MC11g0456 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g0456 vs. ExPASy Swiss-Prot
Match: Q94C48 (Protein WVD2-like 5 OS=Arabidopsis thaliana OX=3702 GN=WDL5 PE=1 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 3.1e-63
Identity = 187/419 (44.63%), Postives = 252/419 (60.14%), Query Frame = 0

Query: 66  TNESTTRELMEGSNFPAENNISTLSKATEFEGEENYVDPPKQVKSEKGQTKSKNEKSSGP 125
           T+ES        S      ++   ++ T+ E    +VD  K +K EK Q K ++EK SG 
Sbjct: 42  TSESQNENSANSSTLDTIEHVKEAAEGTQVE----HVDDSKCMKGEKAQRKPRHEKLSGG 101

Query: 126 KHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQ------- 185
           K+ SS  + K+K+GK A+   A  NGS   +  +  P KS+SFN R+AQ+ KQ       
Sbjct: 102 KNNSSVHIKKSKEGKSADAKVAASNGSVAPNVQTTNPLKSKSFNGREAQVTKQGKHDSAP 161

Query: 186 PEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRCNE 245
            E +DGE  K     K  KK     SE +++SS SP+A + KP +VG LPNYGFSF+C++
Sbjct: 162 AESADGEKVKP----KSQKKQAHETSEDDTQSSNSPKADDGKPRKVGALPNYGFSFKCDQ 221

Query: 246 RAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEPP 305
           RAEKRKEFY KLEEK HAKE E +++QAKSKETQEAE++MLRKSLNFKATPMPSFYQEP 
Sbjct: 222 RAEKRKEFYVKLEEKTHAKEEEINSMQAKSKETQEAELRMLRKSLNFKATPMPSFYQEPQ 281

Query: 306 PPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSKGV 365
           PPK ELKKIPPTR KSPKLGRKK   T + + S +    R GRLSLDE+AS +N ++KG+
Sbjct: 282 PPKTELKKIPPTRPKSPKLGRKK---TASGADSEETQTPRLGRLSLDERASKDNPTAKGI 341

Query: 366 SPA--RPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAP 425
            P     ++P R+SLPRLPS+KT++  G  A A        K+A   A    + E+K+  
Sbjct: 342 MPTVDLKKQPVRKSLPRLPSQKTVLPDGKPAPA--------KAAIIPAKV--RPEKKKLE 401

Query: 426 SDATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEE 476
            DA T       + +E ++         ++S +  ED  ET      E+++D+   + E
Sbjct: 402 KDAETVNQTSHPTEEEAQV--------TVSSNADVEDSHETVSPRMNEDRADKSIEVSE 431

BLAST of MC11g0456 vs. ExPASy Swiss-Prot
Match: Q0WSZ8 (Protein WVD2-like 6 OS=Arabidopsis thaliana OX=3702 GN=WDL6 PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 3.5e-54
Identity = 173/384 (45.05%), Postives = 220/384 (57.29%), Query Frame = 0

Query: 39  SEGINEDAGATMEQENMENSLKLSGSATNESTTRELMEGSNFPAENNISTLSKATEFEGE 98
           SE +    GA     N E +++   S  N  T+  L            ST       EG 
Sbjct: 3   SESVVAADGADCAIANGEVTMEGDSSNGNGGTSENL---------ECCSTQHPMEASEGT 62

Query: 99  EN-YVDPPKQVKSEKGQTKSKNEKSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHP 158
           +N  VD  KQ++ +K Q + K+EK+SG K+I S  V K KDGK         NGS   + 
Sbjct: 63  QNEQVDDSKQMRGQKVQGRVKHEKTSGGKNIPSVLVKKKKDGK-----VVASNGSVAPNV 122

Query: 159 HSKQPSKSRSFNDRQAQIPK--QPEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPR 218
              +  KS+S N R+A + K    +    EG+++   L+  +K     SE +++    P+
Sbjct: 123 KPVKSPKSKSLNGREAHVTKHGNHDSLPAEGTRDKPKLRETRKQVNDTSEDDTQ---YPK 182

Query: 219 AGEEKPHRVGRLPNYGFSFRCNERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAE 278
             + KP R   LPNYGFSFRC++RAEKR+EFYSKLEEKIHAKE EK+T+QAKSKETQEAE
Sbjct: 183 EDDGKPRRASALPNYGFSFRCDQRAEKRREFYSKLEEKIHAKEEEKNTVQAKSKETQEAE 242

Query: 279 IKMLRKSLNFKATPMPSFYQEPPPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGG 338
           +KMLRKSLNFKATPMP+FYQEP  PK ELKKI  TR KSPKLGRKK ++      +    
Sbjct: 243 LKMLRKSLNFKATPMPTFYQEPQLPKTELKKIAITRPKSPKLGRKKTNSRADSEEAITIQ 302

Query: 339 DVRSGRLSLDEKASLNNNSSKGVSPARPEKPK-RRSLPRLPSEKTMIASGAVASAGKSSA 398
             R GRLSLDEK   +N   +G  P   +KP  R+SLPRLPSEKT +++G VA A     
Sbjct: 303 TPRFGRLSLDEKTPKDNPVVEGSVPGETKKPPVRKSLPRLPSEKTNLSNGKVAPA----- 359

Query: 399 SKVKSAEKTASTNGKKEEKQAPSD 419
                   TAST  K E K+   D
Sbjct: 363 -----KAVTASTKAKSERKKPDKD 359

BLAST of MC11g0456 vs. ExPASy Swiss-Prot
Match: Q9SJ62 (Protein WVD2-like 4 OS=Arabidopsis thaliana OX=3702 GN=WDL4 PE=1 SV=2)

HSP 1 Score: 110.9 bits (276), Expect = 4.1e-23
Identity = 136/387 (35.14%), Postives = 181/387 (46.77%), Query Frame = 0

