MC11g0976 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g0976 vs. ExPASy Swiss-Prot
Match: Q9D0U1 (tRNA-uridine aminocarboxypropyltransferase 2 OS=Mus musculus OX=10090 GN=Dtwd2 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 1.4e-04
Identity = 30/109 (27.52%), Postives = 57/109 (52.29%), Query Frame = 0

Query: 240 SVLLFPSENAFTVDCGVDGSDFDVNNLIVLDGTWAKAKRMYNENPWLRLLPHLKLDLEKI 299
           +++L+P   A  ++  +  S    + +I++DGTW++AK ++ +N   RL   ++L     
Sbjct: 148 TLILYPGAEATNLEEFILDSPVYPSTIILIDGTWSQAKDIFYKNSLFRLPKQVQLKTSVC 207

Query: 300 SLYSDVRHQPKIGFLSTIESIVYALKLIGDNPEGLDSLLDVFESMIGDQ 349
           S Y  +R QP    LST+E    AL ++  N    ++LL   +++   Q
Sbjct: 208 SQYV-IRMQPTNRCLSTLECAAVALSILEKNNCIQETLLRPLQALCSFQ 255

BLAST of MC11g0976 vs. ExPASy Swiss-Prot
Match: Q47319 (tRNA-uridine aminocarboxypropyltransferase OS=Escherichia coli (strain K12) OX=83333 GN=tapT PE=1 SV=2)

HSP 1 Score: 48.1 bits (113), Expect = 2.4e-04
Identity = 23/63 (36.51%), Postives = 38/63 (60.32%), Query Frame = 0

Query: 267 IVLDGTWAKAKRMYNENPWLRLLPHLKLDLEKISLYSDVRHQPKIGFLSTIESIVYALKL 326
           I+LDGTW +A++M+ ++P+L  LP + +DL ++S Y  +R     G   T E  +  L +
Sbjct: 134 IMLDGTWPEARKMFRKSPYLDNLPVISVDLSRLSAYR-LREAQAEGQYCTAEVAIALLDM 193

Query: 327 IGD 330
            GD
Sbjct: 194 AGD 195

BLAST of MC11g0976 vs. ExPASy Swiss-Prot
Match: Q8NBA8 (tRNA-uridine aminocarboxypropyltransferase 2 OS=Homo sapiens OX=9606 GN=DTWD2 PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 5.3e-04
Identity = 29/109 (26.61%), Postives = 56/109 (51.38%), Query Frame = 0

Query: 240 SVLLFPSENAFTVDCGVDGSDFDVNNLIVLDGTWAKAKRMYNENPWLRLLPHLKLDLEKI 299
           +++L+P   A  ++  +  S    + +I++DGTW++AK ++ +N   R    ++L     
Sbjct: 148 TLILYPGAEAANLEEFILDSPVYPSTIIIIDGTWSQAKDIFYKNSLFRHPKQVQLKTSIS 207

Query: 300 SLYSDVRHQPKIGFLSTIESIVYALKLIGDNPEGLDSLLDVFESMIGDQ 349
           S Y  +R QP    LST+E    AL ++  N    ++LL   +++   Q
Sbjct: 208 SQYV-IRMQPTNRCLSTLECAAVALSILEKNNYIQETLLRPLQALCSFQ 255

BLAST of MC11g0976 vs. ExPASy Swiss-Prot
Match: Q4R7M4 (tRNA-uridine aminocarboxypropyltransferase 2 OS=Macaca fascicularis OX=9541 GN=DTWD2 PE=2 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 5.3e-04
Identity = 29/109 (26.61%), Postives = 56/109 (51.38%), Query Frame = 0

Query: 240 SVLLFPSENAFTVDCGVDGSDFDVNNLIVLDGTWAKAKRMYNENPWLRLLPHLKLDLEKI 299
           +++L+P   A  ++  +  S    + +I++DGTW++AK ++ +N   R    ++L     
Sbjct: 148 TLILYPGAEAANLEEFILDSPVYPSTIIIIDGTWSQAKDIFYKNSLFRHPKQVQLKTSIS 207

Query: 300 SLYSDVRHQPKIGFLSTIESIVYALKLIGDNPEGLDSLLDVFESMIGDQ 349
           S Y  +R QP    LST+E    AL ++  N    ++LL   +++   Q
Sbjct: 208 SQYV-IRMQPTNRCLSTLECAAVALSILEKNNYIQETLLRPLQALCSFQ 255

BLAST of MC11g0976 vs. NCBI nr
Match: XP_038897268.1 (uncharacterized protein LOC120085387 [Benincasa hispida])

