MC11g0497 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g0497 vs. ExPASy Swiss-Prot
Match: Q9M084 (Aspartate--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=IBI1 PE=1 SV=1)

HSP 1 Score: 731.5 bits (1887), Expect = 7.0e-210
Identity = 379/558 (67.92%), Postives = 440/558 (78.85%), Query Frame = 0

Query: 1   MSAPSETPIEEPPSAGA------VSKKAAKKEAAKQEKLRRRQE-AAAEAAISSLNVEE- 60
           MS+ SE P     +A A       SKKAAKKEAAK EKLRRRQE   A    +S+++EE 
Sbjct: 1   MSSESEIPPLSSSTAAAEESGEKTSKKAAKKEAAKLEKLRRRQEQEEATRRTASISLEEN 60

Query: 61  DPLSANYGDVPLSDLQSK---------EAKNIENWTQVGSLTEVLKDKYVLLRGRIQTIR 120
           D  S NYGDV L++LQS          EA   + WT V  L E + +  VL+RGR+ T R
Sbjct: 61  DEFSNNYGDVTLTELQSSADPKAGKWIEAVEGKEWTDVSDLVEEMLESEVLIRGRVHTNR 120

Query: 121 AVAKKMAFLVMREKGFTVQCVVSEQPEV-VSRQMVKYVVGLSRESIVDVEGVVSVPNVAI 180
             + K+ F+V+RE G TVQCVVS+  +  V   MVKY+  LSRES VDV GVV++P   +
Sbjct: 121 PTSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSRESFVDVIGVVTLPKEPL 180

Query: 181 KGATQQVEIQVRKVYCISKAMPSLPINIEDAARSEVEIEKALQAGEQLVRVNQDTRLNYR 240
            G TQQVEIQVRKVYCI+K++  LP+++EDAARSE +IE +LQ      RVNQDTRLNYR
Sbjct: 181 TGTTQQVEIQVRKVYCINKSLAKLPLSVEDAARSEADIEASLQTPSPAARVNQDTRLNYR 240

Query: 241 VLDIRTPANQGIFRIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFKLDYKGQP 300
           VLD+RTPANQ IF++Q +V   FR+ L  + FV IHTPKL+ GSSEGG++VF+L+YKGQP
Sbjct: 241 VLDLRTPANQAIFQLQYEVEYAFREKLRFKNFVGIHTPKLMAGSSEGGSAVFRLEYKGQP 300

Query: 301 ACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMELKTHYSEVM 360
           ACLAQSPQLHKQMAICGD  RVFE+GPVFRAEDSFTHRHLCEF GLDVEME++ HYSE+M
Sbjct: 301 ACLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRKHYSEIM 360

Query: 361 DIVDRLFVSMFDSLNQNCKKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQMLKDAGVQID 420
           D+VD LFV +F SLN+ CKKEL+AV +QYPFEPLK+L  TLRLTFEEGVQMLK+AGV++D
Sbjct: 361 DLVDELFVFIFTSLNERCKKELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEVD 420

Query: 421 PLGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCHDNPTYSNSFDVFIRGE 480
           PLGDLNTE+ERKLGQLVLEKY TEFYILHRYP AVRPFYTM C DNP YSNSFDVFIRGE
Sbjct: 421 PLGDLNTESERKLGQLVLEKYNTEFYILHRYPKAVRPFYTMTCADNPLYSNSFDVFIRGE 480

Query: 481 EIISGAQRVHVPEFLAERAQACGIEVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGL 540
           EIISGAQRVH+PE L +RA  CGI+VKTI TYIDSFRYGAP HGGFGVGLERVVMLFC L
Sbjct: 481 EIISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCAL 540

BLAST of MC11g0497 vs. ExPASy Swiss-Prot
Match: Q8H104 (Aspartate--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At4g26870 PE=2 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 1.2e-196
Identity = 351/526 (66.73%), Postives = 421/526 (80.04%), Query Frame = 0

Query: 18  VSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEE--DPLSANYGDVPLSDLQSK-EAKN 77
           +SKK +KK AAK EKL R+QE   EA  SSL++EE  +  S+NYGDV  ++LQS  E K 
Sbjct: 14  ISKKESKKRAAKLEKLLRKQE-REEATSSSLSLEEEDESCSSNYGDVTTNELQSAVEGKE 73

Query: 78  IENWTQVGSLTEVLKDKYVLLRGRIQTIRAVAKKMAFLVMREKGFTVQCVVSEQPEVVSR 137
           +   T V +L E +    V +RGR+   R V  K+ F+++RE GFTVQCVV E    V  
Sbjct: 74  L---TDVSNLVEEIVGSEVSIRGRLHKNRLVGTKL-FVILRESGFTVQCVVEETR--VGA 133

Query: 138 QMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCISKAMPSLPINIEDAA 197
            M+K+V  LSRES+V++ GVVS P   + G TQQVEI VRK+YC+S+++P+LP+ +EDAA
Sbjct: 134 NMIKFVKQLSRESVVELIGVVSHPKKPLTGTTQQVEIHVRKMYCLSRSLPNLPLVVEDAA 193

Query: 198 RSEVEIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIFRIQCQVGTIFRQFLLSEGF 257
           RSE +IEK+ + G+Q  RV QDTRLN RVLDIRTPANQ IFRIQCQV   FR++L S+GF
Sbjct: 194 RSESDIEKSGKDGKQAARVLQDTRLNNRVLDIRTPANQAIFRIQCQVQIAFREYLQSKGF 253

Query: 258 VEIHTPKLIGGSSEGGASVFKLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGPVFRAE 317
           +EIHTPKLI GSSEGG++VF+LDYKGQPACLAQSPQLHKQMAICGD  RVFE+GPVFRAE
Sbjct: 254 LEIHTPKLIAGSSEGGSAVFRLDYKGQPACLAQSPQLHKQMAICGDMRRVFEVGPVFRAE 313

Query: 318 DSFTHRHLCEFTGLDVEMELKTHYSEVMDIVDRLFVSMFDSLNQNCKKELEAVDRQYPFE 377
           DSFTHRHLCEF GLDVEME++ HYSE+MD+V  LF  +F  + + C KELE+V +QYPF+
Sbjct: 314 DSFTHRHLCEFVGLDVEMEIRMHYSEIMDLVGELFPFIFTKIEERCPKELESVRKQYPFQ 373

Query: 378 PLKYLRNTLRLTFEEGVQMLKDAGVQIDPLGDLNTEAERKLGQLVLEKYGTEFYILHRYP 437
            LK+L  TLRLTF EG+QMLK+AG ++DPLGDLNTE+ERKLGQLVLEKY TEFY+LHRYP
Sbjct: 374 SLKFLPQTLRLTFAEGIQMLKEAGEEVDPLGDLNTESERKLGQLVLEKYKTEFYMLHRYP 433

Query: 438 LAVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIEVKTIETY 497
            AVRPFYTMP  ++  YSNSFDVFIRGEEI+SGAQR+H PE L +RA+ CGI+VKTI TY
Sbjct: 434 SAVRPFYTMPYENDSNYSNSFDVFIRGEEIMSGAQRIHDPELLEKRARECGIDVKTISTY 493

