MC11g1011 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g1011
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	MBOAT (membrane bound O-acyl transferase) family protein
Location	MC11: 8650613 .. 8651621 (-)
RNA-Seq Expression	MC11g1011
Synteny	MC11g1011

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGGCCGAACTCCATGCCCTAATCAAGGTATCCCTCTGTGTTTTCGCATCTATTCTCTACTCCTATTTCGTAGCTTCAAAGCTTCCCAAAGGCAAATTCAGGCTCCTCTCTCTCCTCCCAATTTTCCCTCTCTTCGCCCTCCTCCCTCCTCCTCTCCGGAATCGTCGCCTTCTTCATCACCTGGCTCGCCTCCTTCAAGCTCCTCTTCTTCTCCTTCGATTCCGGCCCTCTCGCTTCCGATCCGCCATTGCCCTTCCCTCTCTTCGCCTCAATCGCTTGCTTCCCCATCCGAATCAAGCAAAATCCTCCCAAAACCCTACATCCGCCTCCACATCTCCCCGATTCCCCAAAATTGCCTCTCAATCTGCCGGCGAAAGCGTTGCTATTCGCCGCCCTGGTCGCCGCCGACGATTTCGCCCAATCTCTCCCCCAAAACGCGAAACTCTGCTTCTATTGCGCGATTCTGTATCCGTTTATCGACGTTGTTTTGGGCCTCAGTAATGCGATTGTTCGATCGGCGTTCGGGATCGAGCTCGAACAGCCCTCCAACGAGCCCTATTTGGCGACGTCGCTACAGGATTTTTGGGGGCGGCGCTGGAATCTGTTGGTGTCGAATACTTTGAGGTACACGGTTTATAGGCCGGTCCGCGCAGCATTGGGGCGCCGGCGGGGGGCGGCGCCGGCGGCGGTGTTCGCGACGTTTGTGGTGTCGGGGCTGATGCACGAGCTACTGTTTTACTACGTCACCAGAGCGGTTCCGGCGTGGGAGGTTACGGCGTTTTTCGTCCTTCATGGGGTGTGTGTGGTGGTGGAATTTAGAGTGAAGAGGGCGGTGGGGGAAAAGTGGCGGTTGCATTGGGCGGTTTCCGGGCCGTTGACGGTGGCGTTCGTGGTGGTCACCGGCGCGTGGCTGTTCTTTCCGCCGTTGTTGAGGACTGGCGCGGTAGTCGGAGTTCTTCGGGAGTGTAAGTCTGCGGCGGAGTTCGTTAAAGCTCAGGTCTCATCCGTC

mRNA sequence

GCGGCCGAACTCCATGCCCTAATCAAGGTATCCCTCTGTGTTTTCGCATCTATTCTCTACTCCTATTTCGTAGCTTCAAAGCTTCCCAAAGGCAAATTCAGGCTCCTCTCTCTCCTCCCAATTTTCCCTCTCTTCGCCCTCCTCCCTCTCCTCTCCGGAATCGTCGCCTTCTTCATCACCTGGCTCGCCTCCTTCAAGCTCCTCTTCTTCTCCTTCGATTCCGGCCCTCTCGCTTCCGATCCGCCATTGCCCTTCCCTCTCTTCGCCTCAATCGCTTGCTTCCCCATCCGAATCAAGCAAAATCCTCCCAAAACCCTACATCCGCCTCCACATCTCCCCGATTCCCCAAAATTGCCTCTCAATCTGCCGGCGAAAGCGTTGCTATTCGCCGCCCTGGTCGCCGCCGACGATTTCGCCCAATCTCTCCCCCAAAACGCGAAACTCTGCTTCTATTGCGCGATTCTGTATCCGTTTATCGACGTTGTTTTGGGCCTCAGTAATGCGATTGTTCGATCGGCGTTCGGGATCGAGCTCGAACAGCCCTCCAACGAGCCCTATTTGGCGACGTCGCTACAGGATTTTTGGGGGCGGCGCTGGAATCTGTTGGTGTCGAATACTTTGAGGTACACGGTTTATAGGCCGGTCCGCGCAGCATTGGGGCGCCGGCGGGGGGCGGCGCCGGCGGCGGTGTTCGCGACGTTTGTGGTGTCGGGGCTGATGCACGAGCTACTGTTTTACTACGTCACCAGAGCGGTTCCGGCGTGGGAGGTTACGGCGTTTTTCGTCCTTCATGGGGTGTGTGTGGTGGTGGAATTTAGAGTGAAGAGGGCGGTGGGGGAAAAGTGGCGGTTGCATTGGGCGGTTTCCGGGCCGTTGACGGTGGCGTTCGTGGTGGTCACCGGCGCGTGGCTGTTCTTTCCGCCGTTGTTGAGGACTGGCGCGGTAGTCGGAGTTCTTCGGGAGTGTAAGTCTGCGGCGGAGTTCGTTAAAGCTCAGGTCTCATCCGTC

Coding sequence (CDS)