HSP 1 Score: 532 bits (1371), Expect = 1.65e-187
Identity = 276/366 (75.41%), Postives = 312/366 (85.25%), Query Frame = 0

Query: 1   MILFRNRIRFGNLKDPLNRIQSPRQSKLLQTLAMADAPPNSRRPVCPSCSKPARICLCSR 60
           MI F  RIRFG+ K+PLNRI +  QSKLLQ LAMA+ PP+SRRPVCPSCSKPARICLCSR
Sbjct: 1   MIHFGFRIRFGSPKNPLNRIPNHLQSKLLQNLAMAEVPPSSRRPVCPSCSKPARICLCSR 60

Query: 61  LRNPGLENSVGVTILQHSLERKHPLNSARIAKLGLKNVQIATVSDVNFEARFTIR----- 120
           L++  LENSVGV ILQHSLE+ HPLNSARIAKLGLKNV+IATVSDVNFEARFTIR     
Sbjct: 61  LQSHSLENSVGVIILQHSLEKNHPLNSARIAKLGLKNVEIATVSDVNFEARFTIRLPEPN 120

Query: 121 -GTQDFDPEEKCCSTKGQNESRI-QIQNRDGFLLGDVIDSECIKPETPE-AVITATIGKY 180
              Q+FDP+ +C    GQN +R  QIQ ++G   G+ + ++       E A+IT TIGKY
Sbjct: 121 SAAQNFDPDVECSFRNGQNATRNPQIQAQNG---GESLVNKLTSTTADEGAIITTTIGKY 180

Query: 181 GVVNSFDHIWMQKPSFQKLKIDEVLASPAIRASVAKGFIVKKLQKRLLDGSQEMEEYAEF 240
           GVVNSFDHIWM +P+ Q+LKI+E+LASP IRAS+AKGFIVKKLQKR L GS+E+E+YAEF
Sbjct: 181 GVVNSFDHIWMHQPNLQELKINEILASPEIRASMAKGFIVKKLQKRQLHGSKELEQYAEF 240

Query: 241 EIEIPPKSVLLFPSENAFTVDCGVDGSDFDVNNLIVLDGTWAKAKRMYNENPWLRLLPHL 300
           EIE+PPKSVLLFPSENAFTV+  VDGSDFDVNNLIVLDGTWAKAKRMYNENPWLRLLPH+
Sbjct: 241 EIEVPPKSVLLFPSENAFTVNGDVDGSDFDVNNLIVLDGTWAKAKRMYNENPWLRLLPHM 300

Query: 301 KLDLEKISLYSDVRHQPKIGFLSTIESIVYALKLIGDNPEGLDSLLDVFESMIGDQRRCK 358
           KLDLEK+SLYS+VRHQPKIGFLSTIESIVYALK+IGD PEGLD LLDVFESMIGDQRRCK
Sbjct: 301 KLDLEKMSLYSEVRHQPKIGFLSTIESIVYALKVIGDQPEGLDDLLDVFESMIGDQRRCK 360

BLAST of MC11g0976 vs. NCBI nr
Match: KAG7024915.1 (hypothetical protein SDJN02_13735, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 516 bits (1328), Expect = 4.82e-181
Identity = 272/363 (74.93%), Postives = 303/363 (83.47%), Query Frame = 0

Query: 1   MILFRNRIRFGNLKDPLNRIQSPR-QSKLLQTLAMADAPPNSRRPVCPSCSKPARICLCS 60
           M+ FR RIRFGN K+PLNRI     Q KLLQTLAMAD PP+S R VCPSCSKPARICLCS
Sbjct: 1   MLHFRVRIRFGNSKNPLNRIPPNHLQFKLLQTLAMADVPPSSGRLVCPSCSKPARICLCS 60

Query: 61  RLRNPGLENSVGVTILQHSLERKHPLNSARIAKLGLKNVQIATVSDVNFEARFTIRGTQD 120
           RL+NP LENSVGV ILQHSLE+KHPLNS+RIAKLGLKNV+IATVSDVNFEARFTIR   +
Sbjct: 61  RLQNPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIATVSDVNFEARFTIR-LPE 120

Query: 121 FDPEEKCCSTKGQNESR---IQIQNRDGFLLGDVIDSECIKPETPEAVITATIGKYGVVN 180
            + +E+     GQN ++   IQ+QNRD  L    +D          A ITATIGKYGVVN
Sbjct: 121 LNSDEEFSFGNGQNAAQKPQIQVQNRDESL----VDKPTCTKSDEGAAITATIGKYGVVN 180

