDNPKTHDKIPGLTMDQTSH 385

BLAST of MC11g1026 vs. ExPASy Swiss-Prot
Match: Q42588 (Serine acetyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SAT1 PE=1 SV=2)

HSP 1 Score: 388.3 bits (996), Expect = 9.4e-107
Identity = 192/273 (70.33%), Postives = 229/273 (83.88%), Query Frame = 0

Query: 78  DSDFDLWLKIRDEASADVEHEPILSNYYIDSILSHASMDAALANHLSIKLCNSALPSPTL 137
           + D D+W+K+ +EA +DV+ EPILSNYY  SI SH S+++ALA+ LS+KL N  LPS TL
Sbjct: 43  EDDDDVWIKMLEEAKSDVKQEPILSNYYYASITSHRSLESALAHILSVKLSNLNLPSNTL 102

Query: 138 FELFSEVVRADGGEILRCAARDLRAVKERDPACISFVQCFLNYKGFLAVQSHRIANKLWS 197
           FELF  V+  +  EI+    +DL AVKERDPACIS+V CFL +KGFLA Q+HRIA+ LW 
Sbjct: 103 FELFISVLE-ESPEIIESTKQDLIAVKERDPACISYVHCFLGFKGFLACQAHRIAHTLWK 162

Query: 198 QGRKVLAIVIQNRVSEVFAVDIHPGAKIGCGILLDHATGVVVGETAVIGNDVSILHHVTL 257
           Q RK++A++IQNRVSE FAVDIHPGAKIG GILLDHATGVV+GETAV+G++VSILH VTL
Sbjct: 163 QNRKIVALLIQNRVSESFAVDIHPGAKIGKGILLDHATGVVIGETAVVGDNVSILHGVTL 222

Query: 258 GGTGKDSGDRHPKIGDGVLIGAGTCILGNINIGDGAKIGAGSLVLKDVPPRTTAVGNPAR 317
           GGTGK SGDRHPKIGDGVLIGAG+CILGNI IG+GAKIG+GS+V+KDVP RTTAVGNPAR
Sbjct: 223 GGTGKQSGDRHPKIGDGVLIGAGSCILGNITIGEGAKIGSGSVVVKDVPARTTAVGNPAR 282

Query: 318 LVGGKANPIKLDKTPSFTMDHTSHISEWSDYVI 351
           L+GGK NP K DK P  TMD TS+++EWSDYVI
Sbjct: 283 LIGGKENPRKHDKIPCLTMDQTSYLTEWSDYVI 314

BLAST of MC11g1026 vs. ExPASy Swiss-Prot
Match: Q42538 (Serine acetyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SAT5 PE=1 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 3.8e-92
Identity = 171/269 (63.57%), Postives = 207/269 (76.95%), Query Frame = 0

Query: 83  LWLKIRDEASADVEHEPILSNYYIDSILSHASMDAALANHLSIKLCNSALPSPTLFELFS 142
           LW +I+ EA  D E EP L++Y   +ILSH+S++ +++ HL  KLC+S L S  L++LF 
Sbjct: 45  LWTQIKAEARRDAEAEPALASYLYSTILSHSSLERSISFHLGNKLCSSTLLSTLLYDLFL 104

Query: 143 EVVRADGGEILRCAARDLRAVKERDPACISFVQCFLNYKGFLAVQSHRIANKLWSQGRKV 202
               +D   +      DLRA + RDPACISF  C LNYKGFLA+Q+HR+++KLW+Q RK 
Sbjct: 105 NTFSSD-PSLRNATVADLRAARVRDPACISFSHCLLNYKGFLAIQAHRVSHKLWTQSRKP 164

Query: 203 LAIVIQNRVSEVFAVDIHPGAKIGCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTGK 262
           LA+ + +R+S+VFAVDIHP AKIG GILLDHATGVVVGETAVIGN+VSILHHVTLGGTGK
Sbjct: 165 LALALHSRISDVFAVDIHPAAKIGKGILLDHATGVVVGETAVIGNNVSILHHVTLGGTGK 224

Query: 263 DSGDRHPKIGDGVLIGAGTCILGNINIGDGAKIGAGSLVLKDVPPRTTAVGNPARLVGGK 322
             GDRHPKIGDG LIGAG  ILGN+ IG GAK+GAGS+VL DVP R TAVGNPARLVGGK
Sbjct: 225 ACGDRHPKIGDGCLIGAGATILGNVKIGAGAKVGAGSVVLIDVPCRGTAVGNPARLVGGK 284

Query: 323 ANP-IKLDKTPSFTMDHTSHISEWSDYVI 351
             P I  ++ P  +MDHTS ISEWSDY+I
Sbjct: 285 EKPTIHDEECPGESMDHTSFISEWSDYII 312

BLAST of MC11g1026 vs. ExPASy Swiss-Prot
Match: Q8W0E4 (Probable serine acetyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=SAT1 PE=2 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 2.3e-89
Identity = 172/280 (61.43%), Postives = 210/280 (75.00%), Query Frame = 0

Query: 72  PPKFDSDSDFDLWLKIRDEASADVEHEPILSNYYIDSILSHASMDAALANHLSIKLCNSA 131
           PP+ D+D  + +W +I+ EA  D + EP L+++   ++LSH S+D +LA HL+ KLC+S 
Sbjct: 31  PPESDADESW-VWSQIKAEARRDADAEPALASFLYATVLSHPSLDRSLAFHLANKLCSST 90

Query: 132 LPSPTLFELFSEVVRADGGEILRCA-ARDLRAVKERDPACISFVQCFLNYKGFLAVQSHR 191
           L S  L++LF  V        LR A   DL A + RDPAC+ F  C LNYKGFLA+Q+ R
Sbjct: 91  LLSTLLYDLF--VASLAAHPTLRAAVVADLLAARSRDPACVGFSHCLLNYKGFLAIQAQR 150

Query: 192 IANKLWSQGRKVLAIVIQNRVSEVFAVDIHPGAKIGCGILLDHATGVVVGETAVIGNDVS 251
           +A+ LW+Q R+ LA+ +Q+RV+EVFAVDIHP A IG G+LLDHATGVV+GETAVIG++VS
Sbjct: 151 VAHVLWAQDRRALALALQSRVAEVFAVDIHPAAAIGKGVLLDHATGVVIGETAVIGDNVS 210

Query: 252 ILHHVTLGGTGKDSGDRHPKIGDGVLIGAGTCILGNINIGDGAKIGAGSLVLKDVPPRTT 311
           ILHHVTLGGTGK  GDRHPKIGDGVLIGAG  ILGN+ IG GAKIGAGSLVL DVPPRTT
Sbjct: 211 ILHHVTLGGTGKAVGDRHPKIGDGVLIGAGATILGNVRIGAGAKIGAGSLVLIDVPPRTT 270