GCGGCCGAACTCCATGCCCTAATCAAGGTATCCCTCTGTGTTTTCGCATCTATTCTCTACTCCTATTTCGTAGCTTCAAAGCTTCCCAAAGGCAAATTCAGGCTCCTCTCTCTCCTCCCAATTTTCCCTCTCTTCGCCCTCCTCCCTCTCCTCTCCGGAATCGTCGCCTTCTTCATCACCTGGCTCGCCTCCTTCAAGCTCCTCTTCTTCTCCTTCGATTCCGGCCCTCTCGCTTCCGATCCGCCATTGCCCTTCCCTCTCTTCGCCTCAATCGCTTGCTTCCCCATCCGAATCAAGCAAAATCCTCCCAAAACCCTACATCCGCCTCCACATCTCCCCGATTCCCCAAAATTGCCTCTCAATCTGCCGGCGAAAGCGTTGCTATTCGCCGCCCTGGTCGCCGCCGACGATTTCGCCCAATCTCTCCCCCAAAACGCGAAACTCTGCTTCTATTGCGCGATTCTGTATCCGTTTATCGACGTTGTTTTGGGCCTCAGTAATGCGATTGTTCGATCGGCGTTCGGGATCGAGCTCGAACAGCCCTCCAACGAGCCCTATTTGGCGACGTCGCTACAGGATTTTTGGGGGCGGCGCTGGAATCTGTTGGTGTCGAATACTTTGAGGTACACGGTTTATAGGCCGGTCCGCGCAGCATTGGGGCGCCGGCGGGGGGCGGCGCCGGCGGCGGTGTTCGCGACGTTTGTGGTGTCGGGGCTGATGCACGAGCTACTGTTTTACTACGTCACCAGAGCGGTTCCGGCGTGGGAGGTTACGGCGTTTTTCGTCCTTCATGGGGTGTGTGTGGTGGTGGAATTTAGAGTGAAGAGGGCGGTGGGGGAAAAGTGGCGGTTGCATTGGGCGGTTTCCGGGCCGTTGACGGTGGCGTTCGTGGTGGTCACCGGCGCGTGGCTGTTCTTTCCGCCGTTGTTGAGGACTGGCGCGGTAGTCGGAGTTCTTCGGGAGTGTAAGTCTGCGGCGGAGTTCGTTAAAGCTCAGGTCTCATCCGTC

Protein sequence

AAELHALIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLPLLSGIVAFFITWLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQNPPKTLHPPPHLPDSPKLPLNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIVRSAFGIELEQPSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRPVRAALGRRRGAAPAAVFATFVVSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKWRLHWAVSGPLTVAFVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEFVKAQVSSV

Homology

[Close]

BLAST of MC11g1011 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 8.5e-65
Identity = 153/341 (44.87%), Postives = 195/341 (57.18%), Query Frame = 0


Query: 3   ELHALIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLPL------LSGIVA 62
           EL  LIKV +    SI Y Y++  ++  G  R LS+ P+  LF +LPL      LS I A
Sbjct: 4   ELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSLITA 63

Query: 63  FFITWLASFKLLFFSFDSGPLASDPPLP--FPLFASIACFPIRIKQNPPKTLHPPPHLPD 122
           FF+TWLA+FKL+ FSFD GPL    P+P  FP F    CFPI+++QNP    H P     
Sbjct: 64  FFLTWLANFKLILFSFDKGPLI---PIPTNFPRFFCFTCFPIKVQQNPKSQNHLP----- 123

Query: 123 SPKLPLNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIVRSAF 182
                L    K  +FA L+    + Q+L     L  Y   LY  I+++L     +V  + 
Sbjct: 124 ----KLVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISL 183

Query: 183 GIELEQPSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRPVRAALGRRRGAAPA---AVF 242
           G +LE  SN+PYLATSLQDFWGRRWNL+V   LR  VY P+R    R+  +  A    + 
Sbjct: 184 GCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGIL 243

Query: 243 ATFVVSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKR--AVGEKWRLHWAVS 302
           A F+VSGL+HELLF+Y+ R +P  EVT FFVLHGVC  VE  VK+   V ++WRL   VS
Sbjct: 244 AAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSPGVS 303

Query: 303 GPLTVAFVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEFVK 331
             LTV FV VTG WLF P L R+G +     E     EF+K
Sbjct: 304 RVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIK 332

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BLAST of MC11g1011 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.2e-60
Identity = 148/340 (43.53%), Postives = 189/340 (55.59%), Query Frame = 0


Query: 7   LIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLPLLSGIVAF------FIT 66
           LI+V +    S+ Y Y+++SKL KG  RLLS+LP+  LF +LPL    V F      F++
Sbjct: 8   LIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCAISVLFLS 67

Query: 67  WLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQNPPKTLHPPPHLPDSPKLPL 126
           WLA+FKLL F+FD GPL   PP     F   AC PI+I+Q+P     P  H    PK   
Sbjct: 68  WLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQDPSPNAIPNLHPKPMPKWV- 127

Query: 127 NLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIVRSAFGIELEQ 186
            L  K L+   L+   ++   LP+   L  YC  +Y  +++VL    A+V +  G  +E 
Sbjct: 128 -LAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLLGCNIEP 187

Query: 187 PSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRPVRAALGR---RRGAAPAAVFATFVVS 246
             NEPYLATSLQDFW RRWNL+VS  LR TV+ PV+    R     GA  A V A+F VS
Sbjct: 188 VFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVMASFFVS 247

Query: 247 GLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKWRL--HWAVSGPLTVA 306
           GLMHELL++Y+ R  P WEVT FFVLHG     E  VKR    +W    H AVSG + + 
Sbjct: 248 GLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRT---QWLRPPHRAVSGLVVLT 307

Query: 307 FVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEFVKAQVSS 336
           FV VTG WLF   +LR       + EC    +  K   SS
Sbjct: 308 FVSVTGVWLFLAQVLRNNVHEKAIGECLLVLDLAKLFTSS 341

[Close]

BLAST of MC11g1011 vs. ExPASy Swiss-Prot
Match: Q9FJ74 (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=AT3 PE=3 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.2e-60
Identity = 152/342 (44.44%), Postives = 202/342 (59.06%), Query Frame = 0


Query: 3   ELHALIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLPL------LSGIVA 62
           EL   IK+ +    SI Y Y++++ +  G FRLLS+LP+  LF + PL       SG +A
Sbjct: 4   ELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSGCMA 63

Query: 63  FFITWLASFKLLFFSFDSGPLASDPPLPFPL--FASIACFPIRIKQNPPKTLHPPPHLPD 122
           FF++WLA+FKL+ FSFD GPL+   PLP  L  F  I CFPI+ +QNP            
Sbjct: 64  FFLSWLANFKLILFSFDQGPLS---PLPRTLSRFICITCFPIKPQQNPN---------IQ 123

