MC11g0744 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC11g0744
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionrelease factor glutamine methyltransferase
LocationMC11: 6010629 .. 6013991 (+)
RNA-Seq ExpressionMC11g0744
SyntenyMC11g0744
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGAAAAGAAGAAAAGAAGAAAAGAAAAAAGAATGACATTTTATATAAATTGAAAAGTCCATAATTTGATTTTCAGCGGTGGGAGGATAATGAGGCTAAGCATAGCTCGCTCATATCTCGTAGCCCCTCATCGCGTCATCCCGAGCCGACCCATTTTCTCTTCTTCCCTAAAACCTCAAATTCCACTGTTTCTAAGGCCACCAAATTACTCCACCACGCTCTCAGATCTCCAAAAATGGCACGATTGGGCCAAAACCCTCAATTCCTCAGTCGGATCATCCTTTTTGGAGGCAGACAACGGTCCAGATTCCACCCTTTTGAACCGAGAGCTCAAATGGCTGATTGAAGATGCAGTTGAAGAAGAATCAAAATCCCTAAGTTCCGAACTGGGAATTGACGATGTCAAGCGAAATCGTGAGCAGGGCCTGAGAAATGTGAGGTTGAAGGTGGGAATCGAGGAGCTCTATAGGCTGTGGAAGCAGAGGATCCATGAGAGGCGGCCGTTTCAGTACATTGTTGGGTGCGAGCATTGGAGGGATTTGGTGTTGAGTGTGGAAGAAGGAGTGCTGATTCCGAGGCCTGAGACGGAGCTTTTGGTGGATTTGGTGGCGAAAGTGATTTCTGATAAGGAAGCACTTGGAGGAGAAGGGTTTTGGGTTGATTTGGGTACTGGAAGTGGTGCAATTGCTATTGGTATTGGCAGGATTTTGGATGGTCGTGCTAGAGTTATTGCCACTGATGTAAGCCCCACGGCACTGGCAGTCGCCGCCTATAACGTTCAGAGGTATGGTTTACAGGTTAGTTTAAACTAAGCATGAACATGAATACTTATGGATAAGCCTTATAGCCTACTTTTCATAGTGACATTTTCTTTACTAATGAATGCTTTTGTTGATGATTGAGGTGCGTTTGATAATCGATTTGTTTTTTATTTTCTGTTTTTTGTGTTTGTCGTCTACTTTATAAACACAAAACGAAAATGTAAGTTAAAATTTTGGGCAAGCCCTTCCGGGCCGGTGATCTCTCTGTCATATTGGTTTAGAGGTATCGAGTTCGAACCTTTTAATCAGCTTAATACCAAAAATCCTTGACATCTCTCGGATCCGAGCCTTGGGGCAGGCGTGGGTGCCCTTGGGTATAGGGAAGCAAAGCTCCGACTCCTAGTTATCTTAAAAAAATTAAAGACAAAATCGTTATCAAACAAGATTTAAGAAGGGTTCTCAGAAAGGACAACGATAATTTGAATTGTTTCGTTTATGGTAGCCGTTTATAATCTCTAGTTTCAATGTTGAATCAGGAGTTGATTGAGTTAAGGCAAGGATCATGGTACGAGCCGTTAAAGGATGTCCAAGGAAGGGTATCTGGAATTATAAGTAATCCTCCCTACATACCCAGTGATAATATTTTTGGCCTTCAAGCTGAGGTTTGTAAACATGAACCTAGAGTTGCATTGGATGGAGGCACTAATGGCATGGATGAGCTTATCCATCTATGTGATGAAGCTGCTTTAATGTTGAAACCTGGTGGCTTCTTTGCTTTTGAGGTACCCTTGTTTCCTAATTTCTACAAGTTTGTTACATTTAAAAAAAAACATTGAAGTTTATTGTTTCGGGTTAAAAAACAAGTTTTCAGTTCTTTTGAATGTCTTTTATCATGGGGTAATTAGGAATATTTGTAGGTTAATAAGGGCATATTAGTTATTAACCAATTGGTTATAAATAGAGGAAGTGAGATGAGATGAAGGTAGACATTGATTTAGTTGGTCGAGACTTGAATGATCTCAGGACGAGTCTAAGTACCTCAAATTACTTCTTTCTGTATTTTTTTTCATCATCGATATATTTTTTGAGTCTCTAAGATCGATACCAGACTTCAAAGAAACACCCTCGACCTTTTGAAATCTTTGAAATCTATTTATAACATTGTCATTTCATCTTCTTGCAGACAAATGGTGAAGATCAGTGCAAGAATCTAGTGAAGTACATGGAAAGTATTCACAGAGGGAGGTTTTGCCATCTGGAAATGGTGTCTGATTTTGCAGCCATTCCTAGATTTGTAACTGGATTCCTCAAAGAATAGTGTCTTTCTTGATCTCAGCTGAAGCTGCCTACTTAAAAAAAACCAACAAAATGAGTTCAGATCAATTCACATTCAGCTCTTTAGTCTAGATTAGATAGCTGTTTTTAATTTCATCTTTTCTAAGCTTCAAAATTATTATAAGCTCCAAATACAAATTAGAAGCAAATCATTTGTTGAGCATTTTAGGAACACTCCTGCATTGGGATAGCAGAAGAAGCCCAAGTTTGATGTGATTCAATTTTGTGGGATTTCAATTACTGGTAATCACTTATCTTATCTTACTATATTACTATTTTACTATTGACCACCGTTGCAAACGAGTTATCATTTTTTTAAAAAGAGCAAAAAAATGCTTCTCTTCCCATGTTTCTCTCTTATTATGTTAAAATTTGTTGGTCAACAAGAGATGGGATCGGAACAGGGCTAAGCGATCTGTCGAAGCTGTGGGACGGAAAAATGCATCGGCAAGGGAGCGTTCCGCCTTAGAGGGAAGCACCCGTGCGAGCGGTGGTGGACGAAGCGGAAACGAGAATGTCGGCTTGAGTAATGCAAACATTGGTGAGAATCCAATGCCCTGAAAACCTAAGGGTTCTTCCACAAGGTTCGTCCACAGAGGGTGAGTCAGGGCCTAAGATCAGGTCGAAAGGTGTAGTCGATAGACAACAGGTGAATATTCATGTACTATCCCTTGTTGATCCTGAGGGAGGGAGGAGGCTAGGTTAGTCGAAAGATGGTTATTGGTTCAAGGATGCAAGGTGCCTCTGCTTTTTTAGGGTAAGAAGGGGTAGAGAAAATACCTCGAGTTAATGTTCGAGGACCAGGCGCTACGACGCTGAAGTAACCCATGCCATACTCCCAAAAAAAGCTCGAATGACCTTCAACAAAAGGGTACCTGTACCCGAAACCGACACAGGTGGGTAGGTAGAGAATACTTAGGGACGCGAGACAACTCTTTCTAAGGAACTAACACCTAAATATTGTTTGAGTTTCAATTCTTCTAATGCTCTTTATGATCTAACCTGATCAATCATAATATTTCACTCGTGATCTGGTCAGGTCCAATCATGATATTAATGGATAAGGTATTTTCTCGAAAAACACCGGATCTCGAGAAGAGTTGAAATGATGCAGGATCAAATCCTTACATTATTCAATGGAAGCTTAGCTCGCCTCACTTGCTTTCTTTATTGTATAAGGGCACAAACGAAGTCAATAGATTTGCTAGTTGAATAGATTTCCTTCTAACTAGCAGGAGCTACTCCAATCCCATTTTTTGTTCATTC