Query: 181 SFDHIWMQKPSFQKLKIDEVLASPAIRASVAKGFIVKKLQKRLLDGSQEMEEYAEFEIEI 240
           SFDHIWM +PS Q LKIDE+ ASP I+AS+AKGFIVKKLQKR +D S+E+EEYAEFEIE+
Sbjct: 181 SFDHIWMHQPSLQDLKIDEIFASPEIKASMAKGFIVKKLQKRQVDESKELEEYAEFEIEV 240

Query: 241 PPKSVLLFPSENAFTV-DCGVDGSDFDVNNLIVLDGTWAKAKRMYNENPWLRLLPHLKLD 300
           PPKSVLLFPS+NAFTV D  V GSDF VNNLIVLDGTWAKAKRMYNENPWLRLLPH+KLD
Sbjct: 241 PPKSVLLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLD 300

Query: 301 LEKISLYSDVRHQPKIGFLSTIESIVYALKLIGDNPEGLDSLLDVFESMIGDQRRCKDER 358
           LEK+SLYS+VR QPK GFLSTIESIVYALK+IGD+PEGLD LLDVFESMIGDQRRCKDER
Sbjct: 301 LEKMSLYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDER 358

BLAST of MC11g0976 vs. NCBI nr
Match: XP_023535710.1 (uncharacterized protein LOC111797060 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 514 bits (1325), Expect = 1.38e-180
Identity = 273/363 (75.21%), Postives = 302/363 (83.20%), Query Frame = 0

Query: 1   MILFRNRIRFGNLKDPLNRIQSPR-QSKLLQTLAMADAPPNSRRPVCPSCSKPARICLCS 60
           M+ FR RIRFGN K+PLNRI     Q KLLQTLAMAD PP++ R VCPSCSKPARICLCS
Sbjct: 1   MLHFRFRIRFGNSKNPLNRIPPIHLQFKLLQTLAMADVPPSTGRLVCPSCSKPARICLCS 60

Query: 61  RLRNPGLENSVGVTILQHSLERKHPLNSARIAKLGLKNVQIATVSDVNFEARFTIRGTQD 120
           RL+NP LENSVGV ILQHSLE+KHPLNS+RIAKLGLKNV IATVSDVNFEARFTIR   +
Sbjct: 61  RLQNPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVAIATVSDVNFEARFTIR-LPE 120

Query: 121 FDPEEKCCSTKGQNESR---IQIQNRDGFLLGDVIDSECIKPETPEAVITATIGKYGVVN 180
            + +E+     GQN ++   IQ+QNRD  L    +D          A ITATIGKYGVVN
Sbjct: 121 LNSDEEFSFGNGQNAAQKPQIQVQNRDESL----VDKPTCTKSDEGAAITATIGKYGVVN 180

Query: 181 SFDHIWMQKPSFQKLKIDEVLASPAIRASVAKGFIVKKLQKRLLDGSQEMEEYAEFEIEI 240
           SFDHIWM +PS Q LKIDEV ASP IRAS+AKGFIVKKLQKR +D S+E+EEYAEFEIE+
Sbjct: 181 SFDHIWMHQPSLQDLKIDEVFASPEIRASMAKGFIVKKLQKRQVDESKELEEYAEFEIEV 240

Query: 241 PPKSVLLFPSENAFTV-DCGVDGSDFDVNNLIVLDGTWAKAKRMYNENPWLRLLPHLKLD 300
           PPKSVLLFPS+NAFTV D  V GSDF VNNLIVLDGTWAKAKRMYNENPWLRLLPH+KLD
Sbjct: 241 PPKSVLLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLD 300

Query: 301 LEKISLYSDVRHQPKIGFLSTIESIVYALKLIGDNPEGLDSLLDVFESMIGDQRRCKDER 358
           LEK+SLYS+VR QPK GFLSTIESIVYALK+IGD+PEGLD LLDVFESMIGDQRRCKDER
Sbjct: 301 LEKMSLYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDER 358

BLAST of MC11g0976 vs. NCBI nr
Match: XP_022976533.1 (uncharacterized protein LOC111476900 isoform X1 [Cucurbita maxima])

HSP 1 Score: 514 bits (1323), Expect = 2.78e-180
Identity = 272/363 (74.93%), Postives = 302/363 (83.20%), Query Frame = 0

Query: 1   MILFRNRIRFGNLKDPLNRIQSPR-QSKLLQTLAMADAPPNSRRPVCPSCSKPARICLCS 60
           M+ FR RIRFGN K+PLNRI     Q KLLQTLAMAD PP+S R VCPSCSKPARICLCS
Sbjct: 1   MLHFRFRIRFGNSKNPLNRIPPNHLQFKLLQTLAMADVPPSSGRLVCPSCSKPARICLCS 60