Query: 123 SPKLPLNLPA-KALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIVRSA 182
           + K+P+ L A K ++F  L+   ++ Q L     L F    ++  +++V  L  A+V   
Sbjct: 124 NYKIPIWLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFIT 183

Query: 183 FGIELEQPSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRPVRAALGRRRGAAPA---AV 242
            G +LE  SNEPYLATSLQDFWGRRWNL+V   LR  VY P R    R+  +  A    V
Sbjct: 184 LGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGV 243

Query: 243 FATFVVSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRA--VGEKWRLHWAV 302
           FA F+VSG +HE+LF+Y+TR VP  EVT FF+LHGVC V E  VK++  V   WR+   V
Sbjct: 244 FAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTV 303

Query: 303 SGPLTVAFVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEFVK 331
           S  LTV FVVVT  W FF PL+R+G +  +  E     +FVK
Sbjct: 304 SRLLTVGFVVVTSGWFFF-PLIRSGIIERLASEALMCIDFVK 332

[Close]

BLAST of MC11g1011 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.7e-60
Identity = 144/340 (42.35%), Postives = 193/340 (56.76%), Query Frame = 0


Query: 3   ELHALIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLPL------LSGIVA 62
           EL +LIKV +    SI Y Y+ ++++  G FRLLS+LP+  LF +LPL       SG  A
Sbjct: 4   ELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSGSTA 63

Query: 63  FFITWLASFKLLFFSFDSGPLASDPPLPFPL--FASIACFPIRIKQNPPKTLHPPPHLPD 122
           FF++WLA+FKL+ FSFD GPL    P+P  L  F    CFPI+++QNP          P 
Sbjct: 64  FFLSWLANFKLILFSFDQGPLF---PVPSNLSRFVCFTCFPIKLQQNPK---------PQ 123

Query: 123 SPKLPLNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIVRSAF 182
           +         K   F  L+   ++ Q +     L  Y   +Y   +++L     ++  + 
Sbjct: 124 NQMPKWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISL 183

Query: 183 GIELEQPSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRPVRAALGRRRGAAPA---AVF 242
             +LE   NEPYL+TSLQDFWGRRWNL+V   LR  VY PVR   GR+  +  A    VF
Sbjct: 184 WCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVF 243

Query: 243 ATFVVSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRA-VGEKWRLHWAVSG 302
           A+F+VSG++HEL+F+Y TR  P  EVT FFVLHGVC   E   KR  +  +W++   VS 
Sbjct: 244 ASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSR 303

Query: 303 PLTVAFVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEFVK 331
            LTV FVV+TG WLFFP L R+G +  +  E      FVK
Sbjct: 304 LLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVK 331

[Close]

BLAST of MC11g1011 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.2e-58
Identity = 136/333 (40.84%), Postives = 194/333 (58.26%), Query Frame = 0


Query: 6   ALIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLPL------LSGIVAFFI 65
           + IK    V  S+ Y++F+A  +PKG  RL+   P+F +F ++P       L GI AFFI
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 66  TWLASFKLLFFSFDSGPLASD-PPLPFPLFASIACFPIRIKQNPPKTLHPPPHLPDSPKL 125
            WLA+FKLL F+   GPL+S+  PL  P+F +++C PI+I Q  PK      H   S + 
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKI-QLSPKPTKTHSH-EGSTEG 122

Query: 126 PLNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIVRSAFGIEL 185
           PL    KA+    ++ A +++  LP+   L  Y   +Y  ++++L  + A VR+   +EL
Sbjct: 123 PLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLEL 182

Query: 186 EQPSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRP---VRAALGRRRGAAPAAVFATFV 245
           E   N+PYLATSLQDFWGRRWNL+V+  LR TVY P   + + LG       AA F TFV
Sbjct: 183 EPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFSVLGPNYSQILAA-FGTFV 242

Query: 246 VSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKWRLHWAVSGPLTVA 305
           VSG+MHEL+F+Y+ R  P W++  FF+++G C  VE  +K+ +  +WR   A+S  LT+ 
Sbjct: 243 VSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLT 302

Query: 306 FVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEF 329
           FV+VT  WLF P   R   V   L E  +   F
Sbjct: 303 FVMVTALWLFLPEFNRCNIVEKALDEYAAIGAF 332

[Close]

BLAST of MC11g1011 vs. NCBI nr
Match: XP_022139430.1 (LOW QUALITY PROTEIN: probable long-chain-alcohol O-fatty-acyltransferase 5 [Momordica charantia])

HSP 1 Score: 650 bits (1677), Expect = 7.05e-235
Identity = 334/337 (99.11%), Postives = 335/337 (99.41%), Query Frame = 0


Query: 1   AAELHALIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLP-LLSGIVAFFI 60
           AAELHALIKVSLCVFAS+LYSYFVASKLPKGKFRLLSLLPIFPLFALLP LLSGIVAFFI
Sbjct: 2   AAELHALIKVSLCVFASLLYSYFVASKLPKGKFRLLSLLPIFPLFALLPXLLSGIVAFFI 61

Query: 61  TWLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQNPPKTLHPPPHLPDSPKLP 120
           TWLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQNPPKTLHPPPHLPDSPKLP
Sbjct: 62  TWLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQNPPKTLHPPPHLPDSPKLP 121

Query: 121 LNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIVRSAFGIELE 180
           LNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIVRSAFGIELE
Sbjct: 122 LNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIVRSAFGIELE 181

Query: 181 QPSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRPVRAALGRRRGAAPAAVFATFVVSGL 240
           QPSNEPYLATSLQDFWGRRWNLLVSNTLRY VYRPVRAALGRRRGAAPAAVFATFVVSGL
Sbjct: 182 QPSNEPYLATSLQDFWGRRWNLLVSNTLRYXVYRPVRAALGRRRGAAPAAVFATFVVSGL 241

Query: 241 MHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKWRLHWAVSGPLTVAFVVV 300
           MHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKWRLHWAVSGPLTVAFVVV
Sbjct: 242 MHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKWRLHWAVSGPLTVAFVVV 301