mRNA sequence

AAAGAAAAGAAGAAAAGAAGAAAAGAAAAAAGAATGACATTTTATATAAATTGAAAAGTCCATAATTTGATTTTCAGCGGTGGGAGGATAATGAGGCTAAGCATAGCTCGCTCATATCTCGTAGCCCCTCATCGCGTCATCCCGAGCCGACCCATTTTCTCTTCTTCCCTAAAACCTCAAATTCCACTGTTTCTAAGGCCACCAAATTACTCCACCACGCTCTCAGATCTCCAAAAATGGCACGATTGGGCCAAAACCCTCAATTCCTCAGTCGGATCATCCTTTTTGGAGGCAGACAACGGTCCAGATTCCACCCTTTTGAACCGAGAGCTCAAATGGCTGATTGAAGATGCAGTTGAAGAAGAATCAAAATCCCTAAGTTCCGAACTGGGAATTGACGATGTCAAGCGAAATCGTGAGCAGGGCCTGAGAAATGTGAGGTTGAAGGTGGGAATCGAGGAGCTCTATAGGCTGTGGAAGCAGAGGATCCATGAGAGGCGGCCGTTTCAGTACATTGTTGGGTGCGAGCATTGGAGGGATTTGGTGTTGAGTGTGGAAGAAGGAGTGCTGATTCCGAGGCCTGAGACGGAGCTTTTGGTGGATTTGGTGGCGAAAGTGATTTCTGATAAGGAAGCACTTGGAGGAGAAGGGTTTTGGGTTGATTTGGGTACTGGAAGTGGTGCAATTGCTATTGGTATTGGCAGGATTTTGGATGGTCGTGCTAGAGTTATTGCCACTGATGTAAGCCCCACGGCACTGGCAGTCGCCGCCTATAACGTTCAGAGGTATGGTTTACAGGAGTTGATTGAGTTAAGGCAAGGATCATGGTACGAGCCGTTAAAGGATGTCCAAGGAAGGGTATCTGGAATTATAAGTAATCCTCCCTACATACCCAGTGATAATATTTTTGGCCTTCAAGCTGAGGTTTGTAAACATGAACCTAGAGTTGCATTGGATGGAGGCACTAATGGCATGGATGAGCTTATCCATCTATGTGATGAAGCTGCTTTAATGTTGAAACCTGGTGGCTTCTTTGCTTTTGAGACAAATGGTGAAGATCAGTGCAAGAATCTAGTGAAGTACATGGAAAGTATTCACAGAGGGAGGTTTTGCCATCTGGAAATGGTGTCTGATTTTGCAGCCATTCCTAGATTTGTAACTGGATTCCTCAAAGAATAGTGTCTTTCTTGATCTCAGCTGAAGCTGCCTACTTAAAAAAAACCAACAAAATGAGTTCAGATCAATTCACATTCAGCTCTTTAGTCTAGATTAGATAGCTGTTTTTAATTTCATCTTTTCTAAGCTTCAAAATTATTATAAGCTCCAAATACAAATTAGAAGCAAATCATTTGTTGAGCATTTTAGGAACACTCCTGCATTGGGATAGCAGAAGAAGCCCAAGTTTGATGTGATTCAATTTTGTGGGATTTCAATTACTGAGATGGGATCGGAACAGGGCTAAGCGATCTGTCGAAGCTGTGGGACGGAAAAATGCATCGGCAAGGGAGCGTTCCGCCTTAGAGGGAAGCACCCGTGCGAGCGGTGGTGGACGAAGCGGAAACGAGAATGTCGGCTTGAGTAATGCAAACATTGGTGAGAATCCAATGCCCTGAAAACCTAAGGGTTCTTCCACAAGGTTCGTCCACAGAGGGTGAGTCAGGGCCTAAGATCAGGTCGAAAGGTGTAGTCGATAGACAACAGGTGAATATTCATGTACTATCCCTTGTTGATCCTGAGGGAGGGAGGAGGCTAGGTTAGTCGAAAGATGGTTATTGGTTCAAGGATGCAAGGTGCCTCTGCTTTTTTAGGGTAAGAAGGGGTAGAGAAAATACCTCGAGTTAATGTTCGAGGACCAGGCGCTACGACGCTGAAGTAACCCATGCCATACTCCCAAAAAAAGCTCGAATGACCTTCAACAAAAGGGTACCTGTACCCGAAACCGACACAGGTGGGTAGGTAGAGAATACTTAGGGACGCGAGACAACTCTTTCTAAGGAACTAACACCTAAATATTGTTTGAGTTTCAATTCTTCTAATGCTCTTTATGATCTAACCTGATCAATCATAATATTTCACTCGTGATCTGGTCAGGTCCAATCATGATATTAATGGATAAGGTATTTTCTCGAAAAACACCGGATCTCGAGAAGAGTTGAAATGATGCAGGATCAAATCCTTACATTATTCAATGGAAGCTTAGCTCGCCTCACTTGCTTTCTTTATTGTATAAGGGCACAAACGAAGTCAATAGATTTGCTAGTTGAATAGATTTCCTTCTAACTAGCAGGAGCTACTCCAATCCCATTTTTTGTTCATTC

Coding sequence (CDS)

ATGAGGCTAAGCATAGCTCGCTCATATCTCGTAGCCCCTCATCGCGTCATCCCGAGCCGACCCATTTTCTCTTCTTCCCTAAAACCTCAAATTCCACTGTTTCTAAGGCCACCAAATTACTCCACCACGCTCTCAGATCTCCAAAAATGGCACGATTGGGCCAAAACCCTCAATTCCTCAGTCGGATCATCCTTTTTGGAGGCAGACAACGGTCCAGATTCCACCCTTTTGAACCGAGAGCTCAAATGGCTGATTGAAGATGCAGTTGAAGAAGAATCAAAATCCCTAAGTTCCGAACTGGGAATTGACGATGTCAAGCGAAATCGTGAGCAGGGCCTGAGAAATGTGAGGTTGAAGGTGGGAATCGAGGAGCTCTATAGGCTGTGGAAGCAGAGGATCCATGAGAGGCGGCCGTTTCAGTACATTGTTGGGTGCGAGCATTGGAGGGATTTGGTGTTGAGTGTGGAAGAAGGAGTGCTGATTCCGAGGCCTGAGACGGAGCTTTTGGTGGATTTGGTGGCGAAAGTGATTTCTGATAAGGAAGCACTTGGAGGAGAAGGGTTTTGGGTTGATTTGGGTACTGGAAGTGGTGCAATTGCTATTGGTATTGGCAGGATTTTGGATGGTCGTGCTAGAGTTATTGCCACTGATGTAAGCCCCACGGCACTGGCAGTCGCCGCCTATAACGTTCAGAGGTATGGTTTACAGGAGTTGATTGAGTTAAGGCAAGGATCATGGTACGAGCCGTTAAAGGATGTCCAAGGAAGGGTATCTGGAATTATAAGTAATCCTCCCTACATACCCAGTGATAATATTTTTGGCCTTCAAGCTGAGGTTTGTAAACATGAACCTAGAGTTGCATTGGATGGAGGCACTAATGGCATGGATGAGCTTATCCATCTATGTGATGAAGCTGCTTTAATGTTGAAACCTGGTGGCTTCTTTGCTTTTGAGACAAATGGTGAAGATCAGTGCAAGAATCTAGTGAAGTACATGGAAAGTATTCACAGAGGGAGGTTTTGCCATCTGGAAATGGTGTCTGATTTTGCAGCCATTCCTAGATTTGTAACTGGATTCCTCAAAGAATAG

Protein sequence

MRLSIARSYLVAPHRVIPSRPIFSSSLKPQIPLFLRPPNYSTTLSDLQKWHDWAKTLNSSVGSSFLEADNGPDSTLLNRELKWLIEDAVEEESKSLSSELGIDDVKRNREQGLRNVRLKVGIEELYRLWKQRIHERRPFQYIVGCEHWRDLVLSVEEGVLIPRPETELLVDLVAKVISDKEALGGEGFWVDLGTGSGAIAIGIGRILDGRARVIATDVSPTALAVAAYNVQRYGLQELIELRQGSWYEPLKDVQGRVSGIISNPPYIPSDNIFGLQAEVCKHEPRVALDGGTNGMDELIHLCDEAALMLKPGGFFAFETNGEDQCKNLVKYMESIHRGRFCHLEMVSDFAAIPRFVTGFLKE
Homology
BLAST of MC11g0744 vs. ExPASy Swiss-Prot
Match: P74003 (Release factor glutamine methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=prmC PE=3 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.5e-46
Identity = 106/278 (38.13%), Postives = 158/278 (56.83%), Query Frame = 0

