MC11g0972 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g0972 vs. ExPASy Swiss-Prot
Match: Q6WG30 (Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2)

HSP 1 Score: 340.1 bits (871), Expect = 3.7e-92
Identity = 178/446 (39.91%), Postives = 275/446 (61.66%), Query Frame = 0

Query: 1   LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK 60
           LPPG LG+P+IG+S   LRA+RSNS EQ+   RV K+G V K +L G PTV + G   N+
Sbjct: 52  LPPGKLGIPFIGESFIFLRALRSNSLEQFFDERVKKFGLVFKTSLIGHPTVVLCGPAGNR 111

Query: 61  AVFTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMDG 120
            + + EE  +         +++GE ++    GEDH  +R AL  F  P +L+ Y+GKM+ 
Sbjct: 112 LILSNEEKLVQMSWPAQFMKLMGENSVATRRGEDHIVMRSALAGFFGPGALQSYIGKMNT 171

Query: 121 EIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWSV 180
           EI+ H+   W    EV VLPL++ L F+I   L F I +   +  +    +T++ G +++
Sbjct: 172 EIQSHINEKWKGKDEVNVLPLVRELVFNISAILFFNIYDKQEQDRLHKLLETILVGSFAL 231

Query: 181 PINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQEEEQQDLVTCLLSMKNKDKEQ 240
           PI+LP   +  +L+  AK+ +++  L+K+++E ++        QDL++ LL+ ++ DK  
Sbjct: 232 PIDLPGFGFHRALQGRAKLNKIMLSLIKKRKEDLQ-SGSATATQDLLSVLLTFRD-DKGT 291

Query: 241 ALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGEPL 300
            L+ DEI+ N   L+ A +DTTT  + L+ ++L SNP  Y  V+QE  EI  +KE GE +
Sbjct: 292 PLTNDEILDNFSSLLHASYDTTTSPMALIFKLLSSNPECYQKVVQEQLEILSNKEEGEEI 351

Query: 301 SWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHLDD 360
           +W+D+  MKYTW+VA ETLR+FPPVFG FR A+ DI++ GYTIPKGW++ W    TH  D
Sbjct: 352 TWKDLKAMKYTWQVAQETLRMFPPVFGTFRKAITDIQYDGYTIPKGWKLLWTTYSTHPKD 411

Query: 361 TIFADPHKFEPNRFDKQ-TLIPPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQFT-- 420
             F +P KF P+RFD++   + P  F+ FGGG R C G EF+K+E L+ +H+ +  F+  
Sbjct: 412 LYFNEPEKFMPSRFDQEGKHVAPYTFLPFGGGQRSCVGWEFSKMEILLFVHHFVKTFSSY 471

Query: 421 WKLCSEEFLTRDP-TLMPNKGLPIQI 443
             +  +E ++ DP   +P+KG  I++
Sbjct: 472 TPVDPDEKISGDPLPPLPSKGFSIKL 495

BLAST of MC11g0972 vs. ExPASy Swiss-Prot
Match: Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 1.6e-87
Identity = 171/444 (38.51%), Postives = 266/444 (59.91%), Query Frame = 0

Query: 1   LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK 60
           +PPG  G P IG++L  L   R  +   +   R  KYG V   +L G PTV       N+
Sbjct: 51  IPPGTFGWPLIGETLEFLGCQRKGNPRDFFDSRTQKYGNVFTTSLVGHPTVVFCSPEGNR 110

Query: 61  AVFTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMDG 120
            +F+ E   + N    S+  +     +T + G+D KR+R  L++FL+P++L+++VG++D 
Sbjct: 111 FLFSNENKLVVNSWPSSVGNLFRSSLITTV-GDDAKRLRRILMTFLRPEALREFVGRVDS 170

Query: 121 EIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWSV 180
             ++HL  +W    EV  LPL+K  TF + C L   I        +   F   V G+  +
Sbjct: 171 MTKRHLAEHWIGKDEVMALPLLKRYTFSLACDLFASINTKDDLDRLWLHFMVFVKGVMQI 230

Query: 181 PINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQEEEQQDLVTCLLSMKNKDKE- 240
           PI+LP TRY+ +  A+  ++Q L  ++ E++  +E  +   E QDL++ LLS  N D++ 
Sbjct: 231 PIDLPGTRYNKTKHAANAIRQQLGSIINERKIALEAGNASPE-QDLLSFLLS--NVDEQG 290

Query: 241 QALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGEP 300
           ++L+++EI  NI++L+ AGHDT++  +T++++ L  NP  Y  VL+E   IA SKE G+ 
Sbjct: 291 ESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAENPHCYEEVLREQLNIAGSKEEGQL 350

Query: 301 LSWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHLD 360
           L WED+ +MKY+WRVA E LRLFP V G FR A+++  + G+TIPKGW++ W    TH  
Sbjct: 351 LEWEDLQRMKYSWRVAQEALRLFPAVQGSFRKAIKEFIYDGFTIPKGWKLHWTVNSTHQK 410

Query: 361 DTIFADPHKFEPNRFDKQTLIPPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQFTWK 420
              F++P KF+P+RF+ +   PP  F+ FGGGPR+CPG+EFA++E LI +H ++  F W 
Sbjct: 411 SEYFSNPEKFDPSRFEGEG-PPPYTFVPFGGGPRMCPGNEFARMEILIFLHNIVKNFNWN 470

Query: 421 LCSE-EFLTRDPTLMPNKGLPIQI 443
           L +  E +  DP   P  GLPI++
Sbjct: 471 LVNPLEKVIVDPMPAPVNGLPIKL 489

BLAST of MC11g0972 vs. ExPASy Swiss-Prot
Match: Q84KI1 (Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 8.0e-87
Identity = 168/446 (37.67%), Postives = 262/446 (58.74%), Query Frame = 0

Query: 1   LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK 60
           LPPG LG P+IG+SL  L+A+RSN+ EQ+L  RV  +G V K +L G PTV + G   N+
Sbjct: 50  LPPGKLGYPFIGESLLFLKALRSNTVEQFLDERVKNFGNVFKTSLIGHPTVVLCGPAGNR 109

Query: 61  AVFTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMDG 120
            +   EE  +     +S  +++GE+++T   GE H  +R AL  F  P +L+KY+G+M  
Sbjct: 110 LILANEEKLVQMSWPKSSMKLMGEKSITAKRGEGHMIIRSALQGFFSPGALQKYIGQMSK 169

Query: 121 EIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWSV 180
            I  H+   W  N +V+V+ L+  L FDI   L F I E   R+ +    + +  G+ +V
Sbjct: 170 TIENHINEKWKGNDQVSVVALVGDLVFDISACLFFNINEKHERERLFELLEIIAVGVLAV 229