Query: 498 IDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 541
           ID+FRYGAPPHGGFGVGLERVVML C LNNIRKTSLFPRD QR+ P
Sbjct: 494 IDAFRYGAPPHGGFGVGLERVVMLLCALNNIRKTSLFPRDSQRLTP 532

BLAST of MC11g0497 vs. ExPASy Swiss-Prot
Match: Q5R9I5 (Aspartate--tRNA ligase, cytoplasmic OS=Pongo abelii OX=9601 GN=DARS1 PE=2 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 1.4e-133
Identity = 255/508 (50.20%), Postives = 345/508 (67.91%), Query Frame = 0

Query: 34  RRRQEAAAEAAISSLNVEEDPLSANYGDVPLSDLQSKEAKNIENWTQVGSLTEVLKDKYV 93
           R+ QE   E     ++  ED     YG   +S +   + K+     +V  LT    D+ V
Sbjct: 8   RKSQEKPREI----MDAAEDYAKERYG---ISSMIQSQEKSDRVLVRVRDLTIQKADEVV 67

Query: 94  LLRGRIQTIRAVAKKMAFLVMREKGFTVQCVVSEQPEVVSRQMVKYVVGLSRESIVDVEG 153
            +R R+ T RA  K+  FLV+R++ F VQ +V+   +  S+QMVK+   +++ESIVDVEG
Sbjct: 68  WVRARVHTSRAKGKQ-CFLVLRQQQFNVQALVA-VGDHASKQMVKFAANINKESIVDVEG 127

Query: 154 VVSVPNVAIKGATQQ-VEIQVRKVYCISKAMPSLPINIEDAARSEVEIEKALQAGEQLVR 213
           VV   N  I+  TQQ VE+ V+K+Y IS A P LP+ ++DA R E E E+     E    
Sbjct: 128 VVRKVNQKIESCTQQDVELHVQKIYVISLAEPRLPLQLDDAVRPEAEGEE-----EGRAT 187

Query: 214 VNQDTRLNYRVLDIRTPANQGIFRIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGAS 273
           VNQDTRL+ RV+D+RT  +Q +FR+Q  +  +FR+ L+++GFVEI TPK+I  +SEGGA+
Sbjct: 188 VNQDTRLDNRVIDLRTSTSQAVFRLQSGICHLFRETLINKGFVEIQTPKIISAASEGGAN 247

Query: 274 VFKLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEM 333
           VF + Y    A LAQSPQL+KQM IC DF +VF IGPVFRAEDS THRHL EF GLD+EM
Sbjct: 248 VFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFCIGPVFRAEDSNTHRHLTEFVGLDIEM 307

Query: 334 ELKTHYSEVMDIVDRLFVSMFDSLNQNCKKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQ 393
               HY EVM+ +    V +F  L +  + E++ V++Q+P EP K+L  TLRL + E + 
Sbjct: 308 AFNYHYHEVMEEIADTMVQIFKGLQERFQTEIQTVNKQFPCEPFKFLEPTLRLEYCEALA 367

Query: 394 MLKDAGVQIDPLGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCHDNPTYS 453
           ML++AGV++    DL+T  E+ LG L+ EKY T+FYIL +YPLAVRPFYTMP   NP  S
Sbjct: 368 MLREAGVEMGDEDDLSTPNEKLLGHLIKEKYDTDFYILDKYPLAVRPFYTMPDPRNPKQS 427

Query: 454 NSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIEVKTIETYIDSFRYGAPPHGGFGVGL 513
           NS+D+F+RGEEI+SGAQR+H P+ L ERA   GI+++ I+ YIDSFR+GAPPH G G+GL
Sbjct: 428 NSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGAPPHAGGGIGL 487

Query: 514 ERVVMLFCGLNNIRKTSLFPRDPQRIAP 541
           ERV MLF GL+N+R+TS+FPRDP+R+ P
Sbjct: 488 ERVTMLFLGLHNVRQTSMFPRDPKRLTP 501

BLAST of MC11g0497 vs. ExPASy Swiss-Prot
Match: P14868 (Aspartate--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=DARS1 PE=1 SV=2)

HSP 1 Score: 477.6 bits (1228), Expect = 1.8e-133
Identity = 256/508 (50.39%), Postives = 343/508 (67.52%), Query Frame = 0

Query: 34  RRRQEAAAEAAISSLNVEEDPLSANYGDVPLSDLQSKEAKNIENWTQVGSLTEVLKDKYV 93
           R+ QE   E     ++  ED     YG   +S +   + K      +V  LT    D+ V
Sbjct: 8   RKSQEKPREI----MDAAEDYAKERYG---ISSMIQSQEKPDRVLVRVRDLTIQKADEVV 67

Query: 94  LLRGRIQTIRAVAKKMAFLVMREKGFTVQCVVSEQPEVVSRQMVKYVVGLSRESIVDVEG 153
            +R R+ T RA  K+  FLV+R++ F VQ +V+   +  S+QMVK+   +++ESIVDVEG
Sbjct: 68  WVRARVHTSRAKGKQ-CFLVLRQQQFNVQALVA-VGDHASKQMVKFAANINKESIVDVEG 127

Query: 154 VVSVPNVAIKGATQQ-VEIQVRKVYCISKAMPSLPINIEDAARSEVEIEKALQAGEQLVR 213
           VV   N  I   TQQ VE+ V+K+Y IS A P LP+ ++DA R E E E+     E    
Sbjct: 128 VVRKVNQKIGSCTQQDVELHVQKIYVISLAEPRLPLQLDDAVRPEAEGEE-----EGRAT 187

Query: 214 VNQDTRLNYRVLDIRTPANQGIFRIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGAS 273
           VNQDTRL+ RV+D+RT  +Q +FR+Q  +  +FR+ L+++GFVEI TPK+I  +SEGGA+
Sbjct: 188 VNQDTRLDNRVIDLRTSTSQAVFRLQSGICHLFRETLINKGFVEIQTPKIISAASEGGAN 247

Query: 274 VFKLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEM 333
           VF + Y    A LAQSPQL+KQM IC DF +VF IGPVFRAEDS THRHL EF GLD+EM
Sbjct: 248 VFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFSIGPVFRAEDSNTHRHLTEFVGLDIEM 307

Query: 334 ELKTHYSEVMDIVDRLFVSMFDSLNQNCKKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQ 393
               HY EVM+ +    V +F  L +  + E++ V++Q+P EP K+L  TLRL + E + 
Sbjct: 308 AFNYHYHEVMEEIADTMVQIFKGLQERFQTEIQTVNKQFPCEPFKFLEPTLRLEYCEALA 367

Query: 394 MLKDAGVQIDPLGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCHDNPTYS 453
           ML++AGV++    DL+T  E+ LG LV EKY T+FYIL +YPLAVRPFYTMP   NP  S
Sbjct: 368 MLREAGVEMGDEDDLSTPNEKLLGHLVKEKYDTDFYILDKYPLAVRPFYTMPDPRNPKQS 427