Query: 56  ENSLKLSGSATNESTTRELMEGSNFPAENNISTLSKATEFEGEEN-------YVD-PPKQ 115
           E  L  SG++    T ++     N P +    T S++  F  + +       Y D   K 
Sbjct: 58  EADLPESGTSVKSKTAKD-----NKPVKRKSGTFSRSPRFMSQSSSFPTKGAYTDITRKS 117

Query: 116 VKSEKGQTKSKNEKSSGPK----HISSTGVNKNKDG------KDAEQTAALLNGSGTSHP 175
           + +   +T  K   + G K      SS+GV+  +        K        ++    S P
Sbjct: 118 IDATTSKTSLKPVVAGGSKPKATPSSSSGVSAKRTSLVSAPLKKQTMPVKTISKDAASGP 177

Query: 176 HSKQPSKSRSFNDRQAQIPKQPEKSDGEGSKENTNLKPLKKGHPSKSE-----GESESSL 235
            SK        ++    I ++    D E +   T +   K  +P K+E      E   S 
Sbjct: 178 TSKLG------DEGSKSIKEETAGKDVEEAGSTTAVVADKVSNPLKAEMASKDDEDTRST 237

Query: 236 SPRAGEEKPHRVGRLPNYGFSFRCNERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQ 295
           +      +  R       GFSFR  ERAEKRKEFY KLEEKIHAKEVEK+ LQAKSKE+Q
Sbjct: 238 TTSTSTPRGRRSSVGSASGFSFRLEERAEKRKEFYMKLEEKIHAKEVEKTNLQAKSKESQ 297

Query: 296 EAEIKMLRKSLNFKATPMPSFYQEPPPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSN 355
           E EIK LRKSL FKA PMPSFY+E PPPK ELKKIP TR KSPKLGR+K       SS  
Sbjct: 298 EEEIKRLRKSLTFKAGPMPSFYKE-PPPKVELKKIPTTRPKSPKLGRRK------SSSDA 357

Query: 356 DGGDVRSGRLSLDEKASLNNNSSKGVSPARPEKPKRRSLPRLPS-EKTMIASGAVASAGK 415
            GG+           A+      K  S +  +KP  +S P+L + EK++ A        K
Sbjct: 358 TGGE-----------AAPRVTKPKDSSSSTVKKPITKSQPKLETQEKSVKAKEKKKKVKK 415

Query: 416 SSASKVKSAEKTASTNGKKEEKQAPSD 419
             A K    EK  +   K EE++  S+
Sbjct: 418 EEAEKRGEEEKATAVAAKPEEQKPNSN 415

BLAST of MC11g0456 vs. ExPASy Swiss-Prot
Match: Q84WL6 (Protein WVD2-like 3 OS=Arabidopsis thaliana OX=3702 GN=WDL3 PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 4.7e-19
Identity = 100/275 (36.36%), Postives = 136/275 (49.45%), Query Frame = 0

Query: 52  QENMENSLKLSGSATNESTTRELMEGSNFPAENNISTLSKATEFEGEENYVD-PPKQVKS 111
           QEN+   L  S S  + +   EL  G     E NI    +  E++ +E   + P  +   
Sbjct: 25  QENVSEPLLDSVSRDDANVHTELRYG-----EENI----EVNEYDVKECTSEIPVGKPIG 84

Query: 112 EKGQTKSKNEKSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFND 171
           +  ++K   + S   KH S +G   NK      Q  +L           K+ S +RSF  
Sbjct: 85  DDFESKDVTKSSLHAKHASKSGRGNNKTRNTVPQPFSLAT--------EKRASSTRSFTS 144

Query: 172 RQAQIPKQPEKSDGEGSKEN----TNLKPLKKGHPSKSEGESESSLSPRA-GEEKPHRVG 231
              +     +  DG    ++       KPL+  +   S+ E   S++  A    K  +  
Sbjct: 145 ESLESAGLKKFPDGHSKVQSQATKVPRKPLQPKNKKLSDEEDSCSVASYATSGAKSAKSR 204

Query: 232 RLPNYGFSFRCNERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNF 291
            +     SFR  ERAEKRKEFY+KLEEK  A E EK+  +A++KE  EA ++ LRKSL F
Sbjct: 205 TVVTAAPSFRSTERAEKRKEFYTKLEEKHQAMEAEKTQSEARNKEATEAALRQLRKSLRF 264

Query: 292 KATPMPSFYQEPPPPKAELKKIPPTRAKSPKLGRK 321
           KA PMP FY E PPPK ELKK  PTRAKSPKLGR+
Sbjct: 265 KANPMPKFYHEGPPPKVELKKPLPTRAKSPKLGRR 282

BLAST of MC11g0456 vs. ExPASy Swiss-Prot
Match: Q8GYX9 (Protein WVD2-like 1 OS=Arabidopsis thaliana OX=3702 GN=WDL1 PE=1 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 2.9e-16
Identity = 70/166 (42.17%), Postives = 96/166 (57.83%), Query Frame = 0

Query: 177 KQPEKSDGEG--SKENTNL----KPLKKGHPSKSEGESESSL-SPRAGEEKPHRVGRLPN 236
           K P+  +G    SK++  L    KPL+  +    + E   S+ S  A   +  + G    
Sbjct: 64  KSPKTRNGNAKVSKQDAPLLAVRKPLQPENKKHIDDEDNCSIASSVATSMRMGKSGLTYG 123

Query: 237 YGFSFRCNERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATP 296
              +FR  +RAEKRKE+Y KLEEK  A E E++ L+ + K+ QEA +K LRK+L FKA P
Sbjct: 124 SAPTFRSAQRAEKRKEYYQKLEEKNQALEAERNELEQRQKDEQEAALKQLRKNLKFKAKP 183

Query: 297 MPSFYQEPPPPKAELKKIPPTRAKSPK--LGRKKGSATLADSSSND 334
           +P+FY E PP K ELKK+P TR KSPK  L R+K  +    SSS +
Sbjct: 184 VPNFYYEAPPAKPELKKLPLTRPKSPKLILSRRKSFSDAVSSSSRE 229