Query: 301 TGAWLFFPPLLRTGAVVGVLRECKSAAEFVKAQVSSV 336
           TGAWLFFPPLLRTGAVVGVLRECKSAAEFVKAQVSSV
Sbjct: 302 TGAWLFFPPLLRTGAVVGVLRECKSAAEFVKAQVSSV 338

[Close]

BLAST of MC11g1011 vs. NCBI nr
Match: XP_023525214.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 474 bits (1220), Expect = 3.34e-165
Identity = 253/348 (72.70%), Postives = 279/348 (80.17%), Query Frame = 0


Query: 1   AAELHALIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLPL------LSGI 60
           AA+L+ L+K+S  V AS+LYSYF+++KLPKG  RLLSLLPI  L+ LLPL      L+GI
Sbjct: 2   AADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGI 61

Query: 61  VAFFITWLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQNPP--------KTL 120
           VAFFITWLASFKLL F FDSGPL +DPPL FPLF SIACFPIRIKQN P        K  
Sbjct: 62  VAFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKPT 121

Query: 121 HPPPHLPDSPKLPLNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLS 180
            PP    DSPKLPLNLPAK  LFA LVA DDFAQ+L  NA LCFYC  LY FIDVVLGLS
Sbjct: 122 DPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGLS 181

Query: 181 NAIVRSAFGIELEQPSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRPVRAA----LGRR 240
           N  VR  FGIELE PSNEPYLATSL+DFWGRRWNLLVSNTLR++V+ PVRAA    LGRR
Sbjct: 182 NTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRR 241

Query: 241 RGAAPAAVFATFVVSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKW 300
           R AA  AVFATFVVSGLMHELLFYYVT AVP WEVT FFVLHGVCVV+EF VK+A+GE W
Sbjct: 242 R-AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKALGENW 301

Query: 301 RLHWAVSGPLTVAFVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEFVK 330
           RLHWAV+GPLT+AFVV+T AWLFFPPLL+TGAV  V+ ECK+AA +VK
Sbjct: 302 RLHWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAAAGYVK 348

[Close]

BLAST of MC11g1011 vs. NCBI nr
Match: XP_022981530.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita maxima])

HSP 1 Score: 467 bits (1201), Expect = 2.59e-162
Identity = 248/348 (71.26%), Postives = 275/348 (79.02%), Query Frame = 0


Query: 1   AAELHALIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLPL------LSGI 60
           AA+L+ L+K+S  V AS+LYSYF++SKLPKG  RLLSLLPI  L+ LLPL      L+GI
Sbjct: 2   AADLYTLLKISAAVLASLLYSYFISSKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGI 61

Query: 61  VAFFITWLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQN--------PPKTL 120
           VAFFITWLASFKLL F FDSGPL SDPPL FPLF SIAC+PIRIKQN        P K  
Sbjct: 62  VAFFITWLASFKLLLFCFDSGPLFSDPPLSFPLFVSIACYPIRIKQNQYDPDPQNPQKPT 121

Query: 121 HPPPHLPDSPKLPLNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLS 180
            PP    DSPKLPLNLPAK  LFA LVA DDFAQ+L  N  LCFYC  LY FIDVVLG+ 
Sbjct: 122 DPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNTMLCFYCVSLYLFIDVVLGIC 181

Query: 181 NAIVRSAFGIELEQPSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRPVRAAL----GRR 240
           N  VR  FGIELE PSNEPYLATSL+DFWGRRWNLLVSNTLR++V+ PVRAA+    GRR
Sbjct: 182 NTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVVGRR 241

Query: 241 RGAAPAAVFATFVVSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKW 300
           R AA  AVFATFVVSGLMHEL+FYYVT A+P WEVT FFVLHGVCVVVEF VK A+GE W
Sbjct: 242 R-AAVVAVFATFVVSGLMHELVFYYVTGAIPTWEVTGFFVLHGVCVVVEFWVKMALGENW 301

Query: 301 RLHWAVSGPLTVAFVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEFVK 330
           RLHWAV+GPLT+AFVV+T AWLFFPPLL+TG V  V+ ECK+AA +VK
Sbjct: 302 RLHWAVAGPLTLAFVVITAAWLFFPPLLKTGVVAAVIGECKAAAGYVK 348

[Close]

BLAST of MC11g1011 vs. NCBI nr
Match: XP_022940347.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X1 [Cucurbita moschata])

HSP 1 Score: 463 bits (1191), Expect = 8.29e-161
Identity = 248/347 (71.47%), Postives = 273/347 (78.67%), Query Frame = 0


Query: 2   AELHALIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLPL------LSGIV 61
           A+L+ L+K+S  V AS+LYSYF++SKLPKG  RLLSLLPI  L+ LLPL      L+GIV
Sbjct: 2   ADLYTLLKISAAVLASLLYSYFISSKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIV 61

Query: 62  AFFITWLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQNPPKTLHPPPHLP-- 121
           AFFITWLASFKLL F FDSGPL SDPPL FPLF SI+CFPIRIKQN P      P  P  
Sbjct: 62  AFFITWLASFKLLLFCFDSGPLFSDPPLSFPLFVSISCFPIRIKQNQPDPDPQNPQKPTD 121

Query: 122 ------DSPKLPLNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSN 181
                 DSPKL LNLPAK  LFA LVA DDFAQ+L  NA LCFYC  LY FIDV+LGL N
Sbjct: 122 PLLKTIDSPKLRLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYFFIDVILGLCN 181

Query: 182 AIVRSAFGIELEQPSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRPVRAA----LGRRR 241
             VR  FGIELE PSNEPYLATSL+DFWGRRWNLLVSNTLR++V+ PVRAA    LGRRR
Sbjct: 182 TFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR 241

Query: 242 GAAPAAVFATFVVSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKWR 301
            AA  AVFATFVVSGLMHELLFYYVT AVP WEVT FFVLHGVCVVVEF VK  +GE WR
Sbjct: 242 -AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVVEFWVKMVLGENWR 301

Query: 302 LHWAVSGPLTVAFVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEFVK 330
           LHWAV+GPLT+AFVV+T AWLFFPPLL+TGAV  ++ ECK+AA +VK
Sbjct: 302 LHWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAIIGECKAAAGYVK 347

[Close]

BLAST of MC11g1011 vs. NCBI nr
Match: KAG6607778.1 (putative long-chain-alcohol O-fatty-acyltransferase 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 459 bits (1180), Expect = 1.25e-158
Identity = 247/345 (71.59%), Postives = 269/345 (77.97%), Query Frame = 0


Query: 7   LIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLPL------LSGIVAFFIT 66
           L+K+S  V AS+LYSYF++SKLPKG  RLLSLLPI  L+ LLPL      L+GIVAFFIT
Sbjct: 37  LLKISAAVLASLLYSYFISSKLPKGLPRLLSLLPILSLYTLLPLPLSSPLLAGIVAFFIT 96

Query: 67  WLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQNPPKTLHPPPHLP------- 126
           WLASFKLL F FDSGPL SDPPL FPLF SIACFPIRIKQN P      P  P       
Sbjct: 97  WLASFKLLLFFFDSGPLFSDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQNPQKPTDSL 156

Query: 127 ----DSPKLPLNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAI 186
               DSPKLPLNLPAK  LFA LVA DDFAQ+L  NA LCFYC  LY FIDV+LGL N  
Sbjct: 157 LKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYFFIDVILGLCNTF 216

Query: 187 VRSAFGIELEQPSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRPVRAA----LGRRRGA 246
           VR  FGIELE PSNEPYLATSL+DFWGRRWNLLVSNTLR++V+ PVRAA    LGRRR A
Sbjct: 217 VRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR-A 276

Query: 247 APAAVFATFVVSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKWRLH 306
           A  AVFATFVVSGLMHELLFYYVT AVP WEVT FFVLHGVCVVVEF  K A+GE WRLH
Sbjct: 277 AVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVVEFWAKMALGENWRLH 336

Query: 307 WAVSGPLTVAFVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEFVK 330
           WAV+ PLT+AFVV+T AWLFFPPLL+TGAV  ++ ECK+AA +VK
Sbjct: 337 WAVAAPLTLAFVVITAAWLFFPPLLKTGAVAAIIGECKAAAGYVK 380

[Close]

BLAST of MC11g1011 vs. ExPASy TrEMBL
Match: A0A6J1CCA4 (LOW QUALITY PROTEIN: probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Momordica charantia OX=3673 GN=LOC111010365 PE=3 SV=1)

HSP 1 Score: 650 bits (1677), Expect = 3.41e-235
Identity = 334/337 (99.11%), Postives = 335/337 (99.41%), Query Frame = 0


Query: 1   AAELHALIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLP-LLSGIVAFFI 60
           AAELHALIKVSLCVFAS+LYSYFVASKLPKGKFRLLSLLPIFPLFALLP LLSGIVAFFI
Sbjct: 2   AAELHALIKVSLCVFASLLYSYFVASKLPKGKFRLLSLLPIFPLFALLPXLLSGIVAFFI 61

Query: 61  TWLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQNPPKTLHPPPHLPDSPKLP 120
           TWLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQNPPKTLHPPPHLPDSPKLP
Sbjct: 62  TWLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQNPPKTLHPPPHLPDSPKLP 121

Query: 121 LNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIVRSAFGIELE 180
           LNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIVRSAFGIELE
Sbjct: 122 LNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIVRSAFGIELE 181

Query: 181 QPSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRPVRAALGRRRGAAPAAVFATFVVSGL 240
           QPSNEPYLATSLQDFWGRRWNLLVSNTLRY VYRPVRAALGRRRGAAPAAVFATFVVSGL
Sbjct: 182 QPSNEPYLATSLQDFWGRRWNLLVSNTLRYXVYRPVRAALGRRRGAAPAAVFATFVVSGL 241

Query: 241 MHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKWRLHWAVSGPLTVAFVVV 300
           MHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKWRLHWAVSGPLTVAFVVV
Sbjct: 242 MHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKWRLHWAVSGPLTVAFVVV 301

Query: 301 TGAWLFFPPLLRTGAVVGVLRECKSAAEFVKAQVSSV 336
           TGAWLFFPPLLRTGAVVGVLRECKSAAEFVKAQVSSV
Sbjct: 302 TGAWLFFPPLLRTGAVVGVLRECKSAAEFVKAQVSSV 338

[Close]

BLAST of MC11g1011 vs. ExPASy TrEMBL
Match: A0A6J1IZR7 (probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita maxima OX=3661 GN=LOC111480619 PE=3 SV=1)

HSP 1 Score: 467 bits (1201), Expect = 1.25e-162
Identity = 248/348 (71.26%), Postives = 275/348 (79.02%), Query Frame = 0


Query: 1   AAELHALIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLPL------LSGI 60
           AA+L+ L+K+S  V AS+LYSYF++SKLPKG  RLLSLLPI  L+ LLPL      L+GI
Sbjct: 2   AADLYTLLKISAAVLASLLYSYFISSKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGI 61

Query: 61  VAFFITWLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQN--------PPKTL 120
           VAFFITWLASFKLL F FDSGPL SDPPL FPLF SIAC+PIRIKQN        P K  
Sbjct: 62  VAFFITWLASFKLLLFCFDSGPLFSDPPLSFPLFVSIACYPIRIKQNQYDPDPQNPQKPT 121

Query: 121 HPPPHLPDSPKLPLNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLS 180
            PP    DSPKLPLNLPAK  LFA LVA DDFAQ+L  N  LCFYC  LY FIDVVLG+ 
Sbjct: 122 DPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNTMLCFYCVSLYLFIDVVLGIC 181

Query: 181 NAIVRSAFGIELEQPSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRPVRAAL----GRR 240
           N  VR  FGIELE PSNEPYLATSL+DFWGRRWNLLVSNTLR++V+ PVRAA+    GRR
Sbjct: 182 NTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVVGRR 241

Query: 241 RGAAPAAVFATFVVSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKW 300
           R AA  AVFATFVVSGLMHEL+FYYVT A+P WEVT FFVLHGVCVVVEF VK A+GE W
Sbjct: 242 R-AAVVAVFATFVVSGLMHELVFYYVTGAIPTWEVTGFFVLHGVCVVVEFWVKMALGENW 301

Query: 301 RLHWAVSGPLTVAFVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEFVK 330
           RLHWAV+GPLT+AFVV+T AWLFFPPLL+TG V  V+ ECK+AA +VK
Sbjct: 302 RLHWAVAGPLTLAFVVITAAWLFFPPLLKTGVVAAVIGECKAAAGYVK 348

[Close]

BLAST of MC11g1011 vs. ExPASy TrEMBL
Match: A0A6J1FJC3 (probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445989 PE=3 SV=1)

HSP 1 Score: 463 bits (1191), Expect = 4.02e-161
Identity = 248/347 (71.47%), Postives = 273/347 (78.67%), Query Frame = 0


Query: 2   AELHALIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLPL------LSGIV 61
           A+L+ L+K+S  V AS+LYSYF++SKLPKG  RLLSLLPI  L+ LLPL      L+GIV
Sbjct: 2   ADLYTLLKISAAVLASLLYSYFISSKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIV 61

Query: 62  AFFITWLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQNPPKTLHPPPHLP-- 121
           AFFITWLASFKLL F FDSGPL SDPPL FPLF SI+CFPIRIKQN P      P  P  
Sbjct: 62  AFFITWLASFKLLLFCFDSGPLFSDPPLSFPLFVSISCFPIRIKQNQPDPDPQNPQKPTD 121

Query: 122 ------DSPKLPLNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSN 181
                 DSPKL LNLPAK  LFA LVA DDFAQ+L  NA LCFYC  LY FIDV+LGL N
Sbjct: 122 PLLKTIDSPKLRLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYFFIDVILGLCN 181

Query: 182 AIVRSAFGIELEQPSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRPVRAA----LGRRR 241
             VR  FGIELE PSNEPYLATSL+DFWGRRWNLLVSNTLR++V+ PVRAA    LGRRR
Sbjct: 182 TFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR 241

Query: 242 GAAPAAVFATFVVSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKWR 301
            AA  AVFATFVVSGLMHELLFYYVT AVP WEVT FFVLHGVCVVVEF VK  +GE WR
Sbjct: 242 -AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVVEFWVKMVLGENWR 301

Query: 302 LHWAVSGPLTVAFVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEFVK 330
           LHWAV+GPLT+AFVV+T AWLFFPPLL+TGAV  ++ ECK+AA +VK
Sbjct: 302 LHWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAIIGECKAAAGYVK 347

[Close]

BLAST of MC11g1011 vs. ExPASy TrEMBL
Match: A0A0A0K8E9 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G145990 PE=3 SV=1)

HSP 1 Score: 382 bits (980), Expect = 4.28e-129
Identity = 210/338 (62.13%), Postives = 247/338 (73.08%), Query Frame = 0


Query: 2   AELHALIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLPLL------SGIV 61
           A+L AL+K+ L V AS+LYSYF++SKLPKG  RLLSLLPI  LF LLPLL      + IV
Sbjct: 6   ADLFALLKIFLSVLASLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLLVSSILFAAIV 65

Query: 62  AFFITWLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQNPPKTLHPPPHLPDS 121
           +  +TWL SFKLL FSF SGPL SDPPLPF LF S+A FPIRIK+   K    P    DS
Sbjct: 66  SSIVTWLTSFKLLLFSFHSGPLVSDPPLPFLLFVSLAFFPIRIKK---KESQKPI---DS 125

Query: 122 PKLPLNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIVRSAFG 181
           PKLP+N PAK  LF  L+A +D   SLP   KLCF C  LY FID+V+G  N I R  FG
Sbjct: 126 PKLPINFPAKVFLFTVLMAVED---SLPPYMKLCFNCVALYFFIDIVVGFFNTIARWVFG 185

Query: 182 IELEQPSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRPVRAAL----GRRRGAAPAAVF 241
           IE E+PSNEPYL+TSLQDFWGRRWN+LVSN LR TV+ PVR+A+    GRRR AA   VF
Sbjct: 186 IEFEEPSNEPYLSTSLQDFWGRRWNILVSNLLRLTVFIPVRSAMIDVAGRRRSAA-VGVF 245

Query: 242 ATFVVSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKWRLHWAVSGP 301
           A FVVSGLMHELLFYYV R  P+WEVT FFVL GVCV++EF++K AVGEK+R+H+AV   
Sbjct: 246 AVFVVSGLMHELLFYYVNRESPSWEVTGFFVLQGVCVLLEFQLKMAVGEKFRMHYAVCWV 305

Query: 302 LTVAFVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEFV 329
           LT+ FVVVT +WLFFPP+LR G V  +  +C++  E V
Sbjct: 306 LTMGFVVVTASWLFFPPVLRNGTVARLTGDCRAVLELV 333

[Close]

BLAST of MC11g1011 vs. ExPASy TrEMBL
Match: A0A6J1FP14 (probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445989 PE=3 SV=1)

HSP 1 Score: 378 bits (971), Expect = 1.65e-128
Identity = 210/292 (71.92%), Postives = 226/292 (77.40%), Query Frame = 0


Query: 2   AELHALIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLPL------LSGIV 61
           A+L+ L+K+S  V AS+LYSYF++SKLPKG  RLLSLLPI  L+ LLPL      L+GIV
Sbjct: 2   ADLYTLLKISAAVLASLLYSYFISSKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIV 61

Query: 62  AFFITWLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQNPPKTLHPPPHLP-- 121
           AFFITWLASFKLL F FDSGPL SDPPL FPLF SI+CFPIRIKQN P      P  P  
Sbjct: 62  AFFITWLASFKLLLFCFDSGPLFSDPPLSFPLFVSISCFPIRIKQNQPDPDPQNPQKPTD 121