Query: 312 AVGNPARLVGGKANPIKLDKTPSFTMDHTSHISEWSDYVI 351
           AVGNPARL+GGK    K D  P  +MDHTS I +WSDY I
Sbjct: 271 AVGNPARLLGGK----KGDDMPGESMDHTSFIQQWSDYSI 303

BLAST of MC11g1026 vs. ExPASy Swiss-Prot
Match: Q0DGG8 (Probable serine acetyltransferase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=SAT5 PE=2 SV=2)

HSP 1 Score: 313.9 bits (803), Expect = 2.3e-84
Identity = 160/281 (56.94%), Postives = 209/281 (74.38%), Query Frame = 0

Query: 72  PPKFDSDSDFDLWLKIRDEASADVEHEPILSNYYIDSILSHASMDAALANHLSIKLCNSA 131
           PP+ + +  + +W +I+ EA  D + EP L+++   ++LSH S+  +++ HL+ KLC+S 
Sbjct: 63  PPESEDEESW-VWSQIKAEARRDADAEPALASFLYATVLSHPSLPRSISFHLANKLCSST 122

Query: 132 LPSPTLFELFSEVVRADGGEILRCA-ARDLRAVKERDPACISFVQCFLNYKGFLAVQSHR 191
           L S  L++LF     A     LR A   DL A + RDPAC+ F QC LN+KGFLA+Q+HR
Sbjct: 123 LLSTLLYDLFLASFTAHPS--LRAAVVADLLAARSRDPACVGFSQCLLNFKGFLAIQAHR 182

Query: 192 IANKLWSQGRKVLAIVIQNRVSEVFAVDIHPGAKIGCGILLDHATGVVVGETAVIGNDVS 251
           +++ LW+Q R+ LA+ +Q+RV++VFAVDIHP A +G GILLDHATGVV+GETAV+G++VS
Sbjct: 183 VSHVLWAQQRRPLALALQSRVADVFAVDIHPAAVVGKGILLDHATGVVIGETAVVGDNVS 242

Query: 252 ILHHVTLGGTGKDSGDRHPKIGDGVLIGAGTCILGNINIGDGAKIGAGSLVLKDVPPRTT 311
           ILHHVTLGGTGK  GDRHPKIGDGVLIGAG  ILGN+ IG GAKIGAGS+VL DVP R T
Sbjct: 243 ILHHVTLGGTGKAVGDRHPKIGDGVLIGAGATILGNVKIGAGAKIGAGSVVLIDVPARNT 302

Query: 312 AVGNPARLVGGKANPIKLDK-TPSFTMDHTSHISEWSDYVI 351
           AVGNPARL+G K   ++ D+  P  +MDHTS I +WSDY I
Sbjct: 303 AVGNPARLIGRKNGEVEKDEDMPGESMDHTSFIRQWSDYTI 340

BLAST of MC11g1026 vs. NCBI nr
Match: XP_038900115.1 (serine acetyltransferase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 534 bits (1375), Expect = 2.00e-188
Identity = 278/359 (77.44%), Postives = 298/359 (83.01%), Query Frame = 0

Query: 1   PLYRPLSRLHMSFVKPHSFNFSAISHLQELPRPSPMAACFDTTPLLP-PDPHFKIPQFCR 60
           PLYRP+SR HMSF+   S  FS    L     PSPMAA     PL   P+   KI +FCR
Sbjct: 4   PLYRPVSRFHMSFISHFSSLFSPFPDL----HPSPMAAA--CLPLSSEPNSSLKILKFCR 63

Query: 61  SNFSDSVSCLPLPPK--------FDSDSDFDLWLKIRDEASADVEHEPILSNYYIDSILS 120
             FSD +S LP+ P              D DLW  IR EA +DVE EPILSNYYI SILS
Sbjct: 64  PTFSDHLSSLPISPNPQKTHDSPLSPSPDSDLWFLIRQEARSDVEDEPILSNYYITSILS 123

Query: 121 HASMDAALANHLSIKLCNSALPSPTLFELFSEVVRADGGEILRCAARDLRAVKERDPACI 180
           HASM+ ALANHLSIKLCNS+LPS TLF+LFS+V+  DGG+ILRCA RDL+AVKERDPACI
Sbjct: 124 HASMEKALANHLSIKLCNSSLPSSTLFQLFSDVLTDDGGDILRCAIRDLKAVKERDPACI 183

Query: 181 SFVQCFLNYKGFLAVQSHRIANKLWSQGRKVLAIVIQNRVSEVFAVDIHPGAKIGCGILL 240
           S+VQCFLNYKGFLAVQSHRIA+KLW+QGRKVLA++IQNRVSE FAVDIHPGAKIGCGILL
Sbjct: 184 SYVQCFLNYKGFLAVQSHRIAHKLWNQGRKVLAMLIQNRVSEAFAVDIHPGAKIGCGILL 243

Query: 241 DHATGVVVGETAVIGNDVSILHHVTLGGTGKDSGDRHPKIGDGVLIGAGTCILGNINIGD 300
           DHATGVVVGETAVIGNDVSILHHVTLGGTGKDSGDRHPKIGDGVLIGAGTCILGNI IGD
Sbjct: 244 DHATGVVVGETAVIGNDVSILHHVTLGGTGKDSGDRHPKIGDGVLIGAGTCILGNIKIGD 303

Query: 301 GAKIGAGSLVLKDVPPRTTAVGNPARLVGGKANPIKLDKTPSFTMDHTSHISEWSDYVI 350
           GAKIGAGS+VLKDVPPRTTAVGNPARLVGGK NPIKLDKTPSFTMDHTSHISEWSDYVI
Sbjct: 304 GAKIGAGSVVLKDVPPRTTAVGNPARLVGGKVNPIKLDKTPSFTMDHTSHISEWSDYVI 356

BLAST of MC11g1026 vs. NCBI nr
Match: XP_004150470.2 (serine acetyltransferase 1, chloroplastic [Cucumis sativus] >KAE8645986.1 hypothetical protein Csa_015472 [Cucumis sativus])

HSP 1 Score: 532 bits (1371), Expect = 7.28e-188
Identity = 277/360 (76.94%), Postives = 297/360 (82.50%), Query Frame = 0

Query: 1   PLYRPLSRLHMSFVKPHSFNFSAISHLQELPRPSPMAACFDTTPLLPPDPH--FKIPQFC 60
           PLYRPLSRLHMSF+   S  FS    L   P     AAC        PDP+   KI +FC
Sbjct: 4   PLYRPLSRLHMSFIPHFSSLFSPFPDLHLSPM---AAACL-------PDPNSSLKILKFC 63

Query: 61  RSNFSDSVSCLPLPPK--------FDSDSDFDLWLKIRDEASADVEHEPILSNYYIDSIL 120
           R  FSD +S L + P             SD DLW  IR EA +DVEHEPILSNYYI SIL
Sbjct: 64  RPTFSDHLSTLSISPNPQNTHDSSLSPSSDSDLWFLIRQEACSDVEHEPILSNYYITSIL 123