BLAST of MC11g0456 vs. NCBI nr
Match: XP_022134503.1 (protein WVD2-like 5 isoform X3 [Momordica charantia])

HSP 1 Score: 871 bits (2251), Expect = 0.0
Identity = 497/503 (98.81%), Postives = 497/503 (98.81%), Query Frame = 0

Query: 1   MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK 60
           MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK
Sbjct: 1   MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK 60

Query: 61  LSGSATNESTTRELMEGSNFPAENNISTLSKATEFEGEENYVDPPKQVKSEKGQTKSKNE 120
           LSGSATNESTTRELMEGSNFPAENNISTLSK    EGEENYVDPPKQVKSEKGQTKSKNE
Sbjct: 61  LSGSATNESTTRELMEGSNFPAENNISTLSK----EGEENYVDPPKQVKSEKGQTKSKNE 120

Query: 121 KSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQ-- 180
           KSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQ  
Sbjct: 121 KSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQQQ 180

Query: 181 PEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRCNE 240
           PEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRCNE
Sbjct: 181 PEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRCNE 240

Query: 241 RAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEPP 300
           RAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEPP
Sbjct: 241 RAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEPP 300

Query: 301 PPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSKGV 360
           PPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSKGV
Sbjct: 301 PPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSKGV 360

Query: 361 SPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAPSD 420
           SPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAPSD
Sbjct: 361 SPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAPSD 420

Query: 421 ATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEEEHD 480
           ATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEEEHD
Sbjct: 421 ATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEEEHD 480

Query: 481 EQQQHKTLVEEGVEMEQSSSSRI 501
           EQQQHKTLVEEGVEMEQSSSSRI
Sbjct: 481 EQQQHKTLVEEGVEMEQSSSSRI 499

BLAST of MC11g0456 vs. NCBI nr
Match: XP_022134495.1 (protein WVD2-like 5 isoform X2 [Momordica charantia])

HSP 1 Score: 871 bits (2250), Expect = 0.0
Identity = 497/504 (98.61%), Postives = 497/504 (98.61%), Query Frame = 0

Query: 1   MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK 60
           MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK
Sbjct: 1   MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK 60

Query: 61  LSGSATNESTTRELMEGSNFPAENNISTLSKATEFEGEENYVDPPKQVKSEKGQTKSKNE 120
           LSGSATNESTTRELMEGSNFPAENNISTLSK    EGEENYVDPPKQVKSEKGQTKSKNE
Sbjct: 61  LSGSATNESTTRELMEGSNFPAENNISTLSK----EGEENYVDPPKQVKSEKGQTKSKNE 120

Query: 121 KSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQ-- 180
           KSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQ  
Sbjct: 121 KSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQQQ 180

Query: 181 -PEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRCN 240
            PEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRCN
Sbjct: 181 QPEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRCN 240

Query: 241 ERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEP 300
           ERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEP
Sbjct: 241 ERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEP 300

Query: 301 PPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSKG 360
           PPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSKG
Sbjct: 301 PPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSKG 360

Query: 361 VSPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAPS 420
           VSPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAPS
Sbjct: 361 VSPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAPS 420

Query: 421 DATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEEEH 480
           DATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEEEH
Sbjct: 421 DATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEEEH 480

Query: 481 DEQQQHKTLVEEGVEMEQSSSSRI 501
           DEQQQHKTLVEEGVEMEQSSSSRI
Sbjct: 481 DEQQQHKTLVEEGVEMEQSSSSRI 500

BLAST of MC11g0456 vs. NCBI nr
Match: XP_022134479.1 (protein WVD2-like 5 isoform X1 [Momordica charantia])

HSP 1 Score: 870 bits (2249), Expect = 0.0
Identity = 497/505 (98.42%), Postives = 497/505 (98.42%), Query Frame = 0

Query: 1   MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK 60
           MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK
Sbjct: 1   MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK 60

Query: 61  LSGSATNESTTRELMEGSNFPAENNISTLSKATEFEGEENYVDPPKQVKSEKGQTKSKNE 120
           LSGSATNESTTRELMEGSNFPAENNISTLSK    EGEENYVDPPKQVKSEKGQTKSKNE
Sbjct: 61  LSGSATNESTTRELMEGSNFPAENNISTLSK----EGEENYVDPPKQVKSEKGQTKSKNE 120

Query: 121 KSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQ-- 180
           KSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQ  
Sbjct: 121 KSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQQQ 180

Query: 181 --PEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRC 240
             PEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRC
Sbjct: 181 QQPEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRC 240

Query: 241 NERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQE 300
           NERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQE
Sbjct: 241 NERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQE 300

Query: 301 PPPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSK 360
           PPPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSK
Sbjct: 301 PPPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSK 360

Query: 361 GVSPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAP 420
           GVSPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAP
Sbjct: 361 GVSPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAP 420

Query: 421 SDATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEEE 480
           SDATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEEE
Sbjct: 421 SDATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEEE 480

Query: 481 HDEQQQHKTLVEEGVEMEQSSSSRI 501
           HDEQQQHKTLVEEGVEMEQSSSSRI
Sbjct: 481 HDEQQQHKTLVEEGVEMEQSSSSRI 501

BLAST of MC11g0456 vs. NCBI nr
Match: XP_038898383.1 (protein WVD2-like 5 [Benincasa hispida])

HSP 1 Score: 644 bits (1662), Expect = 2.17e-227
Identity = 395/508 (77.76%), Postives = 438/508 (86.22%), Query Frame = 0

Query: 1   MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK 60
           MMESEILVPA+GLKLTLQNGFHE VS SSE+IVP+VIVSEGI +D G TM+QEN+EN +K
Sbjct: 1   MMESEILVPADGLKLTLQNGFHEPVSGSSEEIVPEVIVSEGITKDTGDTMQQENIENDIK 60

