MC11g0525 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g0525 vs. ExPASy Swiss-Prot
Match: Q585H6 (Flagellar attachment zone protein 1 OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 GN=FAZ1 PE=4 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.2e-12
Identity = 179/690 (25.94%), Postives = 299/690 (43.33%), Query Frame = 0

Query: 377  SFSKDISNLQISEQNVLQMELD---NQHRKLTNDMSLVNTSLNEILERNQSLVDELSQCR 436
            S + D  N  +   N  Q E D    Q ++  ++   ++  L +++  NQSL ++L    
Sbjct: 896  SVTLDEYNDHMHRSNQFQQENDLLRQQLQQANDERENLHDRLEQLMAENQSLSEQLHNMH 955

Query: 437  SEL----RD---VSSAREELKNQLLTAEAEIENLSSRATESEKNLEKFHADMFRLSKELD 496
             EL    RD   V+   E L  ++    AE E L     +S  ++   +  + RL +EL+
Sbjct: 956  EELEREERDRSGVTLQNERLAEEIQRKTAENEQLVLENNKSRSDIRNLNVQVQRLMEELE 1015

Query: 497  DCKHLVTALEEENERLNGVITSENENKMKLAEEKELYINENEKISSELSSFKSLKVALED 556
                       ENE+L   +  +     KLAEE EL + ENEK++ EL     LKVA   
Sbjct: 1016 -------LKAAENEKLAEELELKAAENEKLAEELELKVAENEKLAEEL----ELKVA--- 1075

Query: 557  ENSKLIGSLSSVAEGKTKLEEEREQLFQVNGTLSVELENCRNLIATQQEEITNLIKNLAL 616
            EN KL   L   A    KL EE E     N  L+ ELE    L A + E+   L + L L
Sbjct: 1076 ENEKLAEELELKAAENEKLAEELELKAAENEKLAEELE----LKAAENEK---LAEELEL 1135

Query: 617  VTEDRTKLEEDKTLLFHENEKMASELLV-------LDERLSAEYGERVRFEDDLRDALVR 676
               +  KL E+  L   ENEK+A EL +       L E L  +  E  +  ++L   +  
Sbjct: 1136 KAAENEKLAEELELKAAENEKLAEELELKAAENEKLAEELELKAAENEKLAEELELKVAE 1195

Query: 677  LEKLTE-------ENIFLSNSLDIQKSKTEELCGEILPKQMRSIEDGNQTETADSGWHRV 736
             EKL E       EN  L+  L+++ ++ E+L  E+   ++++ E+    E  +      
Sbjct: 1196 NEKLAEELELKAAENEKLAEELELKVAENEKLAEEL---ELKAAENEKLAEELEL----- 1255

Query: 737  NKSQENDAYQIKKQELFDDSFEFITLGRHLEEAYLVLQKLEKEIKGLQSNSASFSRSGSK 796
             K+ EN+      +EL   + E   L   LE      +KL +E++   + +   +    +
Sbjct: 1256 -KAAENEKL---AEELELKAAENEKLAEELELKAAENEKLAEELELKVAENEKLAEE-LE 1315

Query: 797  VAAPAVSKLIEAFETKVNEEHEVESEIQL-PEDPYKLSSEL----VDNLGLLLHQVVVDS 856
            + A    KL E  E KV E  ++  E++L   +  KL+ EL     +N  L     +  +
Sbjct: 1316 LKAAENEKLAEELELKVAENEKLAEELELKAAENEKLAEELELKVAENEKLAEELELKAA 1375

Query: 857  ENASVLLKGERDHKKVAISTLNKLTDQFE--ALENH--SNDLVIANIDLGVLFEYLKHHV 916
            EN  +      +  ++ ++   KL ++ E  A EN   + +L +   +   L E L+   
Sbjct: 1376 ENEKL-----AEELELKVAENEKLAEELELKAAENEKLAEELELKAAENEKLAEELELKA 1435

Query: 917  DDAGGKIYELE---NLNESLKQQGLYHKISNCELAERLRGYDLKLTELQ--SQLCDLHQS 976
             +      ELE     NE L ++       N +LAE L   +LK+ E +  ++  +L  +
Sbjct: 1436 AENEKLAEELELKAAENEKLAEELELKAAENEKLAEEL---ELKVAENEKLAEELELKAA 1495

Query: 977  SNEMVSSTCN----QLDKLREGEIARATILEKDLQSFLLELAESIAKLDESLGKSDTAAL 1025
             NE ++        + +KL E    +A   EK  +   L+ AE+  KL E L       L
Sbjct: 1496 ENEKLAEELELKVAENEKLAEELELKAAENEKLAEELELKAAEN-EKLAEEL------EL 1536

BLAST of MC11g0525 vs. ExPASy Swiss-Prot
Match: P25386 (Intracellular protein transport protein USO1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=USO1 PE=1 SV=2)

HSP 1 Score: 71.2 bits (173), Expect = 1.9e-10
Identity = 214/1083 (19.76%), Postives = 471/1083 (43.49%), Query Frame = 0

Query: 1494 HKSLSEAFHDRDSLHQRVNYLENYCGLLTADLDDSQKKISDLEAELHSLVLEREKLSEKL 1553
            HK L E +   +S H  +   EN+  +L  +L + +  + ++  +L  ++  ++K ++  
Sbjct: 771  HKELDEKYQILNSSHSSLK--ENF-SILETELKNVRDSLDEM-TQLRDVLETKDKENQTA 830

Query: 1554 EIIYH---HNENLAFGNFENEVGIIVLQNE-----LSNMQENLISTEHKIVKLEALVSNA 1613
             + Y    H +  +    E  +  I+ Q +     ++ M ++L +   ++  +E    N 
Sbjct: 831  LLEYKSTIHKQEDSIKTLEKGLETILSQKKKAEDGINKMGKDLFALSREMQAVEENCKNL 890

Query: 1614 LQDEDVNDLVSGSSIEFLDLMVTKLIQNYTASSSGKAVLGKATNGHDTEEEMLARS-KNM 1673
             +++D +++      + L   +   I    A +     +    N    E+E +++     
Sbjct: 891  QKEKDKSNVNHQKETKSLKEDIAAKITEIKAINENLEEMKIQCNNLSKEKEHISKELVEY 950

Query: 1674 HDAWQNDINILKKELEDALNQLMVVTEERDQYMEMHESLVVKVESSDKKKDELQELLNLE 1733
               +Q+  N++ K L + L  L      +D   E +ESL+  VE S  K +   +L NL+
Sbjct: 951  KSRFQSHDNLVAK-LTEKLKSL--ANNYKDMQAE-NESLIKAVEES--KNESSIQLSNLQ 1010

Query: 1734 EQKSASIREKLNVAVRKGKSLIQQRDSLKQAVEEMTTELKHLRSEMKSQENTLASYEQNF 1793
             +  +  +EK N  + +G S+ +  + LK+ +    ++L+  + E+ S+ +  +S ++  
Sbjct: 1011 NKIDSMSQEKENFQIERG-SIEKNIEQLKKTI----SDLEQTKEEIISKSD--SSKDEYE 1070

Query: 1794 KDFSVYSGRAEALESENLSLKNRLAEIERNFQEKEHKLSSIINTLVHIEDNVDVSENDPI 1853
               S+   + E   + N    N+++E+ +  +E E +L++  N    +E  ++ SE    
Sbjct: 1071 SQISLLKEKLETATTANDENVNKISELTKTREELEAELAAYKNLKNELETKLETSE---- 1130

Query: 1854 EKLKQVGKLCSDLREAMYFSEQESVKSRRAAELLLAELNEVQERNDTFQEELAKASDEIA 1913
            + LK+V +    L+E     E+E+ ++++    L A L  +++ ++    +L K  ++IA
Sbjct: 1131 KALKEVKENEEHLKEEKIQLEKEATETKQQLNSLRANLESLEKEHEDLAAQLKKYEEQIA 1190

Query: 1914 ELTKERDLAETSKLEALSELEKLSTLHSKEKKIQYSQFMGLKSGLDRLK---EAMREINC 1973
               KER        E +S+L    T   +E +        +K   D L+   +AM+  + 
Sbjct: 1191 --NKERQYN-----EEISQLNDEITSTQQENE-------SIKKKNDELEGEVKAMKSTSE 1250

Query: 1974 LLADAFSKDLDAFYNLEAAIQSCTEPNGPADVNLSHSIVSGAFKSRDLKDKGKFFSLD-S 2033
              ++    ++DA       ++   E N  + +    S+ S   K ++L+D+  F   + S
Sbjct: 1251 EQSNLKKSEIDALNLQIKELKKKNETNEASLLESIKSVESETVKIKELQDECNFKEKEVS 1310

Query: 2034 WSNFYTNAHVDEN-----VATEIHSLVHQLEESMKEIGALKEMIDGHSVSFHKQSDSLSK 2093
                   A  D+N     +  E   +  +L+    E+    E I   S +  K    LS+
Sbjct: 1311 ELEDKLKASEDKNSKYLELQKESEKIKEELDAKTTELKIQLEKITNLSKAKEKSESELSR 1370

Query: 2094 ILGVLYREVNSQKELVQALKWDVQ-QSESVAKDREMEGDILCRNIAMLFEACTSVIKEVD 2153
            +      E  + +E ++ LK ++Q ++++  K+R+           +L E  +++ +E  
Sbjct: 1371 LKKTSSEERKNAEEQLEKLKNEIQIKNQAFEKERK-----------LLNEGSSTITQEYS 1430

Query: 2154 ER-------------KGELMGHDLTSGNLGMEIISTTPDQLSHAGKTHLLS-EESVRKIA 2213
            E+             + EL   ++ +    +E +S + D+L    +  + S ++ +    
Sbjct: 1431 EKINTLEDELIRLQNENELKAKEIDNTRSELEKVSLSNDELLEEKQNTIKSLQDEILSYK 1490

Query: 2214 DRL------LLAVREFIGFKAEMLDGSLKEMKVSVANLQKELQ--EKDIQKERICMELVG 2273
            D++      LL++        E L   L+  + S A +++ L+  E++  KE+  +E   
Sbjct: 1491 DKITRNDEKLLSIERDNKRDLESLKEQLRAAQESKAKVEEGLKKLEEESSKEKAELEKSK 1550

Query: 2274 Q-IKEAEATASRYSIDLQASKDQVYELEKVTEQMDSERKVLEQRLREMQ----DGLSISD 2333
            + +K+ E+T      +L++S + +    K  E+++  +K  E+ ++ +Q    D +S  +
Sbjct: 1551 EMMKKLESTIESNETELKSSMETI---RKSDEKLEQSKKSAEEDIKNLQHEKSDLISRIN 1610

Query: 2334 ELRERVRLLTGSL---AAKDQEIEALMHALD--EEELQMEG-----LTKKIEDLERDLKQ 2393
            E  + +  L   L   A    E+E +   L+  +E++++       L  K+ED+ER+LK 
Sbjct: 1611 ESEKDIEELKSKLRIEAKSGSELETVKQELNNAQEKIRINAEENTVLKSKLEDIERELKD 1670

Query: 2394 KNQELESIEASRGKLMKKLSITVTKFDELHHLSESLLTEVEKLQAQLQDRDAEISFLRQE 2453
            K  E++S +  +  L  +L            L + L +  +K Q   ++R AE+   + E
Sbjct: 1671 KQAEIKSNQEEKELLTSRLK----------ELEQELDSTQQKAQKSEEERRAEVRKFQVE 1730

Query: 2454 VTRCTNDALVATQTSNRNSEDINEVITWFDTMEARVGLSHSGHDDKENEVRECKEVLKKK 2506
             ++    A++      + ++ +N+   W                  E+ V++  +  +++
Sbjct: 1731 KSQLDEKAML---LETKYNDLVNKEQAW---------------KRDEDTVKKTTDSQRQE 1776

BLAST of MC11g0525 vs. ExPASy Swiss-Prot
Match: C9ZN16 (Flagellar attachment zone protein 1 OS=Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) OX=679716 GN=TbgDal_IV3690 PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 6.2e-09
Identity = 228/926 (24.62%), Postives = 389/926 (42.01%), Query Frame = 0

Query: 162  VDEQGRMVEVWSGCREEEISPARSASLLQAREDVGMTEDASMQSDQVCE--TQLAEDKQL 221
            ++ Q  ++   SG  EE+         L+A  ++   E+ S  S+Q     TQ  E+KQ 
Sbjct: 714  LNAQDDLLAELSGVSEEKEKLEAECERLEA--ELRQMEEKSRLSEQGLSEMTQRLEEKQA 773

Query: 222  QTGGMYESAAETTFKDTHCDEEEIITVDVASVSGFATV-SNDFSISNPGENLGMQSSSSS 281
            +  G+ E+  +         +E++  +  A  S  A + + D  IS+  + L  +     
Sbjct: 774  EIEGLLENLEQL--------DEQLEALRAAEKSAQAHIEARDREISDLQQRLEGEIDDHI 833

Query: 282  SRDDWKEERQVHAE------DMMHPSMYQVQYMPEDNFAVQS-EGHERPSQTSAK-ISEG 341
                  EE + H        D     +   +   ++   V+S E   RP  T  K   E 
Sbjct: 834  KTTALLEELRKHYNNLEELFDKQEAELMAYREKRQNAHKVRSLEPTLRPIGTQTKPFQEV 893

Query: 342  GDADAIFPNAHMTTDLAVQSGTFYSFGQESEFLDLL-ERVKEELIVTSFSKDISNLQISE 401
              AD I     ++  L   +   +   Q  +  DLL +++++         D     ++E
Sbjct: 894  VSADEISSEPLLSVTLDEYNDHMHRSNQFQQENDLLRQQLQQANDERENLHDRLEQLMAE 953

Query: 402  QNVLQMELDNQHRKLTND------MSLVNTSLNEILER----NQSLVDELSQCRSELRDV 461
               L  +L N H +L  +      ++L N  L E ++R    N+ LV E ++ RS++R++
Sbjct: 954  NQSLSEQLHNMHEELEREERDRSGVTLQNERLAEEIQRKTAENEQLVLENNKSRSDIRNL 1013

Query: 462  SSAREELKNQLLTAEAEIENLSS----RATESEK---NLEKFHADMFRLSKELDDCKHLV 521
            +   + L  +L    AE E L+     +A E+EK    LE   A+  +L++ LD      
Sbjct: 1014 NVQVQRLMEELELKAAENEKLAEELELKAAENEKLAEELELKAAENEKLAEALD------ 1073

Query: 522  TALEEENERLNGVITSENENKMKLAEEKELYINENEKISSELSSFKSLKVALEDENSKLI 581
                 ENE+L   +  +     KLAEE EL + ENEK++ EL     LK A   EN KL 
Sbjct: 1074 -LKAAENEKLAEELELKVAENEKLAEELELKVAENEKLAEEL----ELKAA---ENEKLA 1133

Query: 582  GSLSSVAEGKTKLEEEREQLFQVNGTLSVELENCRNLIATQQEEITNLIKNLALVTEDRT 641
              L   A    KL EE E     N  L+ ELE    L A + E+   L + L L   +  
Sbjct: 1134 EELELKAAENEKLAEELELKAAENEKLAEELE----LKAAENEK---LAEALDLKAAENE 1193

Query: 642  KLEEDKTLLFHENEKMASELLV-------LDERLSAEYGERVRFEDDLRDALVRLEKLTE 701
            KL E+  L   ENEK+A EL +       L E L  +  E  +  ++L       EKL E
Sbjct: 1194 KLAEELDLKAAENEKLAEELELKVAENEKLAEELELKAAENEKLAEELELKAAENEKLAE 1253

Query: 702  -------ENIFLSNSLDIQKSKTEELCGEILPKQMRSIEDGNQTETADSGWHRVNKSQEN 761
                   EN  L+  L+++ ++ E+L  E+  K   + +   + E   +   ++ +  E 
Sbjct: 1254 ELELKAAENEKLAEELELKVAENEKLAEELELKAAENEKLAEELELKAAENEKLAEELEL 1313

Query: 762  DAYQIKK--QELFDDSFEFITLGRHLEEAYLVLQKLEKEIKGLQSNSASFSRSGSKVAAP 821
             A + +K  +EL     E   L   LE      +KL +E++   + +   +    ++ A 
Sbjct: 1314 KAAENEKLAEELELKVAENEKLAEELELKAAENEKLAEELELKAAENEKLAEE-LELKAA 1373

Query: 822  AVSKLIEAFETKVNEEHEVESEIQL-PEDPYKLSSEL----VDNLGLLLHQVVVDSENAS 881
               KL E  E K  E  ++  E++L   +  KL+ EL     +N  L     +  +EN  
Sbjct: 1374 ENEKLAEELELKAAENEKLAEELELKAAENEKLAEELELKAAENEKLAEELELKAAENEK 1433

Query: 882  VLLKGERDHKKVAISTLNKLTDQFE--ALENH--SNDLVIANIDLGVLFEYLKHHVDDAG 941
            +      +  ++  +   KL ++ E  A EN   + +L +   +   L E L+    +  
Sbjct: 1434 L-----AEELELKAAENEKLAEELELKAAENEKLAEELELKAAENEKLAEELELKAAENE 1493

Query: 942  GKIYELE---NLNESLKQQGLYHKISNCELAERLRGYDLKLTELQ--SQLCDLHQSSNEM 1001
                ELE     NE L ++       N +LAE L   +LK  E +  ++  +L  + NE 
Sbjct: 1494 KLAEELELKAAENEKLAEELELKAAENEKLAEEL---ELKAAENEKLAEELELKAAENEK 1553

Query: 1002 VSSTCN----QLDKLREGEIARATILEKDLQSFLLELAESIAKLDESLGKSDTAALKFCT 1025
            ++        + +KL E    +A   EK  +   L++AE+  KL E L       LK   
Sbjct: 1554 LAEELELKAAENEKLAEELELKAAENEKLAEELELKVAEN-EKLAEEL------ELKVAE 1592

BLAST of MC11g0525 vs. ExPASy Swiss-Prot
Match: O76329 (Interaptin OS=Dictyostelium discoideum OX=44689 GN=abpD PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 3.8e-06
Identity = 177/950 (18.63%), Postives = 403/950 (42.42%), Query Frame = 0

Query: 1196 EKSGSLMMKLTELQESVHDLSTLILDHECEIVILKESLSQVQEALVASRSV-LKDKANEL 1255
            EK   L  ++ + Q     L+  + + + EI  L     Q Q+  + +  + +K+K   +
Sbjct: 657  EKQTQLQSEIKDNQTINEQLNKQLSEKDKEIEKLSNQQEQQQDEKINNLLLEIKEKDCLI 716

Query: 1256 EQSEQRVSAIREKLSIAVAKGKGLIVQRDNLKQSLAQTSSELERCLQELQMK-DTRLHE- 1315
            E+  Q+   + E + +  +K + L+++ +N K     +S E E  L ELQ K D R ++ 
Sbjct: 717  ERINQQ---LLENIDLN-SKYQQLLLEFENFK---LNSSKEKENQLNELQSKQDERFNQL 776

Query: 1316 ---------------------TETKLTAYSEAGERVEALESELSYIRNSATALRESFLLK 1375
                                  + +L++ S   +++++   ELS ++          + K
Sbjct: 777  NDEKLEKEKQLQSIEDEFNQYKQQQLSSNSNIDQQLQSTIIELSELKEQKELNDSKLIEK 836

Query: 1376 DSVLQRIEEILDELDLPENFHSRDIIEKVDWLAKSSTGENLPHTDWDQRSSVAGGSGSDA 1435
            +  LQ++++  D+L+       +D +E ++   K    E   +   ++ +       +  
Sbjct: 837  EKQLQQLQQEFDQLNEKNQKDHQDQLELLEKQLKQLQQE---YDQLNETNQSIENQLNQQ 896

Query: 1436 NFVIMDAWKDEVQMDANVGDDLRRKYEELQTRFYGLAEQNEMLEQSLMERN----NIVQR 1495
            N +  +   ++ Q    + + L ++ E++Q      ++QN +  + + E+N     + Q 
Sbjct: 897  NLINKENLNEKEQELLKLQNQLNQQIEKIQFDQQEFSKQNSINIELVNEKNEKLIQLQQD 956

Query: 1496 WEELLEK---IDIPSQLRSMEPEDKMEWLHKSLSEAFHDRDSLHQRVNYLENYCGLLTAD 1555
            +++L ++    D   +   +E E++++ +   L++     +S H+     +     +  D
Sbjct: 957  YDQLKQQNRSNDEKDENDLIEKENQLKSIQNELNQLIEKNESDHKEQQLKQQ---SIEND 1016

Query: 1556 LDDSQKKISDLEAELHSLVLER-EKLSEKLEIIYHHNENLAFGNFENEVGIIVLQNELSN 1615
            L + + +I  L+++L+    ++  +LSEK + +    E   F   E ++    ++N+L  
Sbjct: 1017 LIEKENQIQQLQSQLNEQRQQQSNQLSEKDQQLNQLIEKNQFDQKEQQLKQQSIENDLFE 1076

Query: 1616 MQENLISTEHKIVKLEALVSNAL--QDEDVNDLVSGSSIEFLDLMVTKLIQNYTASSSGK 1675
             +  +   + ++ +     SN L  +D+ +N L+  +  +  +       Q     S   
Sbjct: 1077 KENQIQQLQSQLNEQRQQQSNQLSEKDQQLNQLIEKNESDQKE-------QQLKQQSIEN 1136

Query: 1676 AVLGKATNGHDTEEEMLARSKNMHDAWQNDINI-------LKKELEDALNQLMVVTEER- 1735
             ++ K     + + + L    N     Q++++I       L+K+L+   + L+ + +E+ 
Sbjct: 1137 DLIEK-----ENQIQQLQLQLNEQRQLQSEVSIDNDKILELEKQLKQCQSDLLKLNDEKQ 1196

Query: 1736 -------DQYMEMHESLVVKVESSDKKKDELQELLNLEEQKSASIREKLNVAVRKGKSLI 1795
                   D+ +E  +  +   +  + K  +  +L + ++Q+  SI++ LN   ++ +   
Sbjct: 1197 QQDKQLQDKQIEFDQLQLTFNQFKNDKDSQFIQLQDDQKQQLQSIQQDLNQLKQENQEKE 1256

Query: 1796 QQRDSLKQAVEEMTTELKHLRSEMKSQENTLASYEQNF-----------KDFSVYSGRAE 1855
            +Q     + ++ +  E +    ++  ++  L S +QN            K FS    + +
Sbjct: 1257 KQLSEKDEKLQSIQFENQEKEKQLSEKDEKLQSIQQNLNQLNDENQEKVKQFSEKDEKLQ 1316

Query: 1856 ALESENLSLKNRLAEIERNFQEKEHKLSSIINTLVHIEDNVDVSENDPI-EKLKQVGKLC 1915
            +++ +   LK    E E+   EK+ KL SI   L  + D+  + +N+ + EK +Q+ KL 
Sbjct: 1317 SIQQDLNQLKQENQEKEKQLSEKDEKLQSIQQDLNQLNDD-QIKKNEKLKEKEEQLLKLQ 1376

Query: 1916 SDLREAM---------YFSEQESVKSRRAAELLLAELNEVQERNDTFQ--------EELA 1975
             D  +             SE+E+   +   E  + +LN+ Q+ N+  Q        ++  
Sbjct: 1377 QDFNDQQSQQLKQLEEKLSEKENQLQQLKQENEINQLNQQQQSNEIIQQLKDQLLKQQQQ 1436

Query: 1976 KASDEIAELTKERDLAETSKLEALSELEKLSTLHSKEKKIQYSQ---------------- 2035
            +  +   E   ER + E  +L+   E+++ S L +KE KIQ +Q                
Sbjct: 1437 EQQENNNEKEIERLIQEIEQLKQQQEIDQ-SELSNKEIKIQTTQQEFDQLSHNRSKDQLH 1496

Query: 2036 FMGLKSGLDRLKEAMREINCLLADAFSKDLDAFYNLEAAIQSCTEPNGPADVNLSHSIVS 2052
               L+  LD+LK++  + +      F K +D  YNL+  ++  T  N   D  L   +  
Sbjct: 1497 LQQLQQELDQLKQSFDDQD----HQFKKVIDERYNLQLQLEQSTLSNNQLDQLLKEKLKP 1556

BLAST of MC11g0525 vs. ExPASy Swiss-Prot
Match: Q02224 (Centromere-associated protein E OS=Homo sapiens OX=9606 GN=CENPE PE=1 SV=2)

HSP 1 Score: 54.7 bits (130), Expect = 1.9e-05
Identity = 273/1352 (20.19%), Postives = 544/1352 (40.24%), Query Frame = 0

Query: 1255 EQSEQRVSAIREKLSIAVAKGKGLIVQRDNLKQSLAQTSSELE-RCLQELQMKDTR---L 1314
            E  E  ++++R      V   + L  +++ ++  L + +   E   L+    KD     +
Sbjct: 494  ENIESELNSLRADYDNLVLDYEQLRTEKEEMELKLKEKNDLDEFEALERKTKKDQEMQLI 553

Query: 1315 HETETKLTAYSEAGERVEALESELSYIRNSATALRESFLLKDSVLQRIEEILDELDLPEN 1374
            HE          A    + LE+ELS   +    LRE    K+  +++++E +D   L EN
Sbjct: 554  HEISNLKNLVKHAEVYNQDLENELS---SKVELLRE----KEDQIKKLQEYIDSQKL-EN 613

Query: 1375 F-----HSRDIIEKVDWLAKSSTGENLPHTDWDQRSSVAGGSGSDANFVIMDAWKDEVQM 1434
                  +S + IE    + ++         D  + S+         N  + +  K+    
Sbjct: 614  IKMDLSYSLESIEDPKQMKQTLFDAETVALDAKRESAFL----RSENLELKEKMKELATT 673

Query: 1435 DANVGDDLRRKYEELQTRFYGLAEQNEMLEQSLMERNNIVQRWEELLEKIDIPSQLRSME 1494
               + +D++    +L+ +     +    LE+ L    N + +   L++       L ++E
Sbjct: 674  YKQMENDIQLYQSQLEAK----KKMQVDLEKELQSAFNEITKLTSLIDGKVPKDLLCNLE 733

Query: 1495 PEDKMEWLHKSLSEAFHDRDSLHQRVNYLENYCGLLTADLDDSQKKISDLEAELHSLVLE 1554
             E K+  L K L++   + ++L + V  L      L ++++  +K+I D   ELH +  E
Sbjct: 734  LEGKITDLQKELNKEVEENEALREEVILLSELKS-LPSEVERLRKEIQDKSEELHIITSE 793

Query: 1555 REKLSEKLEIIYHHNENLAFGNFENEVGIIVLQNELSNMQENLISTEHKIVKLEALVSNA 1614
            ++KL    E++  H E+   G  E E+G    +++L+  Q N  ST+ +    + L  + 
Sbjct: 794  KDKLFS--EVV--HKESRVQGLLE-EIG--KTKDDLATTQSNYKSTDQEFQNFKTLHMD- 853

Query: 1615 LQDEDVNDLVSGSSIEFLDLMVTKLIQNYTASSSGKAVLGKATNGHDTEEEMLARSKNMH 1674
                                      Q Y      K VL          +E++  SK   
Sbjct: 854  ------------------------FEQKY------KMVL---EENERMNQEIVNLSKEA- 913

Query: 1675 DAWQNDINILKKELEDALNQLMVVTEERDQYMEMHESLVVKVESSDKKKDELQELLNLEE 1734
              + + +  LK EL     +L   T E  + +   E L  ++E+ D         L   E
Sbjct: 914  QKFDSSLGALKTELSYKTQELQEKTREVQERLNEMEQLKEQLENRD-------STLQTVE 973

Query: 1735 QKSASIREKLNVAVRKGKSLIQQRDSLKQAVEEMTTELKHLRSEM-----------KSQE 1794
            ++   I EKL   + + K+L Q++D LKQ  E +  E   L+S++           +   
Sbjct: 974  REKTLITEKLQQTLEEVKTLTQEKDDLKQLQESLQIERDQLKSDIHDTVNMNIDTQEQLR 1033

Query: 1795 NTLASYEQNFKDFSVYSGRAEALESENLSLKNRLAEIERNFQEKEHKLSSIINTLVHIED 1854
            N L S +Q+ +  +    +     S NL ++    E +  FQ+K   +    +       
Sbjct: 1034 NALESLKQHQETINTLKSKISEEVSRNLHMEENTGETKDEFQQKMVGIDKKQDLEAKNTQ 1093

Query: 1855 NV--DVSENDPIEKLKQVGKLCSDLREAMYFSEQESVKSRRAAELLLAELNEVQERNDTF 1914
             +  DV +N+ IE+ +++  L  +  E       ESV + +  E L  +L E  E     
Sbjct: 1094 TLTADVKDNEIIEQQRKIFSLIQEKNELQQM--LESVIAEK--EQLKTDLKENIEMTIEN 1153

Query: 1915 QEELAKASDEIAE------------LTKERDLAETSKLEALSELEKLSTLHSKEKKIQYS 1974
            QEEL    DE+ +            + KE +L+ T   + L+E+E+     S++ + +  
Sbjct: 1154 QEELRLLGDELKKQQEIVAQEKNHAIKKEGELSRT--CDRLAEVEEKLKEKSQQLQEKQQ 1213

Query: 1975 QFMGLKSGLDRLKEAMREINCLLADAFSKDLDAFYNLEAAIQSCTEPNGPADVNLSHSIV 2034
            Q + ++  +  +++ + EI  L  +  +K+L   +     ++   +      +N ++  V
Sbjct: 1214 QLLNVQEEMSEMQKKINEIENLKNELKNKELTLEHMETERLELAQK------LNENYEEV 1273

Query: 2035 SGAFKSRD-LKDKGKFFSLDSWSNFYTNAHVDENVATEIHS-----LVH-QLEESMKEIG 2094
                K R  LK+  K F  +     +   ++ E  AT + +     + H  L+E  + I 
Sbjct: 1274 KSITKERKVLKELQKSFETE---RDHLRGYIREIEATGLQTKEELKIAHIHLKEHQETID 1333

Query: 2095 ALKEMIDG------HSVSFHKQSDSLSKILGVLY---------REVNSQKELVQALKWDV 2154
             L+  +        ++    K    L + + VL+         +EV+  +E +  L+   
Sbjct: 1334 ELRRSVSEKTAQIINTQDLEKSHTKLQEEIPVLHEEQELLPNVKEVSETQETMNELELLT 1393

Query: 2155 QQS---ESVAKDR-EMEGDILCRNIAMLFEACTSVIKEVDERKGELMGHDLTSGNLGMEI 2214
            +QS   +S    R EME   L        E   S+ KE D  K      ++    L   I
Sbjct: 1394 EQSTTKDSTTLARIEMERLRLNEKFQESQEEIKSLTKERDNLKTIKEALEVKHDQLKEHI 1453

Query: 2215 ISTTPDQLSHAGKTHLLSEES--VRKIADRLLLAVREFIGFKAEMLDGSLKEMKVSVANL 2274
              T    L+   ++    E+S  +++  +     V E   FK +  D +L  +++ +  L
Sbjct: 1454 RET----LAKIQESQSKQEQSLNMKEKDNETTKIVSEMEQFKPK--DSALLRIEIEMLGL 1513

Query: 2275 QKELQEKDIQKERICMELVGQIKEAEATASRYSIDLQASKDQVYELEKVTEQMDSERKVL 2334
             K LQE   + + +  E                      KD   +L+++ E + SE   L
Sbjct: 1514 SKRLQESHDEMKSVAKE----------------------KD---DLQRLQEVLQSESDQL 1573

Query: 2335 EQRLREMQDGLSISDELRERVRLLTGSLAAKDQEIEALMHALDEEELQMEGLTKKIEDLE 2394
            ++ ++E+   ++   E  E +++    L  +++ I  L   L E+E ++  + K++E + 
Sbjct: 1574 KENIKEI---VAKHLETEEELKVAHCCLKEQEETINELRVNLSEKETEISTIQKQLEAIN 1633

Query: 2395 RDLKQKNQELESIEASRGKLMKKLSITVTKFDELHHLSE----------SLLTEVEKLQA 2454
              L+ K QE+   E      +K++S    K +EL    E          S+ +++ +L  
Sbjct: 1634 DKLQNKIQEIYEKEEQFN--IKQISEVQEKVNELKQFKEHRKAKDSALQSIESKMLELTN 1693

Query: 2455 QLQDRDAEISFL---RQEVTRCTNDALVATQTSNRNSEDINEVITWFDTMEARVGLSHSG 2514
            +LQ+   EI  +   ++E+ R      +       N+++I   +      E +  L  + 
Sbjct: 1694 RLQESQEEIQIMIKEKEEMKRVQEALQIERDQLKENTKEIVAKMKESQEKEYQF-LKMTA 1726

Query: 2515 HDDKENEVRECKEVLKKKITSILKGIEDLQAESQRKDEML---LAEKNKV----EELKRK 2525
             ++ + ++ E  E LK++  +    +E+++ E+ R  ++L   L E   V    ++L+  
Sbjct: 1754 VNETQEKMCEI-EHLKEQFETQKLNLENIETENIRLTQILHENLEEMRSVTKERDDLRSV 1726

BLAST of MC11g0525 vs. NCBI nr
Match: XP_022133353.1 (centromere-associated protein E [Momordica charantia])

HSP 1 Score: 4865 bits (12620), Expect = 0.0
Identity = 2630/2698 (97.48%), Postives = 2630/2698 (97.48%), Query Frame = 0

Query: 2    LQQFRKKKDNKGTGSQGNSSKNKSKLEQQDADTEIVNVAAKPTSGSYSTDGVLASSFDCD 61
            LQQFRKKKDNKGTGSQGNSSKNKSKLEQQDADTEIVNVAAKPTSGSYSTDGVLASSFDCD
Sbjct: 18   LQQFRKKKDNKGTGSQGNSSKNKSKLEQQDADTEIVNVAAKPTSGSYSTDGVLASSFDCD 77

Query: 62   ADNVDSSASPSEHSSAAEIDHSTVYLKQEMDLAETSAIDEAEIPVEEVGYREDCDRLIQN 121
            ADNVDSSASPSEHSSAAEIDHSTVYLKQEMDLAETSAIDEAEIPVEEVGYREDCDRLIQN
Sbjct: 78   ADNVDSSASPSEHSSAAEIDHSTVYLKQEMDLAETSAIDEAEIPVEEVGYREDCDRLIQN 137

Query: 122  AEAAGVMSSGPSILIDAEVNNNHLCNLSSTESSSQISSASVDEQGRMVEVWSGCREEEIS 181
            AEAAGVMSSGPSILIDAEVNNNHLCNLSSTESSSQISSASVDEQGRMVEVWSGCREEEIS
Sbjct: 138  AEAAGVMSSGPSILIDAEVNNNHLCNLSSTESSSQISSASVDEQGRMVEVWSGCREEEIS 197

Query: 182  PARSASLLQAREDVGMTEDASMQSDQVCETQLAEDKQLQTGGMYESAAETTFKDTHCDEE 241
            PARSASLLQAREDVGMTEDASMQSDQVCETQLAEDKQLQTGGMYESAAETTFKDTHCDEE
Sbjct: 198  PARSASLLQAREDVGMTEDASMQSDQVCETQLAEDKQLQTGGMYESAAETTFKDTHCDEE 257

Query: 242  EIITVDVASVSGFATVSNDFSISNPGENLGMQSSSSSSRDDWKEERQVHAEDMMHPSMYQ 301
            EIITVDVASVSGFATVSNDFSISNPGENLGMQSSSSSSRDDWKEERQVHAEDMMHPSMYQ
Sbjct: 258  EIITVDVASVSGFATVSNDFSISNPGENLGMQSSSSSSRDDWKEERQVHAEDMMHPSMYQ 317

Query: 302  VQYMPEDNFAVQSEGHERPSQTSAKISEGGDADAIFPNAHMTTDLAVQSGTFYSFGQESE 361
            VQYMPEDNFAVQSEGHERPSQTSAKISEGGDADAIFPNAHMTTDLAVQSGTFYSFGQESE
Sbjct: 318  VQYMPEDNFAVQSEGHERPSQTSAKISEGGDADAIFPNAHMTTDLAVQSGTFYSFGQESE 377