Query: 454 NSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIEVKTIETYIDSFRYGAPPHGGFGVGL 513
           NS+D+F+RGEEI+SGAQR+H P+ L ERA   GI+++ I+ YIDSFR+GAPPH G G+GL
Sbjct: 428 NSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGAPPHAGGGIGL 487

Query: 514 ERVVMLFCGLNNIRKTSLFPRDPQRIAP 541
           ERV MLF GL+N+R+TS+FPRDP+R+ P
Sbjct: 488 ERVTMLFLGLHNVRQTSMFPRDPKRLTP 501

BLAST of MC11g0497 vs. ExPASy Swiss-Prot
Match: Q922B2 (Aspartate--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Dars1 PE=1 SV=2)

HSP 1 Score: 476.1 bits (1224), Expect = 5.3e-133
Identity = 256/508 (50.39%), Postives = 345/508 (67.91%), Query Frame = 0

Query: 34  RRRQEAAAEAAISSLNVEEDPLSANYGDVPLSDLQSKEAKNIENWTQVGSLTEVLKDKYV 93
           R+ QE   E     ++  ED     YG   +S +   + K      +V  LT    D  V
Sbjct: 8   RKGQEKPREI----VDAAEDYAKERYG---ISSMIQSQEKPDRVLVRVKDLTVQKADDVV 67

Query: 94  LLRGRIQTIRAVAKKMAFLVMREKGFTVQCVVSEQPEVVSRQMVKYVVGLSRESIVDVEG 153
            +R R+ T RA  K+  FLV+R++ F VQ +V+   +  S+QMVK+   +++ESI+DVEG
Sbjct: 68  WVRARVHTSRAKGKQ-CFLVLRQQQFNVQALVA-VGDHASKQMVKFAANINKESIIDVEG 127

Query: 154 VVSVPNVAIKGATQQ-VEIQVRKVYCISKAMPSLPINIEDAARSEVEIEKALQAGEQLVR 213
           VV   N  I   TQQ VE+ V+K+Y IS A P LP+ ++DA R EVE E+  +A      
Sbjct: 128 VVRKVNQKIGSCTQQDVELHVQKIYVISLAEPRLPLQLDDAIRPEVEGEEDGRA-----T 187

Query: 214 VNQDTRLNYRVLDIRTPANQGIFRIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGAS 273
           VNQDTRL+ RV+D+RT  +Q IFR+Q  +  +FR+ L+++GFVEI TPK+I  +SEGGA+
Sbjct: 188 VNQDTRLDNRVIDLRTSTSQAIFRLQSGICHLFRETLINKGFVEIQTPKIISAASEGGAN 247

Query: 274 VFKLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEM 333
           VF + Y    A LAQSPQL+KQM IC DF +VF IGPVFRAEDS THRHL EF GLD+EM
Sbjct: 248 VFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFCIGPVFRAEDSNTHRHLTEFVGLDIEM 307

Query: 334 ELKTHYSEVMDIVDRLFVSMFDSLNQNCKKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQ 393
               HY EV++ +    V +F  L +  + E++ V +Q+P EP K+L  TLRL + E + 
Sbjct: 308 AFNYHYHEVVEEIADTLVQIFKGLQERFQTEIQTVSKQFPCEPFKFLEPTLRLEYCEALA 367

Query: 394 MLKDAGVQIDPLGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCHDNPTYS 453
           ML++AGV++D   DL+T  E+ LG+LV EKY T+FY+L +YPLAVRPFYTMP   NP  S
Sbjct: 368 MLREAGVEMDDEEDLSTPNEKLLGRLVKEKYDTDFYVLDKYPLAVRPFYTMPDPRNPKQS 427

Query: 454 NSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIEVKTIETYIDSFRYGAPPHGGFGVGL 513
           NS+D+F+RGEEI+SGAQR+H P+ L ERA   GI+++ I+ YIDSFR+GAPPH G G+GL
Sbjct: 428 NSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGAPPHAGGGIGL 487

Query: 514 ERVVMLFCGLNNIRKTSLFPRDPQRIAP 541
           ERV MLF GL+N+R+TS+FPRDP+R+ P
Sbjct: 488 ERVTMLFLGLHNVRQTSMFPRDPKRLTP 501

BLAST of MC11g0497 vs. NCBI nr
Match: XP_022138313.1 (aspartate--tRNA ligase 2, cytoplasmic [Momordica charantia])

HSP 1 Score: 1053 bits (2724), Expect = 0.0
Identity = 540/540 (100.00%), Postives = 540/540 (100.00%), Query Frame = 0

Query: 1   MSAPSETPIEEPPSAGAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPLSANYG 60
           MSAPSETPIEEPPSAGAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPLSANYG
Sbjct: 1   MSAPSETPIEEPPSAGAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPLSANYG 60

Query: 61  DVPLSDLQSKEAKNIENWTQVGSLTEVLKDKYVLLRGRIQTIRAVAKKMAFLVMREKGFT 120
           DVPLSDLQSKEAKNIENWTQVGSLTEVLKDKYVLLRGRIQTIRAVAKKMAFLVMREKGFT
Sbjct: 61  DVPLSDLQSKEAKNIENWTQVGSLTEVLKDKYVLLRGRIQTIRAVAKKMAFLVMREKGFT 120

Query: 121 VQCVVSEQPEVVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCIS 180
           VQCVVSEQPEVVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCIS
Sbjct: 121 VQCVVSEQPEVVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCIS 180

Query: 181 KAMPSLPINIEDAARSEVEIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIFRIQCQ 240
           KAMPSLPINIEDAARSEVEIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIFRIQCQ
Sbjct: 181 KAMPSLPINIEDAARSEVEIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIFRIQCQ 240

Query: 241 VGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFKLDYKGQPACLAQSPQLHKQMAICGD 300
           VGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFKLDYKGQPACLAQSPQLHKQMAICGD
Sbjct: 241 VGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFKLDYKGQPACLAQSPQLHKQMAICGD 300

Query: 301 FGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMELKTHYSEVMDIVDRLFVSMFDSLNQNC 360
           FGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMELKTHYSEVMDIVDRLFVSMFDSLNQNC
Sbjct: 301 FGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMELKTHYSEVMDIVDRLFVSMFDSLNQNC 360

Query: 361 KKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQMLKDAGVQIDPLGDLNTEAERKLGQLVL 420
           KKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQMLKDAGVQIDPLGDLNTEAERKLGQLVL
Sbjct: 361 KKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQMLKDAGVQIDPLGDLNTEAERKLGQLVL 420

Query: 421 EKYGTEFYILHRYPLAVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAER 480
           EKYGTEFYILHRYPLAVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAER
Sbjct: 421 EKYGTEFYILHRYPLAVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAER 480

Query: 481 AQACGIEVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 540
           AQACGIEVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP
Sbjct: 481 AQACGIEVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 540

BLAST of MC11g0497 vs. NCBI nr
Match: XP_023535979.1 (aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1008 bits (2607), Expect = 0.0
Identity = 514/545 (94.31%), Postives = 532/545 (97.61%), Query Frame = 0