Query: 122 ------DSPKLPLNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSN 181
                 DSPKL LNLPAK  LFA LVA DDFAQ+L  NA LCFYC  LY FIDV+LGL N
Sbjct: 122 PLLKTIDSPKLRLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYFFIDVILGLCN 181

Query: 182 AIVRSAFGIELEQPSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRPVRAA----LGRRR 241
             VR  FGIELE PSNEPYLATSL+DFWGRRWNLLVSNTLR++V+ PVRAA    LGRRR
Sbjct: 182 TFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR 241

Query: 242 GAAPAAVFATFVVSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVK 275
            AA  AVFATFVVSGLMHELLFYYVT AVP WEVT FFVLHGVCVVVEF VK
Sbjct: 242 -AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVVEFWVK 292

[Close]

BLAST of MC11g1011 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 248.8 bits (634), Expect = 6.1e-66
Identity = 153/341 (44.87%), Postives = 195/341 (57.18%), Query Frame = 0


Query: 3   ELHALIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLPL------LSGIVA 62
           EL  LIKV +    SI Y Y++  ++  G  R LS+ P+  LF +LPL      LS I A
Sbjct: 4   ELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSLITA 63

Query: 63  FFITWLASFKLLFFSFDSGPLASDPPLP--FPLFASIACFPIRIKQNPPKTLHPPPHLPD 122
           FF+TWLA+FKL+ FSFD GPL    P+P  FP F    CFPI+++QNP    H P     
Sbjct: 64  FFLTWLANFKLILFSFDKGPLI---PIPTNFPRFFCFTCFPIKVQQNPKSQNHLP----- 123

Query: 123 SPKLPLNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIVRSAF 182
                L    K  +FA L+    + Q+L     L  Y   LY  I+++L     +V  + 
Sbjct: 124 ----KLVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISL 183

Query: 183 GIELEQPSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRPVRAALGRRRGAAPA---AVF 242
           G +LE  SN+PYLATSLQDFWGRRWNL+V   LR  VY P+R    R+  +  A    + 
Sbjct: 184 GCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGIL 243

Query: 243 ATFVVSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKR--AVGEKWRLHWAVS 302
           A F+VSGL+HELLF+Y+ R +P  EVT FFVLHGVC  VE  VK+   V ++WRL   VS
Sbjct: 244 AAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSPGVS 303

Query: 303 GPLTVAFVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEFVK 331
             LTV FV VTG WLF P L R+G +     E     EF+K
Sbjct: 304 RVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIK 332

[Close]

BLAST of MC11g1011 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 234.2 bits (596), Expect = 1.5e-61
Identity = 148/340 (43.53%), Postives = 189/340 (55.59%), Query Frame = 0


Query: 7   LIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLPLLSGIVAF------FIT 66
           LI+V +    S+ Y Y+++SKL KG  RLLS+LP+  LF +LPL    V F      F++
Sbjct: 8   LIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCAISVLFLS 67

Query: 67  WLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQNPPKTLHPPPHLPDSPKLPL 126
           WLA+FKLL F+FD GPL   PP     F   AC PI+I+Q+P     P  H    PK   
Sbjct: 68  WLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQDPSPNAIPNLHPKPMPKWV- 127

Query: 127 NLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIVRSAFGIELEQ 186
            L  K L+   L+   ++   LP+   L  YC  +Y  +++VL    A+V +  G  +E 
Sbjct: 128 -LAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLLGCNIEP 187

Query: 187 PSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRPVRAALGR---RRGAAPAAVFATFVVS 246
             NEPYLATSLQDFW RRWNL+VS  LR TV+ PV+    R     GA  A V A+F VS
Sbjct: 188 VFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVMASFFVS 247

Query: 247 GLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKWRL--HWAVSGPLTVA 306
           GLMHELL++Y+ R  P WEVT FFVLHG     E  VKR    +W    H AVSG + + 
Sbjct: 248 GLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRT---QWLRPPHRAVSGLVVLT 307

Query: 307 FVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEFVKAQVSS 336
           FV VTG WLF   +LR       + EC    +  K   SS
Sbjct: 308 FVSVTGVWLFLAQVLRNNVHEKAIGECLLVLDLAKLFTSS 341

[Close]

BLAST of MC11g1011 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 234.2 bits (596), Expect = 1.5e-61
Identity = 152/342 (44.44%), Postives = 202/342 (59.06%), Query Frame = 0


Query: 3   ELHALIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLPL------LSGIVA 62
           EL   IK+ +    SI Y Y++++ +  G FRLLS+LP+  LF + PL       SG +A
Sbjct: 4   ELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSGCMA 63

Query: 63  FFITWLASFKLLFFSFDSGPLASDPPLPFPL--FASIACFPIRIKQNPPKTLHPPPHLPD 122
           FF++WLA+FKL+ FSFD GPL+   PLP  L  F  I CFPI+ +QNP            
Sbjct: 64  FFLSWLANFKLILFSFDQGPLS---PLPRTLSRFICITCFPIKPQQNPN---------IQ 123

Query: 123 SPKLPLNLPA-KALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIVRSA 182
           + K+P+ L A K ++F  L+   ++ Q L     L F    ++  +++V  L  A+V   
Sbjct: 124 NYKIPIWLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFIT 183

Query: 183 FGIELEQPSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRPVRAALGRRRGAAPA---AV 242
            G +LE  SNEPYLATSLQDFWGRRWNL+V   LR  VY P R    R+  +  A    V
Sbjct: 184 LGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGV 243

Query: 243 FATFVVSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRA--VGEKWRLHWAV 302
           FA F+VSG +HE+LF+Y+TR VP  EVT FF+LHGVC V E  VK++  V   WR+   V
Sbjct: 244 FAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTV 303

Query: 303 SGPLTVAFVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEFVK 331
           S  LTV FVVVT  W FF PL+R+G +  +  E     +FVK
Sbjct: 304 SRLLTVGFVVVTSGWFFF-PLIRSGIIERLASEALMCIDFVK 332