Query: 362  FLDLLERVKEELIVTSFSKDISNLQISEQNVLQMELDNQHRKLTNDMSLVNTSLNEILER 421
            FLDLLERVKEELIVTSFSKDISNLQISEQNVLQMELDNQHRKLTNDMSLVNTSLNEILER
Sbjct: 378  FLDLLERVKEELIVTSFSKDISNLQISEQNVLQMELDNQHRKLTNDMSLVNTSLNEILER 437

Query: 422  NQSLVDELSQCRSELRDVSSAREELKNQLLTAEAEIENLSSRATESEKNLEKFHADMFRL 481
            NQSLVDELSQCRSELRDVSSAREELKNQLLTAEAEIENLSSRATESEKNLEKFHADMFRL
Sbjct: 438  NQSLVDELSQCRSELRDVSSAREELKNQLLTAEAEIENLSSRATESEKNLEKFHADMFRL 497

Query: 482  SKELDDCKHLVTALEEENERLNGVITSENENKMKLAEEKELYINENEKISSELSSFKSLK 541
            SKELDDCKHLVTALEEENERLNGVITSENENKMKLAEEKELYINENEKISSELSSFKSLK
Sbjct: 498  SKELDDCKHLVTALEEENERLNGVITSENENKMKLAEEKELYINENEKISSELSSFKSLK 557

Query: 542  VALEDENSKLIGSLSSVAEGKTKLEEEREQLFQVNGTLSVELENCRNLIATQQEEITNLI 601
            VALEDENSKLIGSLSSVAEGKTKLEEEREQLFQVNGTLSVELENCRNLIATQQEEITNLI
Sbjct: 558  VALEDENSKLIGSLSSVAEGKTKLEEEREQLFQVNGTLSVELENCRNLIATQQEEITNLI 617

Query: 602  KNLALVTEDRTKLEEDKTLLFHENEKMASELLVLDERLSAEYGERVRFEDDLRDALVRLE 661
            KNLALVTEDRTKLEEDKTLLFHENEKMASELLVLDERLSAEYGERVRFEDDLRDALVRLE
Sbjct: 618  KNLALVTEDRTKLEEDKTLLFHENEKMASELLVLDERLSAEYGERVRFEDDLRDALVRLE 677

Query: 662  KLTEENIFLSNSLDIQKSKTEELCGEILPKQMRSIEDGNQTETADSGWHRVNKSQENDAY 721
            KLTEENIFLSNSLDIQKSKTEELCGEILPKQMRSIEDGNQTETADSGWHRVNKSQENDAY
Sbjct: 678  KLTEENIFLSNSLDIQKSKTEELCGEILPKQMRSIEDGNQTETADSGWHRVNKSQENDAY 737

Query: 722  QIKKQELFDDSFEFITLGRHLEEAYLVLQKLEKEIKGLQSNSASFSRSGSKVAAPAVSKL 781
            QIKKQELFDDSFEFITLGRHLEEAYLVLQKLEKEIKGLQSNSASFSRSGSKVAAPAVSKL
Sbjct: 738  QIKKQELFDDSFEFITLGRHLEEAYLVLQKLEKEIKGLQSNSASFSRSGSKVAAPAVSKL 797

Query: 782  IEAFETKVNEEHEVESEIQLPEDPYKLSSELVDNLGLLLHQVVVDSENASVLLKGERDHK 841
            IEAFETKVNEEHEVESEIQLPEDPYKLSSELVDNLGLLLHQVVVDSENASVLLKGERDHK
Sbjct: 798  IEAFETKVNEEHEVESEIQLPEDPYKLSSELVDNLGLLLHQVVVDSENASVLLKGERDHK 857

Query: 842  KVAISTLNKLTDQFEALENHSNDLVIANIDLGVLFEYLKHHVDDAGGKIYELENLNESLK 901
            KVAISTLNKLTDQFEALENHSNDLVIANIDLGVLFEYLKHHVDDAGGKIYELENLNESLK
Sbjct: 858  KVAISTLNKLTDQFEALENHSNDLVIANIDLGVLFEYLKHHVDDAGGKIYELENLNESLK 917

Query: 902  QQGLYHKISNCELAERLRGYDLKLTELQSQLCDLHQSSNEMVSSTCNQLDKLREGEIARA 961
            QQGLYHKISNCELAERLRGYDLKLTELQSQLCDLHQSSNEMVSSTCNQLDKLREGEIARA
Sbjct: 918  QQGLYHKISNCELAERLRGYDLKLTELQSQLCDLHQSSNEMVSSTCNQLDKLREGEIARA 977

Query: 962  TILEKDLQSFLLELAESIAKLDESLGKSDTAALKFCTNDQLPSCIIASVIDAVKMIDDLR 1021
            TILEKDLQSFLLELAESIAKLDESLGKSDTAALKFCTNDQLPSCIIASVIDAVKMIDDLR
Sbjct: 978  TILEKDLQSFLLELAESIAKLDESLGKSDTAALKFCTNDQLPSCIIASVIDAVKMIDDLR 1037

Query: 1022 ERLQATGADREAFRMLYEEVNEKYDNLFRRTEFSVDMLHRIYSELQKLYIASCGSVGGSD 1081
            ERLQATGADREAFRMLYEEVNEKYDNLFRRTEFSVDMLHRIYSELQKLYIASCGSVGGSD
Sbjct: 1038 ERLQATGADREAFRMLYEEVNEKYDNLFRRTEFSVDMLHRIYSELQKLYIASCGSVGGSD 1097

Query: 1082 MNMQIKGLGDPLDYSSFEAFIKLLEDCITERLQLESVNNKLRLDLEHMNVGFVDFSKRCL 1141
            MNMQIKGLGDPLDYSSFEAFIKLLEDCITERLQLESVNNKLRLDLEHMNVGFVDFSKRCL
Sbjct: 1098 MNMQIKGLGDPLDYSSFEAFIKLLEDCITERLQLESVNNKLRLDLEHMNVGFVDFSKRCL 1157

Query: 1142 DSPGIKKLITDVQSVLLLDDAEMDVEMPALYLESMVSLLLQKYKETELQLGLSREKSGSL 1201
            DSPGIKKLITDVQSVLLLDDAEMDVEMPALYLESMVSLLLQKYKETELQLGLSREKSGSL
Sbjct: 1158 DSPGIKKLITDVQSVLLLDDAEMDVEMPALYLESMVSLLLQKYKETELQLGLSREKSGSL 1217

Query: 1202 MMKLTELQESVHDLSTLILDHECEIVILKESLSQVQEALVASRSVLKDKANELEQSEQRV 1261
            MMKLTELQESVHDLSTLILDHECEIVILKESLSQVQEALVASRSVLKDKANELEQSEQRV
Sbjct: 1218 MMKLTELQESVHDLSTLILDHECEIVILKESLSQVQEALVASRSVLKDKANELEQSEQRV 1277

Query: 1262 SAIREKLSIAVAKGKGLIVQRDNLKQSLAQTSSELERCLQELQMKDTRLHETETKLTAYS 1321
            SAIREKLSIAVAKGKGLIVQRDNLKQSLAQTSSELERCLQELQMKDTRLHETETKLTAYS
Sbjct: 1278 SAIREKLSIAVAKGKGLIVQRDNLKQSLAQTSSELERCLQELQMKDTRLHETETKLTAYS 1337

Query: 1322 EAGERVEALESELSYIRNSATALRESFLLKDSVLQRIEEILDELDLPENFHSRDIIEKVD 1381
            EAGERVEALESELSYIRNSATALRESFLLKDSVLQRIEEILDELDLPENFHSRDIIEKVD
Sbjct: 1338 EAGERVEALESELSYIRNSATALRESFLLKDSVLQRIEEILDELDLPENFHSRDIIEKVD 1397

Query: 1382 WLAKSSTGENLPHTDWDQRSSVAGGSGSDANFVIMDAWKDEVQMDANVGDDLRRKYEELQ 1441
            WLAKSSTGENLPHTDWDQRSSVAGGSGSDANFVIMDAWKDEVQMDANVGDDLRRKYEELQ
Sbjct: 1398 WLAKSSTGENLPHTDWDQRSSVAGGSGSDANFVIMDAWKDEVQMDANVGDDLRRKYEELQ 1457

Query: 1442 TRFYGLAEQNEMLEQSLMERNNIVQRWEELLEKIDIPSQLRSMEPEDKMEWLHKSLSEAF 1501
            TRFYGLAEQNEMLEQSLMERNNIVQRWEELLEKIDIPSQLRSMEPEDKMEWLHKSLSEAF
Sbjct: 1458 TRFYGLAEQNEMLEQSLMERNNIVQRWEELLEKIDIPSQLRSMEPEDKMEWLHKSLSEAF 1517

Query: 1502 HDRDSLHQRVNYLENYCGLLTADLDDSQKKISDLEAELHSLVLEREKLSEKLEIIYHHNE 1561
            HDRDSLHQRVNYLENYCGLLTADLDDSQKKISDLEAELHSLVLEREKLSEKLEIIYHHNE
Sbjct: 1518 HDRDSLHQRVNYLENYCGLLTADLDDSQKKISDLEAELHSLVLEREKLSEKLEIIYHHNE 1577

Query: 1562 NLAFGNFENEVGIIVLQNELSNMQENLISTEHKIVKLEALVSNALQDEDVNDLVSGSSIE 1621
            NLAFGNFENEVGIIVLQNELSNMQENLISTEHKIVKLEALVSNALQDEDVNDLVSGSSIE
Sbjct: 1578 NLAFGNFENEVGIIVLQNELSNMQENLISTEHKIVKLEALVSNALQDEDVNDLVSGSSIE 1637

Query: 1622 FLDLMVTKLIQNYTASSSGKAVLGKATNGHDTEEEMLARSKNMHDAWQNDINILKKELED 1681
            FLDLMVTKLIQNYTASSSGKAVLGKATNGHDTEEEMLARSKNMHDAWQNDINILKKELED
Sbjct: 1638 FLDLMVTKLIQNYTASSSGKAVLGKATNGHDTEEEMLARSKNMHDAWQNDINILKKELED 1697

Query: 1682 ALNQLMVVTEERDQYMEMHESLVVKV---------------------------------- 1741
            ALNQLMVVTEERDQYMEMHESLVVKV                                  
Sbjct: 1698 ALNQLMVVTEERDQYMEMHESLVVKVGSSDKKKDELQELLNLEEQKSASMREKLEDAWHQ 1757

Query: 1742 ----------------------ESSDKKKDELQELLNLEEQKSASIREKLNVAVRKGKSL 1801
                                  ESSDKKKDELQELLNLEEQKSASIREKLNVAVRKGKSL
Sbjct: 1758 LMVVTEERDQYMEMHESLVVKVESSDKKKDELQELLNLEEQKSASIREKLNVAVRKGKSL 1817

Query: 1802 IQQRDSLKQAVEEMTTELKHLRSEMKSQENTLASYEQNFKDFSVYSGRAEALESENLSLK 1861
            IQQRDSLKQAVEEMTTELKHLRSEMKSQENTLASYEQNFKDFSVYSGRAEALESENLSLK
Sbjct: 1818 IQQRDSLKQAVEEMTTELKHLRSEMKSQENTLASYEQNFKDFSVYSGRAEALESENLSLK 1877

Query: 1862 NRLAEIERNFQEKEHKLSSIINTLVHIEDNVDVSENDPIEKLKQVGKLCSDLREAMYFSE 1921
            NRLAEIERNFQEKEHKLSSIINTLVHIEDNVDVSENDPIEKLKQVGKLCSDLREAMYFSE
Sbjct: 1878 NRLAEIERNFQEKEHKLSSIINTLVHIEDNVDVSENDPIEKLKQVGKLCSDLREAMYFSE 1937

Query: 1922 QESVKSRRAAELLLAELNEVQERNDTFQEELAKASDEIAELTKERDLAETSKLEALSELE 1981
            QESVKSRRAAELLLAELNEVQERNDTFQEELAKASDEIAELTKERDLAETSKLEALSELE
Sbjct: 1938 QESVKSRRAAELLLAELNEVQERNDTFQEELAKASDEIAELTKERDLAETSKLEALSELE 1997

Query: 1982 KLSTLHSKEKKIQYSQFMGLKSGLDRLKEAMREINCLLADAFSKDLDAFYNLEAAIQSCT 2041
            KLSTLHSKEKKIQYSQFMGLKSGLDRLKEAMREINCLLADAFSKDLDAFYNLEAAIQSCT
Sbjct: 1998 KLSTLHSKEKKIQYSQFMGLKSGLDRLKEAMREINCLLADAFSKDLDAFYNLEAAIQSCT 2057

Query: 2042 EPNGPADVNLSHSIVSGAFKSRDLKDKGKFFSLDSWSNFYTNAHVDENVATEIHSLVHQL 2101
            EPNGPADVNLSHSIVSGAFKSRDLKDKGKFFSLDSWSNFYTNAHVDENVATEIHSLVHQL
Sbjct: 2058 EPNGPADVNLSHSIVSGAFKSRDLKDKGKFFSLDSWSNFYTNAHVDENVATEIHSLVHQL 2117

Query: 2102 EESMKEIGALKEMIDGHSVSFHKQSDSLSKILGVLYREVNSQKELVQALKWDVQQSESVA 2161
            EESMKEIGALKEMIDGHSVSFHKQSDSLSKILGVLYREVNSQKELVQALKWDVQQSESVA
Sbjct: 2118 EESMKEIGALKEMIDGHSVSFHKQSDSLSKILGVLYREVNSQKELVQALKWDVQQSESVA 2177

Query: 2162 KDREMEGDILCRNIAMLFEACTSVIKEVDERKGELMGHDLTSGNLGMEIISTTPDQLSHA 2221
            KDREMEGDILCRNIAMLFEACTSVIKEVDERKGELMGHDLTSGNLGMEIISTTPDQLSHA
Sbjct: 2178 KDREMEGDILCRNIAMLFEACTSVIKEVDERKGELMGHDLTSGNLGMEIISTTPDQLSHA 2237

Query: 2222 GKTHLLSEESVRKIADRLLLAVREFIGFKAEMLDGSLKEMKVSVANLQKELQEKDIQKER 2281
            GKTHLLSEESVRKIADRLLLAVREFIGFKAEMLDGSLKEMKVSVANLQKELQEKDIQKER
Sbjct: 2238 GKTHLLSEESVRKIADRLLLAVREFIGFKAEMLDGSLKEMKVSVANLQKELQEKDIQKER 2297

Query: 2282 ICMELVGQIKEAEATASRYSIDLQASKDQVYELEKVTEQMDSERKVLEQRLREMQDGLSI 2341
            ICMELVGQIKEAEATASRYSIDLQASKDQVYELEKVTEQMDSERKVLEQRLREMQDGLSI
Sbjct: 2298 ICMELVGQIKEAEATASRYSIDLQASKDQVYELEKVTEQMDSERKVLEQRLREMQDGLSI 2357

Query: 2342 SDELRERVRLLTGSLAAKDQEIEALMHALDEEELQMEGLTKKIEDLERDLKQKNQELESI 2401
            SDELRERVRLLTGSLAAKDQEIEALMHALDEEELQMEGLTKKIEDLERDLKQKNQELESI
Sbjct: 2358 SDELRERVRLLTGSLAAKDQEIEALMHALDEEELQMEGLTKKIEDLERDLKQKNQELESI 2417

Query: 2402 EASRGKLMKKLSITVTKFDELHHLSESLLTEVEKLQAQLQDRDAEISFLRQEVTRCTNDA 2461
            EASRGKLMKKLSITVTKFDELHHLSESLLTEVEKLQAQLQDRDAEISFLRQEVTRCTNDA
Sbjct: 2418 EASRGKLMKKLSITVTKFDELHHLSESLLTEVEKLQAQLQDRDAEISFLRQEVTRCTNDA 2477

Query: 2462 LVATQTSNRNSEDINEVITWFDTMEARVGLSHSGHDDKENEVRECKEVLKKKITSILKGI 2521
            LVATQTSNRNSEDINEVITWFDTMEARVGLSHSGHDDKENEVRECKEVLKKKITSILKGI
Sbjct: 2478 LVATQTSNRNSEDINEVITWFDTMEARVGLSHSGHDDKENEVRECKEVLKKKITSILKGI 2537

Query: 2522 EDLQAESQRKDEMLLAEKNKVEELKRKELQLNLLEDVGDNNRASSVAPEIFESEPLHIVK 2581
            EDLQAESQRKDEMLLAEKNKVEELKRKELQLNLLEDVGDNNRASSVAPEIFESEPL    
Sbjct: 2538 EDLQAESQRKDEMLLAEKNKVEELKRKELQLNLLEDVGDNNRASSVAPEIFESEPL---- 2597

Query: 2582 SCILCTLQINKWAASSTSVTPQVRSLRKGNTTDQVAIAIDMDPASSSNRLEDEDDDKVHG 2641
                    INKWAASSTSVTPQVRSLRKGNTTDQVAIAIDMDPASSSNRLEDEDDDKVHG
Sbjct: 2598 --------INKWAASSTSVTPQVRSLRKGNTTDQVAIAIDMDPASSSNRLEDEDDDKVHG 2657

Query: 2642 FKSLASSRIVPKFSRRMTDMIDGLWVSCDRALMRQPALRLGIIIYWAILHALVATFVV 2643
            FKSLASSRIVPKFSRRMTDMIDGLWVSCDRALMRQPALRLGIIIYWAILHALVATFVV
Sbjct: 2658 FKSLASSRIVPKFSRRMTDMIDGLWVSCDRALMRQPALRLGIIIYWAILHALVATFVV 2703

BLAST of MC11g0525 vs. NCBI nr
Match: XP_038897845.1 (centromere-associated protein E isoform X1 [Benincasa hispida])

HSP 1 Score: 3859 bits (10007), Expect = 0.0
Identity = 2133/2670 (79.89%), Postives = 2336/2670 (87.49%), Query Frame = 0

Query: 2    LQQFRKKKDNKGTGSQGNSSKNKSKLEQQDADTE--IVNVAAKPTSGSYSTDGVLASSFD 61
            LQQFRKKKDNKG+GSQG+SSKNKSKLEQ DAD +  I   AAK TSG YSTDGVL SS D
Sbjct: 18   LQQFRKKKDNKGSGSQGSSSKNKSKLEQHDADADADIATGAAKSTSGRYSTDGVLISSVD 77

Query: 62   CDADNVDSSASPS-EHSSAAEIDHSTVYLKQEMDLAETSAIDEAEIPVEEVGYREDCDRL 121
            CD D VDSSASPS EHS AAEI+HSTV +KQEMDL ETSAID+AEIP++EVGYREDCD+ 
Sbjct: 78   CDPDTVDSSASPSTEHSLAAEINHSTVSVKQEMDLGETSAIDQAEIPMQEVGYREDCDQS 137

Query: 122  IQNAEAAGVMSSGPSILIDAEVNNNHLCNLSSTESSSQISSASVDEQGRMVEVWSGCREE 181
            IQNAEAAG +S  PS+  DAE NNNH+ NLSSTESSSQISSASV++QG++VEVW GCREE
Sbjct: 138  IQNAEAAGFVSFEPSLPTDAEENNNHISNLSSTESSSQISSASVEQQGKIVEVWGGCREE 197

Query: 182  EISPARSASLLQAREDVGMTEDASMQSDQVCETQLAEDKQLQTGGMYESAAETTFKDTHC 241
            E+ P++S SLLQAREDVGM EDA MQS QV ET+LA DKQL+TGG  ESAAETTFK+T C
Sbjct: 198  ELLPSQSTSLLQAREDVGM-EDALMQSGQVHETELAGDKQLETGGTSESAAETTFKETCC 257

Query: 242  DEEEIITVDVASVSGFATVSNDFSISNPGENLGMQSSSSSSRDDWKEERQVHAEDMMHPS 301
            +EEE+I  DV SVSG  T SN +SIS+PGE LGM++SSSS+RD+WKEERQVHAE  +H S
Sbjct: 258  NEEEVIATDVVSVSGAVTESNSYSISSPGEKLGMENSSSSNRDNWKEERQVHAEATIHSS 317

Query: 302  MYQVQYMPEDNFAVQSEGHERPSQTSAKISEGGDADAIFPNAHMTTDLAVQSGTFYSFGQ 361
              QVQY+PEDNFA QSEGHER SQTS KIS+ GD ++I  NAHMT     QSGTF SFGQ
Sbjct: 318  TCQVQYIPEDNFADQSEGHERASQTSVKISDAGDTNSISHNAHMTATSDAQSGTFSSFGQ 377

Query: 362  ESEFLDLLERVKEELIVTSFSKDISNLQISEQNVLQMELDNQHRKLTNDMSLVNTSLNEI 421
            + +F DLLER+KEELIVTSFSKDI N+QI+EQN LQMELDN   K TNDMSL+NTSLNE+
Sbjct: 378  DCKFFDLLERMKEELIVTSFSKDIFNMQITEQNELQMELDNHRYKSTNDMSLLNTSLNEV 437

Query: 422  LERNQSLVDELSQCRSELRDVSSAREELKNQLLTAEAEIENLSSRATESEKNLEKFHADM 481
            +ERNQSLVDELS CRSEL DVS A+EEL++QLLTAEAEIE LSSR +E+E +LEK H DM
Sbjct: 438  VERNQSLVDELSHCRSELVDVSIAKEELRDQLLTAEAEIEKLSSRTSETENSLEKLHGDM 497

Query: 482  FRLSKELDDCKHLVTALEEENERLNGVITSENENKMKLAEEKELYINENEKISSELSSFK 541
            FRL+KELD+CKHLVT LE ENERLNG+IT ENENK KLAEEKELY NENEKI SELSS K
Sbjct: 498  FRLTKELDECKHLVTMLEGENERLNGIITFENENKRKLAEEKELYDNENEKILSELSSLK 557

Query: 542  SLKVALEDENSKLIGSLSSVAEGKTKLEEEREQLFQVNGTLSVELENCRNLIATQQEEIT 601
            SLKVALE ENSKL+GSLSSVAE K+KLEEEREQLFQVNGTLSVEL NC++L+ATQQEEI 
Sbjct: 558  SLKVALEAENSKLMGSLSSVAEEKSKLEEEREQLFQVNGTLSVELANCKSLVATQQEEIM 617

Query: 602  NLIKNLALVTEDRTKLEEDKTLLFHENEKMASELLVLDERLSAEYGERVRFEDDLRDALV 661
            NL KNL LVTEDRTK++EDK  LFHENEKMASEL+VLDERLS E+ ERV+FE DL+DALV
Sbjct: 618  NLTKNLLLVTEDRTKVDEDKNRLFHENEKMASELIVLDERLSTEHEERVKFEGDLKDALV 677

Query: 662  RLEKLTEENIFLSNSLDIQKSKTEELCGEILPKQMRSIEDGNQTETADSGWHRVNKSQEN 721
            +L++LTEEN+FLSNSLDI K K EELCGEI+  Q RS ED +Q E ADS  +  NKSQ N
Sbjct: 678  QLDQLTEENVFLSNSLDIHKFKIEELCGEIMSLQTRSTEDEDQDENADSDQYHGNKSQGN 737

Query: 722  DAYQIK---------------------KQELFDDSFEFITLGRHLEEAYLVLQKLEKEIK 781
             + QI                      +QE FDDS  F+TLG+HL+EA L+LQKLEKEI 
Sbjct: 738  VSSQITFEKCLPDTSSVLAGGKPFMVTEQEFFDDSLGFVTLGKHLKEADLMLQKLEKEIT 797

Query: 782  GLQSNSASFSRSGSKVAAPAVSKLIEAFETKVN-EEHEVESEIQLPEDPYKLSSELVDNL 841
            GLQSNS S SRSGSK+AAPAVSKLI+AFE+KVN EEHEVE+EIQLP D + LS E+VDNL
Sbjct: 798  GLQSNSTS-SRSGSKMAAPAVSKLIQAFESKVNVEEHEVEAEIQLPNDSFNLSIEIVDNL 857

Query: 842  GLLLHQVVVDSENASVLLKGERDHKKVAISTLNKLTDQFEALENHSNDLVIANIDLGVLF 901
             +LL QVVVDSEN  +LLKGERD +KVAIS L++  D+FEALENHSNDLVIANI+ GVLF
Sbjct: 858  RVLLRQVVVDSENVIMLLKGERDDRKVAISKLSEFKDKFEALENHSNDLVIANIEHGVLF 917

Query: 902  EYLKHHVDDAGGKIYELENLNESLKQQGLYHKISNCELAERLRGYDLKLTELQSQLCDLH 961
            E LKHHVDDAG KIYELE LNESLKQ G++HK SN ELAERL GY+LKL EL+ QLCDLH
Sbjct: 918  ECLKHHVDDAGDKIYELEILNESLKQHGMHHKNSNSELAERLCGYELKLAELECQLCDLH 977

Query: 962  QSSNEMVSSTCNQLDKLREGEIARATILEKDLQSFLLELAESIAKLDESLGKSDTAALKF 1021
            QSSNEMVS  CNQLD L+EG I R   LEKD  SFLLELAE+ AKLDESLGKSDT+A+KF
Sbjct: 978  QSSNEMVSLICNQLDNLQEGAIERTMTLEKDWHSFLLELAETFAKLDESLGKSDTSAIKF 1037

Query: 1022 CTNDQLPSCIIASVIDAVKMIDDLRERLQATGADREAFRMLYEEVNEKYDNLFRRTEFSV 1081
            CT+DQLPSCI ASVIDAVK+IDDLRE+LQAT ++ E FR+L EEVNEKYDNLFR+TEFSV
Sbjct: 1038 CTSDQLPSCIAASVIDAVKVIDDLREKLQATASNGETFRILCEEVNEKYDNLFRKTEFSV 1097

Query: 1082 DMLHRIYSELQKLYIASCGSVGGSDMNMQIKGLGDPLDYSSFEAFIKLLEDCITERLQLE 1141
            +MLH+IY ELQKLYIASCGSV GSDMNMQ K LGDPLDYS+FEA IK LEDCI ERLQLE
Sbjct: 1098 NMLHKIYGELQKLYIASCGSVSGSDMNMQFKMLGDPLDYSNFEALIKPLEDCINERLQLE 1157

Query: 1142 SVNNKLRLDLEHMNVGFVDFSKRCLDSPGIKKLITDVQSVLLLDDAEMD-VEMPALYLES 1201
            SVN+KLR DL+H  V F +F +RCLDS GI+KLI DVQSVLLL+D E D  E+PA  LE 
Sbjct: 1158 SVNDKLRFDLQHRTVEFAEFRERCLDSIGIEKLIKDVQSVLLLEDTEKDRAEIPASRLEF 1217

Query: 1202 MVSLLLQKYKETELQLGLSREKSGSLMMKLTELQESVHDLSTLILDHECEIVILKESLSQ 1261
            MVSLLLQKY+E+ELQLGLSRE+S S+MMK TELQESVHDLS LILDHECEIV+LKESLSQ
Sbjct: 1218 MVSLLLQKYRESELQLGLSREESKSVMMKFTELQESVHDLSNLILDHECEIVLLKESLSQ 1277

Query: 1262 VQEALVASRSVLKDKANELEQSEQRVSAIREKLSIAVAKGKGLIVQRDNLKQSLAQTSSE 1321
             QEALVASRS LKDK NELEQ+EQRVSAIREKLSIAVAKGK LIVQRDNLKQSLAQTSSE
Sbjct: 1278 AQEALVASRSELKDKVNELEQAEQRVSAIREKLSIAVAKGKSLIVQRDNLKQSLAQTSSE 1337

Query: 1322 LERCLQELQMKDTRLHETETKLTAYSEAGERVEALESELSYIRNSATALRESFLLKDSVL 1381
            LERCLQELQMKDTRLHETE KL  YSEAGERVEALESELSYIRNSATALRESFLLKDSVL
Sbjct: 1338 LERCLQELQMKDTRLHETEMKLKIYSEAGERVEALESELSYIRNSATALRESFLLKDSVL 1397

Query: 1382 QRIEEILDELDLPENFHSRDIIEKVDWLAKSSTGENLPHTDWDQRSSVAGGSGSDANFVI 1441
            QRIEEILDELDLPENFHSRDIIEK+DWLAKSSTGENL HTDWDQRSSVAGGSGSDANF I
Sbjct: 1398 QRIEEILDELDLPENFHSRDIIEKIDWLAKSSTGENLVHTDWDQRSSVAGGSGSDANFAI 1457

Query: 1442 MDAWKDEVQMDANVGDDLRRKYEELQTRFYGLAEQNEMLEQSLMERNNIVQRWEELLEKI 1501
             DAWKDEVQ DANVGDDLRRKYEELQT+FYGLAEQNEMLEQSLMERN IVQRWEELLEKI
Sbjct: 1458 TDAWKDEVQPDANVGDDLRRKYEELQTKFYGLAEQNEMLEQSLMERNIIVQRWEELLEKI 1517

Query: 1502 DIPSQLRSMEPEDKMEWLHKSLSEAFHDRDSLHQRVNYLENYCGLLTADLDDSQKKISDL 1561
            DIPS LRSMEPEDK+EWLH+SLSEA HDRDSLHQRVNYLENYCG LTADLDDSQKKIS +
Sbjct: 1518 DIPSHLRSMEPEDKIEWLHRSLSEACHDRDSLHQRVNYLENYCGSLTADLDDSQKKISHI 1577

Query: 1562 EAELHSLVLEREKLSEKLEIIYHHNENLAFGNFENEVGIIVLQNELSNMQENLISTEHKI 1621
            EAEL S++L+REKLSEKLEIIYHHN++L+FG+FENE+  I+LQNELSNMQE LISTEHKI
Sbjct: 1578 EAELQSVLLDREKLSEKLEIIYHHNDHLSFGSFENEIENIILQNELSNMQETLISTEHKI 1637

Query: 1622 VKLEALVSNALQDEDVNDLVSGSS-IEFLDLMVTKLIQNYTASSSGKAVLGKATNGHDTE 1681
            VKLEALVSNAL DED+NDLV GSS IEFL+L+V KL+QNY+ASS G AV G+A NG DTE
Sbjct: 1638 VKLEALVSNALLDEDMNDLVFGSSSIEFLELIVMKLVQNYSASSLGNAVPGRAMNGPDTE 1697

Query: 1682 EEMLARSKNMHDAWQNDINILKKELEDALNQLMVVTEERDQYMEMHESLVVKVESSDKKK 1741
            E MLARS +   AWQND+N+LKK+LEDA++QLMVVT+ERDQYMEMHESL+VKVES DKKK
Sbjct: 1698 E-MLARSIDADVAWQNDVNVLKKDLEDAMHQLMVVTKERDQYMEMHESLIVKVESLDKKK 1757

Query: 1742 DELQELLNLEEQKSASIREKLNVAVRKGKSLIQQRDSLKQAVEEMTTELKHLRSEMKSQE 1801
            DELQELL+LEEQKSAS+REKLNVAVRKGKSL+Q RDS KQA+EEMTTEL+ LRSEMKSQE
Sbjct: 1758 DELQELLSLEEQKSASVREKLNVAVRKGKSLLQLRDSQKQAIEEMTTELEQLRSEMKSQE 1817

Query: 1802 NTLASYEQNFKDFSVYSGRAEALESENLSLKNRLAEIERNFQEKEHKLSSIINTLVHIED 1861
            NTLASYEQ F+DFSVYSGR EALESENLSLKN+L E E N  EKE+KL SIINTL HIE 
Sbjct: 1818 NTLASYEQKFRDFSVYSGRVEALESENLSLKNQLTETESNLGEKEYKLGSIINTLDHIEI 1877

Query: 1862 NVDVSENDPIEKLKQVGKLCSDLREAMYFSEQESVKSRRAAELLLAELNEVQERNDTFQE 1921
            NVDV+E DPIEKLKQVGK+CSDLREAM+ SEQES+KSRRAAELLLAELNEVQERND FQE
Sbjct: 1878 NVDVNETDPIEKLKQVGKMCSDLREAMFLSEQESMKSRRAAELLLAELNEVQERNDAFQE 1937

Query: 1922 ELAKASDEIAELTKERDLAETSKLEALSELEKLSTLHSKEKKIQYSQFMGLKSGLDRLKE 1981
            EL KASDEIAELTKERDLAET+KLEALSELEKLSTLH KE+K Q SQFMGLKSGLDRLKE
Sbjct: 1938 ELVKASDEIAELTKERDLAETTKLEALSELEKLSTLHLKERKNQISQFMGLKSGLDRLKE 1997

Query: 1982 AMREINCLLADAFSKDLDAFYNLEAAIQSCTEPNGPADVNLSHSIVSGAFKSRDLKDKGK 2041
            A+REIN LL DAFS+DLDAFYNLEAAI+SCT+ N P +VN S S VSGAFK    KDKG+
Sbjct: 1998 ALREINGLLVDAFSRDLDAFYNLEAAIESCTKVNVPTEVNPSPSTVSGAFK----KDKGR 2057

Query: 2042 FFSLDSWSNFYTNAHVDENVATEIHS-LVHQLEESMKEIGALKEMIDGHSVSFHKQSDSL 2101
            FF+LDSW N Y NA VDENVA EIHS +VHQLEESMKEIG LKEMIDGH VS HKQSDSL
Sbjct: 2058 FFALDSWLNSYANAPVDENVAMEIHSQVVHQLEESMKEIGGLKEMIDGHCVSIHKQSDSL 2117

Query: 2102 SKILGVLYREVNSQKELVQALKWDVQQSESVAKDREMEGDILCRNIAMLFEACTSVIKEV 2161
            SKILG LY+EVNSQKELVQAL+ +VQQSESVAKD+E EGDILCRN A+L EACTS IKEV
Sbjct: 2118 SKILGELYQEVNSQKELVQALELNVQQSESVAKDKEKEGDILCRNFAVLLEACTSTIKEV 2177

Query: 2162 DERKGELMGHDLTSGNLGMEIISTTPDQLSHAGKTHLLSEESVRKIADRLLLAVREFIGF 2221
            D RKGELMG+DLTS NLG++I+STTPDQLS + KTHLLSEE V+ IADRLLLAVREFI  
Sbjct: 2178 DHRKGELMGNDLTSENLGLDIVSTTPDQLSCSRKTHLLSEEYVQMIADRLLLAVREFISL 2237

Query: 2222 KAEMLDGSLKEMKVSVANLQKELQEKDIQKERICMELVGQIKEAEATASRYSIDLQASKD 2281
            KAEM DGS++EMKV++ANLQKELQEKDIQKE ICMELVGQIKEAE TA+RYS+DLQASKD
Sbjct: 2238 KAEMFDGSVREMKVAMANLQKELQEKDIQKEGICMELVGQIKEAEGTATRYSLDLQASKD 2297

Query: 2282 QVYELEKVTEQMDSERKVLEQRLREMQDGLSISDELRERVRLLTGSLAAKDQEIEALMHA 2341
            +V +LEKV EQ++ ERKVLEQRLREMQDGLSISDELRERVR LT  LAAKDQEIEALMHA
Sbjct: 2298 EVRKLEKVVEQVEGERKVLEQRLREMQDGLSISDELRERVRSLTDLLAAKDQEIEALMHA 2357

Query: 2342 LDEEELQMEGLTKKIEDLERDLKQKNQELESIEASRGKLMKKLSITVTKFDELHHLSESL 2401
            LDEEE+QMEGLT KIE+LE+ LKQKNQELESIE SRGKL KKLSITVTKFDELHHLSESL
Sbjct: 2358 LDEEEVQMEGLTNKIEELEKVLKQKNQELESIETSRGKLTKKLSITVTKFDELHHLSESL 2417

Query: 2402 LTEVEKLQAQLQDRDAEISFLRQEVTRCTNDALVATQTSNRNSEDINEVITWFDTMEARV 2461
            LTEVEKLQAQLQ+RDAEISFLRQEVTRCTNDALVATQTSNR++EDINEVITWFD + AR 
Sbjct: 2418 LTEVEKLQAQLQERDAEISFLRQEVTRCTNDALVATQTSNRSTEDINEVITWFDMVGARA 2477