Query: 121 SHASMDAALANHLSIKLCNSALPSPTLFELFSEVVRADGGEILRCAARDLRAVKERDPAC 180
           +HASMD ALANHLSIKL NS+LPS TLF+LFS+V+  DGGEILRCA RDL+AVKERDPAC
Sbjct: 124 THASMDKALANHLSIKLSNSSLPSSTLFQLFSDVLTDDGGEILRCAVRDLKAVKERDPAC 183

Query: 181 ISFVQCFLNYKGFLAVQSHRIANKLWSQGRKVLAIVIQNRVSEVFAVDIHPGAKIGCGIL 240
           IS+VQCFLNYKGFLAVQSHRIA+KLWS+GRKVLA++IQNRVSE FAVDIHPGAKIGCGIL
Sbjct: 184 ISYVQCFLNYKGFLAVQSHRIAHKLWSEGRKVLAMLIQNRVSEAFAVDIHPGAKIGCGIL 243

Query: 241 LDHATGVVVGETAVIGNDVSILHHVTLGGTGKDSGDRHPKIGDGVLIGAGTCILGNINIG 300
           LDHATGVVVGETAVIGNDVSILHHVTLGGTGKD+GDRHPKIGDGVLIGAGTCILGNI IG
Sbjct: 244 LDHATGVVVGETAVIGNDVSILHHVTLGGTGKDTGDRHPKIGDGVLIGAGTCILGNIKIG 303

Query: 301 DGAKIGAGSLVLKDVPPRTTAVGNPARLVGGKANPIKLDKTPSFTMDHTSHISEWSDYVI 350
           DGAKIGAGS+VLKDVPPRTTAVGNPARL+GGK NPIKLDKTPSFTMDHTSHISEWSDYVI
Sbjct: 304 DGAKIGAGSVVLKDVPPRTTAVGNPARLIGGKVNPIKLDKTPSFTMDHTSHISEWSDYVI 353

BLAST of MC11g1026 vs. NCBI nr
Match: KAG6607765.1 (Serine acetyltransferase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 531 bits (1367), Expect = 5.68e-187
Identity = 274/365 (75.07%), Postives = 305/365 (83.56%), Query Frame = 0

Query: 1   PLYRPLSRLHMSFVKPHSFNFSA-ISHLQELPRPSPMAAC----------FDTTPLLPPD 60
           PLYRPLSRL M+   P  F+ S+ ISH  +L  PSPMAA           FD T  LP D
Sbjct: 8   PLYRPLSRLPMALHNPSPFHLSSLISHSPDL-HPSPMAAACLHHNSPPLHFDPTTPLPTD 67

Query: 61  PHFKIPQFCRSNFSDSVSCLPLPPK----FDSDSDFDLWLKIRDEASADVEHEPILSNYY 120
           PH KI  F R  FSD +S  P+ P        DSD DLW KI++EA AD+EHEPIL++YY
Sbjct: 68  PHLKILNFSRPTFSDHLSPPPISPNPQILNSPDSDSDLWFKIQEEARADMEHEPILASYY 127

Query: 121 IDSILSHASMDAALANHLSIKLCNSALPSPTLFELFSEVVRADGGEILRCAARDLRAVKE 180
           I SILSH SM+ ALANHLSIKLCN +LPS TLFELF++V++ D GEILRC+ RDL+AVKE
Sbjct: 128 ITSILSHKSMERALANHLSIKLCNLSLPSSTLFELFTDVIQEDAGEILRCSIRDLQAVKE 187

Query: 181 RDPACISFVQCFLNYKGFLAVQSHRIANKLWSQGRKVLAIVIQNRVSEVFAVDIHPGAKI 240
           RDPA IS+VQCFLNYKGFLAVQSHRIA+KLW +GRKVLA++IQNRV+E FAVDIHPGA+I
Sbjct: 188 RDPAWISYVQCFLNYKGFLAVQSHRIAHKLWCRGRKVLALLIQNRVAEAFAVDIHPGARI 247

Query: 241 GCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTGKDSGDRHPKIGDGVLIGAGTCILG 300
           GCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTGK+SGDRHPKIGDGVLIGAGTCILG
Sbjct: 248 GCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTGKNSGDRHPKIGDGVLIGAGTCILG 307

Query: 301 NINIGDGAKIGAGSLVLKDVPPRTTAVGNPARLVGGKANPIKLDKTPSFTMDHTSHISEW 350
           NI IGDGAKIGAGS+VLKDVPPRTTAVGNPARL+GGKANPIKLDKTPSFTMDHTSHI++W
Sbjct: 308 NIKIGDGAKIGAGSVVLKDVPPRTTAVGNPARLIGGKANPIKLDKTPSFTMDHTSHINDW 367

BLAST of MC11g1026 vs. NCBI nr
Match: XP_008462963.1 (PREDICTED: serine acetyltransferase 1, chloroplastic [Cucumis melo])

HSP 1 Score: 528 bits (1360), Expect = 3.84e-186
Identity = 276/358 (77.09%), Postives = 297/358 (82.96%), Query Frame = 0

Query: 1   PLYRPLSRLHMSFVKPHSFNFSAISHLQELPRPSPMAACFDTTPLLPPDPHFKIPQFCRS 60
           PLYRPLSRLHMSF+   S  FS    L   P     AAC   +    P+   KI +FCR 
Sbjct: 4   PLYRPLSRLHMSFIPHFSSLFSPFPDLHLSPM---AAACLPLSS--DPNSSLKILKFCRP 63

Query: 61  NFSDSVSCLPLPPK----FDS----DSDFDLWLKIRDEASADVEHEPILSNYYIDSILSH 120
            FSD +S L + P      DS     SD DLW  IR EA +DVE EPILSNYYI SIL+H
Sbjct: 64  TFSDHLSSLSISPNPQNTHDSLLPPSSDSDLWFLIRQEACSDVEQEPILSNYYITSILTH 123

Query: 121 ASMDAALANHLSIKLCNSALPSPTLFELFSEVVRADGGEILRCAARDLRAVKERDPACIS 180
           ASMD ALANHLSIKL NS+LPS TLF+LFS+V+  DGGEILRCA RDL+AVKERDPACIS
Sbjct: 124 ASMDKALANHLSIKLSNSSLPSSTLFQLFSDVLTDDGGEILRCAVRDLKAVKERDPACIS 183

Query: 181 FVQCFLNYKGFLAVQSHRIANKLWSQGRKVLAIVIQNRVSEVFAVDIHPGAKIGCGILLD 240
           +VQCFLNYKGFLAVQSHRIA+KLWS+GRKVLA++IQNRVSE FAVDIHPGAKIGCGILLD
Sbjct: 184 YVQCFLNYKGFLAVQSHRIAHKLWSEGRKVLAMLIQNRVSEAFAVDIHPGAKIGCGILLD 243

Query: 241 HATGVVVGETAVIGNDVSILHHVTLGGTGKDSGDRHPKIGDGVLIGAGTCILGNINIGDG 300
           HATGVVVGETAVIGNDVSILHHVTLGGTGKDSGDRHPKIGDGVLIGAGTCILGNI IGDG
Sbjct: 244 HATGVVVGETAVIGNDVSILHHVTLGGTGKDSGDRHPKIGDGVLIGAGTCILGNIKIGDG 303

Query: 301 AKIGAGSLVLKDVPPRTTAVGNPARLVGGKANPIKLDKTPSFTMDHTSHISEWSDYVI 350
           AKIGAGS+VLKDVPPRTTAVGNPARL+GGK NPIKLDKTPSFTMDHTSHI+EWSDYVI
Sbjct: 304 AKIGAGSVVLKDVPPRTTAVGNPARLIGGKVNPIKLDKTPSFTMDHTSHINEWSDYVI 356

BLAST of MC11g1026 vs. NCBI nr
Match: XP_022940668.1 (serine acetyltransferase 1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 527 bits (1358), Expect = 1.33e-185
Identity = 274/365 (75.07%), Postives = 306/365 (83.84%), Query Frame = 0

Query: 1   PLYRPLSRLHMSFVKPHSFNFSA-ISHLQELPRPSPMAAC----------FDTTPLLPPD 60
           PLYRPLSRL M+   P  F+ S+ ISH  +L  PSPMAA           FD T  LP D
Sbjct: 8   PLYRPLSRLPMALHNPSPFHLSSLISHSPDL-HPSPMAAACLHHNSPPLHFDPTTPLPTD 67

Query: 61  PHFKIPQFCRSNFSDSVSCLPLPPK---FDSD-SDFDLWLKIRDEASADVEHEPILSNYY 120
           PH KI  F R  FSD +S  P+ P     +S  SD DLW KI++EA AD+EHEPIL++YY
Sbjct: 68  PHLKILNFSRPTFSDHLSPPPISPNPQILNSPVSDSDLWFKIQEEARADMEHEPILASYY 127

Query: 121 IDSILSHASMDAALANHLSIKLCNSALPSPTLFELFSEVVRADGGEILRCAARDLRAVKE 180
           I SILSH SM+ ALANHLSIKLCN +LPS TLFELF++V++ D GEILRC+ RDL+AVKE
Sbjct: 128 ITSILSHKSMERALANHLSIKLCNLSLPSSTLFELFTDVIQEDAGEILRCSIRDLQAVKE 187

Query: 181 RDPACISFVQCFLNYKGFLAVQSHRIANKLWSQGRKVLAIVIQNRVSEVFAVDIHPGAKI 240
           RDPA IS+VQCFLNYKGFLAVQSHRIA+KLW +GRKVLA++IQNRV+E FAVDIHPGA+I
Sbjct: 188 RDPAWISYVQCFLNYKGFLAVQSHRIAHKLWCRGRKVLALLIQNRVAEAFAVDIHPGARI 247

Query: 241 GCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTGKDSGDRHPKIGDGVLIGAGTCILG 300
           GCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTGK+SGDRHPKIGDGVLIGAGTCILG
Sbjct: 248 GCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTGKNSGDRHPKIGDGVLIGAGTCILG 307

Query: 301 NINIGDGAKIGAGSLVLKDVPPRTTAVGNPARLVGGKANPIKLDKTPSFTMDHTSHISEW 350
           NI IGDGAKIGAGS+VLKDVPPRTTAVGNPARL+GGKANPIKLDKTPSFTMDHTSHI++W
Sbjct: 308 NIKIGDGAKIGAGSVVLKDVPPRTTAVGNPARLIGGKANPIKLDKTPSFTMDHTSHINDW 367

BLAST of MC11g1026 vs. ExPASy TrEMBL
Match: A0A1S3CI27 (Serine O-acetyltransferase OS=Cucumis melo OX=3656 GN=LOC103501225 PE=3 SV=1)

HSP 1 Score: 528 bits (1360), Expect = 1.86e-186
Identity = 276/358 (77.09%), Postives = 297/358 (82.96%), Query Frame = 0

Query: 1   PLYRPLSRLHMSFVKPHSFNFSAISHLQELPRPSPMAACFDTTPLLPPDPHFKIPQFCRS 60
           PLYRPLSRLHMSF+   S  FS    L   P     AAC   +    P+   KI +FCR 
Sbjct: 4   PLYRPLSRLHMSFIPHFSSLFSPFPDLHLSPM---AAACLPLSS--DPNSSLKILKFCRP 63

Query: 61  NFSDSVSCLPLPPK----FDS----DSDFDLWLKIRDEASADVEHEPILSNYYIDSILSH 120
            FSD +S L + P      DS     SD DLW  IR EA +DVE EPILSNYYI SIL+H
Sbjct: 64  TFSDHLSSLSISPNPQNTHDSLLPPSSDSDLWFLIRQEACSDVEQEPILSNYYITSILTH 123

Query: 121 ASMDAALANHLSIKLCNSALPSPTLFELFSEVVRADGGEILRCAARDLRAVKERDPACIS 180
           ASMD ALANHLSIKL NS+LPS TLF+LFS+V+  DGGEILRCA RDL+AVKERDPACIS
Sbjct: 124 ASMDKALANHLSIKLSNSSLPSSTLFQLFSDVLTDDGGEILRCAVRDLKAVKERDPACIS 183

Query: 181 FVQCFLNYKGFLAVQSHRIANKLWSQGRKVLAIVIQNRVSEVFAVDIHPGAKIGCGILLD 240
           +VQCFLNYKGFLAVQSHRIA+KLWS+GRKVLA++IQNRVSE FAVDIHPGAKIGCGILLD
Sbjct: 184 YVQCFLNYKGFLAVQSHRIAHKLWSEGRKVLAMLIQNRVSEAFAVDIHPGAKIGCGILLD 243

Query: 241 HATGVVVGETAVIGNDVSILHHVTLGGTGKDSGDRHPKIGDGVLIGAGTCILGNINIGDG 300
           HATGVVVGETAVIGNDVSILHHVTLGGTGKDSGDRHPKIGDGVLIGAGTCILGNI IGDG
Sbjct: 244 HATGVVVGETAVIGNDVSILHHVTLGGTGKDSGDRHPKIGDGVLIGAGTCILGNIKIGDG 303

Query: 301 AKIGAGSLVLKDVPPRTTAVGNPARLVGGKANPIKLDKTPSFTMDHTSHISEWSDYVI 350
           AKIGAGS+VLKDVPPRTTAVGNPARL+GGK NPIKLDKTPSFTMDHTSHI+EWSDYVI
Sbjct: 304 AKIGAGSVVLKDVPPRTTAVGNPARLIGGKVNPIKLDKTPSFTMDHTSHINEWSDYVI 356

BLAST of MC11g1026 vs. ExPASy TrEMBL
Match: A0A6J1FPY3 (Serine O-acetyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446191 PE=3 SV=1)

HSP 1 Score: 527 bits (1358), Expect = 6.44e-186
Identity = 274/365 (75.07%), Postives = 306/365 (83.84%), Query Frame = 0

Query: 1   PLYRPLSRLHMSFVKPHSFNFSA-ISHLQELPRPSPMAAC----------FDTTPLLPPD 60
           PLYRPLSRL M+   P  F+ S+ ISH  +L  PSPMAA           FD T  LP D
Sbjct: 8   PLYRPLSRLPMALHNPSPFHLSSLISHSPDL-HPSPMAAACLHHNSPPLHFDPTTPLPTD 67

Query: 61  PHFKIPQFCRSNFSDSVSCLPLPPK---FDSD-SDFDLWLKIRDEASADVEHEPILSNYY 120
           PH KI  F R  FSD +S  P+ P     +S  SD DLW KI++EA AD+EHEPIL++YY
Sbjct: 68  PHLKILNFSRPTFSDHLSPPPISPNPQILNSPVSDSDLWFKIQEEARADMEHEPILASYY 127

Query: 121 IDSILSHASMDAALANHLSIKLCNSALPSPTLFELFSEVVRADGGEILRCAARDLRAVKE 180
           I SILSH SM+ ALANHLSIKLCN +LPS TLFELF++V++ D GEILRC+ RDL+AVKE
Sbjct: 128 ITSILSHKSMERALANHLSIKLCNLSLPSSTLFELFTDVIQEDAGEILRCSIRDLQAVKE 187

Query: 181 RDPACISFVQCFLNYKGFLAVQSHRIANKLWSQGRKVLAIVIQNRVSEVFAVDIHPGAKI 240
           RDPA IS+VQCFLNYKGFLAVQSHRIA+KLW +GRKVLA++IQNRV+E FAVDIHPGA+I
Sbjct: 188 RDPAWISYVQCFLNYKGFLAVQSHRIAHKLWCRGRKVLALLIQNRVAEAFAVDIHPGARI 247

Query: 241 GCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTGKDSGDRHPKIGDGVLIGAGTCILG 300
           GCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTGK+SGDRHPKIGDGVLIGAGTCILG
Sbjct: 248 GCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTGKNSGDRHPKIGDGVLIGAGTCILG 307

Query: 301 NINIGDGAKIGAGSLVLKDVPPRTTAVGNPARLVGGKANPIKLDKTPSFTMDHTSHISEW 350
           NI IGDGAKIGAGS+VLKDVPPRTTAVGNPARL+GGKANPIKLDKTPSFTMDHTSHI++W
Sbjct: 308 NIKIGDGAKIGAGSVVLKDVPPRTTAVGNPARLIGGKANPIKLDKTPSFTMDHTSHINDW 367

BLAST of MC11g1026 vs. ExPASy TrEMBL
Match: A0A6J1J232 (Serine O-acetyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111480608 PE=3 SV=1)

HSP 1 Score: 523 bits (1348), Expect = 2.14e-184
Identity = 274/366 (74.86%), Postives = 306/366 (83.61%), Query Frame = 0

Query: 1   PLYRPLSRLHMSFVKPHSFNFSA-ISHLQELPRPSPMAA-----------CFDTTPLLPP 60
           PLYRPL RL M+   P  F+ S+ ISH  +L  PSPMAA           C  TTPL P 
Sbjct: 8   PLYRPLFRLPMALHNPSPFHLSSLISHSPDL-HPSPMAAACLHHNSPPLHCDPTTPL-PT 67

Query: 61  DPHFKIPQFCRSNFSDSVSCLPLPPK---FDSD-SDFDLWLKIRDEASADVEHEPILSNY 120
           DPH KI  F R  FSD +S  P+ P     +S  SD DLW KI++EA AD+EHEPIL++Y
Sbjct: 68  DPHLKILNFSRPTFSDHLSPPPISPNPQILNSPVSDSDLWFKIQEEARADMEHEPILASY 127

Query: 121 YIDSILSHASMDAALANHLSIKLCNSALPSPTLFELFSEVVRADGGEILRCAARDLRAVK 180
           YI SILSH SM+ ALANHLSIKLCN +LPS TLFELF++V++ D GEILRC+ RDL+AVK
Sbjct: 128 YITSILSHNSMERALANHLSIKLCNLSLPSSTLFELFTDVIQEDAGEILRCSIRDLQAVK 187

Query: 181 ERDPACISFVQCFLNYKGFLAVQSHRIANKLWSQGRKVLAIVIQNRVSEVFAVDIHPGAK 240
           ERDPA IS+VQCFLNYKGFLAVQSHRIA+KLW +GRKVLA++IQNRV+E FAVDIHPGA+
Sbjct: 188 ERDPAWISYVQCFLNYKGFLAVQSHRIAHKLWCRGRKVLALLIQNRVAEAFAVDIHPGAR 247

Query: 241 IGCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTGKDSGDRHPKIGDGVLIGAGTCIL 300
           IGCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTGK+SGDRHPKIGDGVLIGAGTCIL
Sbjct: 248 IGCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTGKNSGDRHPKIGDGVLIGAGTCIL 307

Query: 301 GNINIGDGAKIGAGSLVLKDVPPRTTAVGNPARLVGGKANPIKLDKTPSFTMDHTSHISE 350
           GNI IGDGAKIGAGS+VLKDVPPRTTAVGNPARL+GGKANPIKLDKTPSFTMDHTSHI++
Sbjct: 308 GNIKIGDGAKIGAGSVVLKDVPPRTTAVGNPARLIGGKANPIKLDKTPSFTMDHTSHIND 367

BLAST of MC11g1026 vs. ExPASy TrEMBL
Match: A0A6J1FE93 (Serine O-acetyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111443173 PE=3 SV=1)

HSP 1 Score: 513 bits (1321), Expect = 6.02e-181
Identity = 260/329 (79.03%), Postives = 282/329 (85.71%), Query Frame = 0

Query: 36  MAACF----DTTPLLPPDPHFKIPQFCRSNFSDSVSCLPL----------PPKFDSDSDF 95
           MAAC     D  P +  DPH K+ +FCR  FSD +S +P+          PP     SD 
Sbjct: 1   MAACLPLSGDPNPSISSDPHHKMVKFCRPTFSDHISSIPISPNPHKIQDSPPPPPPCSDS 60

Query: 96  DLWLKIRDEASADVEHEPILSNYYIDSILSHASMDAALANHLSIKLCNSALPSPTLFELF 155
           D+WLKIR+EA +DVE EPILSNYYI SILSH+SMD ALANHLSIKLC+S+LPS TLFELF
Sbjct: 61  DVWLKIREEARSDVEDEPILSNYYISSILSHSSMDRALANHLSIKLCSSSLPSTTLFELF 120

Query: 156 SEVVRADGGEILRCAARDLRAVKERDPACISFVQCFLNYKGFLAVQSHRIANKLWSQGRK 215
            +V+R DGGEILRC  RDLRAVKERDPACIS+VQCFLNYKGFLAVQSHRIA+KLWS GRK
Sbjct: 121 LDVLRDDGGEILRCGVRDLRAVKERDPACISYVQCFLNYKGFLAVQSHRIAHKLWSDGRK 180

Query: 216 VLAIVIQNRVSEVFAVDIHPGAKIGCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTG 275
           VLA++IQNRVSE FAVDIHPGA IGCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTG
Sbjct: 181 VLAMLIQNRVSETFAVDIHPGAVIGCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTG 240

Query: 276 KDSGDRHPKIGDGVLIGAGTCILGNINIGDGAKIGAGSLVLKDVPPRTTAVGNPARLVGG 335
           KDSGDRHPKIG+GVLIGAGTCILGNI IGD AKIGAGS+VLKDVPPRTTAVGNPARLVGG
Sbjct: 241 KDSGDRHPKIGNGVLIGAGTCILGNIKIGDRAKIGAGSVVLKDVPPRTTAVGNPARLVGG 300

Query: 336 KANPIKLDKTPSFTMDHTSHISEWSDYVI 350
           K+NPIKLDKTPSFTMDH SHI+EWSDYVI
Sbjct: 301 KSNPIKLDKTPSFTMDHISHINEWSDYVI 329

BLAST of MC11g1026 vs. ExPASy TrEMBL
Match: A0A0A0LDY4 (Serine O-acetyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G769110 PE=3 SV=1)

HSP 1 Score: 511 bits (1316), Expect = 5.17e-180
Identity = 267/350 (76.29%), Postives = 287/350 (82.00%), Query Frame = 0

Query: 11  MSFVKPHSFNFSAISHLQELPRPSPMAACFDTTPLLPPDPH--FKIPQFCRSNFSDSVSC 70
           MSF+   S  FS    L   P     AAC        PDP+   KI +FCR  FSD +S 
Sbjct: 1   MSFIPHFSSLFSPFPDLHLSPM---AAACL-------PDPNSSLKILKFCRPTFSDHLST 60

Query: 71  LPLPPK--------FDSDSDFDLWLKIRDEASADVEHEPILSNYYIDSILSHASMDAALA 130
           L + P             SD DLW  IR EA +DVEHEPILSNYYI SIL+HASMD ALA
Sbjct: 61  LSISPNPQNTHDSSLSPSSDSDLWFLIRQEACSDVEHEPILSNYYITSILTHASMDKALA 120

Query: 131 NHLSIKLCNSALPSPTLFELFSEVVRADGGEILRCAARDLRAVKERDPACISFVQCFLNY 190
           NHLSIKL NS+LPS TLF+LFS+V+  DGGEILRCA RDL+AVKERDPACIS+VQCFLNY
Sbjct: 121 NHLSIKLSNSSLPSSTLFQLFSDVLTDDGGEILRCAVRDLKAVKERDPACISYVQCFLNY 180

Query: 191 KGFLAVQSHRIANKLWSQGRKVLAIVIQNRVSEVFAVDIHPGAKIGCGILLDHATGVVVG 250
           KGFLAVQSHRIA+KLWS+GRKVLA++IQNRVSE FAVDIHPGAKIGCGILLDHATGVVVG
Sbjct: 181 KGFLAVQSHRIAHKLWSEGRKVLAMLIQNRVSEAFAVDIHPGAKIGCGILLDHATGVVVG 240

Query: 251 ETAVIGNDVSILHHVTLGGTGKDSGDRHPKIGDGVLIGAGTCILGNINIGDGAKIGAGSL 310
           ETAVIGNDVSILHHVTLGGTGKD+GDRHPKIGDGVLIGAGTCILGNI IGDGAKIGAGS+
Sbjct: 241 ETAVIGNDVSILHHVTLGGTGKDTGDRHPKIGDGVLIGAGTCILGNIKIGDGAKIGAGSV 300

Query: 311 VLKDVPPRTTAVGNPARLVGGKANPIKLDKTPSFTMDHTSHISEWSDYVI 350
           VLKDVPPRTTAVGNPARL+GGK NPIKLDKTPSFTMDHTSHISEWSDYVI
Sbjct: 301 VLKDVPPRTTAVGNPARLIGGKVNPIKLDKTPSFTMDHTSHISEWSDYVI 340

BLAST of MC11g1026 vs. TAIR 10
Match: AT3G13110.1 (serine acetyltransferase 2;2 )

HSP 1 Score: 403.7 bits (1036), Expect = 1.5e-112
Identity = 220/369 (59.62%), Postives = 264/369 (71.54%), Query Frame = 0

Query: 10  HMSFVKP----HSFNFSAISHLQELPRPSPMAACFDT----TPLLPPDP----------- 69
           H SF+ P      F    +S     P P PMAAC DT     P + P             
Sbjct: 26  HHSFLLPSFVSSKFKHHTLSPPPSPPPPPPMAACIDTCRTGKPQISPRDSSKHHDDESGF 85

Query: 70  ----HFKIPQFCRSNF----SDSVSCLPLPPKFDSDSDF-DLWLKIRDEASADVEHEPIL 129
               +F+ P   RS+F    + ++   PL    D D++  D+W KIR+EA +D+  EPI+
Sbjct: 86  RYMNYFRYPD--RSSFNGTQTKTLHTRPLLEDLDRDAEVDDVWAKIREEAKSDIAKEPIV 145

Query: 130 SNYYIDSILSHASMDAALANHLSIKLCNSALPSPTLFELFSEVVRADGGEILRCAARDLR 189
           S YY  SI+S  S++AALAN LS+KL N  LPS TLF+LFS V++ +  +I+     DL 
Sbjct: 146 SAYYHASIVSQRSLEAALANTLSVKLSNLNLPSNTLFDLFSGVLQGN-PDIVESVKLDLL 205

Query: 190 AVKERDPACISFVQCFLNYKGFLAVQSHRIANKLWSQGRKVLAIVIQNRVSEVFAVDIHP 249
           AVKERDPACIS+V CFL++KGFLA Q+HRIA++LW+Q RK+LA++IQNRVSE FAVD HP
Sbjct: 206 AVKERDPACISYVHCFLHFKGFLACQAHRIAHELWTQDRKILALLIQNRVSEAFAVDFHP 265

Query: 250 GAKIGCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTGKDSGDRHPKIGDGVLIGAGT 309
           GAKIG GILLDHAT +V+GETAV+GN+VSILH+VTLGGTGK  GDRHPKIGDGVLIGAGT
Sbjct: 266 GAKIGTGILLDHATAIVIGETAVVGNNVSILHNVTLGGTGKQCGDRHPKIGDGVLIGAGT 325

Query: 310 CILGNINIGDGAKIGAGSLVLKDVPPRTTAVGNPARLVGGKANPIKLDKTPSFTMDHTSH 351
           CILGNI IG+GAKIGAGS+VLKDVPPRTTAVGNPARL+GGK NP   DK P  TMD TSH
Sbjct: 326 CILGNITIGEGAKIGAGSVVLKDVPPRTTAVGNPARLLGGKDNPKTHDKIPGLTMDQTSH 385

BLAST of MC11g1026 vs. TAIR 10
Match: AT1G55920.1 (serine acetyltransferase 2;1 )

HSP 1 Score: 388.3 bits (996), Expect = 6.7e-108
Identity = 192/273 (70.33%), Postives = 229/273 (83.88%), Query Frame = 0

Query: 78  DSDFDLWLKIRDEASADVEHEPILSNYYIDSILSHASMDAALANHLSIKLCNSALPSPTL 137
           + D D+W+K+ +EA +DV+ EPILSNYY  SI SH S+++ALA+ LS+KL N  LPS TL
Sbjct: 43  EDDDDVWIKMLEEAKSDVKQEPILSNYYYASITSHRSLESALAHILSVKLSNLNLPSNTL 102

Query: 138 FELFSEVVRADGGEILRCAARDLRAVKERDPACISFVQCFLNYKGFLAVQSHRIANKLWS 197
           FELF  V+  +  EI+    +DL AVKERDPACIS+V CFL +KGFLA Q+HRIA+ LW 
Sbjct: 103 FELFISVLE-ESPEIIESTKQDLIAVKERDPACISYVHCFLGFKGFLACQAHRIAHTLWK 162

Query: 198 QGRKVLAIVIQNRVSEVFAVDIHPGAKIGCGILLDHATGVVVGETAVIGNDVSILHHVTL 257
           Q RK++A++IQNRVSE FAVDIHPGAKIG GILLDHATGVV+GETAV+G++VSILH VTL
Sbjct: 163 QNRKIVALLIQNRVSESFAVDIHPGAKIGKGILLDHATGVVIGETAVVGDNVSILHGVTL 222

Query: 258 GGTGKDSGDRHPKIGDGVLIGAGTCILGNINIGDGAKIGAGSLVLKDVPPRTTAVGNPAR 317
           GGTGK SGDRHPKIGDGVLIGAG+CILGNI IG+GAKIG+GS+V+KDVP RTTAVGNPAR
Sbjct: 223 GGTGKQSGDRHPKIGDGVLIGAGSCILGNITIGEGAKIGSGSVVVKDVPARTTAVGNPAR 282

Query: 318 LVGGKANPIKLDKTPSFTMDHTSHISEWSDYVI 351
           L+GGK NP K DK P  TMD TS+++EWSDYVI
Sbjct: 283 LIGGKENPRKHDKIPCLTMDQTSYLTEWSDYVI 314

BLAST of MC11g1026 vs. TAIR 10
Match: AT5G56760.1 (serine acetyltransferase 1;1 )

HSP 1 Score: 339.7 bits (870), Expect = 2.7e-93
Identity = 171/269 (63.57%), Postives = 207/269 (76.95%), Query Frame = 0

Query: 83  LWLKIRDEASADVEHEPILSNYYIDSILSHASMDAALANHLSIKLCNSALPSPTLFELFS 142
           LW +I+ EA  D E EP L++Y   +ILSH+S++ +++ HL  KLC+S L S  L++LF 
Sbjct: 45  LWTQIKAEARRDAEAEPALASYLYSTILSHSSLERSISFHLGNKLCSSTLLSTLLYDLFL 104

Query: 143 EVVRADGGEILRCAARDLRAVKERDPACISFVQCFLNYKGFLAVQSHRIANKLWSQGRKV 202
               +D   +      DLRA + RDPACISF  C LNYKGFLA+Q+HR+++KLW+Q RK 
Sbjct: 105 NTFSSD-PSLRNATVADLRAARVRDPACISFSHCLLNYKGFLAIQAHRVSHKLWTQSRKP 164

Query: 203 LAIVIQNRVSEVFAVDIHPGAKIGCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTGK 262
           LA+ + +R+S+VFAVDIHP AKIG GILLDHATGVVVGETAVIGN+VSILHHVTLGGTGK
Sbjct: 165 LALALHSRISDVFAVDIHPAAKIGKGILLDHATGVVVGETAVIGNNVSILHHVTLGGTGK 224

Query: 263 DSGDRHPKIGDGVLIGAGTCILGNINIGDGAKIGAGSLVLKDVPPRTTAVGNPARLVGGK 322
             GDRHPKIGDG LIGAG  ILGN+ IG GAK+GAGS+VL DVP R TAVGNPARLVGGK
Sbjct: 225 ACGDRHPKIGDGCLIGAGATILGNVKIGAGAKVGAGSVVLIDVPCRGTAVGNPARLVGGK 284

Query: 323 ANP-IKLDKTPSFTMDHTSHISEWSDYVI 351
             P I  ++ P  +MDHTS ISEWSDY+I
Sbjct: 285 EKPTIHDEECPGESMDHTSFISEWSDYII 312

BLAST of MC11g1026 vs. TAIR 10
Match: AT4G35640.1 (serine acetyltransferase 3;2 )

HSP 1 Score: 271.6 bits (693), Expect = 9.1e-73
Identity = 142/269 (52.79%), Postives = 189/269 (70.26%), Query Frame = 0

Query: 70  PLPPKFDSDSDFDLWLKIRDEASADVEHEPILSNYYIDSILSHASMDAALANHLSIKLCN 129
           P+   F + S   +W  IR+EA  + E EP+LS++   SILSH  ++ AL+  L+ +L N
Sbjct: 60  PVLLDFTNSSYDPIWDSIREEAKLEAEEEPVLSSFLYASILSHDCLEQALSFVLANRLQN 119

Query: 130 SALPSPTLFELFSEVVRADGGEILRCAARDLRAVKERDPACISFVQCFLNYKGFLAVQSH 189
             L +  L ++F  V+  D G I      D++A K+RDPAC+S+    L+ KG+LA+Q++
Sbjct: 120 PTLLATQLMDIFCNVMVHDRG-IQSSIRLDVQAFKDRDPACLSYSSAILHLKGYLALQAY 179

Query: 190 RIANKLWSQGRKVLAIVIQNRVSEVFAVDIHPGAKIGCGILLDHATGVVVGETAVIGNDV 249
           R+A+KLW QGRK+LA+ +Q+RVSEVF +DIHP A+IG GILLDH TGVV+GETAVIG+ V
Sbjct: 180 RVAHKLWKQGRKLLALALQSRVSEVFGIDIHPAARIGKGILLDHGTGVVIGETAVIGDRV 239