Query: 181 PINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQEEEQQDLVTCLLSMKNKDKEQ 240
           P++LP   Y  +L+A +K+  +L  L+ EKR+           QDL++  L+ K+ D+  
Sbjct: 230 PVDLPGFAYHRALQARSKLNAILSGLI-EKRKMDLSSGLATSNQDLLSVFLTFKD-DRGN 289

Query: 241 ALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGEPL 300
             S++EI+ N   L+   +DTT   +  + ++L SNP  Y  V+QE   I  +K  G+ +
Sbjct: 290 PCSDEEILDNFSGLLHGSYDTTVSAMACVFKLLSSNPECYEKVVQEQLGILSNKLEGDEI 349

Query: 301 SWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHLDD 360
           +W+DV  MKYTW+V  ETLRL+P +FG FR A+ DI + GY IPKGW++ W    TH  +
Sbjct: 350 TWKDVKSMKYTWQVVQETLRLYPSIFGSFRQAITDIHYNGYIIPKGWKLLWTPYTTHPKE 409

Query: 361 TIFADPHKFEPNRFDKQ-TLIPPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQFT-- 420
             F++P KF P+RFD++  L+ P  F+ FGGG R CPG EF+K+E L+++H+ +  F+  
Sbjct: 410 MYFSEPEKFLPSRFDQEGKLVAPYTFLPFGGGQRSCPGWEFSKMEILLSVHHFVKTFSTF 469

Query: 421 WKLCSEEFLTRDPTL-MPNKGLPIQI 443
             +   E + RD    +P+ G  +++
Sbjct: 470 TPVDPAEIIARDSLCPLPSNGFSVKL 493

BLAST of MC11g0972 vs. ExPASy Swiss-Prot
Match: Q50EK0 (Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.4e-86
Identity = 169/444 (38.06%), Postives = 266/444 (59.91%), Query Frame = 0

Query: 1   LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK 60
           +PPG  G P IG++L  L   R  +   +   R  KYG V   +L G PTV       N+
Sbjct: 51  IPPGTFGWPLIGETLEFLGCQRKGNPRDFFDSRTQKYGNVFTTSLVGHPTVVFCSPEGNR 110

Query: 61  AVFTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMDG 120
            +F+ E   + N    S+  +     +T + G+D KR+R  L++FL+P++L+++VG++D 
Sbjct: 111 FLFSNENKLVVNSWPSSVGNLFRSSLITTV-GDDAKRLRRILMTFLRPEALREFVGRVDS 170

Query: 121 EIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWSV 180
             ++HL  +W    EVT LPL+K  TF + C L   I        +   F   V G+  +
Sbjct: 171 MTKRHLAEHWIGKDEVTALPLLKRYTFSLACDLFASINNKDDLGRLWLHFMVFVKGVMQI 230

Query: 181 PINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQEEEQQDLVTCLLSMKNKDKE- 240
           PI+LP TRY+ +  A+  ++Q L  ++ E++  +E  +   E QDL++ LLS  N D++ 
Sbjct: 231 PIDLPGTRYNKAKHAANAIRQQLGSIINERKIGLEAGNASPE-QDLLSFLLS--NVDEQG 290

Query: 241 QALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGEP 300
           ++L+++EI  NI++L+ AGHDT++  +T++++ L  NP  Y  VL+E  +IA SKE G+ 
Sbjct: 291 ESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAENPHCYEEVLREQLDIAGSKEAGQL 350

Query: 301 LSWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHLD 360
           L WED+ +MKY+WRVA E LRLFP   G FR A+++  + G+TIPKGW+++W    TH  
Sbjct: 351 LEWEDLQRMKYSWRVAQEALRLFPAAQGSFRKAIKEFIYDGFTIPKGWKMYWTVNSTHRK 410

Query: 361 DTIFADPHKFEPNRFDKQTLIPPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQFTWK 420
              F++P  F+P+RF+ +   PP  F+ FGGGPR+CPG+EFA+LE L+ +H ++    W 
Sbjct: 411 SEYFSNPETFDPSRFEGEG-PPPYTFVPFGGGPRMCPGNEFARLEILVFLHNIVKNCKWN 470

Query: 421 LCSE-EFLTRDPTLMPNKGLPIQI 443
           L +  E +  DP   P  GLPI++
Sbjct: 471 LVNPGEKVIVDPMPAPVNGLPIKL 489

BLAST of MC11g0972 vs. ExPASy Swiss-Prot
Match: A0A0U2U8U5 (Beta-amyrin 28-monooxygenase OS=Barbarea vulgaris OX=50459 GN=CYP716A81 PE=1 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.4e-86
Identity = 170/448 (37.95%), Postives = 266/448 (59.38%), Query Frame = 0

Query: 1   LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKY----GPVSKMTLFGKPTVFIHGT 60
           LPPG  G P IG+SL  L   R    E+++  RV ++      V K  LFG PT  + G 
Sbjct: 33  LPPGKTGFPLIGESLSFLSEGRQGRPEKFVTDRVRRFISSSTGVFKTHLFGYPTAVMTGA 92

Query: 61  VANKAVFTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVG 120
             NK +FT E   + +   +S+K+I      T    ++ K++R  L+ F+KP++L+KY+G
Sbjct: 93  SGNKFLFTNENKLVVSWWPDSVKKIFPYTQSTYT--DESKKLRILLMQFMKPEALRKYIG 152

Query: 121 KMDGEIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDG 180
            MD   ++H +  W + K++ V PL K LTF + C L   +++  R   +   F ++V G
Sbjct: 153 VMDEVAQRHFETLWTNQKQLIVYPLSKKLTFSVACRLFLSMDDPERVSKLEDRFNSVVTG 212

Query: 181 IWSVPINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQEEEQQDLVTCLLSMKNK 240
           I+SVPI+LP TR++ S+KAS  +++ +  ++ ++RE++ K  +   +QD+++ +L    +
Sbjct: 213 IYSVPIDLPGTRFNRSIKASRLLRKEVCAIIGQRREEL-KAGRASAEQDVLSHMLMSVGE 272

Query: 241 DKEQALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKER 300
            K     ++++ + +I +++ GHDT  I  T +I  L   P VY  VLQE +EI + K+ 
Sbjct: 273 TK-----DEDLANYLIGILIGGHDTAAIATTFIINYLAEYPHVYQRVLQEQKEILKEKKE 332

Query: 301 GEPLSWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMT 360
            E L WED+ KMKY+W VA E +RL PP+ G FR A+    F G+ IPKGW+++W+A  T
Sbjct: 333 EERLKWEDIEKMKYSWNVACEVMRLVPPLTGNFREAIDHFTFKGFYIPKGWKLYWSATAT 392