Query: 2462 GLSHSGHDDKENEVRECKEVLKKKITSILKGIEDLQAESQRKDEMLLAEKNKVEELKRKE 2521
            GLSH GHDD+ENEVRECKEVLKKKITSILK IEDLQA SQRKDE+LL EKNKVEELKR+E
Sbjct: 2478 GLSHIGHDDQENEVRECKEVLKKKITSILKEIEDLQAVSQRKDELLLVEKNKVEELKRRE 2537

Query: 2522 LQLNLLEDVGDNNRASSVAPEIFESEPLHIVKSCILCTLQINKWAASSTSVTPQVRSLRK 2581
            LQLNLLEDVGD+NR SS  PEIFESEPL            INKWAASSTSVTPQVRSLRK
Sbjct: 2538 LQLNLLEDVGDDNRTSSATPEIFESEPL------------INKWAASSTSVTPQVRSLRK 2597

Query: 2582 GNTTDQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRIVPKFSRRMTDMIDGLWVSC 2641
            GNT DQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSR+VPKFSRR TDMIDGLWVSC
Sbjct: 2598 GNT-DQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRLVPKFSRRATDMIDGLWVSC 2657

Query: 2642 DRALMRQPALRLGIIIYWAILHALVATFVV 2643
            DRALMRQPALRLGII YWAILHALVATFVV
Sbjct: 2658 DRALMRQPALRLGIIFYWAILHALVATFVV 2667

BLAST of MC11g0525 vs. NCBI nr
Match: KAG7024516.1 (hypothetical protein SDJN02_13332 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 3826 bits (9923), Expect = 0.0
Identity = 2118/2668 (79.39%), Postives = 2321/2668 (86.99%), Query Frame = 0

Query: 2    LQQFRKKKDNKGTGSQGNSSKNKSKLEQQDADTEIVNVAAKPTSGSYSTDGVLASSFDCD 61
            LQQFRKKKDN+G GSQGNSSKN SKLEQ D D +IV  AAK  SGS STD  L+ S   D
Sbjct: 18   LQQFRKKKDNRGGGSQGNSSKNTSKLEQHDVDADIVTAAAKSPSGSCSTDEALSPSVYRD 77

Query: 62   ADNVDSSASPS-EHSSAAEIDHSTVYLKQEMDLAETSAIDEAEIPVEEVGYREDCDRLIQ 121
             D V SSASPS EHS AAEIDHST  +KQEMDLAETSAID+AE+P++EVGYREDC+  IQ
Sbjct: 78   PDTVYSSASPSMEHSLAAEIDHSTDSVKQEMDLAETSAIDQAEVPMQEVGYREDCEHPIQ 137

Query: 122  NAEAAGVMSSGPSILIDAEVNNNHLCNLSSTESSSQISSASVDEQGRMVEVWSGCREEEI 181
            N EAA  M SG S+  DAE N+NH+CNLSSTESS QISSASV++QGR+ EVW GCREEE+
Sbjct: 138  NTEAA--MPSGLSLPTDAEENDNHICNLSSTESSPQISSASVEQQGRIAEVWGGCREEEL 197

Query: 182  SPARSASLLQAREDVGMTEDASMQSDQVCETQLAEDKQLQTGGMYESAAETTFKDTHCDE 241
             P++SASLLQAREDVGM EDA MQS Q  ET+ + DKQL+TGGM ESAAETTFKD +CD+
Sbjct: 198  LPSQSASLLQAREDVGM-EDALMQSVQAHETEFSGDKQLETGGMNESAAETTFKDRYCDK 257

Query: 242  EEIITVDVASVSGFATVSNDFSISNPGENLGMQSSSSSSRDDWKEERQVHAEDMMHPSMY 301
            EEII  DV SVSG  T SN + IS+PGE L M++SSSSSRDDWKEE QVHAEDM+  S  
Sbjct: 258  EEIIAADVKSVSGADTESNSYLISSPGEKLVMKNSSSSSRDDWKEESQVHAEDMIQSSRC 317

Query: 302  QVQYMPEDNFAVQSEGHERPSQTSAKISEGGDADAIFPNAHMTTDLAVQSGTFYSFGQES 361
            +VQYMPEDNFA QSEGH+  SQ S    + GDA+AI  NAHMT+  +  SGTF SFGQ+S
Sbjct: 318  EVQYMPEDNFADQSEGHDMASQAS----DAGDANAISHNAHMTST-SDASGTFSSFGQDS 377

Query: 362  EFLDLLERVKEELIVTSFSKDISNLQISEQNVLQMELDNQHRKLTNDMSLVNTSLNEILE 421
            +FL LLER+KEELIVTSFSKDI NLQISEQN LQ+ELDN   K TNDM+ +NTSL+E+LE
Sbjct: 378  KFLHLLERMKEELIVTSFSKDIFNLQISEQNELQLELDNHLHKSTNDMTCLNTSLDEVLE 437

Query: 422  RNQSLVDELSQCRSELRDVSSAREELKNQLLTAEAEIENLSSRATESEKNLEKFHADMFR 481
            RNQSLVDELS CRSEL+DVS+ +EEL++QLL AEAEIE LSSR +E+E +LEKFH DMFR
Sbjct: 438  RNQSLVDELSHCRSELKDVSTTKEELRDQLLNAEAEIEKLSSRTSETENSLEKFHGDMFR 497

Query: 482  LSKELDDCKHLVTALEEENERLNGVITSENENKMKLAEEKELYINENEKISSELSSFKSL 541
            L KELDDCKHLVT LEEENERLNG+ITSENENK KLAEEKELYINENEKI SE+SSFKSL
Sbjct: 498  LGKELDDCKHLVTVLEEENERLNGIITSENENKRKLAEEKELYINENEKILSEISSFKSL 557

Query: 542  KVALEDENSKLIGSLSSVAEGKTKLEEEREQLFQVNGTLSVELENCRNLIATQQEEITNL 601
            K+ALE ENSKL+GSLSSV E KTKLEEERE L QVNGTLSVEL NC+NL+ATQQEEIT+L
Sbjct: 558  KMALEVENSKLMGSLSSVVEEKTKLEEEREHLCQVNGTLSVELSNCKNLVATQQEEITDL 617

Query: 602  IKNLALVTEDRTKLEEDKTLLFHENEKMASELLVLDERLSAEYGERVRFEDDLRDALVRL 661
            IKNLAL TEDRTKLEEDK  LFHENEK+ASELLVLDERLS E+ ERV+ E DL+DAL +L
Sbjct: 618  IKNLALATEDRTKLEEDKNRLFHENEKIASELLVLDERLSTEHEERVKLESDLKDALAQL 677

Query: 662  EKLTEENIFLSNSLDIQKSKTEELCGEILPKQMRSIEDGNQTETADSGWHRVNKSQENDA 721
            ++LTEENIFLSN+LDI   K EELC EIL  Q R ++D +Q E  DSG    NK Q ND+
Sbjct: 678  DQLTEENIFLSNNLDIHIFKIEELCSEILSLQTRFVDDEDQAENTDSGRRHGNKFQGNDS 737

Query: 722  YQIK---------------------KQELFDDSFEFITLGRHLEEAYLVLQKLEKEIKGL 781
             QI                      +QE+FDDS   +TLG+HLEEA L+LQKLEKEIKGL
Sbjct: 738  SQITFKENLHETSSVLAGGKPVIVTEQEIFDDSLGLVTLGQHLEEADLMLQKLEKEIKGL 797

Query: 782  QSNSASFSRSGSKVAAPAVSKLIEAFETKVN-EEHEVESEIQLPEDPYKLSSELVDNLGL 841
            QSNSASFS SGSK+AAPAVSKLI+AFE+KVN EE EV++EIQL  DPYKLS+ELVDNL +
Sbjct: 798  QSNSASFSSSGSKMAAPAVSKLIQAFESKVNVEEQEVDAEIQLSNDPYKLSNELVDNLRV 857

Query: 842  LLHQVVVDSENASVLLKGERDHKKVAISTLNKLTDQFEALENHSNDLVIANIDLGVLFEY 901
            LLHQVVVDSE ASVLLKGERDH+KVAISTLN+  DQFE LENHSNDLV+ANI+  +LFE 
Sbjct: 858  LLHQVVVDSEKASVLLKGERDHRKVAISTLNEFKDQFEDLENHSNDLVMANIEHSILFEC 917

Query: 902  LKHHVDDAGGKIYELENLNESLKQQGLYHKISNCELAERLRGYDLKLTELQSQLCDLHQS 961
            LKHHV DAG KIYELE L ESLKQQG++HK SNCELA RL GY LKLTEL+SQLCD HQ 
Sbjct: 918  LKHHVYDAGDKIYELEILKESLKQQGVHHKNSNCELAVRLCGYKLKLTELESQLCDFHQG 977

Query: 962  SNEMVSSTCNQLDKLREGEIARATILEKDLQSFLLELAESIAKLDESLGKSDTAALKFCT 1021
            SNE VS  CNQLD L+EGEI R   LEKD  SFLLELAE+IAKLDESLG S+T+A+KFCT
Sbjct: 978  SNETVSLICNQLDNLQEGEIERGMTLEKDWHSFLLELAETIAKLDESLGNSNTSAIKFCT 1037

Query: 1022 NDQLPSCIIASVIDAVKMIDDLRERLQATGADREAFRMLYEEVNEKYDNLFRRTEFSVDM 1081
            NDQLPSCI  SV +AV +IDDLRERLQAT ++ EAFRMLYEEVNEKYDNLFR TE SVDM
Sbjct: 1038 NDQLPSCIATSVKNAVNIIDDLRERLQATASNGEAFRMLYEEVNEKYDNLFRSTELSVDM 1097

Query: 1082 LHRIYSELQKLYIASCGSVGGSDMNMQIKGLGDPLDYSSFEAFIKLLEDCITERLQLESV 1141
            LHRIY +LQ LYIASCGSV GSDMNMQIK LGDPLDYS+FEA IK LEDCITERL+LES+
Sbjct: 1098 LHRIYGKLQNLYIASCGSVSGSDMNMQIKMLGDPLDYSNFEALIKPLEDCITERLRLESL 1157

Query: 1142 NNKLRLDLEHMNVGFVDFSKRCLDSPGIKKLITDVQSVLLLDDAEMD-VEMPALYLESMV 1201
            N+KLRLDLEH  V FV F +RCLD  GI+KLI +VQSVLLL+D E D  EMPA +LE+MV
Sbjct: 1158 NDKLRLDLEHRTVEFVQFRERCLDPIGIQKLIKNVQSVLLLEDTEKDRAEMPAFHLETMV 1217

Query: 1202 SLLLQKYKETELQLGLSREKSGSLMMKLTELQESVHDLSTLILDHECEIVILKESLSQVQ 1261
            SL+LQKY+E+ELQLGLSRE+ GS+MMKLTELQESVHDLSTLILDHECEIV+LKESLSQ Q
Sbjct: 1218 SLVLQKYRESELQLGLSREECGSVMMKLTELQESVHDLSTLILDHECEIVLLKESLSQAQ 1277

Query: 1262 EALVASRSVLKDKANELEQSEQRVSAIREKLSIAVAKGKGLIVQRDNLKQSLAQTSSELE 1321
            EAL+A RS LKDK +ELEQSEQRVSAIR+KLSIAVAKGKGLIVQRDNLKQ LAQTSSELE
Sbjct: 1278 EALMALRSELKDKVDELEQSEQRVSAIRDKLSIAVAKGKGLIVQRDNLKQLLAQTSSELE 1337

Query: 1322 RCLQELQMKDTRLHETETKLTAYSEAGERVEALESELSYIRNSATALRESFLLKDSVLQR 1381
            RCLQELQMKDTRLHE ETKL  YSEAGERVEALESELSYIRNSATALRESFLLKDSVLQR
Sbjct: 1338 RCLQELQMKDTRLHEVETKLNTYSEAGERVEALESELSYIRNSATALRESFLLKDSVLQR 1397

Query: 1382 IEEILDELDLPENFHSRDIIEKVDWLAKSSTGENLPHTDWDQRSSVAGGSGSDANFVIMD 1441
            IEEILDELDLPENFHSRDIIEK+DWLAKSS GEN+PHTDWDQRSSVAGGSGSDANFVI D
Sbjct: 1398 IEEILDELDLPENFHSRDIIEKIDWLAKSSAGENIPHTDWDQRSSVAGGSGSDANFVITD 1457

Query: 1442 AWKDEVQMDANVGDDLRRKYEELQTRFYGLAEQNEMLEQSLMERNNIVQRWEELLEKIDI 1501
            AWKDEVQ DANVGDDLRRKYEELQT+FYGLAEQNEMLEQSLMERNN VQRWEELLEKIDI
Sbjct: 1458 AWKDEVQPDANVGDDLRRKYEELQTKFYGLAEQNEMLEQSLMERNNAVQRWEELLEKIDI 1517

Query: 1502 PSQLRSMEPEDKMEWLHKSLSEAFHDRDSLHQRVNYLENYCGLLTADLDDSQKKISDLEA 1561
             S LRSMEPEDK+EWL++SLSEA HDRDSLHQRVNYLENYCG LTADLDDS+KKISD+EA
Sbjct: 1518 HSHLRSMEPEDKIEWLNRSLSEACHDRDSLHQRVNYLENYCGSLTADLDDSRKKISDIEA 1577

Query: 1562 ELHSLVLEREKLSEKLEIIYHHNENLAFGNFENEVGIIVLQNELSNMQENLISTEHKIVK 1621
            EL  ++LEREKLSEKLEII +HN++L+FG FENE+  IVLQNELSNMQE LISTE KIVK
Sbjct: 1578 ELQLVLLEREKLSEKLEIIDYHNDHLSFGTFENEIENIVLQNELSNMQEKLISTERKIVK 1637

Query: 1622 LEALVSNALQDEDVNDLVSGSSIEFLDLMVTKLIQNYTASSSGKAVLGKATNGHDTEEEM 1681
            LEALV N LQ+ DV+DLVSGSSIEFL+LMV KL+QNYT  S   AV    TNG  T EEM
Sbjct: 1638 LEALVGNVLQENDVHDLVSGSSIEFLELMVMKLVQNYTTFSLRDAVPESTTNG-STTEEM 1697

Query: 1682 LARSKNMHDAWQNDINILKKELEDALNQLMVVTEERDQYMEMHESLVVKVESSDKKKDEL 1741
            LARS + H AWQNDIN+LKK+LEDA++QLMVVT+ERD+YMEMHE LVVKVES DKKKDEL
Sbjct: 1698 LARSVDAHVAWQNDINVLKKDLEDAMHQLMVVTKERDRYMEMHEYLVVKVESIDKKKDEL 1757

Query: 1742 QELLNLEEQKSASIREKLNVAVRKGKSLIQQRDSLKQAVEEMTTELKHLRSEMKSQENTL 1801
            QELLNLEEQKS SIREKLNVAVRKGKSL+QQRDSLKQA+EEMTTELK+LRSEMKSQENTL
Sbjct: 1758 QELLNLEEQKSTSIREKLNVAVRKGKSLVQQRDSLKQAIEEMTTELKNLRSEMKSQENTL 1817

Query: 1802 ASYEQNFKDFSVYSGRAEALESENLSLKNRLAEIERNFQEKEHKLSSIINTLVHIEDNVD 1861
            ASYEQ  +DFSVY+GR EALESENLSLKN+L E + N QEKE KLSSIINTLVH+E NVD
Sbjct: 1818 ASYEQKLRDFSVYTGRVEALESENLSLKNQLTETKNNLQEKEFKLSSIINTLVHMEVNVD 1877

Query: 1862 VSENDPIEKLKQVGKLCSDLREAMYFSEQESVKSRRAAELLLAELNEVQERNDTFQEELA 1921
            V E DPIEKLKQ+GKLCSDLREAM  SEQESVKSRRAAELLLAELNEVQERND FQEELA
Sbjct: 1878 VYETDPIEKLKQLGKLCSDLREAMVSSEQESVKSRRAAELLLAELNEVQERNDAFQEELA 1937

Query: 1922 KASDEIAELTKERDLAETSKLEALSELEKLSTLHSKEKKIQYSQFMGLKSGLDRLKEAMR 1981
            KASDEIAELTKERDLAETSKLEALSELEKLSTLH KE+K Q+S+FMG KSGLD+LKEA+R
Sbjct: 1938 KASDEIAELTKERDLAETSKLEALSELEKLSTLHLKERKNQFSKFMGFKSGLDQLKEALR 1997

Query: 1982 EINCLLADAFSKDLDAFYNLEAAIQSCTEPNGPADVNLSHSIVSGAFKSRDLKDKGKFFS 2041
            EINCLLADAFS+DLDAFYNLE AI+SCT+ N  A+VN S S VSG  K    KDKG FF+
Sbjct: 1998 EINCLLADAFSRDLDAFYNLEVAIESCTKANDLAEVNPSPSTVSGVVK----KDKGSFFA 2057

Query: 2042 LDSWSNFYTNAHVDENVATEIHSLVHQ-LEESMKEIGALKEMIDGHSVSFHKQSDSLSKI 2101
            LD+W N Y N+ VDENV TEIHS + Q LEES+KEIGALKEMI GHSVSFHK+SDSLSK+
Sbjct: 2058 LDTWLNSYANSPVDENVETEIHSQIMQHLEESIKEIGALKEMIGGHSVSFHKRSDSLSKV 2117

Query: 2102 LGVLYREVNSQKELVQALKWDVQQSESVAKDREMEGDILCRNIAMLFEACTSVIKEVDER 2161
            LG LY+EV SQKELVQAL+ DVQQ ESVAKD+E EGDILCRNIA+L EACTS IKE+D+R
Sbjct: 2118 LGSLYQEVLSQKELVQALELDVQQRESVAKDKEKEGDILCRNIAVLSEACTSTIKEIDQR 2177

Query: 2162 KGELMGHDLTSGNLGMEIISTTPDQLSHAGKTHLLSEESVRKIADRLLLAVREFIGFKAE 2221
            KGELMG+DLTS NLGM+I S TPDQLSH GKTHLLSEE VR+IADRL++ VREFIG KAE
Sbjct: 2178 KGELMGNDLTSENLGMDINSPTPDQLSHIGKTHLLSEEYVRRIADRLVITVREFIGLKAE 2237

Query: 2222 MLDGSLKEMKVSVANLQKELQEKDIQKERICMELVGQIKEAEATASRYSIDLQASKDQVY 2281
            M DG +KEMKV++ANLQKELQEKDIQ ER+CMELVGQIKEAEATA+RYS+DLQASKD++ 
Sbjct: 2238 MFDGHVKEMKVAIANLQKELQEKDIQNERVCMELVGQIKEAEATATRYSLDLQASKDEMR 2297

Query: 2282 ELEKVTEQMDSERKVLEQRLREMQDGLSISDELRERVRLLTGSLAAKDQEIEALMHALDE 2341
            EL+KVTEQM+SERK+LEQRLREM+DGLSISDELRE VR LT SLAAKDQEIEALMHALDE
Sbjct: 2298 ELQKVTEQMESERKILEQRLREMRDGLSISDELRETVRSLTDSLAAKDQEIEALMHALDE 2357

Query: 2342 EELQMEGLTKKIEDLERDLKQKNQELESIEASRGKLMKKLSITVTKFDELHHLSESLLTE 2401
            EE QMEGLT KIE+ E+ LKQKNQELESIE SR KL KKLS+TVTKFDELHHLSESLLTE
Sbjct: 2358 EEEQMEGLTNKIEEQEKVLKQKNQELESIETSRVKLTKKLSLTVTKFDELHHLSESLLTE 2417

Query: 2402 VEKLQAQLQDRDAEISFLRQEVTRCTNDALVATQTSNRNSEDINEVITWFDTMEARVGLS 2461
            VEKLQAQLQDRDAE+SFLRQEVTRCTNDALVATQTSNR++EDINEVITWFD MEARVGLS
Sbjct: 2418 VEKLQAQLQDRDAEVSFLRQEVTRCTNDALVATQTSNRSTEDINEVITWFDMMEARVGLS 2477

Query: 2462 HSGHDDKENEVRECKEVLKKKITSILKGIEDLQAESQRKDEMLLAEKNKVEELKRKELQL 2521
            H GHDD+EN VRECKEVLKKKITSILK IEDLQA SQRKD +LLAEKNKVEELKRKELQL
Sbjct: 2478 HIGHDDQENGVRECKEVLKKKITSILKEIEDLQAVSQRKDALLLAEKNKVEELKRKELQL 2537

Query: 2522 NLLEDVGDNNRASSVAPEIFESEPLHIVKSCILCTL-QINKWAASSTSVTPQVRSLRKGN 2581
            NLLEDVGD NRASS APEIFESEPLH+VK+  LCTL QINKWAASSTSVTPQV SLRKGN
Sbjct: 2538 NLLEDVGDGNRASSAAPEIFESEPLHLVKNSTLCTLAQINKWAASSTSVTPQVPSLRKGN 2597

Query: 2582 TTDQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRIVPKFSRRMTDMIDGLWVSCDR 2641
            T DQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRIVPKFSRR TDMIDGLWVSCDR
Sbjct: 2598 T-DQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRIVPKFSRRATDMIDGLWVSCDR 2657

Query: 2642 ALMRQPALRLGIIIYWAILHALVATFVV 2643
            ALMRQPALRLGII YWAILHAL+ATFVV
Sbjct: 2658 ALMRQPALRLGIIFYWAILHALLATFVV 2671

BLAST of MC11g0525 vs. NCBI nr
Match: XP_023534886.1 (major antigen-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 3805 bits (9868), Expect = 0.0
Identity = 2107/2667 (79.00%), Postives = 2309/2667 (86.58%), Query Frame = 0

Query: 2    LQQFRKKKDNKGTGSQGNSSKNKSKLEQQDADTEIVNVAAKPTSGSYSTDGVLASSFDCD 61
            LQQFRKKKDN+G GSQGNSSKN SKLEQ D D +IV  A K  SGS STD  L+ S   D
Sbjct: 18   LQQFRKKKDNRGGGSQGNSSKNTSKLEQHDVDADIVTAAPKSPSGSCSTDEALSPSVYRD 77

Query: 62   ADNVDSSASPS-EHSSAAEIDHSTVYLKQEMDLAETSAIDEAEIPVEEVGYREDCDRLIQ 121
             D VDSSASPS EHS AAEIDHST  +KQEMDLAETSAID+AE+P++EVGYREDC+  IQ
Sbjct: 78   PDTVDSSASPSMEHSLAAEIDHSTDSVKQEMDLAETSAIDQAEVPMQEVGYREDCEHPIQ 137

Query: 122  NAEAAGVMSSGPSILIDAEVNNNHLCNLSSTESSSQISSASVDEQGRMVEVWSGCREEEI 181
            N EAAG M  G S+  DA+ N+NH+CNLSSTESS QISSASV++QGR+ EVW GCREEE+
Sbjct: 138  NTEAAGFMPFGLSLPTDAQENDNHICNLSSTESSPQISSASVEQQGRIAEVWGGCREEEL 197

Query: 182  SPARSASLLQAREDVGMTEDASMQSDQVCETQLAEDKQLQTGGMYESAAETTFKDTHCDE 241
             P++SASLLQAREDVGM EDA MQS QV ET+ + DKQL+TGGM ESAAETTFKD +CD+
Sbjct: 198  LPSQSASLLQAREDVGM-EDALMQSVQVHETEFSGDKQLETGGMNESAAETTFKDRYCDK 257

Query: 242  EEIITVDVASVSGFATVSNDFSISNPGENLGMQSSSSSSRDDWKEERQVHAEDMMHPSMY 301
            EEII  DV SVSG  T SN + IS+PGE LGM++SSSSSRDDWKEE QVHAEDM+  S  
Sbjct: 258  EEIIAADVKSVSGADTESNSYLISSPGEKLGMKNSSSSSRDDWKEESQVHAEDMIQSSRC 317

Query: 302  QVQYMPEDNFAVQSEGHERPSQTSAKISEGGDADAIFPNAHMTTDLAVQSGTFYSFGQES 361
            +VQYMPEDNFA QSEGH+  SQTS    + GDA+AI  NAHMT+  +  SGT  SFGQ+S
Sbjct: 318  EVQYMPEDNFADQSEGHDMASQTS----DAGDANAISRNAHMTST-SDASGTSSSFGQDS 377

Query: 362  EFLDLLERVKEELIVTSFSKDISNLQISEQNVLQMELDNQHRKLTNDMSLVNTSLNEILE 421
            +FLDLLER+KEELIVTSFSKDI NLQISEQN LQ+ELDN   K TNDM+ +NTSL+E+LE
Sbjct: 378  KFLDLLERMKEELIVTSFSKDIFNLQISEQNELQLELDNHLHKSTNDMTRLNTSLDEVLE 437

Query: 422  RNQSLVDELSQCRSELRDVSSAREELKNQLLTAEAEIENLSSRATESEKNLEKFHADMFR 481
            RNQSLVDELS CRSEL+DVS+ +EEL++QLL AEAEIE LSSR +E+E +LEKFH DMFR
Sbjct: 438  RNQSLVDELSHCRSELKDVSTTKEELRDQLLNAEAEIEKLSSRTSETENSLEKFHGDMFR 497

Query: 482  LSKELDDCKHLVTALEEENERLNGVITSENENKMKLAEEKELYINENEKISSELSSFKSL 541
            L KELDDCKHLVT LEEENERLNG+ITSENENK KLAEEKELY+NENEKI  E+SSFKSL
Sbjct: 498  LGKELDDCKHLVTVLEEENERLNGIITSENENKRKLAEEKELYVNENEKILLEISSFKSL 557

Query: 542  KVALEDENSKLIGSLSSVAEGKTKLEEEREQLFQVNGTLSVELENCRNLIATQQEEITNL 601
            K+ALE ENSKL+GSLSSV E KTKLEEERE L QVNGTLSVEL NC+NL+ATQQEEIT+L
Sbjct: 558  KMALEVENSKLMGSLSSVVEEKTKLEEEREHLCQVNGTLSVELSNCKNLVATQQEEITDL 617

Query: 602  IKNLALVTEDRTKLEEDKTLLFHENEKMASELLVLDERLSAEYGERVRFEDDLRDALVRL 661
            IKNLAL TE RTKLEEDK  LFHENE++ASELLVLDERLS E+ ERV+ E DL+DAL +L
Sbjct: 618  IKNLALATEGRTKLEEDKNRLFHENERIASELLVLDERLSTEHEERVKLESDLKDALAQL 677

Query: 662  EKLTEENIFLSNSLDIQKSKTEELCGEILPKQMRSIEDGNQTETADSGWHRVNKSQENDA 721
            ++LTEENIFLSN+LDI   K EELCGEIL  Q RS++D +Q E  DSG    NKSQ ND+
Sbjct: 678  DQLTEENIFLSNNLDIHIFKIEELCGEILSLQTRSVDDEDQAENTDSGRRHGNKSQGNDS 737

Query: 722  YQIK---------------------KQELFDDSFEFITLGRHLEEAYLVLQKLEKEIKGL 781
             QI                      +QE+FDDS   ITLG+HLEEA L+LQKLEKEI GL
Sbjct: 738  SQITFKENLHETSSVLAGGKPVIVTEQEIFDDSLGLITLGQHLEEADLMLQKLEKEITGL 797

Query: 782  QSNSASFSRSGSKVAAPAVSKLIEAFETKVN-EEHEVESEIQLPEDPYKLSSELVDNLGL 841
            QSNSASFS SGSK+AAPAVSKLI+AFE+KVN EE EV++EIQL  DPYKLS+ELVDNL +
Sbjct: 798  QSNSASFSSSGSKMAAPAVSKLIQAFESKVNVEEQEVDAEIQLSNDPYKLSNELVDNLRV 857

Query: 842  LLHQVVVDSENASVLLKGERDHKKVAISTLNKLTDQFEALENHSNDLVIANIDLGVLFEY 901
            LL QVVVDSE ASVLLKGE DH+KVAISTLN+  DQFE LENHSNDLV+ANI+  +LFE 
Sbjct: 858  LLRQVVVDSEKASVLLKGECDHRKVAISTLNEFKDQFEDLENHSNDLVMANIEHSILFEC 917

Query: 902  LKHHVDDAGGKIYELENLNESLKQQGLYHKISNCELAERLRGYDLKLTELQSQLCDLHQS 961
            LKHHV DAG KIYELE L ESLKQQ ++HK SNCELAERL GY LKLTEL+SQLCD HQ 
Sbjct: 918  LKHHVYDAGDKIYELEILKESLKQQDVHHKNSNCELAERLCGYKLKLTELESQLCDFHQG 977

Query: 962  SNEMVSSTCNQLDKLREGEIARATILEKDLQSFLLELAESIAKLDESLGKSDTAALKFCT 1021
            SNE VS  CNQLD L+EGEI R   LEKD  SFLLELAE+IAKLDESLG S+T+A+KFCT
Sbjct: 978  SNETVSLICNQLDNLQEGEIERGMTLEKDWHSFLLELAETIAKLDESLGNSNTSAIKFCT 1037

Query: 1022 NDQLPSCIIASVIDAVKMIDDLRERLQATGADREAFRMLYEEVNEKYDNLFRRTEFSVDM 1081
            NDQLPSCI  SV +AV MIDD+RERLQAT ++ EAFRMLYEEVNEKYDNLFR TE SVDM
Sbjct: 1038 NDQLPSCIATSVKNAVNMIDDMRERLQATASNGEAFRMLYEEVNEKYDNLFRSTELSVDM 1097

Query: 1082 LHRIYSELQKLYIASCGSVGGSDMNMQIKGLGDPLDYSSFEAFIKLLEDCITERLQLESV 1141
            LHRIY +LQ LYIASCGSV GSDMNMQIK LGDPLDYS+FEA IK LEDCITERL+LES+
Sbjct: 1098 LHRIYGKLQNLYIASCGSVSGSDMNMQIKMLGDPLDYSNFEALIKPLEDCITERLRLESL 1157

Query: 1142 NNKLRLDLEHMNVGFVDFSKRCLDSPGIKKLITDVQSVLLLDDAEMD-VEMPALYLESMV 1201
            N+KLRLDLEH  V FV F +RCLD  GI+KLI +VQSVLLL+D E D  EMPA +LE+MV
Sbjct: 1158 NDKLRLDLEHRTVEFVQFRERCLDPIGIQKLIKNVQSVLLLEDIEKDRAEMPAFHLETMV 1217

Query: 1202 SLLLQKYKETELQLGLSREKSGSLMMKLTELQESVHDLSTLILDHECEIVILKESLSQVQ 1261
            SL+LQKY+E+ELQLGLSRE+ GS+MMKLTELQESVHDLSTLILDHECEIV+LKESLSQ Q
Sbjct: 1218 SLVLQKYRESELQLGLSREECGSVMMKLTELQESVHDLSTLILDHECEIVLLKESLSQAQ 1277

Query: 1262 EALVASRSVLKDKANELEQSEQRVSAIREKLSIAVAKGKGLIVQRDNLKQSLAQTSSELE 1321
            EAL+A RS LKDK +ELEQSE+RVSAIR+KLSIAVAKGKGLIVQRDNLKQ LAQTSSELE
Sbjct: 1278 EALMALRSELKDKVDELEQSEKRVSAIRDKLSIAVAKGKGLIVQRDNLKQLLAQTSSELE 1337

Query: 1322 RCLQELQMKDTRLHETETKLTAYSEAGERVEALESELSYIRNSATALRESFLLKDSVLQR 1381
            RCLQELQMKDTRLHE ETKL  YSEAGERVEALESELSYIRNSATALRESFLLKDSVLQR
Sbjct: 1338 RCLQELQMKDTRLHEVETKLNTYSEAGERVEALESELSYIRNSATALRESFLLKDSVLQR 1397

Query: 1382 IEEILDELDLPENFHSRDIIEKVDWLAKSSTGENLPHTDWDQRSSVAGGSGSDANFVIMD 1441
            IEEILDELDLPENFHSRDIIEK+DWLAKSS GEN+PHTDWDQRSSVAGGSGSDANFVI D
Sbjct: 1398 IEEILDELDLPENFHSRDIIEKIDWLAKSSAGENIPHTDWDQRSSVAGGSGSDANFVITD 1457

Query: 1442 AWKDEVQMDANVGDDLRRKYEELQTRFYGLAEQNEMLEQSLMERNNIVQRWEELLEKIDI 1501
            AWKDEVQ DANVGDDLRRKYEELQT+FYGLAEQNEMLEQSLMERNN VQRWEELLEKIDI
Sbjct: 1458 AWKDEVQPDANVGDDLRRKYEELQTKFYGLAEQNEMLEQSLMERNNAVQRWEELLEKIDI 1517

Query: 1502 PSQLRSMEPEDKMEWLHKSLSEAFHDRDSLHQRVNYLENYCGLLTADLDDSQKKISDLEA 1561
             S LRSMEPEDK+EWL++SLSEA HDRDSLHQRVNYLENYCG LTADLDDS+KKISD+EA
Sbjct: 1518 DSHLRSMEPEDKIEWLNRSLSEACHDRDSLHQRVNYLENYCGSLTADLDDSRKKISDIEA 1577

Query: 1562 ELHSLVLEREKLSEKLEIIYHHNENLAFGNFENEVGIIVLQNELSNMQENLISTEHKIVK 1621
            EL  ++LEREKLSEKLEII HHN++L+FG FENE+   VLQNELSNMQE LISTE KIVK
Sbjct: 1578 ELQLVLLEREKLSEKLEIIDHHNDHLSFGTFENEIENTVLQNELSNMQEKLISTERKIVK 1637

Query: 1622 LEALVSNALQDEDVNDLVSGSSIEFLDLMVTKLIQNYTASSSGKAVLGKATNGHDTEEEM 1681
            LEALV N LQD DV+ LVSGSSIEFL+LMV KL+QNYT  S   AV    TNG +TEE M
Sbjct: 1638 LEALVGNVLQDNDVHGLVSGSSIEFLELMVMKLVQNYTTFSLRDAVPESTTNGSNTEE-M 1697

Query: 1682 LARSKNMHDAWQNDINILKKELEDALNQLMVVTEERDQYMEMHESLVVKVESSDKKKDEL 1741
            LARS + H AWQNDIN+LKK+LEDA++QLMVVT+ERD+YMEMHE LVVKVES DKKKDEL
Sbjct: 1698 LARSVDAHVAWQNDINVLKKDLEDAMHQLMVVTKERDRYMEMHEYLVVKVESIDKKKDEL 1757

Query: 1742 QELLNLEEQKSASIREKLNVAVRKGKSLIQQRDSLKQAVEEMTTELKHLRSEMKSQENTL 1801
            QELLNLEEQKS SIREKLNVAVRKGKSL+QQRDSLKQA+EEMTTELK+LRSEMKSQENTL
Sbjct: 1758 QELLNLEEQKSTSIREKLNVAVRKGKSLVQQRDSLKQAIEEMTTELKNLRSEMKSQENTL 1817

Query: 1802 ASYEQNFKDFSVYSGRAEALESENLSLKNRLAEIERNFQEKEHKLSSIINTLVHIEDNVD 1861
            ASYEQ  +DFSVY+GR EALESENLSLKN+L E + N QEKE KLSSIINTLVH+E NVD
Sbjct: 1818 ASYEQKLRDFSVYTGRVEALESENLSLKNQLTETKNNLQEKEFKLSSIINTLVHMEVNVD 1877

Query: 1862 VSENDPIEKLKQVGKLCSDLREAMYFSEQESVKSRRAAELLLAELNEVQERNDTFQEELA 1921
            V E DPIEKLKQ+GKLCSDLREAM  SEQESVKSRRAAELLLAELNEVQERND FQEELA
Sbjct: 1878 VYETDPIEKLKQLGKLCSDLREAMVSSEQESVKSRRAAELLLAELNEVQERNDAFQEELA 1937

Query: 1922 KASDEIAELTKERDLAETSKLEALSELEKLSTLHSKEKKIQYSQFMGLKSGLDRLKEAMR 1981
            KASDEIAELTKERDLAETSKLEALSELEKLSTLH KE+K Q+S+FMG KSGLD+LKE +R
Sbjct: 1938 KASDEIAELTKERDLAETSKLEALSELEKLSTLHLKERKNQFSKFMGFKSGLDQLKETLR 1997