[Close]

BLAST of MC11g1011 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 233.4 bits (594), Expect = 2.6e-61
Identity = 144/340 (42.35%), Postives = 193/340 (56.76%), Query Frame = 0


Query: 3   ELHALIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLPL------LSGIVA 62
           EL +LIKV +    SI Y Y+ ++++  G FRLLS+LP+  LF +LPL       SG  A
Sbjct: 4   ELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSGSTA 63

Query: 63  FFITWLASFKLLFFSFDSGPLASDPPLPFPL--FASIACFPIRIKQNPPKTLHPPPHLPD 122
           FF++WLA+FKL+ FSFD GPL    P+P  L  F    CFPI+++QNP          P 
Sbjct: 64  FFLSWLANFKLILFSFDQGPLF---PVPSNLSRFVCFTCFPIKLQQNPK---------PQ 123

Query: 123 SPKLPLNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIVRSAF 182
           +         K   F  L+   ++ Q +     L  Y   +Y   +++L     ++  + 
Sbjct: 124 NQMPKWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISL 183

Query: 183 GIELEQPSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRPVRAALGRRRGAAPA---AVF 242
             +LE   NEPYL+TSLQDFWGRRWNL+V   LR  VY PVR   GR+  +  A    VF
Sbjct: 184 WCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVF 243

Query: 243 ATFVVSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRA-VGEKWRLHWAVSG 302
           A+F+VSG++HEL+F+Y TR  P  EVT FFVLHGVC   E   KR  +  +W++   VS 
Sbjct: 244 ASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSR 303

Query: 303 PLTVAFVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEFVK 331
            LTV FVV+TG WLFFP L R+G +  +  E      FVK
Sbjct: 304 LLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVK 331

[Close]

BLAST of MC11g1011 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 228.4 bits (581), Expect = 8.5e-60
Identity = 136/333 (40.84%), Postives = 194/333 (58.26%), Query Frame = 0


Query: 6   ALIKVSLCVFASILYSYFVASKLPKGKFRLLSLLPIFPLFALLPL------LSGIVAFFI 65
           + IK    V  S+ Y++F+A  +PKG  RL+   P+F +F ++P       L GI AFFI
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 66  TWLASFKLLFFSFDSGPLASD-PPLPFPLFASIACFPIRIKQNPPKTLHPPPHLPDSPKL 125
            WLA+FKLL F+   GPL+S+  PL  P+F +++C PI+I Q  PK      H   S + 
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKI-QLSPKPTKTHSH-EGSTEG 122

Query: 126 PLNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIVRSAFGIEL 185
           PL    KA+    ++ A +++  LP+   L  Y   +Y  ++++L  + A VR+   +EL
Sbjct: 123 PLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLEL 182

Query: 186 EQPSNEPYLATSLQDFWGRRWNLLVSNTLRYTVYRP---VRAALGRRRGAAPAAVFATFV 245
           E   N+PYLATSLQDFWGRRWNL+V+  LR TVY P   + + LG       AA F TFV
Sbjct: 183 EPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFSVLGPNYSQILAA-FGTFV 242

Query: 246 VSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKWRLHWAVSGPLTVA 305
           VSG+MHEL+F+Y+ R  P W++  FF+++G C  VE  +K+ +  +WR   A+S  LT+ 
Sbjct: 243 VSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLT 302

Query: 306 FVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEF 329
           FV+VT  WLF P   R   V   L E  +   F
Sbjct: 303 FVMVTALWLFLPEFNRCNIVEKALDEYAAIGAF 332

The following BLAST results are available for this feature:

BLAST of MC11g1011 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJ76	8.5e-65	44.87	Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX...	[more]
Q9FJ72	2.2e-60	43.53	Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX...	[more]
Q9FJ74	2.2e-60	44.44	Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX...	[more]
Q9FJ75	3.7e-60	42.35	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]
Q9SV07	1.2e-58	40.84	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]

BLAST of MC11g1011 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139430.1	7.05e-235	99.11	LOW QUALITY PROTEIN: probable long-chain-alcohol O-fatty-acyltransferase 5 [Momo...	[more]
XP_023525214.1	3.34e-165	72.70	probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita pepo subsp. pep...	[more]
XP_022981530.1	2.59e-162	71.26	probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita maxima]	[more]
XP_022940347.1	8.29e-161	71.47	probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X1 [Cucurbita mosc...	[more]
KAG6607778.1	1.25e-158	71.59	putative long-chain-alcohol O-fatty-acyltransferase 5, partial [Cucurbita argyro...	[more]

BLAST of MC11g1011 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CCA4	3.41e-235	99.11	LOW QUALITY PROTEIN: probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Mo...	[more]
A0A6J1IZR7	1.25e-162	71.26	probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita maxima OX=366...	[more]
A0A6J1FJC3	4.02e-161	71.47	probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X1 OS=Cucurbita mo...	[more]
A0A0A0K8E9	4.28e-129	62.13	MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G145990 PE=...	[more]
A0A6J1FP14	1.65e-128	71.92	probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X2 OS=Cucurbita mo...	[more]

BLAST of MC11g1011 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55340.1	6.1e-66	44.87	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55380.1	1.5e-61	43.53	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55360.1	1.5e-61	44.44	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55350.1	2.6e-61	42.35	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT3G51970.1	8.5e-60	40.84	acyl-CoA sterol acyl transferase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017088	Wax synthase, Magnoliopsida	PIRSF	PIRSF037006	Wax_synthase	coord: 1..336 e-value: 1.4E-115 score: 384.1
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 183..262 e-value: 1.8E-15 score: 57.0
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 2..334
None	No IPR available	PANTHER	PTHR31595:SF8	(MEMBRANE BOUND O-ACYL TRANSFERASE) FAMILY PROTEIN, PUTATIVE-RELATED	coord: 2..334

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g1011.1	MC11g1011.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity