MC11g0709 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g0709 vs. ExPASy Swiss-Prot
Match: Q9M9A8 (Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.8e-76
Identity = 159/399 (39.85%), Postives = 231/399 (57.89%), Query Frame = 0

Query: 74  ASVLFSVQGNVYPLGYFTVSINIGNPP--KIFELDIDTGSDLTWVQCDAPCTGCTLPRDK 133
           ++ +F V GNVYP G +   I +G P   + + LDIDTGS+LTW+QCDAPCT C    ++
Sbjct: 187 STTIFPVGGNVYPDGLYYTRILVGKPEDGQYYHLDIDTGSELTWIQCDAPCTSCAKGANQ 246

Query: 134 QYRPH-NNAVRCGEPLCAALVSPGQVP--CNNPNDQCDYEVEYADHGSSTGVLVRDLVPL 193
            Y+P  +N VR  E  C   V   Q+   C N + QCDYE+EYADH  S GVL +D   L
Sbjct: 247 LYKPRKDNLVRSSEAFCVE-VQRNQLTEHCENCH-QCDYEIEYADHSYSMGVLTKDKFHL 306

Query: 194 RFVNGAFLTPNLGFGCGYEQRNGGLQPSSSIAGVLGLGRSEASIVAQLSALGLVSNIIGH 253
           +  NG+    ++ FGCGY+Q+   L       G+LGL R++ S+ +QL++ G++SN++GH
Sbjct: 307 KLHNGSLAESDIVFGCGYDQQGLLLNTLLKTDGILGLSRAKISLPSQLASRGIISNVVGH 366

Query: 254 CLSG--QGGGFLFFGGDLFPSSKMSWTPILRTPG-GNYSVGPAEVYYGGKAVGSEGLT-- 313
           CL+    G G++F G DL PS  M+W P+L       Y +   ++ YG   +  +G    
Sbjct: 367 CLASDLNGEGYIFMGSDLVPSHGMTWVPMLHDSRLDAYQMQVTKMSYGQGMLSLDGENGR 426

Query: 314 ---LTFDSGSSYTYFNSKVYGAVEKLLKNDLKGKLRDEPEDETLPVCW--KGTKAFKSVA 373
              + FD+GSSYTYF ++ Y  +   L+     +L  +  DETLP+CW  K    F S++
Sbjct: 427 VGKVLFDTGSSYTYFPNQAYSQLVTSLQEVSGLELTRDDSDETLPICWRAKTNFPFSSLS 486

Query: 374 DVRNFFKPLALGFTNS----KNQFQIPPEAYLIISKFGNVCLGISNGSQVGLGNDNLIGD 433
           DV+ FF+P+ L   +       +  I PE YLIIS  GNVCLGI +GS V  G+  ++GD
Sbjct: 487 DVKKFFRPITLQIGSKWLIISRKLLIQPEDYLIISNKGNVCLGILDGSSVHDGSTIILGD 546

Query: 434 ISMLDKIVIYDNESQRIGWAPANC-NKRPMKHGEQWLAG 453
           ISM   +++YDN  +RIGW  ++C   R + H   +  G
Sbjct: 547 ISMRGHLIVYDNVKRRIGWMKSDCVRPREIDHNVPFFQG 583

BLAST of MC11g0709 vs. ExPASy Swiss-Prot
Match: Q0IU52 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. japonica OX=39947 GN=ASP1 PE=2 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 9.8e-75
Identity = 153/382 (40.05%), Postives = 230/382 (60.21%), Query Frame = 0

Query: 74  ASVLFSVQGNVYPLGYFTVSINIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQY 133
           ++V+  + GNVYP+G+F +++NIG+P K + LDIDTGS LTW+QCDAPCT C +     Y
Sbjct: 22  SAVVLELHGNVYPIGHFFITMNIGDPAKSYFLDIDTGSTLTWLQCDAPCTNCNIVPHVLY 81

Query: 134 RP-HNNAVRCGEPLCAALVSP-GQVPCNNPNDQCDYEVEYADHGSSTGVLVRDLVPLRFV 193
           +P     V C + LC  L +  G+        QCDY ++Y D  SS GVLV D   L   
Sbjct: 82  KPTPKKLVTCADSLCTDLYTDLGKPKRCGSQKQCDYVIQYVD-SSSMGVLVIDRFSLSAS 141

Query: 194 NGAFLTPNLGFGCGYEQRNGGLQPSSSIAGVLGLGRSEASIVAQLSALGLVS-NIIGHCL 253
           NG   T  + FGCGY+Q          +  +LGL R + ++++QL + G+++ +++GHC+
Sbjct: 142 NGTNPT-TIAFGCGYDQGKKNRNVPIPVDSILGLSRGKVTLLSQLKSQGVITKHVLGHCI 201

Query: 254 SGQGGGFLFFGGDLFPSSKMSWTPILRTPGGNYSVGPAEVYY--GGKAVGSEGLTLTFDS 313
           S +GGGFLFFG    P+S ++WTP+ R     YS G   +++    KA+ +  + + FDS
Sbjct: 202 SSKGGGFLFFGDAQVPTSGVTWTPMNR-EHKYYSPGHGTLHFDSNSKAISAAPMAVIFDS 261

Query: 314 GSSYTYFNSKVYGAVEKLLKNDLKGKLRDEPE----DETLPVCWKGTKAFKSVADVRNFF 373
           G++YTYF ++ Y A   ++K+ L  + +   E    D  L VCWKG     ++ +V+  F
Sbjct: 262 GATYTYFAAQPYQATLSVVKSTLNSECKFLTEVTEKDRALTVCWKGKDKIVTIDEVKKCF 321

Query: 374 KPLALGFT--NSKNQFQIPPEAYLIISKFGNVCLGISNGSQ--VGLGNDNLIGDISMLDK 433
           + L+L F   + K   +IPPE YLIIS+ G+VCLGI +GS+  + L   NLIG I+MLD+
Sbjct: 322 RSLSLEFADGDKKATLEIPPEHYLIISQEGHVCLGILDGSKEHLSLAGTNLIGGITMLDQ 381

Query: 434 IVIYDNESQRIGWAPANCNKRP 443
           +VIYD+E   +GW    C++ P
Sbjct: 382 MVIYDSERSLLGWVNYQCDRIP 400

BLAST of MC11g0709 vs. ExPASy Swiss-Prot
Match: A2ZC67 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=2)

HSP 1 Score: 275.4 bits (703), Expect = 1.2e-72
Identity = 152/382 (39.79%), Postives = 228/382 (59.69%), Query Frame = 0

Query: 74  ASVLFSVQGNVYPLGYFTVSINIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQY 133
           ++V+  + GNVYP+G+F V++NIG+P K + LDIDTGS LTW+QCD PC  C       Y
Sbjct: 22  SAVVLELHGNVYPIGHFFVTMNIGDPAKPYFLDIDTGSTLTWLQCDYPCINCNKVPHGLY 81

Query: 134 RPH-NNAVRCGEPLCAALVSPGQVPCN-NPNDQCDYEVEYADHGSSTGVLVRDLVPLRFV 193
           +P    AV+C E  CA L +  + P    P +QC Y ++Y   GSS GVL+ D   L   
Sbjct: 82  KPELKYAVKCTEQRCADLYADLRKPMKCGPKNQCHYGIQYVG-GSSIGVLIVDSFSLPAS 141