Query: 1   MSAPSETPIEEPPSAG-----AVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPL 60
           MSA SETPI+EP SAG     AVSKKAAKKEAAK EKLRRRQEA AEAAISSLNVE+DPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAVAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEAKNIENWTQVGSLTEVLKDKYVLLRGRIQTIRAVAKKMAFLVMR 120
           SANYGDVPLSDLQSKEAK++ NWTQVGSLT  LKDKYV+LRGRIQTIRAVAKKMAFLV+R
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 EKGFTVQCVVSEQPEVVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180
           EKGFTVQCVVSEQPE+VSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPSLPINIEDAARSEVEIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIF 240
           VYCISKAMPSLPINIEDAARSE EIEKALQAGEQLVRVNQDTRLNYRVLD+RTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFKLDYKGQPACLAQSPQLHKQM 300
           RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVF+LDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMELKTHYSEVMDIVDRLFVSMFDS 360
           AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEME+KTHYSEVMDIVDRLFVSMFDS
Sbjct: 301 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 361 LNQNCKKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQMLKDAGVQIDPLGDLNTEAERKL 420
           +NQNCKKELEA+D+QYPFEPLKYLRNTLRLTFEEG+QMLKDAGV+IDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIEVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGI+VKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

BLAST of MC11g0497 vs. NCBI nr
Match: KAG6591612.1 (Aspartate--tRNA ligase 2, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1007 bits (2604), Expect = 0.0
Identity = 513/545 (94.13%), Postives = 533/545 (97.80%), Query Frame = 0

Query: 1   MSAPSETPIEEPPSAG-----AVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPL 60
           MSA SETPI+EP SAG     AVSKKAAKKEAAK EKLRRRQEAAAEAAISSLNVE+DPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEAKNIENWTQVGSLTEVLKDKYVLLRGRIQTIRAVAKKMAFLVMR 120
           SANYGDVPLSDLQSKEAK++ NWTQVGSLT  LK+KYV+LRGRIQTIRAVAKKMAFLV+R
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKEKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 EKGFTVQCVVSEQPEVVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180
           EKGFTVQCVVSEQPE+VSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPSLPINIEDAARSEVEIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIF 240
           VYCISKAMPSLPINIEDAARSE EIEKALQAGEQLVRVNQDTRLNYRVLD+RTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFKLDYKGQPACLAQSPQLHKQM 300
           RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVF+LDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMELKTHYSEVMDIVDRLFVSMFDS 360
           +ICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEME+KTHYSEVMDIVDRLFVSMFDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 361 LNQNCKKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQMLKDAGVQIDPLGDLNTEAERKL 420
           +NQNCKKELEA+D+QYPFEPLKYLRNTLRLTFEEG+QMLKDAGV+IDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIEVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGI+VKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

BLAST of MC11g0497 vs. NCBI nr
Match: XP_022936995.1 (aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita moschata])

HSP 1 Score: 1007 bits (2604), Expect = 0.0
Identity = 513/545 (94.13%), Postives = 532/545 (97.61%), Query Frame = 0

Query: 1   MSAPSETPIEEPPSAG-----AVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPL 60
           MSA SETPI+EP SAG     AVSKKAAKKEAAK EKLRRRQEAAAEAAISSLNVE+DPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEAKNIENWTQVGSLTEVLKDKYVLLRGRIQTIRAVAKKMAFLVMR 120
           SANYGDVPLSDLQSKEAK++ NWTQVGSLT  LKDKYV+LRGRIQTIRAVAKKMAFLV+R
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 EKGFTVQCVVSEQPEVVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180
           EKGFTVQCVVSEQPE+VSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPSLPINIEDAARSEVEIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIF 240
           VYCISKAMPSLPINIEDAARSE EIEKALQAGEQLVRVNQDTRLNYRVLD+RTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFKLDYKGQPACLAQSPQLHKQM 300
           RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVF+LDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMELKTHYSEVMDIVDRLFVSMFDS 360
           +ICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEME+KTHYSEVMDIVDRLFVSMFDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 361 LNQNCKKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQMLKDAGVQIDPLGDLNTEAERKL 420
           +NQNCKKELEA+D+QYPFEPLKYLRNTLRLTFEEG+QMLKDAGV+IDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIEVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FL ERAQACGI+VKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLTERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

BLAST of MC11g0497 vs. NCBI nr
Match: XP_022976148.1 (aspartate--tRNA ligase 2, cytoplasmic-like [Cucurbita maxima])

HSP 1 Score: 1003 bits (2594), Expect = 0.0
Identity = 512/545 (93.94%), Postives = 531/545 (97.43%), Query Frame = 0

Query: 1   MSAPSETPIEEPPSAG-----AVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPL 60
           MSA SETPI+E  SAG     AVSKKAAKKEAAK EKLRRRQEAAAEAAISSLNVE+DPL
Sbjct: 1   MSASSETPIDETLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEAKNIENWTQVGSLTEVLKDKYVLLRGRIQTIRAVAKKMAFLVMR 120
           SANYGDVPLSDLQSKEAK++ NWTQVGSLT  LKDKYV+LRGRIQTIRAVAKKMAFLV+R
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 EKGFTVQCVVSEQPEVVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180
           EKGFTVQCVVSEQPE+VSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPSLPINIEDAARSEVEIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIF 240
           VYCISKAMPSLPINIEDAARSE EIEKALQAGEQLVRVNQDTRLNYRVLD+RTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFKLDYKGQPACLAQSPQLHKQM 300
           RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVF+LDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMELKTHYSEVMDIVDRLFVSMFDS 360
           +ICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEME+KTHY EVMDIVDRLFVSMFDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYYEVMDIVDRLFVSMFDS 360

Query: 361 LNQNCKKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQMLKDAGVQIDPLGDLNTEAERKL 420
           +NQNCKKELEA+D+QYPFEPLKYLRNTLRLTFEEG+QMLKDAGV+IDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIEVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGI+VKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

BLAST of MC11g0497 vs. ExPASy TrEMBL
Match: A0A6J1CCP4 (Aspartate--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111009525 PE=3 SV=1)

HSP 1 Score: 1053 bits (2724), Expect = 0.0
Identity = 540/540 (100.00%), Postives = 540/540 (100.00%), Query Frame = 0

Query: 1   MSAPSETPIEEPPSAGAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPLSANYG 60
           MSAPSETPIEEPPSAGAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPLSANYG
Sbjct: 1   MSAPSETPIEEPPSAGAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPLSANYG 60

Query: 61  DVPLSDLQSKEAKNIENWTQVGSLTEVLKDKYVLLRGRIQTIRAVAKKMAFLVMREKGFT 120
           DVPLSDLQSKEAKNIENWTQVGSLTEVLKDKYVLLRGRIQTIRAVAKKMAFLVMREKGFT
Sbjct: 61  DVPLSDLQSKEAKNIENWTQVGSLTEVLKDKYVLLRGRIQTIRAVAKKMAFLVMREKGFT 120

Query: 121 VQCVVSEQPEVVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCIS 180
           VQCVVSEQPEVVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCIS
Sbjct: 121 VQCVVSEQPEVVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCIS 180

