MC11g0662 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC11g0662
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionMethyltransf_11 domain-containing protein
LocationMC11: 5291527 .. 5295245 (+)
RNA-Seq ExpressionMC11g0662
SyntenyMC11g0662
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCGGAACCAGATTGAGTCCAGAACTTCAAAGTTTATTCCCCCGCCCCCGCCCATGCTTTCGAAGACTACTGCAATCCAACCCAATGCGAACAATTGTCTATCACGCAGTAGTGCCATCCGTCCATAGCGAAGCGAAGCTTAGTGGTGGTGGAAGCTGATTCACTCTCAGAAAACCAAGTATCTGTGTCAGATCGCCGCGCCGGGGCATGGCTGCAGTCGTTGCAAAGCACATGTTGTTGGCCTTCGTCCCCACTCGACTCTGTTTCTTCAGGCGCTGCTGCTTCTTCAACTTCGATTCACCCACCCTATCCATTTCTACACGAATTTCCGCTTCAACCGTTCCAGCCTCTTCGATTGCCTCTATGGACACTAGGCTTCAGCCATCGGTTTGTATTCTCTTCTTTTTCCTGAAGTCGGTAACGTCCTCTGGTTTGTTTTTGAGAGAAACGCAAAGAAAAGAGAGGATATGAATTGGACCGAGTTATACTGAGGTGAGGATTTCAGTGAACAAAACATTATCAGCTCTTCAAGGTTTGAGAACATTGTTTTCTGGTAGTGAGAGAGGATATGTAGTTATAAACTGTTGCTGTGCTGTACATTGTTCGAATTGAATTAAATCTTTATGTACCCCCGGTGAGAAGTAAGATGTTAAGAATACTGTTTGGAACATGATTGAATTTATGTGTCGCGTAAGAGTGATAAAAGTATACTGGAAAAAGGTAAATTGACCTAACCTATCAACTTAATTAGTTTTGTTCGTTTCGTTTTTCTTTTATCTAATAATTTCCGGTTCTTGTATAGGACTCTTCTATACTTGACAGAGAAGTCAACAACATTGAAAATATTTTAGCTTGTTCCGTATGTTATGGCCCATTGACTGCGGCGGCGGCTGGTTCGGGCCTGTCAGTGTATGTTAGGAACTGCGCCTATTTTTACTTTTCTTATTTCTTCTATTTATAATATTTACTTAAATGCTTTTATACAGAGAGTCCACAAATGGATATCAATTGGAATGTGGCACATGTAGAAAGACTTTCACTGGTAGTGGAACTCATTTGGACTTGACGATAGCCAGTGGAAATAACAATTATGGTGATCCCATGCCTGCTTCCACGGAGATTTTTAGGTAATATGATATTTATTGTTACTAAGACTTTCAAATGCAAAAGACTTTCAAATCTTTATGCTCTATATTAGAAGGCACTCTTGTTCAAAAATAGAAAACTTGATTGCGCCTGGTTGAAATTATGTAATCTCTGGTGTTGAGAAGCTGTGAGCCGCCAATCTATAGTACTTACCTTGACGCCTCCGAAAATGGAAATAATAAAGTTACTTGTACTATGATTTGAATTTTCTTATGGTTTCATTTGTCCTTTTTTGTTTATCGTAATCAGTATTTGTAGAAGTCCCTTTACATCCAGAATGACGAAACAGATGCTGATATTATCATTTAGTGGTTTAACGCATTATTAAATACCAAATTAATATGTCACTTCTGTTGGCATACATAATCGGGAAGTATACCATTCCCTACATATCCAATACAATAGGACATATTGATCAACCGTACCTGGGATTTGCATAGGTTTGGGCTATTTTCTAAATTATTTTAATTCCTTGCTATGTGGAATTAGAATATTACTGGTAGCCTTTAGTTGTATTGAGAAAAATGAACTTTCGGGTGTTGGGTTGACATTCTTGTCATTTCTCTTCTTCCCCTTTGTTTCTCCTCTCATTTTTATCAGCTTTTTGTTGGATCAGGACTAGATTGGTGTCTTTTCTTTATGAGAGGGGCTGGCGTCAAAGCTTTAGTGTAGCCCTTGGTTTTCCTGGCCCAGAGACGGAGGTGATGTTAGCTCTTTTATCTCTCTTCAATATAGAATTGTTCACATGGTACTTTTTACTTAATGTCAGTTGGTGAGTTATAGGTTCGATATTAGGAGATGGATCAGTCCATTAATACCAATTGAAAATGCTAAGTCAGAGAGAGACAGAGTTTAGTGATTTCTTATCAGAACGCGTCTCTCTTTTCATGTTTACAATGGTGATGTTGACAGTTTTCATTCAACACATGGTTGTGAATGCTGAGCACGTGATGATTTCGTTGCAGTTTGAGTTGATCAGAACTTTCATGACACCGATCTTAGGTGGAAACATAATTGATGCAAGTTGTGGAAGTGGATTGTTTTCAAGAATCTTTGCCAAGAGTGGGCTATTTTCGTCTGTTGTTGCTTTGGACTACTCGGAGAACATGTTGAAGCAGTGCTATGAATTCATTCAACAAGAGGAAAATTTCCCCAGAGAGTAAATAGTATTTCCTTGTAGTTTCTTATTACATTATTTGGCGGTAGTTCAATTGCTATAGTTCAAGGATCTTTCTGCCCTATATTTATATTGTTGTTTGAGTTTTTACATCTAAACAGATTCTTCTTATTCGTAGGAATTTGGTTCTGATCAGAGCTGATATTGCTCGGCTTCCATTTGCATCAAATTCTGTTGATGCAGTGCATGCCGGTGCTGCTTTGCACTGCTGGCCTTCACCATCAGCTGCTGTGAGTAGCTTTTCATCTATACCTGGGGCTTATAGCTCCTCGGCTTGTAATCAACTTTTTATAATTGCACTAATTGAAAACACACTGGACGAGCTGCAGTTTATTAATGGCTAATTGCCTTTTTACTAGAGTGATATGATTATTCTCATCTTCAAAGCAAATCTAACTTTGCTTCTGTGTATGGGTGTGAAATGTGTGTATCTGCCAGCACGTGCTGATGTGTAACATTACCTATCACTATAATTGTACCATAGATCCATCCTACCAAAATAATCAATGGGTAGGCTTTTAGTTATTAACTATGTGCCATGTGGATGTGGACTTGCAGGTGGCTGAAATAAGTAGAATTTTAAGACCTGGAGGAGTGTTTGTTGCCAGCACCTTCATAGCCGATGGGCCGTTTTCTTTTATACCATTTTTGGGGACTCAAAGAGAGGTATGTTCGCAATTATTTTAATTTCACCTCTAAAACTTCTGGGAATTTCGTAGATTGCTTGCTTAGTGAATTATATCTTACTTTTACACGCTGCTTATCCTATTTGGATTGTCAATCAAAACAATCACCTCTACTCAAGTTGTCAACAATTGCTTTATGCTGTGAGATTCATATTACTTCTGTAATATAACTCTGATTTGTTCTTCTTACTCCTATAATATCACACCAACAGCGTGTAAAGCAGATAACAGGGAGCCACATCGTATTATCTGAACGTGAATTAGAAGAACTTTGCAGGGCCTGTGGTCTAGTCGGCTTTAGGTGTGTAAGAAATAGGCTCTTTGTAATGATCTCTGCTACAAAACCCAGCTAAACATTTGGTTTTCTGGTGGTTCCCAATCCTTCTCCCGCAGTCATTTTGGTTTATTAAAAAAGTTCAAAAGTTGGTGGTTAATGTTGTCTGCCACACTAATGCGCCGGTGTTTGGCTGCTGGCTGCCCTTCATCTGTTTGTAACATAATATATCTAAATCTTTTAGCTTCTGTTTCTTTATAGCCTCTACTAGATTATTTAGTGCGGAGAGAAAAAATTAAGATAAATTCCAACGCAAAAGGAGTGTGAACAACTTAAGAAGGGCTTGAGCTCCTGACATTAGAGTTATTAGAATCATTTTGTAAACAACTGAGCTATTCCAGCATTTTGGTACTAAAATTCGTTAATAATAACTAAC

mRNA sequence

CCCGGAACCAGATTGAGTCCAGAACTTCAAAGTTTATTCCCCCGCCCCCGCCCATGCTTTCGAAGACTACTGCAATCCAACCCAATGCGAACAATTGTCTATCACGCAGTAGTGCCATCCGTCCATAGCGAAGCGAAGCTTAGTGGTGGTGGAAGCTGATTCACTCTCAGAAAACCAAGTATCTGTGTCAGATCGCCGCGCCGGGGCATGGCTGCAGTCGTTGCAAAGCACATGTTGTTGGCCTTCGTCCCCACTCGACTCTGTTTCTTCAGGCGCTGCTGCTTCTTCAACTTCGATTCACCCACCCTATCCATTTCTACACGAATTTCCGCTTCAACCGTTCCAGCCTCTTCGATTGCCTCTATGGACACTAGGCTTCAGCCATCGGACTCTTCTATACTTGACAGAGAAGTCAACAACATTGAAAATATTTTAGCTTGTTCCGTATGTTATGGCCCATTGACTGCGGCGGCGGCTGGTTCGGGCCTGTCAGTAGAGTCCACAAATGGATATCAATTGGAATGTGGCACATGTAGAAAGACTTTCACTGGTAGTGGAACTCATTTGGACTTGACGATAGCCAGTGGAAATAACAATTATGGTGATCCCATGCCTGCTTCCACGGAGATTTTTAGGACTAGATTGGTGTCTTTTCTTTATGAGAGGGGCTGGCGTCAAAGCTTTAGTGTAGCCCTTGGTTTTCCTGGCCCAGAGACGGAGTTTGAGTTGATCAGAACTTTCATGACACCGATCTTAGGTGGAAACATAATTGATGCAAGTTGTGGAAGTGGATTGTTTTCAAGAATCTTTGCCAAGAGTGGGCTATTTTCGTCTGTTGTTGCTTTGGACTACTCGGAGAACATGTTGAAGCAGTGCTATGAATTCATTCAACAAGAGGAAAATTTCCCCAGAGAGAATTTGGTTCTGATCAGAGCTGATATTGCTCGGCTTCCATTTGCATCAAATTCTGTTGATGCAGTGCATGCCGGTGCTGCTTTGCACTGCTGGCCTTCACCATCAGCTGCTGTGGCTGAAATAAGTAGAATTTTAAGACCTGGAGGAGTGTTTGTTGCCAGCACCTTCATAGCCGATGGGCCGTTTTCTTTTATACCATTTTTGGGGACTCAAAGAGAGCGTGTAAAGCAGATAACAGGGAGCCACATCGTATTATCTGAACGTGAATTAGAAGAACTTTGCAGGGCCTGTGGTCTAGTCGGCTTTAGGTGTGTAAGAAATAGGCTCTTTGTAATGATCTCTGCTACAAAACCCAGCTAAACATTTGGTTTTCTGGTGGTTCCCAATCCTTCTCCCGCAGTCATTTTGGTTTATTAAAAAAGTTCAAAAGTTGGTGGTTAATGTTGTCTGCCACACTAATGCGCCGGTGTTTGGCTGCTGGCTGCCCTTCATCTGTTTGTAACATAATATATCTAAATCTTTTAGCTTCTGTTTCTTTATAGCCTCTACTAGATTATTTAGTGCGGAGAGAAAAAATTAAGATAAATTCCAACGCAAAAGGAGTGTGAACAACTTAAGAAGGGCTTGAGCTCCTGACATTAGAGTTATTAGAATCATTTTGTAAACAACTGAGCTATTCCAGCATTTTGGTACTAAAATTCGTTAATAATAACTAAC

Coding sequence (CDS)

ATGGCTGCAGTCGTTGCAAAGCACATGTTGTTGGCCTTCGTCCCCACTCGACTCTGTTTCTTCAGGCGCTGCTGCTTCTTCAACTTCGATTCACCCACCCTATCCATTTCTACACGAATTTCCGCTTCAACCGTTCCAGCCTCTTCGATTGCCTCTATGGACACTAGGCTTCAGCCATCGGACTCTTCTATACTTGACAGAGAAGTCAACAACATTGAAAATATTTTAGCTTGTTCCGTATGTTATGGCCCATTGACTGCGGCGGCGGCTGGTTCGGGCCTGTCAGTAGAGTCCACAAATGGATATCAATTGGAATGTGGCACATGTAGAAAGACTTTCACTGGTAGTGGAACTCATTTGGACTTGACGATAGCCAGTGGAAATAACAATTATGGTGATCCCATGCCTGCTTCCACGGAGATTTTTAGGACTAGATTGGTGTCTTTTCTTTATGAGAGGGGCTGGCGTCAAAGCTTTAGTGTAGCCCTTGGTTTTCCTGGCCCAGAGACGGAGTTTGAGTTGATCAGAACTTTCATGACACCGATCTTAGGTGGAAACATAATTGATGCAAGTTGTGGAAGTGGATTGTTTTCAAGAATCTTTGCCAAGAGTGGGCTATTTTCGTCTGTTGTTGCTTTGGACTACTCGGAGAACATGTTGAAGCAGTGCTATGAATTCATTCAACAAGAGGAAAATTTCCCCAGAGAGAATTTGGTTCTGATCAGAGCTGATATTGCTCGGCTTCCATTTGCATCAAATTCTGTTGATGCAGTGCATGCCGGTGCTGCTTTGCACTGCTGGCCTTCACCATCAGCTGCTGTGGCTGAAATAAGTAGAATTTTAAGACCTGGAGGAGTGTTTGTTGCCAGCACCTTCATAGCCGATGGGCCGTTTTCTTTTATACCATTTTTGGGGACTCAAAGAGAGCGTGTAAAGCAGATAACAGGGAGCCACATCGTATTATCTGAACGTGAATTAGAAGAACTTTGCAGGGCCTGTGGTCTAGTCGGCTTTAGGTGTGTAAGAAATAGGCTCTTTGTAATGATCTCTGCTACAAAACCCAGCTAA

Protein sequence

MAAVVAKHMLLAFVPTRLCFFRRCCFFNFDSPTLSISTRISASTVPASSIASMDTRLQPSDSSILDREVNNIENILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGTHLDLTIASGNNNYGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTFMTPILGGNIIDASCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRENLVLIRADIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPFSFIPFLGTQRERVKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKPS
Homology
BLAST of MC11g0662 vs. ExPASy Swiss-Prot
Match: Q8LBV4 (Uncharacterized methyltransferase At1g78140, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g78140 PE=1 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 1.6e-109
Identity = 192/319 (60.19%), Postives = 250/319 (78.37%), Query Frame = 0

Query: 40  ISASTVPASSIASMDTRLQPSDSSILDREVNN--IENILACSVCYGPLTAAAAGSGLSVE 99
           +S S   +S+  S++T    +   +++++  N   + ILAC +CY  L   +  +GL   
Sbjct: 38  VSRSAHASSASVSVETNSNSNVDFVIEKKDKNRGEKKILACPICYNSLAWISQPNGLIES 97

Query: 100 STNGYQLECGTCRKTFTGSGTHLDLTIASGNNNYGDPMPASTEIFRTRLVSFLYERGWRQ 159
           + +G Q++C TC+++++G+ THLDL +ASG+  Y +PMP STE+FRT LVSFLYERGWRQ
Sbjct: 98  AASGIQVQCNTCKRSYSGNETHLDLAVASGSKRYSEPMPLSTELFRTPLVSFLYERGWRQ 157

Query: 160 SFSVALGFPGPETEFELIRTFMTPILGGNIIDASCGSGLFSRIFAKSGLFSSVVALDYSE 219
           +F +  GFPGPE EFE+ + ++ P+LGGNIIDASCGSG+FSR+F +S LFS V+ALDYSE
Sbjct: 158 NF-IWGGFPGPEKEFEMAKAYLKPVLGGNIIDASCGSGMFSRLFTRSDLFSLVIALDYSE 217

Query: 220 NMLKQCYEFIQQEENFP-RENLVLIRADIARLPFASNSVDAVHAGAALHCWPSPSAAVAE 279
           NML+QCYE + +EENFP +E LVL+RADIARLPF S SVDAVHAGAALHCWPSPS+AVAE
Sbjct: 218 NMLRQCYELLNKEENFPNKEKLVLVRADIARLPFLSGSVDAVHAGAALHCWPSPSSAVAE 277

Query: 280 ISRILRPGGVFVASTFIADGPFSFIPFLGTQRERVKQITGSHIVLSERELEELCRACGLV 339
           ISR+LRPGGVFVA+TFI DGPFSFIPFL   R+ + + +GSHI L+ERELE++C+ACGLV
Sbjct: 278 ISRVLRPGGVFVATTFIYDGPFSFIPFLKNLRQEIMRYSGSHIFLNERELEDICKACGLV 337

Query: 340 GFRCVRNRLFVMISATKPS 356
            F  VRN  F+M+SATKPS
Sbjct: 338 NFTRVRNGPFIMLSATKPS 355

BLAST of MC11g0662 vs. ExPASy Swiss-Prot
Match: Q0WPT7 (Uncharacterized methyltransferase At2g41040, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g41040 PE=1 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 2.2e-71
Identity = 152/347 (43.80%), Postives = 209/347 (60.23%), Query Frame = 0

Query: 13  FVPTRLCFFRRCCFFNFDSPTLSISTRISASTVPASSIASMDTRLQPSDSSILDREVNNI 72
           F+P R  F        F S + S   R SAST   S   S         S I   E   I
Sbjct: 20  FLPNRSSF--------FSSDSQSPLLRFSASTSVRSRFPSAAISAVAPKSDINKNETPKI 79

Query: 73  E----NILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGTHLDLTIASGN 132
           E     + AC VCY PL      SG+++++      +CG C KT++    +LDLT+ +  
Sbjct: 80  EIEETQVFACPVCYEPLMRKGP-SGINLQAIYRSGFKCGQCNKTYSSKDEYLDLTVTADL 139

Query: 133 NNYGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTFMTPILGGNII 192
           ++Y +  P +TE+FR+ LVSFLYERGWRQ+F  + GFPGP+ EF +   +     GG ++
Sbjct: 140 DDYNEVKPITTELFRSPLVSFLYERGWRQAFKRS-GFPGPDEEFRMAEEYFKEAEGGLLV 199

Query: 193 DASCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRE-NLVLIRADIAR 252
           D SCGSGLFSR FA+SG +S V+ALDYSENML+QC EFI+ +  F    N+ ++RAD++R
Sbjct: 200 DVSCGSGLFSRKFAQSGKYSGVIALDYSENMLRQCKEFIKNDNTFDNSTNIAVVRADVSR 259

Query: 253 LPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPFSFIPFLGTQ 312
           LPF S SVDAVHAGAALHCWPSP+ A+AEI R+LR GGVFV +TF+   P +  P++   
Sbjct: 260 LPFPSGSVDAVHAGAALHCWPSPTNAIAEICRVLRSGGVFVGTTFLRYSPST--PWI--I 319

Query: 313 RERVKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKP 355
           R    +I  S+  L + E++++C +CGL  +       F+M +A KP
Sbjct: 320 RPFQSRILQSYNYLMQDEIKDVCTSCGLTDYEDYIQDSFIMFTARKP 352

BLAST of MC11g0662 vs. ExPASy Swiss-Prot
Match: Q2YY85 (Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain bovine RF122 / ET3-1) OX=273036 GN=menG PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.2e-08
Identity = 37/106 (34.91%), Postives = 57/106 (53.77%), Query Frame = 0

Query: 184 GGNIIDASCGSGLFSRIFAKS-GLFSSVVALDYSENMLKQCYEFIQQEENFPRENLVLIR 243
           G   +D  CG+G ++   +K+ G    V  +D+SENML+     + +E+    EN+ L+ 
Sbjct: 50  GTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLE-----VGKEKTASMENVKLVH 109

Query: 244 ADIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFV 289
            D   LPF  NS D V  G  L   P    A+ E++R+L+PGG+ V
Sbjct: 110 GDAMELPFGDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVV 150