Query: 194 NGAFLTPNLGFGCGYEQRNGGLQPSSSIAGVLGLGRSEASIVAQLSALGLVS-NIIGHCL 253
           NG   T ++ FGCGY Q        + + G+LGLGR + ++++QL + G+++ +++GHC+
Sbjct: 142 NGTNPT-SIAFGCGYNQGKNNHNVPTPVNGILGLGRGKVTLLSQLKSQGVITKHVLGHCI 201

Query: 254 SGQGGGFLFFGGDLFPSSKMSWTPILRTPGGNYS--VGPAEVYYGGKAVGSEGLTLTFDS 313
           S +G GFLFFG    P+S ++W+P+ R    +YS   G  +     K + +  + + FDS
Sbjct: 202 SSKGKGFLFFGDAKVPTSGVTWSPMNR-EHKHYSPRQGTLQFNSNSKPISAAPMEVIFDS 261

Query: 314 GSSYTYFNSKVYGA----VEKLLKNDLKGKLRDEPEDETLPVCWKGTKAFKSVADVRNFF 373
           G++YTYF  + Y A    V+  L  + K     + +D  L VCWKG    +++ +V+  F
Sbjct: 262 GATYTYFALQPYHATLSVVKSTLSKECKFLTEVKEKDRALTVCWKGKDKIRTIDEVKKCF 321

Query: 374 KPLALGFT--NSKNQFQIPPEAYLIISKFGNVCLGISNGSQ--VGLGNDNLIGDISMLDK 433
           + L+L F   + K   +IPPE YLIIS+ G+VCLGI +GS+    L   NLIG I+MLD+
Sbjct: 322 RSLSLKFADGDKKATLEIPPEHYLIISQEGHVCLGILDGSKEHPSLAGTNLIGGITMLDQ 381

Query: 434 IVIYDNESQRIGWAPANCNKRP 443
           +VIYD+E   +GW    C++ P
Sbjct: 382 MVIYDSERSLLGWVNYQCDRIP 400

BLAST of MC11g0709 vs. ExPASy Swiss-Prot
Match: Q4V3D2 (Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.4e-33
Identity = 112/434 (25.81%), Postives = 192/434 (44.24%), Query Frame = 0

Query: 39  LLLLIVSVLFANFAVSLSDKFS--------VANWGQLEADRFGASVLFSVQGN--VYPLG 98
           + +L++ V+  NF  +++ KF+        + +       R  A++   + G+     +G
Sbjct: 17  VFVLVIQVVSGNFVFNVTHKFAGKEKQLSELKSHDSFRHARMLANIDLPLGGDSRADSIG 76

Query: 99  YFTVSINIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPR---------DKQYRPHNNA 158
            +   I +G+PPK + + +DTGSD+ WV C APC  C +           D +    +  
Sbjct: 77  LYFTKIKLGSPPKEYYVQVDTGSDILWVNC-APCPKCPVKTDLGIPLSLYDSKTSSTSKN 136

Query: 159 VRCGEPLCAALVSPGQVPCNNPNDQCDYEVEYADHGSSTGVLVRDLVPLRFVNGAFLTPN 218
           V C +  C+ ++         P   C Y V Y D  +S G  ++D + L  V G   T  
Sbjct: 137 VGCEDDFCSFIMQSETCGAKKP---CSYHVVYGDGSTSDGDFIKDNITLEQVTGNLRTAP 196

Query: 219 LG----FGCGYEQRNGGLQPSSSIAGVLGLGRSEASIVAQLSALGLVSNIIGHCLSGQGG 278
           L     FGCG  Q     Q  S++ G++G G+S  SI++QL+A G    I  HCL    G
Sbjct: 197 LAQEVVFGCGKNQSGQLGQTDSAVDGIMGFGQSNTSIISQLAAGGSTKRIFSHCLDNMNG 256

Query: 279 GFLFFGGDLFPSSKMSWTPILRT--------PGGNYSVGPAEVYYGGKAVGSEGLTLTFD 338
           G +F  G++  S  +  TPI+           G +    P ++     +   +G T+  D
Sbjct: 257 GGIFAVGEV-ESPVVKTTPIVPNQVHYNVILKGMDVDGDPIDLPPSLASTNGDGGTI-ID 316

Query: 339 SGSSYTYFNSKVYGAVEKLLKNDLKGKLRDEPEDETLPVCWKGTKAFKSVADVRNFFKPL 398
           SG++  Y    +Y ++  + K   K +++     ET          F   ++    F  +
Sbjct: 317 SGTTLAYLPQNLYNSL--IEKITAKQQVKLHMVQETF-------ACFSFTSNTDKAFPVV 376

Query: 399 ALGFTNSKNQFQIPPEAYLIISKFGNVCLGISNGSQVGLGNDN--LIGDISMLDKIVIYD 440
            L F +S  +  + P  YL   +    C G  +G        +  L+GD+ + +K+V+YD
Sbjct: 377 NLHFEDSL-KLSVYPHDYLFSLREDMYCFGWQSGGMTTQDGADVILLGDLVLSNKLVVYD 434

BLAST of MC11g0709 vs. ExPASy Swiss-Prot
Match: Q9S9K4 (Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2)

HSP 1 Score: 145.6 bits (366), Expect = 1.4e-33
Identity = 116/444 (26.13%), Postives = 194/444 (43.69%), Query Frame = 0

Query: 39  LLLLIVSVLFANFAVSLSDKFS--------VANWGQLEADRFGASVLFSVQGN--VYPLG 98
           + ++++    ANF      KF+          +       R  AS+   + G+  V  +G
Sbjct: 13  VFVIVIEFASANFVFKAQHKFAGKKKNLEHFKSHDTRRHSRMLASIDLPLGGDSRVDSVG 72

Query: 99  YFTVSINIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYR---------PHNNA 158
            +   I +G+PPK + + +DTGSD+ W+ C  PC  C    +  +R           +  
Sbjct: 73  LYFTKIKLGSPPKEYHVQVDTGSDILWINC-KPCPKCPTKTNLNFRLSLFDMNASSTSKK 132

Query: 159 VRCGEPLCAALVSPGQVPCNNPNDQCDYEVEYADHGSSTGVLVRDLVPLRFVNGAFLTPN 218
           V C +  C+ +    Q     P   C Y + YAD  +S G  +RD++ L  V G   T  
Sbjct: 133 VGCDDDFCSFI---SQSDSCQPALGCSYHIVYADESTSDGKFIRDMLTLEQVTGDLKTGP 192

Query: 219 LG----FGCGYEQRNGGLQPSSSIAGVLGLGRSEASIVAQLSALGLVSNIIGHCLSGQGG 278
           LG    FGCG +Q        S++ GV+G G+S  S+++QL+A G    +  HCL    G
Sbjct: 193 LGQEVVFGCGSDQSGQLGNGDSAVDGVMGFGQSNTSVLSQLAATGDAKRVFSHCLDNVKG 252

Query: 279 GFLFFGGDLFPSSKMSWTPILRTPGGNYSVGPAEVYYGGKAVGS--EGLTLTF------- 338
           G +F  G +  S K+  TP++          P +++Y    +G   +G +L         
Sbjct: 253 GGIFAVG-VVDSPKVKTTPMV----------PNQMHYNVMLMGMDVDGTSLDLPRSIVRN 312

Query: 339 -----DSGSSYTYFNSKVYGA-VEKLLKNDLKGKLRDEPEDETLPVCWKGTKAFKSVADV 398
                DSG++  YF   +Y + +E +L            +   L +  +  + F    +V
Sbjct: 313 GGTIVDSGTTLAYFPKVLYDSLIETILAR----------QPVKLHIVEETFQCFSFSTNV 372

Query: 399 RNFFKPLALGFTNSKNQFQIPPEAYLIISKFGNVCLGISNGSQVGLGNDN-----LIGDI 440
              F P++  F +S  +  + P  YL   +    C G   G   GL  D      L+GD+
Sbjct: 373 DEAFPPVSFEFEDSV-KLTVYPHDYLFTLEEELYCFGWQAG---GLTTDERSEVILLGDL 427

BLAST of MC11g0709 vs. NCBI nr
Match: XP_022141028.1 (aspartic proteinase Asp1-like isoform X1 [Momordica charantia])

HSP 1 Score: 978 bits (2529), Expect = 0.0
Identity = 473/473 (100.00%), Postives = 473/473 (100.00%), Query Frame = 0

Query: 1   CSELHHFTPPVKPHFLGTDSKRIEKRKSSSVSGHRMRQLLLLIVSVLFANFAVSLSDKFS 60
           CSELHHFTPPVKPHFLGTDSKRIEKRKSSSVSGHRMRQLLLLIVSVLFANFAVSLSDKFS
Sbjct: 16  CSELHHFTPPVKPHFLGTDSKRIEKRKSSSVSGHRMRQLLLLIVSVLFANFAVSLSDKFS 75

Query: 61  VANWGQLEADRFGASVLFSVQGNVYPLGYFTVSINIGNPPKIFELDIDTGSDLTWVQCDA 120
           VANWGQLEADRFGASVLFSVQGNVYPLGYFTVSINIGNPPKIFELDIDTGSDLTWVQCDA
Sbjct: 76  VANWGQLEADRFGASVLFSVQGNVYPLGYFTVSINIGNPPKIFELDIDTGSDLTWVQCDA 135

Query: 121 PCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSPGQVPCNNPNDQCDYEVEYADHGSSTGV 180
           PCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSPGQVPCNNPNDQCDYEVEYADHGSSTGV
Sbjct: 136 PCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSPGQVPCNNPNDQCDYEVEYADHGSSTGV 195

Query: 181 LVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGLQPSSSIAGVLGLGRSEASIVAQLSALG 240
           LVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGLQPSSSIAGVLGLGRSEASIVAQLSALG
Sbjct: 196 LVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGLQPSSSIAGVLGLGRSEASIVAQLSALG 255

Query: 241 LVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWTPILRTPGGNYSVGPAEVYYGGKAVGSE 300
           LVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWTPILRTPGGNYSVGPAEVYYGGKAVGSE
Sbjct: 256 LVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWTPILRTPGGNYSVGPAEVYYGGKAVGSE 315

Query: 301 GLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKGKLRDEPEDETLPVCWKGTKAFKSVADV 360
           GLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKGKLRDEPEDETLPVCWKGTKAFKSVADV
Sbjct: 316 GLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKGKLRDEPEDETLPVCWKGTKAFKSVADV 375

Query: 361 RNFFKPLALGFTNSKNQFQIPPEAYLIISKFGNVCLGISNGSQVGLGNDNLIGDISMLDK 420
           RNFFKPLALGFTNSKNQFQIPPEAYLIISKFGNVCLGISNGSQVGLGNDNLIGDISMLDK
Sbjct: 376 RNFFKPLALGFTNSKNQFQIPPEAYLIISKFGNVCLGISNGSQVGLGNDNLIGDISMLDK 435

Query: 421 IVIYDNESQRIGWAPANCNKRPMKHGEQWLAGEFYPTQEKINIMTRERYTYNM 473
           IVIYDNESQRIGWAPANCNKRPMKHGEQWLAGEFYPTQEKINIMTRERYTYNM
Sbjct: 436 IVIYDNESQRIGWAPANCNKRPMKHGEQWLAGEFYPTQEKINIMTRERYTYNM 488

BLAST of MC11g0709 vs. NCBI nr
Match: XP_022141111.1 (aspartic proteinase Asp1-like isoform X2 [Momordica charantia])

HSP 1 Score: 904 bits (2336), Expect = 0.0
Identity = 438/438 (100.00%), Postives = 438/438 (100.00%), Query Frame = 0

Query: 36  MRQLLLLIVSVLFANFAVSLSDKFSVANWGQLEADRFGASVLFSVQGNVYPLGYFTVSIN 95
           MRQLLLLIVSVLFANFAVSLSDKFSVANWGQLEADRFGASVLFSVQGNVYPLGYFTVSIN
Sbjct: 1   MRQLLLLIVSVLFANFAVSLSDKFSVANWGQLEADRFGASVLFSVQGNVYPLGYFTVSIN 60

Query: 96  IGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSPGQ 155
           IGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSPGQ
Sbjct: 61  IGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSPGQ 120

Query: 156 VPCNNPNDQCDYEVEYADHGSSTGVLVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGLQP 215
           VPCNNPNDQCDYEVEYADHGSSTGVLVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGLQP
Sbjct: 121 VPCNNPNDQCDYEVEYADHGSSTGVLVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGLQP 180

Query: 216 SSSIAGVLGLGRSEASIVAQLSALGLVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWTPI 275
           SSSIAGVLGLGRSEASIVAQLSALGLVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWTPI
Sbjct: 181 SSSIAGVLGLGRSEASIVAQLSALGLVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWTPI 240

Query: 276 LRTPGGNYSVGPAEVYYGGKAVGSEGLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKGKL 335
           LRTPGGNYSVGPAEVYYGGKAVGSEGLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKGKL
Sbjct: 241 LRTPGGNYSVGPAEVYYGGKAVGSEGLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKGKL 300

Query: 336 RDEPEDETLPVCWKGTKAFKSVADVRNFFKPLALGFTNSKNQFQIPPEAYLIISKFGNVC 395
           RDEPEDETLPVCWKGTKAFKSVADVRNFFKPLALGFTNSKNQFQIPPEAYLIISKFGNVC
Sbjct: 301 RDEPEDETLPVCWKGTKAFKSVADVRNFFKPLALGFTNSKNQFQIPPEAYLIISKFGNVC 360

Query: 396 LGISNGSQVGLGNDNLIGDISMLDKIVIYDNESQRIGWAPANCNKRPMKHGEQWLAGEFY 455
           LGISNGSQVGLGNDNLIGDISMLDKIVIYDNESQRIGWAPANCNKRPMKHGEQWLAGEFY
Sbjct: 361 LGISNGSQVGLGNDNLIGDISMLDKIVIYDNESQRIGWAPANCNKRPMKHGEQWLAGEFY 420

Query: 456 PTQEKINIMTRERYTYNM 473
           PTQEKINIMTRERYTYNM
Sbjct: 421 PTQEKINIMTRERYTYNM 438

BLAST of MC11g0709 vs. NCBI nr
Match: XP_022132194.1 (aspartic proteinase Asp1-like isoform X4 [Momordica charantia])

HSP 1 Score: 645 bits (1663), Expect = 2.36e-229
Identity = 314/413 (76.03%), Postives = 352/413 (85.23%), Query Frame = 0

Query: 36  MRQLLLLIVSVLFANFAVSLSDKFSVANWGQLEA--DRFGASVLFSVQGNVYPLGYFTVS 95
           M   LL  VS+LFA FAVS SDKFS+ +  Q  +  +RF +SV F ++GNVYPLG+FTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 96  INIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSP 155
           +NIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPH+NAVRC EPLC+AL  P
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120

Query: 156 GQVPCNNPNDQCDYEVEYADHGSSTGVLVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGL 215
           G+VPC NPNDQCDY+VEYAD GSS GVLVRDL P+R  NG+   PNLGFGCGY+Q++GG 
Sbjct: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGG- 180

Query: 216 QPSSSIAGVLGLGRSEASIVAQLSALGLVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWT 275
               S AGVLGLG  +A++ +QLSALG V N++GHCLSGQGGGFLFFGGDL PSS MSWT
Sbjct: 181 ----STAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWT 240

Query: 276 PILRTPGGNYSVGPAEVYYGGKAVGSEGLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKG 335
           PI+ +PGG YS GPAEVY+GGKA G  GLTLTFDSGSSYTYFNS+VYGAV  LLKNDLKG
Sbjct: 241 PIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKG 300

Query: 336 K-LRDEPEDETLPVCWKGTKAFKSVADVRNFFKPLALGFTNSKN-QFQIPPEAYLIISKF 395
           K L DEP+D+TLPVCWKGTKAF+SV DVRNFFKPLAL FT+SKN QFQIPPEAYLIISKF
Sbjct: 301 KPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKF 360

Query: 396 GNVCLGISNGSQVGLGNDNLIGDISMLDKIVIYDNESQRIGWAPANCNKRPMK 444
           GNVC GI NGSQVGLGN N+IGDIS+LDKIV+YDNE Q+IGWAPANCN+ P K
Sbjct: 361 GNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK 408

BLAST of MC11g0709 vs. NCBI nr
Match: XP_022132187.1 (aspartic proteinase Asp1-like isoform X3 [Momordica charantia])

HSP 1 Score: 630 bits (1624), Expect = 5.63e-223
Identity = 314/441 (71.20%), Postives = 352/441 (79.82%), Query Frame = 0

Query: 36  MRQLLLLIVSVLFANFAVSLSDKFSVANWGQLEA--DRFGASVLFSVQGNVYPLGYFTVS 95
           M   LL  VS+LFA FAVS SDKFS+ +  Q  +  +RF +SV F ++GNVYPLG+FTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 96  INIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL-------------------------- 155
           +NIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL                          
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLVSLIQIDGLFDSCFLFFYRNFVPSMT 120

Query: 156 --PRDKQYRPHNNAVRCGEPLCAALVSPGQVPCNNPNDQCDYEVEYADHGSSTGVLVRDL 215
             PRDKQYRPH+NAVRC EPLC+AL  PG+VPC NPNDQCDY+VEYAD GSS GVLVRDL
Sbjct: 121 LQPRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180

Query: 216 VPLRFVNGAFLTPNLGFGCGYEQRNGGLQPSSSIAGVLGLGRSEASIVAQLSALGLVSNI 275
            P+R  NG+   PNLGFGCGY+Q++GG     S AGVLGLG  +A++ +QLSALG V N+
Sbjct: 181 FPMRLTNGSVFAPNLGFGCGYDQKHGG-----STAGVLGLGSGKATMTSQLSALGHVRNV 240

Query: 276 IGHCLSGQGGGFLFFGGDLFPSSKMSWTPILRTPGGNYSVGPAEVYYGGKAVGSEGLTLT 335
           +GHCLSGQGGGFLFFGGDL PSS MSWTPI+ +PGG YS GPAEVY+GGKA G  GLTLT
Sbjct: 241 VGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLT 300

Query: 336 FDSGSSYTYFNSKVYGAVEKLLKNDLKGK-LRDEPEDETLPVCWKGTKAFKSVADVRNFF 395
           FDSGSSYTYFNS+VYGAV  LLKNDLKGK L DEP+D+TLPVCWKGTKAF+SV DVRNFF
Sbjct: 301 FDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFF 360

Query: 396 KPLALGFTNSKN-QFQIPPEAYLIISKFGNVCLGISNGSQVGLGNDNLIGDISMLDKIVI 444
           KPLAL FT+SKN QFQIPPEAYLIISKFGNVC GI NGSQVGLGN N+IGDIS+LDKIV+
Sbjct: 361 KPLALSFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGDISLLDKIVV 420

BLAST of MC11g0709 vs. NCBI nr
Match: XP_022132180.1 (aspartic proteinase Asp1-like isoform X2 [Momordica charantia])

HSP 1 Score: 627 bits (1616), Expect = 1.24e-221
Identity = 314/449 (69.93%), Postives = 352/449 (78.40%), Query Frame = 0

Query: 36  MRQLLLLIVSVLFANFAVSLSDKFSVANWGQLEA--DRFGASVLFSVQGNVYPLGYFTVS 95
           M   LL  VS+LFA FAVS SDKFS+ +  Q  +  +RF +SV F ++GNVYPLG+FTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 96  INIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSP 155
           +NIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPH+NAVRC EPLC+AL  P
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120

Query: 156 GQVPCNNPNDQCDYEVEYADHGSSTGVLVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGL 215
           G+VPC NPNDQCDY+VEYAD GSS GVLVRDL P+R  NG+   PNLGFGCGY+Q++GG 
Sbjct: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGG- 180

Query: 216 QPSSSIAGVLGLGRSEASIVAQLSALGLVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWT 275
               S AGVLGLG  +A++ +QLSALG V N++GHCLSGQGGGFLFFGGDL PSS MSWT
Sbjct: 181 ----STAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWT 240

Query: 276 PILRTPGGNYSVGPAEVYYGGKAVGSEGLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKG 335
           PI+ +PGG YS GPAEVY+GGKA G  GLTLTFDSGSSYTYFNS+VYGAV  LLKNDLKG
Sbjct: 241 PIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKG 300

Query: 336 K-LRDEPEDETLPVCWKGTKAFKSVADVRNFFKPLALGFTNSKN-QFQIPPEAYLIISKF 395
           K L DEP+D+TLPVCWKGTKAF+SV DVRNFFKPLAL FT+SKN QFQIPPEAYLIISKF
Sbjct: 301 KPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKF 360

Query: 396 GNVCLGISNGSQVGLGNDNLIG------------------------------------DI 444
           GNVC GI NGSQVGLGN N+IG                                    DI
Sbjct: 361 GNVCFGILNGSQVGLGNVNVIGGKMRNTVGSTKNHFQVLFFYMLSSNISTSFSPKFVTDI 420

BLAST of MC11g0709 vs. ExPASy TrEMBL
Match: A0A6J1CJC7 (aspartic proteinase Asp1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011507 PE=3 SV=1)

HSP 1 Score: 978 bits (2529), Expect = 0.0
Identity = 473/473 (100.00%), Postives = 473/473 (100.00%), Query Frame = 0

Query: 1   CSELHHFTPPVKPHFLGTDSKRIEKRKSSSVSGHRMRQLLLLIVSVLFANFAVSLSDKFS 60
           CSELHHFTPPVKPHFLGTDSKRIEKRKSSSVSGHRMRQLLLLIVSVLFANFAVSLSDKFS
Sbjct: 16  CSELHHFTPPVKPHFLGTDSKRIEKRKSSSVSGHRMRQLLLLIVSVLFANFAVSLSDKFS 75

Query: 61  VANWGQLEADRFGASVLFSVQGNVYPLGYFTVSINIGNPPKIFELDIDTGSDLTWVQCDA 120
           VANWGQLEADRFGASVLFSVQGNVYPLGYFTVSINIGNPPKIFELDIDTGSDLTWVQCDA
Sbjct: 76  VANWGQLEADRFGASVLFSVQGNVYPLGYFTVSINIGNPPKIFELDIDTGSDLTWVQCDA 135

Query: 121 PCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSPGQVPCNNPNDQCDYEVEYADHGSSTGV 180
           PCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSPGQVPCNNPNDQCDYEVEYADHGSSTGV
Sbjct: 136 PCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSPGQVPCNNPNDQCDYEVEYADHGSSTGV 195

Query: 181 LVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGLQPSSSIAGVLGLGRSEASIVAQLSALG 240
           LVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGLQPSSSIAGVLGLGRSEASIVAQLSALG
Sbjct: 196 LVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGLQPSSSIAGVLGLGRSEASIVAQLSALG 255

Query: 241 LVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWTPILRTPGGNYSVGPAEVYYGGKAVGSE 300
           LVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWTPILRTPGGNYSVGPAEVYYGGKAVGSE
Sbjct: 256 LVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWTPILRTPGGNYSVGPAEVYYGGKAVGSE 315

Query: 301 GLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKGKLRDEPEDETLPVCWKGTKAFKSVADV 360
           GLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKGKLRDEPEDETLPVCWKGTKAFKSVADV
Sbjct: 316 GLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKGKLRDEPEDETLPVCWKGTKAFKSVADV 375

Query: 361 RNFFKPLALGFTNSKNQFQIPPEAYLIISKFGNVCLGISNGSQVGLGNDNLIGDISMLDK 420
           RNFFKPLALGFTNSKNQFQIPPEAYLIISKFGNVCLGISNGSQVGLGNDNLIGDISMLDK
Sbjct: 376 RNFFKPLALGFTNSKNQFQIPPEAYLIISKFGNVCLGISNGSQVGLGNDNLIGDISMLDK 435

Query: 421 IVIYDNESQRIGWAPANCNKRPMKHGEQWLAGEFYPTQEKINIMTRERYTYNM 473
           IVIYDNESQRIGWAPANCNKRPMKHGEQWLAGEFYPTQEKINIMTRERYTYNM
Sbjct: 436 IVIYDNESQRIGWAPANCNKRPMKHGEQWLAGEFYPTQEKINIMTRERYTYNM 488

BLAST of MC11g0709 vs. ExPASy TrEMBL
Match: A0A6J1CI16 (aspartic proteinase Asp1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011507 PE=3 SV=1)

HSP 1 Score: 904 bits (2336), Expect = 0.0
Identity = 438/438 (100.00%), Postives = 438/438 (100.00%), Query Frame = 0

Query: 36  MRQLLLLIVSVLFANFAVSLSDKFSVANWGQLEADRFGASVLFSVQGNVYPLGYFTVSIN 95
           MRQLLLLIVSVLFANFAVSLSDKFSVANWGQLEADRFGASVLFSVQGNVYPLGYFTVSIN
Sbjct: 1   MRQLLLLIVSVLFANFAVSLSDKFSVANWGQLEADRFGASVLFSVQGNVYPLGYFTVSIN 60

Query: 96  IGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSPGQ 155
           IGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSPGQ
Sbjct: 61  IGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSPGQ 120

Query: 156 VPCNNPNDQCDYEVEYADHGSSTGVLVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGLQP 215
           VPCNNPNDQCDYEVEYADHGSSTGVLVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGLQP
Sbjct: 121 VPCNNPNDQCDYEVEYADHGSSTGVLVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGLQP 180

Query: 216 SSSIAGVLGLGRSEASIVAQLSALGLVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWTPI 275
           SSSIAGVLGLGRSEASIVAQLSALGLVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWTPI
Sbjct: 181 SSSIAGVLGLGRSEASIVAQLSALGLVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWTPI 240

Query: 276 LRTPGGNYSVGPAEVYYGGKAVGSEGLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKGKL 335
           LRTPGGNYSVGPAEVYYGGKAVGSEGLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKGKL
Sbjct: 241 LRTPGGNYSVGPAEVYYGGKAVGSEGLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKGKL 300

Query: 336 RDEPEDETLPVCWKGTKAFKSVADVRNFFKPLALGFTNSKNQFQIPPEAYLIISKFGNVC 395
           RDEPEDETLPVCWKGTKAFKSVADVRNFFKPLALGFTNSKNQFQIPPEAYLIISKFGNVC
Sbjct: 301 RDEPEDETLPVCWKGTKAFKSVADVRNFFKPLALGFTNSKNQFQIPPEAYLIISKFGNVC 360

Query: 396 LGISNGSQVGLGNDNLIGDISMLDKIVIYDNESQRIGWAPANCNKRPMKHGEQWLAGEFY 455
           LGISNGSQVGLGNDNLIGDISMLDKIVIYDNESQRIGWAPANCNKRPMKHGEQWLAGEFY
Sbjct: 361 LGISNGSQVGLGNDNLIGDISMLDKIVIYDNESQRIGWAPANCNKRPMKHGEQWLAGEFY 420

Query: 456 PTQEKINIMTRERYTYNM 473
           PTQEKINIMTRERYTYNM
Sbjct: 421 PTQEKINIMTRERYTYNM 438

BLAST of MC11g0709 vs. ExPASy TrEMBL
Match: A0A6J1BSD8 (aspartic proteinase Asp1-like isoform X4 OS=Momordica charantia OX=3673 GN=LOC111005085 PE=3 SV=1)

HSP 1 Score: 645 bits (1663), Expect = 1.14e-229
Identity = 314/413 (76.03%), Postives = 352/413 (85.23%), Query Frame = 0

Query: 36  MRQLLLLIVSVLFANFAVSLSDKFSVANWGQLEA--DRFGASVLFSVQGNVYPLGYFTVS 95
           M   LL  VS+LFA FAVS SDKFS+ +  Q  +  +RF +SV F ++GNVYPLG+FTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 96  INIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSP 155
           +NIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPH+NAVRC EPLC+AL  P
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120

