MC11g0690 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g0690
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RING-type E3 ubiquitin transferase
Location	MC11: 5526289 .. 5528211 (+)
RNA-Seq Expression	MC11g0690
Synteny	MC11g0690

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAATTTTTTTTTTAAATGGTAAAACAGAAAATTGGTTATCAGCATTTTTAAAACTAAAAGAAATTGTTATCAAGTCTTCAAAATTTGAATATTTTGAAAACGGTGGATTACTATTAAATGTTTGACTACTAAGAAAGAAAATTTTAAAAATAAAAATTTAGTTTGAAAAAATAATGAGTCGCGAGAGGCAAAGTATTTTGTTTTGAAAATTGGATTTTGTGCCGACTACTAATAATCTTGTTCTTCTCTCCATTCCGTAACTCAGAGCTCAAACAGTAACACCATAAGGAAACTGTTTGTATCACTTTCTACTCATCCTTTTCTTTCTCTCGACGACACACAAATGGCCATCCCCATTGCAATACCACAGCAAGAATTCCTTTTGCAATTCCACAAAGCCATTCCTTTCTGTTTCTCATCTCCCTTCCCTTCCATCCCCCTTCATTTTCCAATATATAAAAACTTCCCATCTTCAGATTCCGTTTCCCAAACAGACAAAACTCCCATAATTCTCACCAACTTACCCTGCACGTACCATGTCACTGAGCCCACCTCGTGCAACAACCAACCCCAACATCAGGACCTTCGACCTCTACTGGTGCTACCAATGCCACCGCGCGGTCAGGCTCGCCGCCGCGCACTCCTCCGACCTCACCTGCCCGCGCTGTTTCGGGCACTTCCTCGAAGAGCTCCACGTCCCTCGCCCCACCCAGCTCGACCTCTCGCCGGAGGCCAGATTGCTCGAAGCCCTTTCGCTAATGCTCGACCCGCCGGTTCGGCTCGTGAATTTCAGACTCCCGGACCCGGAACATGAACCCCGCCGCCGTCCACTCTGGCAATTTTTCGACGATTTCGACAGGAGACCGCTTCGCGATCTGGAAGGAGACGATTCCCTCCGGTGGCGAACACGGCGCCGTAACCGGAGCCTGGATGAGAGGGAAAGTTCCGGCCTAGGACTAGAACCCCCAAGTCGGAACCAGCCTCGAACTTTCATCGTTATCAGACCCATAGACCAATCTCGGCCGATGCAGCCCCTAATTCCACCGGGCGCCAATCCAAGAGACTACTTTGCCGGACCACGACTGGACGACTTAATCGAAGAACTGACGCAGAACGACCGACCGGGGCCGCCGCCGGCGAGCGATGCGGCGATCGAGGGGATACCGACGGTGAAGATAGTGGCGGAGCATCTGAAGAACGATTCGCACTGTCCGGTTTGTAAGGAGGAGTTTGAGGTGGGAGGAGAAGCGAGGGAATTGAACTGCAAGCACATATACCATTCGGACTGCATCGTGCCATGGCTAAGGCTCCATAACTCTTGTCCGGTGTGCAGGCAAGAGATGCCGCCATTGCCGCCAGCGCCGGAGAATCGAGGACCGGGAAGTTCTCAGGACGGCGAAGGGATTGGGAGAAGATGTGCCCGTTGGTGGGGCCAGTTGGTTTCTAGGCCATTTCGAAATAGGTATCGCCAGATTACCCCTCACGACCAGGAGAACATTCCTCCTCGAGGTGATGCCTGAATTATATCTTTTATTCAGAAATTACTATATATATGTTCTAATATTTGTAGTTATAAAAAAAATTTGTATGGGTGATATATTATTAACATTCATTATCGTTCTAATGGGCTAACAAAAGAAAACCATGTGTTGAGCTTTCTTTGTAAGAGGATCATCAAAATACTGATATTCTAAAACTCAGACGAAAGAAATCAGTTGATATATTTTCAAAAAACAAAATTATTTTATACTTTATTCTAAATTGAAAATAAAAGCTTGGGAGATTGAGATTCTGACTCAGGTATTTTGTTCCATTCTCCAGAAGTAAATTCGTGGGGTGGTTCAATCTACCTCTCTTCCTTGGCCCTTCTAATGTCGATGTTTATGCTGCAAGTAACCTGGTTTTTACTTGAACCTCTTCGT

mRNA sequence

GAAATTTTTTTTTTAAATGGTAAAACAGAAAATTGGTTATCAGCATTTTTAAAACTAAAAGAAATTGTTATCAAGTCTTCAAAATTTGAATATTTTGAAAACGGTGGATTACTATTAAATGTTTGACTACTAAGAAAGAAAATTTTAAAAATAAAAATTTAGTTTGAAAAAATAATGAGTCGCGAGAGGCAAAGTATTTTGTTTTGAAAATTGGATTTTGTGCCGACTACTAATAATCTTGTTCTTCTCTCCATTCCGTAACTCAGAGCTCAAACAGTAACACCATAAGGAAACTGTTTGTATCACTTTCTACTCATCCTTTTCTTTCTCTCGACGACACACAAATGGCCATCCCCATTGCAATACCACAGCAAGAATTCCTTTTGCAATTCCACAAAGCCATTCCTTTCTGTTTCTCATCTCCCTTCCCTTCCATCCCCCTTCATTTTCCAATATATAAAAACTTCCCATCTTCAGATTCCGTTTCCCAAACAGACAAAACTCCCATAATTCTCACCAACTTACCCTGCACGTACCATGTCACTGAGCCCACCTCGTGCAACAACCAACCCCAACATCAGGACCTTCGACCTCTACTGGTGCTACCAATGCCACCGCGCGGTCAGGCTCGCCGCCGCGCACTCCTCCGACCTCACCTGCCCGCGCTGTTTCGGGCACTTCCTCGAAGAGCTCCACGTCCCTCGCCCCACCCAGCTCGACCTCTCGCCGGAGGCCAGATTGCTCGAAGCCCTTTCGCTAATGCTCGACCCGCCGGTTCGGCTCGTGAATTTCAGACTCCCGGACCCGGAACATGAACCCCGCCGCCGTCCACTCTGGCAATTTTTCGACGATTTCGACAGGAGACCGCTTCGCGATCTGGAAGGAGACGATTCCCTCCGGTGGCGAACACGGCGCCGTAACCGGAGCCTGGATGAGAGGGAAAGTTCCGGCCTAGGACTAGAACCCCCAAGTCGGAACCAGCCTCGAACTTTCATCGTTATCAGACCCATAGACCAATCTCGGCCGATGCAGCCCCTAATTCCACCGGGCGCCAATCCAAGAGACTACTTTGCCGGACCACGACTGGACGACTTAATCGAAGAACTGACGCAGAACGACCGACCGGGGCCGCCGCCGGCGAGCGATGCGGCGATCGAGGGGATACCGACGGTGAAGATAGTGGCGGAGCATCTGAAGAACGATTCGCACTGTCCGGTTTGTAAGGAGGAGTTTGAGGTGGGAGGAGAAGCGAGGGAATTGAACTGCAAGCACATATACCATTCGGACTGCATCGTGCCATGGCTAAGGCTCCATAACTCTTGTCCGGTGTGCAGGCAAGAGATGCCGCCATTGCCGCCAGCGCCGGAGAATCGAGGACCGGGAAGTTCTCAGGACGGCGAAGGGATTGGGAGAAGATGTGCCCGTTGGTGGGGCCAGTTGGTTTCTAGGCCATTTCGAAATAGGTATCGCCAGATTACCCCTCACGACCAGGAGAACATTCCTCCTCGAGGTATTTTGTTCCATTCTCCAGAAGTAAATTCGTGGGGTGGTTCAATCTACCTCTCTTCCTTGGCCCTTCTAATGTCGATGTTTATGCTGCAAGTAACCTGGTTTTTACTTGAACCTCTTCGT