BLAST of MC11g0662 vs. ExPASy Swiss-Prot
Match: A7X2H6 (Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain Mu3 / ATCC 700698) OX=418127 GN=menG PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.6e-08
Identity = 37/106 (34.91%), Postives = 57/106 (53.77%), Query Frame = 0

Query: 184 GGNIIDASCGSGLFSRIFAKS-GLFSSVVALDYSENMLKQCYEFIQQEENFPRENLVLIR 243
           G   +D  CG+G ++   +K+ G    V  +D+SENML+     + +E+    EN+ L+ 
Sbjct: 50  GTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLE-----VGKEKTASMENVKLVH 109

Query: 244 ADIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFV 289
            D   LPF  NS D V  G  L   P    A+ E++R+L+PGG+ V
Sbjct: 110 GDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVV 150

BLAST of MC11g0662 vs. ExPASy Swiss-Prot
Match: A6U1T9 (Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain JH1) OX=359787 GN=menG PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.6e-08
Identity = 37/106 (34.91%), Postives = 57/106 (53.77%), Query Frame = 0

Query: 184 GGNIIDASCGSGLFSRIFAKS-GLFSSVVALDYSENMLKQCYEFIQQEENFPRENLVLIR 243
           G   +D  CG+G ++   +K+ G    V  +D+SENML+     + +E+    EN+ L+ 
Sbjct: 50  GTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLE-----VGKEKTASMENVKLVH 109

Query: 244 ADIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFV 289
            D   LPF  NS D V  G  L   P    A+ E++R+L+PGG+ V
Sbjct: 110 GDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVV 150

BLAST of MC11g0662 vs. NCBI nr
Match: XP_022147083.1 (uncharacterized methyltransferase At1g78140, chloroplastic [Momordica charantia])

HSP 1 Score: 703 bits (1814), Expect = 3.12e-255
Identity = 355/355 (100.00%), Postives = 355/355 (100.00%), Query Frame = 0

Query: 1   MAAVVAKHMLLAFVPTRLCFFRRCCFFNFDSPTLSISTRISASTVPASSIASMDTRLQPS 60
           MAAVVAKHMLLAFVPTRLCFFRRCCFFNFDSPTLSISTRISASTVPASSIASMDTRLQPS
Sbjct: 1   MAAVVAKHMLLAFVPTRLCFFRRCCFFNFDSPTLSISTRISASTVPASSIASMDTRLQPS 60

Query: 61  DSSILDREVNNIENILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGTHL 120
           DSSILDREVNNIENILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGTHL
Sbjct: 61  DSSILDREVNNIENILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGTHL 120

Query: 121 DLTIASGNNNYGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTFMT 180
           DLTIASGNNNYGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTFMT
Sbjct: 121 DLTIASGNNNYGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTFMT 180

Query: 181 PILGGNIIDASCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRENLVL 240
           PILGGNIIDASCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRENLVL
Sbjct: 181 PILGGNIIDASCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRENLVL 240

Query: 241 IRADIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPFSF 300
           IRADIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPFSF
Sbjct: 241 IRADIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPFSF 300

Query: 301 IPFLGTQRERVKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKPS 355
           IPFLGTQRERVKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKPS
Sbjct: 301 IPFLGTQRERVKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKPS 355

BLAST of MC11g0662 vs. NCBI nr
Match: XP_038896626.1 (uncharacterized methyltransferase At1g78140, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 533 bits (1374), Expect = 3.33e-188
Identity = 282/356 (79.21%), Postives = 313/356 (87.92%), Query Frame = 0

Query: 3   AVVAKHMLLAF--VPTRLCFFRRCCFFNFDSPTLSISTRISASTVPASSIASMDTRLQ-P 62
           ++VA HM  AF  +PTR CFFRRC FF+F+S  LSIS   S S V +S  ASM+T  Q P
Sbjct: 5   SMVANHMY-AFLPIPTRSCFFRRC-FFSFNS-NLSISPPTSLSIVRSSLAASMETTTQQP 64

Query: 63  SDSSILDREVNNIENILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGTH 122
           SD  ++D+EVNNI+NILACS+CYGPLTAA   SGLSVESTNGYQLECGTC+K+FTGS +H
Sbjct: 65  SDLVMIDKEVNNIKNILACSICYGPLTAAV-DSGLSVESTNGYQLECGTCKKSFTGSESH 124

Query: 123 LDLTIASGNNNYGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTFM 182
           LDLTIASG  N G+ MPA+TEIFRTRLVSFLYERGWRQSFS  LGFPGPE EFEL++ FM
Sbjct: 125 LDLTIASGIKN-GESMPAATEIFRTRLVSFLYERGWRQSFSPFLGFPGPEKEFELLKNFM 184

Query: 183 TPILGGNIIDASCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRENLV 242
           TPILGGNIIDASCGSG+FSRIFAKSGLFSSVVALDYSENMLK+CYEFI+QEENFP+E+LV
Sbjct: 185 TPILGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYEFIKQEENFPKESLV 244

Query: 243 LIRADIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPFS 302
           LIRADIARLPFAS+SVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFI DGPFS
Sbjct: 245 LIRADIARLPFASSSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIIDGPFS 304

Query: 303 FIPFLGTQRERVKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKPS 355
           FIPFLG Q E ++QITGSHIVLSE EL+ELC ACGLV F+C+RNR FVM+SATK S
Sbjct: 305 FIPFLGIQIEGIQQITGSHIVLSEGELKELCTACGLVDFKCLRNRRFVMLSATKRS 355

BLAST of MC11g0662 vs. NCBI nr
Match: XP_022937076.1 (uncharacterized methyltransferase At1g78140, chloroplastic [Cucurbita moschata])

HSP 1 Score: 530 bits (1366), Expect = 4.93e-187
Identity = 277/357 (77.59%), Postives = 310/357 (86.83%), Query Frame = 0

Query: 1   MAAVVAKHMLLAFVP--TRLCFFRRCCFFNFDSPTLSISTRISASTVPASSIASMDTRLQ 60
           MAA++ KHM + FVP  TR CF RRC FF FDS  LSIS R S STV A+S ASM+TR +
Sbjct: 1   MAALLGKHMFV-FVPFPTRPCFPRRC-FFEFDS-ALSISHRTSVSTVRATSAASMETRTE 60

Query: 61  PSDSSILDREVNNIENILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGT 120
            SD  ++D+EVNNIENILACSVCYGPLTAAA GSGLSVEST GYQLECGTC+K+FTGS +
Sbjct: 61  LSDPVLIDKEVNNIENILACSVCYGPLTAAA-GSGLSVESTKGYQLECGTCKKSFTGSES 120

Query: 121 HLDLTIASGNNNYGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTF 180
           HLDLTI++G NN  + MPA+TEIFRTRLVSFLYERGWRQSFS  LGFPGPE EFE I+ F
Sbjct: 121 HLDLTISAGINNE-ESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNF 180