Query: 156 GQVPCNNPNDQCDYEVEYADHGSSTGVLVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGL 215
           G+VPC NPNDQCDY+VEYAD GSS GVLVRDL P+R  NG+   PNLGFGCGY+Q++GG 
Sbjct: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGG- 180

Query: 216 QPSSSIAGVLGLGRSEASIVAQLSALGLVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWT 275
               S AGVLGLG  +A++ +QLSALG V N++GHCLSGQGGGFLFFGGDL PSS MSWT
Sbjct: 181 ----STAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWT 240

Query: 276 PILRTPGGNYSVGPAEVYYGGKAVGSEGLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKG 335
           PI+ +PGG YS GPAEVY+GGKA G  GLTLTFDSGSSYTYFNS+VYGAV  LLKNDLKG
Sbjct: 241 PIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKG 300

Query: 336 K-LRDEPEDETLPVCWKGTKAFKSVADVRNFFKPLALGFTNSKN-QFQIPPEAYLIISKF 395
           K L DEP+D+TLPVCWKGTKAF+SV DVRNFFKPLAL FT+SKN QFQIPPEAYLIISKF
Sbjct: 301 KPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKF 360

Query: 396 GNVCLGISNGSQVGLGNDNLIGDISMLDKIVIYDNESQRIGWAPANCNKRPMK 444
           GNVC GI NGSQVGLGN N+IGDIS+LDKIV+YDNE Q+IGWAPANCN+ P K
Sbjct: 361 GNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK 408

BLAST of MC11g0709 vs. ExPASy TrEMBL
Match: A0A6J1BT55 (aspartic proteinase Asp1-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111005085 PE=3 SV=1)

HSP 1 Score: 630 bits (1624), Expect = 2.73e-223
Identity = 314/441 (71.20%), Postives = 352/441 (79.82%), Query Frame = 0

Query: 36  MRQLLLLIVSVLFANFAVSLSDKFSVANWGQLEA--DRFGASVLFSVQGNVYPLGYFTVS 95
           M   LL  VS+LFA FAVS SDKFS+ +  Q  +  +RF +SV F ++GNVYPLG+FTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 96  INIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL-------------------------- 155
           +NIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL                          
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLVSLIQIDGLFDSCFLFFYRNFVPSMT 120

Query: 156 --PRDKQYRPHNNAVRCGEPLCAALVSPGQVPCNNPNDQCDYEVEYADHGSSTGVLVRDL 215
             PRDKQYRPH+NAVRC EPLC+AL  PG+VPC NPNDQCDY+VEYAD GSS GVLVRDL
Sbjct: 121 LQPRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180

Query: 216 VPLRFVNGAFLTPNLGFGCGYEQRNGGLQPSSSIAGVLGLGRSEASIVAQLSALGLVSNI 275
            P+R  NG+   PNLGFGCGY+Q++GG     S AGVLGLG  +A++ +QLSALG V N+
Sbjct: 181 FPMRLTNGSVFAPNLGFGCGYDQKHGG-----STAGVLGLGSGKATMTSQLSALGHVRNV 240

Query: 276 IGHCLSGQGGGFLFFGGDLFPSSKMSWTPILRTPGGNYSVGPAEVYYGGKAVGSEGLTLT 335
           +GHCLSGQGGGFLFFGGDL PSS MSWTPI+ +PGG YS GPAEVY+GGKA G  GLTLT
Sbjct: 241 VGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLT 300

Query: 336 FDSGSSYTYFNSKVYGAVEKLLKNDLKGK-LRDEPEDETLPVCWKGTKAFKSVADVRNFF 395
           FDSGSSYTYFNS+VYGAV  LLKNDLKGK L DEP+D+TLPVCWKGTKAF+SV DVRNFF
Sbjct: 301 FDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFF 360

Query: 396 KPLALGFTNSKN-QFQIPPEAYLIISKFGNVCLGISNGSQVGLGNDNLIGDISMLDKIVI 444
           KPLAL FT+SKN QFQIPPEAYLIISKFGNVC GI NGSQVGLGN N+IGDIS+LDKIV+
Sbjct: 361 KPLALSFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGDISLLDKIVV 420

BLAST of MC11g0709 vs. ExPASy TrEMBL
Match: A0A6J1BRQ3 (aspartic proteinase Asp1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005085 PE=3 SV=1)

HSP 1 Score: 627 bits (1616), Expect = 6.01e-222
Identity = 314/449 (69.93%), Postives = 352/449 (78.40%), Query Frame = 0

Query: 36  MRQLLLLIVSVLFANFAVSLSDKFSVANWGQLEA--DRFGASVLFSVQGNVYPLGYFTVS 95
           M   LL  VS+LFA FAVS SDKFS+ +  Q  +  +RF +SV F ++GNVYPLG+FTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 96  INIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSP 155
           +NIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPH+NAVRC EPLC+AL  P
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120

Query: 156 GQVPCNNPNDQCDYEVEYADHGSSTGVLVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGL 215
           G+VPC NPNDQCDY+VEYAD GSS GVLVRDL P+R  NG+   PNLGFGCGY+Q++GG 
Sbjct: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGG- 180

Query: 216 QPSSSIAGVLGLGRSEASIVAQLSALGLVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWT 275
               S AGVLGLG  +A++ +QLSALG V N++GHCLSGQGGGFLFFGGDL PSS MSWT
Sbjct: 181 ----STAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWT 240

Query: 276 PILRTPGGNYSVGPAEVYYGGKAVGSEGLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKG 335
           PI+ +PGG YS GPAEVY+GGKA G  GLTLTFDSGSSYTYFNS+VYGAV  LLKNDLKG
Sbjct: 241 PIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKG 300

Query: 336 K-LRDEPEDETLPVCWKGTKAFKSVADVRNFFKPLALGFTNSKN-QFQIPPEAYLIISKF 395
           K L DEP+D+TLPVCWKGTKAF+SV DVRNFFKPLAL FT+SKN QFQIPPEAYLIISKF
Sbjct: 301 KPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKF 360

Query: 396 GNVCLGISNGSQVGLGNDNLIG------------------------------------DI 444
           GNVC GI NGSQVGLGN N+IG                                    DI
Sbjct: 361 GNVCFGILNGSQVGLGNVNVIGGKMRNTVGSTKNHFQVLFFYMLSSNISTSFSPKFVTDI 420

BLAST of MC11g0709 vs. TAIR 10
Match: AT1G44130.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 450.7 bits (1158), Expect = 1.5e-126
Identity = 206/372 (55.38%), Postives = 269/372 (72.31%), Query Frame = 0

Query: 74  ASVLFSVQGNVYPLGYFTVSINIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQY 133
           +SV+F + GNV+PLGY++V + IG+PPK F+ DIDTGSDLTWVQCDAPC+GCTLP + QY
Sbjct: 33  SSVVFPLSGNVFPLGYYSVLMQIGSPPKAFQFDIDTGSDLTWVQCDAPCSGCTLPPNLQY 92

Query: 134 RPHNNAVRCGEPLCAALVSPGQVPCNNPNDQCDYEVEYADHGSSTGVLVRDLVPLRFVNG 193
           +P  N + C  P+C AL  P +  C NP +QCDYEV+YAD GSS G LV D  PL+ VNG
Sbjct: 93  KPKGNIIPCSNPICTALHWPNKPHCPNPQEQCDYEVKYADQGSSMGALVTDQFPLKLVNG 152

