MC11g0708 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g0708 vs. ExPASy Swiss-Prot
Match: Q0IU52 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. japonica OX=39947 GN=ASP1 PE=2 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 7.2e-82
Identity = 168/384 (43.75%), Postives = 238/384 (61.98%), Query Frame = 0

Query: 40  ASSVAFPIKGNVYPLGHFTVSVNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQ 99
           +S+V   + GNVYP+GHF +++NIG+P K + LDIDTGS LTW+QCDAPCT C +     
Sbjct: 21  SSAVVLELHGNVYPIGHFFITMNIGDPAKSYFLDIDTGSTLTWLQCDAPCTNCNIVPHVL 80

Query: 100 YRP-HSNAVRCAEPLCSALFFP-GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRL 159
           Y+P     V CA+ LC+ L+   GK        QCDY ++Y D  SS+GVLV D F +  
Sbjct: 81  YKPTPKKLVTCADSLCTDLYTDLGKPKRCGSQKQCDYVIQYVD-SSSMGVLVIDRFSLSA 140

Query: 160 TNGSVFAPNLGFGCGYDQKHGGSTA-----GVLGLGSGKATMTSQLSALGHV-RNVVGHC 219
           +NG+     + FGCGYDQ             +LGL  GK T+ SQL + G + ++V+GHC
Sbjct: 141 SNGT-NPTTIAFGCGYDQGKKNRNVPIPVDSILGLSRGKVTLLSQLKSQGVITKHVLGHC 200

Query: 220 LSGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYF--GGKAAGIRGLTLTFD 279
           +S +GGGFLFFG   VP+SG++WTP M      YS G   ++F    KA     + + FD
Sbjct: 201 ISSKGGGFLFFGDAQVPTSGVTWTP-MNREHKYYSPGHGTLHFDSNSKAISAAPMAVIFD 260

Query: 280 SGSSYTYFNSQVYGAVLNLLKNDLKG--KPLSD-EPKDKTLPVCWKGTKAFRSVVDVRNF 339
           SG++YTYF +Q Y A L+++K+ L    K L++   KD+ L VCWKG     ++ +V+  
Sbjct: 261 SGATYTYFAAQPYQATLSVVKSTLNSECKFLTEVTEKDRALTVCWKGKDKIVTIDEVKKC 320

Query: 340 FKPLALSFTD-SKNVQFQIPPEAYLIISKFGNVCFGILNGSQ--VGLGNVNVIGDISLLD 399
           F+ L+L F D  K    +IPPE YLIIS+ G+VC GIL+GS+  + L   N+IG I++LD
Sbjct: 321 FRSLSLEFADGDKKATLEIPPEHYLIISQEGHVCLGILDGSKEHLSLAGTNLIGGITMLD 380

Query: 400 KIVVYDNERQQIGWAPANCNRPPK 408
           ++V+YD+ER  +GW    C+R P+
Sbjct: 381 QMVIYDSERSLLGWVNYQCDRIPR 401

BLAST of MC11g0708 vs. ExPASy Swiss-Prot
Match: A2ZC67 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=2)

HSP 1 Score: 299.7 bits (766), Expect = 5.1e-80
Identity = 167/384 (43.49%), Postives = 240/384 (62.50%), Query Frame = 0

Query: 40  ASSVAFPIKGNVYPLGHFTVSVNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQ 99
           +S+V   + GNVYP+GHF V++NIG+P K + LDIDTGS LTW+QCD PC  C       
Sbjct: 21  SSAVVLELHGNVYPIGHFFVTMNIGDPAKPYFLDIDTGSTLTWLQCDYPCINCNKVPHGL 80

Query: 100 YRPH-SNAVRCAEPLCSALFFPGKVPCK-NPNDQCDYDVEYADQGSSIGVLVRDLFPMRL 159
           Y+P    AV+C E  C+ L+   + P K  P +QC Y ++Y   GSSIGVL+ D F +  
Sbjct: 81  YKPELKYAVKCTEQRCADLYADLRKPMKCGPKNQCHYGIQYVG-GSSIGVLIVDSFSLPA 140

Query: 160 TNGSVFAPNLGFGCGYDQ---KHGGSTA--GVLGLGSGKATMTSQLSALGHV-RNVVGHC 219
           +NG+    ++ FGCGY+Q    H   T   G+LGLG GK T+ SQL + G + ++V+GHC
Sbjct: 141 SNGT-NPTSIAFGCGYNQGKNNHNVPTPVNGILGLGRGKVTLLSQLKSQGVITKHVLGHC 200

Query: 220 LSGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRG--LTLTFD 279
           +S +G GFLFFG   VP+SG++W+P M      YS     + F   +  I    + + FD
Sbjct: 201 ISSKGKGFLFFGDAKVPTSGVTWSP-MNREHKHYSPRQGTLQFNSNSKPISAAPMEVIFD 260