Coding sequence (CDS)

ATGTCACTGAGCCCACCTCGTGCAACAACCAACCCCAACATCAGGACCTTCGACCTCTACTGGTGCTACCAATGCCACCGCGCGGTCAGGCTCGCCGCCGCGCACTCCTCCGACCTCACCTGCCCGCGCTGTTTCGGGCACTTCCTCGAAGAGCTCCACGTCCCTCGCCCCACCCAGCTCGACCTCTCGCCGGAGGCCAGATTGCTCGAAGCCCTTTCGCTAATGCTCGACCCGCCGGTTCGGCTCGTGAATTTCAGACTCCCGGACCCGGAACATGAACCCCGCCGCCGTCCACTCTGGCAATTTTTCGACGATTTCGACAGGAGACCGCTTCGCGATCTGGAAGGAGACGATTCCCTCCGGTGGCGAACACGGCGCCGTAACCGGAGCCTGGATGAGAGGGAAAGTTCCGGCCTAGGACTAGAACCCCCAAGTCGGAACCAGCCTCGAACTTTCATCGTTATCAGACCCATAGACCAATCTCGGCCGATGCAGCCCCTAATTCCACCGGGCGCCAATCCAAGAGACTACTTTGCCGGACCACGACTGGACGACTTAATCGAAGAACTGACGCAGAACGACCGACCGGGGCCGCCGCCGGCGAGCGATGCGGCGATCGAGGGGATACCGACGGTGAAGATAGTGGCGGAGCATCTGAAGAACGATTCGCACTGTCCGGTTTGTAAGGAGGAGTTTGAGGTGGGAGGAGAAGCGAGGGAATTGAACTGCAAGCACATATACCATTCGGACTGCATCGTGCCATGGCTAAGGCTCCATAACTCTTGTCCGGTGTGCAGGCAAGAGATGCCGCCATTGCCGCCAGCGCCGGAGAATCGAGGACCGGGAAGTTCTCAGGACGGCGAAGGGATTGGGAGAAGATGTGCCCGTTGGTGGGGCCAGTTGGTTTCTAGGCCATTTCGAAATAGGTATCGCCAGATTACCCCTCACGACCAGGAGAACATTCCTCCTCGAGGTATTTTGTTCCATTCTCCAGAAGTAAATTCGTGGGGTGGTTCAATCTACCTCTCTTCCTTGGCCCTTCTAATGTCGATGTTTATGCTGCAAGTAACCTGGTTTTTACTTGAACCTCTTCGT

Protein sequence

MSLSPPRATTNPNIRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPTQLDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDDSLRWRTRRRNRSLDERESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFAGPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEARELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWWGQLVSRPFRNRYRQITPHDQENIPPRGILFHSPEVNSWGGSIYLSSLALLMSMFMLQVTWFLLEPLR

Homology

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BLAST of MC11g0690 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.3e-37
Identity = 99/280 (35.36%), Postives = 146/280 (52.14%), Query Frame = 0


Query: 19  LYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPTQ-LDLSPEARLLEALSLMLD 78
           +++CYQC++ V ++ + S+D  CP C   FLEE   P P Q L+ +P +   ++   M D
Sbjct: 20  MFFCYQCNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSS--DSFFPMAD 79

Query: 79  PPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGD---DSLRWRTRRRNRSLDER 138
           P   L+          P        F    +   R  + +   D+    T  +N  L   
Sbjct: 80  PFSTLLPLIFGSSAAAPSGMDFMSLFGPSMQPQARSTQQNPQSDAFDPFTFLQNH-LQTL 139

Query: 139 ESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFAGPRLDDLIEELTQND 198
            SSG   E         F++     ++ P  P      N  DYF GP L+ LI++L +ND
Sbjct: 140 RSSGTHFE---------FVI-----ENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAEND 199

Query: 199 --RPGPPPASDAAIEGIPTVKIVAEHLKND-SHCPVCKEEFEVGGEARELNCKHIYHSDC 258
             R G PPAS +AI+ +PTVK+  + LK++ + C VC +EFE G + +++ CKH++H DC
Sbjct: 200 PNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDC 259

Query: 259 IVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIG 292
           ++PWL LHNSCPVCR E+P   P  ENR  GS   G+G G
Sbjct: 260 LLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSGDGQG 282

[Close]

BLAST of MC11g0690 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 6.9e-36
Identity = 102/282 (36.17%), Postives = 140/282 (49.65%), Query Frame = 0


Query: 20  YWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPTQLDLSPEAR----------LL 79
           +WC++C RAVRL   H  +  C  C G F+EEL + + +  D+    R          L+
Sbjct: 8   HWCHRCQRAVRL---HGQEPVCFYCGGGFVEELDMAQASPFDMFRSHRGVVERDQTFDLM 67

Query: 80  EALSLMLDPPVRLVNFRLPDPEH--EPRRRPLWQFFDDF-DRRPLRDLEGDDSLRWRTRR 139
           +A S+ +         RL +  H  E R R +    ++F    PL    G    R     
Sbjct: 68  DAFSVFM-------RNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDN 127

Query: 140 RNRSLDERESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFAGPRLDDL 199
              +L    S G+G+                               N  DYF GP L++L
Sbjct: 128 AVEALFNGGSPGIGI----------------------------TRGNTGDYFFGPGLEEL 187

Query: 200 IEELTQ-NDRPGPPPASDAAIEGIPTVKIVAEHLK-NDSHCPVCKEEFEVGGEARELNCK 259
            E+L+    R GPPPA  +AI+ +PT+KI   HL+ +DS+CPVCK+EFE+G EA+++ C 
Sbjct: 188 FEQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCN 245

Query: 260 HIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQD 287
           HIYHSDCIVPWL  HNSCPVCRQE+      P   GP SSQ+
Sbjct: 248 HIYHSDCIVPWLVQHNSCPVCRQEL------PSASGPSSSQN 245

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BLAST of MC11g0690 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 4.5e-35
Identity = 106/297 (35.69%), Postives = 143/297 (48.15%), Query Frame = 0