Query: 194 AFLTPNLGFGCGYEQRNGGLQPSSSIAGVLGLGRSEASIVAQLSALGLVSNIIGHCLSGQ 253
           +F+ P + FGCGY+Q      P  + AGVLGLGR +  ++ QL + GL  N++GHCLS +
Sbjct: 153 SFMQPPVAFGCGYDQSYPSAHPPPATAGVLGLGRGKIGLLTQLVSAGLTRNVVGHCLSSK 212

Query: 254 GGGFLFFGGDLFPSSKMSWTPILRTPGGNYSVGPAEVYYGGKAVGSEGLTLTFDSGSSYT 313
           GGGFLFFG +L PS  ++WTP+L +   +Y+ GPA++ + GK  G +GL L FD+GSSYT
Sbjct: 213 GGGFLFFGDNLVPSIGVAWTPLL-SQDNHYTTGPADLLFNGKPTGLKGLKLIFDTGSSYT 272

Query: 314 YFNSKVYGAVEKLLKNDLK-GKLRDEPEDETLPVCWKGTKAFKSVADVRNFFKPLALGFT 373
           YFNSK Y  +  L+ NDLK   L+   ED+TLP+CWKG K FKSV +V+NFFK + + FT
Sbjct: 273 YFNSKAYQTIINLIGNDLKVSPLKVAKEDKTLPICWKGAKPFKSVLEVKNFFKTITINFT 332

Query: 374 NSK--NQFQIPPEAYLIISKFGNVCLGISNGSQVGLGNDNLIGDISMLDKIVIYDNESQR 433
           N +   Q  + PE YLI+SK GNVCLG+ NGS+VGL N N+IGDISM   ++IYDNE Q+
Sbjct: 333 NGRRNTQLYLAPELYLIVSKTGNVCLGLLNGSEVGLQNSNVIGDISMQGLMMIYDNEKQQ 392

Query: 434 IGWAPANCNKRP 443
           +GW  ++CNK P
Sbjct: 393 LGWVSSDCNKLP 403

BLAST of MC11g0709 vs. TAIR 10
Match: AT1G77480.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 442.2 bits (1136), Expect = 5.3e-124
Identity = 216/424 (50.94%), Postives = 286/424 (67.45%), Query Frame = 0

Query: 39  LLLLIVSVLFANFAVSLSDKFSVANWGQLEADRFGASVLFSVQGNVYPLGYFTVSINIGN 98
           + +  V +L A F  S + K S A   +L+  R  ++V+F V GNVYPLGY+ V +NIGN
Sbjct: 17  VFVFYVFILCARFQTSEATKDSSAQ-VKLQNRRLSSTVVFPVSGNVYPLGYYYVLLNIGN 76

Query: 99  PPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSPGQVPC 158
           PPK+F+LDIDTGSDLTWVQCDAPC GCT PR KQY+P++N + C   LC+ L  P   PC
Sbjct: 77  PPKLFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQYKPNHNTLPCSHILCSGLDLPQDRPC 136

Query: 159 NNPNDQCDYEVEYADHGSSTGVLVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGLQPSSS 218
            +P DQCDYE+ Y+DH SS G LV D VPL+  NG+ +   L FGCGY+Q+N G  P   
Sbjct: 137 ADPEDQCDYEIGYSDHASSIGALVTDEVPLKLANGSIMNLRLTFGCGYDQQNPGPHPPPP 196

Query: 219 IAGVLGLGRSEASIVAQLSALGLVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWTPI-LR 278
            AG+LGLGR +  +  QL +LG+  N+I HCLS  G GFL  G +L PSS ++WT +   
Sbjct: 197 TAGILGLGRGKVGLSTQLKSLGITKNVIVHCLSHTGKGFLSIGDELVPSSGVTWTSLATN 256

Query: 279 TPGGNYSVGPAEVYYGGKAVGSEGLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKGK-LR 338
           +P  NY  GPAE+ +  K  G +G+ + FDSGSSYTYFN++ Y A+  L++ DL GK L 
Sbjct: 257 SPSKNYMAGPAELLFNDKTTGVKGINVVFDSGSSYTYFNAEAYQAILDLIRKDLNGKPLT 316

Query: 339 DEPEDETLPVCWKGTKAFKSVADVRNFFKPLALGFTNSKN--QFQIPPEAYLIISKFGNV 398
           D  +D++LPVCWKG K  KS+ +V+ +FK + L F N KN   FQ+PPE+YLII++ G V
Sbjct: 317 DTKDDKSLPVCWKGKKPLKSLDEVKKYFKTITLRFGNQKNGQLFQVPPESYLIITEKGRV 376

Query: 399 CLGISNGSQVGLGNDNLIGDISMLDKIVIYDNESQRIGWAPANCNKRPMKHGEQW--LAG 457
           CLGI NG+++GL   N+IGDIS    +VIYDNE QRIGW  ++C+K P  + +    L+ 
Sbjct: 377 CLGILNGTEIGLEGYNIIGDISFQGIMVIYDNEKQRIGWISSDCDKLPNVNHDYGGDLSE 436

BLAST of MC11g0709 vs. TAIR 10
Match: AT1G77480.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 441.4 bits (1134), Expect = 9.0e-124
Identity = 212/408 (51.96%), Postives = 279/408 (68.38%), Query Frame = 0

Query: 39  LLLLIVSVLFANFAVSLSDKFSVANWGQLEADRFGASVLFSVQGNVYPLGYFTVSINIGN 98
           + +  V +L A F  S + K S A   +L+  R  ++V+F V GNVYPLGY+ V +NIGN
Sbjct: 17  VFVFYVFILCARFQTSEATKDSSAQ-VKLQNRRLSSTVVFPVSGNVYPLGYYYVLLNIGN 76

Query: 99  PPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSPGQVPC 158
           PPK+F+LDIDTGSDLTWVQCDAPC GCT PR KQY+P++N + C   LC+ L  P   PC
Sbjct: 77  PPKLFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQYKPNHNTLPCSHILCSGLDLPQDRPC 136

Query: 159 NNPNDQCDYEVEYADHGSSTGVLVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGLQPSSS 218
            +P DQCDYE+ Y+DH SS G LV D VPL+  NG+ +   L FGCGY+Q+N G  P   
Sbjct: 137 ADPEDQCDYEIGYSDHASSIGALVTDEVPLKLANGSIMNLRLTFGCGYDQQNPGPHPPPP 196

Query: 219 IAGVLGLGRSEASIVAQLSALGLVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWTPI-LR 278
            AG+LGLGR +  +  QL +LG+  N+I HCLS  G GFL  G +L PSS ++WT +   
Sbjct: 197 TAGILGLGRGKVGLSTQLKSLGITKNVIVHCLSHTGKGFLSIGDELVPSSGVTWTSLATN 256