Query: 61  RLRNPGLENSVGVTILQHSLERKHPLNSARIAKLGLKNVQIATVSDVNFEARFTIRGTQD 120
           RL++P LENSVGV ILQHSLE+KHPLNS+RIAKLGLKNV+IATVSDVNFEARFTIR   +
Sbjct: 61  RLQSPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIATVSDVNFEARFTIR-LPE 120

Query: 121 FDPEEKCCSTKGQNESR---IQIQNRDGFLLGDVIDSECIKPETPEAVITATIGKYGVVN 180
            + +E+     GQN ++   IQ+QNRD  L    +D          A ITATIGKYGVVN
Sbjct: 121 LNSDEEFSFGNGQNAAQKPQIQVQNRDESL----VDKPTCTKSDEGAAITATIGKYGVVN 180

Query: 181 SFDHIWMQKPSFQKLKIDEVLASPAIRASVAKGFIVKKLQKRLLDGSQEMEEYAEFEIEI 240
           SFDHIWM +PS Q LKIDE  ASP IRAS+AKGFIVKKLQKR +D S+E+EEYAEFEIE+
Sbjct: 181 SFDHIWMHQPSLQDLKIDETFASPEIRASMAKGFIVKKLQKRKVDESKELEEYAEFEIEV 240

Query: 241 PPKSVLLFPSENAFTV-DCGVDGSDFDVNNLIVLDGTWAKAKRMYNENPWLRLLPHLKLD 300
           PPKSVLLFPS+NAFTV D  V GSDF VNNLIVLDGTWAKAKRMYNENPWLRLLPH+KLD
Sbjct: 241 PPKSVLLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLD 300

Query: 301 LEKISLYSDVRHQPKIGFLSTIESIVYALKLIGDNPEGLDSLLDVFESMIGDQRRCKDER 358
           LEK+SLYS+VR QPK GFLSTIESIVYALK+IGD+PEGLD LLDVFESMIGDQRRCKDER
Sbjct: 301 LEKMSLYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDER 358

BLAST of MC11g0976 vs. NCBI nr
Match: XP_022936180.1 (uncharacterized protein LOC111442857 isoform X1 [Cucurbita moschata])

HSP 1 Score: 507 bits (1305), Expect = 1.52e-177
Identity = 269/363 (74.10%), Postives = 301/363 (82.92%), Query Frame = 0

Query: 1   MILFRNRIRFGNLKDPLNRIQSPR-QSKLLQTLAMADAPPNSRRPVCPSCSKPARICLCS 60
           M+ FR RIRFGN K+PLNR      Q KLLQTLAMAD PP++ R VCPSCSKPARICLCS
Sbjct: 1   MLHFRFRIRFGNSKNPLNRFPPNHLQFKLLQTLAMADVPPSTGRLVCPSCSKPARICLCS 60

Query: 61  RLRNPGLENSVGVTILQHSLERKHPLNSARIAKLGLKNVQIATVSDVNFEARFTIRGTQD 120
           RL NP LENSVGV ILQHSLE+KHPLNS+RIAKLGLKNV+IA VSDVNFEARFTIR   +
Sbjct: 61  RLHNPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIAPVSDVNFEARFTIR-LPE 120

Query: 121 FDPEEKCCSTKGQNESR---IQIQNRDGFLLGDVIDSECIKPETPEAVITATIGKYGVVN 180
            + +E+     GQN ++   IQ+QNRD  L+G      C K +   A ITATIGKYGVVN
Sbjct: 121 LNSDEEFSFGNGQNAAQKPQIQVQNRDESLVGK---PTCTKSDEG-AAITATIGKYGVVN 180

Query: 181 SFDHIWMQKPSFQKLKIDEVLASPAIRASVAKGFIVKKLQKRLLDGSQEMEEYAEFEIEI 240
           SF HIWM +PS Q LKIDE+ ASP IR+S+AKGFIVKKLQKR +D S+E+EEYAEFEIE+
Sbjct: 181 SFKHIWMHQPSLQDLKIDEIFASPEIRSSMAKGFIVKKLQKRQVDESKELEEYAEFEIEV 240

Query: 241 PPKSVLLFPSENAFTV-DCGVDGSDFDVNNLIVLDGTWAKAKRMYNENPWLRLLPHLKLD 300
           PPKSVLLFPS+NAFTV D  V  SDF VNNLIVLDGTWAKAKRMYNENPWLRLLPH+KLD
Sbjct: 241 PPKSVLLFPSDNAFTVNDDDVHSSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLD 300