Query: 80  ELKWLIEDAVEEESKSLSSELGIDDVKRNREQGLRNVRLKVGIEELYRLWKQRIHERRPF 139
           EL WL++   + +  +L          R ++   R + L+   E + R W++R+ E+ P 
Sbjct: 31  ELNWLLQGWTDLDRLTL----------RLQDFAHREIALQETWENIQRGWRRRVEEKYPV 90

Query: 140 QYIVGCEHWRDLVLSVEEGVLIPRPETELLVDLVAKVISDKEALGGEGFWVDLGTGSGAI 199
           QY++G   WRD V+ V + VLIPRPETEL++D+V    S          WVDLGTGSGAI
Sbjct: 91  QYLLGQTQWRDFVIKVTDDVLIPRPETELIIDIVQHEHSALSPSNCADHWVDLGTGSGAI 150

Query: 200 AIGIGRILDGRARVIATDVSPTALAVAAYNVQRYGLQELIELRQGSWYEPLKDVQGRVSG 259
           A+G+      +A V A D S +ALA+A  N Q     + I+  QG W+EPL+ ++G+V G
Sbjct: 151 ALGLAATFP-QALVHAVDCSGSALAIARENAQLNQFGDRIQFHQGYWWEPLEHLKGQVQG 210

Query: 260 IISNPPYIPSDNIFGLQAEVCKHEPRVALDGGTNGMDELIHLCDEAALMLKPGGFFAFET 319
           ++SNPPYIP   +  LQ EV KHEP +ALDGG +G+  +  L   +   LKPGGF+  E 
Sbjct: 211 MVSNPPYIPQRELAQLQPEVIKHEPLLALDGGPDGLQAVEQLIRRSPTYLKPGGFWLVEI 270

Query: 320 NGEDQCKNLVKYMESIHRGRFCHLEMVSDFAAIPRFVT 358
               Q   + + + +   G +  +++  D A+I RFV+
Sbjct: 271 M-TGQAPMVAELLRA--SGAYQDIQIHRDLASIERFVS 294

BLAST of MC11g0744 vs. ExPASy Swiss-Prot
Match: Q8DHV7 (Release factor glutamine methyltransferase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=prmC PE=3 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 9.0e-44
Identity = 95/233 (40.77%), Postives = 139/233 (59.66%), Query Frame = 0

Query: 86  EDAVEEESKSLSSELGIDDVKRNREQGLRNVRLKVGIEELYRLWKQRIHERRPFQYIVGC 145
           E  + E  + L +  G+  ++    +    + LK+ + EL   W++R  ER P QY++G 
Sbjct: 23  ESGLRELKQFLRAFTGLSPLEITLRRFPPQIHLKLPLTELQERWQRRWQERVPLQYLIGV 82

Query: 146 EHWRDLVLSVEEGVLIPRPETELLVDLVAKVISDKEALGGEGFWVDLGTGSGAIAIGIGR 205
            HW DL L V   VLIPRPETE L+ +VA  +   +    +G W+DLGTGSGAIAIG+ R
Sbjct: 83  AHWHDLELVVTPSVLIPRPETEELLAVVAATVPPWQQ---QGHWLDLGTGSGAIAIGLAR 142

Query: 206 ILDGRARVIATDVSPTALAVAAYNVQRYGLQELIELRQGSWYEPLKDVQGRVSGIISNPP 265
           +    A + A D S  AL VA  N+Q+Y L + +    G+W++P+  +QG+V GI+SNPP
Sbjct: 143 LFPA-ALIHAVDCSSEALEVAQVNIQKYALGDRVRCYVGNWFDPIVPLQGQVQGIVSNPP 202

Query: 266 YIPSDNIFGLQAEVCKHEPRVALDGGTNGMDELIHLCDEAALMLKPGGFFAFE 319
           YIP+  +  LQ EV  HEP +ALDGGT+G+  +  + + A   L+P G+   E
Sbjct: 203 YIPTSVVATLQPEVQYHEPLLALDGGTDGLQAIRQILETAPEYLQPQGWLFIE 251

BLAST of MC11g0744 vs. ExPASy Swiss-Prot
Match: Q7NJS7 (Release factor glutamine methyltransferase OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=prmC PE=3 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 3.4e-43
Identity = 107/256 (41.80%), Postives = 143/256 (55.86%), Query Frame = 0

Query: 112 GLRNVRLKVG--------IEELYRLWKQRIHERRPFQYIVGCEHWRDLVLSVEEGVLIPR 171
           GL  +R+++G         E+L  LW++RI E  P QY++G  HWRDL L V   VLIPR
Sbjct: 35  GLDRLRVRLGGPQALEAHREKLAALWRRRIEEAMPLQYLLGTAHWRDLQLQVNPAVLIPR 94

Query: 172 PETELLVDLVAKVISDKEALGGEGFWVDLGTGSGAIAIGIGRILDGRARVIATDVSPTAL 231
           PE+E LVD+       +   G     VDLGTGSGAIA+ + R L G A V A D S  AL
Sbjct: 95  PESEALVDVAVDFC--RSCAGAR--VVDLGTGSGAIAVAVARALPG-ATVWAVDASEAAL 154

Query: 232 AVAAYNVQRYGLQELIELRQGSWYEPLKDVQGRVSGIISNPPYIPSDNIFGLQAEVCKHE 291
            VA  N++RYGL E + L +G+W+ PL         ++SNPPYIPS  I  L  EV  HE
Sbjct: 155 VVAGANIERYGLSEQVHLLRGNWFVPLPTQP--FDAVLSNPPYIPSAEIAALMPEVRLHE 214

Query: 292 PRVALDGGTNGMDELIHLCDEAALMLKPGGFFAFETNGEDQCKNLVKYMESIHRGRFCHL 351
           P  ALDGG++G+D +  +  +AA  L+PGG  A E     Q   +V+ +      R+  +
Sbjct: 215 PLSALDGGSDGLDAVRQIIADAARHLRPGGILALEVMA-GQGPTVVQLL--ARDSRYGCI 274

Query: 352 EMVSDFAAIPRFVTGF 360
             V D+A I R V  +
Sbjct: 275 RTVRDWAGIERIVVTY 280

BLAST of MC11g0744 vs. ExPASy Swiss-Prot
Match: Q7VDL7 (Release factor glutamine methyltransferase OS=Prochlorococcus marinus (strain SARG / CCMP1375 / SS120) OX=167539 GN=prmC PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 9.3e-33
Identity = 85/242 (35.12%), Postives = 123/242 (50.83%), Query Frame = 0

Query: 117 RLKVGIEELYRLWKQRIHERRPFQYIVGCEHWRDLVLSVEEGVLIPRPETELLVDLVAKV 176
           +L + +EEL  +W + + ++ P Q++VG   WRD  L V    LIPR ETE+L+D+  K 
Sbjct: 56  KLDLSLEELSLIWMRHLSDQIPLQHLVGKCPWRDFELKVNSSALIPRQETEILIDIALKK 115

Query: 177 ISDKEALGGEGFWVDLGTGSGAIAIGIGRIL---DGRARVIATDVSPTALAVAAYNVQRY 236
           +     L   G W DLGTGSGA+A+ + R L   +G     A D    ALA+A  N+   
Sbjct: 116 VD--AGLMKYGRWADLGTGSGALAVALARALPLWEGH----AADCCNDALALAESNINTL 175

Query: 237 GLQELIELRQGSWYEPLKDVQGRVSGIISNPPYIPSDNIFGLQAEVCKHEPRVALDGGTN 296
                + L  G W+EPLK   G    +++NPPYIP  ++  L   V  HEP +AL GG +
Sbjct: 176 TENANVSLHLGDWWEPLKPWWGNFDLVVANPPYIPKTHLSELDPVVRDHEPILALSGGDD 235

Query: 297 GMDELIHLCDEAALMLKPGGFFAFETNGEDQCKNLVKYMESIHRGRFCHLEMVSDFAAIP 356
           GMD    +   A   L+ GG+   E N  DQ +  +  M       F  ++  +D   + 
Sbjct: 236 GMDSCRKVIKGAMKGLRSGGWLLLEHN-FDQSEQALNLMVD---SGFLEVDFENDLEGVR 287

BLAST of MC11g0744 vs. ExPASy Swiss-Prot
Match: Q2RFW1 (Release factor glutamine methyltransferase OS=Moorella thermoacetica (strain ATCC 39073 / JCM 9320) OX=264732 GN=prmC PE=3 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.3e-31
Identity = 89/239 (37.24%), Postives = 134/239 (56.07%), Query Frame = 0

Query: 127 RLWKQRIHERR---PFQYIVGCEHWRDLVLSVEEGVLIPRPETELLVDLVAKVISDKEAL 186
           R W Q I  R    P QY+ G + +  L   V   VLIPR +TE++V+ V + +   E+ 
Sbjct: 56  RFW-QAIDRRAAGYPLQYLTGHQEFMSLDFKVTPAVLIPRQDTEVVVEAVLERLDPCESY 115

Query: 187 GGEGFWVDLGTGSGAIAIGIGRILDGRARVIATDVSPTALAVAAYNVQRYGLQELIELRQ 246
                  D GTGSGAIA+ +   L  RARV ATD+SP AL VA  N ++ GL   + L Q
Sbjct: 116 ----TIADCGTGSGAIALSLAHYLP-RARVYATDISPAALTVAQENARKLGLAARVTLLQ 175

Query: 247 GSWYEPLKDVQGRVSGIISNPPYIPSDNIFGLQAEVCKHEPRVALDGGTNGMDELIHLCD 306
           G +  PL+ +  ++  +++NPPYIP+  + GL A+V + EPR+ALDGG +G+D    L  
Sbjct: 176 GDFLAPLRGL--KLDALVANPPYIPTAALPGLPADV-RSEPRLALDGGPDGLDAYRFLLP 235

Query: 307 EAALMLKPGGFFAFETNGEDQCKNLVKYMESIHRGRFCHLEMVSDFAAIPRFVTGFLKE 363
            AA +L+PGG  A E  G DQ + +     ++  G + + +++ D+A   R    + +E
Sbjct: 236 GAAGLLRPGGLLALEI-GSDQGQAVKDLARAV--GAYRNEQVLPDYAGRDRCFLAYRRE 282

BLAST of MC11g0744 vs. NCBI nr
Match: XP_022141020.1 (uncharacterized protein LOC111011525 [Momordica charantia])

HSP 1 Score: 708 bits (1828), Expect = 3.28e-257
Identity = 355/362 (98.07%), Postives = 357/362 (98.62%), Query Frame = 0

Query: 1   MRLSIARSYLVAPHRVIPSRPIFSSSLKPQIPLFLRPPNYSTTLSDLQKWHDWAKTLNSS 60
           MRLSIARSYLVAPHRVIPSRPIFSSSLKPQIPLFLRPPNYSTTLSD+QKWHDWAKTLNSS
Sbjct: 1   MRLSIARSYLVAPHRVIPSRPIFSSSLKPQIPLFLRPPNYSTTLSDVQKWHDWAKTLNSS 60

Query: 61  VGSSFLEADNGPDSTLLNRELKWLIEDAVEEESKSLSSELGIDDVKRNREQGLRNVRLKV 120
           VGSSFLEADNGPDSTLLNRELKWLIEDAVEEESKSLSSELGIDDVKRNREQGLRNVRLKV
Sbjct: 61  VGSSFLEADNGPDSTLLNRELKWLIEDAVEEESKSLSSELGIDDVKRNREQGLRNVRLKV 120

Query: 121 GIEELYRLWKQRIHERRPFQYIVGCEHWRDLVLSVEEGVLIPRPETELLVDLVAKVISDK 180
           GIEELYRLWKQRIHERRPFQYIVGCEHWRDLVLSVEEGVLIPRPETELLVDL     +DK
Sbjct: 121 GIEELYRLWKQRIHERRPFQYIVGCEHWRDLVLSVEEGVLIPRPETELLVDL-----ADK 180

Query: 181 EALGGEGFWVDLGTGSGAIAIGIGRILDGRARVIATDVSPTALAVAAYNVQRYGLQELIE 240
           EALGGEGFWVDLGTGSGAIAIGIGRILDGRARVIATDVSPTALAVAAYNVQRYGLQELIE
Sbjct: 181 EALGGEGFWVDLGTGSGAIAIGIGRILDGRARVIATDVSPTALAVAAYNVQRYGLQELIE 240

Query: 241 LRQGSWYEPLKDVQGRVSGIISNPPYIPSDNIFGLQAEVCKHEPRVALDGGTNGMDELIH 300
           LRQGSWYEPLKDVQGRVSGIISNPPYIPSDNIFGLQAEVCKHEPRVALDGGTNGMDELIH
Sbjct: 241 LRQGSWYEPLKDVQGRVSGIISNPPYIPSDNIFGLQAEVCKHEPRVALDGGTNGMDELIH 300

Query: 301 LCDEAALMLKPGGFFAFETNGEDQCKNLVKYMESIHRGRFCHLEMVSDFAAIPRFVTGFL 360
           LCDEAALMLKPGGFFAFETNGEDQCKNLVKYMESIHRGRFCHLEMVSDFAAIPRFVTGFL
Sbjct: 301 LCDEAALMLKPGGFFAFETNGEDQCKNLVKYMESIHRGRFCHLEMVSDFAAIPRFVTGFL 357

Query: 361 KE 362
           KE
Sbjct: 361 KE 357

BLAST of MC11g0744 vs. NCBI nr
Match: XP_022936763.1 (uncharacterized protein LOC111443257 [Cucurbita moschata] >XP_022936764.1 uncharacterized protein LOC111443257 [Cucurbita moschata])

HSP 1 Score: 590 bits (1520), Expect = 5.40e-210
Identity = 296/362 (81.77%), Postives = 327/362 (90.33%), Query Frame = 0

Query: 1   MRLSIARSYLVAPHRVIPSRPIFSSSLKPQIPLFLRPPNYSTTLSDLQKWHDWAKTLNSS 60
           MRLSI+R+YL AP RV PSR I S+S KP+IPLFLRPPNYS TL+DLQKWHDWAKTL+SS
Sbjct: 16  MRLSISRAYLAAPRRVRPSRSICSASSKPRIPLFLRPPNYSATLTDLQKWHDWAKTLSSS 75

Query: 61  VGSSFLEADNGPDSTLLNRELKWLIEDAVEEESKSLSSELGIDDVKRNREQGLRNVRLKV 120
           VGSSF++ADNGPDS LL+RELKWLIEDAVE+  KS+SSEL  +  ++N E   RNVRLKV
Sbjct: 76  VGSSFVDADNGPDSALLHRELKWLIEDAVED--KSVSSELRTNS-EQNHEHNHRNVRLKV 135

Query: 121 GIEELYRLWKQRIHERRPFQYIVGCEHWRDLVLSVEEGVLIPRPETELLVDLVAKVISDK 180
           GIEELY +WKQRIHERRPFQYIVGCEHWRDL+LSVEEGVLIPRPETE+LVDLVA+V+SD 
Sbjct: 136 GIEELYGVWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVAEVVSDN 195

Query: 181 EALGGEGFWVDLGTGSGAIAIGIGRILDGRARVIATDVSPTALAVAAYNVQRYGLQELIE 240
           EAL  EG WVDLGTGSGAIAI I R+L+GRARVIATD+SPTAL VA YNVQRYGLQ+ IE
Sbjct: 196 EALR-EGLWVDLGTGSGAIAIAICRVLEGRARVIATDLSPTALTVAGYNVQRYGLQDSIE 255

Query: 241 LRQGSWYEPLKDVQGRVSGIISNPPYIPSDNIFGLQAEVCKHEPRVALDGGTNGMDELIH 300
           LRQGSWYEPLKDVQG++SGIISNPPYIPSDNI GLQAEV KHEPRVALDGGTNGMDELIH
Sbjct: 256 LRQGSWYEPLKDVQGKLSGIISNPPYIPSDNIVGLQAEVGKHEPRVALDGGTNGMDELIH 315

Query: 301 LCDEAALMLKPGGFFAFETNGEDQCKNLVKYMESIHRGRFCHLEMVSDFAAIPRFVTGFL 360
           LCDEAA+MLKPGGFFAFETNGEDQCK+LVKYME  HRGR C+L++VSDFA IPRF+TGFL
Sbjct: 316 LCDEAAVMLKPGGFFAFETNGEDQCKHLVKYMEDNHRGRLCNLKIVSDFAGIPRFITGFL 373

Query: 361 KE 362
            E
Sbjct: 376 NE 373

BLAST of MC11g0744 vs. NCBI nr
Match: XP_023534998.1 (uncharacterized protein LOC111796557 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 589 bits (1519), Expect = 6.87e-210
Identity = 297/362 (82.04%), Postives = 324/362 (89.50%), Query Frame = 0

Query: 1   MRLSIARSYLVAPHRVIPSRPIFSSSLKPQIPLFLRPPNYSTTLSDLQKWHDWAKTLNSS 60
           MRLSI+R+YL APHRV PSR I SSS KP+IPLFLRPPNYS TL+DLQKWHDWAKTL+SS
Sbjct: 13  MRLSISRAYLAAPHRVSPSRSICSSSSKPRIPLFLRPPNYSATLTDLQKWHDWAKTLSSS 72

Query: 61  VGSSFLEADNGPDSTLLNRELKWLIEDAVEEESKSLSSELGIDDVKRNREQGLRNVRLKV 120
           VGSSF++ADNGPDS LL+RELKWLI+DAVE+  KS+SSEL  +  ++N E  LRNVRLKV
Sbjct: 73  VGSSFVDADNGPDSALLHRELKWLIDDAVED--KSVSSELRTNS-EQNHEHNLRNVRLKV 132

Query: 121 GIEELYRLWKQRIHERRPFQYIVGCEHWRDLVLSVEEGVLIPRPETELLVDLVAKVISDK 180
           GIEELY LWKQRIHERRPFQYIVGCEHWRDL+LSVEEGVLIPRPETE+LVDLVA+V+SD 
Sbjct: 133 GIEELYGLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVAEVVSDN 192

Query: 181 EALGGEGFWVDLGTGSGAIAIGIGRILDGRARVIATDVSPTALAVAAYNVQRYGLQELIE 240
           EAL  EG WVDLGTGSGAIAI I R+L+GR RVIATD+SPTAL VA YNVQRYGLQE IE
Sbjct: 193 EALR-EGLWVDLGTGSGAIAIAICRVLEGRGRVIATDLSPTALTVAGYNVQRYGLQESIE 252

Query: 241 LRQGSWYEPLKDVQGRVSGIISNPPYIPSDNIFGLQAEVCKHEPRVALDGGTNGMDELIH 300
           LRQGSWYEPLKDV+G +SGIISNPPYIPS NI GLQAEV KHEPRVALDGGTNGMDELIH
Sbjct: 253 LRQGSWYEPLKDVRGMLSGIISNPPYIPSGNIVGLQAEVGKHEPRVALDGGTNGMDELIH 312

Query: 301 LCDEAALMLKPGGFFAFETNGEDQCKNLVKYMESIHRGRFCHLEMVSDFAAIPRFVTGFL 360
           LCDEAA+MLKPGGFFAFETNGEDQCK+LVKYME  HRGR C L++VSDFA IPRF TGFL
Sbjct: 313 LCDEAAVMLKPGGFFAFETNGEDQCKHLVKYMEDNHRGRLCDLKIVSDFAGIPRFTTGFL 370

Query: 361 KE 362
            E
Sbjct: 373 NE 370

BLAST of MC11g0744 vs. NCBI nr
Match: XP_004151022.1 (uncharacterized protein LOC101217233 [Cucumis sativus] >KAE8652022.1 hypothetical protein Csa_004745 [Cucumis sativus])

HSP 1 Score: 586 bits (1510), Expect = 1.04e-208
Identity = 293/361 (81.16%), Postives = 325/361 (90.03%), Query Frame = 0

Query: 1   MRLSIARSYLVAPHRVIPSRPIFSSSLKPQIPLFLRPPNYSTTLSDLQKWHDWAKTLNSS 60
           MRLSIAR+YLVAPHR    RPI SSSL PQIPLFLRPPNYS TL D  KWH+WAK LN S
Sbjct: 1   MRLSIARAYLVAPHRAKSIRPICSSSLNPQIPLFLRPPNYSVTLKDFHKWHNWAKILNCS 60

Query: 61  VGSSFLEADNGPDSTLLNRELKWLIEDAVEEESKSLSSELGIDDVKRNREQGLRNVRLKV 120
           VGSSF++ DNGPDSTLL+RELKWL++DAVE+  KSLSSEL  +++++N E GLRNVRLKV
Sbjct: 61  VGSSFVDTDNGPDSTLLHRELKWLVQDAVED--KSLSSELE-NEIEQNPELGLRNVRLKV 120

Query: 121 GIEELYRLWKQRIHERRPFQYIVGCEHWRDLVLSVEEGVLIPRPETELLVDLVAKVISDK 180
           GIEELYRLWKQRIHERRPFQYIVGCEHWRDL+LSVEEGVLIPRPETE+LVDLV KV+SD 
Sbjct: 121 GIEELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDN 180

Query: 181 EALGGEGFWVDLGTGSGAIAIGIGRILDGRARVIATDVSPTALAVAAYNVQRYGLQELIE 240
           EAL  EG WVDLGTGSGAIAIGI RIL+GR RVIATD+S  ALAVA YNVQRYGLQ+LIE
Sbjct: 181 EALR-EGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQDLIE 240

Query: 241 LRQGSWYEPLKDVQGRVSGIISNPPYIPSDNIFGLQAEVCKHEPRVALDGGTNGMDELIH 300
           LRQGSWYEPL+DVQG++SGIISNPPYIPSDNIFGLQAEV KHEPRVALDGGTNGMDELIH
Sbjct: 241 LRQGSWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIH 300

Query: 301 LCDEAALMLKPGGFFAFETNGEDQCKNLVKYMESIHRGRFCHLEMVSDFAAIPRFVTGFL 360
           LCDEA +MLK GGF AFETNGEDQCK+LV YME+ H+G+FC+L++VSDFA+IPRF+TGFL
Sbjct: 301 LCDEATVMLKLGGFLAFETNGEDQCKHLVNYMENNHKGKFCNLKIVSDFASIPRFITGFL 357

BLAST of MC11g0744 vs. NCBI nr
Match: XP_008444272.1 (PREDICTED: release factor glutamine methyltransferase [Cucumis melo] >KAA0052296.1 release factor glutamine methyltransferase [Cucumis melo var. makuwa] >TYK01861.1 release factor glutamine methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 585 bits (1508), Expect = 2.17e-208
Identity = 294/362 (81.22%), Postives = 329/362 (90.88%), Query Frame = 0

Query: 1   MRLSIARSYLVAPHRVIPSRPIFSSSL-KPQIPLFLRPPNYSTTLSDLQKWHDWAKTLNS 60
           MRLSIAR+YLVAPHR    RPI SSS   PQIPLFLRPPNYS TL+D+ KWH+WAKTLNS
Sbjct: 1   MRLSIARAYLVAPHRAKSIRPICSSSSPNPQIPLFLRPPNYSVTLTDVHKWHNWAKTLNS 60

Query: 61  SVGSSFLEADNGPDSTLLNRELKWLIEDAVEEESKSLSSELGIDDVKRNREQGLRNVRLK 120
           SVGSSF+  DNGPDSTLL+RELKWLI+DAV++  KSLSSEL  +++++  E GLRNVRLK
Sbjct: 61  SVGSSFVHTDNGPDSTLLHRELKWLIQDAVDD--KSLSSELE-NEIEQYPELGLRNVRLK 120

Query: 121 VGIEELYRLWKQRIHERRPFQYIVGCEHWRDLVLSVEEGVLIPRPETELLVDLVAKVISD 180
           VGI+ELYRLWKQRIH+RRPFQYIVGCEHWRDL+LSVEEGVLIPRPETE+LVDLV KV+SD
Sbjct: 121 VGIDELYRLWKQRIHDRRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSD 180

Query: 181 KEALGGEGFWVDLGTGSGAIAIGIGRILDGRARVIATDVSPTALAVAAYNVQRYGLQELI 240
            EAL  EG WVDLGTGSGAIAIGI RIL+GR RVIATD+S  ALAVA YNVQRYGLQ+LI
Sbjct: 181 NEALR-EGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSTIALAVAGYNVQRYGLQDLI 240

Query: 241 ELRQGSWYEPLKDVQGRVSGIISNPPYIPSDNIFGLQAEVCKHEPRVALDGGTNGMDELI 300
           ELRQGSWYEPL+DVQG++SGIISNPPYIPSDNIFGLQAEV KHEPRVALDGGTNGMDELI
Sbjct: 241 ELRQGSWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELI 300

Query: 301 HLCDEAALMLKPGGFFAFETNGEDQCKNLVKYMESIHRGRFCHLEMVSDFAAIPRFVTGF 360
           HLCDEAA+MLKPGGFFAFETNGEDQCK+LV YME+ H+G+FC+L++VSDFA+IPRF+TGF
Sbjct: 301 HLCDEAAVMLKPGGFFAFETNGEDQCKHLVNYMENSHKGKFCNLKIVSDFASIPRFITGF 358

BLAST of MC11g0744 vs. ExPASy TrEMBL
Match: A0A6J1CGS6 (uncharacterized protein LOC111011525 OS=Momordica charantia OX=3673 GN=LOC111011525 PE=4 SV=1)

HSP 1 Score: 708 bits (1828), Expect = 1.59e-257
Identity = 355/362 (98.07%), Postives = 357/362 (98.62%), Query Frame = 0

Query: 1   MRLSIARSYLVAPHRVIPSRPIFSSSLKPQIPLFLRPPNYSTTLSDLQKWHDWAKTLNSS 60
           MRLSIARSYLVAPHRVIPSRPIFSSSLKPQIPLFLRPPNYSTTLSD+QKWHDWAKTLNSS
Sbjct: 1   MRLSIARSYLVAPHRVIPSRPIFSSSLKPQIPLFLRPPNYSTTLSDVQKWHDWAKTLNSS 60

Query: 61  VGSSFLEADNGPDSTLLNRELKWLIEDAVEEESKSLSSELGIDDVKRNREQGLRNVRLKV 120
           VGSSFLEADNGPDSTLLNRELKWLIEDAVEEESKSLSSELGIDDVKRNREQGLRNVRLKV
Sbjct: 61  VGSSFLEADNGPDSTLLNRELKWLIEDAVEEESKSLSSELGIDDVKRNREQGLRNVRLKV 120

Query: 121 GIEELYRLWKQRIHERRPFQYIVGCEHWRDLVLSVEEGVLIPRPETELLVDLVAKVISDK 180
           GIEELYRLWKQRIHERRPFQYIVGCEHWRDLVLSVEEGVLIPRPETELLVDL     +DK
Sbjct: 121 GIEELYRLWKQRIHERRPFQYIVGCEHWRDLVLSVEEGVLIPRPETELLVDL-----ADK 180

Query: 181 EALGGEGFWVDLGTGSGAIAIGIGRILDGRARVIATDVSPTALAVAAYNVQRYGLQELIE 240
           EALGGEGFWVDLGTGSGAIAIGIGRILDGRARVIATDVSPTALAVAAYNVQRYGLQELIE
Sbjct: 181 EALGGEGFWVDLGTGSGAIAIGIGRILDGRARVIATDVSPTALAVAAYNVQRYGLQELIE 240

Query: 241 LRQGSWYEPLKDVQGRVSGIISNPPYIPSDNIFGLQAEVCKHEPRVALDGGTNGMDELIH 300
           LRQGSWYEPLKDVQGRVSGIISNPPYIPSDNIFGLQAEVCKHEPRVALDGGTNGMDELIH
Sbjct: 241 LRQGSWYEPLKDVQGRVSGIISNPPYIPSDNIFGLQAEVCKHEPRVALDGGTNGMDELIH 300

Query: 301 LCDEAALMLKPGGFFAFETNGEDQCKNLVKYMESIHRGRFCHLEMVSDFAAIPRFVTGFL 360
           LCDEAALMLKPGGFFAFETNGEDQCKNLVKYMESIHRGRFCHLEMVSDFAAIPRFVTGFL
Sbjct: 301 LCDEAALMLKPGGFFAFETNGEDQCKNLVKYMESIHRGRFCHLEMVSDFAAIPRFVTGFL 357

Query: 361 KE 362
           KE
Sbjct: 361 KE 357

BLAST of MC11g0744 vs. ExPASy TrEMBL
Match: A0A6J1F974 (uncharacterized protein LOC111443257 OS=Cucurbita moschata OX=3662 GN=LOC111443257 PE=4 SV=1)

HSP 1 Score: 590 bits (1520), Expect = 2.62e-210
Identity = 296/362 (81.77%), Postives = 327/362 (90.33%), Query Frame = 0

Query: 1   MRLSIARSYLVAPHRVIPSRPIFSSSLKPQIPLFLRPPNYSTTLSDLQKWHDWAKTLNSS 60
           MRLSI+R+YL AP RV PSR I S+S KP+IPLFLRPPNYS TL+DLQKWHDWAKTL+SS
Sbjct: 16  MRLSISRAYLAAPRRVRPSRSICSASSKPRIPLFLRPPNYSATLTDLQKWHDWAKTLSSS 75

Query: 61  VGSSFLEADNGPDSTLLNRELKWLIEDAVEEESKSLSSELGIDDVKRNREQGLRNVRLKV 120
           VGSSF++ADNGPDS LL+RELKWLIEDAVE+  KS+SSEL  +  ++N E   RNVRLKV
Sbjct: 76  VGSSFVDADNGPDSALLHRELKWLIEDAVED--KSVSSELRTNS-EQNHEHNHRNVRLKV 135

Query: 121 GIEELYRLWKQRIHERRPFQYIVGCEHWRDLVLSVEEGVLIPRPETELLVDLVAKVISDK 180
           GIEELY +WKQRIHERRPFQYIVGCEHWRDL+LSVEEGVLIPRPETE+LVDLVA+V+SD 
Sbjct: 136 GIEELYGVWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVAEVVSDN 195

Query: 181 EALGGEGFWVDLGTGSGAIAIGIGRILDGRARVIATDVSPTALAVAAYNVQRYGLQELIE 240
           EAL  EG WVDLGTGSGAIAI I R+L+GRARVIATD+SPTAL VA YNVQRYGLQ+ IE
Sbjct: 196 EALR-EGLWVDLGTGSGAIAIAICRVLEGRARVIATDLSPTALTVAGYNVQRYGLQDSIE 255