Query: 280 SGSSYTYFNSQVYGAVLNLLKNDL--KGKPLSD-EPKDKTLPVCWKGTKAFRSVVDVRNF 339
           SG++YTYF  Q Y A L+++K+ L  + K L++ + KD+ L VCWKG    R++ +V+  
Sbjct: 261 SGATYTYFALQPYHATLSVVKSTLSKECKFLTEVKEKDRALTVCWKGKDKIRTIDEVKKC 320

Query: 340 FKPLALSFTD-SKNVQFQIPPEAYLIISKFGNVCFGILNGSQ--VGLGNVNVIGDISLLD 399
           F+ L+L F D  K    +IPPE YLIIS+ G+VC GIL+GS+    L   N+IG I++LD
Sbjct: 321 FRSLSLKFADGDKKATLEIPPEHYLIISQEGHVCLGILDGSKEHPSLAGTNLIGGITMLD 380

Query: 400 KIVVYDNERQQIGWAPANCNRPPK 408
           ++V+YD+ER  +GW    C+R P+
Sbjct: 381 QMVIYDSERSLLGWVNYQCDRIPR 401

BLAST of MC11g0708 vs. ExPASy Swiss-Prot
Match: Q9M9A8 (Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.2e-76
Identity = 162/398 (40.70%), Postives = 236/398 (59.30%), Query Frame = 0

Query: 32  SGSGSNRFASSVAFPIKGNVYPLGHFTVSVNIGNPP--KIFELDIDTGSDLTWVQCDAPC 91
           S S  +  +S+  FP+ GNVYP G +   + +G P   + + LDIDTGS+LTW+QCDAPC
Sbjct: 178 STSAGSIDSSTTIFPVGGNVYPDGLYYTRILVGKPEDGQYYHLDIDTGSELTWIQCDAPC 237

Query: 92  TGCTLPRDKQYRPH-SNAVRCAEPLCSALFFPGKVP-CKNPNDQCDYDVEYADQGSSIGV 151
           T C    ++ Y+P   N VR +E  C  +        C+N + QCDY++EYAD   S+GV
Sbjct: 238 TSCAKGANQLYKPRKDNLVRSSEAFCVEVQRNQLTEHCENCH-QCDYEIEYADHSYSMGV 297

Query: 152 LVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGG-------STAGVLGLGSGKATMTSQLSA 211
           L +D F ++L NGS+   ++ FGCGYDQ+  G        T G+LGL   K ++ SQL++
Sbjct: 298 LTKDKFHLKLHNGSLAESDIVFGCGYDQQ--GLLLNTLLKTDGILGLSRAKISLPSQLAS 357

Query: 212 LGHVRNVVGHCLSG--QGGGFLFFGGDLVPSSGMSWTPIM-PSPGGRYSSGPAEVYFGGK 271
            G + NVVGHCL+    G G++F G DLVPS GM+W P++  S    Y     ++ +G  
Sbjct: 358 RGIISNVVGHCLASDLNGEGYIFMGSDLVPSHGMTWVPMLHDSRLDAYQMQVTKMSYGQG 417

Query: 272 AAGIRGLT-----LTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCW- 331
              + G       + FD+GSSYTYF +Q Y  ++  L+ ++ G  L+ +  D+TLP+CW 
Sbjct: 418 MLSLDGENGRVGKVLFDTGSSYTYFPNQAYSQLVTSLQ-EVSGLELTRDDSDETLPICWR 477

Query: 332 -KGTKAFRSVVDVRNFFKPLALSFTDSKNV---QFQIPPEAYLIISKFGNVCFGILNGSQ 391
            K    F S+ DV+ FF+P+ L       +   +  I PE YLIIS  GNVC GIL+GS 
Sbjct: 478 AKTNFPFSSLSDVKKFFRPITLQIGSKWLIISRKLLIQPEDYLIISNKGNVCLGILDGSS 537

Query: 392 VGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRP 406
           V  G+  ++GDIS+   ++VYDN +++IGW  ++C RP
Sbjct: 538 VHDGSTIILGDISMRGHLIVYDNVKRRIGWMKSDCVRP 571

BLAST of MC11g0708 vs. ExPASy Swiss-Prot
Match: Q9S9K4 (Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2)

HSP 1 Score: 147.1 bits (370), Expect = 4.3e-34
Identity = 114/394 (28.93%), Postives = 181/394 (45.94%), Query Frame = 0

Query: 37  NRFASSVAFPIKGN--VYPLGHFTVSVNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL 96
           +R  +S+  P+ G+  V  +G +   + +G+PPK + + +DTGSD+ W+ C  PC  C  
Sbjct: 52  SRMLASIDLPLGGDSRVDSVGLYFTKIKLGSPPKEYHVQVDTGSDILWINC-KPCPKCPT 111

Query: 97  PRDKQYR---------PHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSI 156
             +  +R           S  V C +  CS  F      C+ P   C Y + YAD+ +S 
Sbjct: 112 KTNLNFRLSLFDMNASSTSKKVGCDDDFCS--FISQSDSCQ-PALGCSYHIVYADESTSD 171

Query: 157 GVLVRDLFPMRLTNGSVFAPNLG----FGCGYDQK----HGGSTA-GVLGLGSGKATMTS 216
           G  +RD+  +    G +    LG    FGCG DQ     +G S   GV+G G    ++ S
Sbjct: 172 GKFIRDMLTLEQVTGDLKTGPLGQEVVFGCGSDQSGQLGNGDSAVDGVMGFGQSNTSVLS 231

Query: 217 QLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGG 276
           QL+A G  + V  HCL    GG +F  G +V S  +  TP++P+    Y+     +   G
Sbjct: 232 QLAATGDAKRVFSHCLDNVKGGGIFAVG-VVDSPKVKTTPMVPNQ-MHYNVMLMGMDVDG 291

Query: 277 KAAG-----IRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCW 336
            +       +R      DSG++  YF   +Y    +L++  L  +P+     ++T     
Sbjct: 292 TSLDLPRSIVRNGGTIVDSGTTLAYFPKVLYD---SLIETILARQPVKLHIVEETF---- 351

Query: 337 KGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQV--G 396
              + F    +V   F P++  F DS  V+  + P  YL   +    CFG   G      
Sbjct: 352 ---QCFSFSTNVDEAFPPVSFEFEDS--VKLTVYPHDYLFTLEEELYCFGWQAGGLTTDE 411

Query: 397 LGNVNVIGDISLLDKIVVYDNERQQIGWAPANCN 404
              V ++GD+ L +K+VVYD + + IGWA  NC+
Sbjct: 412 RSEVILLGDLVLSNKLVVYDLDNEVIGWADHNCS 427

BLAST of MC11g0708 vs. ExPASy Swiss-Prot
Match: Q4V3D2 (Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 4.4e-31
Identity = 110/431 (25.52%), Postives = 187/431 (43.39%), Query Frame = 0

Query: 9   VSILFAIFAVSFSDKFSLEDRMQSGSGSN------RFASSVAFPIKGN--VYPLGHFTVS 68
           + ++   F  + + KF+ +++  S   S+      R  +++  P+ G+     +G +   
Sbjct: 22  IQVVSGNFVFNVTHKFAGKEKQLSELKSHDSFRHARMLANIDLPLGGDSRADSIGLYFTK 81

Query: 69  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPR---------DKQYRPHSNAVRCAE 128
           + +G+PPK + + +DTGSD+ WV C APC  C +           D +    S  V C +
Sbjct: 82  IKLGSPPKEYYVQVDTGSDILWVNC-APCPKCPVKTDLGIPLSLYDSKTSSTSKNVGCED 141

Query: 129 PLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSV----FAPNL 188
             CS +        K P   C Y V Y D  +S G  ++D   +    G++     A  +
Sbjct: 142 DFCSFIMQSETCGAKKP---CSYHVVYGDGSTSDGDFIKDNITLEQVTGNLRTAPLAQEV 201

Query: 189 GFGCGYDQ-----KHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFF 248
            FGCG +Q     +   +  G++G G    ++ SQL+A G  + +  HCL    GG +F 
Sbjct: 202 VFGCGKNQSGQLGQTDSAVDGIMGFGQSNTSIISQLAAGGSTKRIFSHCLDNMNGGGIFA 261

Query: 249 GGDLVPSSGMSWTPIMPS--------PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSY 308
            G+ V S  +  TPI+P+         G      P ++     +    G T+  DSG++ 
Sbjct: 262 VGE-VESPVVKTTPIVPNQVHYNVILKGMDVDGDPIDLPPSLASTNGDGGTI-IDSGTTL 321

Query: 309 TYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSF 368
            Y    +Y +++  +    + K          L +  +    F    +    F  + L F
Sbjct: 322 AYLPQNLYNSLIEKITAKQQVK----------LHMVQETFACFSFTSNTDKAFPVVNLHF 381

Query: 369 TDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVI--GDISLLDKIVVYDNER 404
            DS  ++  + P  YL   +    CFG  +G        +VI  GD+ L +K+VVYD E 
Sbjct: 382 EDS--LKLSVYPHDYLFSLREDMYCFGWQSGGMTTQDGADVILLGDLVLSNKLVVYDLEN 434

BLAST of MC11g0708 vs. NCBI nr
Match: XP_022132194.1 (aspartic proteinase Asp1-like isoform X4 [Momordica charantia])

HSP 1 Score: 842 bits (2175), Expect = 1.84e-308
Identity = 408/408 (100.00%), Postives = 408/408 (100.00%), Query Frame = 0

Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120
           VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120

Query: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS 180
           GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS
Sbjct: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS 180

Query: 181 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 240
           TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS
Sbjct: 181 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 240

Query: 241 PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD 300
           PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD
Sbjct: 241 PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD 300

Query: 301 EPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF 360
           EPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF
Sbjct: 301 EPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF 360

Query: 361 GILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK 408
           GILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK
Sbjct: 361 GILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK 408

BLAST of MC11g0708 vs. NCBI nr
Match: XP_022132187.1 (aspartic proteinase Asp1-like isoform X3 [Momordica charantia])

HSP 1 Score: 827 bits (2136), Expect = 4.70e-302
Identity = 408/436 (93.58%), Postives = 408/436 (93.58%), Query Frame = 0

Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL-------------------------- 120
           VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL                          
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLVSLIQIDGLFDSCFLFFYRNFVPSMT 120

Query: 121 --PRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180
             PRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL
Sbjct: 121 LQPRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180

Query: 181 FPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL 240
           FPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL
Sbjct: 181 FPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL 240

Query: 241 SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS 300
           SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS
Sbjct: 241 SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS 300

Query: 301 SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLAL 360
           SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLAL
Sbjct: 301 SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLAL 360

Query: 361 SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNER 408
           SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNER
Sbjct: 361 SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNER 420

BLAST of MC11g0708 vs. NCBI nr
Match: XP_022132180.1 (aspartic proteinase Asp1-like isoform X2 [Momordica charantia])

HSP 1 Score: 824 bits (2128), Expect = 1.05e-300
Identity = 408/444 (91.89%), Postives = 408/444 (91.89%), Query Frame = 0

Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120
           VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120

Query: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS 180
           GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS
Sbjct: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS 180

Query: 181 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 240
           TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS
Sbjct: 181 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 240

Query: 241 PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD 300
           PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD
Sbjct: 241 PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD 300

Query: 301 EPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF 360
           EPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF
Sbjct: 301 EPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF 360

Query: 361 GILNGSQVGLGNVNVIG------------------------------------DISLLDK 408
           GILNGSQVGLGNVNVIG                                    DISLLDK
Sbjct: 361 GILNGSQVGLGNVNVIGGKMRNTVGSTKNHFQVLFFYMLSSNISTSFSPKFVTDISLLDK 420

BLAST of MC11g0708 vs. NCBI nr
Match: XP_022132155.1 (aspartic proteinase Asp1-like isoform X1 [Momordica charantia] >XP_022132164.1 aspartic proteinase Asp1-like isoform X1 [Momordica charantia] >XP_022132172.1 aspartic proteinase Asp1-like isoform X1 [Momordica charantia])

HSP 1 Score: 809 bits (2089), Expect = 2.66e-294
Identity = 408/472 (86.44%), Postives = 408/472 (86.44%), Query Frame = 0

Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL-------------------------- 120
           VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL                          
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLVSLIQIDGLFDSCFLFFYRNFVPSMT 120

Query: 121 --PRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180
             PRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL
Sbjct: 121 LQPRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180

Query: 181 FPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL 240
           FPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL
Sbjct: 181 FPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL 240

Query: 241 SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS 300
           SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS
Sbjct: 241 SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS 300

Query: 301 SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLAL 360
           SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLAL
Sbjct: 301 SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLAL 360

Query: 361 SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIG--------------- 408
           SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIG               
Sbjct: 361 SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGGKMRNTVGSTKNHFQ 420

BLAST of MC11g0708 vs. NCBI nr
Match: XP_004142705.1 (aspartic proteinase Asp1 [Cucumis sativus])

HSP 1 Score: 662 bits (1707), Expect = 4.06e-237
Identity = 317/413 (76.76%), Postives = 355/413 (85.96%), Query Frame = 0

Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MH+  L P+S+LF IF++SF   F L D     S  N F SS+  P+KGNVYPLGHFTVS
Sbjct: 1   MHAFNLSPLSLLFLIFSLSFCGTFCLAD--WKSSAVNPFDSSILLPVKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120
           V IGNPPK+FELDIDTGSDLTWVQCDAPCTGCTLP D+ Y+PH+N VRC EPLCSALF  
Sbjct: 61  VTIGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPHDRLYKPHNNVVRCGEPLCSALFSA 120

Query: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS 180
            K PCKNPNDQCDY+VEYAD GSSIGVLV+D  P+RLTNG++ APNLGFGCGYDQ +GGS
Sbjct: 121 SKSPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTILAPNLGFGCGYDQHNGGS 180

Query: 181 -----TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWT 240
                TAGVLGLG+ KATM +QLSAL HVRNV+GHC SGQGGGFLFFGGDLVPSSGMSW 
Sbjct: 181 QLPPLTAGVLGLGNSKATMATQLSALSHVRNVLGHCFSGQGGGFLFFGGDLVPSSGMSWM 240

Query: 241 PIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKG 300
           PI+ +PGG+YS+GPAEVYFGG   GIRGL LTFDSGSSYTYFNSQVYGAVLNLL+N LKG
Sbjct: 241 PILRTPGGKYSAGPAEVYFGGNPVGIRGLILTFDSGSSYTYFNSQVYGAVLNLLRNGLKG 300

Query: 301 KPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKF 360
           +PL D P+DKTLP+CWKG+KAF+SV DVRNFFKPLALSF +SK VQFQIPPEAYLIIS  
Sbjct: 301 QPLRDAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSFGNSK-VQFQIPPEAYLIISNL 360

Query: 361 GNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK 408
           GNVC GILNGSQVGLGNVN+IGDIS+LDK++VYDNERQQIGWAPANC++PP+K
Sbjct: 361 GNVCLGILNGSQVGLGNVNLIGDISMLDKMMVYDNERQQIGWAPANCSKPPRK 410

BLAST of MC11g0708 vs. ExPASy TrEMBL
Match: A0A6J1BSD8 (aspartic proteinase Asp1-like isoform X4 OS=Momordica charantia OX=3673 GN=LOC111005085 PE=3 SV=1)

HSP 1 Score: 842 bits (2175), Expect = 8.91e-309
Identity = 408/408 (100.00%), Postives = 408/408 (100.00%), Query Frame = 0

Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120
           VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120

Query: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS 180
           GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS
Sbjct: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS 180

Query: 181 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 240
           TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS
Sbjct: 181 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 240

Query: 241 PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD 300
           PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD
Sbjct: 241 PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD 300

Query: 301 EPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF 360
           EPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF
Sbjct: 301 EPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF 360

Query: 361 GILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK 408
           GILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK
Sbjct: 361 GILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK 408

BLAST of MC11g0708 vs. ExPASy TrEMBL
Match: A0A6J1BT55 (aspartic proteinase Asp1-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111005085 PE=3 SV=1)

HSP 1 Score: 827 bits (2136), Expect = 2.28e-302
Identity = 408/436 (93.58%), Postives = 408/436 (93.58%), Query Frame = 0

Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL-------------------------- 120
           VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL                          
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLVSLIQIDGLFDSCFLFFYRNFVPSMT 120

Query: 121 --PRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180
             PRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL
Sbjct: 121 LQPRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180

Query: 181 FPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL 240
           FPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL
Sbjct: 181 FPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL 240

Query: 241 SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS 300
           SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS
Sbjct: 241 SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS 300

Query: 301 SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLAL 360
           SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLAL
Sbjct: 301 SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLAL 360

Query: 361 SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNER 408
           SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNER
Sbjct: 361 SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNER 420

BLAST of MC11g0708 vs. ExPASy TrEMBL
Match: A0A6J1BRQ3 (aspartic proteinase Asp1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005085 PE=3 SV=1)

HSP 1 Score: 824 bits (2128), Expect = 5.10e-301
Identity = 408/444 (91.89%), Postives = 408/444 (91.89%), Query Frame = 0

Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120
           VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120

Query: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS 180
           GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS
Sbjct: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS 180

Query: 181 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 240
           TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS
Sbjct: 181 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 240

Query: 241 PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD 300
           PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD
Sbjct: 241 PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD 300

Query: 301 EPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF 360
           EPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF
Sbjct: 301 EPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF 360

Query: 361 GILNGSQVGLGNVNVIG------------------------------------DISLLDK 408
           GILNGSQVGLGNVNVIG                                    DISLLDK
Sbjct: 361 GILNGSQVGLGNVNVIGGKMRNTVGSTKNHFQVLFFYMLSSNISTSFSPKFVTDISLLDK 420

BLAST of MC11g0708 vs. ExPASy TrEMBL
Match: A0A6J1BT39 (aspartic proteinase Asp1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005085 PE=3 SV=1)

HSP 1 Score: 809 bits (2089), Expect = 1.29e-294
Identity = 408/472 (86.44%), Postives = 408/472 (86.44%), Query Frame = 0

Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL-------------------------- 120
           VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL                          
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLVSLIQIDGLFDSCFLFFYRNFVPSMT 120

Query: 121 --PRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180
             PRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL
Sbjct: 121 LQPRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180

Query: 181 FPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL 240
           FPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL
Sbjct: 181 FPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL 240

Query: 241 SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS 300
           SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS
Sbjct: 241 SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS 300

Query: 301 SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLAL 360
           SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLAL
Sbjct: 301 SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLAL 360

Query: 361 SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIG--------------- 408
           SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIG               
Sbjct: 361 SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGGKMRNTVGSTKNHFQ 420

BLAST of MC11g0708 vs. ExPASy TrEMBL
Match: A0A0A0L366 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G361850 PE=3 SV=1)

HSP 1 Score: 662 bits (1707), Expect = 1.96e-237
Identity = 317/413 (76.76%), Postives = 355/413 (85.96%), Query Frame = 0

Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MH+  L P+S+LF IF++SF   F L D     S  N F SS+  P+KGNVYPLGHFTVS
Sbjct: 1   MHAFNLSPLSLLFLIFSLSFCGTFCLAD--WKSSAVNPFDSSILLPVKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120
           V IGNPPK+FELDIDTGSDLTWVQCDAPCTGCTLP D+ Y+PH+N VRC EPLCSALF  
Sbjct: 61  VTIGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPHDRLYKPHNNVVRCGEPLCSALFSA 120

Query: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS 180
            K PCKNPNDQCDY+VEYAD GSSIGVLV+D  P+RLTNG++ APNLGFGCGYDQ +GGS
Sbjct: 121 SKSPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTILAPNLGFGCGYDQHNGGS 180

Query: 181 -----TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWT 240
                TAGVLGLG+ KATM +QLSAL HVRNV+GHC SGQGGGFLFFGGDLVPSSGMSW 
Sbjct: 181 QLPPLTAGVLGLGNSKATMATQLSALSHVRNVLGHCFSGQGGGFLFFGGDLVPSSGMSWM 240

Query: 241 PIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKG 300
           PI+ +PGG+YS+GPAEVYFGG   GIRGL LTFDSGSSYTYFNSQVYGAVLNLL+N LKG
Sbjct: 241 PILRTPGGKYSAGPAEVYFGGNPVGIRGLILTFDSGSSYTYFNSQVYGAVLNLLRNGLKG 300

Query: 301 KPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKF 360
           +PL D P+DKTLP+CWKG+KAF+SV DVRNFFKPLALSF +SK VQFQIPPEAYLIIS  
Sbjct: 301 QPLRDAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSFGNSK-VQFQIPPEAYLIISNL 360

Query: 361 GNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK 408
           GNVC GILNGSQVGLGNVN+IGDIS+LDK++VYDNERQQIGWAPANC++PP+K
Sbjct: 361 GNVCLGILNGSQVGLGNVNLIGDISMLDKMMVYDNERQQIGWAPANCSKPPRK 410

BLAST of MC11g0708 vs. TAIR 10
Match: AT1G44130.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 477.2 bits (1227), Expect = 1.3e-134
Identity = 215/373 (57.64%), Postives = 284/373 (76.14%), Query Frame = 0

Query: 41  SSVAFPIKGNVYPLGHFTVSVNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQY 100
           SSV FP+ GNV+PLG+++V + IG+PPK F+ DIDTGSDLTWVQCDAPC+GCTLP + QY
Sbjct: 33  SSVVFPLSGNVFPLGYYSVLMQIGSPPKAFQFDIDTGSDLTWVQCDAPCSGCTLPPNLQY 92

Query: 101 RPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNG 160
           +P  N + C+ P+C+AL +P K  C NP +QCDY+V+YADQGSS+G LV D FP++L NG
Sbjct: 93  KPKGNIIPCSNPICTALHWPNKPHCPNPQEQCDYEVKYADQGSSMGALVTDQFPLKLVNG 152

Query: 161 SVFAPNLGFGCGYDQKHGG-----STAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQ 220
           S   P + FGCGYDQ +       +TAGVLGLG GK  + +QL + G  RNVVGHCLS +
Sbjct: 153 SFMQPPVAFGCGYDQSYPSAHPPPATAGVLGLGRGKIGLLTQLVSAGLTRNVVGHCLSSK 212

Query: 221 GGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYT 280
           GGGFLFFG +LVPS G++WTP++ S    Y++GPA++ F GK  G++GL L FD+GSSYT
Sbjct: 213 GGGFLFFGDNLVPSIGVAWTPLL-SQDNHYTTGPADLLFNGKPTGLKGLKLIFDTGSSYT 272

Query: 281 YFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFT 340
           YFNS+ Y  ++NL+ NDLK  PL    +DKTLP+CWKG K F+SV++V+NFFK + ++FT
Sbjct: 273 YFNSKAYQTIINLIGNDLKVSPLKVAKEDKTLPICWKGAKPFKSVLEVKNFFKTITINFT 332

Query: 341 DS-KNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQ 400
           +  +N Q  + PE YLI+SK GNVC G+LNGS+VGL N NVIGDIS+   +++YDNE+QQ
Sbjct: 333 NGRRNTQLYLAPELYLIVSKTGNVCLGLLNGSEVGLQNSNVIGDISMQGLMMIYDNEKQQ 392

Query: 401 IGWAPANCNRPPK 408
           +GW  ++CN+ PK
Sbjct: 393 LGWVSSDCNKLPK 404

BLAST of MC11g0708 vs. TAIR 10
Match: AT1G77480.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 453.4 bits (1165), Expect = 2.0e-127
Identity = 216/409 (52.81%), Postives = 289/409 (70.66%), Query Frame = 0

Query: 6   LFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVSVNIGN 65
           +F V IL A F  S + K   +   Q    + R +S+V FP+ GNVYPLG++ V +NIGN
Sbjct: 19  VFYVFILCARFQTSEATK---DSSAQVKLQNRRLSSTVVFPVSGNVYPLGYYYVLLNIGN 78