Query: 361 HLDDTIFADPHKFEPNRFDKQTLIPPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQF 420
           H++   F +P +FEPNRF+      P  +I FGGGPR+CPG EFA+LE L+ IH L+ +F
Sbjct: 393 HMNPDYFPEPERFEPNRFEGSG-PKPYSYIPFGGGPRMCPGREFARLEILVIIHNLVNRF 452

Query: 421 TWK--LCSEEFLTRDPTLMPNKGLPIQI 443
            W+    +E  +  DP   P  GLPI+I
Sbjct: 453 KWEKVFPNENKIVVDPLPKPGNGLPIRI 471

BLAST of MC11g0972 vs. NCBI nr
Match: XP_022139419.1 (taxadiene 5-alpha hydroxylase-like [Momordica charantia])

HSP 1 Score: 896 bits (2315), Expect = 0.0
Identity = 442/442 (100.00%), Postives = 442/442 (100.00%), Query Frame = 0

Query: 1   LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK 60
           LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK
Sbjct: 28  LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK 87

Query: 61  AVFTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMDG 120
           AVFTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMDG
Sbjct: 88  AVFTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMDG 147

Query: 121 EIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWSV 180
           EIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWSV
Sbjct: 148 EIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWSV 207

Query: 181 PINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQEEEQQDLVTCLLSMKNKDKEQ 240
           PINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQEEEQQDLVTCLLSMKNKDKEQ
Sbjct: 208 PINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQEEEQQDLVTCLLSMKNKDKEQ 267

Query: 241 ALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGEPL 300
           ALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGEPL
Sbjct: 268 ALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGEPL 327

Query: 301 SWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHLDD 360
           SWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHLDD
Sbjct: 328 SWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHLDD 387

Query: 361 TIFADPHKFEPNRFDKQTLIPPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQFTWKL 420
           TIFADPHKFEPNRFDKQTLIPPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQFTWKL
Sbjct: 388 TIFADPHKFEPNRFDKQTLIPPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQFTWKL 447

Query: 421 CSEEFLTRDPTLMPNKGLPIQI 442
           CSEEFLTRDPTLMPNKGLPIQI
Sbjct: 448 CSEEFLTRDPTLMPNKGLPIQI 469

BLAST of MC11g0972 vs. NCBI nr
Match: XP_038899676.1 (taxadiene 5-alpha hydroxylase-like [Benincasa hispida])

HSP 1 Score: 753 bits (1943), Expect = 2.34e-271
Identity = 365/447 (81.66%), Postives = 410/447 (91.72%), Query Frame = 0

Query: 1   LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK 60
           LPPGPLG+P+IGQSL LLRAMR+NSAEQWL +RVAKYGPVSKMTLFGKPTVF+HG  ANK
Sbjct: 30  LPPGPLGVPFIGQSLSLLRAMRTNSAEQWLQKRVAKYGPVSKMTLFGKPTVFVHGAAANK 89

Query: 61  AV-FTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMD 120
           AV F GEEG +SN+Q ESLK ILGERNLTELSGEDHKRVRGALV FL+P +LK YVGKMD
Sbjct: 90  AVVFLGEEGTVSNRQIESLKWILGERNLTELSGEDHKRVRGALVWFLRPQTLKSYVGKMD 149

Query: 121 GEIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWS 180
           GE+RKHL MYWH  KEVTV PLMK LTFDIICSLLFGIEEGP RKS++ CFKTMVDGIWS
Sbjct: 150 GEVRKHLNMYWHGKKEVTVAPLMKTLTFDIICSLLFGIEEGPTRKSIIQCFKTMVDGIWS 209

Query: 181 VPINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQEEEQ--QDLVTCLLSMKNKD 240
           VPINLPFTRY+HSLKASAK QQ+LKQLLK+K +KME+E++EE++  +DL+T LLS+KNKD
Sbjct: 210 VPINLPFTRYNHSLKASAKAQQILKQLLKDKAQKMEEEEEEEKEDDKDLITYLLSIKNKD 269

Query: 241 KEQALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERG 300
           KEQALSE+EIVHN I+LM+AGHDTT+IL+TLM+RVL +NP VYAAVLQEHEEIARSKE G
Sbjct: 270 KEQALSEEEIVHNTILLMIAGHDTTSILLTLMLRVLATNPTVYAAVLQEHEEIARSKESG 329

Query: 301 EPLSWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTH 360
           E L+WEDV KMKYTWRVA+ETLR++PPVFGGFRVAL+DI+ GGYTIPKGWQ+FWAAPMTH
Sbjct: 330 EALTWEDVSKMKYTWRVAMETLRIYPPVFGGFRVALKDIQLGGYTIPKGWQIFWAAPMTH 389

Query: 361 LDDTIFADPHKFEPNRFDK-QTLIPPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQF 420
           LD+TIF +P KFEP+RFD+ QT IPP CFIAFGGGPRICPGSEFAKLETL+TIHYLITQF
Sbjct: 390 LDETIFGEPQKFEPSRFDQNQTPIPPFCFIAFGGGPRICPGSEFAKLETLVTIHYLITQF 449

Query: 421 TWKL-CSEEFLTRDPTLMPNKGLPIQI 442
           TWKL CS++FLTRDPTLMPNKGLPIQI
Sbjct: 450 TWKLSCSQDFLTRDPTLMPNKGLPIQI 476

BLAST of MC11g0972 vs. NCBI nr
Match: XP_023535435.1 (taxadiene 5-alpha hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 741 bits (1912), Expect = 1.14e-266
Identity = 359/445 (80.67%), Postives = 408/445 (91.69%), Query Frame = 0

Query: 1   LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK 60
           LPPG LG+PYIGQSL LLRAMR+NSAEQWL +RVAKYGP+SKMTLFGKPTVF+HG  ANK
Sbjct: 29  LPPGRLGVPYIGQSLSLLRAMRANSAEQWLQQRVAKYGPISKMTLFGKPTVFVHGVAANK 88

Query: 61  AV-FTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMD 120
           AV F GEE A+SN+QTESL+RILGERNLTELSGEDHKRVRGALV FL+P SLK +VGKMD
Sbjct: 89  AVVFLGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGALVWFLRPQSLKGFVGKMD 148

Query: 121 GEIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWS 180
           GE+RKHL ++WH NKEV V PLMK LTFDIICSLLFGIEEGP RKS++ CFKTMVDGIWS
Sbjct: 149 GEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGPTRKSIIECFKTMVDGIWS 208

Query: 181 VPINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQEEEQQDLVTCLLSMKNKDKE 240
           VPINLPFTRYSHSLKASAKVQQ+LKQLLK+K +KME+E+++EE ++L+TCLLS+KNKDKE
Sbjct: 209 VPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEEEEEKEEDKNLITCLLSIKNKDKE 268