Query: 241 LRQGSWYEPLKDVQGRVSGIISNPPYIPSDNIFGLQAEVCKHEPRVALDGGTNGMDELIH 300
           LRQGSWYEPLKDVQG++SGIISNPPYIPSDNI GLQAEV KHEPRVALDGGTNGMDELIH
Sbjct: 256 LRQGSWYEPLKDVQGKLSGIISNPPYIPSDNIVGLQAEVGKHEPRVALDGGTNGMDELIH 315

Query: 301 LCDEAALMLKPGGFFAFETNGEDQCKNLVKYMESIHRGRFCHLEMVSDFAAIPRFVTGFL 360
           LCDEAA+MLKPGGFFAFETNGEDQCK+LVKYME  HRGR C+L++VSDFA IPRF+TGFL
Sbjct: 316 LCDEAAVMLKPGGFFAFETNGEDQCKHLVKYMEDNHRGRLCNLKIVSDFAGIPRFITGFL 373

Query: 361 KE 362
            E
Sbjct: 376 NE 373

BLAST of MC11g0744 vs. ExPASy TrEMBL
Match: A0A0A0L8I9 (MTS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G524590 PE=4 SV=1)

HSP 1 Score: 586 bits (1510), Expect = 5.02e-209
Identity = 293/361 (81.16%), Postives = 325/361 (90.03%), Query Frame = 0

Query: 1   MRLSIARSYLVAPHRVIPSRPIFSSSLKPQIPLFLRPPNYSTTLSDLQKWHDWAKTLNSS 60
           MRLSIAR+YLVAPHR    RPI SSSL PQIPLFLRPPNYS TL D  KWH+WAK LN S
Sbjct: 1   MRLSIARAYLVAPHRAKSIRPICSSSLNPQIPLFLRPPNYSVTLKDFHKWHNWAKILNCS 60

Query: 61  VGSSFLEADNGPDSTLLNRELKWLIEDAVEEESKSLSSELGIDDVKRNREQGLRNVRLKV 120
           VGSSF++ DNGPDSTLL+RELKWL++DAVE+  KSLSSEL  +++++N E GLRNVRLKV
Sbjct: 61  VGSSFVDTDNGPDSTLLHRELKWLVQDAVED--KSLSSELE-NEIEQNPELGLRNVRLKV 120

Query: 121 GIEELYRLWKQRIHERRPFQYIVGCEHWRDLVLSVEEGVLIPRPETELLVDLVAKVISDK 180
           GIEELYRLWKQRIHERRPFQYIVGCEHWRDL+LSVEEGVLIPRPETE+LVDLV KV+SD 
Sbjct: 121 GIEELYRLWKQRIHERRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSDN 180

Query: 181 EALGGEGFWVDLGTGSGAIAIGIGRILDGRARVIATDVSPTALAVAAYNVQRYGLQELIE 240
           EAL  EG WVDLGTGSGAIAIGI RIL+GR RVIATD+S  ALAVA YNVQRYGLQ+LIE
Sbjct: 181 EALR-EGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSSIALAVAGYNVQRYGLQDLIE 240

Query: 241 LRQGSWYEPLKDVQGRVSGIISNPPYIPSDNIFGLQAEVCKHEPRVALDGGTNGMDELIH 300
           LRQGSWYEPL+DVQG++SGIISNPPYIPSDNIFGLQAEV KHEPRVALDGGTNGMDELIH
Sbjct: 241 LRQGSWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELIH 300

Query: 301 LCDEAALMLKPGGFFAFETNGEDQCKNLVKYMESIHRGRFCHLEMVSDFAAIPRFVTGFL 360
           LCDEA +MLK GGF AFETNGEDQCK+LV YME+ H+G+FC+L++VSDFA+IPRF+TGFL
Sbjct: 301 LCDEATVMLKLGGFLAFETNGEDQCKHLVNYMENNHKGKFCNLKIVSDFASIPRFITGFL 357

BLAST of MC11g0744 vs. ExPASy TrEMBL
Match: A0A5A7UDM4 (Release factor glutamine methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold113G001190 PE=4 SV=1)

HSP 1 Score: 585 bits (1508), Expect = 1.05e-208
Identity = 294/362 (81.22%), Postives = 329/362 (90.88%), Query Frame = 0

Query: 1   MRLSIARSYLVAPHRVIPSRPIFSSSL-KPQIPLFLRPPNYSTTLSDLQKWHDWAKTLNS 60
           MRLSIAR+YLVAPHR    RPI SSS   PQIPLFLRPPNYS TL+D+ KWH+WAKTLNS
Sbjct: 1   MRLSIARAYLVAPHRAKSIRPICSSSSPNPQIPLFLRPPNYSVTLTDVHKWHNWAKTLNS 60

Query: 61  SVGSSFLEADNGPDSTLLNRELKWLIEDAVEEESKSLSSELGIDDVKRNREQGLRNVRLK 120
           SVGSSF+  DNGPDSTLL+RELKWLI+DAV++  KSLSSEL  +++++  E GLRNVRLK
Sbjct: 61  SVGSSFVHTDNGPDSTLLHRELKWLIQDAVDD--KSLSSELE-NEIEQYPELGLRNVRLK 120

Query: 121 VGIEELYRLWKQRIHERRPFQYIVGCEHWRDLVLSVEEGVLIPRPETELLVDLVAKVISD 180
           VGI+ELYRLWKQRIH+RRPFQYIVGCEHWRDL+LSVEEGVLIPRPETE+LVDLV KV+SD
Sbjct: 121 VGIDELYRLWKQRIHDRRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSD 180

Query: 181 KEALGGEGFWVDLGTGSGAIAIGIGRILDGRARVIATDVSPTALAVAAYNVQRYGLQELI 240
            EAL  EG WVDLGTGSGAIAIGI RIL+GR RVIATD+S  ALAVA YNVQRYGLQ+LI
Sbjct: 181 NEALR-EGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSTIALAVAGYNVQRYGLQDLI 240

Query: 241 ELRQGSWYEPLKDVQGRVSGIISNPPYIPSDNIFGLQAEVCKHEPRVALDGGTNGMDELI 300
           ELRQGSWYEPL+DVQG++SGIISNPPYIPSDNIFGLQAEV KHEPRVALDGGTNGMDELI
Sbjct: 241 ELRQGSWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELI 300

Query: 301 HLCDEAALMLKPGGFFAFETNGEDQCKNLVKYMESIHRGRFCHLEMVSDFAAIPRFVTGF 360
           HLCDEAA+MLKPGGFFAFETNGEDQCK+LV YME+ H+G+FC+L++VSDFA+IPRF+TGF
Sbjct: 301 HLCDEAAVMLKPGGFFAFETNGEDQCKHLVNYMENSHKGKFCNLKIVSDFASIPRFITGF 358

BLAST of MC11g0744 vs. ExPASy TrEMBL
Match: A0A1S3BA23 (release factor glutamine methyltransferase OS=Cucumis melo OX=3656 GN=LOC103487646 PE=4 SV=1)

HSP 1 Score: 585 bits (1508), Expect = 1.05e-208
Identity = 294/362 (81.22%), Postives = 329/362 (90.88%), Query Frame = 0

Query: 1   MRLSIARSYLVAPHRVIPSRPIFSSSL-KPQIPLFLRPPNYSTTLSDLQKWHDWAKTLNS 60
           MRLSIAR+YLVAPHR    RPI SSS   PQIPLFLRPPNYS TL+D+ KWH+WAKTLNS
Sbjct: 1   MRLSIARAYLVAPHRAKSIRPICSSSSPNPQIPLFLRPPNYSVTLTDVHKWHNWAKTLNS 60

Query: 61  SVGSSFLEADNGPDSTLLNRELKWLIEDAVEEESKSLSSELGIDDVKRNREQGLRNVRLK 120
           SVGSSF+  DNGPDSTLL+RELKWLI+DAV++  KSLSSEL  +++++  E GLRNVRLK
Sbjct: 61  SVGSSFVHTDNGPDSTLLHRELKWLIQDAVDD--KSLSSELE-NEIEQYPELGLRNVRLK 120