Query: 1982 EINCLLADAFSKDLDAFYNLEAAIQSCTEPNGPADVNLSHSIVSGAFKSRDLKDKGKFFS 2041
            EINCLLADAFS+DLDAFYNLE AI+SCT+ N  A+VN S S VSG  K    KDKG FF+
Sbjct: 1998 EINCLLADAFSRDLDAFYNLEVAIESCTKANDLAEVNPSPSTVSGVVK----KDKGSFFA 2057

Query: 2042 LDSWSNFYTNAHVDENVATEIHSLVHQ-LEESMKEIGALKEMIDGHSVSFHKQSDSLSKI 2101
            LD+W N Y N+ VDEN  TEIHS + Q LEES+KEIGALKEMI GHSVSFHK+SDSLSK+
Sbjct: 2058 LDTWLNSYANSPVDENAETEIHSQIMQHLEESIKEIGALKEMIGGHSVSFHKRSDSLSKV 2117

Query: 2102 LGVLYREVNSQKELVQALKWDVQQSESVAKDREMEGDILCRNIAMLFEACTSVIKEVDER 2161
            LG L++EV SQKELVQAL+ DVQQ ESVAKD+E EGDILCRNIA+L EACTS IKE+D+R
Sbjct: 2118 LGSLHQEVLSQKELVQALELDVQQRESVAKDKEKEGDILCRNIAVLSEACTSTIKEIDQR 2177

Query: 2162 KGELMGHDLTSGNLGMEIISTTPDQLSHAGKTHLLSEESVRKIADRLLLAVREFIGFKAE 2221
            KGELMG+DLTS NLGM+I S TPDQLSH GKTHLLSEE VR+IADRLL+ VREFIG KAE
Sbjct: 2178 KGELMGNDLTSENLGMDINSPTPDQLSHIGKTHLLSEEYVRRIADRLLITVREFIGLKAE 2237

Query: 2222 MLDGSLKEMKVSVANLQKELQEKDIQKERICMELVGQIKEAEATASRYSIDLQASKDQVY 2281
            M DG +KEMKV++ANLQKELQEKDIQ ER+CMELVGQIKEAEATA+RYS+DLQASKD++ 
Sbjct: 2238 MFDGHVKEMKVAIANLQKELQEKDIQNERVCMELVGQIKEAEATATRYSLDLQASKDEMR 2297

Query: 2282 ELEKVTEQMDSERKVLEQRLREMQDGLSISDELRERVRLLTGSLAAKDQEIEALMHALDE 2341
            EL+KVTEQM+SERK+LEQRLREM+DGLSISDELRE VR LT SLAAKDQEIEALMHALDE
Sbjct: 2298 ELQKVTEQMESERKILEQRLREMRDGLSISDELRETVRSLTDSLAAKDQEIEALMHALDE 2357

Query: 2342 EELQMEGLTKKIEDLERDLKQKNQELESIEASRGKLMKKLSITVTKFDELHHLSESLLTE 2401
            EE QMEGLT KIE  E+ LKQKNQELESIE SRGKL KKLS+TVTKFDELHHLSESLLTE
Sbjct: 2358 EEEQMEGLTNKIEAQEKVLKQKNQELESIETSRGKLTKKLSLTVTKFDELHHLSESLLTE 2417

Query: 2402 VEKLQAQLQDRDAEISFLRQEVTRCTNDALVATQTSNRNSEDINEVITWFDTMEARVGLS 2461
            VEKLQAQLQDRDAE+SFLRQEVTRCTNDALVATQTSNR++EDINEVITWFD MEARVGLS
Sbjct: 2418 VEKLQAQLQDRDAEVSFLRQEVTRCTNDALVATQTSNRSTEDINEVITWFDMMEARVGLS 2477

Query: 2462 HSGHDDKENEVRECKEVLKKKITSILKGIEDLQAESQRKDEMLLAEKNKVEELKRKELQL 2521
            H GHDD+EN VRECKEVLKKKITSILK IEDLQA SQRKD +LLAEKNKVEELKRKELQL
Sbjct: 2478 HIGHDDQENGVRECKEVLKKKITSILKEIEDLQAVSQRKDALLLAEKNKVEELKRKELQL 2537

Query: 2522 NLLEDVGDNNRASSVAPEIFESEPLHIVKSCILCTLQINKWAASSTSVTPQVRSLRKGNT 2581
            NLLEDVGD NRASS APEIFESEPL            INKWAASSTSVTPQV SLRKGNT
Sbjct: 2538 NLLEDVGDGNRASSAAPEIFESEPL------------INKWAASSTSVTPQVPSLRKGNT 2597

Query: 2582 TDQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRIVPKFSRRMTDMIDGLWVSCDRA 2641
             DQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRIVPKFSRR TDMIDGLWVSCDRA
Sbjct: 2598 -DQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRIVPKFSRRATDMIDGLWVSCDRA 2657

Query: 2642 LMRQPALRLGIIIYWAILHALVATFVV 2643
            LMRQPALRLGII YWAILHAL+ATFVV
Sbjct: 2658 LMRQPALRLGIIFYWAILHALLATFVV 2660

BLAST of MC11g0525 vs. NCBI nr
Match: XP_022975256.1 (golgin subfamily A member 4-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 3796 bits (9843), Expect = 0.0
Identity = 2097/2667 (78.63%), Postives = 2307/2667 (86.50%), Query Frame = 0

Query: 2    LQQFRKKKDNKGTGSQGNSSKNKSKLEQQDADTEIVNVAAKPTSGSYSTDGVLASSFDCD 61
            LQQFRKKKDN+G GSQGNSSKN SKLEQ D D +IV  AAK  SGS STD  L+ S   +
Sbjct: 18   LQQFRKKKDNRGGGSQGNSSKNTSKLEQHDVDADIVTAAAKSPSGSCSTDEALSPSVYRN 77

Query: 62   ADNVDSSASPS-EHSSAAEIDHSTVYLKQEMDLAETSAIDEAEIPVEEVGYREDCDRLIQ 121
             D VDSSASPS EHS AAEIDHST  +KQEMDLAETSAID+AE+P++E+ YREDC+  IQ
Sbjct: 78   PDTVDSSASPSMEHSLAAEIDHSTDSVKQEMDLAETSAIDQAEVPMQEIAYREDCEHPIQ 137

Query: 122  NAEAAGVMSSGPSILIDAEVNNNHLCNLSSTESSSQISSASVDEQGRMVEVWSGCREEEI 181
            N EAAG M  G S+  DAE N+NH+CNLSSTESS QISS SV++QGR+ EVW GCREEE+
Sbjct: 138  NTEAAGFMPFGLSLPTDAEENDNHICNLSSTESSPQISSPSVEQQGRIAEVWGGCREEEL 197

Query: 182  SPARSASLLQAREDVGMTEDASMQSDQVCETQLAEDKQLQTGGMYESAAETTFKDTHCDE 241
             P++SASLLQAREDVGM EDA +QS QV ET+ + DK+L+TGGM ESAAETTFKD +CD+
Sbjct: 198  LPSQSASLLQAREDVGM-EDALVQSVQVHETEFSGDKKLETGGMNESAAETTFKDRYCDK 257

Query: 242  EEIITVDVASVSGFATVSNDFSISNPGENLGMQSSSSSSRDDWKEERQVHAEDMMHPSMY 301
            EEII VDV SVSG  T SN + IS+PGE LGM++SSSSSRDDWKEE Q HAEDM+  S  
Sbjct: 258  EEIIAVDVKSVSGADTESNSYLISSPGEKLGMKNSSSSSRDDWKEESQAHAEDMIQSSRC 317

Query: 302  QVQYMPEDNFAVQSEGHERPSQTSAKISEGGDADAIFPNAHMTTDLAVQSGTFYSFGQES 361
            +VQYMPEDNFA QSEGH+  SQTS    + GDA+AI  NAHMT+ L   SGTF SFGQ+S
Sbjct: 318  EVQYMPEDNFADQSEGHDMASQTS----DAGDANAISHNAHMTSTLDA-SGTFSSFGQDS 377

Query: 362  EFLDLLERVKEELIVTSFSKDISNLQISEQNVLQMELDNQHRKLTNDMSLVNTSLNEILE 421
            +FLDLLER+KEELIVTSFSKDI NLQI EQN LQ+ELDN   K TNDM+ +NTSLNE+LE
Sbjct: 378  KFLDLLERMKEELIVTSFSKDIFNLQIYEQNELQLELDNHLHKSTNDMTRLNTSLNEVLE 437

Query: 422  RNQSLVDELSQCRSELRDVSSAREELKNQLLTAEAEIENLSSRATESEKNLEKFHADMFR 481
            RNQSLV+ELS CRSEL+DVS+ +EEL+N LL AEAEIE LS+R +E+E +LEKFH DMFR
Sbjct: 438  RNQSLVNELSHCRSELKDVSTTKEELRNHLLNAEAEIEKLSTRTSETENSLEKFHGDMFR 497

Query: 482  LSKELDDCKHLVTALEEENERLNGVITSENENKMKLAEEKELYINENEKISSELSSFKSL 541
            L  ELDDCKHLVT LEEENERLNG+ITSENENK KLAEEKELYINENEKI  E+SSFKSL
Sbjct: 498  LGNELDDCKHLVTVLEEENERLNGIITSENENKRKLAEEKELYINENEKILLEISSFKSL 557

Query: 542  KVALEDENSKLIGSLSSVAEGKTKLEEEREQLFQVNGTLSVELENCRNLIATQQEEITNL 601
            K+ALE ENSKL+GS SSV E KTKLEEERE L QVNGTLSVEL NC+NL+ATQQEEIT+L
Sbjct: 558  KMALEVENSKLMGSFSSVVEEKTKLEEEREHLCQVNGTLSVELSNCKNLVATQQEEITDL 617

Query: 602  IKNLALVTEDRTKLEEDKTLLFHENEKMASELLVLDERLSAEYGERVRFEDDLRDALVRL 661
            IKNLAL TEDRTKLEEDK  LFHENE++ASELLVLDERLS E+ ERV+ E DL+DAL +L
Sbjct: 618  IKNLALATEDRTKLEEDKNRLFHENERIASELLVLDERLSTEHVERVKLESDLKDALAQL 677

Query: 662  EKLTEENIFLSNSLDIQKSKTEELCGEILPKQMRSIEDGNQTETADSGWHRVNKSQENDA 721
            ++LTEENIFLSN+LDI   K EELCGEIL  Q RS++D +Q E  DSG    NKSQ ND+
Sbjct: 678  DQLTEENIFLSNNLDIHIFKIEELCGEILSLQTRSVDDEDQAENTDSGRRHGNKSQGNDS 737

Query: 722  YQIK---------------------KQELFDDSFEFITLGRHLEEAYLVLQKLEKEIKGL 781
             QI                      +QE+FDDS E +TLG+HLEEA L+LQKLE+EI GL
Sbjct: 738  SQITFKENLHETSSVLAGGKPVIVTEQEIFDDSLELVTLGQHLEEADLMLQKLEREITGL 797

Query: 782  QSNSASFSRSGSKVAAPAVSKLIEAFETKVN-EEHEVESEIQLPEDPYKLSSELVDNLGL 841
            QSNSASFS SGSK+AAPA+SKLI+AFE+KVN EE EV++E QL  DPYKLS+ELVDNL +
Sbjct: 798  QSNSASFSSSGSKMAAPAISKLIQAFESKVNVEEQEVDAETQLSNDPYKLSNELVDNLRV 857

Query: 842  LLHQVVVDSENASVLLKGERDHKKVAISTLNKLTDQFEALENHSNDLVIANIDLGVLFEY 901
            LL QVVVDSE ASVLLKGERDH+KVAISTLNK  DQFE LENHSNDLV+ANI+  +LFE 
Sbjct: 858  LLRQVVVDSEKASVLLKGERDHRKVAISTLNKFKDQFEDLENHSNDLVMANIEHSILFEC 917

Query: 902  LKHHVDDAGGKIYELENLNESLKQQGLYHKISNCELAERLRGYDLKLTELQSQLCDLHQS 961
            LKHHV  AG KIYELE L ESLKQQG++HK SNCELAERL GY LKLTEL+SQLCD HQ 
Sbjct: 918  LKHHVYGAGDKIYELEILKESLKQQGVHHKNSNCELAERLCGYKLKLTELESQLCDFHQG 977

Query: 962  SNEMVSSTCNQLDKLREGEIARATILEKDLQSFLLELAESIAKLDESLGKSDTAALKFCT 1021
            SNE VS  CNQLD L+EGEI R   LEKD  SFLLELAE+IAKLDESLG S+T+A+KFCT
Sbjct: 978  SNETVSLICNQLDNLQEGEIERGMTLEKDWHSFLLELAETIAKLDESLGNSNTSAIKFCT 1037

Query: 1022 NDQLPSCIIASVIDAVKMIDDLRERLQATGADREAFRMLYEEVNEKYDNLFRRTEFSVDM 1081
            NDQLPSCI  SV +AV MIDDLRERLQAT +  EAFRMLYEEVNEKYDNLFR TE SVDM
Sbjct: 1038 NDQLPSCIATSVKNAVNMIDDLRERLQATASHGEAFRMLYEEVNEKYDNLFRSTELSVDM 1097

Query: 1082 LHRIYSELQKLYIASCGSVGGSDMNMQIKGLGDPLDYSSFEAFIKLLEDCITERLQLESV 1141
            LHRIY +LQ LYIASCGSV GSDMNMQ+K LGDPLDYS+FEA IK LEDCITERL+LES+
Sbjct: 1098 LHRIYGKLQNLYIASCGSVSGSDMNMQMKMLGDPLDYSNFEALIKPLEDCITERLRLESL 1157

Query: 1142 NNKLRLDLEHMNVGFVDFSKRCLDSPGIKKLITDVQSVLLLDDAEMD-VEMPALYLESMV 1201
            N+KLRLDLEH  V FV F +RCLD  GI+KLI +VQSVLLL+D E D  EMPA +LE+MV
Sbjct: 1158 NDKLRLDLEHRTVEFVQFRERCLDPIGIQKLIKNVQSVLLLEDTEKDRAEMPAFHLETMV 1217

Query: 1202 SLLLQKYKETELQLGLSREKSGSLMMKLTELQESVHDLSTLILDHECEIVILKESLSQVQ 1261
            SL+LQKY+E+ELQLGLSRE+ GS+M+KLTELQESVHDLSTLILDHECEIV+LKESLSQ Q
Sbjct: 1218 SLVLQKYRESELQLGLSREECGSVMIKLTELQESVHDLSTLILDHECEIVLLKESLSQAQ 1277

Query: 1262 EALVASRSVLKDKANELEQSEQRVSAIREKLSIAVAKGKGLIVQRDNLKQSLAQTSSELE 1321
            EAL+A RS LKDK +ELEQSEQRVSAIR+KLSIAVAKGKGLIVQRDNLKQ LAQTSSELE
Sbjct: 1278 EALMALRSELKDKVDELEQSEQRVSAIRDKLSIAVAKGKGLIVQRDNLKQLLAQTSSELE 1337

Query: 1322 RCLQELQMKDTRLHETETKLTAYSEAGERVEALESELSYIRNSATALRESFLLKDSVLQR 1381
            RCLQELQMKDTRLHE ETKL  YSEAGERVEALESELSYIRNSATALRESFLLKDSVLQR
Sbjct: 1338 RCLQELQMKDTRLHEVETKLNTYSEAGERVEALESELSYIRNSATALRESFLLKDSVLQR 1397

Query: 1382 IEEILDELDLPENFHSRDIIEKVDWLAKSSTGENLPHTDWDQRSSVAGGSGSDANFVIMD 1441
            IEEILDELDLPENFHSRDIIEK+DWLAKSS GEN+PHTDWDQRSSVAGGSGSDANFVI D
Sbjct: 1398 IEEILDELDLPENFHSRDIIEKIDWLAKSSAGENIPHTDWDQRSSVAGGSGSDANFVITD 1457

Query: 1442 AWKDEVQMDANVGDDLRRKYEELQTRFYGLAEQNEMLEQSLMERNNIVQRWEELLEKIDI 1501
            AWKDEVQ DANVGDDLRRKYEELQ++FYGLAEQNEMLEQSLMERNN VQRWEELLEKIDI
Sbjct: 1458 AWKDEVQPDANVGDDLRRKYEELQSKFYGLAEQNEMLEQSLMERNNAVQRWEELLEKIDI 1517

Query: 1502 PSQLRSMEPEDKMEWLHKSLSEAFHDRDSLHQRVNYLENYCGLLTADLDDSQKKISDLEA 1561
             S LRSMEPEDK+EWLH+SLSEA HDRDSLHQRVNYLENYCG LTADLDDS+KKISD+E+
Sbjct: 1518 HSHLRSMEPEDKIEWLHRSLSEACHDRDSLHQRVNYLENYCGSLTADLDDSRKKISDIES 1577

Query: 1562 ELHSLVLEREKLSEKLEIIYHHNENLAFGNFENEVGIIVLQNELSNMQENLISTEHKIVK 1621
            EL  ++LEREKLSEKLEII HHN++L+FG+FENE+  IVLQNELSNMQE LISTE KI+K
Sbjct: 1578 ELQLVLLEREKLSEKLEIIDHHNDHLSFGSFENEIENIVLQNELSNMQEKLISTERKILK 1637

Query: 1622 LEALVSNALQDEDVNDLVSGSSIEFLDLMVTKLIQNYTASSSGKAVLGKATNGHDTEEEM 1681
            LEALV N LQD DV+DLVSG+SIEF +LMV KL+QNYT  S   AV    TNG +TEE M
Sbjct: 1638 LEALVGNVLQDNDVHDLVSGNSIEFFELMVMKLVQNYTTFSLRDAVPESTTNGSNTEE-M 1697

Query: 1682 LARSKNMHDAWQNDINILKKELEDALNQLMVVTEERDQYMEMHESLVVKVESSDKKKDEL 1741
            LARS + H AWQNDIN+LKK+LEDA++QLMVVT+ERD+YMEMHE LVVKVES DKKKDEL
Sbjct: 1698 LARSVDAHVAWQNDINVLKKDLEDAMHQLMVVTKERDRYMEMHEYLVVKVESIDKKKDEL 1757

Query: 1742 QELLNLEEQKSASIREKLNVAVRKGKSLIQQRDSLKQAVEEMTTELKHLRSEMKSQENTL 1801
            QELLNLEEQKS SIREKLNVAVRKGKSL+QQRDSLKQA+EEMTTELK+LRSEMKSQEN L
Sbjct: 1758 QELLNLEEQKSTSIREKLNVAVRKGKSLVQQRDSLKQAIEEMTTELKNLRSEMKSQENIL 1817

Query: 1802 ASYEQNFKDFSVYSGRAEALESENLSLKNRLAEIERNFQEKEHKLSSIINTLVHIEDNVD 1861
            ASYEQ  KDFSVY+GR EALESENLSLKN+L E + N QEKE KLSSIINTLVH+E NVD
Sbjct: 1818 ASYEQKLKDFSVYTGRVEALESENLSLKNQLTETKNNLQEKEFKLSSIINTLVHMEVNVD 1877

Query: 1862 VSENDPIEKLKQVGKLCSDLREAMYFSEQESVKSRRAAELLLAELNEVQERNDTFQEELA 1921
            V E DPIEKLKQ+GKLCSDLREAM  SEQES KSRRAAELLLAELNEVQERND FQEELA
Sbjct: 1878 VYETDPIEKLKQLGKLCSDLREAMVSSEQESAKSRRAAELLLAELNEVQERNDAFQEELA 1937

Query: 1922 KASDEIAELTKERDLAETSKLEALSELEKLSTLHSKEKKIQYSQFMGLKSGLDRLKEAMR 1981
            KASDEIAELTKERDLAETSKLEALSELEKLSTLH KE+K Q+S+FMG KSGLD+LKEA+R
Sbjct: 1938 KASDEIAELTKERDLAETSKLEALSELEKLSTLHLKERKNQFSKFMGFKSGLDQLKEALR 1997

Query: 1982 EINCLLADAFSKDLDAFYNLEAAIQSCTEPNGPADVNLSHSIVSGAFKSRDLKDKGKFFS 2041
            EINCLLADAFS+DLDAFYNLE AI+SCT+ N  A+VN S S VSG  K    KDKG FF+
Sbjct: 1998 EINCLLADAFSRDLDAFYNLEVAIESCTKANDLAEVNPSPSTVSGVVK----KDKGSFFA 2057

Query: 2042 LDSWSNFYTNAHVDENVATEIHSLVHQ-LEESMKEIGALKEMIDGHSVSFHKQSDSLSKI 2101
            LD+  N Y N+ VDENV TEIHS + Q LEES+KEIGALKEMI GHSVSFHK+SDSLSK+
Sbjct: 2058 LDALLNSYANSPVDENVETEIHSQIMQHLEESIKEIGALKEMIGGHSVSFHKRSDSLSKV 2117

Query: 2102 LGVLYREVNSQKELVQALKWDVQQSESVAKDREMEGDILCRNIAMLFEACTSVIKEVDER 2161
            LG LY+EV SQKELVQAL+ DVQQ ESVAKD+E EGDILCRNIA+L EACTS IKE+++R
Sbjct: 2118 LGSLYQEVLSQKELVQALELDVQQRESVAKDKEKEGDILCRNIAVLSEACTSTIKEINQR 2177

Query: 2162 KGELMGHDLTSGNLGMEIISTTPDQLSHAGKTHLLSEESVRKIADRLLLAVREFIGFKAE 2221
            KGELMG+DLTS NLGM+I S TPDQLSH GKTHLLSEE V +IADRLL+ VREFIG KAE
Sbjct: 2178 KGELMGNDLTSENLGMDINSPTPDQLSHIGKTHLLSEEYVGRIADRLLITVREFIGLKAE 2237

Query: 2222 MLDGSLKEMKVSVANLQKELQEKDIQKERICMELVGQIKEAEATASRYSIDLQASKDQVY 2281
            M DG +KEMKV++ANLQKELQEKDIQ ER+CMELVGQIKEAEATA+RYS+DLQASKD++ 
Sbjct: 2238 MFDGHVKEMKVAIANLQKELQEKDIQNERVCMELVGQIKEAEATATRYSLDLQASKDEMR 2297

Query: 2282 ELEKVTEQMDSERKVLEQRLREMQDGLSISDELRERVRLLTGSLAAKDQEIEALMHALDE 2341
            EL+KVTEQM+SERK+LEQRLREM+DGLSISDELRE VR LT SLAAKDQEIEALMHALDE
Sbjct: 2298 ELQKVTEQMESERKILEQRLREMRDGLSISDELRETVRSLTDSLAAKDQEIEALMHALDE 2357

Query: 2342 EELQMEGLTKKIEDLERDLKQKNQELESIEASRGKLMKKLSITVTKFDELHHLSESLLTE 2401
            EE QMEGLT KIE+ E+ LKQKNQELESIE SRGKL KKLS+TVTKFDELHHLSESLLTE
Sbjct: 2358 EEEQMEGLTNKIEEKEKVLKQKNQELESIETSRGKLTKKLSLTVTKFDELHHLSESLLTE 2417

Query: 2402 VEKLQAQLQDRDAEISFLRQEVTRCTNDALVATQTSNRNSEDINEVITWFDTMEARVGLS 2461
            VEKLQAQLQDRDAE+SFLRQEVTRCTNDALVATQTSNR++EDINEVITW D MEARVGLS
Sbjct: 2418 VEKLQAQLQDRDAEVSFLRQEVTRCTNDALVATQTSNRSTEDINEVITWVDMMEARVGLS 2477

Query: 2462 HSGHDDKENEVRECKEVLKKKITSILKGIEDLQAESQRKDEMLLAEKNKVEELKRKELQL 2521
            H GHDD+EN++RECKEVLKKKITSILK IEDLQA SQRKD +LLAEKNKVEELKRKELQL
Sbjct: 2478 HIGHDDQENDIRECKEVLKKKITSILKEIEDLQAVSQRKDALLLAEKNKVEELKRKELQL 2537

Query: 2522 NLLEDVGDNNRASSVAPEIFESEPLHIVKSCILCTLQINKWAASSTSVTPQVRSLRKGNT 2581
            NLLEDVGD NRASS APEIFESEPL            INKWAASSTSVTPQV SLRKGNT
Sbjct: 2538 NLLEDVGDGNRASSAAPEIFESEPL------------INKWAASSTSVTPQVPSLRKGNT 2597

Query: 2582 TDQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRIVPKFSRRMTDMIDGLWVSCDRA 2641
             DQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRIVPKFSRR TDMIDGLWVSCDRA
Sbjct: 2598 -DQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRIVPKFSRRATDMIDGLWVSCDRA 2657

Query: 2642 LMRQPALRLGIIIYWAILHALVATFVV 2643
            LMRQPALRLGII YWAILHAL+ATFVV
Sbjct: 2658 LMRQPALRLGIIFYWAILHALLATFVV 2660

BLAST of MC11g0525 vs. ExPASy TrEMBL
Match: A0A6J1BYX1 (centromere-associated protein E OS=Momordica charantia OX=3673 GN=LOC111005948 PE=4 SV=1)

HSP 1 Score: 4865 bits (12620), Expect = 0.0
Identity = 2630/2698 (97.48%), Postives = 2630/2698 (97.48%), Query Frame = 0

Query: 2    LQQFRKKKDNKGTGSQGNSSKNKSKLEQQDADTEIVNVAAKPTSGSYSTDGVLASSFDCD 61
            LQQFRKKKDNKGTGSQGNSSKNKSKLEQQDADTEIVNVAAKPTSGSYSTDGVLASSFDCD
Sbjct: 18   LQQFRKKKDNKGTGSQGNSSKNKSKLEQQDADTEIVNVAAKPTSGSYSTDGVLASSFDCD 77

Query: 62   ADNVDSSASPSEHSSAAEIDHSTVYLKQEMDLAETSAIDEAEIPVEEVGYREDCDRLIQN 121
            ADNVDSSASPSEHSSAAEIDHSTVYLKQEMDLAETSAIDEAEIPVEEVGYREDCDRLIQN
Sbjct: 78   ADNVDSSASPSEHSSAAEIDHSTVYLKQEMDLAETSAIDEAEIPVEEVGYREDCDRLIQN 137

Query: 122  AEAAGVMSSGPSILIDAEVNNNHLCNLSSTESSSQISSASVDEQGRMVEVWSGCREEEIS 181
            AEAAGVMSSGPSILIDAEVNNNHLCNLSSTESSSQISSASVDEQGRMVEVWSGCREEEIS
Sbjct: 138  AEAAGVMSSGPSILIDAEVNNNHLCNLSSTESSSQISSASVDEQGRMVEVWSGCREEEIS 197

Query: 182  PARSASLLQAREDVGMTEDASMQSDQVCETQLAEDKQLQTGGMYESAAETTFKDTHCDEE 241
            PARSASLLQAREDVGMTEDASMQSDQVCETQLAEDKQLQTGGMYESAAETTFKDTHCDEE
Sbjct: 198  PARSASLLQAREDVGMTEDASMQSDQVCETQLAEDKQLQTGGMYESAAETTFKDTHCDEE 257

Query: 242  EIITVDVASVSGFATVSNDFSISNPGENLGMQSSSSSSRDDWKEERQVHAEDMMHPSMYQ 301
            EIITVDVASVSGFATVSNDFSISNPGENLGMQSSSSSSRDDWKEERQVHAEDMMHPSMYQ
Sbjct: 258  EIITVDVASVSGFATVSNDFSISNPGENLGMQSSSSSSRDDWKEERQVHAEDMMHPSMYQ 317

Query: 302  VQYMPEDNFAVQSEGHERPSQTSAKISEGGDADAIFPNAHMTTDLAVQSGTFYSFGQESE 361
            VQYMPEDNFAVQSEGHERPSQTSAKISEGGDADAIFPNAHMTTDLAVQSGTFYSFGQESE
Sbjct: 318  VQYMPEDNFAVQSEGHERPSQTSAKISEGGDADAIFPNAHMTTDLAVQSGTFYSFGQESE 377

Query: 362  FLDLLERVKEELIVTSFSKDISNLQISEQNVLQMELDNQHRKLTNDMSLVNTSLNEILER 421
            FLDLLERVKEELIVTSFSKDISNLQISEQNVLQMELDNQHRKLTNDMSLVNTSLNEILER
Sbjct: 378  FLDLLERVKEELIVTSFSKDISNLQISEQNVLQMELDNQHRKLTNDMSLVNTSLNEILER 437

Query: 422  NQSLVDELSQCRSELRDVSSAREELKNQLLTAEAEIENLSSRATESEKNLEKFHADMFRL 481
            NQSLVDELSQCRSELRDVSSAREELKNQLLTAEAEIENLSSRATESEKNLEKFHADMFRL
Sbjct: 438  NQSLVDELSQCRSELRDVSSAREELKNQLLTAEAEIENLSSRATESEKNLEKFHADMFRL 497

Query: 482  SKELDDCKHLVTALEEENERLNGVITSENENKMKLAEEKELYINENEKISSELSSFKSLK 541
            SKELDDCKHLVTALEEENERLNGVITSENENKMKLAEEKELYINENEKISSELSSFKSLK
Sbjct: 498  SKELDDCKHLVTALEEENERLNGVITSENENKMKLAEEKELYINENEKISSELSSFKSLK 557

Query: 542  VALEDENSKLIGSLSSVAEGKTKLEEEREQLFQVNGTLSVELENCRNLIATQQEEITNLI 601
            VALEDENSKLIGSLSSVAEGKTKLEEEREQLFQVNGTLSVELENCRNLIATQQEEITNLI
Sbjct: 558  VALEDENSKLIGSLSSVAEGKTKLEEEREQLFQVNGTLSVELENCRNLIATQQEEITNLI 617

Query: 602  KNLALVTEDRTKLEEDKTLLFHENEKMASELLVLDERLSAEYGERVRFEDDLRDALVRLE 661
            KNLALVTEDRTKLEEDKTLLFHENEKMASELLVLDERLSAEYGERVRFEDDLRDALVRLE
Sbjct: 618  KNLALVTEDRTKLEEDKTLLFHENEKMASELLVLDERLSAEYGERVRFEDDLRDALVRLE 677

Query: 662  KLTEENIFLSNSLDIQKSKTEELCGEILPKQMRSIEDGNQTETADSGWHRVNKSQENDAY 721
            KLTEENIFLSNSLDIQKSKTEELCGEILPKQMRSIEDGNQTETADSGWHRVNKSQENDAY
Sbjct: 678  KLTEENIFLSNSLDIQKSKTEELCGEILPKQMRSIEDGNQTETADSGWHRVNKSQENDAY 737

Query: 722  QIKKQELFDDSFEFITLGRHLEEAYLVLQKLEKEIKGLQSNSASFSRSGSKVAAPAVSKL 781
            QIKKQELFDDSFEFITLGRHLEEAYLVLQKLEKEIKGLQSNSASFSRSGSKVAAPAVSKL
Sbjct: 738  QIKKQELFDDSFEFITLGRHLEEAYLVLQKLEKEIKGLQSNSASFSRSGSKVAAPAVSKL 797

Query: 782  IEAFETKVNEEHEVESEIQLPEDPYKLSSELVDNLGLLLHQVVVDSENASVLLKGERDHK 841
            IEAFETKVNEEHEVESEIQLPEDPYKLSSELVDNLGLLLHQVVVDSENASVLLKGERDHK
Sbjct: 798  IEAFETKVNEEHEVESEIQLPEDPYKLSSELVDNLGLLLHQVVVDSENASVLLKGERDHK 857

Query: 842  KVAISTLNKLTDQFEALENHSNDLVIANIDLGVLFEYLKHHVDDAGGKIYELENLNESLK 901
            KVAISTLNKLTDQFEALENHSNDLVIANIDLGVLFEYLKHHVDDAGGKIYELENLNESLK
Sbjct: 858  KVAISTLNKLTDQFEALENHSNDLVIANIDLGVLFEYLKHHVDDAGGKIYELENLNESLK 917

Query: 902  QQGLYHKISNCELAERLRGYDLKLTELQSQLCDLHQSSNEMVSSTCNQLDKLREGEIARA 961
            QQGLYHKISNCELAERLRGYDLKLTELQSQLCDLHQSSNEMVSSTCNQLDKLREGEIARA
Sbjct: 918  QQGLYHKISNCELAERLRGYDLKLTELQSQLCDLHQSSNEMVSSTCNQLDKLREGEIARA 977

Query: 962  TILEKDLQSFLLELAESIAKLDESLGKSDTAALKFCTNDQLPSCIIASVIDAVKMIDDLR 1021
            TILEKDLQSFLLELAESIAKLDESLGKSDTAALKFCTNDQLPSCIIASVIDAVKMIDDLR
Sbjct: 978  TILEKDLQSFLLELAESIAKLDESLGKSDTAALKFCTNDQLPSCIIASVIDAVKMIDDLR 1037

Query: 1022 ERLQATGADREAFRMLYEEVNEKYDNLFRRTEFSVDMLHRIYSELQKLYIASCGSVGGSD 1081
            ERLQATGADREAFRMLYEEVNEKYDNLFRRTEFSVDMLHRIYSELQKLYIASCGSVGGSD
Sbjct: 1038 ERLQATGADREAFRMLYEEVNEKYDNLFRRTEFSVDMLHRIYSELQKLYIASCGSVGGSD 1097

Query: 1082 MNMQIKGLGDPLDYSSFEAFIKLLEDCITERLQLESVNNKLRLDLEHMNVGFVDFSKRCL 1141
            MNMQIKGLGDPLDYSSFEAFIKLLEDCITERLQLESVNNKLRLDLEHMNVGFVDFSKRCL
Sbjct: 1098 MNMQIKGLGDPLDYSSFEAFIKLLEDCITERLQLESVNNKLRLDLEHMNVGFVDFSKRCL 1157

Query: 1142 DSPGIKKLITDVQSVLLLDDAEMDVEMPALYLESMVSLLLQKYKETELQLGLSREKSGSL 1201
            DSPGIKKLITDVQSVLLLDDAEMDVEMPALYLESMVSLLLQKYKETELQLGLSREKSGSL
Sbjct: 1158 DSPGIKKLITDVQSVLLLDDAEMDVEMPALYLESMVSLLLQKYKETELQLGLSREKSGSL 1217

Query: 1202 MMKLTELQESVHDLSTLILDHECEIVILKESLSQVQEALVASRSVLKDKANELEQSEQRV 1261
            MMKLTELQESVHDLSTLILDHECEIVILKESLSQVQEALVASRSVLKDKANELEQSEQRV
Sbjct: 1218 MMKLTELQESVHDLSTLILDHECEIVILKESLSQVQEALVASRSVLKDKANELEQSEQRV 1277

Query: 1262 SAIREKLSIAVAKGKGLIVQRDNLKQSLAQTSSELERCLQELQMKDTRLHETETKLTAYS 1321
            SAIREKLSIAVAKGKGLIVQRDNLKQSLAQTSSELERCLQELQMKDTRLHETETKLTAYS
Sbjct: 1278 SAIREKLSIAVAKGKGLIVQRDNLKQSLAQTSSELERCLQELQMKDTRLHETETKLTAYS 1337

Query: 1322 EAGERVEALESELSYIRNSATALRESFLLKDSVLQRIEEILDELDLPENFHSRDIIEKVD 1381
            EAGERVEALESELSYIRNSATALRESFLLKDSVLQRIEEILDELDLPENFHSRDIIEKVD
Sbjct: 1338 EAGERVEALESELSYIRNSATALRESFLLKDSVLQRIEEILDELDLPENFHSRDIIEKVD 1397

Query: 1382 WLAKSSTGENLPHTDWDQRSSVAGGSGSDANFVIMDAWKDEVQMDANVGDDLRRKYEELQ 1441
            WLAKSSTGENLPHTDWDQRSSVAGGSGSDANFVIMDAWKDEVQMDANVGDDLRRKYEELQ
Sbjct: 1398 WLAKSSTGENLPHTDWDQRSSVAGGSGSDANFVIMDAWKDEVQMDANVGDDLRRKYEELQ 1457

Query: 1442 TRFYGLAEQNEMLEQSLMERNNIVQRWEELLEKIDIPSQLRSMEPEDKMEWLHKSLSEAF 1501
            TRFYGLAEQNEMLEQSLMERNNIVQRWEELLEKIDIPSQLRSMEPEDKMEWLHKSLSEAF
Sbjct: 1458 TRFYGLAEQNEMLEQSLMERNNIVQRWEELLEKIDIPSQLRSMEPEDKMEWLHKSLSEAF 1517