Query: 301 LEKISLYSDVRHQPKIGFLSTIESIVYALKLIGDNPEGLDSLLDVFESMIGDQRRCKDER 358
           LEK+SLYS+VR QPK GFLSTIESIVYALK+IGD+PEGLD LLDVFESMIGDQRRCKDER
Sbjct: 301 LEKMSLYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDER 358

BLAST of MC11g0976 vs. ExPASy TrEMBL
Match: A0A6J1IMF8 (tRNA-uridine aminocarboxypropyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111476900 PE=4 SV=1)

HSP 1 Score: 514 bits (1323), Expect = 1.34e-180
Identity = 272/363 (74.93%), Postives = 302/363 (83.20%), Query Frame = 0

Query: 1   MILFRNRIRFGNLKDPLNRIQSPR-QSKLLQTLAMADAPPNSRRPVCPSCSKPARICLCS 60
           M+ FR RIRFGN K+PLNRI     Q KLLQTLAMAD PP+S R VCPSCSKPARICLCS
Sbjct: 1   MLHFRFRIRFGNSKNPLNRIPPNHLQFKLLQTLAMADVPPSSGRLVCPSCSKPARICLCS 60

Query: 61  RLRNPGLENSVGVTILQHSLERKHPLNSARIAKLGLKNVQIATVSDVNFEARFTIRGTQD 120
           RL++P LENSVGV ILQHSLE+KHPLNS+RIAKLGLKNV+IATVSDVNFEARFTIR   +
Sbjct: 61  RLQSPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIATVSDVNFEARFTIR-LPE 120

Query: 121 FDPEEKCCSTKGQNESR---IQIQNRDGFLLGDVIDSECIKPETPEAVITATIGKYGVVN 180
            + +E+     GQN ++   IQ+QNRD  L    +D          A ITATIGKYGVVN
Sbjct: 121 LNSDEEFSFGNGQNAAQKPQIQVQNRDESL----VDKPTCTKSDEGAAITATIGKYGVVN 180

Query: 181 SFDHIWMQKPSFQKLKIDEVLASPAIRASVAKGFIVKKLQKRLLDGSQEMEEYAEFEIEI 240
           SFDHIWM +PS Q LKIDE  ASP IRAS+AKGFIVKKLQKR +D S+E+EEYAEFEIE+
Sbjct: 181 SFDHIWMHQPSLQDLKIDETFASPEIRASMAKGFIVKKLQKRKVDESKELEEYAEFEIEV 240

Query: 241 PPKSVLLFPSENAFTV-DCGVDGSDFDVNNLIVLDGTWAKAKRMYNENPWLRLLPHLKLD 300
           PPKSVLLFPS+NAFTV D  V GSDF VNNLIVLDGTWAKAKRMYNENPWLRLLPH+KLD
Sbjct: 241 PPKSVLLFPSDNAFTVNDDDVHGSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLD 300

Query: 301 LEKISLYSDVRHQPKIGFLSTIESIVYALKLIGDNPEGLDSLLDVFESMIGDQRRCKDER 358
           LEK+SLYS+VR QPK GFLSTIESIVYALK+IGD+PEGLD LLDVFESMIGDQRRCKDER
Sbjct: 301 LEKMSLYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDER 358

BLAST of MC11g0976 vs. ExPASy TrEMBL
Match: A0A6J1FCX1 (tRNA-uridine aminocarboxypropyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111442857 PE=4 SV=1)

HSP 1 Score: 507 bits (1305), Expect = 7.36e-178
Identity = 269/363 (74.10%), Postives = 301/363 (82.92%), Query Frame = 0

Query: 1   MILFRNRIRFGNLKDPLNRIQSPR-QSKLLQTLAMADAPPNSRRPVCPSCSKPARICLCS 60
           M+ FR RIRFGN K+PLNR      Q KLLQTLAMAD PP++ R VCPSCSKPARICLCS
Sbjct: 1   MLHFRFRIRFGNSKNPLNRFPPNHLQFKLLQTLAMADVPPSTGRLVCPSCSKPARICLCS 60

Query: 61  RLRNPGLENSVGVTILQHSLERKHPLNSARIAKLGLKNVQIATVSDVNFEARFTIRGTQD 120
           RL NP LENSVGV ILQHSLE+KHPLNS+RIAKLGLKNV+IA VSDVNFEARFTIR   +
Sbjct: 61  RLHNPSLENSVGVIILQHSLEKKHPLNSSRIAKLGLKNVEIAPVSDVNFEARFTIR-LPE 120

Query: 121 FDPEEKCCSTKGQNESR---IQIQNRDGFLLGDVIDSECIKPETPEAVITATIGKYGVVN 180
            + +E+     GQN ++   IQ+QNRD  L+G      C K +   A ITATIGKYGVVN
Sbjct: 121 LNSDEEFSFGNGQNAAQKPQIQVQNRDESLVGK---PTCTKSDEG-AAITATIGKYGVVN 180

Query: 181 SFDHIWMQKPSFQKLKIDEVLASPAIRASVAKGFIVKKLQKRLLDGSQEMEEYAEFEIEI 240
           SF HIWM +PS Q LKIDE+ ASP IR+S+AKGFIVKKLQKR +D S+E+EEYAEFEIE+
Sbjct: 181 SFKHIWMHQPSLQDLKIDEIFASPEIRSSMAKGFIVKKLQKRQVDESKELEEYAEFEIEV 240

Query: 241 PPKSVLLFPSENAFTV-DCGVDGSDFDVNNLIVLDGTWAKAKRMYNENPWLRLLPHLKLD 300
           PPKSVLLFPS+NAFTV D  V  SDF VNNLIVLDGTWAKAKRMYNENPWLRLLPH+KLD
Sbjct: 241 PPKSVLLFPSDNAFTVNDDDVHSSDFHVNNLIVLDGTWAKAKRMYNENPWLRLLPHMKLD 300

Query: 301 LEKISLYSDVRHQPKIGFLSTIESIVYALKLIGDNPEGLDSLLDVFESMIGDQRRCKDER 358
           LEK+SLYS+VR QPK GFLSTIESIVYALK+IGD+PEGLD LLDVFESMIGDQRRCKDER
Sbjct: 301 LEKMSLYSEVRRQPKTGFLSTIESIVYALKVIGDHPEGLDDLLDVFESMIGDQRRCKDER 358

BLAST of MC11g0976 vs. ExPASy TrEMBL
Match: A0A1S3BZW7 (tRNA-uridine aminocarboxypropyltransferase OS=Cucumis melo OX=3656 GN=LOC103495005 PE=4 SV=1)

HSP 1 Score: 506 bits (1302), Expect = 2.18e-177
Identity = 264/365 (72.33%), Postives = 295/365 (80.82%), Query Frame = 0

Query: 1   MILFRNRIRFGNLKDPLNRIQSPRQSKLLQTLAMADAPPNSRRPVCPSCSKPARICLCSR 60
           MI F  RIRFGN K  LN + +  QSKL   LAMAD  P+SRRPVCPSCSKPARICLCSR
Sbjct: 1   MIHFPFRIRFGNPKSLLNPLPNRLQSKLFNNLAMADVLPSSRRPVCPSCSKPARICLCSR 60

Query: 61  LRNPGLENSVGVTILQHSLERKHPLNSARIAKLGLKNVQIATVSDVNFEARFTIR----- 120
            R+P LENSVG+ ILQHS E+ HPLNSARIAKLGLKNV IATVSDVNFEA FTIR     
Sbjct: 61  FRSPSLENSVGIIILQHSSEKNHPLNSARIAKLGLKNVDIATVSDVNFEAHFTIRLPEPN 120

Query: 121 -GTQDFDPEEKCCSTKGQNES-RIQIQNRDGFLLGDVIDSECIKPETPEAVITATIGKYG 180
              Q+ DP+ +C    G N + + QIQ+        ++D          A I+ATIGKYG
Sbjct: 121 SAAQNLDPDAECSFRNGHNATQKSQIQDE------SIVDKLNSTKANKGAAISATIGKYG 180

Query: 181 VVNSFDHIWMQKPSFQKLKIDEVLASPAIRASVAKGFIVKKLQKRLLDGSQEMEEYAEFE 240
           VVNSFDHIWMQ+P FQ+LKI+EVLASP IRAS+AKGFIVKKLQKR L  S+ +EEYAEFE
Sbjct: 181 VVNSFDHIWMQQPGFQELKINEVLASPEIRASLAKGFIVKKLQKRKLVESKGLEEYAEFE 240

Query: 241 IEIPPKSVLLFPSENAFTVDCGVDGSDFDVNNLIVLDGTWAKAKRMYNENPWLRLLPHLK 300
           I++PPKSVLLFPSENAFTV  GVDGSDF +N+LIVLDGTWAKAKRMYNENPWLRLLPH+K
Sbjct: 241 IQVPPKSVLLFPSENAFTVSGGVDGSDFYINHLIVLDGTWAKAKRMYNENPWLRLLPHMK 300

Query: 301 LDLEKISLYSDVRHQPKIGFLSTIESIVYALKLIGDNPEGLDSLLDVFESMIGDQRRCKD 358
           LDLE +SLYS+VRHQPKIGFLSTIESIVYALKLIGD+PEGLD LLDVFESMIGDQR+CKD
Sbjct: 301 LDLEMMSLYSEVRHQPKIGFLSTIESIVYALKLIGDHPEGLDDLLDVFESMIGDQRKCKD 359

BLAST of MC11g0976 vs. ExPASy TrEMBL
Match: A0A0A0LD09 (tRNA-uridine aminocarboxypropyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G851850 PE=4 SV=1)

HSP 1 Score: 494 bits (1272), Expect = 1.18e-172
Identity = 260/365 (71.23%), Postives = 290/365 (79.45%), Query Frame = 0

Query: 1   MILFRNRIRFGNLKDPLNRIQSPRQSKLLQTLAMADAPPNSRRPVCPSCSKPARICLCSR 60
           MI F   IRFGN K+ LN   +  QSKL   L MAD  P+SRRPVCPSCSKPARICLCSR
Sbjct: 12  MIHFSFPIRFGNPKNLLNPQPNRHQSKLFHNLPMADVLPSSRRPVCPSCSKPARICLCSR 71

Query: 61  LRNPGLENSVGVTILQHSLERKHPLNSARIAKLGLKNVQIATVSDVNFEARFTIR----- 120
            R+  +ENSVGV ILQHSLE+ HPLNSARI KLGLKNV+IATVSDVNFEARFTIR     
Sbjct: 72  FRSSSVENSVGVIILQHSLEKNHPLNSARIVKLGLKNVEIATVSDVNFEARFTIRLPEPN 131

Query: 121 -GTQDFDPEEKCCSTKGQNE-SRIQIQNRDGFLLGDVIDSECIKPETPEAVITATIGKYG 180
              Q+ DP+ +C    G     + QIQ+      G ++D          A I+ TIGK+G
Sbjct: 132 SAAQNLDPDIECSFRNGHGTIQKPQIQD------GSIVDKLNCTKTNEGAAISVTIGKHG 191

Query: 181 VVNSFDHIWMQKPSFQKLKIDEVLASPAIRASVAKGFIVKKLQKRLLDGSQEMEEYAEFE 240
           VVNSFDHIWMQ P FQ+LKI+E LASP IRAS+AKGFIVKK+QKR L  S+ +EEYAEFE
Sbjct: 192 VVNSFDHIWMQLPGFQELKINEFLASPEIRASLAKGFIVKKMQKRQLVESKGLEEYAEFE 251

Query: 241 IEIPPKSVLLFPSENAFTVDCGVDGSDFDVNNLIVLDGTWAKAKRMYNENPWLRLLPHLK 300
           I++PPKSVLLFPSENAFTV  GVDGSDF +NNLIVLDGTWAKAKRMYNENPWLRLLPH+K
Sbjct: 252 IQVPPKSVLLFPSENAFTVSGGVDGSDFYINNLIVLDGTWAKAKRMYNENPWLRLLPHMK 311

Query: 301 LDLEKISLYSDVRHQPKIGFLSTIESIVYALKLIGDNPEGLDSLLDVFESMIGDQRRCKD 358
           LDLE +SLYS+VRHQPKIGFLSTIESIVYALKLIGD+PEGLD LLDVFESMIGDQRRCKD
Sbjct: 312 LDLEIMSLYSEVRHQPKIGFLSTIESIVYALKLIGDHPEGLDDLLDVFESMIGDQRRCKD 370

BLAST of MC11g0976 vs. ExPASy TrEMBL
Match: A0A5A7T5E2 (tRNA-uridine aminocarboxypropyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold110G001820 PE=4 SV=1)

HSP 1 Score: 479 bits (1234), Expect = 1.47e-167
Identity = 246/332 (74.10%), Postives = 275/332 (82.83%), Query Frame = 0

Query: 34  MADAPPNSRRPVCPSCSKPARICLCSRLRNPGLENSVGVTILQHSLERKHPLNSARIAKL 93
           MAD  P+SRRPVCPSCSKPARICLCSR R+P LENSVG+ ILQHS E+ HPLNSARIAKL
Sbjct: 1   MADVLPSSRRPVCPSCSKPARICLCSRFRSPSLENSVGIIILQHSSEKNHPLNSARIAKL 60

Query: 94  GLKNVQIATVSDVNFEARFTIR------GTQDFDPEEKCCSTKGQNES-RIQIQNRDGFL 153
           GLKNV IATVSDVNFEA FTIR        Q+ DP+ +C    G N + + QIQ+     
Sbjct: 61  GLKNVDIATVSDVNFEAHFTIRLPEPNSAAQNLDPDAECSFRNGHNATQKSQIQDE---- 120