Query: 61  LSGSATNESTTRELMEGSNFPAENNISTLSKATEFEGEENYVDPPKQVKSEKGQTKSKNE 120
           LSGSA NESTTREL EGS+F AE++ISTLSK    EGEE  VDPPK+VK EKGQ KSKNE
Sbjct: 61  LSGSAANESTTRELTEGSDFLAESDISTLSK----EGEEKNVDPPKKVKPEKGQIKSKNE 120

Query: 121 KSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGT--SHPHSKQPSKSRSFNDRQAQIPKQ 180
           KSSGPKHISSTG+NKNKDGKDAEQTAALLNGSGT  SHPH KQPSKSRSFN+RQ Q+PKQ
Sbjct: 121 KSSGPKHISSTGMNKNKDGKDAEQTAALLNGSGTGTSHPHPKQPSKSRSFNERQPQVPKQ 180

Query: 181 PEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRCNE 240
           PEKSDGEGSKENTNLKPLKKG PSKSEGESESS+SPRAG+EKP+RVGRLPNYGFSFRCNE
Sbjct: 181 PEKSDGEGSKENTNLKPLKKGQPSKSEGESESSISPRAGDEKPNRVGRLPNYGFSFRCNE 240

Query: 241 RAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEPP 300
           RAEKRKEFYSKLEEKI AKEVEK+TLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEPP
Sbjct: 241 RAEKRKEFYSKLEEKIQAKEVEKNTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEPP 300

Query: 301 PPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSKGV 360
           PPK ELKKIPPTRAKSPKLGRKK S+T ADSSSNDGGDVRS RLSLDE  +LNNNS KGV
Sbjct: 301 PPKVELKKIPPTRAKSPKLGRKK-SSTPADSSSNDGGDVRSARLSLDENVALNNNS-KGV 360

Query: 361 SPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTAS------TNGKKEE 420
            PAR EKPKRRSLP LPSE+T I  G V +AGKSSA+KVK+ EK A+      TNGKKEE
Sbjct: 361 YPARSEKPKRRSLPNLPSERTTIP-GVVTNAGKSSATKVKNVEKPAAAAAASTTNGKKEE 420

Query: 421 KQAPSDA-TTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEA 480
           K+  SDA TT+K+A  RS+ EEK A  +ATN+ + +LSQEE+GVE    SETENQ+D+E 
Sbjct: 421 KRVSSDAATTKKSASLRSI-EEKTAPSNATND-VVNLSQEENGVEP---SETENQTDDEP 480

Query: 481 VIEEEHDEQQQHKTLVEEGVEMEQSSSS 499
               E++EQ QH++  EEG E+EQSS++
Sbjct: 481 ----ENEEQDQHQSSAEEGAELEQSSNT 492

BLAST of MC11g0456 vs. NCBI nr
Match: XP_022940823.1 (protein WVD2-like 5 [Cucurbita moschata])

HSP 1 Score: 644 bits (1660), Expect = 5.62e-227
Identity = 396/508 (77.95%), Postives = 426/508 (83.86%), Query Frame = 0

Query: 1   MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK 60
           MMESEILVPA+GLKLTLQNGFH+H+SD+SEDIV KVIVSEGI+ D GAT +Q NMEN LK
Sbjct: 1   MMESEILVPADGLKLTLQNGFHDHISDNSEDIVAKVIVSEGISNDVGATTQQGNMENDLK 60

Query: 61  LSGSATNESTTRELMEGSNFPAENNISTLSKATEFEGEENYVDPPKQVKSEKGQTKSKNE 120
           LSGSATN+S TRELMEGS+FP E+NIS LSK    EG E  VDP KQVK EKGQ KSKNE
Sbjct: 61  LSGSATNDSKTRELMEGSSFPEESNISILSK----EGGEKNVDPSKQVKPEKGQIKSKNE 120

Query: 121 KSSGPKHISSTGVNKNKDGKDAEQTAALLNGS------GTSHPHSKQPSKSRSFNDRQAQ 180
           KSSGPKHI STGV KNK GKD+EQTAALLNGS      GTSHPHSKQ  KSRSFNDRQAQ
Sbjct: 121 KSSGPKHIHSTGVIKNKHGKDSEQTAALLNGSTSGTGTGTSHPHSKQHYKSRSFNDRQAQ 180

Query: 181 IPKQPEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSF 240
           +PKQ EKSDGEGSKENTNLKPL+KGHPSKSE ESESSLSPRAG+EKPHR+GRLPNYGFSF
Sbjct: 181 VPKQSEKSDGEGSKENTNLKPLQKGHPSKSEEESESSLSPRAGDEKPHRIGRLPNYGFSF 240

Query: 241 RCNERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFY 300
           RCNERAEKRKEFYSKLEEKI AKEVEK+TLQAKSKETQEAEIKM RKSLNFKATPMPSFY
Sbjct: 241 RCNERAEKRKEFYSKLEEKIQAKEVEKNTLQAKSKETQEAEIKMFRKSLNFKATPMPSFY 300

Query: 301 QEPPPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNS 360
           QEPPPPK ELKKIPPTRAKSPKLGRKKG  + AD+SSNDGGD RS RLSLDE A+L NNS
Sbjct: 301 QEPPPPKVELKKIPPTRAKSPKLGRKKG-LSPADTSSNDGGDFRSVRLSLDENAALINNS 360

Query: 361 SKGVSPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEK----TASTNGKK 420
            KGVSPAR EKPKRRSLPRLPSEK+ + SG +A  GK SA KVK+AEK     AST GKK
Sbjct: 361 -KGVSPARSEKPKRRSLPRLPSEKSAL-SGVIAHVGKPSAIKVKNAEKPAAAAASTIGKK 420

Query: 421 EEKQAPSDATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEE 480
           EEK+A SDA+TEK A  ++VDEEKIA  D TN+ +ASLSQEE+GV TAE SET   SDEE
Sbjct: 421 EEKRASSDASTEKPASLQTVDEEKIAPSDTTNKVVASLSQEENGVATAEPSETGINSDEE 480