Query: 181 MTPILGGNIIDASCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRENL 240
           +TP+LGGNIIDASCGSG+FSRIFAKSGLFSSVVALDYSENMLK+CY FI++EENFP+E+L
Sbjct: 181 ITPVLGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESL 240

Query: 241 VLIRADIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPF 300
           VLIRADIARLPFAS+S+DAVHAGAALHCWPSPSAAVAEISRIL+PGGVFV ST+I DGPF
Sbjct: 241 VLIRADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPF 300

Query: 301 SFIPFLGTQRERVKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKPS 355
           SF+PFLGTQ E  K+  G HIVLSERELEELC ACG+V FRC+RNR FVM SA K S
Sbjct: 301 SFMPFLGTQIEIAKKTIGGHIVLSERELEELCTACGMVDFRCLRNRRFVMFSARKRS 352

BLAST of MC11g0662 vs. NCBI nr
Match: XP_023535731.1 (uncharacterized methyltransferase At1g78140, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 530 bits (1365), Expect = 6.99e-187
Identity = 277/357 (77.59%), Postives = 310/357 (86.83%), Query Frame = 0

Query: 1   MAAVVAKHMLLAFVP--TRLCFFRRCCFFNFDSPTLSISTRISASTVPASSIASMDTRLQ 60
           MAA++ KHM + FVP  TR CF RRC FF FDS  LSIS R S STV A+S ASM+TR +
Sbjct: 1   MAALLGKHMFV-FVPFPTRPCFPRRC-FFKFDS-ALSISHRASVSTVRATSAASMETRTE 60

Query: 61  PSDSSILDREVNNIENILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGT 120
            SD  ++D+EVNNIENILACSVCYGPLTAAA GSGLSVEST GYQLECGTC+K+FTGS +
Sbjct: 61  LSDPVLIDKEVNNIENILACSVCYGPLTAAA-GSGLSVESTKGYQLECGTCKKSFTGSES 120

Query: 121 HLDLTIASGNNNYGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTF 180
           HLDLTI++G NN  + MPA+TEIFRTRLVSFLYERGWRQSFS  LGFPGPE EFE I+ F
Sbjct: 121 HLDLTISAGINNE-ESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNF 180

Query: 181 MTPILGGNIIDASCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRENL 240
           +TP+LGGNIIDASCGSG+FSRIFAKSGLFSSVVALDYSENMLK+CY FI++EENFP+E+L
Sbjct: 181 ITPVLGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESL 240

Query: 241 VLIRADIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPF 300
           VLIRADIARLPFAS+S+DAVHAGAALHCWPSPSAAVAEISRIL+PGGVFV ST+I DGPF
Sbjct: 241 VLIRADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPF 300

Query: 301 SFIPFLGTQRERVKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKPS 355
           SF+PFLGTQ E  K+I G HIVLSE ELEELC ACG+V FRC+RNR FVM SA K S
Sbjct: 301 SFMPFLGTQIEIAKKIIGGHIVLSESELEELCMACGMVDFRCLRNRRFVMFSARKRS 352

BLAST of MC11g0662 vs. NCBI nr
Match: KAG7024628.1 (putative methyltransferase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 530 bits (1364), Expect = 9.93e-187
Identity = 277/357 (77.59%), Postives = 309/357 (86.55%), Query Frame = 0

Query: 1   MAAVVAKHMLLAFVP--TRLCFFRRCCFFNFDSPTLSISTRISASTVPASSIASMDTRLQ 60
           MAA++ KHM + FVP  TR CF RRC FF FDS  LSIS R S STV A+S ASM+TR +
Sbjct: 1   MAALLGKHMFV-FVPFPTRPCFPRRC-FFKFDS-ALSISHRTSVSTVRATSAASMETRTE 60

Query: 61  PSDSSILDREVNNIENILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGT 120
            SD  ++D+EVNNIENILACSVCYGPLTAAA GSGLSVEST GYQLECGTC+K+FTGS +
Sbjct: 61  LSDPVLIDKEVNNIENILACSVCYGPLTAAA-GSGLSVESTKGYQLECGTCKKSFTGSES 120

Query: 121 HLDLTIASGNNNYGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTF 180
           HLDLTI++G NN  + MP +TEIFRTRLVSFLYERGWRQSFS  LGFPGPE EFE I+ F
Sbjct: 121 HLDLTISAGINNE-ESMPVTTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNF 180

Query: 181 MTPILGGNIIDASCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRENL 240
           +TP+LGGNIIDASCGSG+FSRIFAKSGLFSSVVALDYSENMLK+CY FI++EENFP+E+L
Sbjct: 181 ITPVLGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESL 240

Query: 241 VLIRADIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPF 300
           VLIRADIARLPFAS+S+DAVHAGAALHCWPSPSAAVAEISRIL+PGGVFV ST+I DGPF
Sbjct: 241 VLIRADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPF 300

Query: 301 SFIPFLGTQRERVKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKPS 355
           SF+PFLGTQ E  K+  G HIVLSERELEELC ACGLV FRC+RNR FVM SA K S
Sbjct: 301 SFMPFLGTQIEVAKKTIGGHIVLSERELEELCTACGLVDFRCLRNRRFVMFSARKRS 352

BLAST of MC11g0662 vs. ExPASy TrEMBL
Match: A0A6J1D0A7 (uncharacterized methyltransferase At1g78140, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111016077 PE=4 SV=1)

HSP 1 Score: 703 bits (1814), Expect = 1.51e-255
Identity = 355/355 (100.00%), Postives = 355/355 (100.00%), Query Frame = 0

Query: 1   MAAVVAKHMLLAFVPTRLCFFRRCCFFNFDSPTLSISTRISASTVPASSIASMDTRLQPS 60
           MAAVVAKHMLLAFVPTRLCFFRRCCFFNFDSPTLSISTRISASTVPASSIASMDTRLQPS
Sbjct: 1   MAAVVAKHMLLAFVPTRLCFFRRCCFFNFDSPTLSISTRISASTVPASSIASMDTRLQPS 60

Query: 61  DSSILDREVNNIENILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGTHL 120
           DSSILDREVNNIENILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGTHL
Sbjct: 61  DSSILDREVNNIENILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGTHL 120

Query: 121 DLTIASGNNNYGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTFMT 180
           DLTIASGNNNYGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTFMT
Sbjct: 121 DLTIASGNNNYGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTFMT 180

Query: 181 PILGGNIIDASCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRENLVL 240
           PILGGNIIDASCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRENLVL
Sbjct: 181 PILGGNIIDASCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRENLVL 240

Query: 241 IRADIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPFSF 300
           IRADIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPFSF
Sbjct: 241 IRADIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPFSF 300

Query: 301 IPFLGTQRERVKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKPS 355
           IPFLGTQRERVKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKPS
Sbjct: 301 IPFLGTQRERVKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKPS 355

BLAST of MC11g0662 vs. ExPASy TrEMBL
Match: A0A6J1F9C0 (uncharacterized methyltransferase At1g78140, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111443486 PE=4 SV=1)