Query: 20  YWCYQCHRAVRLA-AAHSSDLTCPRCFGHFLEELHVPRPTQ----LDLSPEARLLEALSL 79
           ++CYQC+R V +  +  SSD TCP C   FLEE   P P Q    L+ +P +     L L
Sbjct: 23  FFCYQCNRTVNVTISPPSSDPTCPICNEGFLEEYDNPNPNQGSGFLNPNPNSIPFHDLFL 82

Query: 80  ML-DPPVRLVNFRLPDPEH---------EPRRRPLWQFFDDFDRRPLRDLEGDDSLRWRT 139
            L DP   L+    P             +P    L          P R   GD       
Sbjct: 83  TLSDPFASLLPLLFPSSSSTTTSSSASIDPNNPSL--------SGPTRSGRGDPFAFDPF 142

Query: 140 RRRNRSLDERESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFAGPRLD 199
                 L++  SSG  +E         F++     Q+ P        AN  DYF GP L+
Sbjct: 143 TFIQNHLNDLRSSGAQIE---------FVI-----QNNPSDQGFRLPANIGDYFIGPGLE 202

Query: 200 DLIEELTQND--RPGPPPASDAAIEGIPTVKIVAEHLKND-SHCPVCKEEFEVGGEAREL 259
            LI++L +ND  R G PPAS +AIE +P V I   +L ++ + C VC ++FE G EA+++
Sbjct: 203 QLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQM 262

Query: 260 NCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENR-----GPGSSQDGEGIGRR 294
            CKH+YH DC++PWL LHNSCPVCR E+P   P  E R     G     DG+  G+R
Sbjct: 263 PCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYERRVRGAQGTSGGNDGDNSGQR 297

[Close]

BLAST of MC11g0690 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.1e-33
Identity = 105/290 (36.21%), Postives = 142/290 (48.97%), Query Frame = 0


Query: 20  YWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHV-PRPTQLDL--SPEARLLEALSLML 79
           +WC++C RAV L A    D  C  C G F+EE+ + P     D+   P   L+EA S  +
Sbjct: 8   HWCHRCQRAVWLRA---RDAVCSYCGGGFVEEIDIGPSRAHRDVERDPTFDLMEAFSAFM 67

Query: 80  DPPV------RLVNFRLPDPEHE--PRRRPLWQFFDDFDRRPLRDLEGDDSLRWRTRRRN 139
              +      R ++ RL     E      PL  F     + P R   GD+S         
Sbjct: 68  RSRLAERSYDREISGRLGSAGSESFSNLAPLLIF---GGQAPFRLAGGDNS-------SV 127

Query: 140 RSLDERESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFAGPRLDDLIE 199
            +     + G+G                           I  G N  DYF GP L++LIE
Sbjct: 128 EAFVNGAAPGIG---------------------------IARGTNAGDYFFGPGLEELIE 187

Query: 200 ELTQ-NDRPGPPPASDAAIEGIPTVKIVAEHLK-NDSHCPVCKEEFEVGGEARELNCKHI 259
           +L+      GPPPA  ++I+ +PT+KI  +HLK +DSHCPVCK+EFE+  EA+++ C HI
Sbjct: 188 QLSSGTHHRGPPPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHI 247

Query: 260 YHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCAR 297
           YHSDCIVPWL  HNSCPVCR+E   LP    +    SSQ+    GR  +R
Sbjct: 248 YHSDCIVPWLVQHNSCPVCRKE---LPSRGSSSSTQSSQNRSTNGRENSR 254

[Close]

BLAST of MC11g0690 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 8.5e-26
Identity = 94/311 (30.23%), Postives = 137/311 (44.05%), Query Frame = 0


Query: 1   MSLSPPRATTNPNIRTFDLYWCYQCHRAVRLAAAHSS----DLTCPRCFGHFLEELHVPR 60
           M  +P   TT   I     YWCY C R V + A   +     + CP C G F+E+++   
Sbjct: 1   MPSTPTTPTTTTPITA--SYWCYSCTRFVSVWADQGTTTVGSVACPHCDGGFIEQIN--- 60

Query: 61  PTQLDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPR--RRPLWQFFDDFDRRPLRDL 120
               D S  A     L++     VR +N          R  RRP +         P+  L
Sbjct: 61  ----DSSSAA---TELTIPASTEVRSINNNRRSVIRRRRSGRRPSF--------NPVIVL 120

Query: 121 EGDDSLRWRTRRRNRSLDER-------ESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPL 180
           +G    R      + + D R       + SG GL P                        
Sbjct: 121 QGGAGEREEGEEGDAARDRRAFEFYYDDGSGSGLRP------------------------ 180

Query: 181 IPPGANPRDYFAGPRLDDLIEELTQND-------RPGPPPASDAAIEGIPTVKIVAEHLK 240
           +P   +  +   G   + L+E+L+Q +       R G PPAS +AIE +P V+I   H+ 
Sbjct: 181 LPDSVS--EILMGSGFERLLEQLSQIEASATGIGRSGNPPASKSAIESLPRVEISDCHIG 240

Query: 241 NDSHCPVCKEEFEVGGEARELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRG 292
           ++++C VC E FE   EARE+ CKH++H DCIVPWL + NSCPVCR E+P  P    NR 
Sbjct: 241 SEANCAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSEP----NRR 261

[Close]

BLAST of MC11g0690 vs. NCBI nr
Match: KAG7024775.1 (E3 ubiquitin-protein ligase RZF1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 497 bits (1280), Expect = 9.16e-174
Identity = 253/365 (69.32%), Postives = 289/365 (79.18%), Query Frame = 0


Query: 1   MSLSPPRATTNP--NIRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPT 60
           M+LSPP  TTN   + RTF LYWC+QCHR V LA+A  SDLTCPRCFGHF+EE+H+P P 
Sbjct: 1   MALSPPPRTTNDRRHARTFHLYWCHQCHRTVTLASAEPSDLTCPRCFGHFVEEVHLPLP- 60

Query: 61  QLDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDD 120
           +LD SPE RLLEALSLMLDPPVRL N+R PDP+   RRRP    FDDFDRR   D EGD+
Sbjct: 61  ELDPSPETRLLEALSLMLDPPVRLFNYRHPDPD---RRRPPLHRFDDFDRRSFSDPEGDE 120

Query: 121 SLRWRTRRRNRSLDERESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYF 180
            L WRTRRR+RSLDER++SG   +PP+ NQ RT IV+ P DQ +P+QP+IP  ANPRDYF
Sbjct: 121 WLHWRTRRRSRSLDERDNSG--RQPPNPNQSRTVIVLGPPDQFQPIQPIIPRRANPRDYF 180

Query: 181 AGPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEA 240
            GPRLD+LIE+LTQNDRPGPPPA + AIE IPTVKI AEHLKNDSHCPVCKEEFEVGGEA
Sbjct: 181 TGPRLDELIEDLTQNDRPGPPPAGEEAIERIPTVKIAAEHLKNDSHCPVCKEEFEVGGEA 240