Query: 1502 HDRDSLHQRVNYLENYCGLLTADLDDSQKKISDLEAELHSLVLEREKLSEKLEIIYHHNE 1561
            HDRDSLHQRVNYLENYCGLLTADLDDSQKKISDLEAELHSLVLEREKLSEKLEIIYHHNE
Sbjct: 1518 HDRDSLHQRVNYLENYCGLLTADLDDSQKKISDLEAELHSLVLEREKLSEKLEIIYHHNE 1577

Query: 1562 NLAFGNFENEVGIIVLQNELSNMQENLISTEHKIVKLEALVSNALQDEDVNDLVSGSSIE 1621
            NLAFGNFENEVGIIVLQNELSNMQENLISTEHKIVKLEALVSNALQDEDVNDLVSGSSIE
Sbjct: 1578 NLAFGNFENEVGIIVLQNELSNMQENLISTEHKIVKLEALVSNALQDEDVNDLVSGSSIE 1637

Query: 1622 FLDLMVTKLIQNYTASSSGKAVLGKATNGHDTEEEMLARSKNMHDAWQNDINILKKELED 1681
            FLDLMVTKLIQNYTASSSGKAVLGKATNGHDTEEEMLARSKNMHDAWQNDINILKKELED
Sbjct: 1638 FLDLMVTKLIQNYTASSSGKAVLGKATNGHDTEEEMLARSKNMHDAWQNDINILKKELED 1697

Query: 1682 ALNQLMVVTEERDQYMEMHESLVVKV---------------------------------- 1741
            ALNQLMVVTEERDQYMEMHESLVVKV                                  
Sbjct: 1698 ALNQLMVVTEERDQYMEMHESLVVKVGSSDKKKDELQELLNLEEQKSASMREKLEDAWHQ 1757

Query: 1742 ----------------------ESSDKKKDELQELLNLEEQKSASIREKLNVAVRKGKSL 1801
                                  ESSDKKKDELQELLNLEEQKSASIREKLNVAVRKGKSL
Sbjct: 1758 LMVVTEERDQYMEMHESLVVKVESSDKKKDELQELLNLEEQKSASIREKLNVAVRKGKSL 1817

Query: 1802 IQQRDSLKQAVEEMTTELKHLRSEMKSQENTLASYEQNFKDFSVYSGRAEALESENLSLK 1861
            IQQRDSLKQAVEEMTTELKHLRSEMKSQENTLASYEQNFKDFSVYSGRAEALESENLSLK
Sbjct: 1818 IQQRDSLKQAVEEMTTELKHLRSEMKSQENTLASYEQNFKDFSVYSGRAEALESENLSLK 1877

Query: 1862 NRLAEIERNFQEKEHKLSSIINTLVHIEDNVDVSENDPIEKLKQVGKLCSDLREAMYFSE 1921
            NRLAEIERNFQEKEHKLSSIINTLVHIEDNVDVSENDPIEKLKQVGKLCSDLREAMYFSE
Sbjct: 1878 NRLAEIERNFQEKEHKLSSIINTLVHIEDNVDVSENDPIEKLKQVGKLCSDLREAMYFSE 1937

Query: 1922 QESVKSRRAAELLLAELNEVQERNDTFQEELAKASDEIAELTKERDLAETSKLEALSELE 1981
            QESVKSRRAAELLLAELNEVQERNDTFQEELAKASDEIAELTKERDLAETSKLEALSELE
Sbjct: 1938 QESVKSRRAAELLLAELNEVQERNDTFQEELAKASDEIAELTKERDLAETSKLEALSELE 1997

Query: 1982 KLSTLHSKEKKIQYSQFMGLKSGLDRLKEAMREINCLLADAFSKDLDAFYNLEAAIQSCT 2041
            KLSTLHSKEKKIQYSQFMGLKSGLDRLKEAMREINCLLADAFSKDLDAFYNLEAAIQSCT
Sbjct: 1998 KLSTLHSKEKKIQYSQFMGLKSGLDRLKEAMREINCLLADAFSKDLDAFYNLEAAIQSCT 2057

Query: 2042 EPNGPADVNLSHSIVSGAFKSRDLKDKGKFFSLDSWSNFYTNAHVDENVATEIHSLVHQL 2101
            EPNGPADVNLSHSIVSGAFKSRDLKDKGKFFSLDSWSNFYTNAHVDENVATEIHSLVHQL
Sbjct: 2058 EPNGPADVNLSHSIVSGAFKSRDLKDKGKFFSLDSWSNFYTNAHVDENVATEIHSLVHQL 2117

Query: 2102 EESMKEIGALKEMIDGHSVSFHKQSDSLSKILGVLYREVNSQKELVQALKWDVQQSESVA 2161
            EESMKEIGALKEMIDGHSVSFHKQSDSLSKILGVLYREVNSQKELVQALKWDVQQSESVA
Sbjct: 2118 EESMKEIGALKEMIDGHSVSFHKQSDSLSKILGVLYREVNSQKELVQALKWDVQQSESVA 2177

Query: 2162 KDREMEGDILCRNIAMLFEACTSVIKEVDERKGELMGHDLTSGNLGMEIISTTPDQLSHA 2221
            KDREMEGDILCRNIAMLFEACTSVIKEVDERKGELMGHDLTSGNLGMEIISTTPDQLSHA
Sbjct: 2178 KDREMEGDILCRNIAMLFEACTSVIKEVDERKGELMGHDLTSGNLGMEIISTTPDQLSHA 2237

Query: 2222 GKTHLLSEESVRKIADRLLLAVREFIGFKAEMLDGSLKEMKVSVANLQKELQEKDIQKER 2281
            GKTHLLSEESVRKIADRLLLAVREFIGFKAEMLDGSLKEMKVSVANLQKELQEKDIQKER
Sbjct: 2238 GKTHLLSEESVRKIADRLLLAVREFIGFKAEMLDGSLKEMKVSVANLQKELQEKDIQKER 2297

Query: 2282 ICMELVGQIKEAEATASRYSIDLQASKDQVYELEKVTEQMDSERKVLEQRLREMQDGLSI 2341
            ICMELVGQIKEAEATASRYSIDLQASKDQVYELEKVTEQMDSERKVLEQRLREMQDGLSI
Sbjct: 2298 ICMELVGQIKEAEATASRYSIDLQASKDQVYELEKVTEQMDSERKVLEQRLREMQDGLSI 2357

Query: 2342 SDELRERVRLLTGSLAAKDQEIEALMHALDEEELQMEGLTKKIEDLERDLKQKNQELESI 2401
            SDELRERVRLLTGSLAAKDQEIEALMHALDEEELQMEGLTKKIEDLERDLKQKNQELESI
Sbjct: 2358 SDELRERVRLLTGSLAAKDQEIEALMHALDEEELQMEGLTKKIEDLERDLKQKNQELESI 2417

Query: 2402 EASRGKLMKKLSITVTKFDELHHLSESLLTEVEKLQAQLQDRDAEISFLRQEVTRCTNDA 2461
            EASRGKLMKKLSITVTKFDELHHLSESLLTEVEKLQAQLQDRDAEISFLRQEVTRCTNDA
Sbjct: 2418 EASRGKLMKKLSITVTKFDELHHLSESLLTEVEKLQAQLQDRDAEISFLRQEVTRCTNDA 2477

Query: 2462 LVATQTSNRNSEDINEVITWFDTMEARVGLSHSGHDDKENEVRECKEVLKKKITSILKGI 2521
            LVATQTSNRNSEDINEVITWFDTMEARVGLSHSGHDDKENEVRECKEVLKKKITSILKGI
Sbjct: 2478 LVATQTSNRNSEDINEVITWFDTMEARVGLSHSGHDDKENEVRECKEVLKKKITSILKGI 2537

Query: 2522 EDLQAESQRKDEMLLAEKNKVEELKRKELQLNLLEDVGDNNRASSVAPEIFESEPLHIVK 2581
            EDLQAESQRKDEMLLAEKNKVEELKRKELQLNLLEDVGDNNRASSVAPEIFESEPL    
Sbjct: 2538 EDLQAESQRKDEMLLAEKNKVEELKRKELQLNLLEDVGDNNRASSVAPEIFESEPL---- 2597

Query: 2582 SCILCTLQINKWAASSTSVTPQVRSLRKGNTTDQVAIAIDMDPASSSNRLEDEDDDKVHG 2641
                    INKWAASSTSVTPQVRSLRKGNTTDQVAIAIDMDPASSSNRLEDEDDDKVHG
Sbjct: 2598 --------INKWAASSTSVTPQVRSLRKGNTTDQVAIAIDMDPASSSNRLEDEDDDKVHG 2657

Query: 2642 FKSLASSRIVPKFSRRMTDMIDGLWVSCDRALMRQPALRLGIIIYWAILHALVATFVV 2643
            FKSLASSRIVPKFSRRMTDMIDGLWVSCDRALMRQPALRLGIIIYWAILHALVATFVV
Sbjct: 2658 FKSLASSRIVPKFSRRMTDMIDGLWVSCDRALMRQPALRLGIIIYWAILHALVATFVV 2703

BLAST of MC11g0525 vs. ExPASy TrEMBL
Match: A0A6J1IDN6 (golgin subfamily A member 4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111474373 PE=4 SV=1)

HSP 1 Score: 3796 bits (9843), Expect = 0.0
Identity = 2097/2667 (78.63%), Postives = 2307/2667 (86.50%), Query Frame = 0

Query: 2    LQQFRKKKDNKGTGSQGNSSKNKSKLEQQDADTEIVNVAAKPTSGSYSTDGVLASSFDCD 61
            LQQFRKKKDN+G GSQGNSSKN SKLEQ D D +IV  AAK  SGS STD  L+ S   +
Sbjct: 18   LQQFRKKKDNRGGGSQGNSSKNTSKLEQHDVDADIVTAAAKSPSGSCSTDEALSPSVYRN 77

Query: 62   ADNVDSSASPS-EHSSAAEIDHSTVYLKQEMDLAETSAIDEAEIPVEEVGYREDCDRLIQ 121
             D VDSSASPS EHS AAEIDHST  +KQEMDLAETSAID+AE+P++E+ YREDC+  IQ
Sbjct: 78   PDTVDSSASPSMEHSLAAEIDHSTDSVKQEMDLAETSAIDQAEVPMQEIAYREDCEHPIQ 137

Query: 122  NAEAAGVMSSGPSILIDAEVNNNHLCNLSSTESSSQISSASVDEQGRMVEVWSGCREEEI 181
            N EAAG M  G S+  DAE N+NH+CNLSSTESS QISS SV++QGR+ EVW GCREEE+
Sbjct: 138  NTEAAGFMPFGLSLPTDAEENDNHICNLSSTESSPQISSPSVEQQGRIAEVWGGCREEEL 197

Query: 182  SPARSASLLQAREDVGMTEDASMQSDQVCETQLAEDKQLQTGGMYESAAETTFKDTHCDE 241
             P++SASLLQAREDVGM EDA +QS QV ET+ + DK+L+TGGM ESAAETTFKD +CD+
Sbjct: 198  LPSQSASLLQAREDVGM-EDALVQSVQVHETEFSGDKKLETGGMNESAAETTFKDRYCDK 257

Query: 242  EEIITVDVASVSGFATVSNDFSISNPGENLGMQSSSSSSRDDWKEERQVHAEDMMHPSMY 301
            EEII VDV SVSG  T SN + IS+PGE LGM++SSSSSRDDWKEE Q HAEDM+  S  
Sbjct: 258  EEIIAVDVKSVSGADTESNSYLISSPGEKLGMKNSSSSSRDDWKEESQAHAEDMIQSSRC 317

Query: 302  QVQYMPEDNFAVQSEGHERPSQTSAKISEGGDADAIFPNAHMTTDLAVQSGTFYSFGQES 361
            +VQYMPEDNFA QSEGH+  SQTS    + GDA+AI  NAHMT+ L   SGTF SFGQ+S
Sbjct: 318  EVQYMPEDNFADQSEGHDMASQTS----DAGDANAISHNAHMTSTLDA-SGTFSSFGQDS 377

Query: 362  EFLDLLERVKEELIVTSFSKDISNLQISEQNVLQMELDNQHRKLTNDMSLVNTSLNEILE 421
            +FLDLLER+KEELIVTSFSKDI NLQI EQN LQ+ELDN   K TNDM+ +NTSLNE+LE
Sbjct: 378  KFLDLLERMKEELIVTSFSKDIFNLQIYEQNELQLELDNHLHKSTNDMTRLNTSLNEVLE 437

Query: 422  RNQSLVDELSQCRSELRDVSSAREELKNQLLTAEAEIENLSSRATESEKNLEKFHADMFR 481
            RNQSLV+ELS CRSEL+DVS+ +EEL+N LL AEAEIE LS+R +E+E +LEKFH DMFR
Sbjct: 438  RNQSLVNELSHCRSELKDVSTTKEELRNHLLNAEAEIEKLSTRTSETENSLEKFHGDMFR 497

Query: 482  LSKELDDCKHLVTALEEENERLNGVITSENENKMKLAEEKELYINENEKISSELSSFKSL 541
            L  ELDDCKHLVT LEEENERLNG+ITSENENK KLAEEKELYINENEKI  E+SSFKSL
Sbjct: 498  LGNELDDCKHLVTVLEEENERLNGIITSENENKRKLAEEKELYINENEKILLEISSFKSL 557

Query: 542  KVALEDENSKLIGSLSSVAEGKTKLEEEREQLFQVNGTLSVELENCRNLIATQQEEITNL 601
            K+ALE ENSKL+GS SSV E KTKLEEERE L QVNGTLSVEL NC+NL+ATQQEEIT+L
Sbjct: 558  KMALEVENSKLMGSFSSVVEEKTKLEEEREHLCQVNGTLSVELSNCKNLVATQQEEITDL 617

Query: 602  IKNLALVTEDRTKLEEDKTLLFHENEKMASELLVLDERLSAEYGERVRFEDDLRDALVRL 661
            IKNLAL TEDRTKLEEDK  LFHENE++ASELLVLDERLS E+ ERV+ E DL+DAL +L
Sbjct: 618  IKNLALATEDRTKLEEDKNRLFHENERIASELLVLDERLSTEHVERVKLESDLKDALAQL 677

Query: 662  EKLTEENIFLSNSLDIQKSKTEELCGEILPKQMRSIEDGNQTETADSGWHRVNKSQENDA 721
            ++LTEENIFLSN+LDI   K EELCGEIL  Q RS++D +Q E  DSG    NKSQ ND+
Sbjct: 678  DQLTEENIFLSNNLDIHIFKIEELCGEILSLQTRSVDDEDQAENTDSGRRHGNKSQGNDS 737

Query: 722  YQIK---------------------KQELFDDSFEFITLGRHLEEAYLVLQKLEKEIKGL 781
             QI                      +QE+FDDS E +TLG+HLEEA L+LQKLE+EI GL
Sbjct: 738  SQITFKENLHETSSVLAGGKPVIVTEQEIFDDSLELVTLGQHLEEADLMLQKLEREITGL 797

Query: 782  QSNSASFSRSGSKVAAPAVSKLIEAFETKVN-EEHEVESEIQLPEDPYKLSSELVDNLGL 841
            QSNSASFS SGSK+AAPA+SKLI+AFE+KVN EE EV++E QL  DPYKLS+ELVDNL +
Sbjct: 798  QSNSASFSSSGSKMAAPAISKLIQAFESKVNVEEQEVDAETQLSNDPYKLSNELVDNLRV 857

Query: 842  LLHQVVVDSENASVLLKGERDHKKVAISTLNKLTDQFEALENHSNDLVIANIDLGVLFEY 901
            LL QVVVDSE ASVLLKGERDH+KVAISTLNK  DQFE LENHSNDLV+ANI+  +LFE 
Sbjct: 858  LLRQVVVDSEKASVLLKGERDHRKVAISTLNKFKDQFEDLENHSNDLVMANIEHSILFEC 917

Query: 902  LKHHVDDAGGKIYELENLNESLKQQGLYHKISNCELAERLRGYDLKLTELQSQLCDLHQS 961
            LKHHV  AG KIYELE L ESLKQQG++HK SNCELAERL GY LKLTEL+SQLCD HQ 
Sbjct: 918  LKHHVYGAGDKIYELEILKESLKQQGVHHKNSNCELAERLCGYKLKLTELESQLCDFHQG 977

Query: 962  SNEMVSSTCNQLDKLREGEIARATILEKDLQSFLLELAESIAKLDESLGKSDTAALKFCT 1021
            SNE VS  CNQLD L+EGEI R   LEKD  SFLLELAE+IAKLDESLG S+T+A+KFCT
Sbjct: 978  SNETVSLICNQLDNLQEGEIERGMTLEKDWHSFLLELAETIAKLDESLGNSNTSAIKFCT 1037

Query: 1022 NDQLPSCIIASVIDAVKMIDDLRERLQATGADREAFRMLYEEVNEKYDNLFRRTEFSVDM 1081
            NDQLPSCI  SV +AV MIDDLRERLQAT +  EAFRMLYEEVNEKYDNLFR TE SVDM
Sbjct: 1038 NDQLPSCIATSVKNAVNMIDDLRERLQATASHGEAFRMLYEEVNEKYDNLFRSTELSVDM 1097

Query: 1082 LHRIYSELQKLYIASCGSVGGSDMNMQIKGLGDPLDYSSFEAFIKLLEDCITERLQLESV 1141
            LHRIY +LQ LYIASCGSV GSDMNMQ+K LGDPLDYS+FEA IK LEDCITERL+LES+
Sbjct: 1098 LHRIYGKLQNLYIASCGSVSGSDMNMQMKMLGDPLDYSNFEALIKPLEDCITERLRLESL 1157

Query: 1142 NNKLRLDLEHMNVGFVDFSKRCLDSPGIKKLITDVQSVLLLDDAEMD-VEMPALYLESMV 1201
            N+KLRLDLEH  V FV F +RCLD  GI+KLI +VQSVLLL+D E D  EMPA +LE+MV
Sbjct: 1158 NDKLRLDLEHRTVEFVQFRERCLDPIGIQKLIKNVQSVLLLEDTEKDRAEMPAFHLETMV 1217

Query: 1202 SLLLQKYKETELQLGLSREKSGSLMMKLTELQESVHDLSTLILDHECEIVILKESLSQVQ 1261
            SL+LQKY+E+ELQLGLSRE+ GS+M+KLTELQESVHDLSTLILDHECEIV+LKESLSQ Q
Sbjct: 1218 SLVLQKYRESELQLGLSREECGSVMIKLTELQESVHDLSTLILDHECEIVLLKESLSQAQ 1277

Query: 1262 EALVASRSVLKDKANELEQSEQRVSAIREKLSIAVAKGKGLIVQRDNLKQSLAQTSSELE 1321
            EAL+A RS LKDK +ELEQSEQRVSAIR+KLSIAVAKGKGLIVQRDNLKQ LAQTSSELE
Sbjct: 1278 EALMALRSELKDKVDELEQSEQRVSAIRDKLSIAVAKGKGLIVQRDNLKQLLAQTSSELE 1337

Query: 1322 RCLQELQMKDTRLHETETKLTAYSEAGERVEALESELSYIRNSATALRESFLLKDSVLQR 1381
            RCLQELQMKDTRLHE ETKL  YSEAGERVEALESELSYIRNSATALRESFLLKDSVLQR
Sbjct: 1338 RCLQELQMKDTRLHEVETKLNTYSEAGERVEALESELSYIRNSATALRESFLLKDSVLQR 1397

Query: 1382 IEEILDELDLPENFHSRDIIEKVDWLAKSSTGENLPHTDWDQRSSVAGGSGSDANFVIMD 1441
            IEEILDELDLPENFHSRDIIEK+DWLAKSS GEN+PHTDWDQRSSVAGGSGSDANFVI D
Sbjct: 1398 IEEILDELDLPENFHSRDIIEKIDWLAKSSAGENIPHTDWDQRSSVAGGSGSDANFVITD 1457

Query: 1442 AWKDEVQMDANVGDDLRRKYEELQTRFYGLAEQNEMLEQSLMERNNIVQRWEELLEKIDI 1501
            AWKDEVQ DANVGDDLRRKYEELQ++FYGLAEQNEMLEQSLMERNN VQRWEELLEKIDI
Sbjct: 1458 AWKDEVQPDANVGDDLRRKYEELQSKFYGLAEQNEMLEQSLMERNNAVQRWEELLEKIDI 1517

Query: 1502 PSQLRSMEPEDKMEWLHKSLSEAFHDRDSLHQRVNYLENYCGLLTADLDDSQKKISDLEA 1561
             S LRSMEPEDK+EWLH+SLSEA HDRDSLHQRVNYLENYCG LTADLDDS+KKISD+E+
Sbjct: 1518 HSHLRSMEPEDKIEWLHRSLSEACHDRDSLHQRVNYLENYCGSLTADLDDSRKKISDIES 1577

Query: 1562 ELHSLVLEREKLSEKLEIIYHHNENLAFGNFENEVGIIVLQNELSNMQENLISTEHKIVK 1621
            EL  ++LEREKLSEKLEII HHN++L+FG+FENE+  IVLQNELSNMQE LISTE KI+K
Sbjct: 1578 ELQLVLLEREKLSEKLEIIDHHNDHLSFGSFENEIENIVLQNELSNMQEKLISTERKILK 1637

Query: 1622 LEALVSNALQDEDVNDLVSGSSIEFLDLMVTKLIQNYTASSSGKAVLGKATNGHDTEEEM 1681
            LEALV N LQD DV+DLVSG+SIEF +LMV KL+QNYT  S   AV    TNG +TEE M
Sbjct: 1638 LEALVGNVLQDNDVHDLVSGNSIEFFELMVMKLVQNYTTFSLRDAVPESTTNGSNTEE-M 1697

Query: 1682 LARSKNMHDAWQNDINILKKELEDALNQLMVVTEERDQYMEMHESLVVKVESSDKKKDEL 1741
            LARS + H AWQNDIN+LKK+LEDA++QLMVVT+ERD+YMEMHE LVVKVES DKKKDEL
Sbjct: 1698 LARSVDAHVAWQNDINVLKKDLEDAMHQLMVVTKERDRYMEMHEYLVVKVESIDKKKDEL 1757

Query: 1742 QELLNLEEQKSASIREKLNVAVRKGKSLIQQRDSLKQAVEEMTTELKHLRSEMKSQENTL 1801
            QELLNLEEQKS SIREKLNVAVRKGKSL+QQRDSLKQA+EEMTTELK+LRSEMKSQEN L
Sbjct: 1758 QELLNLEEQKSTSIREKLNVAVRKGKSLVQQRDSLKQAIEEMTTELKNLRSEMKSQENIL 1817

Query: 1802 ASYEQNFKDFSVYSGRAEALESENLSLKNRLAEIERNFQEKEHKLSSIINTLVHIEDNVD 1861
            ASYEQ  KDFSVY+GR EALESENLSLKN+L E + N QEKE KLSSIINTLVH+E NVD
Sbjct: 1818 ASYEQKLKDFSVYTGRVEALESENLSLKNQLTETKNNLQEKEFKLSSIINTLVHMEVNVD 1877

Query: 1862 VSENDPIEKLKQVGKLCSDLREAMYFSEQESVKSRRAAELLLAELNEVQERNDTFQEELA 1921
            V E DPIEKLKQ+GKLCSDLREAM  SEQES KSRRAAELLLAELNEVQERND FQEELA
Sbjct: 1878 VYETDPIEKLKQLGKLCSDLREAMVSSEQESAKSRRAAELLLAELNEVQERNDAFQEELA 1937

Query: 1922 KASDEIAELTKERDLAETSKLEALSELEKLSTLHSKEKKIQYSQFMGLKSGLDRLKEAMR 1981
            KASDEIAELTKERDLAETSKLEALSELEKLSTLH KE+K Q+S+FMG KSGLD+LKEA+R
Sbjct: 1938 KASDEIAELTKERDLAETSKLEALSELEKLSTLHLKERKNQFSKFMGFKSGLDQLKEALR 1997

Query: 1982 EINCLLADAFSKDLDAFYNLEAAIQSCTEPNGPADVNLSHSIVSGAFKSRDLKDKGKFFS 2041
            EINCLLADAFS+DLDAFYNLE AI+SCT+ N  A+VN S S VSG  K    KDKG FF+
Sbjct: 1998 EINCLLADAFSRDLDAFYNLEVAIESCTKANDLAEVNPSPSTVSGVVK----KDKGSFFA 2057

Query: 2042 LDSWSNFYTNAHVDENVATEIHSLVHQ-LEESMKEIGALKEMIDGHSVSFHKQSDSLSKI 2101
            LD+  N Y N+ VDENV TEIHS + Q LEES+KEIGALKEMI GHSVSFHK+SDSLSK+
Sbjct: 2058 LDALLNSYANSPVDENVETEIHSQIMQHLEESIKEIGALKEMIGGHSVSFHKRSDSLSKV 2117

Query: 2102 LGVLYREVNSQKELVQALKWDVQQSESVAKDREMEGDILCRNIAMLFEACTSVIKEVDER 2161
            LG LY+EV SQKELVQAL+ DVQQ ESVAKD+E EGDILCRNIA+L EACTS IKE+++R
Sbjct: 2118 LGSLYQEVLSQKELVQALELDVQQRESVAKDKEKEGDILCRNIAVLSEACTSTIKEINQR 2177

Query: 2162 KGELMGHDLTSGNLGMEIISTTPDQLSHAGKTHLLSEESVRKIADRLLLAVREFIGFKAE 2221
            KGELMG+DLTS NLGM+I S TPDQLSH GKTHLLSEE V +IADRLL+ VREFIG KAE
Sbjct: 2178 KGELMGNDLTSENLGMDINSPTPDQLSHIGKTHLLSEEYVGRIADRLLITVREFIGLKAE 2237

Query: 2222 MLDGSLKEMKVSVANLQKELQEKDIQKERICMELVGQIKEAEATASRYSIDLQASKDQVY 2281
            M DG +KEMKV++ANLQKELQEKDIQ ER+CMELVGQIKEAEATA+RYS+DLQASKD++ 
Sbjct: 2238 MFDGHVKEMKVAIANLQKELQEKDIQNERVCMELVGQIKEAEATATRYSLDLQASKDEMR 2297

Query: 2282 ELEKVTEQMDSERKVLEQRLREMQDGLSISDELRERVRLLTGSLAAKDQEIEALMHALDE 2341
            EL+KVTEQM+SERK+LEQRLREM+DGLSISDELRE VR LT SLAAKDQEIEALMHALDE
Sbjct: 2298 ELQKVTEQMESERKILEQRLREMRDGLSISDELRETVRSLTDSLAAKDQEIEALMHALDE 2357

Query: 2342 EELQMEGLTKKIEDLERDLKQKNQELESIEASRGKLMKKLSITVTKFDELHHLSESLLTE 2401
            EE QMEGLT KIE+ E+ LKQKNQELESIE SRGKL KKLS+TVTKFDELHHLSESLLTE
Sbjct: 2358 EEEQMEGLTNKIEEKEKVLKQKNQELESIETSRGKLTKKLSLTVTKFDELHHLSESLLTE 2417

Query: 2402 VEKLQAQLQDRDAEISFLRQEVTRCTNDALVATQTSNRNSEDINEVITWFDTMEARVGLS 2461
            VEKLQAQLQDRDAE+SFLRQEVTRCTNDALVATQTSNR++EDINEVITW D MEARVGLS
Sbjct: 2418 VEKLQAQLQDRDAEVSFLRQEVTRCTNDALVATQTSNRSTEDINEVITWVDMMEARVGLS 2477

Query: 2462 HSGHDDKENEVRECKEVLKKKITSILKGIEDLQAESQRKDEMLLAEKNKVEELKRKELQL 2521
            H GHDD+EN++RECKEVLKKKITSILK IEDLQA SQRKD +LLAEKNKVEELKRKELQL
Sbjct: 2478 HIGHDDQENDIRECKEVLKKKITSILKEIEDLQAVSQRKDALLLAEKNKVEELKRKELQL 2537

Query: 2522 NLLEDVGDNNRASSVAPEIFESEPLHIVKSCILCTLQINKWAASSTSVTPQVRSLRKGNT 2581
            NLLEDVGD NRASS APEIFESEPL            INKWAASSTSVTPQV SLRKGNT
Sbjct: 2538 NLLEDVGDGNRASSAAPEIFESEPL------------INKWAASSTSVTPQVPSLRKGNT 2597

Query: 2582 TDQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRIVPKFSRRMTDMIDGLWVSCDRA 2641
             DQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRIVPKFSRR TDMIDGLWVSCDRA
Sbjct: 2598 -DQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRIVPKFSRRATDMIDGLWVSCDRA 2657

Query: 2642 LMRQPALRLGIIIYWAILHALVATFVV 2643
            LMRQPALRLGII YWAILHAL+ATFVV
Sbjct: 2658 LMRQPALRLGIIFYWAILHALLATFVV 2660

BLAST of MC11g0525 vs. ExPASy TrEMBL
Match: A0A6J1F6C6 (major antigen-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442749 PE=4 SV=1)

HSP 1 Score: 3794 bits (9840), Expect = 0.0
Identity = 2106/2667 (78.97%), Postives = 2308/2667 (86.54%), Query Frame = 0

Query: 2    LQQFRKKKDNKGTGSQGNSSKNKSKLEQQDADTEIVNVAAKPTSGSYSTDGVLASSFDCD 61
            LQQFRKKKDN+G GSQGNSSKN SKLEQ D D +IV  +AK  SGS STD  L+ S   D
Sbjct: 18   LQQFRKKKDNRGGGSQGNSSKNTSKLEQHDVDADIVTASAKSPSGSCSTDEALSPSVYRD 77

Query: 62   ADNVDSSASPS-EHSSAAEIDHSTVYLKQEMDLAETSAIDEAEIPVEEVGYREDCDRLIQ 121
             D VDSSASPS EHS AAEIDHST  +KQEMDLAETSAID+AE+P++EVGY EDC+  IQ
Sbjct: 78   PDAVDSSASPSMEHSLAAEIDHSTDSVKQEMDLAETSAIDQAEVPMQEVGYSEDCEHPIQ 137

Query: 122  NAEAAGVMSSGPSILIDAEVNNNHLCNLSSTESSSQISSASVDEQGRMVEVWSGCREEEI 181
            N EAA  M  G S+  DAE N+NH+CNLSSTESS QISSASV++QGR+ EVW GCREEE+
Sbjct: 138  NTEAA--MPFGLSLPTDAEENDNHICNLSSTESSPQISSASVEQQGRIAEVWGGCREEEL 197

Query: 182  SPARSASLLQAREDVGMTEDASMQSDQVCETQLAEDKQLQTGGMYESAAETTFKDTHCDE 241
             P++SASLLQAREDVGM ED  MQS Q  ET+ + DKQL+TGGM ESAAETTFKD +CD+
Sbjct: 198  LPSQSASLLQAREDVGM-EDVLMQSVQAHETEFSGDKQLETGGMNESAAETTFKDRYCDK 257

Query: 242  EEIITVDVASVSGFATVSNDFSISNPGENLGMQSSSSSSRDDWKEERQVHAEDMMHPSMY 301
            +EII  DV SVSG  T SN + IS+PGE LGM++SSSSSRDDWKEE QVHAEDM+  S  
Sbjct: 258  KEIIAADVKSVSGADTESNSYLISSPGEKLGMKNSSSSSRDDWKEESQVHAEDMIQSSRC 317

Query: 302  QVQYMPEDNFAVQSEGHERPSQTSAKISEGGDADAIFPNAHMTTDLAVQSGTFYSFGQES 361
            +VQYMPEDNFA QSEGH+  SQTS    + GDA+AI  NAHMT+  +  SGTF SF Q+S
Sbjct: 318  EVQYMPEDNFADQSEGHDMASQTS----DAGDANAISHNAHMTST-SDASGTFSSFEQDS 377

Query: 362  EFLDLLERVKEELIVTSFSKDISNLQISEQNVLQMELDNQHRKLTNDMSLVNTSLNEILE 421
            +FL LLER+KEELIVTSFSKDI NLQISEQN LQ+ELDN   K T+DM+ +NTSL+E+LE
Sbjct: 378  KFLHLLERMKEELIVTSFSKDIFNLQISEQNELQLELDNHLHKSTDDMTRLNTSLDEVLE 437

Query: 422  RNQSLVDELSQCRSELRDVSSAREELKNQLLTAEAEIENLSSRATESEKNLEKFHADMFR 481
            RNQSLVDELS CRSEL+DV + +EEL++QLL AEAEIE LSSR +E+E +LEKFH DMFR
Sbjct: 438  RNQSLVDELSHCRSELKDVLTTKEELRDQLLNAEAEIEKLSSRTSETENSLEKFHGDMFR 497

Query: 482  LSKELDDCKHLVTALEEENERLNGVITSENENKMKLAEEKELYINENEKISSELSSFKSL 541
            L KELDDCKHLVT LEEENERLNG+ITSENENK KLAEEKELYINENEKI SE+SSFKSL
Sbjct: 498  LGKELDDCKHLVTVLEEENERLNGIITSENENKRKLAEEKELYINENEKILSEISSFKSL 557

Query: 542  KVALEDENSKLIGSLSSVAEGKTKLEEEREQLFQVNGTLSVELENCRNLIATQQEEITNL 601
            K+ALE ENSKL+GSLS V E KTKLEEERE L Q+NGTLSVEL NC+NL+ATQQEEIT+L
Sbjct: 558  KMALEVENSKLMGSLSEVVEEKTKLEEEREHLCQMNGTLSVELSNCKNLVATQQEEITDL 617

Query: 602  IKNLALVTEDRTKLEEDKTLLFHENEKMASELLVLDERLSAEYGERVRFEDDLRDALVRL 661
            IKNLAL TEDRTKLEEDK  LFHENE++ASELLVLDERLS E+ ERV+ E DL+DAL +L
Sbjct: 618  IKNLALATEDRTKLEEDKNRLFHENERIASELLVLDERLSTEHEERVKLESDLKDALAQL 677

Query: 662  EKLTEENIFLSNSLDIQKSKTEELCGEILPKQMRSIEDGNQTETADSGWHRVNKSQENDA 721
            ++LTEENIFLSN+LDI   K EELCGEIL  Q RS++D +Q E  DSG    NK Q ND+
Sbjct: 678  DQLTEENIFLSNNLDIHIFKIEELCGEILSLQTRSVDDEDQAENTDSGRRHGNKFQGNDS 737

Query: 722  YQIK---------------------KQELFDDSFEFITLGRHLEEAYLVLQKLEKEIKGL 781
             QI                      +QE+FDDS   +TLG+HLEEA L+LQKLEKEIKGL
Sbjct: 738  SQITFKENLHEISSVLAGGKPFIVTEQEIFDDSLGLVTLGQHLEEADLMLQKLEKEIKGL 797

Query: 782  QSNSASFSRSGSKVAAPAVSKLIEAFETKVN-EEHEVESEIQLPEDPYKLSSELVDNLGL 841
            QSNSASFS SGSK+AAPAVSKLI+AFE+KVN EE EV++EIQL  DPYKLS+ELVDNL +
Sbjct: 798  QSNSASFSSSGSKMAAPAVSKLIQAFESKVNVEEQEVDAEIQLSNDPYKLSNELVDNLRV 857

Query: 842  LLHQVVVDSENASVLLKGERDHKKVAISTLNKLTDQFEALENHSNDLVIANIDLGVLFEY 901
            LL QVVVDSE ASVLLKGERDH+KVAISTLN+  DQFE LENHSNDLV+ANI+  +LFE 
Sbjct: 858  LLRQVVVDSEKASVLLKGERDHRKVAISTLNEFKDQFEDLENHSNDLVMANIEHSILFEC 917

Query: 902  LKHHVDDAGGKIYELENLNESLKQQGLYHKISNCELAERLRGYDLKLTELQSQLCDLHQS 961
            LKHHV DAG KIYELE L ESLKQQG++HK SNCELA RL GY LKLTEL+SQLCD HQ 
Sbjct: 918  LKHHVYDAGDKIYELEILKESLKQQGVHHKNSNCELAVRLCGYKLKLTELESQLCDFHQG 977