Query: 154 LGDVIDSECIKPETPEAVITATIGKYGVVNSFDHIWMQKPSFQKLKIDEVLASPAIRASV 213
              ++D          A I+ATIGKYGVVNSFDHIWMQ+P FQ+LKI+EVLASP IRAS+
Sbjct: 121 --SIVDKLNSTKANKGAAISATIGKYGVVNSFDHIWMQQPGFQELKINEVLASPEIRASL 180

Query: 214 AKGFIVKKLQKRLLDGSQEMEEYAEFEIEIPPKSVLLFPSENAFTVDCGVDGSDFDVNNL 273
           AKGFIVKKLQKR L  S+ +EEYAEFEI++PPKSVLLFPSENAFTV  GVDGSDF +N+L
Sbjct: 181 AKGFIVKKLQKRKLVESKGLEEYAEFEIQVPPKSVLLFPSENAFTVSGGVDGSDFYINHL 240

Query: 274 IVLDGTWAKAKRMYNENPWLRLLPHLKLDLEKISLYSDVRHQPKIGFLSTIESIVYALKL 333
           IVLDGTWAKAKRMYNENPWLRLLPH+KLDLE +SLYS+VRHQPKIGFLSTIESIVYALKL
Sbjct: 241 IVLDGTWAKAKRMYNENPWLRLLPHMKLDLEMMSLYSEVRHQPKIGFLSTIESIVYALKL 300

Query: 334 IGDNPEGLDSLLDVFESMIGDQRRCKDERLKM 358
           IGD+PEGLD LLDVFESMIGDQR+CKDERLK+
Sbjct: 301 IGDHPEGLDDLLDVFESMIGDQRKCKDERLKI 326

BLAST of MC11g0976 vs. TAIR 10
Match: AT5G54880.1 (DTW domain-containing protein )

HSP 1 Score: 279.6 bits (714), Expect = 3.4e-75
Identity = 162/380 (42.63%), Postives = 221/380 (58.16%), Query Frame = 0

Query: 19  RIQSPRQSKLLQTLAMADAPPNSRRPVCPSCSKPARICLCSRLRNPGLENSVGVTILQHS 78
           R Q  R SK L+          ++RP CPSC KP+++CLC ++R+P  +N V +TILQHS
Sbjct: 29  RCQGVRVSKTLKM--------GTKRPTCPSCDKPSQLCLCKKMRSPCFDNQVSITILQHS 88

Query: 79  LERKHPLNSARIAKLGLKNVQIATVSDVNFEARFTIR----GTQDFDPEEKCCSTKGQNE 138
           LERKH LNS RIA+LGLKNV + TV DV+ EA F IR    G    D        + +N 
Sbjct: 89  LERKHALNSTRIARLGLKNVGVTTVFDVHDEAEFLIRVIGSGCSKIDTNHLDSEYRVENV 148

Query: 139 SRIQIQNRDGFLLGDVIDSECIKPET---------------------------------- 198
           + +++   D F LG   + E ++ E                                   
Sbjct: 149 ASLELD--DSFKLGSSGNVENLESEKNVVLVDHGSLEIAESLNLSQHLSEISYRDSRVRD 208

Query: 199 -----PEAVITATIGKYGVVNSFDHIWMQKPSFQKLKIDEVLASPAIRASVAKGFIVKKL 258
                 E +I   + K+GV+++  H  M + + + L  D +LASPA    +AKGF+V K 
Sbjct: 209 KIGSYEEDLIKICMKKHGVISNVSHSLMLETNVKVLSFDHILASPAAMDVLAKGFMVTKF 268

Query: 259 QKRLLDGSQEMEEYAEFEIEIPPKSVLLFPSENAFTVDCGVDGSDFDVNNLIVLDGTWAK 318
                +G Q      EFE+E+PP S LLFPSE +  ++   +  +  V NLIVLDGTW+K
Sbjct: 269 S----EGKQ------EFELEVPPGSALLFPSEESVKINYLKEKEELKVRNLIVLDGTWSK 328

Query: 319 AKRMYNENPWLRLLP-HLKLDLEKISLYSDVRHQPKIGFLSTIESIVYALKLIGDNPEGL 355
           A+R+Y ENPWL+LL  H+KL++E  SLY +VR QP+ G LSTIESIVYA+K IG++PEGL
Sbjct: 329 ARRIYLENPWLKLLRCHVKLEIEGTSLYKEVRRQPRAGCLSTIESIVYAMKEIGEDPEGL 388