Query: 481 AVIEEEHDEQQQHKTLVEEGVEMEQSSS 498
               EE  E  QH+T VEE VE EQ SS
Sbjct: 481 EAENEE--EYHQHQTSVEEAVESEQLSS 499

BLAST of MC11g0456 vs. ExPASy TrEMBL
Match: A0A6J1C259 (protein WVD2-like 5 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111006712 PE=3 SV=1)

HSP 1 Score: 871 bits (2251), Expect = 0.0
Identity = 497/503 (98.81%), Postives = 497/503 (98.81%), Query Frame = 0

Query: 1   MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK 60
           MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK
Sbjct: 1   MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK 60

Query: 61  LSGSATNESTTRELMEGSNFPAENNISTLSKATEFEGEENYVDPPKQVKSEKGQTKSKNE 120
           LSGSATNESTTRELMEGSNFPAENNISTLSK    EGEENYVDPPKQVKSEKGQTKSKNE
Sbjct: 61  LSGSATNESTTRELMEGSNFPAENNISTLSK----EGEENYVDPPKQVKSEKGQTKSKNE 120

Query: 121 KSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQ-- 180
           KSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQ  
Sbjct: 121 KSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQQQ 180

Query: 181 PEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRCNE 240
           PEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRCNE
Sbjct: 181 PEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRCNE 240

Query: 241 RAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEPP 300
           RAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEPP
Sbjct: 241 RAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEPP 300

Query: 301 PPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSKGV 360
           PPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSKGV
Sbjct: 301 PPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSKGV 360

Query: 361 SPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAPSD 420
           SPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAPSD
Sbjct: 361 SPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAPSD 420

Query: 421 ATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEEEHD 480
           ATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEEEHD
Sbjct: 421 ATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEEEHD 480

Query: 481 EQQQHKTLVEEGVEMEQSSSSRI 501
           EQQQHKTLVEEGVEMEQSSSSRI
Sbjct: 481 EQQQHKTLVEEGVEMEQSSSSRI 499

BLAST of MC11g0456 vs. ExPASy TrEMBL
Match: A0A6J1BYF9 (protein WVD2-like 5 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111006712 PE=3 SV=1)

HSP 1 Score: 871 bits (2250), Expect = 0.0
Identity = 497/504 (98.61%), Postives = 497/504 (98.61%), Query Frame = 0

Query: 1   MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK 60
           MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK
Sbjct: 1   MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK 60

Query: 61  LSGSATNESTTRELMEGSNFPAENNISTLSKATEFEGEENYVDPPKQVKSEKGQTKSKNE 120
           LSGSATNESTTRELMEGSNFPAENNISTLSK    EGEENYVDPPKQVKSEKGQTKSKNE
Sbjct: 61  LSGSATNESTTRELMEGSNFPAENNISTLSK----EGEENYVDPPKQVKSEKGQTKSKNE 120

Query: 121 KSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQ-- 180
           KSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQ  
Sbjct: 121 KSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQQQ 180

Query: 181 -PEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRCN 240
            PEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRCN
Sbjct: 181 QPEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRCN 240

Query: 241 ERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEP 300
           ERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEP
Sbjct: 241 ERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEP 300

Query: 301 PPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSKG 360
           PPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSKG
Sbjct: 301 PPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSKG 360

Query: 361 VSPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAPS 420
           VSPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAPS
Sbjct: 361 VSPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAPS 420

Query: 421 DATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEEEH 480
           DATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEEEH
Sbjct: 421 DATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEEEH 480

Query: 481 DEQQQHKTLVEEGVEMEQSSSSRI 501
           DEQQQHKTLVEEGVEMEQSSSSRI
Sbjct: 481 DEQQQHKTLVEEGVEMEQSSSSRI 500

BLAST of MC11g0456 vs. ExPASy TrEMBL
Match: A0A6J1BYU7 (protein WVD2-like 5 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111006712 PE=3 SV=1)

HSP 1 Score: 870 bits (2249), Expect = 0.0
Identity = 497/505 (98.42%), Postives = 497/505 (98.42%), Query Frame = 0

Query: 1   MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK 60
           MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK
Sbjct: 1   MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK 60

Query: 61  LSGSATNESTTRELMEGSNFPAENNISTLSKATEFEGEENYVDPPKQVKSEKGQTKSKNE 120
           LSGSATNESTTRELMEGSNFPAENNISTLSK    EGEENYVDPPKQVKSEKGQTKSKNE
Sbjct: 61  LSGSATNESTTRELMEGSNFPAENNISTLSK----EGEENYVDPPKQVKSEKGQTKSKNE 120

Query: 121 KSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQ-- 180
           KSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQ  
Sbjct: 121 KSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQQQ 180

Query: 181 --PEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRC 240
             PEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRC
Sbjct: 181 QQPEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRC 240

Query: 241 NERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQE 300
           NERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQE
Sbjct: 241 NERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQE 300

Query: 301 PPPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSK 360
           PPPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSK
Sbjct: 301 PPPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSK 360

Query: 361 GVSPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAP 420
           GVSPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAP
Sbjct: 361 GVSPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAP 420

Query: 421 SDATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEEE 480
           SDATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEEE
Sbjct: 421 SDATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEEE 480

Query: 481 HDEQQQHKTLVEEGVEMEQSSSSRI 501
           HDEQQQHKTLVEEGVEMEQSSSSRI
Sbjct: 481 HDEQQQHKTLVEEGVEMEQSSSSRI 501

BLAST of MC11g0456 vs. ExPASy TrEMBL
Match: A0A6J1FQD5 (protein WVD2-like 5 OS=Cucurbita moschata OX=3662 GN=LOC111446303 PE=3 SV=1)

HSP 1 Score: 644 bits (1660), Expect = 2.72e-227
Identity = 396/508 (77.95%), Postives = 426/508 (83.86%), Query Frame = 0

Query: 1   MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK 60
           MMESEILVPA+GLKLTLQNGFH+H+SD+SEDIV KVIVSEGI+ D GAT +Q NMEN LK
Sbjct: 1   MMESEILVPADGLKLTLQNGFHDHISDNSEDIVAKVIVSEGISNDVGATTQQGNMENDLK 60

Query: 61  LSGSATNESTTRELMEGSNFPAENNISTLSKATEFEGEENYVDPPKQVKSEKGQTKSKNE 120
           LSGSATN+S TRELMEGS+FP E+NIS LSK    EG E  VDP KQVK EKGQ KSKNE
Sbjct: 61  LSGSATNDSKTRELMEGSSFPEESNISILSK----EGGEKNVDPSKQVKPEKGQIKSKNE 120

Query: 121 KSSGPKHISSTGVNKNKDGKDAEQTAALLNGS------GTSHPHSKQPSKSRSFNDRQAQ 180
           KSSGPKHI STGV KNK GKD+EQTAALLNGS      GTSHPHSKQ  KSRSFNDRQAQ
Sbjct: 121 KSSGPKHIHSTGVIKNKHGKDSEQTAALLNGSTSGTGTGTSHPHSKQHYKSRSFNDRQAQ 180

Query: 181 IPKQPEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSF 240
           +PKQ EKSDGEGSKENTNLKPL+KGHPSKSE ESESSLSPRAG+EKPHR+GRLPNYGFSF
Sbjct: 181 VPKQSEKSDGEGSKENTNLKPLQKGHPSKSEEESESSLSPRAGDEKPHRIGRLPNYGFSF 240

Query: 241 RCNERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFY 300
           RCNERAEKRKEFYSKLEEKI AKEVEK+TLQAKSKETQEAEIKM RKSLNFKATPMPSFY
Sbjct: 241 RCNERAEKRKEFYSKLEEKIQAKEVEKNTLQAKSKETQEAEIKMFRKSLNFKATPMPSFY 300

Query: 301 QEPPPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNS 360
           QEPPPPK ELKKIPPTRAKSPKLGRKKG  + AD+SSNDGGD RS RLSLDE A+L NNS
Sbjct: 301 QEPPPPKVELKKIPPTRAKSPKLGRKKG-LSPADTSSNDGGDFRSVRLSLDENAALINNS 360

Query: 361 SKGVSPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEK----TASTNGKK 420
            KGVSPAR EKPKRRSLPRLPSEK+ + SG +A  GK SA KVK+AEK     AST GKK
Sbjct: 361 -KGVSPARSEKPKRRSLPRLPSEKSAL-SGVIAHVGKPSAIKVKNAEKPAAAAASTIGKK 420

Query: 421 EEKQAPSDATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEE 480
           EEK+A SDA+TEK A  ++VDEEKIA  D TN+ +ASLSQEE+GV TAE SET   SDEE
Sbjct: 421 EEKRASSDASTEKPASLQTVDEEKIAPSDTTNKVVASLSQEENGVATAEPSETGINSDEE 480

Query: 481 AVIEEEHDEQQQHKTLVEEGVEMEQSSS 498
               EE  E  QH+T VEE VE EQ SS
Sbjct: 481 EAENEE--EYHQHQTSVEEAVESEQLSS 499

BLAST of MC11g0456 vs. ExPASy TrEMBL
Match: A0A6J1ITP1 (protein WVD2-like 5 OS=Cucurbita maxima OX=3661 GN=LOC111480494 PE=3 SV=1)

HSP 1 Score: 636 bits (1640), Expect = 2.48e-224
Identity = 394/508 (77.56%), Postives = 424/508 (83.46%), Query Frame = 0

Query: 1   MMESEILVPAEGLKLTLQNGFHEHVSDSSEDIVPKVIVSEGINEDAGATMEQENMENSLK 60
           MMESEILVPA+GLKLTLQNGFH+H+SD+SEDIV KVIVSEGI+ D GAT +Q NMEN LK
Sbjct: 1   MMESEILVPADGLKLTLQNGFHDHISDNSEDIVAKVIVSEGISNDVGATTQQGNMENDLK 60

Query: 61  LSGSATNESTTRELMEGSNFPAENNISTLSKATEFEGEENYVDPPKQVKSEKGQTKSKNE 120
           LSGSATN+S TRELMEGS+FP E+NIS LSK    EG E  VDP KQVK EKGQ KSKNE
Sbjct: 61  LSGSATNDSKTRELMEGSSFPEESNISILSK----EGGEKNVDPSKQVKPEKGQIKSKNE 120

Query: 121 KSSGPKHISSTGVNKNKDGKDAEQTAALLNGS------GTSHPHSKQPSKSRSFNDRQAQ 180
           KSSGPKHI STGV KNK GKD+EQTAALLNGS      GTSHPHSKQ  KSRSFNDRQAQ
Sbjct: 121 KSSGPKHIHSTGVIKNKHGKDSEQTAALLNGSTSGTGTGTSHPHSKQHYKSRSFNDRQAQ 180

Query: 181 IPKQPEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSF 240
           +PKQ EKSDGEGSKENTNLKPL+KGHPSKSE ESESSLSPRAG+EKPHR+GRLPNYGFSF
Sbjct: 181 VPKQSEKSDGEGSKENTNLKPLQKGHPSKSEEESESSLSPRAGDEKPHRIGRLPNYGFSF 240

Query: 241 RCNERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFY 300
           RCNERAEKRKEFYSKLEEKI AKEVEK+TLQAKSKETQEAEIKM RKSLNFKATPMPSFY
Sbjct: 241 RCNERAEKRKEFYSKLEEKIQAKEVEKNTLQAKSKETQEAEIKMFRKSLNFKATPMPSFY 300