Query: 241 RELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWW 300
           REL+CKH+YHS+CIVPWLRLHNSCPVCRQE+PPLPP  EN   G+S++G  IGR C RWW
Sbjct: 241 RELSCKHLYHSECIVPWLRLHNSCPVCRQEIPPLPP--ENGAAGNSENGVRIGRACTRWW 300

Query: 301 GQLVSRPFRNRYRQITPHDQENIPPRGILFHS-PEVNSWGGSIYLSSLALLMSMFMLQVT 360
            +L S  FRNRYRQI+P      PP      S PE+NSWGGS YLSSLAL M+MF+LQ+T
Sbjct: 301 NRLASWLFRNRYRQISPSS----PPNRFNASSYPEINSWGGSSYLSSLALRMTMFLLQIT 353

Query: 361 WFLLE 362
           W LL 
Sbjct: 361 WILLH 353

[Close]

BLAST of MC11g0690 vs. NCBI nr
Match: XP_023535798.1 (E3 ubiquitin-protein ligase RZF1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 488 bits (1257), Expect = 2.78e-170
Identity = 249/364 (68.41%), Postives = 284/364 (78.02%), Query Frame = 0


Query: 1   MSLSPPRATTNP--NIRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPT 60
           MS +PP  TTN   + RTF LYWC+QCHR V LA+A  SDLTCPRCFGHF+EE+H+P P 
Sbjct: 1   MSFTPPPHTTNDRRHARTFHLYWCHQCHRTVTLASAQPSDLTCPRCFGHFVEEVHLPLP- 60

Query: 61  QLDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDD 120
           +LD SPE RLLEALSLMLDPPV L N+R P P+H   RRP    FDDFDRR   D EGD+
Sbjct: 61  ELDPSPETRLLEALSLMLDPPVCLFNYRHPVPDH---RRPPLNRFDDFDRRSFSDPEGDE 120

Query: 121 SLRWRTRRRNRSLDERESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYF 180
            L WRTRRR+RSLDER++SG   +PP+ NQ RT IV+ P DQ +P+QP+IP  ANPRDYF
Sbjct: 121 WLHWRTRRRSRSLDERDNSG--RQPPNPNQSRTVIVLGPPDQFQPIQPIIPRRANPRDYF 180

Query: 181 AGPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEA 240
            GPRLD+LIE+LTQNDRPGP PA + AIE IPTVKI AEHLKNDS CPVCKEEFEVGGEA
Sbjct: 181 TGPRLDELIEDLTQNDRPGPAPAGEEAIERIPTVKIAAEHLKNDSQCPVCKEEFEVGGEA 240

Query: 241 RELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWW 300
           REL+CKH+YHS+CIVPWLRLHNSCPVCRQE+PPLPP  EN   G+S++G  IGR C RWW
Sbjct: 241 RELSCKHLYHSECIVPWLRLHNSCPVCRQEIPPLPP--ENGAAGNSENGVRIGRACTRWW 300

Query: 301 GQLVSRPFRNRYRQITPHDQENIPPRGILFHSPEVNSWGGSIYLSSLALLMSMFMLQVTW 360
            +LVS  FRNRYRQI+P      PP        E+NSWGGS YLSSLALLM+MFMLQ+TW
Sbjct: 301 NRLVSWLFRNRYRQISPSS----PPNRFNASPSEINSWGGSSYLSSLALLMTMFMLQITW 352

Query: 361 FLLE 362
            LL 
Sbjct: 361 ILLH 352

[Close]

BLAST of MC11g0690 vs. NCBI nr
Match: XP_038898007.1 (E3 ubiquitin-protein ligase RZF1 isoform X1 [Benincasa hispida])

HSP 1 Score: 472 bits (1214), Expect = 9.27e-164
Identity = 247/366 (67.49%), Postives = 279/366 (76.23%), Query Frame = 0


Query: 1   MSLSPPRATTNP-NIRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPTQ 60
           MS SPP+ TTN  NI TF LYWC++CHR V  AAA  S+LTCPRCFG F+EE+H+  P Q
Sbjct: 1   MSFSPPQRTTNNHNITTFQLYWCHRCHRTVTHAAAPPSELTCPRCFGQFIEEIHLTLP-Q 60

Query: 61  LDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDDS 120
            D SP ARLLEALSLML+ P+RL+N   P+     RRRP    FDDFDRR   D EGD+ 
Sbjct: 61  FDPSPAARLLEALSLMLNQPIRLLNNGTPNAN---RRRPPLHRFDDFDRRSFSDPEGDEL 120

Query: 121 LRWRTRRRNRSLDERESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFA 180
             WRTR R RSLDER++SGL   PP+ N+ RT IV  P DQ +P+QP++P  A+PRDYF 
Sbjct: 121 PHWRTRWRGRSLDERDNSGL---PPNPNRSRTVIVFGPPDQLQPIQPILPRRADPRDYFT 180

Query: 181 GPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEAR 240
           GP+LD+LIEELTQNDRPG PPAS+ AIE IPTVKI AEHLKND HCP+CKEEFEVGGEAR
Sbjct: 181 GPQLDELIEELTQNDRPGQPPASEEAIERIPTVKITAEHLKNDVHCPICKEEFEVGGEAR 240

Query: 241 ELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWWG 300
           EL+CKHIYHS+CIVPWLRLHNSCPVCRQEMP L   PEN   GSS+D EGIGRRC RWW 
Sbjct: 241 ELSCKHIYHSECIVPWLRLHNSCPVCRQEMPLL--TPENGASGSSEDEEGIGRRCTRWWS 300

Query: 301 QLVSRPFRNRYRQITPHDQENIPPRGILFHSPEVNSWGGSIYLSSLALLMSMFMLQVTWF 360
           QLVS PFRNRYRQI+P       PR     S E+NS GG  YLSSL LLMSMF+LQ+TW 
Sbjct: 301 QLVSWPFRNRYRQISPM------PRNRFSASREINSRGGLSYLSSLVLLMSMFLLQITWI 351

Query: 361 LLEPLR 365
           LLEPLR
Sbjct: 361 LLEPLR 351

[Close]

BLAST of MC11g0690 vs. NCBI nr
Match: KAG6591901.1 (E3 ubiquitin-protein ligase RZF1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 458 bits (1179), Expect = 7.69e-159
Identity = 227/322 (70.50%), Postives = 259/322 (80.43%), Query Frame = 0


Query: 1   MSLSPPRATTNP--NIRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPT 60
           M+LSPP  TTN   + RTF LYWC+QCHR V LA+A  SDLTCPRCFGHF+EE+H+P P 
Sbjct: 1   MALSPPPRTTNDRRHARTFHLYWCHQCHRTVTLASAEPSDLTCPRCFGHFVEEVHLPLP- 60

Query: 61  QLDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDD 120
           +LD SPE RLLEALSLMLDPPVRL N+R PDP+   RRRP    FDDFDRR   D EGD+
Sbjct: 61  ELDPSPETRLLEALSLMLDPPVRLFNYRHPDPD---RRRPPLHRFDDFDRRSFSDPEGDE 120