Query: 241 QALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGEP 300
           QALSE+EIVHNII+L++AGHDTT+ILITLM+RV+ +NP VYAAVLQEHE+I RSKE GE 
Sbjct: 269 QALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYAAVLQEHEDILRSKECGEA 328

Query: 301 LSWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHLD 360
           L+WEDV KMKYTWRVALETLRLFPPVFGGFR+AL+DI+ GGYTIPKGWQ+FWAA MTHLD
Sbjct: 329 LTWEDVSKMKYTWRVALETLRLFPPVFGGFRMALKDIQLGGYTIPKGWQIFWAASMTHLD 388

Query: 361 DTIFADPHKFEPNRFDK-QTLIPP-LCFIAFGGGPRICPGSEFAKLETLITIHYLITQFT 420
           + IF DP KFEP+RFD+ QT IPP L FIAFGGGPRICPG EFAKLETL+TIHY+ITQFT
Sbjct: 389 EKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYEFAKLETLVTIHYIITQFT 448

Query: 421 WKLCSEEFLTRDPTLMPNKGLPIQI 442
           WKL S++FLTRDP+LMPNKGLPIQ+
Sbjct: 449 WKLSSQDFLTRDPSLMPNKGLPIQL 473

BLAST of MC11g0972 vs. NCBI nr
Match: XP_022936436.1 (taxadiene 5-alpha hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 739 bits (1907), Expect = 7.34e-266
Identity = 361/446 (80.94%), Postives = 409/446 (91.70%), Query Frame = 0

Query: 1   LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK 60
           LPPG LG+PYIGQSL LLRAMR+NSAEQWL +RVAKYGP+SKMTLFGKPTVF+HG  ANK
Sbjct: 31  LPPGRLGVPYIGQSLSLLRAMRANSAEQWLQQRVAKYGPISKMTLFGKPTVFVHGVAANK 90

Query: 61  AV-FTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMD 120
           AV F+GEE A+SN+QTESL+RILGERNLTELSGEDHKRVRGALV FL+P SLK +VGKMD
Sbjct: 91  AVVFSGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGALVWFLRPQSLKGFVGKMD 150

Query: 121 GEIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWS 180
           GE+RKHL+++WH NKEV V PLMK LTFDIICSLLFGIEEGP RKS++ CFKTMVDGIWS
Sbjct: 151 GEVRKHLKLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGPTRKSIIECFKTMVDGIWS 210

Query: 181 VPINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQE-EEQQDLVTCLLSMKNKDK 240
           VPINLPFTRYSHSLKASAKVQQ+LKQLLK+K +KME E++E EE ++L+TCLLS+KNKDK
Sbjct: 211 VPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEGEEEEKEEDKNLITCLLSIKNKDK 270

Query: 241 EQALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGE 300
           EQALSE+EIVHNII+L++AGHDTT+ILITLM+RV+ +NP VYAAVLQEHE+I RSKE GE
Sbjct: 271 EQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYAAVLQEHEDILRSKECGE 330

Query: 301 PLSWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHL 360
            L+WEDV KMKYTWRVALETLRLFPPVFGGFRVAL+DI+ GGYTIPKGWQ+FWAA MTHL
Sbjct: 331 ALTWEDVSKMKYTWRVALETLRLFPPVFGGFRVALKDIQLGGYTIPKGWQIFWAASMTHL 390

Query: 361 DDTIFADPHKFEPNRFDK-QTLIPP-LCFIAFGGGPRICPGSEFAKLETLITIHYLITQF 420
           D+ IF DP KFEP+RFD+ QT IPP L FIAFGGGPRICPG EFAKLETL+TIHY+ITQF
Sbjct: 391 DEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYEFAKLETLVTIHYIITQF 450

Query: 421 TWKLCSEEFLTRDPTLMPNKGLPIQI 442
           TWKL S++FLTRDP+LMPNKGLPIQ+
Sbjct: 451 TWKLSSQDFLTRDPSLMPNKGLPIQL 476

BLAST of MC11g0972 vs. NCBI nr
Match: XP_022976494.1 (taxadiene 5-alpha hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 738 bits (1905), Expect = 1.43e-265
Identity = 359/446 (80.49%), Postives = 409/446 (91.70%), Query Frame = 0

Query: 1   LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK 60
           LPPGPLG+P+IGQSL LLRAMR+NSAEQWL +RVAKYGP+SKMTLFGKPTVF+HG  ANK
Sbjct: 30  LPPGPLGVPFIGQSLSLLRAMRANSAEQWLQQRVAKYGPISKMTLFGKPTVFVHGVAANK 89

Query: 61  AV-FTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMD 120
           AV F+GEE A+SN+QTESL+RILGERNLTELSGEDHKRVRGALV FL+P SLK +VGKMD
Sbjct: 90  AVVFSGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGALVWFLRPQSLKGFVGKMD 149

Query: 121 GEIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWS 180
           GE+RKHL ++WH NKEV V PLMK LTFDIICSLLFGIEEGP RKS++ CFKTMVDGIWS
Sbjct: 150 GEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGPTRKSIIECFKTMVDGIWS 209

Query: 181 VPINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQE-EEQQDLVTCLLSMKNKDK 240
           VPINLPFTRYSHSL ASAKVQQ+LKQLLK+K +KME+E++E EE ++L+TCLLS+KNKDK
Sbjct: 210 VPINLPFTRYSHSLNASAKVQQILKQLLKDKAQKMEEEEEEKEEDKNLITCLLSIKNKDK 269

Query: 241 EQALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGE 300
           EQALSE+EIVHNII+L++AGHDTT+ILITLM+RV+ +NP VYAAVLQEHE+I RSKE GE
Sbjct: 270 EQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYAAVLQEHEDIHRSKECGE 329

Query: 301 PLSWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHL 360
            L+WEDV KMKYTWRVALETLRLFPPVFGGFRVAL+DI+ GGYTIPKGWQ+FWAA MTHL
Sbjct: 330 ALTWEDVSKMKYTWRVALETLRLFPPVFGGFRVALKDIQLGGYTIPKGWQIFWAASMTHL 389

Query: 361 DDTIFADPHKFEPNRFDK-QTLIPP-LCFIAFGGGPRICPGSEFAKLETLITIHYLITQF 420
           D+ IF DP KF+P+RFD+ QT IPP L FIAFGGGPRICPG EFAKLETL+TIHY+ITQF
Sbjct: 390 DEKIFTDPQKFKPSRFDQNQTSIPPPLSFIAFGGGPRICPGYEFAKLETLVTIHYIITQF 449

Query: 421 TWKLCSEEFLTRDPTLMPNKGLPIQI 442
           TWKL S++FLTRDP+LMPNKGLPIQ+
Sbjct: 450 TWKLSSQDFLTRDPSLMPNKGLPIQL 475

BLAST of MC11g0972 vs. ExPASy TrEMBL
Match: A0A6J1CCZ3 (taxadiene 5-alpha hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111010356 PE=3 SV=1)

HSP 1 Score: 896 bits (2315), Expect = 0.0
Identity = 442/442 (100.00%), Postives = 442/442 (100.00%), Query Frame = 0

Query: 1   LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK 60
           LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK
Sbjct: 28  LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK 87

Query: 61  AVFTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMDG 120
           AVFTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMDG
Sbjct: 88  AVFTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMDG 147

Query: 121 EIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWSV 180
           EIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWSV
Sbjct: 148 EIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWSV 207

Query: 181 PINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQEEEQQDLVTCLLSMKNKDKEQ 240
           PINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQEEEQQDLVTCLLSMKNKDKEQ
Sbjct: 208 PINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQEEEQQDLVTCLLSMKNKDKEQ 267

Query: 241 ALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGEPL 300
           ALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGEPL
Sbjct: 268 ALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGEPL 327

Query: 301 SWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHLDD 360
           SWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHLDD
Sbjct: 328 SWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHLDD 387

Query: 361 TIFADPHKFEPNRFDKQTLIPPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQFTWKL 420
           TIFADPHKFEPNRFDKQTLIPPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQFTWKL
Sbjct: 388 TIFADPHKFEPNRFDKQTLIPPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQFTWKL 447

Query: 421 CSEEFLTRDPTLMPNKGLPIQI 442
           CSEEFLTRDPTLMPNKGLPIQI
Sbjct: 448 CSEEFLTRDPTLMPNKGLPIQI 469

BLAST of MC11g0972 vs. ExPASy TrEMBL
Match: A0A6J1F7G6 (taxadiene 5-alpha hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443055 PE=3 SV=1)

HSP 1 Score: 739 bits (1907), Expect = 3.55e-266
Identity = 361/446 (80.94%), Postives = 409/446 (91.70%), Query Frame = 0

Query: 1   LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK 60
           LPPG LG+PYIGQSL LLRAMR+NSAEQWL +RVAKYGP+SKMTLFGKPTVF+HG  ANK
Sbjct: 31  LPPGRLGVPYIGQSLSLLRAMRANSAEQWLQQRVAKYGPISKMTLFGKPTVFVHGVAANK 90

Query: 61  AV-FTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMD 120
           AV F+GEE A+SN+QTESL+RILGERNLTELSGEDHKRVRGALV FL+P SLK +VGKMD
Sbjct: 91  AVVFSGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGALVWFLRPQSLKGFVGKMD 150

Query: 121 GEIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWS 180
           GE+RKHL+++WH NKEV V PLMK LTFDIICSLLFGIEEGP RKS++ CFKTMVDGIWS
Sbjct: 151 GEVRKHLKLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGPTRKSIIECFKTMVDGIWS 210

Query: 181 VPINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQE-EEQQDLVTCLLSMKNKDK 240
           VPINLPFTRYSHSLKASAKVQQ+LKQLLK+K +KME E++E EE ++L+TCLLS+KNKDK
Sbjct: 211 VPINLPFTRYSHSLKASAKVQQILKQLLKDKAQKMEGEEEEKEEDKNLITCLLSIKNKDK 270

Query: 241 EQALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGE 300
           EQALSE+EIVHNII+L++AGHDTT+ILITLM+RV+ +NP VYAAVLQEHE+I RSKE GE
Sbjct: 271 EQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYAAVLQEHEDILRSKECGE 330

Query: 301 PLSWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHL 360
            L+WEDV KMKYTWRVALETLRLFPPVFGGFRVAL+DI+ GGYTIPKGWQ+FWAA MTHL
Sbjct: 331 ALTWEDVSKMKYTWRVALETLRLFPPVFGGFRVALKDIQLGGYTIPKGWQIFWAASMTHL 390

Query: 361 DDTIFADPHKFEPNRFDK-QTLIPP-LCFIAFGGGPRICPGSEFAKLETLITIHYLITQF 420
           D+ IF DP KFEP+RFD+ QT IPP L FIAFGGGPRICPG EFAKLETL+TIHY+ITQF
Sbjct: 391 DEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAFGGGPRICPGYEFAKLETLVTIHYIITQF 450

Query: 421 TWKLCSEEFLTRDPTLMPNKGLPIQI 442
           TWKL S++FLTRDP+LMPNKGLPIQ+
Sbjct: 451 TWKLSSQDFLTRDPSLMPNKGLPIQL 476

BLAST of MC11g0972 vs. ExPASy TrEMBL
Match: A0A6J1INM7 (taxadiene 5-alpha hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111476875 PE=3 SV=1)

HSP 1 Score: 738 bits (1905), Expect = 6.90e-266
Identity = 359/446 (80.49%), Postives = 409/446 (91.70%), Query Frame = 0

Query: 1   LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK 60
           LPPGPLG+P+IGQSL LLRAMR+NSAEQWL +RVAKYGP+SKMTLFGKPTVF+HG  ANK
Sbjct: 30  LPPGPLGVPFIGQSLSLLRAMRANSAEQWLQQRVAKYGPISKMTLFGKPTVFVHGVAANK 89

Query: 61  AV-FTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMD 120
           AV F+GEE A+SN+QTESL+RILGERNLTELSGEDHKRVRGALV FL+P SLK +VGKMD
Sbjct: 90  AVVFSGEEEAVSNRQTESLRRILGERNLTELSGEDHKRVRGALVWFLRPQSLKGFVGKMD 149

Query: 121 GEIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWS 180
           GE+RKHL ++WH NKEV V PLMK LTFDIICSLLFGIEEGP RKS++ CFKTMVDGIWS
Sbjct: 150 GEVRKHLNLHWHGNKEVIVAPLMKTLTFDIICSLLFGIEEGPTRKSIIECFKTMVDGIWS 209

Query: 181 VPINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQE-EEQQDLVTCLLSMKNKDK 240
           VPINLPFTRYSHSL ASAKVQQ+LKQLLK+K +KME+E++E EE ++L+TCLLS+KNKDK
Sbjct: 210 VPINLPFTRYSHSLNASAKVQQILKQLLKDKAQKMEEEEEEKEEDKNLITCLLSIKNKDK 269

Query: 241 EQALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGE 300
           EQALSE+EIVHNII+L++AGHDTT+ILITLM+RV+ +NP VYAAVLQEHE+I RSKE GE
Sbjct: 270 EQALSEEEIVHNIILLLIAGHDTTSILITLMLRVMATNPTVYAAVLQEHEDIHRSKECGE 329

Query: 301 PLSWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHL 360
            L+WEDV KMKYTWRVALETLRLFPPVFGGFRVAL+DI+ GGYTIPKGWQ+FWAA MTHL
Sbjct: 330 ALTWEDVSKMKYTWRVALETLRLFPPVFGGFRVALKDIQLGGYTIPKGWQIFWAASMTHL 389

Query: 361 DDTIFADPHKFEPNRFDK-QTLIPP-LCFIAFGGGPRICPGSEFAKLETLITIHYLITQF 420
           D+ IF DP KF+P+RFD+ QT IPP L FIAFGGGPRICPG EFAKLETL+TIHY+ITQF
Sbjct: 390 DEKIFTDPQKFKPSRFDQNQTSIPPPLSFIAFGGGPRICPGYEFAKLETLVTIHYIITQF 449

Query: 421 TWKLCSEEFLTRDPTLMPNKGLPIQI 442
           TWKL S++FLTRDP+LMPNKGLPIQ+
Sbjct: 450 TWKLSSQDFLTRDPSLMPNKGLPIQL 475

BLAST of MC11g0972 vs. ExPASy TrEMBL
Match: A0A0A0L864 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G416660 PE=3 SV=1)

HSP 1 Score: 723 bits (1865), Expect = 9.13e-260
Identity = 352/448 (78.57%), Postives = 399/448 (89.06%), Query Frame = 0

Query: 1   LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK 60
           LPPG LG+P+IGQSL LL AMR+N+AE+WL +RV KYGPVSKMTLFGKPTVFIHG  ANK
Sbjct: 30  LPPGALGVPFIGQSLSLLGAMRTNTAEKWLQKRVDKYGPVSKMTLFGKPTVFIHGAAANK 89

Query: 61  AV-FTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMD 120
           AV F+GEEG +SN+Q ESLK ILG+RNLTELSGEDHKRVRGALV FL+P +L+ YVGKMD
Sbjct: 90  AVVFSGEEGTVSNRQVESLKMILGKRNLTELSGEDHKRVRGALVWFLRPQTLRSYVGKMD 149

Query: 121 GEIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWS 180
           GE+R+HL MYWH N EVTV PLMK LTFDIICSLLFGIEEG  RKS++ CFKTMVDGIWS
Sbjct: 150 GEVRRHLNMYWHGNNEVTVAPLMKTLTFDIICSLLFGIEEGTTRKSIIECFKTMVDGIWS 209

Query: 181 VPINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQ---EEEQQDLVTCLLSMKNK 240
           +PINLPFTRY+HSLKASAK QQ+LKQLLK+K + ME+E +   EE  +DL++ LL +KNK
Sbjct: 210 IPINLPFTRYNHSLKASAKAQQILKQLLKDKAKVMEEEKESEVEENDKDLISYLLRIKNK 269

Query: 241 DKEQALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKER 300
           DKEQALSE+EIVHNII+LM+AGHDTT+IL+TLM+RVL +NP VYAAVLQEHEEI RSKER
Sbjct: 270 DKEQALSEEEIVHNIILLMIAGHDTTSILLTLMLRVLATNPTVYAAVLQEHEEIGRSKER 329

Query: 301 GEPLSWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMT 360
           GE L+WEDV KMKYTWRVA+ETLRL+PPVFGGFRVAL+DI+ G YTIPKGWQ+FWAAPMT
Sbjct: 330 GEALTWEDVSKMKYTWRVAMETLRLYPPVFGGFRVALKDIQLGAYTIPKGWQIFWAAPMT 389

Query: 361 HLDDTIFADPHKFEPNRFDK-QTLIPPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQ 420
           HLD+TIF DP KFEP+RFD+ QT IPP  FIAFGGGPRICPG EFAKLETL+TIHYLITQ
Sbjct: 390 HLDETIFGDPQKFEPSRFDQNQTPIPPFSFIAFGGGPRICPGYEFAKLETLVTIHYLITQ 449

Query: 421 FTWKL-CSEEFLTRDPTLMPNKGLPIQI 442
           FTW L CS++FLTRDPTLMPNKGLPIQI
Sbjct: 450 FTWNLSCSQDFLTRDPTLMPNKGLPIQI 477

BLAST of MC11g0972 vs. ExPASy TrEMBL
Match: A0A1S3C037 (LOW QUALITY PROTEIN: taxadiene 5-alpha hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103494945 PE=3 SV=1)

HSP 1 Score: 706 bits (1822), Expect = 2.98e-253
Identity = 344/446 (77.13%), Postives = 397/446 (89.01%), Query Frame = 0

Query: 1   LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK 60
           LPPG LG+P+IGQSL LL AMR+N+AE+WL +RV KYGPVSKMTLFGKPTVF+HG  ANK
Sbjct: 30  LPPGALGVPFIGQSLSLLGAMRTNTAEKWLQQRVDKYGPVSKMTLFGKPTVFVHGAAANK 89

Query: 61  AV-FTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMD 120
           AV F+GEEG +SN+Q ESLK ILG+RNLTELSGEDHKRVRGALV FL+P +L+ YV KMD
Sbjct: 90  AVVFSGEEGTVSNRQIESLKMILGKRNLTELSGEDHKRVRGALVWFLRPQTLRSYVEKMD 149

Query: 121 GEIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWS 180
           GE+R+HL MYWH N +VTV PLMK LTFDIICSLLFGIEEG  RKS++ CFKTMV+GIWS
Sbjct: 150 GEVRRHLNMYWHGNNKVTVAPLMKTLTFDIICSLLFGIEEGTTRKSIIQCFKTMVEGIWS 209

Query: 181 VPINLPFTRYSHSLKASAKVQQMLKQLLKEKREKME-KEDQEEEQQDLVTCLLSMKNKDK 240
           VPINLPFTRY+HSLKASAKVQQ+LKQLLK+K ++ME K+  +++ +DL+T LL +KNKDK
Sbjct: 210 VPINLPFTRYNHSLKASAKVQQILKQLLKDKAKRMEQKKKMKKKMKDLITYLLRIKNKDK 269

Query: 241 EQALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGE 300
           EQAL+E+EI+HNII+LM+AGHDTT+IL+TLM+RVL +NP VYA VLQEHEEIARSKERGE
Sbjct: 270 EQALTEEEILHNIILLMIAGHDTTSILLTLMLRVLATNPTVYADVLQEHEEIARSKERGE 329