Query: 121 VGIEELYRLWKQRIHERRPFQYIVGCEHWRDLVLSVEEGVLIPRPETELLVDLVAKVISD 180
           VGI+ELYRLWKQRIH+RRPFQYIVGCEHWRDL+LSVEEGVLIPRPETE+LVDLV KV+SD
Sbjct: 121 VGIDELYRLWKQRIHDRRPFQYIVGCEHWRDLILSVEEGVLIPRPETEVLVDLVEKVVSD 180

Query: 181 KEALGGEGFWVDLGTGSGAIAIGIGRILDGRARVIATDVSPTALAVAAYNVQRYGLQELI 240
            EAL  EG WVDLGTGSGAIAIGI RIL+GR RVIATD+S  ALAVA YNVQRYGLQ+LI
Sbjct: 181 NEALR-EGLWVDLGTGSGAIAIGICRILEGRGRVIATDLSTIALAVAGYNVQRYGLQDLI 240

Query: 241 ELRQGSWYEPLKDVQGRVSGIISNPPYIPSDNIFGLQAEVCKHEPRVALDGGTNGMDELI 300
           ELRQGSWYEPL+DVQG++SGIISNPPYIPSDNIFGLQAEV KHEPRVALDGGTNGMDELI
Sbjct: 241 ELRQGSWYEPLQDVQGKLSGIISNPPYIPSDNIFGLQAEVGKHEPRVALDGGTNGMDELI 300

Query: 301 HLCDEAALMLKPGGFFAFETNGEDQCKNLVKYMESIHRGRFCHLEMVSDFAAIPRFVTGF 360
           HLCDEAA+MLKPGGFFAFETNGEDQCK+LV YME+ H+G+FC+L++VSDFA+IPRF+TGF
Sbjct: 301 HLCDEAAVMLKPGGFFAFETNGEDQCKHLVNYMENSHKGKFCNLKIVSDFASIPRFITGF 358

BLAST of MC11g0744 vs. TAIR 10
Match: AT5G64150.1 (RNA methyltransferase family protein )

HSP 1 Score: 402.1 bits (1032), Expect = 4.6e-112
Identity = 199/342 (58.19%), Postives = 259/342 (75.73%), Query Frame = 0

Query: 19  SRPIFSSSLKPQIPLFLRPPNYSTTLSDLQKWHDWAKTLNSSVGSSFLEADNGPDSTLLN 78
           SRP  S S+ P+ PL+LR P+++T+LS++ KWHDWAK L SSV  S   +++  DS +L+
Sbjct: 41  SRPTKSLSITPKTPLYLRTPSHATSLSEVWKWHDWAKDLASSVEESSKNSEDTLDSVILH 100

Query: 79  RELKWLIEDAVEEESKSLSSELGIDDVKRNREQGLRNVRLKVGIEELYRLWKQRIHERRP 138
           RELKWLIED++ ++   +     I D       G +NV+L+  +EELY LW+QRI +RRP
Sbjct: 101 RELKWLIEDSIVDDPLVILHRSEIAD------NGEKNVKLRASLEELYDLWRQRIEKRRP 160

Query: 139 FQYIVGCEHWRDLVLSVEEGVLIPRPETELLVDLVAKVISDKEALGGEGFWVDLGTGSGA 198
           FQY+VGCEHWRDLVL VEEGVLIPRPETEL+VD+V ++++  E    +  W DLGTGSGA
Sbjct: 161 FQYVVGCEHWRDLVLCVEEGVLIPRPETELIVDMVEELVTRDEWF-KKDIWADLGTGSGA 220

Query: 199 IAIGIGRILDGRARVIATDVSPTALAVAAYNVQRYGLQELIELRQGSWYEPLKDVQGRVS 258
           IAIGI ++L  R RVIATD+SP A+AVA +NVQRY L+ +IE+R+GSW+EPLKD++G++ 
Sbjct: 221 IAIGIAKVLGSRGRVIATDLSPVAIAVAGHNVQRYSLEGMIEVREGSWFEPLKDLEGKLV 280

Query: 259 GIISNPPYIPSDNIFGLQAEVCKHEPRVALDGGTNGMDELIHLCDEAALMLKPGGFFAFE 318
           G++SNPPYIPSD+I GLQAEV +HEP++ALDGG +G D L HLC  A+ ML+PGGFF FE
Sbjct: 281 GLVSNPPYIPSDDIPGLQAEVGRHEPKLALDGGIDGTDSLFHLCHGASRMLQPGGFFVFE 340

Query: 319 TNGEDQCKNLVKY-MESIHRGRFCHLEMVSDFAAIPRFVTGF 360
           TNGE Q K +V Y M S  +  F  L++VSDFA I RFVTGF
Sbjct: 341 TNGEKQSKMIVDYMMTSDLKDCFSDLKIVSDFAGINRFVTGF 375

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P740032.5e-4638.13Release factor glutamine methyltransferase OS=Synechocystis sp. (strain PCC 6803... [more]
Q8DHV79.0e-4440.77Release factor glutamine methyltransferase OS=Thermosynechococcus elongatus (str... [more]
Q7NJS73.4e-4341.80Release factor glutamine methyltransferase OS=Gloeobacter violaceus (strain ATCC... [more]
Q7VDL79.3e-3335.12Release factor glutamine methyltransferase OS=Prochlorococcus marinus (strain SA... [more]
Q2RFW11.3e-3137.24Release factor glutamine methyltransferase OS=Moorella thermoacetica (strain ATC... [more]
Match NameE-valueIdentityDescription
XP_022141020.13.28e-25798.07uncharacterized protein LOC111011525 [Momordica charantia][more]
XP_022936763.15.40e-21081.77uncharacterized protein LOC111443257 [Cucurbita moschata] >XP_022936764.1 unchar... [more]
XP_023534998.16.87e-21082.04uncharacterized protein LOC111796557 [Cucurbita pepo subsp. pepo][more]
XP_004151022.11.04e-20881.16uncharacterized protein LOC101217233 [Cucumis sativus] >KAE8652022.1 hypothetica... [more]
XP_008444272.12.17e-20881.22PREDICTED: release factor glutamine methyltransferase [Cucumis melo] >KAA0052296... [more]
Match NameE-valueIdentityDescription
A0A6J1CGS61.59e-25798.07uncharacterized protein LOC111011525 OS=Momordica charantia OX=3673 GN=LOC111011... [more]
A0A6J1F9742.62e-21081.77uncharacterized protein LOC111443257 OS=Cucurbita moschata OX=3662 GN=LOC1114432... [more]
A0A0A0L8I95.02e-20981.16MTS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G524590 PE=4 SV... [more]
A0A5A7UDM41.05e-20881.22Release factor glutamine methyltransferase OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3BA231.05e-20881.22release factor glutamine methyltransferase OS=Cucumis melo OX=3656 GN=LOC1034876... [more]
Match NameE-valueIdentityDescription
AT5G64150.14.6e-11258.19RNA methyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007848Methyltransferase small domainPFAMPF05175MTScoord: 187..266
e-value: 7.4E-9
score: 35.4
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 153..359
e-value: 2.3E-49
score: 170.0
NoneNo IPR availablePANTHERPTHR47441FAMILY NOT NAMEDcoord: 19..359
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 190..269
e-value: 2.95541E-10
score: 55.1286
IPR004556Methyltransferase HemK-likeTIGRFAMTIGR00536TIGR00536coord: 82..361
e-value: 4.4E-55
score: 184.7
IPR002052DNA methylase, N-6 adenine-specific, conserved sitePROSITEPS00092N6_MTASEcoord: 260..266
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 123..334

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC11g0744.1MC11g0744.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006479 protein methylation
biological_process GO:0032259 methylation
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008276 protein methyltransferase activity
molecular_function GO:0008168 methyltransferase activity