Query: 181 KAMPSLPINIEDAARSEVEIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIFRIQCQ 240
           KAMPSLPINIEDAARSEVEIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIFRIQCQ
Sbjct: 181 KAMPSLPINIEDAARSEVEIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIFRIQCQ 240

Query: 241 VGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFKLDYKGQPACLAQSPQLHKQMAICGD 300
           VGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFKLDYKGQPACLAQSPQLHKQMAICGD
Sbjct: 241 VGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFKLDYKGQPACLAQSPQLHKQMAICGD 300

Query: 301 FGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMELKTHYSEVMDIVDRLFVSMFDSLNQNC 360
           FGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMELKTHYSEVMDIVDRLFVSMFDSLNQNC
Sbjct: 301 FGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMELKTHYSEVMDIVDRLFVSMFDSLNQNC 360

Query: 361 KKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQMLKDAGVQIDPLGDLNTEAERKLGQLVL 420
           KKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQMLKDAGVQIDPLGDLNTEAERKLGQLVL
Sbjct: 361 KKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQMLKDAGVQIDPLGDLNTEAERKLGQLVL 420

Query: 421 EKYGTEFYILHRYPLAVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAER 480
           EKYGTEFYILHRYPLAVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAER
Sbjct: 421 EKYGTEFYILHRYPLAVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAER 480

Query: 481 AQACGIEVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 540
           AQACGIEVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP
Sbjct: 481 AQACGIEVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 540

BLAST of MC11g0497 vs. ExPASy TrEMBL
Match: A0A6J1FFA5 (Aspartate--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111443419 PE=3 SV=1)

HSP 1 Score: 1007 bits (2604), Expect = 0.0
Identity = 513/545 (94.13%), Postives = 532/545 (97.61%), Query Frame = 0

Query: 1   MSAPSETPIEEPPSAG-----AVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPL 60
           MSA SETPI+EP SAG     AVSKKAAKKEAAK EKLRRRQEAAAEAAISSLNVE+DPL
Sbjct: 1   MSASSETPIDEPLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEAKNIENWTQVGSLTEVLKDKYVLLRGRIQTIRAVAKKMAFLVMR 120
           SANYGDVPLSDLQSKEAK++ NWTQVGSLT  LKDKYV+LRGRIQTIRAVAKKMAFLV+R
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 EKGFTVQCVVSEQPEVVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180
           EKGFTVQCVVSEQPE+VSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPSLPINIEDAARSEVEIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIF 240
           VYCISKAMPSLPINIEDAARSE EIEKALQAGEQLVRVNQDTRLNYRVLD+RTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFKLDYKGQPACLAQSPQLHKQM 300
           RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVF+LDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMELKTHYSEVMDIVDRLFVSMFDS 360
           +ICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEME+KTHYSEVMDIVDRLFVSMFDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYSEVMDIVDRLFVSMFDS 360

Query: 361 LNQNCKKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQMLKDAGVQIDPLGDLNTEAERKL 420
           +NQNCKKELEA+D+QYPFEPLKYLRNTLRLTFEEG+QMLKDAGV+IDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIEVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FL ERAQACGI+VKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLTERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

BLAST of MC11g0497 vs. ExPASy TrEMBL
Match: A0A6J1ILA3 (Aspartate--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111476621 PE=3 SV=1)

HSP 1 Score: 1003 bits (2594), Expect = 0.0
Identity = 512/545 (93.94%), Postives = 531/545 (97.43%), Query Frame = 0

Query: 1   MSAPSETPIEEPPSAG-----AVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPL 60
           MSA SETPI+E  SAG     AVSKKAAKKEAAK EKLRRRQEAAAEAAISSLNVE+DPL
Sbjct: 1   MSASSETPIDETLSAGDEASKAVSKKAAKKEAAKLEKLRRRQEAAAEAAISSLNVEDDPL 60

Query: 61  SANYGDVPLSDLQSKEAKNIENWTQVGSLTEVLKDKYVLLRGRIQTIRAVAKKMAFLVMR 120
           SANYGDVPLSDLQSKEAK++ NWTQVGSLT  LKDKYV+LRGRIQTIRAVAKKMAFLV+R
Sbjct: 61  SANYGDVPLSDLQSKEAKDVANWTQVGSLTGELKDKYVILRGRIQTIRAVAKKMAFLVVR 120

Query: 121 EKGFTVQCVVSEQPEVVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180
           EKGFTVQCVVSEQPE+VSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK
Sbjct: 121 EKGFTVQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRK 180

Query: 181 VYCISKAMPSLPINIEDAARSEVEIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIF 240
           VYCISKAMPSLPINIEDAARSE EIEKALQAGEQLVRVNQDTRLNYRVLD+RTPANQGIF
Sbjct: 181 VYCISKAMPSLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDMRTPANQGIF 240

Query: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFKLDYKGQPACLAQSPQLHKQM 300
           RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVF+LDYKGQPACLAQSPQLHKQM
Sbjct: 241 RIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQM 300

Query: 301 AICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMELKTHYSEVMDIVDRLFVSMFDS 360
           +ICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEME+KTHY EVMDIVDRLFVSMFDS
Sbjct: 301 SICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMEIKTHYYEVMDIVDRLFVSMFDS 360

Query: 361 LNQNCKKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQMLKDAGVQIDPLGDLNTEAERKL 420
           +NQNCKKELEA+D+QYPFEPLKYLRNTLRLTFEEG+QMLKDAGV+IDPLGDLNTEAERKL
Sbjct: 361 MNQNCKKELEAIDKQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKL 420

Query: 421 GQLVLEKYGTEFYILHRYPLAVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480
           G+LVLEKYGTEFYILHRYPL+VRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE
Sbjct: 421 GRLVLEKYGTEFYILHRYPLSVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPE 480

Query: 481 FLAERAQACGIEVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540
           FLAERAQACGI+VKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP
Sbjct: 481 FLAERAQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 540

BLAST of MC11g0497 vs. ExPASy TrEMBL
Match: A0A6J1FKT3 (Aspartate--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111446655 PE=3 SV=1)

HSP 1 Score: 1003 bits (2593), Expect = 0.0
Identity = 511/540 (94.63%), Postives = 530/540 (98.15%), Query Frame = 0

Query: 1   MSAPSETPIEEPPSAGAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPLSANYG 60
           MSA SETP++E  SAG VSKKAAKKEAAKQEKLRRRQEAAAE AISSL+VE+DPLSANYG
Sbjct: 1   MSASSETPMDESLSAG-VSKKAAKKEAAKQEKLRRRQEAAAEVAISSLDVEDDPLSANYG 60

Query: 61  DVPLSDLQSKEAKNIENWTQVGSLTEVLKDKYVLLRGRIQTIRAVAKKMAFLVMREKGFT 120
           DVPLS+LQSKEAKNIENWTQVGSLT  LKDKYVLLRGR+QTIRAVAKKMAFLV+REKGFT
Sbjct: 61  DVPLSNLQSKEAKNIENWTQVGSLTGELKDKYVLLRGRVQTIRAVAKKMAFLVVREKGFT 120