Query: 66  PPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFPGKVPC 125
           PPK+F+LDIDTGSDLTWVQCDAPC GCT PR KQY+P+ N + C+  LCS L  P   PC
Sbjct: 79  PPKLFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQYKPNHNTLPCSHILCSGLDLPQDRPC 138

Query: 126 KNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGG-----S 185
            +P DQCDY++ Y+D  SSIG LV D  P++L NGS+    L FGCGYDQ++ G      
Sbjct: 139 ADPEDQCDYEIGYSDHASSIGALVTDEVPLKLANGSIMNLRLTFGCGYDQQNPGPHPPPP 198

Query: 186 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMP- 245
           TAG+LGLG GK  +++QL +LG  +NV+ HCLS  G GFL  G +LVPSSG++WT +   
Sbjct: 199 TAGILGLGRGKVGLSTQLKSLGITKNVIVHCLSHTGKGFLSIGDELVPSSGVTWTSLATN 258

Query: 246 SPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLS 305
           SP   Y +GPAE+ F  K  G++G+ + FDSGSSYTYFN++ Y A+L+L++ DL GKPL+
Sbjct: 259 SPSKNYMAGPAELLFNDKTTGVKGINVVFDSGSSYTYFNAEAYQAILDLIRKDLNGKPLT 318

Query: 306 DEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQ-FQIPPEAYLIISKFGNV 365
           D   DK+LPVCWKG K  +S+ +V+ +FK + L F + KN Q FQ+PPE+YLII++ G V
Sbjct: 319 DTKDDKSLPVCWKGKKPLKSLDEVKKYFKTITLRFGNQKNGQLFQVPPESYLIITEKGRV 378

Query: 366 CFGILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPK 408
           C GILNG+++GL   N+IGDIS    +V+YDNE+Q+IGW  ++C++ PK
Sbjct: 379 CLGILNGTEIGLEGYNIIGDISFQGIMVIYDNEKQRIGWISSDCDKLPK 424

BLAST of MC11g0708 vs. TAIR 10
Match: AT1G77480.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 451.4 bits (1160), Expect = 7.5e-127
Identity = 215/408 (52.70%), Postives = 288/408 (70.59%), Query Frame = 0

Query: 6   LFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVSVNIGN 65
           +F V IL A F  S + K   +   Q    + R +S+V FP+ GNVYPLG++ V +NIGN
Sbjct: 19  VFYVFILCARFQTSEATK---DSSAQVKLQNRRLSSTVVFPVSGNVYPLGYYYVLLNIGN 78

Query: 66  PPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFPGKVPC 125
           PPK+F+LDIDTGSDLTWVQCDAPC GCT PR KQY+P+ N + C+  LCS L  P   PC
Sbjct: 79  PPKLFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQYKPNHNTLPCSHILCSGLDLPQDRPC 138

Query: 126 KNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGG-----S 185
            +P DQCDY++ Y+D  SSIG LV D  P++L NGS+    L FGCGYDQ++ G      
Sbjct: 139 ADPEDQCDYEIGYSDHASSIGALVTDEVPLKLANGSIMNLRLTFGCGYDQQNPGPHPPPP 198

Query: 186 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMP- 245
           TAG+LGLG GK  +++QL +LG  +NV+ HCLS  G GFL  G +LVPSSG++WT +   
Sbjct: 199 TAGILGLGRGKVGLSTQLKSLGITKNVIVHCLSHTGKGFLSIGDELVPSSGVTWTSLATN 258

Query: 246 SPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLS 305
           SP   Y +GPAE+ F  K  G++G+ + FDSGSSYTYFN++ Y A+L+L++ DL GKPL+
Sbjct: 259 SPSKNYMAGPAELLFNDKTTGVKGINVVFDSGSSYTYFNAEAYQAILDLIRKDLNGKPLT 318

Query: 306 DEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQ-FQIPPEAYLIISKFGNV 365
           D   DK+LPVCWKG K  +S+ +V+ +FK + L F + KN Q FQ+PPE+YLII++ G V
Sbjct: 319 DTKDDKSLPVCWKGKKPLKSLDEVKKYFKTITLRFGNQKNGQLFQVPPESYLIITEKGRV 378

Query: 366 CFGILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPP 407
           C GILNG+++GL   N+IGDIS    +V+YDNE+Q+IGW  ++C++ P
Sbjct: 379 CLGILNGTEIGLEGYNIIGDISFQGIMVIYDNEKQRIGWISSDCDKLP 423

BLAST of MC11g0708 vs. TAIR 10
Match: AT4G33490.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 424.9 bits (1091), Expect = 7.5e-119
Identity = 208/406 (51.23%), Postives = 268/406 (66.01%), Query Frame = 0