Query: 962  SNEMVSSTCNQLDKLREGEIARATILEKDLQSFLLELAESIAKLDESLGKSDTAALKFCT 1021
            SNE VS  CNQLD L+EGEI R   LEKD  SFLLELAE+IAKLDESLG S+T+A+KFCT
Sbjct: 978  SNETVSLICNQLDNLQEGEIERGMTLEKDWHSFLLELAETIAKLDESLGNSNTSAIKFCT 1037

Query: 1022 NDQLPSCIIASVIDAVKMIDDLRERLQATGADREAFRMLYEEVNEKYDNLFRRTEFSVDM 1081
            NDQLPSCI  SV +AV +IDDLRERLQAT ++ EAFRMLYEEVNEKYDNLFR TE SVDM
Sbjct: 1038 NDQLPSCIATSVKNAVNIIDDLRERLQATASNGEAFRMLYEEVNEKYDNLFRSTELSVDM 1097

Query: 1082 LHRIYSELQKLYIASCGSVGGSDMNMQIKGLGDPLDYSSFEAFIKLLEDCITERLQLESV 1141
            L RIY +LQ LYIASCGSV GSDMNMQIK LGDPLDYS+FE  IK LEDCITERL+LES+
Sbjct: 1098 LRRIYGKLQNLYIASCGSVSGSDMNMQIKMLGDPLDYSNFETLIKPLEDCITERLRLESL 1157

Query: 1142 NNKLRLDLEHMNVGFVDFSKRCLDSPGIKKLITDVQSVLLLDDAEMD-VEMPALYLESMV 1201
            N+KLRLDLEH  V FV F +RCLD  GI+KLI +VQSVLLL+D E D  EMPA +LE+MV
Sbjct: 1158 NDKLRLDLEHRTVEFVQFRERCLDPIGIQKLIKNVQSVLLLEDTEKDRAEMPAFHLETMV 1217

Query: 1202 SLLLQKYKETELQLGLSREKSGSLMMKLTELQESVHDLSTLILDHECEIVILKESLSQVQ 1261
            SL+LQKY+E+ELQLGLSRE+ GS+MMKLTELQESVHDLSTLILDHECEIV+LKESLSQ Q
Sbjct: 1218 SLVLQKYRESELQLGLSREECGSVMMKLTELQESVHDLSTLILDHECEIVLLKESLSQAQ 1277

Query: 1262 EALVASRSVLKDKANELEQSEQRVSAIREKLSIAVAKGKGLIVQRDNLKQSLAQTSSELE 1321
            EAL+A RS LKDK +ELEQSEQRVSAIR+KLSIAVAKGKGLIVQRDNLKQ LAQTSSELE
Sbjct: 1278 EALMALRSELKDKVDELEQSEQRVSAIRDKLSIAVAKGKGLIVQRDNLKQLLAQTSSELE 1337

Query: 1322 RCLQELQMKDTRLHETETKLTAYSEAGERVEALESELSYIRNSATALRESFLLKDSVLQR 1381
            RCLQELQMKDTRLHE ETKL  YSEAGERVEALESELSYIRNSATALRESFLLKDSVLQR
Sbjct: 1338 RCLQELQMKDTRLHEVETKLNTYSEAGERVEALESELSYIRNSATALRESFLLKDSVLQR 1397

Query: 1382 IEEILDELDLPENFHSRDIIEKVDWLAKSSTGENLPHTDWDQRSSVAGGSGSDANFVIMD 1441
            IEEILDELDLPENFHSRDIIEK+DWLAKSS GEN+PHTDWDQRSSVAGGSGSDANFVI D
Sbjct: 1398 IEEILDELDLPENFHSRDIIEKIDWLAKSSAGENIPHTDWDQRSSVAGGSGSDANFVITD 1457

Query: 1442 AWKDEVQMDANVGDDLRRKYEELQTRFYGLAEQNEMLEQSLMERNNIVQRWEELLEKIDI 1501
            AWKDEVQ DANVGDDLRRKYEELQT+FYGLAEQNEMLEQSLMERNN VQRWEELLEKIDI
Sbjct: 1458 AWKDEVQPDANVGDDLRRKYEELQTKFYGLAEQNEMLEQSLMERNNAVQRWEELLEKIDI 1517

Query: 1502 PSQLRSMEPEDKMEWLHKSLSEAFHDRDSLHQRVNYLENYCGLLTADLDDSQKKISDLEA 1561
             S LRSMEPEDK+EWL++SLSEA HDRDSLHQRVNYLENYCG LTADLDDS+KKISD+EA
Sbjct: 1518 HSHLRSMEPEDKIEWLNRSLSEACHDRDSLHQRVNYLENYCGSLTADLDDSRKKISDIEA 1577

Query: 1562 ELHSLVLEREKLSEKLEIIYHHNENLAFGNFENEVGIIVLQNELSNMQENLISTEHKIVK 1621
            EL  ++LEREKLSEKLEII HHN++L+FG FENE+  IVLQNELSNMQE LISTE KIVK
Sbjct: 1578 ELQLVLLEREKLSEKLEIIDHHNDHLSFGTFENEIENIVLQNELSNMQEKLISTELKIVK 1637

Query: 1622 LEALVSNALQDEDVNDLVSGSSIEFLDLMVTKLIQNYTASSSGKAVLGKATNGHDTEEEM 1681
            LEALV N LQD DV+DLVSGSSIEFL+LMV KL+QNYT S    AV    TNG  T EEM
Sbjct: 1638 LEALVGNVLQDNDVHDLVSGSSIEFLELMVMKLVQNYTFSLRD-AVPESTTNG-STTEEM 1697

Query: 1682 LARSKNMHDAWQNDINILKKELEDALNQLMVVTEERDQYMEMHESLVVKVESSDKKKDEL 1741
            LARS + H AWQNDIN+LKK+LEDA++QLMVVT+ERD+YMEMHE LVVKVES DKKKDEL
Sbjct: 1698 LARSVDAHVAWQNDINVLKKDLEDAMHQLMVVTKERDRYMEMHEYLVVKVESIDKKKDEL 1757

Query: 1742 QELLNLEEQKSASIREKLNVAVRKGKSLIQQRDSLKQAVEEMTTELKHLRSEMKSQENTL 1801
            QELLNLEEQKS SIREKLNVAVRKGKSL+QQRDSLKQA+EEMTTELK+LRSEMKSQENTL
Sbjct: 1758 QELLNLEEQKSTSIREKLNVAVRKGKSLVQQRDSLKQAIEEMTTELKNLRSEMKSQENTL 1817

Query: 1802 ASYEQNFKDFSVYSGRAEALESENLSLKNRLAEIERNFQEKEHKLSSIINTLVHIEDNVD 1861
            ASYEQ  +DFSVY+GR EALESENLSLKN+L E + N QEKE KLSSIINTLVH+E NVD
Sbjct: 1818 ASYEQKLRDFSVYTGRVEALESENLSLKNQLTETKNNLQEKEFKLSSIINTLVHMEVNVD 1877

Query: 1862 VSENDPIEKLKQVGKLCSDLREAMYFSEQESVKSRRAAELLLAELNEVQERNDTFQEELA 1921
            V E DPIEKLKQVGKLCSDLREAM  SEQESVKSRRAAELLLAELNEVQERND FQEELA
Sbjct: 1878 VYETDPIEKLKQVGKLCSDLREAMVSSEQESVKSRRAAELLLAELNEVQERNDAFQEELA 1937

Query: 1922 KASDEIAELTKERDLAETSKLEALSELEKLSTLHSKEKKIQYSQFMGLKSGLDRLKEAMR 1981
            KASDEIAELTKERDLAETSKLEALSELEKLSTLH KE+K Q+S+FMG KSGLD+LKEA+R
Sbjct: 1938 KASDEIAELTKERDLAETSKLEALSELEKLSTLHLKERKNQFSKFMGFKSGLDQLKEALR 1997

Query: 1982 EINCLLADAFSKDLDAFYNLEAAIQSCTEPNGPADVNLSHSIVSGAFKSRDLKDKGKFFS 2041
            EINCLLADAFS+DLDAFYNLE AI+SCT+ N  A+VN S S VSG  K    KDKG FF+
Sbjct: 1998 EINCLLADAFSRDLDAFYNLEVAIESCTKANDLAEVNPSPSTVSGVVK----KDKGSFFA 2057

Query: 2042 LDSWSNFYTNAHVDENVATEIHSLVHQ-LEESMKEIGALKEMIDGHSVSFHKQSDSLSKI 2101
            LD+W N Y N+ VDENV TEIHS + Q LEES+KEIGALKEMI GHSVSFHK+SDSLSK+
Sbjct: 2058 LDTWLNSYANSPVDENVETEIHSQIMQHLEESIKEIGALKEMIGGHSVSFHKRSDSLSKV 2117

Query: 2102 LGVLYREVNSQKELVQALKWDVQQSESVAKDREMEGDILCRNIAMLFEACTSVIKEVDER 2161
            LG LY+EV SQKELVQAL+ DVQQ ESVAKD+E EGDILCRNIA+L EACTS IKE+D+R
Sbjct: 2118 LGSLYQEVLSQKELVQALELDVQQRESVAKDKEKEGDILCRNIAVLSEACTSTIKEIDQR 2177

Query: 2162 KGELMGHDLTSGNLGMEIISTTPDQLSHAGKTHLLSEESVRKIADRLLLAVREFIGFKAE 2221
            KGELMG+DLTS NLGM+I S TPDQLSH GKTHLLSEE VR+IADRLL+ VREFIG KAE
Sbjct: 2178 KGELMGNDLTSENLGMDINSPTPDQLSHIGKTHLLSEEYVRRIADRLLITVREFIGLKAE 2237

Query: 2222 MLDGSLKEMKVSVANLQKELQEKDIQKERICMELVGQIKEAEATASRYSIDLQASKDQVY 2281
            M DG +KEMK ++ANLQKELQEKDIQ ER+CMELVGQIKEAEATA+RYS+DLQASKD++ 
Sbjct: 2238 MFDGHVKEMKAAIANLQKELQEKDIQNERVCMELVGQIKEAEATATRYSLDLQASKDEMR 2297

Query: 2282 ELEKVTEQMDSERKVLEQRLREMQDGLSISDELRERVRLLTGSLAAKDQEIEALMHALDE 2341
            EL+KVTEQM+SERK+LEQRLREM+DGLSISDELRE VRLLT SLAAKDQEIEALMHALDE
Sbjct: 2298 ELQKVTEQMESERKILEQRLREMRDGLSISDELRETVRLLTDSLAAKDQEIEALMHALDE 2357

Query: 2342 EELQMEGLTKKIEDLERDLKQKNQELESIEASRGKLMKKLSITVTKFDELHHLSESLLTE 2401
            EE QMEGLT KIE+ E+ LKQKNQELESIE SRGKL KKLS+TVTKFDELHHLSESLLTE
Sbjct: 2358 EEEQMEGLTNKIEEQEKVLKQKNQELESIETSRGKLTKKLSLTVTKFDELHHLSESLLTE 2417

Query: 2402 VEKLQAQLQDRDAEISFLRQEVTRCTNDALVATQTSNRNSEDINEVITWFDTMEARVGLS 2461
            VEKLQAQLQDRDAE+SFLRQEVTRCTNDALVATQTSNR++EDINEVITWFD MEARVGLS
Sbjct: 2418 VEKLQAQLQDRDAEVSFLRQEVTRCTNDALVATQTSNRSTEDINEVITWFDMMEARVGLS 2477

Query: 2462 HSGHDDKENEVRECKEVLKKKITSILKGIEDLQAESQRKDEMLLAEKNKVEELKRKELQL 2521
            H GHDD+EN VRECKEVLKKKITSILK IEDLQA SQRKD +LLAEKNKVEELKRKELQL
Sbjct: 2478 HIGHDDQENGVRECKEVLKKKITSILKEIEDLQAVSQRKDALLLAEKNKVEELKRKELQL 2537

Query: 2522 NLLEDVGDNNRASSVAPEIFESEPLHIVKSCILCTLQINKWAASSTSVTPQVRSLRKGNT 2581
            NLLEDVGD NRASS APEIFESEPL            INKWAASSTSVTPQV SLRKGNT
Sbjct: 2538 NLLEDVGDGNRASSAAPEIFESEPL------------INKWAASSTSVTPQVPSLRKGNT 2597

Query: 2582 TDQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRIVPKFSRRMTDMIDGLWVSCDRA 2641
             DQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRIVPKFSRR TDMIDGLWVSCDRA
Sbjct: 2598 -DQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRIVPKFSRRATDMIDGLWVSCDRA 2657

Query: 2642 LMRQPALRLGIIIYWAILHALVATFVV 2643
            LMRQPALRLGII YWAILHAL+ATFVV
Sbjct: 2658 LMRQPALRLGIIFYWAILHALLATFVV 2657

BLAST of MC11g0525 vs. ExPASy TrEMBL
Match: A0A6J1J1D8 (centrosome-associated protein CEP250-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480399 PE=4 SV=1)

HSP 1 Score: 3754 bits (9734), Expect = 0.0
Identity = 2085/2667 (78.18%), Postives = 2298/2667 (86.16%), Query Frame = 0

Query: 2    LQQFRKKKDNKGTGSQGNSSKNKSKLEQQDADTEIVNVAAKPTSGSYSTDGVLASSFDCD 61
            LQQFRKKKD KG GSQG+SSK+ +KLEQ DAD +  +      SGS+STDGVLAS+ DC 
Sbjct: 18   LQQFRKKKDYKGRGSQGSSSKHTNKLEQHDADADTASTGVL-ASGSHSTDGVLASAVDCS 77

Query: 62   ADNVDSSASPS-EHSSAAEIDHSTVYLKQEMDLAETSAIDEAEIPVEEVGYREDCDRLIQ 121
             D V SSASPS E + AAE+DHST  +KQE+DLAETS ID+ E+P++EVGYRED D  IQ
Sbjct: 78   PDTVGSSASPSTELALAAEVDHSTDSVKQEIDLAETSEIDQEEVPMQEVGYREDYDHPIQ 137

Query: 122  NAEAAGVMSSGPSILIDAEVNNNHLCNLSSTESSSQISSASVDEQGRMVEVWSGCREEEI 181
            NAE+AGV SS PS+   AE NN+ + NLSS+ESSSQISSASV++Q ++VEVW GCR EE+
Sbjct: 138  NAESAGVRSSEPSLAPAAEENNDDIYNLSSSESSSQISSASVEQQQKIVEVWGGCRGEEL 197

Query: 182  SPARSASLLQAREDVGMTEDASMQSDQVCETQLAEDKQLQTGGMYESAAETTFKDTHCDE 241
                SASL QAREDVGM  D  MQS  +C T+LAEDKQ++TGGM ESAAETTFKDT CD 
Sbjct: 198  LLPSSASLSQAREDVGMKGDDLMQSGPLCGTELAEDKQVETGGMNESAAETTFKDTCCDG 257

Query: 242  EEIITVDVASVSGFATVSNDFSISNPGENLGMQSSSSSSRDDWKEERQVHAEDMMHPSMY 301
            ++IIT DVASVS   T SN +SIS+PGE LGMQ+SSSS R+DWKE RQVHAEDM+H S  
Sbjct: 258  DKIITADVASVSSAGTESNSYSISSPGEKLGMQNSSSSGRNDWKEVRQVHAEDMIHSSRS 317

Query: 302  QVQYMPEDNFAVQSEGHERPSQTSAKISEGGDADAIFPNAHMTTDLAVQSGTFYSFGQES 361
            QVQYMPEDNFA +SE H+ PSQTS KIS+GGD D +  NAHMTT  A  SGTF SFGQ S
Sbjct: 318  QVQYMPEDNFADKSESHKSPSQTSVKISDGGDVDTLSHNAHMTTTYA-HSGTFSSFGQNS 377

Query: 362  EFLDLLERVKEELIVTSFSKDISNLQISEQNVLQMELDNQHRKLTNDMSLVNTSLNEILE 421
            +FLDLLERVKEELIVTSFSKDI N QISEQN LQM+LD                  E+L 
Sbjct: 378  KFLDLLERVKEELIVTSFSKDIFNFQISEQNELQMKLD------------------EVLV 437

Query: 422  RNQSLVDELSQCRSELRDVSSAREELKNQLLTAEAEIENLSSRATESEKNLEKFHADMFR 481
            RN +LVDELS CRSEL+DVS A EEL+NQLL AEAEI+ LSSRA+E+E + EKFH DMFR
Sbjct: 438  RNHTLVDELSHCRSELKDVSVANEELRNQLLAAEAEIQKLSSRASETENSFEKFHGDMFR 497

Query: 482  LSKELDDCKHLVTALEEENERLNGVITSENENKMKLAEEKELYINENEKISSELSSFKSL 541
            L KELDDCKHLV+ LEEENERLNG+IT ENENK KLA+EKELYI ENEKI SELSSFKSL
Sbjct: 498  LEKELDDCKHLVSVLEEENERLNGIITFENENKKKLAKEKELYIGENEKILSELSSFKSL 557

Query: 542  KVALEDENSKLIGSLSSVAEGKTKLEEEREQLFQVNGTLSVELENCRNLIATQQEEITNL 601
            K ALE ENS+L+GSLSS+AE K K EEERE LFQVNGTLSVEL NC++L+ATQQEEITNL
Sbjct: 558  KAALEVENSELMGSLSSIAEEKIKHEEEREHLFQVNGTLSVELANCKSLVATQQEEITNL 617

Query: 602  IKNLALVTEDRTKLEEDKTLLFHENEKMASELLVLDERLSAEYGERVRFEDDLRDALVRL 661
            I NLAL+TED+ +LEEDK LL HENEKM SELLVLDERLS E+ ERVRFEDDL+DA+V++
Sbjct: 618  INNLALLTEDKVRLEEDKNLLLHENEKMRSELLVLDERLSTEHEERVRFEDDLKDAIVQV 677

Query: 662  EKLTEENIFLSNSLDIQKSKTEELCGEILPKQMRSIEDGNQTETADSGWHRVNKSQENDA 721
            ++LTE+N FLS+SLDI K K EELCGEIL  + RS ED +Q   ADSG H  NK QEND+
Sbjct: 678  KQLTEDNGFLSSSLDIHKFKVEELCGEILSLKTRSREDEDQAGNADSGLHHENKFQENDS 737

Query: 722  YQ--------------------IKKQELFDDSFEFITLGRHLEEAYLVLQKLEKEIKGLQ 781
            YQ                    + +QE FDDS  F+ LGRHLEEA ++LQKLEKEIKGLQ
Sbjct: 738  YQTTFKKNLHGTSVLAVGKPFIVTEQENFDDSLGFVILGRHLEEADVILQKLEKEIKGLQ 797

Query: 782  SNSASFSRSGSKVAAPAVSKLIEAFETKVN-EEHEVESEIQLPEDPYKLSSELVDNLGLL 841
            SNSASFSRSGSK+ APAVSKLI+AFE+KVN EE+EVE EIQLP DPYKLS+E VDNL  L
Sbjct: 798  SNSASFSRSGSKMDAPAVSKLIQAFESKVNVEENEVEDEIQLP-DPYKLSNEFVDNLRAL 857

Query: 842  LHQVVVDSENASVLLKGERDHKKVAISTLNKLTDQFEALENHSNDLVIANIDLGVLFEYL 901
            L QVV+D+ENASVLLKGERDH+KVAISTL++LTDQFEAL+NHSNDLVIANI+ GVLFE L
Sbjct: 858  LRQVVIDAENASVLLKGERDHRKVAISTLSELTDQFEALKNHSNDLVIANIEHGVLFECL 917

Query: 902  KHHVDDAGGKIYELENLNESLKQQGLYHKISNCELAERLRGYDLKLTELQSQLCDLHQSS 961
            KHHVDDA GKIYELE LNESL+QQG++HK SN ELAERL GY+LKLTEL+ QLCDLHQSS
Sbjct: 918  KHHVDDADGKIYELEILNESLRQQGVHHKSSNSELAERLCGYELKLTELECQLCDLHQSS 977

Query: 962  NEMVSSTCNQLDKLREGEIARATILEKDLQSFLLELAESIAKLDESLGKSDTAALKFCTN 1021
            N+MVS  CNQLD L++G I R  ILEKD  SFLLELAE+IAKLDES+GKSDT+A+KFCTN
Sbjct: 978  NQMVSLICNQLDNLQDGAIKREIILEKDRHSFLLELAETIAKLDESVGKSDTSAIKFCTN 1037

Query: 1022 DQLPSCIIASVIDAVKMIDDLRERLQATGADREAFRMLYEEVNEKYDNLFRRTEFSVDML 1081
            DQ PSC+ +SV DAVKMI DLRERLQAT AD EAFRM YEEVNEKYDNLFRRTE SVD+L
Sbjct: 1038 DQFPSCLASSVTDAVKMIHDLRERLQATAADGEAFRMSYEEVNEKYDNLFRRTECSVDLL 1097

Query: 1082 HRIYSELQKLYIASCGSVGGSDMNMQIKGLGDPLDYSSFEAFIKLLEDCITERLQLESVN 1141
            H+I+ ELQKLY+ASC SVGGSDMNMQIK LGDPLDYSSFEA IK LED IT+RLQLESVN
Sbjct: 1098 HKIFGELQKLYLASCESVGGSDMNMQIKMLGDPLDYSSFEAVIKPLEDFITQRLQLESVN 1157

Query: 1142 NKLRLDLEHMNVGFVDFSKRCLDSPGIKKLITDVQSVLLL-DDAEMD-VEMPALYLESMV 1201
            NKLRLDLEH  V  VDFSKRCLDS GI+KLI DVQSVLLL +D E D V+MPALYL+S++
Sbjct: 1158 NKLRLDLEHRTVELVDFSKRCLDSTGIEKLIKDVQSVLLLPEDTEGDCVQMPALYLQSII 1217

Query: 1202 SLLLQKYKETELQLGLSREKSGSLMMKLTELQESVHDLSTLILDHECEIVILKESLSQVQ 1261
            SLL+QKYKETELQLGLSRE+ GS MMKLTELQ SVHDLSTLILDHE EIVILKESLSQ Q
Sbjct: 1218 SLLIQKYKETELQLGLSREEYGSAMMKLTELQGSVHDLSTLILDHEGEIVILKESLSQAQ 1277

Query: 1262 EALVASRSVLKDKANELEQSEQRVSAIREKLSIAVAKGKGLIVQRDNLKQSLAQTSSELE 1321
            EAL+ASRS LKDK NELEQSEQRVSAIREKLSIAV KGKGLIVQRD+LKQSLAQTSSELE
Sbjct: 1278 EALMASRSELKDKLNELEQSEQRVSAIREKLSIAVTKGKGLIVQRDSLKQSLAQTSSELE 1337

Query: 1322 RCLQELQMKDTRLHETETKLTAYSEAGERVEALESELSYIRNSATALRESFLLKDSVLQR 1381
            RCLQELQMKD RL ETETKL  YSEAGERVEALESELSYIRNSATALRESFLLKDSVLQR
Sbjct: 1338 RCLQELQMKDNRLLETETKLKTYSEAGERVEALESELSYIRNSATALRESFLLKDSVLQR 1397

Query: 1382 IEEILDELDLPENFHSRDIIEKVDWLAKSSTGENLPHTDWDQRSSVAGGSGSDANFVIMD 1441
            I+EILD LDLPENFHSRDIIEKVDWLAKSSTG+NLP TD DQRSSV GGSGSDANFV  D
Sbjct: 1398 IDEILDALDLPENFHSRDIIEKVDWLAKSSTGKNLPQTDGDQRSSVTGGSGSDANFVTTD 1457

Query: 1442 AWKDEVQMDANVGDDLRRKYEELQTRFYGLAEQNEMLEQSLMERNNIVQRWEELLEKIDI 1501
             WKDE+Q DANVGDDLRRKYEELQT+FYGLAEQNEMLEQSLMERNN+VQRWEELLEKID 
Sbjct: 1458 GWKDEMQTDANVGDDLRRKYEELQTKFYGLAEQNEMLEQSLMERNNLVQRWEELLEKIDT 1517

Query: 1502 PSQLRSMEPEDKMEWLHKSLSEAFHDRDSLHQRVNYLENYCGLLTADLDDSQKKISDLEA 1561
            PS LRS+EPEDK+EWLH+SL+EA HDRDSLHQRVN LEN+CGLLTADLDDS+KKISD+EA
Sbjct: 1518 PSHLRSIEPEDKIEWLHRSLTEACHDRDSLHQRVNNLENHCGLLTADLDDSRKKISDIEA 1577

Query: 1562 ELHSLVLEREKLSEKLEIIYHHNENLAFGNFENEVGIIVLQNELSNMQENLISTEHKIVK 1621
            ELHS++LEREKLSEKLEI+Y HNE+L+FG FENEV II+LQNELSNMQ+ +ISTEHKIVK
Sbjct: 1578 ELHSVMLEREKLSEKLEIVYDHNEHLSFGTFENEVEIIILQNELSNMQDKIISTEHKIVK 1637

Query: 1622 LEALVSNALQDEDVNDLVSGSSIEFLDLMVTKLIQNYTASSSGKAVLGKATNGHDTEEEM 1681
            LEALVSNAL+D D+NDLVSGSSIEFL+LMV KL+QNYTASS G   LG+ATNG D EE +
Sbjct: 1638 LEALVSNALRDMDMNDLVSGSSIEFLELMVMKLVQNYTASSLGNVELGRATNGPDAEE-V 1697

Query: 1682 LARSKNMHDAWQNDINILKKELEDALNQLMVVTEERDQYMEMHESLVVKVESSDKKKDEL 1741
            +ARS +    WQN+IN  KKELE A++QLMVVT+ERDQYM MHESLVVKVES D+KKDEL
Sbjct: 1698 VARSIDTQVGWQNEINYHKKELEYAVHQLMVVTKERDQYMGMHESLVVKVESLDRKKDEL 1757

Query: 1742 QELLNLEEQKSASIREKLNVAVRKGKSLIQQRDSLKQAVEEMTTELKHLRSEMKSQENTL 1801
            QELL+LEEQK  SIREKLNVAVRKGKSL+QQRDSLKQA+EEMTTEL HLRSEMKSQENTL
Sbjct: 1758 QELLHLEEQKLTSIREKLNVAVRKGKSLVQQRDSLKQAIEEMTTELDHLRSEMKSQENTL 1817

Query: 1802 ASYEQNFKDFSVYSGRAEALESENLSLKNRLAEIERNFQEKEHKLSSIINTLVHIEDNVD 1861
            ASYEQ FK+FSVYSG+ EALESENLSL+N+L E ER+  EKEH LSSI NTLVHIE NVD
Sbjct: 1818 ASYEQKFKNFSVYSGQVEALESENLSLRNQLTETERSLLEKEHILSSITNTLVHIEVNVD 1877

Query: 1862 VSENDPIEKLKQVGKLCSDLREAMYFSEQESVKSRRAAELLLAELNEVQERNDTFQEELA 1921
            V+ENDPIEKLKQVGKLCSDLREA+ FSEQES+KSRRAAELLLAELNEVQERND FQEEL 
Sbjct: 1878 VNENDPIEKLKQVGKLCSDLREAVVFSEQESIKSRRAAELLLAELNEVQERNDAFQEELE 1937

Query: 1922 KASDEIAELTKERDLAETSKLEALSELEKLSTLHSKEKKIQYSQFMGLKSGLDRLKEAMR 1981
            KASDEIA LTKERDLAETSKLEALSELE LS +H KEKK Q SQFMGLKS L+R KEA+R
Sbjct: 1938 KASDEIAVLTKERDLAETSKLEALSELENLSNVHLKEKKNQISQFMGLKSNLERQKEALR 1997

Query: 1982 EINCLLADAFSKDLDAFYNLEAAIQSCTEPNGPADVNLSHSIVSGAFKSRDLKDKGKFFS 2041
            EIN LLA + SKDLDAFYNLEAAI+SCTE NGPADV  S S VSGA K    KDKG FF+
Sbjct: 1998 EINYLLAYSLSKDLDAFYNLEAAIESCTEANGPADVKPSPSFVSGALK----KDKGSFFA 2057

Query: 2042 LDSWSNFYTNAHVDENVATEIHSLV-HQLEESMKEIGALKEMIDGHSVSFHKQSDSLSKI 2101
            LDSW N Y+N+ VDENV+T+IHSL+ H LEES+KEIGALKEMIDGHSVSFHKQSDSLSK+
Sbjct: 2058 LDSWFNSYSNSPVDENVSTDIHSLIAHNLEESLKEIGALKEMIDGHSVSFHKQSDSLSKV 2117

Query: 2102 LGVLYREVNSQKELVQALKWDVQQSESVAKDREMEGDILCRNIAMLFEACTSVIKEVDER 2161
            LGVLY  VNSQKELV+ALKWDVQQSESVAKD+EMEGDILCRNIA+LFEAC S IKEVD+R
Sbjct: 2118 LGVLYSNVNSQKELVEALKWDVQQSESVAKDKEMEGDILCRNIAVLFEACISTIKEVDQR 2177

Query: 2162 KGELMGHDLTSGNLGMEIISTTPDQLSHAGKTHLLSEESVRKIADRLLLAVREFIGFKAE 2221
            KGELMG+DLTSGNLGM+IIS TPDQLS +GKTHLLSEESVR IA+RLL AVREF+G KAE
Sbjct: 2178 KGELMGNDLTSGNLGMDIISMTPDQLSRSGKTHLLSEESVRTIAERLLWAVREFLGLKAE 2237

Query: 2222 MLDGSLKEMKVSVANLQKELQEKDIQKERICMELVGQIKEAEATASRYSIDLQASKDQVY 2281
            M DGS+KEMKV+++NLQKELQEKDIQKERICM+LVGQIKEAEA+A+RYSIDLQASKDQV+
Sbjct: 2238 MFDGSVKEMKVAISNLQKELQEKDIQKERICMDLVGQIKEAEASATRYSIDLQASKDQVH 2297

Query: 2282 ELEKVTEQMDSERKVLEQRLREMQDGLSISDELRERVRLLTGSLAAKDQEIEALMHALDE 2341
            +LEK TEQM+ ERKVLEQRL+EMQDGLSISDELRERVR LT SLAAKDQEIEALM ALDE
Sbjct: 2298 KLEKATEQMEIERKVLEQRLKEMQDGLSISDELRERVRSLTDSLAAKDQEIEALMRALDE 2357

Query: 2342 EELQMEGLTKKIEDLERDLKQKNQELESIEASRGKLMKKLSITVTKFDELHHLSESLLTE 2401
            EE+QMEGLT KIE+LE+ LKQKNQELES E SRGKLMKKLSITVTKFDELH LSESLLTE
Sbjct: 2358 EEVQMEGLTNKIEELEKFLKQKNQELESTETSRGKLMKKLSITVTKFDELHQLSESLLTE 2417

Query: 2402 VEKLQAQLQDRDAEISFLRQEVTRCTNDALVATQTSNRNSEDINEVITWFDTMEARVGLS 2461
            VE+L+AQLQDRD EISFLRQEVTRCTNDA+   QTSNR++EDINE+ITWFDTME RVGLS
Sbjct: 2418 VEELRAQLQDRDDEISFLRQEVTRCTNDAIAVAQTSNRSTEDINEIITWFDTMETRVGLS 2477

Query: 2462 HSGHDDKENEVRECKEVLKKKITSILKGIEDLQAESQRKDEMLLAEKNKVEELKRKELQL 2521
            H  HD+++NEV +CKEVLKKKI SILK IEDLQA SQRKDEMLLAEKNKVEELK KELQL
Sbjct: 2478 HIVHDNQQNEVHKCKEVLKKKIASILKEIEDLQAASQRKDEMLLAEKNKVEELKCKELQL 2537

Query: 2522 NLLEDVGDNNRASSVAPEIFESEPLHIVKSCILCTLQINKWAASSTSVTPQVRSLRKGNT 2581
            NLLE+VGD NRASS  PEI ESEPL            IN WA SSTSV PQVRSLRKGNT
Sbjct: 2538 NLLEEVGDGNRASSAGPEIIESEPL------------INNWA-SSTSVIPQVRSLRKGNT 2597

Query: 2582 TDQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRIVPKFSRRMTDMIDGLWVSCDRA 2641
             DQVAIAIDMD ASSSNRLEDEDDDKVHGFKSLASSRI PKFSRR TDMIDGLWVSCDRA
Sbjct: 2598 -DQVAIAIDMDHASSSNRLEDEDDDKVHGFKSLASSRIFPKFSRRATDMIDGLWVSCDRA 2644

Query: 2642 LMRQPALRLGIIIYWAILHALVATFVV 2643
            LMRQPALRLG+I YWAILHAL+  FVV
Sbjct: 2658 LMRQPALRLGMIFYWAILHALLVAFVV 2644

BLAST of MC11g0525 vs. ExPASy TrEMBL
Match: A0A6J1FL25 (centromere-associated protein E-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446386 PE=4 SV=1)

HSP 1 Score: 3748 bits (9720), Expect = 0.0
Identity = 2083/2666 (78.13%), Postives = 2290/2666 (85.90%), Query Frame = 0

Query: 2    LQQFRKKKDNKGTGSQGNSSKNKSKLEQQDADTEIVNVAAKPTSGSYSTDGVLASSFDCD 61
            LQQFRKKKD KG GSQG+SSK+ SKLEQ DAD +         SGS+STDGVLAS+ DC 
Sbjct: 18   LQQFRKKKDYKGRGSQGSSSKHTSKLEQHDADADTATTGVL-ASGSHSTDGVLASAIDCS 77

Query: 62   ADNVDSSASPS-EHSSAAEIDHSTVYLKQEMDLAETSAIDEAEIPVEEVGYREDCDRLIQ 121
            +D VDSSASPS E S AAE+DHSTV +KQEMDLAETS ID+AE+P++EVGYRED D  IQ
Sbjct: 78   SDTVDSSASPSTELSFAAEVDHSTVSVKQEMDLAETSEIDQAEVPMQEVGYREDYDHPIQ 137

Query: 122  NAEAAGVMSSGPSILIDAEVNNNHLCNLSSTESSSQISSASVDEQGRMVEVWSGCREEEI 181
            NAE+AG  SS PS+  DAE NN+ + NLSS+ESSSQISSASV++Q ++VEVW GCR EE+
Sbjct: 138  NAESAGGRSSKPSLAPDAEGNNDDIYNLSSSESSSQISSASVEQQQKIVEVWGGCRGEEL 197

Query: 182  SPARSASLLQAREDVGMTEDASMQSDQVCETQLAEDKQLQTGGMYESAAETTFKDTHCDE 241
              + SASL QAREDVGM  D  MQS Q+C T+LAED  ++TGGM ES AETTF DT CD 
Sbjct: 198  LLSSSASLSQAREDVGMKGDGLMQSGQLCGTELAEDNLVETGGMNESTAETTFIDTCCDG 257

Query: 242  EEIITVDVASVSGFATVSNDFSISNPGENLGMQSSSSSSRDDWKEERQVHAEDMMHPSMY 301
            ++IIT DVASVSG AT SN +SIS+ GE LGMQ+SSSS R+DWKE RQVHAED +H S  
Sbjct: 258  DKIITADVASVSGAATESNSYSISSTGEKLGMQNSSSSGRNDWKEVRQVHAEDTIHSSRS 317

Query: 302  QVQYMPEDNFAVQSEGHERPSQTSAKISEGGDADAIFPNAHMTTDLAVQSGTFYSFGQES 361
            QVQYMPEDN   +SE HE PSQTS KIS+GG  D +  NAHMTT  A  SGTF SFGQ S
Sbjct: 318  QVQYMPEDNCVDKSESHESPSQTSVKISDGGHVDTLSHNAHMTTTYA-HSGTFSSFGQNS 377

Query: 362  EFLDLLERVKEELIVTSFSKDISNLQISEQNVLQMELDNQHRKLTNDMSLVNTSLNEILE 421
            +FLDLLERVKEELIVTSFSKDI N QISEQN LQ++LD                  E+L 
Sbjct: 378  KFLDLLERVKEELIVTSFSKDIFNFQISEQNELQIKLD------------------EVLV 437