Query: 121 VQCVVSEQPEVVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCIS 180
           VQCVVSEQPE+VSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCIS
Sbjct: 121 VQCVVSEQPELVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCIS 180

Query: 181 KAMPSLPINIEDAARSEVEIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIFRIQCQ 240
           KAMP+LPINIEDAARSE EIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIFRIQCQ
Sbjct: 181 KAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIFRIQCQ 240

Query: 241 VGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFKLDYKGQPACLAQSPQLHKQMAICGD 300
           VG IFRQFLLSEGFVEIHTPKLIGGSSEGGASVF+LDYKGQPACLAQSPQLHKQMAICGD
Sbjct: 241 VGAIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQMAICGD 300

Query: 301 FGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMELKTHYSEVMDIVDRLFVSMFDSLNQNC 360
           FGRVFEIGPVFRAEDS+THRHLCEFTGLDVEME+KTHYSEVM+IVDRLFV MFDSLN+NC
Sbjct: 301 FGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYSEVMNIVDRLFVKMFDSLNENC 360

Query: 361 KKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQMLKDAGVQIDPLGDLNTEAERKLGQLVL 420
           KKELEA+DRQYPFEPLKYLRNTLRLTFEEG+QMLKDAGV+IDPLGDLNTEAERKLGQLVL
Sbjct: 361 KKELEAIDRQYPFEPLKYLRNTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKLGQLVL 420

Query: 421 EKYGTEFYILHRYPLAVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAER 480
           EKYGTEFYILHRYPLAVRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAER
Sbjct: 421 EKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAER 480

Query: 481 AQACGIEVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 540
           AQACGI+VKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP
Sbjct: 481 AQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 539

BLAST of MC11g0497 vs. ExPASy TrEMBL
Match: A0A6J1J3D8 (Aspartate--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111480983 PE=3 SV=1)

HSP 1 Score: 995 bits (2572), Expect = 0.0
Identity = 506/540 (93.70%), Postives = 528/540 (97.78%), Query Frame = 0

Query: 1   MSAPSETPIEEPPSAGAVSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEEDPLSANYG 60
           MSA SETP++E  SAG VSKKAAKKEAAKQEKLRRRQEAAAE +ISSL+VE+DPLSANYG
Sbjct: 1   MSASSETPMDESLSAG-VSKKAAKKEAAKQEKLRRRQEAAAEVSISSLDVEDDPLSANYG 60

Query: 61  DVPLSDLQSKEAKNIENWTQVGSLTEVLKDKYVLLRGRIQTIRAVAKKMAFLVMREKGFT 120
           DVPLS+LQSKEAKNI+NWTQVGSLT  LKDKYVLLRGR+QTIRAVAKKMAFLV+REKGFT
Sbjct: 61  DVPLSNLQSKEAKNIDNWTQVGSLTGELKDKYVLLRGRVQTIRAVAKKMAFLVVREKGFT 120

Query: 121 VQCVVSEQPEVVSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCIS 180
           VQCVVSEQPE++SRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCIS
Sbjct: 121 VQCVVSEQPELLSRQMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCIS 180

Query: 181 KAMPSLPINIEDAARSEVEIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIFRIQCQ 240
           KAMP+LPINIEDAARSE EIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIFRIQCQ
Sbjct: 181 KAMPTLPINIEDAARSEAEIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIFRIQCQ 240

Query: 241 VGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFKLDYKGQPACLAQSPQLHKQMAICGD 300
           VG IFRQFLLSEGFVEIHTPKLIGGSSEGGASVF+LDYKGQPACLAQSPQLHKQMAICGD
Sbjct: 241 VGAIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFRLDYKGQPACLAQSPQLHKQMAICGD 300

Query: 301 FGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMELKTHYSEVMDIVDRLFVSMFDSLNQNC 360
           FGRVFEIGPVFRAEDS+THRHLCEFTGLDVEME+KTHY EVM+IVDRLFV MFDSLN+NC
Sbjct: 301 FGRVFEIGPVFRAEDSYTHRHLCEFTGLDVEMEIKTHYFEVMNIVDRLFVKMFDSLNENC 360

Query: 361 KKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQMLKDAGVQIDPLGDLNTEAERKLGQLVL 420
           KKELEA+DRQYPFEPLKYLR TLRLTFEEG+QMLKDAGV+IDPLGDLNTEAERKLGQLVL
Sbjct: 361 KKELEAIDRQYPFEPLKYLRTTLRLTFEEGIQMLKDAGVEIDPLGDLNTEAERKLGQLVL 420

Query: 421 EKYGTEFYILHRYPLAVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAER 480
           EKYGTEFYILHRYPLAVRPFYTMPC+DNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAER
Sbjct: 421 EKYGTEFYILHRYPLAVRPFYTMPCYDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAER 480

Query: 481 AQACGIEVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 540
           AQACGI+VKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP
Sbjct: 481 AQACGIDVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 539

BLAST of MC11g0497 vs. TAIR 10
Match: AT4G31180.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 731.5 bits (1887), Expect = 5.0e-211
Identity = 379/558 (67.92%), Postives = 440/558 (78.85%), Query Frame = 0

Query: 1   MSAPSETPIEEPPSAGA------VSKKAAKKEAAKQEKLRRRQE-AAAEAAISSLNVEE- 60
           MS+ SE P     +A A       SKKAAKKEAAK EKLRRRQE   A    +S+++EE 
Sbjct: 1   MSSESEIPPLSSSTAAAEESGEKTSKKAAKKEAAKLEKLRRRQEQEEATRRTASISLEEN 60

Query: 61  DPLSANYGDVPLSDLQSK---------EAKNIENWTQVGSLTEVLKDKYVLLRGRIQTIR 120
           D  S NYGDV L++LQS          EA   + WT V  L E + +  VL+RGR+ T R
Sbjct: 61  DEFSNNYGDVTLTELQSSADPKAGKWIEAVEGKEWTDVSDLVEEMLESEVLIRGRVHTNR 120

Query: 121 AVAKKMAFLVMREKGFTVQCVVSEQPEV-VSRQMVKYVVGLSRESIVDVEGVVSVPNVAI 180
             + K+ F+V+RE G TVQCVVS+  +  V   MVKY+  LSRES VDV GVV++P   +
Sbjct: 121 PTSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSRESFVDVIGVVTLPKEPL 180

Query: 181 KGATQQVEIQVRKVYCISKAMPSLPINIEDAARSEVEIEKALQAGEQLVRVNQDTRLNYR 240
            G TQQVEIQVRKVYCI+K++  LP+++EDAARSE +IE +LQ      RVNQDTRLNYR
Sbjct: 181 TGTTQQVEIQVRKVYCINKSLAKLPLSVEDAARSEADIEASLQTPSPAARVNQDTRLNYR 240

Query: 241 VLDIRTPANQGIFRIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFKLDYKGQP 300
           VLD+RTPANQ IF++Q +V   FR+ L  + FV IHTPKL+ GSSEGG++VF+L+YKGQP
Sbjct: 241 VLDLRTPANQAIFQLQYEVEYAFREKLRFKNFVGIHTPKLMAGSSEGGSAVFRLEYKGQP 300