Query: 301 PLSWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHL 360
            L+WEDV KMKYTWRVA+ETLRL+PPVFGGFRVAL+DI+ G YTIPKGWQ+FWAA  THL
Sbjct: 330 ALTWEDVSKMKYTWRVAMETLRLYPPVFGGFRVALKDIQLGAYTIPKGWQIFWAASTTHL 389

Query: 361 DDTIFADPHKFEPNRFDK-QTLIPPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQFT 420
           D+TIF DP KFEP+RFD+ QT IPP  FIAFGGGPRICPG EFAKLETL+TIHYLITQFT
Sbjct: 390 DETIFGDPQKFEPSRFDQTQTPIPPFSFIAFGGGPRICPGYEFAKLETLVTIHYLITQFT 449

Query: 421 WKL-CSEEFLTRDPTLMPNKGLPIQI 442
           W L CS++ LTRDPTLMPNKGLPIQI
Sbjct: 450 WNLSCSQDSLTRDPTLMPNKGLPIQI 475

BLAST of MC11g0972 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 319.3 bits (817), Expect = 4.8e-87
Identity = 167/448 (37.28%), Postives = 269/448 (60.04%), Query Frame = 0

Query: 1   LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGP----VSKMTLFGKPTVFIHGT 60
           LPPG  GLP IG+S   L A R    E+++  RV ++      V K  LFG PT  + G 
Sbjct: 34  LPPGNTGLPLIGESFSFLSAGRQGHPEKFITDRVRRFSSSSSCVFKTHLFGSPTAVVTGA 93

Query: 61  VANKAVFTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVG 120
             NK +FT E   + +   +S+ +I      T  S E+ +++R  L  F+KP++L++YVG
Sbjct: 94  SGNKFLFTNENKLVVSWWPDSVNKIFPSSMQTS-SKEEARKLRMLLSQFMKPEALRRYVG 153

Query: 121 KMDGEIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDG 180
            MD   ++H +  W +  +V V PL K  TF I C     +E+  R + +   F T+  G
Sbjct: 154 VMDEIAQRHFETEWANQDQVIVFPLTKKFTFSIACRSFLSMEDPARVRQLEEQFNTVAVG 213

Query: 181 IWSVPINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQEEEQQDLVTCLLSMKNK 240
           I+S+PI+LP TR++ ++KAS  +++ +  ++++++E++ K  +  E+ D+++ +L    +
Sbjct: 214 IFSIPIDLPGTRFNRAIKASRLLRKEVSAIVRQRKEEL-KAGKALEEHDILSHMLMNIGE 273

Query: 241 DKEQALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKER 300
            K++ L++      II L++ GHDT +I+ T ++  L   P VY  VLQE +EI + K+ 
Sbjct: 274 TKDEDLAD-----KIIGLLIGGHDTASIVCTFVVNYLAEFPHVYQRVLQEQKEILKEKKE 333

Query: 301 GEPLSWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMT 360
            E L WED+ KM+Y+W VA E +R+ PP+ G FR A+    F G+ IPKGW+++W+A  T
Sbjct: 334 KEGLRWEDIEKMRYSWNVACEVMRIVPPLSGTFREAIDHFSFKGFYIPKGWKLYWSATAT 393

Query: 361 HLDDTIFADPHKFEPNRFDKQTLIPPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQF 420
           H++   F +P +FEPNRF+      P  ++ FGGGPR+CPG E+A+LE LI +H L+ +F
Sbjct: 394 HMNPDYFPEPERFEPNRFEGSG-PKPYTYVPFGGGPRMCPGKEYARLEILIFMHNLVNRF 453

Query: 421 TWK--LCSEEFLTRDPTLMPNKGLPIQI 443
            W+    +E  +  DP  +P+KGLPI+I
Sbjct: 454 KWEKVFPNENKIVVDPLPIPDKGLPIRI 473

BLAST of MC11g0972 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 307.0 bits (785), Expect = 2.5e-83
Identity = 152/444 (34.23%), Postives = 266/444 (59.91%), Query Frame = 0

Query: 1   LPPGPLGLPYIGQSLGLLRAMRSNSA-EQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVAN 60
           L PG +GLP+IG+++   +A +SN   E ++  R+ K+G + K  + G PT+ ++G  AN
Sbjct: 45  LLPGEMGLPWIGETMDFYKAQKSNRVFEDFVNPRIIKHGNIFKTRIMGSPTIVVNGAEAN 104

Query: 61  KAVFTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMD 120
           + + + E   + +    S  +++G   +    GE H+ +RG + + L    L+  + K+ 
Sbjct: 105 RLILSNEFSLVVSSWPSSSVQLMGMNCIMAKQGEKHRVLRGIVANSLSYIGLESLIPKLC 164

Query: 121 GEIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWS 180
             ++ H +  W   +E+++    K+LTF ++   L+GI+       M+  F+ +++G+++
Sbjct: 165 DTVKFHHETEWRGKEEISLYRSAKVLTFTVVFECLYGIKV---EIGMLEVFERVLEGVFA 224

Query: 181 VPINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQEEEQQDLVTCLLSMKNKDKE 240
           +P+  P ++++ + KA  +++  L   ++EKR +MEKE  E+    L + L+    K   
Sbjct: 225 LPVEFPCSKFARAKKARLEIETFLVGKVREKRREMEKEGAEKPNTTLFSRLVEELIKG-- 284

Query: 241 QALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGEP 300
             ++E+E+V N+++L+ A HDTT+  +++  ++L  +P     +LQEH +I  +K  GE 
Sbjct: 285 -VITEEEVVDNMVLLVFAAHDTTSYAMSMTFKMLAQHPTCRDTLLQEHAQIKANKGEGEY 344

Query: 301 LSWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHLD 360
           L+ EDV KMKY+W+V  ET+RL PP+FG FR A+ DI++GGYTIPKGW++ W    TH +
Sbjct: 345 LTVEDVKKMKYSWQVVRETMRLSPPIFGSFRKAVADIDYGGYTIPKGWKILWTTYGTHYN 404

Query: 361 DTIFADPHKFEPNRFDKQTLIPPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQFTWK 420
             IF DP  F+P RFDK   I    ++ FGGGPR+C G + AK+  L+ +H+++T F W 
Sbjct: 405 PEIFQDPMSFDPTRFDKP--IQAYTYLPFGGGPRLCAGHQLAKISILVFMHFVVTGFDWS 464

Query: 421 LC-SEEFLTRDPTLMPNKGLPIQI 443
           L   +E ++ DP   P+ G+PI+I
Sbjct: 465 LVYPDETISMDPLPFPSLGMPIKI 480

BLAST of MC11g0972 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 242.3 bits (617), Expect = 7.5e-64
Identity = 144/443 (32.51%), Postives = 236/443 (53.27%), Query Frame = 0