Query: 121 SLRWRTRRRNRSLDERESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYF 180
            L WRTRRR+RSLDER++SG   +PP+ NQ RT IV+ P DQ +P+QP+IP  ANPRDYF
Sbjct: 121 WLHWRTRRRSRSLDERDNSG--RQPPNPNQSRTVIVLGPPDQFQPIQPIIPRRANPRDYF 180

Query: 181 AGPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEA 240
            GPRLD+LIE+LTQNDRPGPPPA + AIE IPTVKI AEHLKNDSHCPVCKEEFEVGGEA
Sbjct: 181 TGPRLDELIEDLTQNDRPGPPPAGEEAIERIPTVKIAAEHLKNDSHCPVCKEEFEVGGEA 240

Query: 241 RELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWW 300
           REL+CKH+YHS+CIVPWLRLHNSCPVCRQE+PPLPP  EN   G+S++G  IGR C RWW
Sbjct: 241 RELSCKHLYHSECIVPWLRLHNSCPVCRQEIPPLPP--ENGAAGNSENGVRIGRACTRWW 300

Query: 301 GQLVSRPFRNRYRQITPHDQEN 320
            +L S  FRNRYRQI+P    N
Sbjct: 301 NRLASWLFRNRYRQISPSSPPN 314

[Close]

BLAST of MC11g0690 vs. NCBI nr
Match: XP_022975873.1 (E3 ubiquitin-protein ligase RZF1-like [Cucurbita maxima])

HSP 1 Score: 456 bits (1174), Expect = 4.42e-158
Identity = 228/322 (70.81%), Postives = 258/322 (80.12%), Query Frame = 0


Query: 1   MSLSPPRATTNP--NIRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPT 60
           M+LSPP  TTN   + RTF LYWC+QCHR V LA+A  SDLTCPRCFGHF+EE+H+P P 
Sbjct: 1   MALSPPPHTTNDRRHARTFHLYWCHQCHRTVTLASAEPSDLTCPRCFGHFVEEVHLPLP- 60

Query: 61  QLDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDD 120
           +LD SPE RLLEALSLMLDPPVRL N+R PDP+   RRRP    FDDFDRR   D EGD+
Sbjct: 61  ELDPSPETRLLEALSLMLDPPVRLFNYRHPDPD---RRRPPLHRFDDFDRRSFSDPEGDE 120

Query: 121 SLRWRTRRRNRSLDERESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYF 180
            L WRTRRR+RSLDER++SG   +PP+ NQ RT IV+ P DQ +P+QP+IP  ANPRDYF
Sbjct: 121 WLHWRTRRRSRSLDERDNSG--RQPPNPNQSRTVIVLGPPDQFQPIQPIIPRRANPRDYF 180

Query: 181 AGPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEA 240
            GPRLD+LIE+LTQNDRPGPPPA + AIE IPTVKI AEHLKNDSHCPVCKEEFEVGGEA
Sbjct: 181 TGPRLDELIEDLTQNDRPGPPPAGEEAIERIPTVKIAAEHLKNDSHCPVCKEEFEVGGEA 240

Query: 241 RELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWW 300
           REL+CKHIYHS+CIVPWLRLHNSCPVCRQE+PPLPP  EN   G+S+    IGR C RWW
Sbjct: 241 RELSCKHIYHSECIVPWLRLHNSCPVCRQEIPPLPP--ENGAAGNSETEVRIGRACTRWW 300

Query: 301 GQLVSRPFRNRYRQITPHDQEN 320
            +LVS  FRNRYRQI+P    N
Sbjct: 301 NRLVSWLFRNRYRQISPSSPPN 314

[Close]

BLAST of MC11g0690 vs. ExPASy TrEMBL
Match: A0A6J1IKH7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111476446 PE=4 SV=1)

HSP 1 Score: 456 bits (1174), Expect = 2.14e-158
Identity = 228/322 (70.81%), Postives = 258/322 (80.12%), Query Frame = 0


Query: 1   MSLSPPRATTNP--NIRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPT 60
           M+LSPP  TTN   + RTF LYWC+QCHR V LA+A  SDLTCPRCFGHF+EE+H+P P 
Sbjct: 1   MALSPPPHTTNDRRHARTFHLYWCHQCHRTVTLASAEPSDLTCPRCFGHFVEEVHLPLP- 60

Query: 61  QLDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDD 120
           +LD SPE RLLEALSLMLDPPVRL N+R PDP+   RRRP    FDDFDRR   D EGD+
Sbjct: 61  ELDPSPETRLLEALSLMLDPPVRLFNYRHPDPD---RRRPPLHRFDDFDRRSFSDPEGDE 120

Query: 121 SLRWRTRRRNRSLDERESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYF 180
            L WRTRRR+RSLDER++SG   +PP+ NQ RT IV+ P DQ +P+QP+IP  ANPRDYF
Sbjct: 121 WLHWRTRRRSRSLDERDNSG--RQPPNPNQSRTVIVLGPPDQFQPIQPIIPRRANPRDYF 180

Query: 181 AGPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEA 240
            GPRLD+LIE+LTQNDRPGPPPA + AIE IPTVKI AEHLKNDSHCPVCKEEFEVGGEA
Sbjct: 181 TGPRLDELIEDLTQNDRPGPPPAGEEAIERIPTVKIAAEHLKNDSHCPVCKEEFEVGGEA 240

Query: 241 RELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWW 300
           REL+CKHIYHS+CIVPWLRLHNSCPVCRQE+PPLPP  EN   G+S+    IGR C RWW
Sbjct: 241 RELSCKHIYHSECIVPWLRLHNSCPVCRQEIPPLPP--ENGAAGNSETEVRIGRACTRWW 300

Query: 301 GQLVSRPFRNRYRQITPHDQEN 320
            +LVS  FRNRYRQI+P    N
Sbjct: 301 NRLVSWLFRNRYRQISPSSPPN 314

[Close]

BLAST of MC11g0690 vs. ExPASy TrEMBL
Match: A0A6J1FAA6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111443524 PE=4 SV=1)

HSP 1 Score: 455 bits (1171), Expect = 6.12e-158
Identity = 226/322 (70.19%), Postives = 258/322 (80.12%), Query Frame = 0


Query: 1   MSLSPPRATTNP--NIRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPT 60
           M+LSPP  TTN   + RTF LYWC+QCHR V LA+A  SDLTCPRCFGHF+EE+H+P P 
Sbjct: 1   MALSPPPRTTNDRRHARTFHLYWCHQCHRTVTLASAEPSDLTCPRCFGHFVEEVHLPLP- 60

Query: 61  QLDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDD 120
           +LD SPE RLLEALSLMLDPPVRL N+R PDP+   RRRP    FDDFDRR   D EGD+
Sbjct: 61  ELDPSPETRLLEALSLMLDPPVRLFNYRHPDPD---RRRPPLHRFDDFDRRSFSDPEGDE 120