Query: 301 ACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMELKTHYSEVM 360
           ACLAQSPQLHKQMAICGD  RVFE+GPVFRAEDSFTHRHLCEF GLDVEME++ HYSE+M
Sbjct: 301 ACLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRKHYSEIM 360

Query: 361 DIVDRLFVSMFDSLNQNCKKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQMLKDAGVQID 420
           D+VD LFV +F SLN+ CKKEL+AV +QYPFEPLK+L  TLRLTFEEGVQMLK+AGV++D
Sbjct: 361 DLVDELFVFIFTSLNERCKKELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEVD 420

Query: 421 PLGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCHDNPTYSNSFDVFIRGE 480
           PLGDLNTE+ERKLGQLVLEKY TEFYILHRYP AVRPFYTM C DNP YSNSFDVFIRGE
Sbjct: 421 PLGDLNTESERKLGQLVLEKYNTEFYILHRYPKAVRPFYTMTCADNPLYSNSFDVFIRGE 480

Query: 481 EIISGAQRVHVPEFLAERAQACGIEVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGL 540
           EIISGAQRVH+PE L +RA  CGI+VKTI TYIDSFRYGAP HGGFGVGLERVVMLFC L
Sbjct: 481 EIISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCAL 540

BLAST of MC11g0497 vs. TAIR 10
Match: AT4G31180.2 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 731.5 bits (1887), Expect = 5.0e-211
Identity = 379/558 (67.92%), Postives = 440/558 (78.85%), Query Frame = 0

Query: 1   MSAPSETPIEEPPSAGA------VSKKAAKKEAAKQEKLRRRQE-AAAEAAISSLNVEE- 60
           MS+ SE P     +A A       SKKAAKKEAAK EKLRRRQE   A    +S+++EE 
Sbjct: 1   MSSESEIPPLSSSTAAAEESGEKTSKKAAKKEAAKLEKLRRRQEQEEATRRTASISLEEN 60

Query: 61  DPLSANYGDVPLSDLQSK---------EAKNIENWTQVGSLTEVLKDKYVLLRGRIQTIR 120
           D  S NYGDV L++LQS          EA   + WT V  L E + +  VL+RGR+ T R
Sbjct: 61  DEFSNNYGDVTLTELQSSADPKAGKWIEAVEGKEWTDVSDLVEEMLESEVLIRGRVHTNR 120

Query: 121 AVAKKMAFLVMREKGFTVQCVVSEQPEV-VSRQMVKYVVGLSRESIVDVEGVVSVPNVAI 180
             + K+ F+V+RE G TVQCVVS+  +  V   MVKY+  LSRES VDV GVV++P   +
Sbjct: 121 PTSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSRESFVDVIGVVTLPKEPL 180

Query: 181 KGATQQVEIQVRKVYCISKAMPSLPINIEDAARSEVEIEKALQAGEQLVRVNQDTRLNYR 240
            G TQQVEIQVRKVYCI+K++  LP+++EDAARSE +IE +LQ      RVNQDTRLNYR
Sbjct: 181 TGTTQQVEIQVRKVYCINKSLAKLPLSVEDAARSEADIEASLQTPSPAARVNQDTRLNYR 240

Query: 241 VLDIRTPANQGIFRIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGASVFKLDYKGQP 300
           VLD+RTPANQ IF++Q +V   FR+ L  + FV IHTPKL+ GSSEGG++VF+L+YKGQP
Sbjct: 241 VLDLRTPANQAIFQLQYEVEYAFREKLRFKNFVGIHTPKLMAGSSEGGSAVFRLEYKGQP 300

Query: 301 ACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMELKTHYSEVM 360
           ACLAQSPQLHKQMAICGD  RVFE+GPVFRAEDSFTHRHLCEF GLDVEME++ HYSE+M
Sbjct: 301 ACLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRKHYSEIM 360

Query: 361 DIVDRLFVSMFDSLNQNCKKELEAVDRQYPFEPLKYLRNTLRLTFEEGVQMLKDAGVQID 420
           D+VD LFV +F SLN+ CKKEL+AV +QYPFEPLK+L  TLRLTFEEGVQMLK+AGV++D
Sbjct: 361 DLVDELFVFIFTSLNERCKKELQAVGKQYPFEPLKFLPKTLRLTFEEGVQMLKEAGVEVD 420

Query: 421 PLGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCHDNPTYSNSFDVFIRGE 480
           PLGDLNTE+ERKLGQLVLEKY TEFYILHRYP AVRPFYTM C DNP YSNSFDVFIRGE
Sbjct: 421 PLGDLNTESERKLGQLVLEKYNTEFYILHRYPKAVRPFYTMTCADNPLYSNSFDVFIRGE 480

Query: 481 EIISGAQRVHVPEFLAERAQACGIEVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGL 540
           EIISGAQRVH+PE L +RA  CGI+VKTI TYIDSFRYGAP HGGFGVGLERVVMLFC L
Sbjct: 481 EIISGAQRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCAL 540

BLAST of MC11g0497 vs. TAIR 10
Match: AT4G26870.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 687.6 bits (1773), Expect = 8.2e-198
Identity = 351/526 (66.73%), Postives = 421/526 (80.04%), Query Frame = 0

Query: 18  VSKKAAKKEAAKQEKLRRRQEAAAEAAISSLNVEE--DPLSANYGDVPLSDLQSK-EAKN 77
           +SKK +KK AAK EKL R+QE   EA  SSL++EE  +  S+NYGDV  ++LQS  E K 
Sbjct: 14  ISKKESKKRAAKLEKLLRKQE-REEATSSSLSLEEEDESCSSNYGDVTTNELQSAVEGKE 73

Query: 78  IENWTQVGSLTEVLKDKYVLLRGRIQTIRAVAKKMAFLVMREKGFTVQCVVSEQPEVVSR 137
           +   T V +L E +    V +RGR+   R V  K+ F+++RE GFTVQCVV E    V  
Sbjct: 74  L---TDVSNLVEEIVGSEVSIRGRLHKNRLVGTKL-FVILRESGFTVQCVVEETR--VGA 133

Query: 138 QMVKYVVGLSRESIVDVEGVVSVPNVAIKGATQQVEIQVRKVYCISKAMPSLPINIEDAA 197
            M+K+V  LSRES+V++ GVVS P   + G TQQVEI VRK+YC+S+++P+LP+ +EDAA
Sbjct: 134 NMIKFVKQLSRESVVELIGVVSHPKKPLTGTTQQVEIHVRKMYCLSRSLPNLPLVVEDAA 193

Query: 198 RSEVEIEKALQAGEQLVRVNQDTRLNYRVLDIRTPANQGIFRIQCQVGTIFRQFLLSEGF 257
           RSE +IEK+ + G+Q  RV QDTRLN RVLDIRTPANQ IFRIQCQV   FR++L S+GF
Sbjct: 194 RSESDIEKSGKDGKQAARVLQDTRLNNRVLDIRTPANQAIFRIQCQVQIAFREYLQSKGF 253