Query: 422  RNQSLVDELSQCRSELRDVSSAREELKNQLLTAEAEIENLSSRATESEKNLEKFHADMFR 481
            RN +LVDELS CRSEL+DVS A EEL+NQLL AEAEI+ LSSRA+E+E + EKFH DMFR
Sbjct: 438  RNHTLVDELSHCRSELKDVSVANEELRNQLLAAEAEIQKLSSRASETEISFEKFHGDMFR 497

Query: 482  LSKELDDCKHLVTALEEENERLNGVITSENENKMKLAEEKELYINENEKISSELSSFKSL 541
            L KELDDCKHLV+ LEEENERLNG+IT ENENK KLAEEKELYI ENEKI SELSSFKSL
Sbjct: 498  LEKELDDCKHLVSVLEEENERLNGIITFENENKKKLAEEKELYIGENEKILSELSSFKSL 557

Query: 542  KVALEDENSKLIGSLSSVAEGKTKLEEEREQLFQVNGTLSVELENCRNLIATQQEEITNL 601
            K ALE ENS+L+GSLSS+AE K K EEERE LFQVNGTLSVEL NC++L+ATQQEEITNL
Sbjct: 558  KAALEVENSELMGSLSSIAEEKIKHEEEREHLFQVNGTLSVELANCKSLVATQQEEITNL 617

Query: 602  IKNLALVTEDRTKLEEDKTLLFHENEKMASELLVLDERLSAEYGERVRFEDDLRDALVRL 661
            I NLAL+TED+ +LEEDK LL HENEKM SELLVLDERLS E+ ERVRFEDDL+DA++++
Sbjct: 618  INNLALLTEDKVRLEEDKNLLLHENEKMRSELLVLDERLSTEHEERVRFEDDLKDAIMQV 677

Query: 662  EKLTEENIFLSNSLDIQKSKTEELCGEILPKQMRSIEDGNQTETADSGWHRVNKSQENDA 721
            ++LTE+N FLS+SLDI K K EELCGEIL  + RS ED +Q   ADSG H  NKSQEND+
Sbjct: 678  KQLTEDNGFLSSSLDIHKLKVEELCGEILSLKTRSREDEDQAGNADSGLHHENKSQENDS 737

Query: 722  YQ--------------------IKKQELFDDSFEFITLGRHLEEAYLVLQKLEKEIKGLQ 781
            YQ                    + +QE FDDS  F+ LGRHLEEA ++LQKLEKEIKGLQ
Sbjct: 738  YQTTFKKNLHETSVLAVGKPFIVTEQENFDDSLGFVILGRHLEEADVILQKLEKEIKGLQ 797

Query: 782  SNSASFSRSGSKVAAPAVSKLIEAFETKVN-EEHEVESEIQLPEDPYKLSSELVDNLGLL 841
            SNSASFSRSGSK+ APAVSKLI+AFE+KVN EE+EVE EIQLP DPYKLS+E VDNL  L
Sbjct: 798  SNSASFSRSGSKMDAPAVSKLIQAFESKVNVEENEVEDEIQLP-DPYKLSNEFVDNLRAL 857

Query: 842  LHQVVVDSENASVLLKGERDHKKVAISTLNKLTDQFEALENHSNDLVIANIDLGVLFEYL 901
            L QVV+D+ENASVLLKGERDH+KVAISTL++LTDQFEAL+NHSNDLVIANI+ GVLFE L
Sbjct: 858  LRQVVIDAENASVLLKGERDHQKVAISTLSELTDQFEALKNHSNDLVIANIEHGVLFECL 917

Query: 902  KHHVDDAGGKIYELENLNESLKQQGLYHKISNCELAERLRGYDLKLTELQSQLCDLHQSS 961
            KHHVDDA GKIYELE LNESL+QQG++HK SNCELAERL GY+LKLTEL+ QLCDLHQSS
Sbjct: 918  KHHVDDADGKIYELEILNESLRQQGVHHKNSNCELAERLCGYELKLTELECQLCDLHQSS 977

Query: 962  NEMVSSTCNQLDKLREGEIARATILEKDLQSFLLELAESIAKLDESLGKSDTAALKFCTN 1021
            N+MVS  CNQLD L++G I RA ILEKD  SF LELAE IAKLDESLGKSDT+A+KFCTN
Sbjct: 978  NQMVSLICNQLDNLQDGAIKRAIILEKDWHSFSLELAEIIAKLDESLGKSDTSAIKFCTN 1037

Query: 1022 DQLPSCIIASVIDAVKMIDDLRERLQATGADREAFRMLYEEVNEKYDNLFRRTEFSVDML 1081
            DQLPSC+ +SV DAVKMI DLRERLQAT AD EAFRMLYEEVNEKYDNLFRRTE SVD+L
Sbjct: 1038 DQLPSCLASSVTDAVKMIHDLRERLQATAADGEAFRMLYEEVNEKYDNLFRRTECSVDLL 1097

Query: 1082 HRIYSELQKLYIASCGSVGGSDMNMQIKGLGDPLDYSSFEAFIKLLEDCITERLQLESVN 1141
            H+I+ ELQKLY+ASC SVGGSDMNMQIK LGDPLDYSSFEA IK LEDCIT+RLQLESVN
Sbjct: 1098 HKIFGELQKLYLASCESVGGSDMNMQIKMLGDPLDYSSFEALIKPLEDCITQRLQLESVN 1157

Query: 1142 NKLRLDLEHMNVGFVDFSKRCLDSPGIKKLITDVQSVLLLDDAEMDV-EMPALYLESMVS 1201
            NKLRLDLEH  V FV+FSKRCLDS GI+KLI DVQ VLL +D E D  +MPALYLES++S
Sbjct: 1158 NKLRLDLEHRTVEFVEFSKRCLDSTGIEKLIKDVQGVLLPEDTEGDCGQMPALYLESIIS 1217

Query: 1202 LLLQKYKETELQLGLSREKSGSLMMKLTELQESVHDLSTLILDHECEIVILKESLSQVQE 1261
            LL+QKYK+TEL+LGLSRE+ GS MMKLTELQ SVHDLSTLIL HE EIVILKESLSQ QE
Sbjct: 1218 LLIQKYKDTELRLGLSREEYGSAMMKLTELQGSVHDLSTLILGHEGEIVILKESLSQAQE 1277

Query: 1262 ALVASRSVLKDKANELEQSEQRVSAIREKLSIAVAKGKGLIVQRDNLKQSLAQTSSELER 1321
            AL+ASRS LKDK NELEQSEQRVSAIREKLSIAVAKGKGLIVQRD LKQSLAQTSSELER
Sbjct: 1278 ALMASRSELKDKLNELEQSEQRVSAIREKLSIAVAKGKGLIVQRDGLKQSLAQTSSELER 1337

Query: 1322 CLQELQMKDTRLHETETKLTAYSEAGERVEALESELSYIRNSATALRESFLLKDSVLQRI 1381
            CLQELQMKD RL ETETKL  YSEAGERVEALESEL YIRNSATALRESFLLKDSVLQRI
Sbjct: 1338 CLQELQMKDNRLLETETKLKTYSEAGERVEALESELLYIRNSATALRESFLLKDSVLQRI 1397

Query: 1382 EEILDELDLPENFHSRDIIEKVDWLAKSSTGENLPHTDWDQRSSVAGGSGSDANFVIMDA 1441
            +EILD LDLPENFHS DIIEKVDWLAKSSTG+NLP TD DQRSSV GGSGSDANFV  D 
Sbjct: 1398 DEILDALDLPENFHSTDIIEKVDWLAKSSTGKNLPQTDGDQRSSVTGGSGSDANFVTTDG 1457

Query: 1442 WKDEVQMDANVGDDLRRKYEELQTRFYGLAEQNEMLEQSLMERNNIVQRWEELLEKIDIP 1501
            WKDE+Q DANVGDDL R+YEELQT+FYGLAEQNEMLEQSLMERNN+VQRWEELLEKID P
Sbjct: 1458 WKDEMQTDANVGDDLIRQYEELQTKFYGLAEQNEMLEQSLMERNNLVQRWEELLEKIDTP 1517

Query: 1502 SQLRSMEPEDKMEWLHKSLSEAFHDRDSLHQRVNYLENYCGLLTADLDDSQKKISDLEAE 1561
            S LRS+EPEDK+EWLH+SL+EA HDRDSLHQRVN LEN+CGLLTADLDDS+KKIS +EAE
Sbjct: 1518 SHLRSIEPEDKIEWLHRSLTEACHDRDSLHQRVNNLENHCGLLTADLDDSRKKISGIEAE 1577

Query: 1562 LHSLVLEREKLSEKLEIIYHHNENLAFGNFENEVGIIVLQNELSNMQENLISTEHKIVKL 1621
            LHS++LEREKLSEKLEI+Y HNE+L+F  FENEV II+LQNE  N+Q+ +ISTEHKI+KL
Sbjct: 1578 LHSVMLEREKLSEKLEIVYDHNEHLSFVTFENEVEIIILQNESRNIQDKIISTEHKILKL 1637

Query: 1622 EALVSNALQDEDVNDLVSGSSIEFLDLMVTKLIQNYTASSSGKAVLGKATNGHDTEEEML 1681
            EALVSNAL+D D+NDLVSGS IE L+LMV KL+QNYTASS G   LG+ATNG D EE ++
Sbjct: 1638 EALVSNALRDMDMNDLVSGSGIESLELMVMKLVQNYTASSLGNVELGRATNGPDAEE-IV 1697

Query: 1682 ARSKNMHDAWQNDINILKKELEDALNQLMVVTEERDQYMEMHESLVVKVESSDKKKDELQ 1741
             RS +    WQNDIN  KKELEDA++QLMVVT+ERDQYMEMHESLVVKVES D+KKDELQ
Sbjct: 1698 PRSIDTQVGWQNDINDHKKELEDAVHQLMVVTKERDQYMEMHESLVVKVESLDRKKDELQ 1757

Query: 1742 ELLNLEEQKSASIREKLNVAVRKGKSLIQQRDSLKQAVEEMTTELKHLRSEMKSQENTLA 1801
            ELL+LEEQKS SIREKLNVAVRKGKSL+QQRDSLKQA+EEMTTEL HLRS+MKSQENTLA
Sbjct: 1758 ELLHLEEQKSTSIREKLNVAVRKGKSLVQQRDSLKQAIEEMTTELDHLRSKMKSQENTLA 1817

Query: 1802 SYEQNFKDFSVYSGRAEALESENLSLKNRLAEIERNFQEKEHKLSSIINTLVHIEDNVDV 1861
            SYEQ FK+FSVYSGR EALESENLSL+N+L E E +  EKEH LSSI NTLVHIE N D 
Sbjct: 1818 SYEQKFKNFSVYSGRVEALESENLSLRNQLTETESSLLEKEHILSSITNTLVHIEVNDDA 1877

Query: 1862 SENDPIEKLKQVGKLCSDLREAMYFSEQESVKSRRAAELLLAELNEVQERNDTFQEELAK 1921
            +ENDPIEKLKQVGKLCSDLREA+  SEQES+KSRRAAELLLAELNEVQERND FQEEL K
Sbjct: 1878 NENDPIEKLKQVGKLCSDLREAVVSSEQESIKSRRAAELLLAELNEVQERNDAFQEELEK 1937

Query: 1922 ASDEIAELTKERDLAETSKLEALSELEKLSTLHSKEKKIQYSQFMGLKSGLDRLKEAMRE 1981
            ASDEIA LTKERDLAETSKLEALSELE LS +H KEKK Q SQFMGLKS  +R KEA+RE
Sbjct: 1938 ASDEIAVLTKERDLAETSKLEALSELENLSNVHLKEKKNQISQFMGLKSNFERQKEALRE 1997

Query: 1982 INCLLADAFSKDLDAFYNLEAAIQSCTEPNGPADVNLSHSIVSGAFKSRDLKDKGKFFSL 2041
            IN LLAD+ SKDLDAFYNLEAAI SCTE NGPADVN S SIVSGA K    KDKG FF+L
Sbjct: 1998 INYLLADSLSKDLDAFYNLEAAIMSCTEANGPADVNPSPSIVSGALK----KDKGSFFAL 2057

Query: 2042 DSWSNFYTNAHVDENVATEIHSLV-HQLEESMKEIGALKEMIDGHSVSFHKQSDSLSKIL 2101
            DSW N Y+N+ VDENV+T+IHSL+ H LEES+KEIGALKEMIDGHSVSFHKQSDSLSK+L
Sbjct: 2058 DSWFNSYSNSPVDENVSTDIHSLIAHHLEESLKEIGALKEMIDGHSVSFHKQSDSLSKVL 2117

Query: 2102 GVLYREVNSQKELVQALKWDVQQSESVAKDREMEGDILCRNIAMLFEACTSVIKEVDERK 2161
            GVLY  VNSQKELV+ALKWDVQQSESVAKD+EMEGDILCRNIA+LFEAC S IKEVD+RK
Sbjct: 2118 GVLYSNVNSQKELVEALKWDVQQSESVAKDKEMEGDILCRNIAVLFEACISTIKEVDQRK 2177

Query: 2162 GELMGHDLTSGNLGMEIISTTPDQLSHAGKTHLLSEESVRKIADRLLLAVREFIGFKAEM 2221
            GELMG+ LTSGNLGM+IIS TPDQLS +GKTHLLSEESVR IADRLL AVREFIG KAEM
Sbjct: 2178 GELMGNYLTSGNLGMDIISMTPDQLSRSGKTHLLSEESVRTIADRLLWAVREFIGLKAEM 2237

Query: 2222 LDGSLKEMKVSVANLQKELQEKDIQKERICMELVGQIKEAEATASRYSIDLQASKDQVYE 2281
             DGS+KEMKV+++NLQKELQEKDIQKERICM+LVGQIKEAEA+A+RYSIDLQASKDQV++
Sbjct: 2238 FDGSVKEMKVAISNLQKELQEKDIQKERICMDLVGQIKEAEASATRYSIDLQASKDQVHK 2297

Query: 2282 LEKVTEQMDSERKVLEQRLREMQDGLSISDELRERVRLLTGSLAAKDQEIEALMHALDEE 2341
            LEK TEQM+ ERKVLEQRLREMQDG SISDELRERVR LT SLAAKDQEIEALM ALDEE
Sbjct: 2298 LEKATEQMEIERKVLEQRLREMQDGFSISDELRERVRSLTDSLAAKDQEIEALMRALDEE 2357

Query: 2342 ELQMEGLTKKIEDLERDLKQKNQELESIEASRGKLMKKLSITVTKFDELHHLSESLLTEV 2401
            E+QMEGLT KIE+LE+ LKQKNQELES E SRGKLMKKLSITVTKFDELHHLSESLLTEV
Sbjct: 2358 EVQMEGLTNKIEELEKFLKQKNQELESTETSRGKLMKKLSITVTKFDELHHLSESLLTEV 2417

Query: 2402 EKLQAQLQDRDAEISFLRQEVTRCTNDALVATQTSNRNSEDINEVITWFDTMEARVGLSH 2461
            E+L+AQLQDRD EISFLRQEVTRCTNDA+  TQTSNR++EDINE+ITWFDTME RVGLSH
Sbjct: 2418 EELRAQLQDRDGEISFLRQEVTRCTNDAIAVTQTSNRSTEDINEIITWFDTMETRVGLSH 2477

Query: 2462 SGHDDKENEVRECKEVLKKKITSILKGIEDLQAESQRKDEMLLAEKNKVEELKRKELQLN 2521
              HDD++NEV ECKEVLKKKITSILK IEDL+A SQRKDEMLLAEK+KVEELK KELQLN
Sbjct: 2478 IIHDDQQNEVHECKEVLKKKITSILKEIEDLKAASQRKDEMLLAEKHKVEELKCKELQLN 2537

Query: 2522 LLEDVGDNNRASSVAPEIFESEPLHIVKSCILCTLQINKWAASSTSVTPQVRSLRKGNTT 2581
            LLEDVGD NRASSV PEI ESEPL            IN WA SSTSV PQVRSLRKGNT 
Sbjct: 2538 LLEDVGDGNRASSVGPEIIESEPL------------INNWA-SSTSVIPQVRSLRKGNT- 2597

Query: 2582 DQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRIVPKFSRRMTDMIDGLWVSCDRAL 2641
            DQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRI PKFSRR TDMIDGLWVSCDRAL
Sbjct: 2598 DQVAIAIDMDPASSSNRLEDEDDDKVHGFKSLASSRIFPKFSRRATDMIDGLWVSCDRAL 2643

Query: 2642 MRQPALRLGIIIYWAILHALVATFVV 2643
            MRQPALRLG+I YWAILHAL+  FVV
Sbjct: 2658 MRQPALRLGMIFYWAILHALLVAFVV 2643

BLAST of MC11g0525 vs. TAIR 10
Match: AT4G31570.1 (CONTAINS InterPro DOMAIN/s: Prefoldin (InterPro:IPR009053); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G24460.1); Has 194354 Blast hits to 66887 proteins in 3244 species: Archae - 3688; Bacteria - 38556; Metazoa - 84828; Fungi - 17265; Plants - 10589; Viruses - 805; Other Eukaryotes - 38623 (source: NCBI BLink). )

HSP 1 Score: 1371.7 bits (3549), Expect = 0.0e+00
Identity = 1035/2816 (36.75%), Postives = 1550/2816 (55.04%), Query Frame = 0

Query: 1    QLQQFR---------KKKDNKGTGSQGNSSKNKSKLEQQDADTEIVNVAAKPTSGSYSTD 60
            +LQQFR         +KKD+KG+ SQG SSK  +K E+ +   +   V+ +  + S  T 
Sbjct: 17   KLQQFRQKKADKGTDQKKDSKGSTSQGKSSKKSNKSEKHERKPDTSAVSDEAQAPSPVTV 76

Query: 61   GVLASSFDCDADNVDSSASPSEHSSAAEID-HSTVYLKQEMDLAETSAIDEAEIPVEEVG 120
            G   S  +   + VDS  + S+  +   +  H +      +    T++ D +E   E V 
Sbjct: 77   GGATSHVNVAEEVVDSPQTSSDTKAHEYVSVHGSSSEPDALQPGHTTSNDGSEARKEVVN 136

Query: 121  YREDCDRLIQ---------NAEAAGVMSSGPSILIDAEVNNNHLCNLSSTESSSQISSAS 180
               D  + +          N+  AG + S  S   D+E    H  + S+ +     S   
Sbjct: 137  SENDISKSLSTEEENVKSINSGVAGTVDSLISDPADSEKGVTH-DDASNVDGIFAASGNI 196

Query: 181  VDEQGRMVEVWSGCREEEISPAR------SASLLQAR----EDVGMTEDASMQSDQVCET 240
             + +G  VE  SG  E+   P+         SL++AR     DVG  ++  M+       
Sbjct: 197  AEGEGVEVEGGSGNVEKPHQPSSLQEYIPDVSLIRARGDQVTDVGEMQEEDMEQFSELSA 256

Query: 241  QLAEDK----QLQTGGMYESAAETTFKDTHCDEEEIITVDVASVSGFATVSNDFSISNPG 300
            +   DK    + QT   Y +  +++   +H  E   +  D   + G   ++ +F      
Sbjct: 257  KAGVDKIATEERQTS--YPAVVDSSASPSHFSEGSSVAFDTVELEG---INGNFRSQQIR 316

Query: 301  ENLGMQSSSSSSRDDWKEER----QVHAEDMMHPSMYQVQYMPEDNFAVQSEGHERPSQ- 360
            E   +      +  D+   R        E+     M     +PE         HE   + 
Sbjct: 317  EAAELNEEKPETSIDFPNNRDHVLSAEPEESSVAEMASQLQLPESVSISGVLSHEETRKI 376

Query: 361  ---------TSAKISEGGDAD---------AIFPNAHMTTDLAVQSGTFYSFGQESEFLD 420
                     TSA + EG              +  + +     A ++ +    G  S    
Sbjct: 377  DTLNLSAELTSAHVHEGRSVSFLQLMDIVKGLGQDEYQILCNAREAASSTEPGTSS---- 436

Query: 421  LLERVKEELIVTSFSKDISNLQISEQNVLQMELDNQHRKLTNDMSLVNTSLNEILERNQS 480
             LER++EEL V+S  +DI ++Q++EQ+ LQ+E D+QH +   ++S +  S + + ERN S
Sbjct: 437  -LERLREELFVSSTMEDILHVQLTEQSHLQIEFDHQHNQFVAEISQLRASYSAVTERNDS 496

Query: 481  LVDELSQCRSELRDVSSAREELKNQLLTAEAEIENLSSRATESEKNLEKFHADMFRLSKE 540
            L +ELS+C+S+L   +S+   L+NQLL  EA++E+ +++  E + +LEK   D       
Sbjct: 497  LAEELSECQSKLYAATSSNTNLENQLLATEAQVEDFTAKMNELQLSLEKSLLD------- 556

Query: 541  LDDCKHLVTALEEENERLNGVITSENENKMKLAEEKELYINENEKISSELSSFKSLKVAL 600
            L + K     L+ EN+ L  VI+S N+ K +L EEKE    E + +SSEL + K+L   L
Sbjct: 557  LSETKEKFINLQVENDTLVAVISSMNDEKKELIEEKESKNYEIKHLSSELCNCKNLAAIL 616

Query: 601  EDENSKLIGSLSSVAEGKTKLEEEREQLFQVNGTLSVELENCRNLIATQQEEITNLIKNL 660
            + E  +   ++  + + K  L EE+  L      L  EL NC+ ++  Q+ E +N+ + L
Sbjct: 617  KAEVEQFENTIGPLTDEKIHLVEEKYSLLGEAEKLQEELANCKTVVTLQEVENSNMKETL 676

Query: 661  ALVTEDRTKLEE------------------------------------------------ 720
            +L+T  +T  EE                                                
Sbjct: 677  SLLTRQQTMFEENNIHLREENEKAHLELSAHLISETYLLSEYSNLKEGYTLLNNKLLKFQ 736

Query: 721  -DKTLLFHENEKMASELLVLDERLSAEYGERVRFEDDLRDALVRLEKLTEENIFLSNSLD 780
             +K  L  EN+K+  ELL L E +S    ER   E +LR+A+ RL+KL EEN  L++S+ 
Sbjct: 737  GEKEHLVEENDKLTQELLTLQEHMSTVEEERTHLEVELREAIARLDKLAEENTSLTSSIM 796

Query: 781  IQKSK-----TEELCG----EILPKQMRSIEDGNQTETADSGWHRVNKSQENDAYQIKKQ 840
            ++K++     + ++ G    EI  K  RS E G   ++A           EN  Y    +
Sbjct: 797  VEKARMVDNGSADVSGLINQEISEKLGRSSEIGVSKQSA--------SFLENTQY-TNLE 856

Query: 841  ELFDDSFEFITLGRHLEEAYLVLQKLEKEIKGLQSNSASFSRSGSKVAAPAVSKLIEAFE 900
            E+ + + EF  L ++LE+   ++Q LE+ IK + ++S S S+S  K A PAVSKLI+AFE
Sbjct: 857  EVREYTSEFSALMKNLEKGEKMVQNLEEAIKQILTDS-SVSKSSDKGATPAVSKLIQAFE 916

Query: 901  TKVNEEHEVESEIQLPE-----DPYKLSSELVDNLGLLLHQVVVDSENASVLLKGERDHK 960
            +K   E       QL +     D +   +  + NL  LL Q+++++  A +      D +
Sbjct: 917  SKRKPEEPESENAQLTDDLSEADQFVSVNVQIRNLRGLLDQLLLNARKAGIQFNQLNDDR 976

Query: 961  KVAISTLNKLTDQFEALENHSNDLVIANIDLGVLFEYLKHHVDDAGGKIYELENLNESLK 1020
                  L +L  +F + ++H N L    I+  V FE LKH+  +   K ++LE L +SLK
Sbjct: 977  TSTNQRLEELNVEFASHQDHINVLEADTIESKVSFEALKHYSYELQHKNHDLELLCDSLK 1036

Query: 1021 QQGLYHKISNCELAERLRGYDLKLTELQSQLCDLHQSSNEMVSSTCNQLDKLREGEIARA 1080
             +     + N EL ++L    L++ EL+ QL +L Q+    +S+   QL  L++ E  RA
Sbjct: 1037 LRNDNISVENTELNKKLNYCSLRIDELEIQLENLQQNLTSFLSTMEEQLVALQD-ESERA 1096

Query: 1081 TILEKDLQSFLLELAESIAKLDESLGKSDTAALKFCTNDQLPSCIIASVIDAVKMIDDLR 1140
             ++E +L S + E  E++ +LD+ L +S T+     T   +   I  SV  AV +I+DL+
Sbjct: 1097 MMVEHELTSLMSEFGEAVVRLDDCLLRSGTSGAH--TGLDMTKRISGSVDVAVNVIEDLK 1156

Query: 1141 ERLQATGADREAFRMLYEEVNEKYDNLFRRTEFSVDMLHRIYSELQKLYIASCGSVGGSD 1200
            E+L+A     E+    YEE+ + ++ LF + EF+   + ++Y++L KL   SCGS   + 
Sbjct: 1157 EKLEAAYVKHESTSNKYEELKQSFNTLFEKNEFTASSMQKVYADLTKLITESCGSAEMTS 1216

Query: 1201 MNMQIKGLGDPLDYSSFEAFIKLLEDCITERLQLESVNNKLRLDLEHMNVGFVDFSKRCL 1260
            + ++   + DP    SFE  ++ +   ++ERL+L+SV +KL+ DL   +    + ++R L
Sbjct: 1217 LEVENVAVFDPFRDGSFENLLEAVRKILSERLELQSVIDKLQSDLSSKSNDMEEMTQRSL 1276

Query: 1261 DSPGIKKLITDVQSVLLLDDAEMDVEMPALYLESMVSLLLQKYKETELQLGLSREKSGSL 1320
            DS  +++L+  V+ +L L+   +  E P+  +E +VS L+QK+ E E    L R++  + 
Sbjct: 1277 DSTSLRELVEKVEGLLELESGVI-FESPSSQVEFLVSQLVQKFIEIEELANLLRKQLEAK 1336

Query: 1321 MMKLTELQESVHDLSTLILDHECEIVILKESLSQVQEALVASRSVLKDKANELEQSEQRV 1380
              +L E++ES       +L H+ +I  L+ESL+Q +E+LVA RS L+DK+NELEQSEQR+
Sbjct: 1337 GNELMEIEES-------LLHHKTKIAGLRESLTQAEESLVAVRSELQDKSNELEQSEQRL 1396

Query: 1381 SAIREKLSIAVAKGKGLIVQRDNLKQSLAQTSSELERCLQELQMKDTRLHETETKLTAYS 1440
             + REKLSIAV KGKGLIVQRDN+KQSLA+ S++L++C +EL  KD RL E E KL  Y 
Sbjct: 1397 LSTREKLSIAVTKGKGLIVQRDNVKQSLAEASAKLQKCSEELNSKDARLVEVEKKLKTYI 1456

Query: 1441 EAGERVEALESELSYIRNSATALRESFLLKDSVLQRIEEILDELDLPENFHSRDIIEKVD 1500
            EAGERVEALESELSYIRNSATALRESFLLKDS+L RIEEIL++LDLPE+FH+RDI+EKV+
Sbjct: 1457 EAGERVEALESELSYIRNSATALRESFLLKDSLLHRIEEILEDLDLPEHFHARDILEKVE 1516

Query: 1501 WLAKSSTGENLPHTDWDQRSSVAGGSGSDANFVIMDAWKDEVQMDANVGDDLRRKYEELQ 1560
            WLA+S+ G +   + WDQ+SS  G     A FV+ + W+++VQ   +  DDLR K+EEL+
Sbjct: 1517 WLARSANGNSSRPSGWDQKSSDGG-----AGFVLSEPWREDVQTGTSSEDDLRIKFEELK 1576

Query: 1561 TRFYGLAEQNEMLEQSLMERNNIVQRWEELLEKIDIPSQLRSMEPEDKMEWLHKSLSEAF 1620
             +FYGLAEQNEMLEQSLMERN +VQRWE+LLE IDIP QL SME E+K+EWL  +++EA 
Sbjct: 1577 GKFYGLAEQNEMLEQSLMERNTLVQRWEKLLENIDIPPQLHSMEVENKIEWLASTITEAT 1636

Query: 1621 HDRDSLHQRVNYLENYCGLLTADLDDSQKKISDLEAELHSLVLEREKLSEKLEIIYHHNE 1680
            HDRD+L Q+++ LE YC  +T DL+ SQK++ D+E  L S V ER  LSE+LE +   +E
Sbjct: 1637 HDRDNLQQKIDNLEVYCQSVTTDLEVSQKQVGDVEGNLQSCVSERVNLSERLESLIGDHE 1696

Query: 1681 NLAFGNFENEVGIIVLQNELSNMQENLI----------STEHKIVKLEALVSNALQDEDV 1740
            +L+      EV    LQN++ ++ E L+          + E  ++ L  ++ + +Q++ +
Sbjct: 1697 SLSARGIHLEVENEKLQNQVKDLHEKLVEKLGNEEHFQTIEGDLLSLRYMIDDVIQEDGL 1756

Query: 1741 NDLVSGSSIEFLDLMVTKLIQNY------------------TASSSGKAVLGKATNGHDT 1800
             DL   S+ E LD ++ KLI  Y                  T  S      G++   H  
Sbjct: 1757 QDLALASNSENLDGVLRKLIDYYKNLVKSSLPGETDDNVCETRPSDADVRSGESLGAHGA 1816

Query: 1801 E---EEMLARSKNMHDAWQNDINI--------LKKELEDALNQLMVVTEERDQYMEMHES 1860
                +       N+ +A   DI +        L K+L+ AL+   +  EERD YM   +S
Sbjct: 1817 TSHGQHFELSDSNVVEATSRDIAVVETPDVASLTKDLDQALHVQKLTREERDLYMAKQQS 1876

Query: 1861 LVVKVESSDKKKDELQELLNLEEQKSASIREKLNVAVRKGKSLIQQRDSLKQAVEEMTTE 1920
            LV + E+ DKK  ELQE L  EEQKSAS+REKLNVAVRKGK+L+QQRDSLKQ +EE+  E
Sbjct: 1877 LVAENEALDKKIIELQEFLKQEEQKSASVREKLNVAVRKGKALVQQRDSLKQTIEEVNAE 1936

Query: 1921 LKHLRSEMKSQENTLASYEQNFKDFSVYSGRAEALESENLSLKNRLAEIERNFQEKEHKL 1980
            L  L+SE+  ++  L   E+ F++   YS R E+LESE   LK    E E   QE+   L
Sbjct: 1937 LGRLKSEIIKRDEKLLENEKKFRELESYSVRVESLESECQLLKIHSQETEYLLQERSGNL 1996

Query: 1981 SSIINTLVHIEDNVDVSENDPIEKLKQVGKLCSDLREAMYFSEQESVKSRRAAELLLAEL 2040
            S  +N L  I+   +   NDP+ KL+++ +L   +   +  +EQES KSRRAAELLLAEL
Sbjct: 1997 SMTLNALNSIDIGDEGDINDPVMKLQRISQLFQTMSTTVTSAEQESRKSRRAAELLLAEL 2056

Query: 2041 NEVQERNDTFQEELAKASDEIAELTKERDLAETSKLEALSELEKLSTLHSKEKKIQYSQF 2100
            NEVQE ND+ QE+L+K + EI +L++E+D AE +K+EA+S  E LS + ++EK   Y+Q 
Sbjct: 2057 NEVQETNDSLQEDLSKFTYEIQQLSREKDAAEAAKVEAISRFENLSAVSNEEKNKLYAQL 2116

Query: 2101 MGLKSGLDRLKEAMREINCLLADAFSKDLDAFYNLEAAIQSCTEPNGPADVNLSHSIVSG 2160
            +   + ++ L++ +   N  LAD F  D++  ++L+A ++ C +  G     L       
Sbjct: 2117 LSCGTSVNSLRKILAGTNSCLADIFIMDMEFLHHLKANMELCAKKTGTDLSGLPQ----- 2176

Query: 2161 AFKSRDLKDKGKFFSLD-SWSNFYTNAHVDE---NVATEIHSLVHQLEESMKEIGALKEM 2220
               + +L DK  F  L  +WSN   N H      N+A    SL   L++ +  +  L+E 
Sbjct: 2177 -LSTENLVDKEIFARLSAAWSNI--NLHETSSGGNIAEICGSLSQNLDQFVVGVSHLEEK 2236

Query: 2221 IDGHSVSFHKQSDSLSKILGVLYREVNSQKELVQALKWDVQQSESVAKDREMEGDILCRN 2280
            +  H  ++H Q + +S  +   ++                    S+    + E   L   
Sbjct: 2237 VSKHLATWHDQINIVSNSIDTFFK--------------------SIGTGTDSEVAALGER 2296

Query: 2281 IAMLFEACTSVIKEVDERKGELMGHDLTSGNLGMEIISTTPDQLSHAGKTHLLSEESVRK 2340
            IA+L  AC+SV+ E++ RK EL+G+D    +  M +         H       S ESVR 
Sbjct: 2297 IALLHGACSSVLVEIERRKAELVGND----DFNMSL---------HQVDEDFSSMESVRS 2356

Query: 2341 IADRLLLAVREFIGFKAEMLDGSLKEMKVSVANLQKELQEKDIQKERICMELVGQIKEAE 2400
            + +RL  AV+E +   AE L+ + KEMKV +ANLQ+EL EKDIQ  R C ELVGQ+KEA+
Sbjct: 2357 MVNRLSSAVKELVVANAETLERNEKEMKVIIANLQRELHEKDIQNNRTCNELVGQVKEAQ 2416

Query: 2401 ATASRYSIDLQASKDQVYELEKVTEQMDSERKVLEQRLREMQDGLSISDELRERVRLLTG 2460
            A A  ++ DLQ++  ++ +++     +  ER  +++R++E+  G +   EL+E+V  L+ 
Sbjct: 2417 AGAKIFAEDLQSASARMRDMQDQLGILVRERDSMKERVKELLAGQASHSELQEKVTSLSD 2476

Query: 2461 SLAAKDQEIEALMHALDEEELQMEGLTKKIEDLERDLKQKNQELESIEASRGKLMKKLSI 2520
             LAAKD EIEALM ALDEEE QME L  ++ +LE++++QKN +L+  EASRGK+ KKLSI
Sbjct: 2477 LLAAKDLEIEALMQALDEEESQMEDLKLRVTELEQEVQQKNLDLQKAEASRGKISKKLSI 2536

Query: 2521 TVTKFDELHHLSESLLTEVEKLQAQLQDRDAEISFLRQEVTRCTNDALVATQT-SNRNSE 2580
            TV KFDELHHLSE+LL E+EKLQ Q+QDRD E+SFLRQEVTRCTN+AL A+Q  + R+SE
Sbjct: 2537 TVDKFDELHHLSENLLAEIEKLQQQVQDRDTEVSFLRQEVTRCTNEALAASQMGTKRDSE 2596

Query: 2581 DINEVITWFDTMEARVGLSHSGHDDKENEVRECKEVLKKKITSILKGIEDLQAESQRKDE 2640
            +I  V++WFDT+ + +G+  S   D ++ +    E  +K+I S+L  I++L+   Q KD 
Sbjct: 2597 EIQTVLSWFDTIASLLGIEDSLSTDADSHINHYMETFEKRIASMLSEIDELRLVGQSKDV 2656

Query: 2641 MLLAEKNKVEELKRKELQLN--LLEDVGDNNRASSVAPEIFESEPLHIVKSCILCTLQIN 2644
            +L  E+++V EL++KE  L   LLE     + ++S   EI E EPL            IN
Sbjct: 2657 LLEGERSRVAELRQKEATLEKFLLEKESQQDISTSSTSEIVEVEPL------------IN 2716

BLAST of MC11g0525 vs. TAIR 10
Match: AT1G24460.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G31570.1). )

HSP 1 Score: 167.2 bits (422), Expect = 1.8e-40
Identity = 348/1613 (21.57%), Postives = 669/1613 (41.48%), Query Frame = 0

Query: 1172 YLESMVSLLLQKYKE-----TELQ-------LGLSREKS-----GSLMMKLTELQESVHD 1231
            +LE+ VS L  KY E      +L+       L LS ++      G+   +L EL++    
Sbjct: 212  HLENRVSFLGAKYTEFYYGADQLRKCLASDVLDLSFQEDFGSALGAACSELFELKQKEAA 271