Query: 7   FPVSILFAIFAVSFSDKFSLEDRMQSGSGS--NRFASSVAFPIKGNVYPLGHFTVSVNIG 66
           F + ++     + FS       R  +G      R  SSV FP+ GNVYPLG++ V++NIG
Sbjct: 8   FMIVLMVMSLVLGFSSAVDFRWRKTAGFSDRFTRAVSSVVFPVHGNVYPLGYYNVTINIG 67

Query: 67  NPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFPGKVP 126
            PP+ + LD+DTGSDLTW+QCDAPC  C       Y+P S+ + C +PLC AL       
Sbjct: 68  QPPRPYYLDLDTGSDLTWLQCDAPCVRCLEAPHPLYQPSSDLIPCNDPLCKALHLNSNQR 127

Query: 127 CKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGST--- 186
           C+ P +QCDY+VEYAD GSS+GVLVRD+F M  T G    P L  GCGYDQ  G S+   
Sbjct: 128 CETP-EQCDYEVEYADGGSSLGVLVRDVFSMNYTQGLRLTPRLALGCGYDQIPGASSHHP 187

Query: 187 -AGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 246
             GVLGLG GK ++ SQL + G+V+NV+GHCLS  GGG LFFG DL  SS +SWTP+   
Sbjct: 188 LDGVLGLGRGKVSILSQLHSQGYVKNVIGHCLSSLGGGILFFGDDLYDSSRVSWTPMSRE 247

Query: 247 PGGRYSSG-PAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLS 306
               YS     E+ FGG+  G++ L   FDSGSSYTYFNS+ Y AV  LLK +L GKPL 
Sbjct: 248 YSKHYSPAMGGELLFGGRTTGLKNLLTVFDSGSSYTYFNSKAYQAVTYLLKRELSGKPLK 307

Query: 307 DEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDS--KNVQFQIPPEAYLIISKFGN 366
           +   D TLP+CW+G + F S+ +V+ +FKPLALSF         F+IPPEAYLIIS  GN
Sbjct: 308 EARDDHTLPLCWQGRRPFMSIEEVKKYFKPLALSFKTGWRSKTLFEIPPEAYLIISMKGN 367

Query: 367 VCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCN 404
           VC GILNG+++GL N+N+IGDIS+ D++++YDNE+Q IGW P +C+
Sbjct: 368 VCLGILNGTEIGLQNLNLIGDISMQDQMIIYDNEKQSIGWMPVDCD 412

BLAST of MC11g0708 vs. TAIR 10
Match: AT4G33490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 386.0 bits (990), Expect = 3.9e-107
Identity = 194/380 (51.05%), Postives = 246/380 (64.74%), Query Frame = 0

Query: 7   FPVSILFAIFAVSFSDKFSLEDRMQSGSGS--NRFASSVAFPIKGNVYPLGHFTVSVNIG 66
           F + ++     + FS       R  +G      R  SSV FP+ GNVYPLG++ V++NIG
Sbjct: 5   FMIVLMVMSLVLGFSSAVDFRWRKTAGFSDRFTRAVSSVVFPVHGNVYPLGYYNVTINIG 64

Query: 67  NPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFPGKVP 126
            PP+ + LD+DTGSDLTW+QCDAPC  C       Y+P S+ + C +PLC AL       
Sbjct: 65  QPPRPYYLDLDTGSDLTWLQCDAPCVRCLEAPHPLYQPSSDLIPCNDPLCKALHLNSNQR 124

Query: 127 CKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGST--- 186
           C+ P +QCDY+VEYAD GSS+GVLVRD+F M  T G    P L  GCGYDQ  G S+   
Sbjct: 125 CETP-EQCDYEVEYADGGSSLGVLVRDVFSMNYTQGLRLTPRLALGCGYDQIPGASSHHP 184

Query: 187 -AGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 246
             GVLGLG GK ++ SQL + G+V+NV+GHCLS  GGG LFFG DL  SS +SWTP+   
Sbjct: 185 LDGVLGLGRGKVSILSQLHSQGYVKNVIGHCLSSLGGGILFFGDDLYDSSRVSWTPMSRE 244

Query: 247 PGGRYSSG-PAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLS 306
               YS     E+ FGG+  G++ L   FDSGSSYTYFNS+ Y AV  LLK +L GKPL 
Sbjct: 245 YSKHYSPAMGGELLFGGRTTGLKNLLTVFDSGSSYTYFNSKAYQAVTYLLKRELSGKPLK 304

Query: 307 DEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDS--KNVQFQIPPEAYLIISKFGN 366
           +   D TLP+CW+G + F S+ +V+ +FKPLALSF         F+IPPEAYLIIS  GN
Sbjct: 305 EARDDHTLPLCWQGRRPFMSIEEVKKYFKPLALSFKTGWRSKTLFEIPPEAYLIISMKGN 364

Query: 367 VCFGILNGSQVGLGNVNVIG 378
           VC GILNG+++GL N+N+IG
Sbjct: 365 VCLGILNGTEIGLQNLNLIG 383