Query: 258 VEIHTPKLIGGSSEGGASVFKLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGPVFRAE 317
           +EIHTPKLI GSSEGG++VF+LDYKGQPACLAQSPQLHKQMAICGD  RVFE+GPVFRAE
Sbjct: 254 LEIHTPKLIAGSSEGGSAVFRLDYKGQPACLAQSPQLHKQMAICGDMRRVFEVGPVFRAE 313

Query: 318 DSFTHRHLCEFTGLDVEMELKTHYSEVMDIVDRLFVSMFDSLNQNCKKELEAVDRQYPFE 377
           DSFTHRHLCEF GLDVEME++ HYSE+MD+V  LF  +F  + + C KELE+V +QYPF+
Sbjct: 314 DSFTHRHLCEFVGLDVEMEIRMHYSEIMDLVGELFPFIFTKIEERCPKELESVRKQYPFQ 373

Query: 378 PLKYLRNTLRLTFEEGVQMLKDAGVQIDPLGDLNTEAERKLGQLVLEKYGTEFYILHRYP 437
            LK+L  TLRLTF EG+QMLK+AG ++DPLGDLNTE+ERKLGQLVLEKY TEFY+LHRYP
Sbjct: 374 SLKFLPQTLRLTFAEGIQMLKEAGEEVDPLGDLNTESERKLGQLVLEKYKTEFYMLHRYP 433

Query: 438 LAVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVHVPEFLAERAQACGIEVKTIETY 497
            AVRPFYTMP  ++  YSNSFDVFIRGEEI+SGAQR+H PE L +RA+ CGI+VKTI TY
Sbjct: 434 SAVRPFYTMPYENDSNYSNSFDVFIRGEEIMSGAQRIHDPELLEKRARECGIDVKTISTY 493

Query: 498 IDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDPQRIAP 541
           ID+FRYGAPPHGGFGVGLERVVML C LNNIRKTSLFPRD QR+ P
Sbjct: 494 IDAFRYGAPPHGGFGVGLERVVMLLCALNNIRKTSLFPRDSQRLTP 532

BLAST of MC11g0497 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 125.2 bits (313), Expect = 1.6e-28
Identity = 120/483 (24.84%), Postives = 201/483 (41.61%), Query Frame = 0

Query: 95  LRGRIQTIRAVAKKMAFLVMREKG--FTVQCVVSEQPEVVSR-QMVKYVVGLSRESIVDV 154
           + G ++T+R+    + F+ + +      +QCV++   E   + +    + G S    V V
Sbjct: 114 IMGWVRTLRS-QSSVTFIEINDGSCLSNLQCVMTSDAEGYDQVESGSILTGAS----VSV 173

Query: 155 EGVVSVPNVAIKGATQQVEIQVRKVYCISKAMPSLPINIEDAARSEVEIEKALQAGEQLV 214
           +G +    VA +G  Q+VE++V K+  + +   S PI                    Q  
Sbjct: 174 QGTI----VASQGTKQKVELKVEKIIVVGECDSSYPI--------------------QKK 233

Query: 215 RVNQDTRLNYRVLDIRTPANQGIFRIQCQVGTIFRQFLLSEGFVEIHTPKLIGGSSEGGA 274
           RV+++       L  RT     + R++  +     +F    GFV + +P +     EG  
Sbjct: 234 RVSREFLRTKAHLRPRTNTFGAVARVRNTLAYATHKFFQESGFVWVASPIITASDCEGAG 293

Query: 275 SVF------------------------------KLDYKGQPACLAQSPQLHKQMAICGDF 334
             F                                D+ G+PA L  S QL+ +       
Sbjct: 294 EQFCVTTLIPSSHENTDTSIDAIPKTKGGLIDWSQDFFGKPAFLTVSGQLNGE-TYATAL 353

Query: 335 GRVFEIGPVFRAEDSFTHRHLCEFTGLDVEMELKTHYSEVMDIVDRLFVSMFDSLNQNCK 394
             V+  GP FRAE+S T RHL EF  ++ E+       + M         +   +  NCK
Sbjct: 354 SDVYTFGPTFRAENSNTSRHLAEFWMIEPELAF-ADLDDDMACATAYLQYVVKYVLDNCK 413

Query: 395 KELEAVDRQYPFEPLKYL-----RNTLRLTFEEGVQMLKDAGVQID-PLG---DLNTEAE 454
           +++E  D       ++ L     +  L+L + + +++L  A  + D P+    DL +E E
Sbjct: 414 EDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTDAIEILLKANKKFDFPVKWGLDLQSEHE 473

Query: 455 RKLGQLVLEKYGTEFYILHRYPLAVRPFYTMPCHDNPTYSNSFDVFIRGEEIISGAQRVH 514
           R + +   E +G    I+  YP  ++ FY     D  T +    +  R  E+I G+QR  
Sbjct: 474 RYITE---EAFGGRPVIIRDYPKEIKAFYMRENDDGKTVAAMDMLVPRIGELIGGSQREE 533

Query: 515 VPEFLAERAQACGIEVKTIETYIDSFRYGAPPHGGFGVGLERVVMLFCGLNNIRKTSLFP 536
             E L  R     +  ++   Y+D  RYG+ PH GFG+G ER+V    G++NIR    FP
Sbjct: 534 RLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIRDVIPFP 562

BLAST of MC11g0497 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 122.5 bits (306), Expect = 1.1e-27
Identity = 83/275 (30.18%), Postives = 143/275 (52.00%), Query Frame = 0

Query: 274 FKLDYKGQPACLAQSPQLHKQMAICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLDVEME 333
           +  D+ G+ A L  S QL  +   C     V+  GP FRAE+S T RHL EF  ++ E+ 
Sbjct: 300 YSKDFFGRQAFLTVSGQLQVETYACA-LSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 359

Query: 334 LKTHYSEVMDIVDRLFVSMFDSLNQNCKKELEAVDRQYP---FEPLKYLRNTL--RLTFE 393
                 + M+  +     M + L + C  ++E + + +     + LK + +T   R+T+ 
Sbjct: 360 F-ADLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYT 419

Query: 394 EGVQMLKDA---GVQID---PLG-DLNTEAERKLGQLVLEKYGTEFYILHRYPLAVRPFY 453
           + +++L++A   G + D     G DL +E ER L +++ +K      I++ YP  ++ FY
Sbjct: 420 KAIELLEEAVAKGKEFDNNVEWGIDLASEHERYLTEVLFQKP----LIVYNYPKGIKAFY 479

Query: 454 TMPCHDNPTYSNSFDVFI-RGEEIISGAQRVHVPEFLAERAQACGIEVKTIETYIDSFRY 513
            M  +D+     + DV + +  E+I G+QR    + + +R +  G+ ++  E Y+D  RY
Sbjct: 480 -MRLNDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRY 539

Query: 514 GAPPHGGFGVGLERVVMLFCGLNNIRKTSLFPRDP 536
           G   H GFG+G ER+++   GL+NIR    FPR P
Sbjct: 540 GTVKHCGFGLGFERMILFATGLDNIRDVIPFPRYP 567