Query: 1   LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK 60
           LPPG LGLP IG++  L+ A ++ + E ++  RVA+YG V    LFG+PT+F      N+
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 61  AVFTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVG-KMD 120
            V   E          S+  +LG+ +L  + G  HKR+    +SF     +K ++   +D
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 121 GEIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWS 180
             +R +L  +   +  V ++   K +TF++    L   + G   +S+   +  +++G +S
Sbjct: 151 RLVRFNLDSW---SSRVLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFS 210

Query: 181 VPINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQEEEQQDLVTCLLSMKNKDKE 240
           +P+ L  T Y  +++A  KV + L  ++ ++RE  E+E+  E ++D++  LL+      +
Sbjct: 211 LPLPLFSTTYRKAIQARRKVAEALTVVVMKRRE--EEEEGAERKKDMLAALLA-----AD 270

Query: 241 QALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGEP 300
              S++EIV  ++ L+VAG++TT+ ++TL ++ L   P   A + +EHE+I   K     
Sbjct: 271 DGFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSDSYS 330

Query: 301 LSWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHLD 360
           L W D   M +T  V  ETLR+   + G FR A+ D+E  GY IPKGW+VF +    HLD
Sbjct: 331 LEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLD 390

Query: 361 DTIFADPHKFEPNRFDKQTLI--PPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQFT 420
              F D   F P R+   ++   P   F  FGGGPR+CPG E A++   + +H L+T F+
Sbjct: 391 PNHFKDARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLCPGYELARVALSVFLHRLVTGFS 450

Query: 421 WKLCSEEFLTRDPTLMPNKGLPI 441
           W    ++ L   PT    K  PI
Sbjct: 451 WVPAEQDKLVFFPTTRTQKRYPI 463

BLAST of MC11g0972 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 238.0 bits (606), Expect = 1.4e-62
Identity = 135/443 (30.47%), Postives = 238/443 (53.72%), Query Frame = 0

Query: 2   PPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANKA 61
           P G LG P IG+++  + +  S+  E ++ +R   YG V K  +FG  T+       N+A
Sbjct: 52  PHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVNRA 111

Query: 62  VFTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMDGE 121
           V   +  A      ++++ ++G+ ++  ++G  H+R  G + SFLK   LK  + +   +
Sbjct: 112 VLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDMHK 171

Query: 122 IRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWSVP 181
                   W +++ V +  + K + F ++   L  +E+G   + +   F+  + G+ S+P
Sbjct: 172 FLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMSLP 231

Query: 182 INLPFTRYSHSLKASAKVQQMLKQLLKEK-REKMEKEDQEEEQQDLVTCLLSMKNKDKEQ 241
           IN P T+   SL+A   + + ++++++ K R+   KE+ +   +D+V  LL    KD  +
Sbjct: 232 INFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLL----KDSSE 291

Query: 242 ALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKE-RGEP 301
            L+ + I +N+I +M+ GHD+  +LITL ++ L  +PA    + +E+ ++   KE  GEP
Sbjct: 292 HLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGEP 351

Query: 302 LSWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHLD 361
           L W D   + +T +V  ETLR+   + G  R A++D+E  GY IPKGW         HLD
Sbjct: 352 LYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVHLD 411

Query: 362 DTIFADPHKFEPNRFDKQTLIPPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQFTWK 421
              +  P+KF P R+ ++ +     F  FGGG R+CPG + A+LET + +H+L+T+F W 
Sbjct: 412 KLYYESPYKFNPWRWQERDM-NTSSFSPFGGGQRLCPGLDLARLETSVFLHHLVTRFRW- 471

Query: 422 LCSEEFLTRDPTLMPNKGLPIQI 443
           +  E+ +   PT+     LPI I
Sbjct: 472 IAEEDTIINFPTVHMKNKLPIWI 488

BLAST of MC11g0972 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 230.3 bits (586), Expect = 2.9e-60
Identity = 141/443 (31.83%), Postives = 227/443 (51.24%), Query Frame = 0

Query: 1   LPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVAKYGPVSKMTLFGKPTVFIHGTVANK 60
           LPPG +G PY+G++  L     S     +   +  +YG V K  + G P V I    A K
Sbjct: 36  LPPGTMGYPYVGETFQLY----SQDPNVFFAAKQRRYGSVFKTHVLGCPCVMISSPEAAK 95

Query: 61  AVFTGEEGAMSNQQTESLKRILGERNLTELSGEDHKRVRGALVSFLKPDSLKKYVGKMDG 120
            V   +          S +R+LG++ +    G+ H ++R  ++    PD+++  V  ++ 
Sbjct: 96  FVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLRAFMPDAIRNMVPHIES 155

Query: 121 EIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLLFGIEEGPRRKSMVGCFKTMVDGIWSV 180
             ++ L  +  D  ++     MK  TF++    + G +E   R+ +  C+  +  G  S+
Sbjct: 156 IAQESLNSW--DGTQLNTYQEMKTYTFNVALISILGKDEVYYREDLKRCYYILEKGYNSM 215

Query: 181 PINLPFTRYSHSLKASAKVQQMLKQLLKEKREKMEKEDQEEEQQDLVTCLLSMKNKDKEQ 240
           PINLP T +  ++KA  ++ Q+L  +L ++R+               T LL    +DK  
Sbjct: 216 PINLPGTLFHKAMKARKELAQILANILSKRRQNPSSH----------TDLLGSFMEDK-A 275

Query: 241 ALSEDEIVHNIIILMVAGHDTTTILITLMIRVLGSNPAVYAAVLQEHEEIARSKERGEPL 300
            L++++I  NII ++ A  DTT  ++T +++ L  NP V  AV +E   I + K+ GE L
Sbjct: 276 GLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVTEEQMAIRKDKKEGESL 335

Query: 301 SWEDVGKMKYTWRVALETLRLFPPVFGGFRVALRDIEFGGYTIPKGWQVFWAAPMTHLDD 360
           +WED  KM  T+RV  ETLR    +   FR A+ D+E+ GY IPKGW+V       H + 
Sbjct: 336 TWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHNA 395

Query: 361 TIFADPHKFEPNRFDKQTLIPPLCFIAFGGGPRICPGSEFAKLETLITIHYLITQFTWKL 420
            IF+DP KF+P+RF  +    P  F+ FG G   CPG+E AKLE  + IH+L T++ W +
Sbjct: 396 DIFSDPGKFDPSRF--EVAPKPNTFMPFGSGIHSCPGNELAKLEISVLIHHLTTKYRWSI 455

Query: 421 CS-EEFLTRDPTLMPNKGLPIQI 443
               + +   P  +P  GLPI +
Sbjct: 456 VGPSDGIQYGPFALPQNGLPIAL 459