Query: 250 SILHHVTLGGTGKDSGDRHPKIGDGVLIGAGTCILGNINIGDGAKIGAGSLVLKDVPPRT 309
           SILH VTLGGTGK++GDRHP IGDG L+GA   ILGNI IG GA + AGSLVLKDVP  +
Sbjct: 240 SILHGVTLGGTGKETGDRHPNIGDGALLGACVTILGNIKIGAGAMVAAGSLVLKDVPSHS 299

Query: 310 TAVGNPARLVGGKANPIKLDKTPSFTMDH 339
              GNPA+L+G        ++ PS TM+H
Sbjct: 300 MVAGNPAKLIG-----FVDEQDPSMTMEH 322

BLAST of MC11g1026 vs. TAIR 10
Match: AT2G17640.1 (Trimeric LpxA-like enzymes superfamily protein )

HSP 1 Score: 263.8 bits (673), Expect = 1.9e-70
Identity = 135/256 (52.73%), Postives = 182/256 (71.09%), Query Frame = 0

Query: 83  LWLKIRDEASADVEHEPILSNYYIDSILSHASMDAALANHLSIKLCNSALPSPTLFELFS 142
           +W  IR+EA  + E EPILS++    IL+H  ++ AL   L+ +L N  L +  L ++F 
Sbjct: 36  IWDAIREEAKLEAEKEPILSSFLYAGILAHDCLEQALGFVLANRLQNPTLLATQLLDIFY 95

Query: 143 EVVRADGGEILRCAARDLRAVKERDPACISFVQCFLNYKGFLAVQSHRIANKLWSQGRKV 202
            V+  D G I      DL+A K+RDPAC+S+    L+ KG+ A+Q++R+A+KLW++GRK+
Sbjct: 96  GVMMHDKG-IQSSIRHDLQAFKDRDPACLSYSSAILHLKGYHALQAYRVAHKLWNEGRKL 155

Query: 203 LAIVIQNRVSEVFAVDIHPGAKIGCGILLDHATGVVVGETAVIGNDVSILHHVTLGGTGK 262
           LA+ +Q+R+SEVF +DIHP A+IG GILLDH TGVV+GETAVIGN VSILH VTLGGTGK
Sbjct: 156 LALALQSRISEVFGIDIHPAARIGEGILLDHGTGVVIGETAVIGNGVSILHGVTLGGTGK 215

Query: 263 DSGDRHPKIGDGVLIGAGTCILGNINIGDGAKIGAGSLVLKDVPPRTTAVGNPARLVGGK 322
           ++GDRHPKIG+G L+GA   ILGNI+IG GA + AGSLVLKDVP  +   GNPA+L+   
Sbjct: 216 ETGDRHPKIGEGALLGACVTILGNISIGAGAMVAAGSLVLKDVPSHSVVAGNPAKLI--- 275

Query: 323 ANPIKLDKTPSFTMDH 339
              +  ++ PS  M H
Sbjct: 276 --RVMEEQDPSLAMKH 285

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q392182.2e-11159.62Serine acetyltransferase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SAT... [more]
Q425889.4e-10770.33Serine acetyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SAT... [more]
Q425383.8e-9263.57Serine acetyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SAT5 PE=1 SV=1[more]
Q8W0E42.3e-8961.43Probable serine acetyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q0DGG82.3e-8456.94Probable serine acetyltransferase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Match NameE-valueIdentityDescription
XP_038900115.12.00e-18877.44serine acetyltransferase 1, chloroplastic-like [Benincasa hispida][more]
XP_004150470.27.28e-18876.94serine acetyltransferase 1, chloroplastic [Cucumis sativus] >KAE8645986.1 hypoth... [more]
KAG6607765.15.68e-18775.07Serine acetyltransferase 1, chloroplastic, partial [Cucurbita argyrosperma subsp... [more]
XP_008462963.13.84e-18677.09PREDICTED: serine acetyltransferase 1, chloroplastic [Cucumis melo][more]
XP_022940668.11.33e-18575.07serine acetyltransferase 1, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A1S3CI271.86e-18677.09Serine O-acetyltransferase OS=Cucumis melo OX=3656 GN=LOC103501225 PE=3 SV=1[more]
A0A6J1FPY36.44e-18675.07Serine O-acetyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446191 PE=3 SV... [more]
A0A6J1J2322.14e-18474.86Serine O-acetyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111480608 PE=3 SV=1[more]
A0A6J1FE936.02e-18179.03Serine O-acetyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111443173 PE=3 SV... [more]
A0A0A0LDY45.17e-18076.29Serine O-acetyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G769110 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G13110.11.5e-11259.62serine acetyltransferase 2;2 [more]
AT1G55920.16.7e-10870.33serine acetyltransferase 2;1 [more]
AT5G56760.12.7e-9363.57serine acetyltransferase 1;1 [more]
AT4G35640.19.1e-7352.79serine acetyltransferase 3;2 [more]
AT2G17640.11.9e-7052.73Trimeric LpxA-like enzymes superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010493Serine acetyltransferase, N-terminalSMARTSM00971SATase_N_2_acoord: 83..188
e-value: 2.5E-44
score: 163.3
IPR010493Serine acetyltransferase, N-terminalPFAMPF06426SATase_Ncoord: 83..188
e-value: 6.5E-32
score: 110.0
IPR005881Serine O-acetyltransferaseTIGRFAMTIGR01172TIGR01172coord: 158..317
e-value: 7.5E-68
score: 225.6
IPR042122Serine acetyltransferase, N-terminal domain superfamilyGENE3D1.10.3130.10serine acetyltransferase, domain 1coord: 74..217
e-value: 1.3E-46
score: 160.4
NoneNo IPR availableGENE3D2.160.10.10Hexapeptide repeat proteinscoord: 218..337
e-value: 4.9E-39
score: 134.8
NoneNo IPR availablePANTHERPTHR42811:SF19SERINE O-ACETYLTRANSFERASE, SERINE ACETYLTRANSFERASE-RELATEDcoord: 56..350
NoneNo IPR availablePANTHERPTHR42811SERINE ACETYLTRANSFERASEcoord: 56..350
NoneNo IPR availableCDDcd03354LbH_SATcoord: 215..315
e-value: 2.0417E-53
score: 169.543
IPR001451Hexapeptide repeatPFAMPF00132Hexapepcoord: 268..301
e-value: 2.5E-8
score: 33.3
IPR018357Hexapeptide transferase, conserved sitePROSITEPS00101HEXAPEP_TRANSFERASEScoord: 277..305
IPR011004Trimeric LpxA-like superfamilySUPERFAMILY51161Trimeric LpxA-like enzymescoord: 83..319

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC11g1026.1MC11g1026.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006535 cysteine biosynthetic process from serine
cellular_component GO:0005829 cytosol
cellular_component GO:0005737 cytoplasm
molecular_function GO:0009001 serine O-acetyltransferase activity
molecular_function GO:0016740 transferase activity