Query: 1232 LSTLILDHECEIVILKESLSQVQEALVASRSVLKDKANELEQSEQRVSAIREKLSIAVAK 1291
                +   E E     E +++ +E   + R+  +    ELE  + + +  +EKLS+AV K
Sbjct: 272  FFERLSHLEDENRNFVEQVNREKEMCESMRTEFEKLKAELELEKTKCTNTKEKLSMAVTK 331

Query: 1292 GKGLIVQRDNLKQSLAQTSSELERCLQELQMKDTRLHETET-KLTAYSEAGERVEALESE 1351
            GK L+  RD LK  L++ ++EL   L ELQ K+  L  +E  K        E+ + LE  
Sbjct: 332  GKALVQNRDALKHQLSEKTTELANRLTELQEKEIALESSEVMKGQLEQSLTEKTDELEKC 391

Query: 1352 LSYIRNSATALRESFLLKDSVLQRIEEILDELDLPENFHSRDIIEKVDWLAKSSTGENLP 1411
             + + + + +L    L K  + Q + E   EL+           E +  L + ST  +  
Sbjct: 392  YAELNDRSVSLEAYELTKKELEQSLAEKTKELE-----------ECLTKLQEMSTALDQS 451

Query: 1412 HTDWDQRSSVAGGSGSDANFVIMDAWKDEVQMDANVGDDLRRKYEELQTRFYGLAEQNEM 1471
              D   +  +A      A++  M + ++ +    N+   L   Y   +   + + E+   
Sbjct: 452  ELD---KGELAKSDAMVASYQEMLSVRNSI--IENIETILSNIYTPEEGHSFDIVEKVRS 511

Query: 1472 LEQSLMERNNIVQ---RWEELLEKIDIPSQLRSMEPEDKMEWLHKSLSEAFHDRDSLHQR 1531
            L +   E  N+ Q   R ++L+  ID+P ++     E ++ WL +S  +    +D ++  
Sbjct: 512  LAEERKELTNVSQEYNRLKDLIVSIDLPEEMSQSSLESRLAWLRESFLQG---KDEVNAL 571

Query: 1532 VNYLENYCGLLTADLDDSQKKISDLEAELHSLVLEREKLSEKLEIIYHHNENLAFGNFEN 1591
             N +E+    L+A++++     S++  EL  L    +K+ E  E           G+ E 
Sbjct: 572  QNRIESVSMSLSAEMEEK----SNIRKELDDLSFSLKKMEETAE----------RGSLER 631

Query: 1592 EVGIIVLQNELSNMQENLISTEHKIVKLEALVSNALQD---EDVNDLVSGSSIEFLDLMV 1651
            E                      ++V+   L++  ++D    D+N LV  S  +     +
Sbjct: 632  E------------------EIVRRLVETSGLMTEGVEDHTSSDINLLVDRSFDK-----I 691

Query: 1652 TKLIQNYTASSSGKAVLGKATNG----HDTEEEMLARSKNMHDAWQNDINILKKELEDAL 1711
             K I++ + SS G   + +A        D E  +        +     ++ L  EL+ A 
Sbjct: 692  EKQIRDSSDSSYGNEEIFEAFQSLLYVRDLEFSLCKEMLGEGELISFQVSNLSDELKIAS 751

Query: 1712 NQLMVVTEERDQYMEMHESLVVKVESSDKKKDELQELLNLEEQKSASIREKLNVAVRKGK 1771
             +L  V EE                     K  L++ L   E+KSA +R+KL++A++KGK
Sbjct: 752  QELAFVKEE---------------------KIALEKDLERSEEKSALLRDKLSMAIKKGK 811

Query: 1772 SLIQQRDSLKQAVEEMTTELKHLRSEMKSQENTLASYEQNFKDFSVYSGRAEALESENLS 1831
             L+Q R+  K  ++E  +E++ L  E++    T+  Y+      S    R + LE+E ++
Sbjct: 812  GLVQDREKFKTQLDEKKSEIEKLMLELQQLGGTVDGYKNQIDMLSRDLERTKELETELVA 871

Query: 1832 LKNRLAEIERNFQEKEHKLSSIINTLVHIEDNVDVSENDPIEKLKQVGKLCSDLREAMYF 1891
             K    +++++    +  L  ++ ++  I   VD++  DP EK+ ++     +++ A   
Sbjct: 872  TKEERDQLQQSLSLIDTLLQKVMKSVEIIALPVDLASEDPSEKIDRLAGYIQEVQLARVE 931

Query: 1892 SEQESVKSRRAAELLLAELNEVQERNDTFQEELAKASDEIAELTKERDLAETSKLEALSE 1951
             ++E  K +   + L ++L E Q      ++ L+ A D I+ LT+E    + +K  A  E
Sbjct: 932  EQEEIEKVKSEVDALTSKLAETQTALKLVEDALSTAEDNISRLTEENRNVQAAKENAELE 991

Query: 1952 LEKLSTLHSKEKKIQYSQFMGLKSGLD-RLKEAMREINCLLAD--------AFSKDLDAF 2011
            L+K +   +     +  + +  KS L+  L +A R I+ ++++        A ++     
Sbjct: 992  LQK-AVADASSVASELDEVLATKSTLEAALMQAERNISDIISEKEEAQGRTATAEMEQEM 1051

Query: 2012 YNLEAAIQSCTEPNGPADVNLSHSIVSGAFKSRDL------KDKGKFFSL-DSWSNFYTN 2071
               EA+IQ        + +N     ++    + D        DK    SL +        
Sbjct: 1052 LQKEASIQKNKLTEAHSTINSLEETLAQTESNMDSLSKQIEDDKVLTTSLKNELEKLKIE 1111

Query: 2072 AHVDENVATEIHSLVHQLEESMKEIGALKEMIDGHSVSFHKQSDSLSKILGVLYREV--- 2131
            A  + N   E    +   EE++ +       + G  V    +  +LS  L V   E+   
Sbjct: 1112 AEFERNKMAEASLTIVSHEEALMKAENSLSALQGEMVKAEGEISTLSSKLNVCMEELAGS 1171

Query: 2132 --NSQKE-------------------LVQALKWDVQQSESVAKDREMEGDILCRNI---- 2191
              NSQ +                   L+  +   +Q+     +D ++    + RNI    
Sbjct: 1172 SGNSQSKSLEIITHLDNLQMLLKDGGLISKVNEFLQRKFKSLRDVDVIARDITRNIGENG 1231

Query: 2192 -----------------AMLFEACTSVIKEVDERKGELMGHDLTSGNL-----GMEIIST 2251
                             ++L +   SV  E +  +G     D  S +L     G+ + + 
Sbjct: 1232 LLAGEMGNAEDDSTEAKSLLSDLDNSVNTEPENSQGSAADEDEISSSLRKMAEGVRLRNK 1291

Query: 2252 TPDQLSHAGKTHL------LSEESVRKIADRL-LLAVREFIGFKAEMLDGSLKEMKVSVA 2311
            T +       T +      L +      AD L ++     +  +   ++  ++E + +++
Sbjct: 1292 TLENNFEGFSTSIDTLIATLMQNMTAARADVLNIVGHNSSLEEQVRSVENIVREQENTIS 1351

Query: 2312 NLQKEL-----------QEKDIQKERICMELVGQIK-----EAEATASRYSIDLQASKDQ 2371
             LQK+L           +E  ++ +   +ELV   +     E E+T     + +     +
Sbjct: 1352 ALQKDLSSLISACGAAARELQLEVKNNLLELVQFQENENGGEMESTEDPQELHVSECAQR 1411

Query: 2372 VYELEKVTEQMDSERKVLEQR-------LREMQDGLSISDELRERVRLLTGSLAAKDQEI 2431
            + EL    E+  +  K+ E         +R+M++ L+ +    E+  +       K+ E+
Sbjct: 1412 IKELSSAAEKACATLKLFETTNNAAATVIRDMENRLTEASVALEKAVVKEEKWHEKEVEL 1471

Query: 2432 EALMHALDEEELQMEGLTKKIEDLERDLKQKNQ-ELESIEASRG------KLMKKLSITV 2491
              L   L  +E + +       D+     + N  E+ S++   G        +KKL   V
Sbjct: 1472 STLYDKLLVQEQEAKENLIPASDMRTLFDKINGIEVPSVDLVNGLDPQSPYDVKKLFAIV 1531

Query: 2492 TKFDELHHLSESLLTEVEKLQAQLQDRDAEISFLRQEVTRCTNDALVATQTSNRNSEDIN 2551
                E+ H  + L    ++L + L ++D EI  L++     +   L   +  N    +++
Sbjct: 1532 DSVTEMQHQIDILSYGQKELNSTLAEKDLEIQGLKKATEAESTTELELVKAKN----ELS 1591

Query: 2552 EVITWFDTMEARVGLSHSGHDDKENEVRECKEVLKKKITSILKGIEDLQAESQR------ 2611
            ++I+  + +   +  ++   D   +E     + L+KKITS+L   E  ++ +Q       
Sbjct: 1592 KLISGLEKLLGILASNNPVVDPNFSESWTLVQALEKKITSLLLESESSKSRAQELGLKLA 1651

Query: 2612 -----KDEMLLAEKNKVEELKRKELQLNLLED--VGDNNRASSVAPEIFESEPLHIVKSC 2636
                  D++ L  K   E+L+ K +Q +++++  + +  RA S + EI E E        
Sbjct: 1652 GSEKLVDKLSLRVKEFEEKLQTKAIQPDIVQERSIFETPRAPSTS-EISEIE-------- 1711

BLAST of MC11g0525 vs. TAIR 10
Match: AT1G24460.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G31570.1); Has 181008 Blast hits to 85359 proteins in 3551 species: Archae - 3290; Bacteria - 48304; Metazoa - 70793; Fungi - 13943; Plants - 10118; Viruses - 785; Other Eukaryotes - 33775 (source: NCBI BLink). )

HSP 1 Score: 146.0 bits (367), Expect = 4.4e-34
Identity = 360/1689 (21.31%), Postives = 681/1689 (40.32%), Query Frame = 0

Query: 1172 YLESMVSLLLQKYKE-----TELQ-------LGLSREKS-----GSLMMKLTELQESVHD 1231
            +LE+ VS L  KY E      +L+       L LS ++      G+   +L EL++    
Sbjct: 212  HLENRVSFLGAKYTEFYYGADQLRKCLASDVLDLSFQEDFGSALGAACSELFELKQKEAA 271

Query: 1232 LSTLILDHECEIVILKESLSQVQEALVASRSVLKDKANELEQSEQRVSAIREKLSIAVAK 1291
                +   E E     E +++ +E   + R+  +    ELE  + + +  +EKLS+AV K
Sbjct: 272  FFERLSHLEDENRNFVEQVNREKEMCESMRTEFEKLKAELELEKTKCTNTKEKLSMAVTK 331

Query: 1292 GKGLIVQRDNLKQSLAQTSSELERCLQELQMKDTRLHETET-KLTAYSEAGERVEALESE 1351
            GK L+  RD LK  L++ ++EL   L ELQ K+  L  +E  K        E+ + LE  
Sbjct: 332  GKALVQNRDALKHQLSEKTTELANRLTELQEKEIALESSEVMKGQLEQSLTEKTDELEKC 391

Query: 1352 LSYIRNSATALRESFLLKDSVLQRIEEILDELDLPENFHSRDIIEKVDWLAKSSTGENLP 1411
             + + + + +L    L K  + Q + E   EL+           E +  L + ST  +  
Sbjct: 392  YAELNDRSVSLEAYELTKKELEQSLAEKTKELE-----------ECLTKLQEMSTALDQS 451

Query: 1412 HTDWDQRSSVAGGSGSDANFVIMDAWKDEVQMDANVGDDLRRKYEELQTRFYGLAEQNEM 1471
              D   +  +A      A++  M + ++ +    N+   L   Y   +   + + E+   
Sbjct: 452  ELD---KGELAKSDAMVASYQEMLSVRNSI--IENIETILSNIYTPEEGHSFDIVEKVRS 511

Query: 1472 LEQSLMERNNIVQ---RWEELLEKIDIPSQLRSMEPEDKMEWLHKSLSEAFHDRDSLHQR 1531
            L +   E  N+ Q   R ++L+  ID+P ++     E ++ WL +S  +    +D ++  
Sbjct: 512  LAEERKELTNVSQEYNRLKDLIVSIDLPEEMSQSSLESRLAWLRESFLQG---KDEVNAL 571

Query: 1532 VNYLENYCGLLTADLDDSQKKISDLEAELHSLVLEREKLSEKLEIIYHHNENLAFGNFEN 1591
             N +E+    L+A++++     S++  EL  L    +K+ E  E           G+ E 
Sbjct: 572  QNRIESVSMSLSAEMEEK----SNIRKELDDLSFSLKKMEETAE----------RGSLER 631

Query: 1592 EVGIIVLQNELSNMQENLISTEHKIVKLEALVSNALQD---EDVNDLVSGSSIEFLDLMV 1651
            E                      ++V+   L++  ++D    D+N LV  S  +     +
Sbjct: 632  E------------------EIVRRLVETSGLMTEGVEDHTSSDINLLVDRSFDK-----I 691

Query: 1652 TKLIQNYTASSSGKAVLGKATNG----HDTEEEMLARSKNMHDAWQNDINILKKELEDAL 1711
             K I++ + SS G   + +A        D E  +        +     ++ L  EL+ A 
Sbjct: 692  EKQIRDSSDSSYGNEEIFEAFQSLLYVRDLEFSLCKEMLGEGELISFQVSNLSDELKIAS 751

Query: 1712 NQLMVVTEERDQYMEMHESLVVKVESSDKKKDELQELLNLEEQKSASIREKLNVAVRKGK 1771
             +L  V EE                     K  L++ L   E+KSA +R+KL++A++KGK
Sbjct: 752  QELAFVKEE---------------------KIALEKDLERSEEKSALLRDKLSMAIKKGK 811

Query: 1772 SLIQQRDSLKQAVEEMTTELKHLRSEMKSQENTLASYEQNFKDFSVYSGRAEALESENLS 1831
             L+Q R+  K  ++E  +E++ L  E++    T+  Y+      S    R + LE+E ++
Sbjct: 812  GLVQDREKFKTQLDEKKSEIEKLMLELQQLGGTVDGYKNQIDMLSRDLERTKELETELVA 871

Query: 1832 LKNRLAEIERNFQEKEHKLSSIINTLVHIEDNVDVSENDPIEKLKQVGKLCSDLREAMYF 1891
             K    +++++    +  L  ++ ++  I   VD++  DP EK+ ++     +++ A   
Sbjct: 872  TKEERDQLQQSLSLIDTLLQKVMKSVEIIALPVDLASEDPSEKIDRLAGYIQEVQLARVE 931

Query: 1892 SEQESVKSRRAAELLLAELNEVQERNDTFQEELAKASDEIAELTKERDLAETSKLEALSE 1951
             ++E  K +   + L ++L E Q      ++ L+ A D I+ LT+E    + +K  A  E
Sbjct: 932  EQEEIEKVKSEVDALTSKLAETQTALKLVEDALSTAEDNISRLTEENRNVQAAKENAELE 991

Query: 1952 LEKLSTLHSKEKKIQYSQFMGLKSGLD-RLKEAMREINCLLAD--------AFSKDLDAF 2011
            L+K +   +     +  + +  KS L+  L +A R I+ ++++        A ++     
Sbjct: 992  LQK-AVADASSVASELDEVLATKSTLEAALMQAERNISDIISEKEEAQGRTATAEMEQEM 1051

Query: 2012 YNLEAAIQSCTEPNGPADVNLSHSIVSGAFKSRDL------KDKGKFFSL-DSWSNFYTN 2071
               EA+IQ        + +N     ++    + D        DK    SL +        
Sbjct: 1052 LQKEASIQKNKLTEAHSTINSLEETLAQTESNMDSLSKQIEDDKVLTTSLKNELEKLKIE 1111

Query: 2072 AHVDENVATEIHSLVHQLEESMKEIGALKEMIDGHSVSFHKQSDSLSKILGVLYREV--- 2131
            A  + N   E    +   EE++ +       + G  V    +  +LS  L V   E+   
Sbjct: 1112 AEFERNKMAEASLTIVSHEEALMKAENSLSALQGEMVKAEGEISTLSSKLNVCMEELAGS 1171

Query: 2132 --NSQKELVQALKWDVQQSESVAKDREMEGDI---------LCRNIAMLFEACTSVIKEV 2191
              NSQ + ++ +   +   + + KD  +   +           R++ ++    T  I E 
Sbjct: 1172 SGNSQSKSLEIIT-HLDNLQMLLKDGGLISKVNEFLQRKFKSLRDVDVIARDITRNIGEN 1231

Query: 2192 DERKGELMGHDLTSGNLGMEI-------------------ISTTPDQLSHAGKTHLLSEE 2251
                GE+   ++T+  L   +                   ++T P+    +         
Sbjct: 1232 GLLAGEMGNAEVTAVLLITLLYFQDDSTEAKSLLSDLDNSVNTEPENSQGSAADEDEISS 1291

Query: 2252 SVRKIADRLLLAVR----EFIGF------------------KAEML-----DGSLKEMKV 2311
            S+RK+A+ + L  +     F GF                  +A++L     + SL+E   
Sbjct: 1292 SLRKMAEGVRLRNKTLENNFEGFSTSIDTLIATLMQNMTAARADVLNIVGHNSSLEEQVR 1351

Query: 2312 SVAN-----------LQKEL-----------QEKDIQKERICMELVGQIK-----EAEAT 2371
            SV N           LQK+L           +E  ++ +   +ELV   +     E E+T
Sbjct: 1352 SVENIVREQENTISALQKDLSSLISACGAAARELQLEVKNNLLELVQFQENENGGEMEST 1411

Query: 2372 ASRYSIDLQASKDQVYELEKVTEQMDSERKVLEQR-------LREMQDGLSISDELRERV 2431
                 + +     ++ EL    E+  +  K+ E         +R+M++ L+ +    E+ 
Sbjct: 1412 EDPQELHVSECAQRIKELSSAAEKACATLKLFETTNNAAATVIRDMENRLTEASVALEKA 1471

Query: 2432 RL-------LTGSLAAKDQEIEALMHAL---------DEEELQMEGLTKKIEDLER---- 2491
             L          S  AK + +E L   L          E+E+++  L  K+   E+    
Sbjct: 1472 VLERDLNQTKVSSSEAKVESLEELCQDLKLQVKEEKWHEKEVELSTLYDKLLVQEQGNFY 1531

Query: 2492 --------------------------------------DLKQKNQELESIEASRGKL--- 2551
                                                  D++    ++  IE     L   
Sbjct: 1532 LLLSLISLNLHHIITTILKCHVLLLRIAEAKENLIPASDMRTLFDKINGIEVPSVDLVNG 1591

Query: 2552 --------MKKLSITVTKFDELHHLSESLLTEVEKLQAQLQDRDAEISFLRQEVTRCTND 2611
                    +KKL   V    E+ H  + L    ++L + L ++D EI  L++     +  
Sbjct: 1592 LDPQSPYDVKKLFAIVDSVTEMQHQIDILSYGQKELNSTLAEKDLEIQGLKKATEAESTT 1651

Query: 2612 ALVATQTSNRNSEDINEVITWFDTMEARVGLSHSGHDDKENEVRECKEVLKKKITSILKG 2636
             L   +  N    +++++I+  + +   +  ++   D   +E     + L+KKITS+L  
Sbjct: 1652 ELELVKAKN----ELSKLISGLEKLLGILASNNPVVDPNFSESWTLVQALEKKITSLLLE 1711

BLAST of MC11g0525 vs. TAIR 10
Match: AT5G41790.1 (COP1-interactive protein 1 )

HSP 1 Score: 91.3 bits (225), Expect = 1.3e-17
Identity = 263/1284 (20.48%), Postives = 535/1284 (41.67%), Query Frame = 0

Query: 1284 NLKQSLAQTSSELERCLQELQMKDTRLHETETKLTAYSEAGERVEALES-ELSYIRNSAT 1343
            +LK  L  T  E E    EL++   +L E+E   +      E++E  +S  LS  R    
Sbjct: 144  DLKGKLTTTVEEKEAVDSELELALMKLKESEEISSKLKLETEKLEDEKSIALSDNRELHQ 203

Query: 1344 ALRESFLLKDSVLQRIEEILDELDLPENFHSRDIIEKVDWLAKSSTGENLPHTDWDQRSS 1403
             L  +   +  + Q++E+I  E D  +        E+ + + +    E +   DW   S 
Sbjct: 204  KLEVAGKTETDLNQKLEDIKKERDELQT-------ERDNGIKRFQEAEKVAE-DWKTTS- 263

Query: 1404 VAGGSGSDANFVIMDAWKDEVQMDANVGDDLRRKYEELQTRFYGLAEQNEMLEQSLMERN 1463
                          D  KDE        +   ++  EL +      E+N+ L   + E +
Sbjct: 264  --------------DQLKDETSNLKQQLEASEQRVSELTSGMNSAEEENKSLSLKVSEIS 323

Query: 1464 NIVQRWEELLEKIDIPSQLRSMEPEDK-MEWLHKSLSEAF--HDRDSLHQRVNYLENYCG 1523
            +++Q+ +  ++  ++ S+L  M+ + K  E  H SL E    H+R+S  Q V  LE    
Sbjct: 324  DVIQQGQTTIQ--ELISELGEMKEKYKEKESEHSSLVELHKTHERESSSQ-VKELE---- 383

Query: 1524 LLTADLDDSQKKISDLEAELHSLVLEREKLSEKL-----EIIYHHN--ENLAFGNFENEV 1583
               A ++ S+K ++D    L++   E++ LS+K+     EI    N  + L   + + + 
Sbjct: 384  ---AHIESSEKLVADFTQSLNNAEEEKKLLSQKIAELSNEIQEAQNTMQELMSESGQLKE 443

Query: 1584 GIIVLQNEL------------------SNMQENLISTEHKIVKLEALVSNALQDEDVNDL 1643
               V + EL                  S ++  L S++ ++  L A +  A   E+ N  
Sbjct: 444  SHSVKERELFSLRDIHEIHQRDSSTRASELEAQLESSKQQVSDLSASLKAA---EEENKA 503

Query: 1644 VSGSSIEFLDLMVTKLIQNYTASSSGKAVLGKATNGHDTEEEMLARSKNMHDAWQNDINI 1703
            +S  ++E ++    KL Q         A LGK  + H  +E  L+    +H+  Q D +I
Sbjct: 504  ISSKNVETMN----KLEQTQNTIQELMAELGKLKDSHREKESELSSLVEVHETHQRDSSI 563

Query: 1704 LKKELEDALNQLMVVTEERDQYM----EMHESLVVKVESSDKKKDELQELLNLEEQKSAS 1763
              KELE+ +     +  E +Q +    E  + L  K+     +  E Q  +     +S  
Sbjct: 564  HVKELEEQVESSKKLVAELNQTLNNAEEEKKVLSQKIAELSNEIKEAQNTIQELVSESGQ 623

Query: 1764 IREKLNVAVRKGKSLIQQRDSLKQAVEEMTTELKHLRSEMKSQENTLASYEQNFKDFSVY 1823
            ++E  +V   K + L   RD  +    E +T +  L ++++S E  ++    + KD    
Sbjct: 624  LKESHSV---KDRDLFSLRDIHETHQRESSTRVSELEAQLESSEQRISDLTVDLKD---- 683

Query: 1824 SGRAEALESENLSLKNRLAEIERNFQEKEHKLSSIINTLVHIEDNVDVSENDPIEKLKQV 1883
                   E EN ++ ++  EI    ++ ++ +  +++ L  ++D     E++    +K  
Sbjct: 684  ------AEEENKAISSKNLEIMDKLEQAQNTIKELMDELGELKDRHKEKESELSSLVKSA 743

Query: 1884 GKLCSDLREAMYFSEQESVKSRRAAELLLAELNEVQERNDTFQEELAKASD-------EI 1943
             +  +D+++++  +E+E    +  ++ +L   NE+QE   T QE ++++         + 
Sbjct: 744  DQQVADMKQSLDNAEEE---KKMLSQRILDISNEIQEAQKTIQEHMSESEQLKESHGVKE 803

Query: 1944 AELTKERDLAETSKLEALSELEKLST-----------------LHSKEKKIQYSQFMGLK 2003
             ELT  RD+ ET + E+ + L +L T                    +EKK   S  + + 
Sbjct: 804  RELTGLRDIHETHQRESSTRLSELETQLKLLEQRVVDLSASLNAAEEEKKSLSSMILEI- 863

Query: 2004 SGLDRLKEAMREINCLLADAFSKDLDAFYNLEAAIQSCTEPNGPADVNLSHSIVSGAFKS 2063
               D LK+A  ++  L+ +  ++  D     E  + S  E +     + S  +     + 
Sbjct: 864  --TDELKQAQSKVQELVTE-LAESKDTLTQKENELSSFVEVHEAHKRDSSSQVKELEARV 923

Query: 2064 RDLKDKGKFFSLDSWSNFYTNAHVDENVATEIHSLVHQLEESMKEIGALKEMIDGHSVSF 2123
               +++ K  + +  S+      + + + +E+   + + E +++E+ +  E + G     
Sbjct: 924  ESAEEQVKELNQNLNSSEEEKKILSQQI-SEMSIKIKRAESTIQELSSESERLKGSHAEK 983

Query: 2124 HKQSDSLSKILGVLYREVNSQKELVQALKWDVQQSESVAKDREMEGDILCRNIAMLFEAC 2183
              +  SL  I     RE+++Q   ++ L+  ++ SE     R +E   L  ++    E  
Sbjct: 984  DNELFSLRDIHETHQRELSTQ---LRGLEAQLESSE----HRVLE---LSESLKAAEEES 1043

Query: 2184 TSVIKEVDERKGELMGHDLTSGNLGMEIISTTPDQLSHAGKTHLLSE-ESVRKIADRLLL 2243
             ++  ++ E   EL    +    L  +             K  LL+E +S  ++  + L 
Sbjct: 1044 RTMSTKISETSDELERTQIMVQELTADSSKLKEQLAEKESKLFLLTEKDSKSQVQIKELE 1103

Query: 2244 AVREFIGFKAEMLDGSLKEMKVSVANLQKELQEKDIQKERICMELVGQIKEAEATASRYS 2303
            A    +  + E +   + +++  +A+    +++ + Q      E+V +I E E T     
Sbjct: 1104 ATVATLELELESVRARIIDLETEIASKTTVVEQLEAQNR----EMVARISELEKTMEERG 1163

Query: 2304 IDLQASKDQVYELEKVTEQMDSERKVLEQRLREMQDGLSISDELRERVRLLTGSLAAKDQ 2363
             +L A   ++ + +K +             LR   D +S+  E  E+       +  K +
Sbjct: 1164 TELSALTQKLEDNDKQSSSSIETLTAEIDGLRAELDSMSVQKEEVEK------QMVCKSE 1223

Query: 2364 EIEALMHALDEE----ELQMEGLTKKIEDLERDLKQKNQE----LESIEASRGKLMKKLS 2423
            E    +  LD+E      Q+  L  +  +LE  L++K++E    L  I   + +++ K+ 
Sbjct: 1224 EASVKIKRLDDEVNGLRQQVASLDSQRAELEIQLEKKSEEISEYLSQITNLKEEIINKVK 1283

Query: 2424 ITVTKFDELHHLSESLL---TEVEKLQAQLQDRDAEISFLRQEVTRCTNDALVATQTSNR 2483
            +  +  +E++ LSE +     E+E L  Q  + D E+   ++E  +  +   VA+     
Sbjct: 1284 VHESILEEINGLSEKIKGRELELETLGKQRSELDEELRTKKEENVQMHDKINVASSEIMA 1338

Query: 2484 NSEDINEVITWFDTMEARVGLSHSGHDDKENEVRECKEVLKKKITSILKGIEDLQAESQR 2498
             +E IN +    D+++ +   + +   + E E +E K  L  +IT + K + + +A    
Sbjct: 1344 LTELINNLKNELDSLQVQKSETEA---ELEREKQE-KSELSNQITDVQKALVEQEAAYNT 1338

BLAST of MC11g0525 vs. TAIR 10
Match: AT2G32240.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response to cadmium ion; LOCATED IN: plasma membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Prefoldin (InterPro:IPR009053); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G05320.3); Has 470429 Blast hits to 168274 proteins in 4282 species: Archae - 6896; Bacteria - 131956; Metazoa - 175525; Fungi - 33166; Plants - 25441; Viruses - 2243; Other Eukaryotes - 95202 (source: NCBI BLink). )

HSP 1 Score: 54.7 bits (130), Expect = 1.3e-06
Identity = 223/1178 (18.93%), Postives = 483/1178 (41.00%), Query Frame = 0

Query: 1369 ENFHSRDIIEKVDWLAKSSTGENLPHTDWDQRSSVAGGSGSDANFVIMDAWKDEVQMDAN 1428
            E F ++D  EK D +      E +      +RSS   GS  + +     A + E++++  
Sbjct: 54   EAFDAKDDAEKADHVPVEEQKEVI------ERSS--SGSQRELHESQEKAKELELELERV 113

Query: 1429 VGDDLRRKYEELQTRFYGLAEQNEMLEQSLMERNNIVQRWEELLEKIDIPSQLRSMEPED 1488
             G+   ++YE   T       ++E+L  S  E+    ++    LE +    Q + +E E+
Sbjct: 114  AGE--LKRYESENTHL-----KDELL--SAKEKLEETEKKHGDLEVVQKKQQEKIVEGEE 173

Query: 1489 KMEWLHKSLSEAFHDRDSLHQRVNYLENYCGLLTADLDDSQKKISDLEAELHSLVLEREK 1548
            +     KSL +A    D+  + +  ++     L  +L+ S+KK+ +LE  L     E +K
Sbjct: 174  RHSSQLKSLEDALQSHDAKDKELTEVKEAFDALGIELESSRKKLIELEEGLKRSAEEAQK 233

Query: 1549 LSEKLEIIYHHNENLAFGNFENEVGIIVLQNELSNMQENLISTEHKIVKLEALVSNALQD 1608
              E  +    H ++ +    E    +   +     M+E + S + +I +L   +S   ++
Sbjct: 234  FEELHKQSASHADSESQKALEFSELLKSTKESAKEMEEKMASLQQEIKELNEKMS---EN 293

Query: 1609 EDVNDLVSGSSIEF------LDLMVTKLIQNYTASSSGKAVLGKATNGHDTEEEMLARSK 1668
            E V   +  S+ E       L L  ++L++     SS +A++ + T   + E++  + S+
Sbjct: 294  EKVEAALKSSAGELAAVQEELALSKSRLLETEQKVSSTEALIDELT--QELEQKKASESR 353

Query: 1669 NMHDAWQNDINILKKELEDALNQLMVVTEERDQYMEMHESLVVKVESSDKKKDELQELLN 1728
                         K+EL   L  L   T+     +   E +  K+    K+K+ L+ L  
Sbjct: 354  ------------FKEEL-SVLQDLDAQTKGLQAKLSEQEGINSKLAEELKEKELLESLSK 413

Query: 1729 LEEQKSASIREKLNVAVRKGKSLIQQRDSLKQAVEEMTTELKHLRSEMKSQENTLASYEQ 1788
             +E+K  +  EKL         +++++++L+  V E+T+ +  +       E  L + ++
Sbjct: 414  DQEEKLRTANEKL-------AEVLKEKEALEANVAEVTSNVATVTEVCNELEEKLKTSDE 473

Query: 1789 NFKDFSVYSGRAEALESENLSLKNRLAEIERNFQEKEHKLSSIINTLVHIEDNVDVSEND 1848
            NF        +A    S N  L+ +L  +E    E     ++     + +ED V  S   
Sbjct: 474  NFSKTDALLSQA---LSNNSELEQKLKSLEELHSEAGSAAAAATQKNLELEDVVRSSSQA 533

Query: 1849 PIEKLKQVGKLCSDLREAMYFSEQESVKSRRAAELLLAELNEVQERNDTFQEELAKASDE 1908
              E   Q+     +L      +EQ++ +       L  +LN +Q ++   + EL + S++
Sbjct: 534  AEEAKSQI----KELETKFTAAEQKNAE-------LEQQLNLLQLKSSDAERELKELSEK 593

Query: 1909 IAELTKERDLAETSKLEALSELEKLSTLHSKEKKIQYSQFMGLKSGLDRLKEAMREINCL 1968
             +EL    ++AE  K +A +++++                          K+   E+   
Sbjct: 594  SSELQTAIEVAEEEKKQATTQMQE-------------------------YKQKASELELS 653

Query: 1969 LADAFSKDLDAFYNLEAAIQSCTEPNGPADVNLSHSI------VSGAFKSRDLKDKGKFF 2028
            L  + +++ +   +L  A+Q   E    A+     SI       S   K  D + + K  
Sbjct: 654  LTQSSARNSELEEDLRIALQKGAEHEDRANTTHQRSIELEGLCQSSQSKHEDAEGRLKDL 713

Query: 2029 SLDSWSNFYTNAHVDENVATEIHSLVHQLEESMKEIGALKEMIDGHSVSFHKQSDSLSKI 2088
             L   +  Y    ++E V++          +S   +G + E +     +F  +S SL   
Sbjct: 714  ELLLQTEKYRIQELEEQVSSLEKKHGETEADSKGYLGQVAE-LQSTLEAFQVKSSSLEAA 773

Query: 2089 LGVLYREVNSQKELVQALKWDVQQSESVAKDREMEGDILCRNIAMLFEACTSVIKEVDER 2148
            L +      ++KEL +    ++    S  K  E   D     I+       S+  E++  
Sbjct: 774  LNI---ATENEKELTE----NLNAVTSEKKKLEATVDEYSVKISESENLLESIRNELNVT 833

Query: 2149 KGEL--MGHDLTSGNLG----MEIISTTPDQLSHAGK------THLLSEESVRKIAD--- 2208
            +G+L  + +DL +  L     ME + +  + L   G+      T  +  E++ +      
Sbjct: 834  QGKLESIENDLKAAGLQESEVMEKLKSAEESLEQKGREIDEATTKRMELEALHQSLSIDS 893

Query: 2209 --RLLLAVREFIG--FKAEMLDGSLKEMKVSVANLQKELQE---KDIQKERICMELVGQI 2268
              RL  A+ EF     +A  L   L++++  + + +++L E   K    +    + +G++
Sbjct: 894  EHRLQKAMEEFTSRDSEASSLTEKLRDLEGKIKSYEEQLAEASGKSSSLKEKLEQTLGRL 953

Query: 2269 KEAEATASRYSIDLQASKDQVYELEKVTEQMDSERKVLEQRLREMQ----DGLSISDELR 2328
              AE+   +   +   ++++  +    +E +      L+ +++E++     G    +   
Sbjct: 954  AAAESVNEKLKQEFDQAQEKSLQSSSESELLAETNNQLKIKIQELEGLIGSGSVEKETAL 1013

Query: 2329 ERVRLLTGSLAAKDQEIEALMHALDEEELQMEGLTKKIEDL-------ERDLKQKNQELE 2388
            +R+         K+ E   L+  L   E Q+E   K   +        + +L+    +L+
Sbjct: 1014 KRLEEAIERFNQKETESSDLVEKLKTHENQIEEYKKLAHEASGVADTRKVELEDALSKLK 1073

Query: 2389 SIEASRGKLMKKLSITVTKFDELHHLSESLLTEVEKLQAQLQDRDAEISFLRQEVTRCTN 2448
            ++E++  +L  K      +  +L  ++  L  E+    ++  +   ++S L  E  +  N
Sbjct: 1074 NLESTIEELGAKCQGLEKESGDLAEVNLKLNLELANHGSEANELQTKLSALEAEKEQTAN 1131

Query: 2449 DALVATQTSNRNSEDINEVITWFDTMEARVGLSHSGHDDKENEVRECKEVLKKKITSILK 2501
            +     + S    ED+ + +T   +   ++    S H ++ N+V    +  K+++ S++ 
Sbjct: 1134 E----LEASKTTIEDLTKQLT---SEGEKLQSQISSHTEENNQVNAMFQSTKEELQSVIA 1131