Query: 301 QEPPPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNS 360
           QEPPPPK ELKKIPPTRAKSPKLGRKKG  + AD+SSNDGGD RS RLSLDE A+L NNS
Sbjct: 301 QEPPPPKVELKKIPPTRAKSPKLGRKKG-LSPADTSSNDGGDFRSVRLSLDENAALINNS 360

Query: 361 SKGVSPARPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEK----TASTNGKK 420
            KGVSPAR EKPKRRSLPRLPSEK+ + SG +A AGKSSA KVK+AEK     AST GKK
Sbjct: 361 -KGVSPARSEKPKRRSLPRLPSEKSAL-SGVIAHAGKSSAIKVKNAEKPAAAAASTIGKK 420

Query: 421 EEKQAPSDATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEE 480
           EEK+A     +EK A  + VDEEKIA  D TN+ +ASLSQEE+GV TAE SET   SDEE
Sbjct: 421 EEKRA-----SEKPASLQMVDEEKIAPSDTTNKVVASLSQEENGVATAEPSETGINSDEE 480

Query: 481 AVIEEEHDEQQQHKTLVEEGVEMEQSSS 498
               EE  E  QH+T VEE VE+EQ SS
Sbjct: 481 EAENEE--EYHQHQTSVEEAVELEQPSS 494

BLAST of MC11g0456 vs. TAIR 10
Match: AT4G32330.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 244.2 bits (622), Expect = 2.2e-64
Identity = 187/419 (44.63%), Postives = 252/419 (60.14%), Query Frame = 0

Query: 66  TNESTTRELMEGSNFPAENNISTLSKATEFEGEENYVDPPKQVKSEKGQTKSKNEKSSGP 125
           T+ES        S      ++   ++ T+ E    +VD  K +K EK Q K ++EK SG 
Sbjct: 42  TSESQNENSANSSTLDTIEHVKEAAEGTQVE----HVDDSKCMKGEKAQRKPRHEKLSGG 101

Query: 126 KHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQ------- 185
           K+ SS  + K+K+GK A+   A  NGS   +  +  P KS+SFN R+AQ+ KQ       
Sbjct: 102 KNNSSVHIKKSKEGKSADAKVAASNGSVAPNVQTTNPLKSKSFNGREAQVTKQGKHDSAP 161

Query: 186 PEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRCNE 245
            E +DGE  K     K  KK     SE +++SS SP+A + KP +VG LPNYGFSF+C++
Sbjct: 162 AESADGEKVKP----KSQKKQAHETSEDDTQSSNSPKADDGKPRKVGALPNYGFSFKCDQ 221

Query: 246 RAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEPP 305
           RAEKRKEFY KLEEK HAKE E +++QAKSKETQEAE++MLRKSLNFKATPMPSFYQEP 
Sbjct: 222 RAEKRKEFYVKLEEKTHAKEEEINSMQAKSKETQEAELRMLRKSLNFKATPMPSFYQEPQ 281

Query: 306 PPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSKGV 365
           PPK ELKKIPPTR KSPKLGRKK   T + + S +    R GRLSLDE+AS +N ++KG+
Sbjct: 282 PPKTELKKIPPTRPKSPKLGRKK---TASGADSEETQTPRLGRLSLDERASKDNPTAKGI 341

Query: 366 SPA--RPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAP 425
            P     ++P R+SLPRLPS+KT++  G  A A        K+A   A    + E+K+  
Sbjct: 342 MPTVDLKKQPVRKSLPRLPSQKTVLPDGKPAPA--------KAAIIPAKV--RPEKKKLE 401

Query: 426 SDATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEE 476
            DA T       + +E ++         ++S +  ED  ET      E+++D+   + E
Sbjct: 402 KDAETVNQTSHPTEEEAQV--------TVSSNADVEDSHETVSPRMNEDRADKSIEVSE 431

BLAST of MC11g0456 vs. TAIR 10
Match: AT4G32330.3 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 244.2 bits (622), Expect = 2.2e-64
Identity = 187/419 (44.63%), Postives = 252/419 (60.14%), Query Frame = 0

Query: 66  TNESTTRELMEGSNFPAENNISTLSKATEFEGEENYVDPPKQVKSEKGQTKSKNEKSSGP 125
           T+ES        S      ++   ++ T+ E    +VD  K +K EK Q K ++EK SG 
Sbjct: 42  TSESQNENSANSSTLDTIEHVKEAAEGTQVE----HVDDSKCMKGEKAQRKPRHEKLSGG 101

Query: 126 KHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQ------- 185
           K+ SS  + K+K+GK A+   A  NGS   +  +  P KS+SFN R+AQ+ KQ       
Sbjct: 102 KNNSSVHIKKSKEGKSADAKVAASNGSVAPNVQTTNPLKSKSFNGREAQVTKQGKHDSAP 161

Query: 186 PEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRCNE 245
            E +DGE  K     K  KK     SE +++SS SP+A + KP +VG LPNYGFSF+C++
Sbjct: 162 AESADGEKVKP----KSQKKQAHETSEDDTQSSNSPKADDGKPRKVGALPNYGFSFKCDQ 221

Query: 246 RAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEPP 305
           RAEKRKEFY KLEEK HAKE E +++QAKSKETQEAE++MLRKSLNFKATPMPSFYQEP 
Sbjct: 222 RAEKRKEFYVKLEEKTHAKEEEINSMQAKSKETQEAELRMLRKSLNFKATPMPSFYQEPQ 281

Query: 306 PPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSKGV 365
           PPK ELKKIPPTR KSPKLGRKK   T + + S +    R GRLSLDE+AS +N ++KG+
Sbjct: 282 PPKTELKKIPPTRPKSPKLGRKK---TASGADSEETQTPRLGRLSLDERASKDNPTAKGI 341

Query: 366 SPA--RPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAP 425
            P     ++P R+SLPRLPS+KT++  G  A A        K+A   A    + E+K+  
Sbjct: 342 MPTVDLKKQPVRKSLPRLPSQKTVLPDGKPAPA--------KAAIIPAKV--RPEKKKLE 401