HSP 1 Score: 530 bits (1366), Expect = 2.39e-187
Identity = 277/357 (77.59%), Postives = 310/357 (86.83%), Query Frame = 0

Query: 1   MAAVVAKHMLLAFVP--TRLCFFRRCCFFNFDSPTLSISTRISASTVPASSIASMDTRLQ 60
           MAA++ KHM + FVP  TR CF RRC FF FDS  LSIS R S STV A+S ASM+TR +
Sbjct: 1   MAALLGKHMFV-FVPFPTRPCFPRRC-FFEFDS-ALSISHRTSVSTVRATSAASMETRTE 60

Query: 61  PSDSSILDREVNNIENILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGT 120
            SD  ++D+EVNNIENILACSVCYGPLTAAA GSGLSVEST GYQLECGTC+K+FTGS +
Sbjct: 61  LSDPVLIDKEVNNIENILACSVCYGPLTAAA-GSGLSVESTKGYQLECGTCKKSFTGSES 120

Query: 121 HLDLTIASGNNNYGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTF 180
           HLDLTI++G NN  + MPA+TEIFRTRLVSFLYERGWRQSFS  LGFPGPE EFE I+ F
Sbjct: 121 HLDLTISAGINNE-ESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNF 180

Query: 181 MTPILGGNIIDASCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRENL 240
           +TP+LGGNIIDASCGSG+FSRIFAKSGLFSSVVALDYSENMLK+CY FI++EENFP+E+L
Sbjct: 181 ITPVLGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESL 240

Query: 241 VLIRADIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPF 300
           VLIRADIARLPFAS+S+DAVHAGAALHCWPSPSAAVAEISRIL+PGGVFV ST+I DGPF
Sbjct: 241 VLIRADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPF 300

Query: 301 SFIPFLGTQRERVKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKPS 355
           SF+PFLGTQ E  K+  G HIVLSERELEELC ACG+V FRC+RNR FVM SA K S
Sbjct: 301 SFMPFLGTQIEIAKKTIGGHIVLSERELEELCTACGMVDFRCLRNRRFVMFSARKRS 352

BLAST of MC11g0662 vs. ExPASy TrEMBL
Match: A0A6J1IKY5 (uncharacterized methyltransferase At1g78140, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476562 PE=4 SV=1)

HSP 1 Score: 527 bits (1357), Expect = 5.59e-186
Identity = 275/357 (77.03%), Postives = 309/357 (86.55%), Query Frame = 0

Query: 1   MAAVVAKHMLLAFVP--TRLCFFRRCCFFNFDSPTLSISTRISASTVPASSIASMDTRLQ 60
           MAA++ KHM + FVP  TR CF  + CFF FDS  LSIS R S STV A+S  SM+TR +
Sbjct: 1   MAALLGKHMFV-FVPFPTRPCF-PQPCFFKFDS-ALSISHRASVSTVRATSATSMETRTE 60

Query: 61  PSDSSILDREVNNIENILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGT 120
            SD  ++D+EVNNIENILACSVCYGPLTAAA GSGLSVEST GYQLECGTC+K+FTGS +
Sbjct: 61  LSDPVLIDKEVNNIENILACSVCYGPLTAAA-GSGLSVESTKGYQLECGTCKKSFTGSES 120

Query: 121 HLDLTIASGNNNYGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTF 180
           HLDLTI++G NN  + MPA+TEIFRTRLVSFLYERGWRQSFS  LGFPGPE EFE I+ F
Sbjct: 121 HLDLTISAGINNE-ESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNF 180

Query: 181 MTPILGGNIIDASCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRENL 240
           +TP+LGGNIIDASCGSG+FSRIFAKSGLFSSVVALDYSENMLK+CY FI++EENFP+E+L
Sbjct: 181 ITPVLGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESL 240

Query: 241 VLIRADIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPF 300
           VLIRADIARLPFAS+S+DAVHAGAALHCWPSPSAAVAEISRIL+PGGVFV ST+I DGPF
Sbjct: 241 VLIRADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPF 300

Query: 301 SFIPFLGTQRERVKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKPS 355
           SF+PFLGTQ E  K+I G HIVLSERELEELC ACG+V FRC+RNR FVM SA K S
Sbjct: 301 SFMPFLGTQIEIAKKIIGGHIVLSERELEELCTACGMVDFRCLRNRRFVMFSARKRS 352

BLAST of MC11g0662 vs. ExPASy TrEMBL
Match: A0A1S3CQA4 (uncharacterized methyltransferase At1g78140, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503586 PE=4 SV=1)

HSP 1 Score: 510 bits (1314), Expect = 2.26e-179
Identity = 265/345 (76.81%), Postives = 298/345 (86.38%), Query Frame = 0

Query: 14  VPTRLCFFRRCCFFNFDSPTLSISTRISASTVPASSIASMDTR---LQPSDSSILDREVN 73
           +PT+   F RC FFNF S TLSIS R+S ST+ +SS   M+TR   + PSDS ++D+EVN
Sbjct: 16  LPTQFSSFTRC-FFNFHS-TLSISPRLSLSTLRSSSAPPMETRTNIIPPSDSVMIDKEVN 75

Query: 74  NIENILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGTHLDLTIASGNNN 133
            I+NILACS+C GPLTAAA  SGLSVESTNGYQLECGTC+K+FTGS +HLDLTI  G N+
Sbjct: 76  KIKNILACSICRGPLTAAA-DSGLSVESTNGYQLECGTCKKSFTGSESHLDLTITGGINS 135

Query: 134 YGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTFMTPILGGNIIDA 193
            G+ MPA+TEIFRTRLVSFLYERGWRQSFSV LGFPGPE EFELI+ F+TP+LGG+IIDA
Sbjct: 136 -GESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFITPVLGGSIIDA 195

Query: 194 SCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRENLVLIRADIARLPF 253
           SCGSG+FSRIFAKSGLFSSVVALDYSENML+QCY FI+QEENFP E LVLIRADIARLPF
Sbjct: 196 SCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYGFIKQEENFPNERLVLIRADIARLPF 255

Query: 254 ASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPFSFIPFLGTQRER 313
           AS+SVDAVHAGAA+HCWPSPSA VAEISRILRPGGVFVASTFI DGPFSF+PFL  Q E 
Sbjct: 256 ASSSVDAVHAGAAVHCWPSPSAGVAEISRILRPGGVFVASTFIMDGPFSFVPFLRIQIEG 315

Query: 314 VKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKPS 355
           ++QI GS I LSERELEELC ACGLV F+C+RNR FVM+SATK S
Sbjct: 316 IQQIAGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKRS 356

BLAST of MC11g0662 vs. ExPASy TrEMBL
Match: A0A7J6HJQ9 (Methyltransf_11 domain-containing protein OS=Cannabis sativa OX=3483 GN=F8388_019472 PE=4 SV=1)

HSP 1 Score: 449 bits (1156), Expect = 2.66e-155
Identity = 217/292 (74.32%), Postives = 250/292 (85.62%), Query Frame = 0

Query: 66  DREVNNIE--NILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGTHLDLT 125
           D ++NN +  NILAC +CY PL       GLSVES +G  L+CGTC+KT+ G+ THL+LT
Sbjct: 68  DEDINNKKRSNILACPICYEPLALTGGELGLSVESVSGSSLQCGTCKKTYFGNETHLELT 127

Query: 126 IASGNNNYGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTFMTPIL 185
            ASG  NYG+PMPASTEIFRT LVSFLYERGWRQSFSV  GFPGPE EF+L++ F+ P+L
Sbjct: 128 AASGTKNYGEPMPASTEIFRTPLVSFLYERGWRQSFSVLGGFPGPEKEFDLVQKFIKPVL 187

Query: 186 GGNIIDASCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRENLVLIRA 245
           GGNIIDASCGSGLFSR+FAKS LFS VVALDYSENMLKQCYEFIQQEENFP+EN+VL+RA
Sbjct: 188 GGNIIDASCGSGLFSRLFAKSNLFSLVVALDYSENMLKQCYEFIQQEENFPKENIVLVRA 247

Query: 246 DIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPFSFIPF 305
           DI+RLPFAS+S+DAVHAGAALHCWPSP +AVAEISR+LRPGGVFVAST+I DGPFSF PF
Sbjct: 248 DISRLPFASSSIDAVHAGAALHCWPSPLSAVAEISRVLRPGGVFVASTYILDGPFSFFPF 307

Query: 306 LGTQRERVKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKPS 355
           L   R+  +QI+GS+I LS+RELE+LCRA GLVGF CVRN LFVMISA KP+
Sbjct: 308 LRNIRQNTRQISGSYIFLSQRELEDLCRASGLVGFTCVRNGLFVMISAKKPN 359

BLAST of MC11g0662 vs. TAIR 10
Match: AT1G78140.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 397.5 bits (1020), Expect = 1.1e-110
Identity = 192/319 (60.19%), Postives = 250/319 (78.37%), Query Frame = 0

Query: 40  ISASTVPASSIASMDTRLQPSDSSILDREVNN--IENILACSVCYGPLTAAAAGSGLSVE 99
           +S S   +S+  S++T    +   +++++  N   + ILAC +CY  L   +  +GL   
Sbjct: 38  VSRSAHASSASVSVETNSNSNVDFVIEKKDKNRGEKKILACPICYNSLAWISQPNGLIES 97

Query: 100 STNGYQLECGTCRKTFTGSGTHLDLTIASGNNNYGDPMPASTEIFRTRLVSFLYERGWRQ 159
           + +G Q++C TC+++++G+ THLDL +ASG+  Y +PMP STE+FRT LVSFLYERGWRQ
Sbjct: 98  AASGIQVQCNTCKRSYSGNETHLDLAVASGSKRYSEPMPLSTELFRTPLVSFLYERGWRQ 157

Query: 160 SFSVALGFPGPETEFELIRTFMTPILGGNIIDASCGSGLFSRIFAKSGLFSSVVALDYSE 219
           +F +  GFPGPE EFE+ + ++ P+LGGNIIDASCGSG+FSR+F +S LFS V+ALDYSE
Sbjct: 158 NF-IWGGFPGPEKEFEMAKAYLKPVLGGNIIDASCGSGMFSRLFTRSDLFSLVIALDYSE 217

Query: 220 NMLKQCYEFIQQEENFP-RENLVLIRADIARLPFASNSVDAVHAGAALHCWPSPSAAVAE 279
           NML+QCYE + +EENFP +E LVL+RADIARLPF S SVDAVHAGAALHCWPSPS+AVAE
Sbjct: 218 NMLRQCYELLNKEENFPNKEKLVLVRADIARLPFLSGSVDAVHAGAALHCWPSPSSAVAE 277

Query: 280 ISRILRPGGVFVASTFIADGPFSFIPFLGTQRERVKQITGSHIVLSERELEELCRACGLV 339
           ISR+LRPGGVFVA+TFI DGPFSFIPFL   R+ + + +GSHI L+ERELE++C+ACGLV
Sbjct: 278 ISRVLRPGGVFVATTFIYDGPFSFIPFLKNLRQEIMRYSGSHIFLNERELEDICKACGLV 337

Query: 340 GFRCVRNRLFVMISATKPS 356
            F  VRN  F+M+SATKPS
Sbjct: 338 NFTRVRNGPFIMLSATKPS 355

BLAST of MC11g0662 vs. TAIR 10
Match: AT2G41040.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 270.8 bits (691), Expect = 1.6e-72
Identity = 152/347 (43.80%), Postives = 209/347 (60.23%), Query Frame = 0

Query: 13  FVPTRLCFFRRCCFFNFDSPTLSISTRISASTVPASSIASMDTRLQPSDSSILDREVNNI 72
           F+P R  F        F S + S   R SAST   S   S         S I   E   I
Sbjct: 20  FLPNRSSF--------FSSDSQSPLLRFSASTSVRSRFPSAAISAVAPKSDINKNETPKI 79

Query: 73  E----NILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGTHLDLTIASGN 132
           E     + AC VCY PL      SG+++++      +CG C KT++    +LDLT+ +  
Sbjct: 80  EIEETQVFACPVCYEPLMRKGP-SGINLQAIYRSGFKCGQCNKTYSSKDEYLDLTVTADL 139

Query: 133 NNYGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTFMTPILGGNII 192
           ++Y +  P +TE+FR+ LVSFLYERGWRQ+F  + GFPGP+ EF +   +     GG ++
Sbjct: 140 DDYNEVKPITTELFRSPLVSFLYERGWRQAFKRS-GFPGPDEEFRMAEEYFKEAEGGLLV 199

Query: 193 DASCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRE-NLVLIRADIAR 252
           D SCGSGLFSR FA+SG +S V+ALDYSENML+QC EFI+ +  F    N+ ++RAD++R
Sbjct: 200 DVSCGSGLFSRKFAQSGKYSGVIALDYSENMLRQCKEFIKNDNTFDNSTNIAVVRADVSR 259

Query: 253 LPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPFSFIPFLGTQ 312
           LPF S SVDAVHAGAALHCWPSP+ A+AEI R+LR GGVFV +TF+   P +  P++   
Sbjct: 260 LPFPSGSVDAVHAGAALHCWPSPTNAIAEICRVLRSGGVFVGTTFLRYSPST--PWI--I 319

Query: 313 RERVKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKP 355
           R    +I  S+  L + E++++C +CGL  +       F+M +A KP
Sbjct: 320 RPFQSRILQSYNYLMQDEIKDVCTSCGLTDYEDYIQDSFIMFTARKP 352

BLAST of MC11g0662 vs. TAIR 10
Match: AT5G13710.1 (sterol methyltransferase 1 )

HSP 1 Score: 49.7 bits (117), Expect = 5.7e-06
Identity = 46/155 (29.68%), Postives = 67/155 (43.23%), Query Frame = 0

Query: 146 LVSFLYERGWRQSFSVALGFPGPETEFELIR--TFMTPIL----GGNIIDASCGSGLFSR 205
           L +  YE GW +SF  A  + G      + R   F+   L    G  ++D  CG G   R
Sbjct: 51  LATSFYEYGWGESFHFAQRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLR 110

Query: 206 IFAKSGLFSSVVALDYSENMLKQCYEF-----IQQEENFPRENLVLIRADIARLPFASNS 265
             A+    S V  L+ +E  + +  E      + +  NF       ++AD  ++PF  NS
Sbjct: 111 EIARFS-NSVVTGLNNNEYQITRGKELNRLAGVDKTCNF-------VKADFMKMPFPENS 170

Query: 266 VDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVA 290
            DAV+A  A    P       EI R+L+PG  F A
Sbjct: 171 FDAVYAIEATCHAPDAYGCYKEIYRVLKPGQCFAA 197

BLAST of MC11g0662 vs. TAIR 10
Match: AT5G13710.2 (sterol methyltransferase 1 )

HSP 1 Score: 49.7 bits (117), Expect = 5.7e-06
Identity = 46/155 (29.68%), Postives = 67/155 (43.23%), Query Frame = 0

Query: 146 LVSFLYERGWRQSFSVALGFPGPETEFELIR--TFMTPIL----GGNIIDASCGSGLFSR 205
           L +  YE GW +SF  A  + G      + R   F+   L    G  ++D  CG G   R
Sbjct: 51  LATSFYEYGWGESFHFAQRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLR 110

Query: 206 IFAKSGLFSSVVALDYSENMLKQCYEF-----IQQEENFPRENLVLIRADIARLPFASNS 265
             A+    S V  L+ +E  + +  E      + +  NF       ++AD  ++PF  NS
Sbjct: 111 EIARFS-NSVVTGLNNNEYQITRGKELNRLAGVDKTCNF-------VKADFMKMPFPENS 170

Query: 266 VDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVA 290
            DAV+A  A    P       EI R+L+PG  F A
Sbjct: 171 FDAVYAIEATCHAPDAYGCYKEIYRVLKPGQCFAA 197

BLAST of MC11g0662 vs. TAIR 10
Match: AT1G20330.1 (sterol methyltransferase 2 )

HSP 1 Score: 48.5 bits (114), Expect = 1.3e-05
Identity = 49/208 (23.56%), Postives = 84/208 (40.38%), Query Frame = 0

Query: 146 LVSFLYERGWRQSFSVALGFPG------PETEFELIRTFMTPILGGNIIDASCGSGLFSR 205
           LV+ +YE GW QSF  +   PG           E+    +    G  I+D  CG G   R
Sbjct: 80  LVTDIYEWGWGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQVKPGQKILDVGCGVGGPMR 139

Query: 206 IFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRENLVLIRADIARLPFASNSVDAVH 265
             A S   ++VV +  +E  + +    +  ++        ++  +  ++PF  NS D  +
Sbjct: 140 AIA-SHSRANVVGITINEYQVNRAR--LHNKKAGLDALCEVVCGNFLQMPFDDNSFDGAY 199

Query: 266 AGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPFSFIPFLGTQRERVKQITG--- 325
           +  A    P      AEI R+L+PG ++V+  ++    F          E V+ I G   
Sbjct: 200 SIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFK-----AEDDEHVEVIQGIER 259

Query: 326 SHIVLSERELEELCRACGLVGFRCVRNR 345
              +   R   ++      VGF  V+ +
Sbjct: 260 GDALPGLRAYVDIAETAKKVGFEIVKEK 279

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LBV41.6e-10960.19Uncharacterized methyltransferase At1g78140, chloroplastic OS=Arabidopsis thalia... [more]
Q0WPT72.2e-7143.80Uncharacterized methyltransferase At2g41040, chloroplastic OS=Arabidopsis thalia... [more]
Q2YY851.2e-0834.91Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain bovine RF... [more]
A7X2H61.6e-0834.91Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain Mu3 / ATC... [more]
A6U1T91.6e-0834.91Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain JH1) OX=3... [more]
Match NameE-valueIdentityDescription
XP_022147083.13.12e-255100.00uncharacterized methyltransferase At1g78140, chloroplastic [Momordica charantia][more]
XP_038896626.13.33e-18879.21uncharacterized methyltransferase At1g78140, chloroplastic isoform X1 [Benincasa... [more]
XP_022937076.14.93e-18777.59uncharacterized methyltransferase At1g78140, chloroplastic [Cucurbita moschata][more]
XP_023535731.16.99e-18777.59uncharacterized methyltransferase At1g78140, chloroplastic [Cucurbita pepo subsp... [more]
KAG7024628.19.93e-18777.59putative methyltransferase, chloroplastic [Cucurbita argyrosperma subsp. argyros... [more]
Match NameE-valueIdentityDescription
A0A6J1D0A71.51e-255100.00uncharacterized methyltransferase At1g78140, chloroplastic OS=Momordica charanti... [more]
A0A6J1F9C02.39e-18777.59uncharacterized methyltransferase At1g78140, chloroplastic OS=Cucurbita moschata... [more]
A0A6J1IKY55.59e-18677.03uncharacterized methyltransferase At1g78140, chloroplastic-like OS=Cucurbita max... [more]
A0A1S3CQA42.26e-17976.81uncharacterized methyltransferase At1g78140, chloroplastic OS=Cucumis melo OX=36... [more]
A0A7J6HJQ92.66e-15574.32Methyltransf_11 domain-containing protein OS=Cannabis sativa OX=3483 GN=F8388_01... [more]
Match NameE-valueIdentityDescription
AT1G78140.11.1e-11060.19S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT2G41040.11.6e-7243.80S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G13710.15.7e-0629.68sterol methyltransferase 1 [more]
AT5G13710.25.7e-0629.68sterol methyltransferase 1 [more]
AT1G20330.11.3e-0523.56sterol methyltransferase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 142..353
e-value: 1.1E-32
score: 115.3
NoneNo IPR availablePANTHERPTHR43591:SF46OS08G0411200 PROTEINcoord: 40..354
NoneNo IPR availablePANTHERPTHR43591METHYLTRANSFERASEcoord: 40..354
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 186..294
e-value: 7.48754E-15
score: 67.8402
IPR013216Methyltransferase type 11PFAMPF08241Methyltransf_11coord: 189..288
e-value: 1.6E-22
score: 80.0
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 149..345

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC11g0662.1MC11g0662.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0006744 ubiquinone biosynthetic process
molecular_function GO:0008168 methyltransferase activity