Query: 279 TPGGNYSVGPAEVYYGGKAVGSEGLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKGK-LR 338
           +P  NY  GPAE+ +  K  G +G+ + FDSGSSYTYFN++ Y A+  L++ DL GK L 
Sbjct: 257 SPSKNYMAGPAELLFNDKTTGVKGINVVFDSGSSYTYFNAEAYQAILDLIRKDLNGKPLT 316

Query: 339 DEPEDETLPVCWKGTKAFKSVADVRNFFKPLALGFTNSKN--QFQIPPEAYLIISKFGNV 398
           D  +D++LPVCWKG K  KS+ +V+ +FK + L F N KN   FQ+PPE+YLII++ G V
Sbjct: 317 DTKDDKSLPVCWKGKKPLKSLDEVKKYFKTITLRFGNQKNGQLFQVPPESYLIITEKGRV 376

Query: 399 CLGISNGSQVGLGNDNLIGDISMLDKIVIYDNESQRIGWAPANCNKRP 443
           CLGI NG+++GL   N+IGDIS    +VIYDNE QRIGW  ++C+K P
Sbjct: 377 CLGILNGTEIGLEGYNIIGDISFQGIMVIYDNEKQRIGWISSDCDKLP 423

BLAST of MC11g0709 vs. TAIR 10
Match: AT4G33490.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 417.2 bits (1071), Expect = 1.8e-116
Identity = 210/410 (51.22%), Postives = 274/410 (66.83%), Query Frame = 0

Query: 36  MRQLLLLIVSVLFANFAVSLSDKFSVANWGQLEADRFGASVLFSVQGNVYPLGYFTVSIN 95
           +R +++L+V  L   F+ ++  ++           R  +SV+F V GNVYPLGY+ V+IN
Sbjct: 6   VRFMIVLMVMSLVLGFSSAVDFRWRKTAGFSDRFTRAVSSVVFPVHGNVYPLGYYNVTIN 65

Query: 96  IGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSPGQ 155
           IG PP+ + LD+DTGSDLTW+QCDAPC  C       Y+P ++ + C +PLC AL     
Sbjct: 66  IGQPPRPYYLDLDTGSDLTWLQCDAPCVRCLEAPHPLYQPSSDLIPCNDPLCKALHLNSN 125

Query: 156 VPCNNPNDQCDYEVEYADHGSSTGVLVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGLQP 215
             C  P +QCDYEVEYAD GSS GVLVRD+  + +  G  LTP L  GCGY+Q  G    
Sbjct: 126 QRCETP-EQCDYEVEYADGGSSLGVLVRDVFSMNYTQGLRLTPRLALGCGYDQIPGA-SS 185

Query: 216 SSSIAGVLGLGRSEASIVAQLSALGLVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWTPI 275
              + GVLGLGR + SI++QL + G V N+IGHCLS  GGG LFFG DL+ SS++SWTP+
Sbjct: 186 HHPLDGVLGLGRGKVSILSQLHSQGYVKNVIGHCLSSLGGGILFFGDDLYDSSRVSWTPM 245

Query: 276 LRTPGGNYSVG-PAEVYYGGKAVGSEGLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKGK 335
            R    +YS     E+ +GG+  G + L   FDSGSSYTYFNSK Y AV  LLK +L GK
Sbjct: 246 SREYSKHYSPAMGGELLFGGRTTGLKNLLTVFDSGSSYTYFNSKAYQAVTYLLKRELSGK 305

Query: 336 -LRDEPEDETLPVCWKGTKAFKSVADVRNFFKPLALGFT---NSKNQFQIPPEAYLIISK 395
            L++  +D TLP+CW+G + F S+ +V+ +FKPLAL F     SK  F+IPPEAYLIIS 
Sbjct: 306 PLKEARDDHTLPLCWQGRRPFMSIEEVKKYFKPLALSFKTGWRSKTLFEIPPEAYLIISM 365

Query: 396 FGNVCLGISNGSQVGLGNDNLIGDISMLDKIVIYDNESQRIGWAPANCNK 441
            GNVCLGI NG+++GL N NLIGDISM D+++IYDNE Q IGW P +C++
Sbjct: 366 KGNVCLGILNGTEIGLQNLNLIGDISMQDQMIIYDNEKQSIGWMPVDCDE 413

BLAST of MC11g0709 vs. TAIR 10
Match: AT4G33490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 377.9 bits (969), Expect = 1.2e-104
Identity = 194/383 (50.65%), Postives = 252/383 (65.80%), Query Frame = 0

Query: 36  MRQLLLLIVSVLFANFAVSLSDKFSVANWGQLEADRFGASVLFSVQGNVYPLGYFTVSIN 95
           +R +++L+V  L   F+ ++  ++           R  +SV+F V GNVYPLGY+ V+IN
Sbjct: 3   VRFMIVLMVMSLVLGFSSAVDFRWRKTAGFSDRFTRAVSSVVFPVHGNVYPLGYYNVTIN 62

Query: 96  IGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHNNAVRCGEPLCAALVSPGQ 155
           IG PP+ + LD+DTGSDLTW+QCDAPC  C       Y+P ++ + C +PLC AL     
Sbjct: 63  IGQPPRPYYLDLDTGSDLTWLQCDAPCVRCLEAPHPLYQPSSDLIPCNDPLCKALHLNSN 122

Query: 156 VPCNNPNDQCDYEVEYADHGSSTGVLVRDLVPLRFVNGAFLTPNLGFGCGYEQRNGGLQP 215
             C  P +QCDYEVEYAD GSS GVLVRD+  + +  G  LTP L  GCGY+Q  G    
Sbjct: 123 QRCETP-EQCDYEVEYADGGSSLGVLVRDVFSMNYTQGLRLTPRLALGCGYDQIPGA-SS 182

Query: 216 SSSIAGVLGLGRSEASIVAQLSALGLVSNIIGHCLSGQGGGFLFFGGDLFPSSKMSWTPI 275
              + GVLGLGR + SI++QL + G V N+IGHCLS  GGG LFFG DL+ SS++SWTP+
Sbjct: 183 HHPLDGVLGLGRGKVSILSQLHSQGYVKNVIGHCLSSLGGGILFFGDDLYDSSRVSWTPM 242

Query: 276 LRTPGGNYSVG-PAEVYYGGKAVGSEGLTLTFDSGSSYTYFNSKVYGAVEKLLKNDLKGK 335
            R    +YS     E+ +GG+  G + L   FDSGSSYTYFNSK Y AV  LLK +L GK
Sbjct: 243 SREYSKHYSPAMGGELLFGGRTTGLKNLLTVFDSGSSYTYFNSKAYQAVTYLLKRELSGK 302

Query: 336 -LRDEPEDETLPVCWKGTKAFKSVADVRNFFKPLALGFT---NSKNQFQIPPEAYLIISK 395
            L++  +D TLP+CW+G + F S+ +V+ +FKPLAL F     SK  F+IPPEAYLIIS 
Sbjct: 303 PLKEARDDHTLPLCWQGRRPFMSIEEVKKYFKPLALSFKTGWRSKTLFEIPPEAYLIISM 362

Query: 396 FGNVCLGISNGSQVGLGNDNLIG 414
            GNVCLGI NG+++GL N NLIG
Sbjct: 363 KGNVCLGILNGTEIGLQNLNLIG 383