Query: 121 SLRWRTRRRNRSLDERESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYF 180
            L WRTRRR+RSLDER++SG   +PP+ NQ RT IV+ P DQ +P+QP+IP  ANPRDYF
Sbjct: 121 WLHWRTRRRSRSLDERDNSG--RQPPNPNQSRTVIVLGPPDQFQPIQPIIPRRANPRDYF 180

Query: 181 AGPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEA 240
            GPRLD+LIE+LTQNDRPGPPPA + AIE IPTVKI AEHLKNDS CPVCKEEFEVGGEA
Sbjct: 181 TGPRLDELIEDLTQNDRPGPPPAGEEAIERIPTVKIAAEHLKNDSQCPVCKEEFEVGGEA 240

Query: 241 RELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWW 300
           REL+CKH+YHS+CIVPWLRLHNSCPVCRQE+PPLPP  EN   G+S++G  IGR C RWW
Sbjct: 241 RELSCKHLYHSECIVPWLRLHNSCPVCRQEIPPLPP--ENGAAGNSENGVRIGRACTRWW 300

Query: 301 GQLVSRPFRNRYRQITPHDQEN 320
            +L S  FRNRYRQI+P    N
Sbjct: 301 NRLASWLFRNRYRQISPSSPPN 314

[Close]

BLAST of MC11g0690 vs. ExPASy TrEMBL
Match: A0A1S4DVY7 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103487574 PE=4 SV=1)

HSP 1 Score: 446 bits (1147), Expect = 7.09e-154
Identity = 231/366 (63.11%), Postives = 275/366 (75.14%), Query Frame = 0


Query: 1   MSLSPPRATTNP-NIRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPTQ 60
           MSL+PP++TTN  +  TF LYWC+QCHR + LA+AH+S+L CPRCFG F+EE+ +  P +
Sbjct: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTITLASAHTSELICPRCFGQFIEEIQLTLP-E 60

Query: 61  LDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDDS 120
            +  PEARL EALSLML+ P+RL N R P+     R  P    F++FDRR   D EGD+ 
Sbjct: 61  FNPLPEARLFEALSLMLNQPIRLFNNRTPNGN---RHHPPLHRFEEFDRRSFSDPEGDEL 120

Query: 121 LRWRTRRRNRSLDERESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFA 180
            +WR R R+RSLDER++   G +PP+ NQ RT IV  P DQ +P+QP++P   +PRDYF 
Sbjct: 121 PQWRRRWRSRSLDERDN--FGPQPPNPNQSRTVIVFGPPDQLQPIQPILPRRVSPRDYFT 180

Query: 181 GPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEAR 240
           GP+LD+LIEELTQNDRPGP PAS+ AIE IP VKI AEHLKNDSHCPVCKEEFEVGGEAR
Sbjct: 181 GPQLDELIEELTQNDRPGPAPASEEAIERIPMVKIAAEHLKNDSHCPVCKEEFEVGGEAR 240

Query: 241 ELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWWG 300
           EL+CKHIYHS+CIVPWLRLHNSCPVCRQEMP L   PEN    SS++ EG+GRRCARWW 
Sbjct: 241 ELSCKHIYHSECIVPWLRLHNSCPVCRQEMPSL--TPENEASSSSEE-EGMGRRCARWWS 300

Query: 301 QLVSRPFRNRYRQITPHDQENIPPRGILFHSPEVNSWGGSIYLSSLALLMSMFMLQVTWF 360
           QLV  PFRNRYRQI+P  +           S E+NS GGS YL+S ALLMSMFMLQ+TW 
Sbjct: 301 QLVPWPFRNRYRQISPFPRNRFDAS-----SREINSRGGSSYLASSALLMSMFMLQITWI 352

Query: 361 LLEPLR 365
           LLE LR
Sbjct: 361 LLETLR 352

[Close]

BLAST of MC11g0690 vs. ExPASy TrEMBL
Match: A0A0A0L0L2 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G370570 PE=4 SV=1)

HSP 1 Score: 446 bits (1146), Expect = 1.01e-153
Identity = 229/366 (62.57%), Postives = 274/366 (74.86%), Query Frame = 0


Query: 1   MSLSPPRATTNP-NIRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPTQ 60
           MSL+PP++TTN  +  TF LYWC+QCHR V LA+AH S+LTCPRCFG F+EE+ +  P +
Sbjct: 1   MSLTPPQSTTNNRHTTTFQLYWCHQCHRTVTLASAHPSELTCPRCFGQFIEEVQLTLP-E 60

Query: 61  LDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDDS 120
            + SPE RL EALSLML+ P+R+ N R P+     R  P W  F++FDRR   D EGD+ 
Sbjct: 61  FNPSPEGRLFEALSLMLNQPIRIFNNRTPNGN---RHHPPWHRFEEFDRRSFSDPEGDEL 120

Query: 121 LRWRTRRRNRSLDERESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFA 180
            +WR R R+RSLDER++   G +PP+ N+ RT IV  P DQ +P+QP++P   +PRDYF 
Sbjct: 121 PQWRRRWRSRSLDERDN--FGQQPPNPNRSRTVIVFGPPDQLQPIQPILPRRISPRDYFT 180

Query: 181 GPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEAR 240
           GP+LD+LIEELTQNDRPGP PAS+ AIE IPTVKI AEHLKN+SHCPVCKEEFEVGGEAR
Sbjct: 181 GPQLDELIEELTQNDRPGPAPASEEAIERIPTVKIEAEHLKNESHCPVCKEEFEVGGEAR 240

Query: 241 ELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWWG 300
           EL+CKHIYHS+CIVPWLRLHNSCPVCRQEMP     PEN    SS++ EG+GRRCARWW 
Sbjct: 241 ELSCKHIYHSECIVPWLRLHNSCPVCRQEMPSF--TPENEASSSSEE-EGMGRRCARWWS 300

Query: 301 QLVSRPFRNRYRQITPHDQENIPPRGILFHSPEVNSWGGSIYLSSLALLMSMFMLQVTWF 360
            LV  PFRNRYRQI+P  +           S E+NS GGS YLSS ALLMSMFMLQ+TW 
Sbjct: 301 HLVPWPFRNRYRQISPFPRNRFDAS-----SREINSRGGSSYLSSSALLMSMFMLQLTWI 352

Query: 361 LLEPLR 365
           +LE  R
Sbjct: 361 ILETFR 352

[Close]

BLAST of MC11g0690 vs. ExPASy TrEMBL
Match: A0A6J1FTR5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111446755 PE=4 SV=1)

HSP 1 Score: 427 bits (1098), Expect = 5.54e-147
Identity = 219/326 (67.18%), Postives = 252/326 (77.30%), Query Frame = 0


Query: 1   MSLSPPRATTNPNIRTFDLYWCYQCHRAVRLAAAHS-SDLTCPRCFGHFLEELHVPRPTQ 60
           MS +  R T +    +F  YWC+QCHR V LA     S+L C RC G FLEELH+ RP Q
Sbjct: 1   MSFASSRHTRS----SFHFYWCHQCHRMVTLATTRDPSELICSRCSGQFLEELHLTRP-Q 60

Query: 61  LDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGDDS 120
            D SPE RLLEALSLMLDPPVRL  FRLPD +   RRRP+   FD+FDR+   D EGD+ 
Sbjct: 61  FDNSPETRLLEALSLMLDPPVRL--FRLPDID---RRRPMLHQFDNFDRQSFSDPEGDEW 120

Query: 121 LRWRTRRRNRSLDERESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFA 180
             WRTRRRNRSLDER++S   L+PP++N+ RT I++RP D SRP+QP+IPP A+PRDYF 
Sbjct: 121 FHWRTRRRNRSLDERDNSS--LQPPNQNRSRTVIILRPTDPSRPVQPIIPPRADPRDYFT 180

Query: 181 GPRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEHLKNDSHCPVCKEEFEVGGEAR 240
           GPRLD+LIEELTQNDRPGP PAS+AAIE IPTVKI AEHLKND+ CPVCKEEFE+G EAR
Sbjct: 181 GPRLDELIEELTQNDRPGPQPASEAAIERIPTVKIAAEHLKNDTMCPVCKEEFEIGEEAR 240

Query: 241 ELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIGRRCARWWG 300
           EL CKH+YHS+CIVPWLRLHNSCPVCRQEMPP PP  EN+   SS D EGIGRRC RWWG
Sbjct: 241 ELMCKHVYHSECIVPWLRLHNSCPVCRQEMPPSPP--ENQAAESSDDEEGIGRRCTRWWG 300

Query: 301 QLVSRPFRNRYRQITP-HDQENIPPR 324
           QLV+RPFR+RYRQ++P  D  N   R
Sbjct: 301 QLVNRPFRSRYRQLSPPQDSHNSSSR 312

[Close]

BLAST of MC11g0690 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 239.2 bits (609), Expect = 5.2e-63
Identity = 147/347 (42.36%), Postives = 193/347 (55.62%), Query Frame = 0


Query: 1   MSLSPP--RATTNPN--IRTFDLYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHV-- 60
           MSLS P  R  + PN   RTF LYWCY C R VR+A+++ S++ CPRC   F+ E+    
Sbjct: 1   MSLSQPITRTDSAPNGAFRTFGLYWCYHCDRMVRIASSNPSEIACPRCLRQFVVEIETRQ 60

Query: 61  -PRPT------QLDLSPEARLLEALSLMLDPPVRLVNFRLPDPEHEPRRRPLWQF-FDDF 120
            PR T        D SPEARLLEALSLM +P                    + +F  D F
Sbjct: 61  RPRFTFNHATPPFDASPEARLLEALSLMFEPAT------------------IGRFGADPF 120

Query: 121 DRRPLRDLEGDDSLRWRTRRRNRSLDERESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQP 180
            R   R++   +S      RR  SLD   + GL L        RT++++RP + + P+  
Sbjct: 121 LRARSRNILEPESRPRPQHRRRHSLDNVNNGGLPL------PRRTYVILRPNNPTSPLGN 180

Query: 181 LIPP-------GANPRDYFAG-PRLDDLIEELTQNDRPGPPPASDAAIEGIPTVKIVAEH 240
           +I P         N  DYF G   L+ LIE+LTQ+DRPGPPPAS+  I  +P+VKI  +H
Sbjct: 181 IIAPPNQAPPRHVNSHDYFTGASSLEQLIEQLTQDDRPGPPPASEPTINSLPSVKITPQH 240

Query: 241 LKND-SHCPVCKEEFEVGGEARELNCKHIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPE 300
           L ND S C VC EEF VGG+A EL CKHIYH DCIVPWLRL+NSCP+CR+++P +    E
Sbjct: 241 LTNDMSQCTVCMEEFIVGGDATELPCKHIYHKDCIVPWLRLNNSCPICRRDLPLVNTVAE 300

Query: 301 NRGPGSSQDGEGIGRRCARWWGQLVSRPFRNRYRQITPHDQENIPPR 325
           +R   +    +   RR  RW       PFR RY++++P +  N  PR
Sbjct: 301 SRERSNPIRQDMPERRRPRWMQLGNIWPFRARYQRVSPEETANQNPR 323

[Close]

BLAST of MC11g0690 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 158.7 bits (400), Expect = 9.0e-39
Identity = 99/280 (35.36%), Postives = 146/280 (52.14%), Query Frame = 0


Query: 19  LYWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPTQ-LDLSPEARLLEALSLMLD 78
           +++CYQC++ V ++ + S+D  CP C   FLEE   P P Q L+ +P +   ++   M D
Sbjct: 20  MFFCYQCNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSS--DSFFPMAD 79

Query: 79  PPVRLVNFRLPDPEHEPRRRPLWQFFDDFDRRPLRDLEGD---DSLRWRTRRRNRSLDER 138
           P   L+          P        F    +   R  + +   D+    T  +N  L   
Sbjct: 80  PFSTLLPLIFGSSAAAPSGMDFMSLFGPSMQPQARSTQQNPQSDAFDPFTFLQNH-LQTL 139

Query: 139 ESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFAGPRLDDLIEELTQND 198
            SSG   E         F++     ++ P  P      N  DYF GP L+ LI++L +ND
Sbjct: 140 RSSGTHFE---------FVI-----ENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAEND 199

Query: 199 --RPGPPPASDAAIEGIPTVKIVAEHLKND-SHCPVCKEEFEVGGEARELNCKHIYHSDC 258
             R G PPAS +AI+ +PTVK+  + LK++ + C VC +EFE G + +++ CKH++H DC
Sbjct: 200 PNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDC 259

Query: 259 IVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQDGEGIG 292
           ++PWL LHNSCPVCR E+P   P  ENR  GS   G+G G
Sbjct: 260 LLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSGDGQG 282

[Close]

BLAST of MC11g0690 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 152.9 bits (385), Expect = 4.9e-37
Identity = 102/282 (36.17%), Postives = 140/282 (49.65%), Query Frame = 0


Query: 20  YWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPTQLDLSPEAR----------LL 79
           +WC++C RAVRL   H  +  C  C G F+EEL + + +  D+    R          L+
Sbjct: 8   HWCHRCQRAVRL---HGQEPVCFYCGGGFVEELDMAQASPFDMFRSHRGVVERDQTFDLM 67

Query: 80  EALSLMLDPPVRLVNFRLPDPEH--EPRRRPLWQFFDDF-DRRPLRDLEGDDSLRWRTRR 139
           +A S+ +         RL +  H  E R R +    ++F    PL    G    R     
Sbjct: 68  DAFSVFM-------RNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDN 127

Query: 140 RNRSLDERESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFAGPRLDDL 199
              +L    S G+G+                               N  DYF GP L++L
Sbjct: 128 AVEALFNGGSPGIGI----------------------------TRGNTGDYFFGPGLEEL 187

Query: 200 IEELTQ-NDRPGPPPASDAAIEGIPTVKIVAEHLK-NDSHCPVCKEEFEVGGEARELNCK 259
            E+L+    R GPPPA  +AI+ +PT+KI   HL+ +DS+CPVCK+EFE+G EA+++ C 
Sbjct: 188 FEQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCN 245

Query: 260 HIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQD 287
           HIYHSDCIVPWL  HNSCPVCRQE+      P   GP SSQ+
Sbjct: 248 HIYHSDCIVPWLVQHNSCPVCRQEL------PSASGPSSSQN 245

[Close]

BLAST of MC11g0690 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 152.9 bits (385), Expect = 4.9e-37
Identity = 102/282 (36.17%), Postives = 140/282 (49.65%), Query Frame = 0


Query: 20  YWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPTQLDLSPEAR----------LL 79
           +WC++C RAVRL   H  +  C  C G F+EEL + + +  D+    R          L+
Sbjct: 8   HWCHRCQRAVRL---HGQEPVCFYCGGGFVEELDMAQASPFDMFRSHRGVVERDQTFDLM 67

Query: 80  EALSLMLDPPVRLVNFRLPDPEH--EPRRRPLWQFFDDF-DRRPLRDLEGDDSLRWRTRR 139
           +A S+ +         RL +  H  E R R +    ++F    PL    G    R     
Sbjct: 68  DAFSVFM-------RNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDN 127

Query: 140 RNRSLDERESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFAGPRLDDL 199
              +L    S G+G+                               N  DYF GP L++L
Sbjct: 128 AVEALFNGGSPGIGI----------------------------TRGNTGDYFFGPGLEEL 187

Query: 200 IEELTQ-NDRPGPPPASDAAIEGIPTVKIVAEHLK-NDSHCPVCKEEFEVGGEARELNCK 259
            E+L+    R GPPPA  +AI+ +PT+KI   HL+ +DS+CPVCK+EFE+G EA+++ C 
Sbjct: 188 FEQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCN 245

Query: 260 HIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQD 287
           HIYHSDCIVPWL  HNSCPVCRQE+      P   GP SSQ+
Sbjct: 248 HIYHSDCIVPWLVQHNSCPVCRQEL------PSASGPSSSQN 245

[Close]

BLAST of MC11g0690 vs. TAIR 10
Match: AT2G40830.3 (RING-H2 finger C1A )

HSP 1 Score: 152.9 bits (385), Expect = 4.9e-37
Identity = 102/282 (36.17%), Postives = 140/282 (49.65%), Query Frame = 0


Query: 20  YWCYQCHRAVRLAAAHSSDLTCPRCFGHFLEELHVPRPTQLDLSPEAR----------LL 79
           +WC++C RAVRL   H  +  C  C G F+EEL + + +  D+    R          L+
Sbjct: 8   HWCHRCQRAVRL---HGQEPVCFYCGGGFVEELDMAQASPFDMFRSHRGVVERDQTFDLM 67

Query: 80  EALSLMLDPPVRLVNFRLPDPEH--EPRRRPLWQFFDDF-DRRPLRDLEGDDSLRWRTRR 139
           +A S+ +         RL +  H  E R R +    ++F    PL    G    R     
Sbjct: 68  DAFSVFM-------RNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDN 127

Query: 140 RNRSLDERESSGLGLEPPSRNQPRTFIVIRPIDQSRPMQPLIPPGANPRDYFAGPRLDDL 199
              +L    S G+G+                               N  DYF GP L++L
Sbjct: 128 AVEALFNGGSPGIGI----------------------------TRGNTGDYFFGPGLEEL 187

Query: 200 IEELTQ-NDRPGPPPASDAAIEGIPTVKIVAEHLK-NDSHCPVCKEEFEVGGEARELNCK 259
            E+L+    R GPPPA  +AI+ +PT+KI   HL+ +DS+CPVCK+EFE+G EA+++ C 
Sbjct: 188 FEQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCN 245

Query: 260 HIYHSDCIVPWLRLHNSCPVCRQEMPPLPPAPENRGPGSSQD 287
           HIYHSDCIVPWL  HNSCPVCRQE+      P   GP SSQ+
Sbjct: 248 HIYHSDCIVPWLVQHNSCPVCRQEL------PSASGPSSSQN 245

The following BLAST results are available for this feature:

BLAST of MC11g0690 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LPN7	1.3e-37	35.36	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
O22197	6.9e-36	36.17	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
P0CH30	4.5e-35	35.69	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q94AK4	1.1e-33	36.21	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]
Q940T5	8.5e-26	30.23	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]

BLAST of MC11g0690 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7024775.1	9.16e-174	69.32	E3 ubiquitin-protein ligase RZF1, partial [Cucurbita argyrosperma subsp. argyros...	[more]
XP_023535798.1	2.78e-170	68.41	E3 ubiquitin-protein ligase RZF1-like [Cucurbita pepo subsp. pepo]	[more]
XP_038898007.1	9.27e-164	67.49	E3 ubiquitin-protein ligase RZF1 isoform X1 [Benincasa hispida]	[more]
KAG6591901.1	7.69e-159	70.50	E3 ubiquitin-protein ligase RZF1, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022975873.1	4.42e-158	70.81	E3 ubiquitin-protein ligase RZF1-like [Cucurbita maxima]	[more]

BLAST of MC11g0690 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IKH7	2.14e-158	70.81	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111476446 P...	[more]
A0A6J1FAA6	6.12e-158	70.19	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111443524...	[more]
A0A1S4DVY7	7.09e-154	63.11	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103487574 PE=4 ...	[more]
A0A0A0L0L2	1.01e-153	62.57	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G370570 PE...	[more]
A0A6J1FTR5	5.54e-147	67.18	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111446755...	[more]

BLAST of MC11g0690 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G60360.1	5.2e-63	42.36	RING/U-box superfamily protein	[more]
AT3G19950.1	9.0e-39	35.36	RING/U-box superfamily protein	[more]
AT2G40830.2	4.9e-37	36.17	RING-H2 finger C1A	[more]
AT2G40830.1	4.9e-37	36.17	RING-H2 finger C1A	[more]
AT2G40830.3	4.9e-37	36.17	RING-H2 finger C1A	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 225..265 e-value: 3.7E-7 score: 39.8
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 224..266 e-value: 6.4E-11 score: 42.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 225..266 score: 12.061441
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 188..272 e-value: 8.9E-22 score: 78.6
IPR039525	E3 ubiquitin-protein ligase RNF126-like, zinc-ribbon	PFAM	PF14369	zinc_ribbon_9	coord: 19..52 e-value: 3.2E-13 score: 49.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 129..150
None	No IPR available	PANTHER	PTHR15710:SF29	SUBFAMILY NOT NAMED	coord: 11..97 coord: 149..283
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 11..97
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 149..283
None	No IPR available	CDD	cd16667	RING-H2_RNF126_like	coord: 225..266 e-value: 1.60878E-22 score: 86.988
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 205..273

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g0690.1	MC11g0690.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0061630	ubiquitin protein ligase activity