Query: 426 SDATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEE 476
            DA T       + +E ++         ++S +  ED  ET      E+++D+   + E
Sbjct: 402 KDAETVNQTSHPTEEEAQV--------TVSSNADVEDSHETVSPRMNEDRADKSIEVSE 431

BLAST of MC11g0456 vs. TAIR 10
Match: AT4G32330.2 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 239.2 bits (609), Expect = 7.2e-63
Identity = 186/419 (44.39%), Postives = 252/419 (60.14%), Query Frame = 0

Query: 66  TNESTTRELMEGSNFPAENNISTLSKATEFEGEENYVDPPKQVKSEKGQTKSKNEKSSGP 125
           T+ES        S      ++   ++ T+ E    +VD  K +K EK Q K ++EK SG 
Sbjct: 42  TSESQNENSANSSTLDTIEHVKEAAEGTQVE----HVDDSKCMKGEKAQRKPRHEKLSGG 101

Query: 126 KHISSTGVNKNKDGKDAEQTAALLNGSGTSHPHSKQPSKSRSFNDRQAQIPKQ------- 185
           K+ SS  + K+K+GK A+   A  NGS   +  +  P KS+SFN R+AQ+ KQ       
Sbjct: 102 KNNSSVHIKKSKEGKSADAKVAASNGSVAPNVQTTNPLKSKSFNGREAQVTKQGKHDSAP 161

Query: 186 PEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPRAGEEKPHRVGRLPNYGFSFRCNE 245
            E +DGE  K     K  KK     SE +++SS +P+A + KP +VG LPNYGFSF+C++
Sbjct: 162 AESADGEKVKP----KSQKKQAHETSEDDTQSS-NPKADDGKPRKVGALPNYGFSFKCDQ 221

Query: 246 RAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAEIKMLRKSLNFKATPMPSFYQEPP 305
           RAEKRKEFY KLEEK HAKE E +++QAKSKETQEAE++MLRKSLNFKATPMPSFYQEP 
Sbjct: 222 RAEKRKEFYVKLEEKTHAKEEEINSMQAKSKETQEAELRMLRKSLNFKATPMPSFYQEPQ 281

Query: 306 PPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGGDVRSGRLSLDEKASLNNNSSKGV 365
           PPK ELKKIPPTR KSPKLGRKK   T + + S +    R GRLSLDE+AS +N ++KG+
Sbjct: 282 PPKTELKKIPPTRPKSPKLGRKK---TASGADSEETQTPRLGRLSLDERASKDNPTAKGI 341

Query: 366 SPA--RPEKPKRRSLPRLPSEKTMIASGAVASAGKSSASKVKSAEKTASTNGKKEEKQAP 425
            P     ++P R+SLPRLPS+KT++  G  A A        K+A   A    + E+K+  
Sbjct: 342 MPTVDLKKQPVRKSLPRLPSQKTVLPDGKPAPA--------KAAIIPAKV--RPEKKKLE 401

Query: 426 SDATTEKTACSRSVDEEKIASLDATNEALASLSQEEDGVETAEASETENQSDEEAVIEE 476
            DA T       + +E ++         ++S +  ED  ET      E+++D+   + E
Sbjct: 402 KDAETVNQTSHPTEEEAQV--------TVSSNADVEDSHETVSPRMNEDRADKSIEVSE 430

BLAST of MC11g0456 vs. TAIR 10
Match: AT2G25480.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 214.2 bits (544), Expect = 2.5e-55
Identity = 173/384 (45.05%), Postives = 220/384 (57.29%), Query Frame = 0

Query: 39  SEGINEDAGATMEQENMENSLKLSGSATNESTTRELMEGSNFPAENNISTLSKATEFEGE 98
           SE +    GA     N E +++   S  N  T+  L            ST       EG 
Sbjct: 3   SESVVAADGADCAIANGEVTMEGDSSNGNGGTSENL---------ECCSTQHPMEASEGT 62

Query: 99  EN-YVDPPKQVKSEKGQTKSKNEKSSGPKHISSTGVNKNKDGKDAEQTAALLNGSGTSHP 158
           +N  VD  KQ++ +K Q + K+EK+SG K+I S  V K KDGK         NGS   + 
Sbjct: 63  QNEQVDDSKQMRGQKVQGRVKHEKTSGGKNIPSVLVKKKKDGK-----VVASNGSVAPNV 122

Query: 159 HSKQPSKSRSFNDRQAQIPK--QPEKSDGEGSKENTNLKPLKKGHPSKSEGESESSLSPR 218
              +  KS+S N R+A + K    +    EG+++   L+  +K     SE +++    P+
Sbjct: 123 KPVKSPKSKSLNGREAHVTKHGNHDSLPAEGTRDKPKLRETRKQVNDTSEDDTQ---YPK 182

Query: 219 AGEEKPHRVGRLPNYGFSFRCNERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQEAE 278
             + KP R   LPNYGFSFRC++RAEKR+EFYSKLEEKIHAKE EK+T+QAKSKETQEAE
Sbjct: 183 EDDGKPRRASALPNYGFSFRCDQRAEKRREFYSKLEEKIHAKEEEKNTVQAKSKETQEAE 242

Query: 279 IKMLRKSLNFKATPMPSFYQEPPPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSNDGG 338
           +KMLRKSLNFKATPMP+FYQEP  PK ELKKI  TR KSPKLGRKK ++      +    
Sbjct: 243 LKMLRKSLNFKATPMPTFYQEPQLPKTELKKIAITRPKSPKLGRKKTNSRADSEEAITIQ 302

Query: 339 DVRSGRLSLDEKASLNNNSSKGVSPARPEKPK-RRSLPRLPSEKTMIASGAVASAGKSSA 398
             R GRLSLDEK   +N   +G  P   +KP  R+SLPRLPSEKT +++G VA A     
Sbjct: 303 TPRFGRLSLDEKTPKDNPVVEGSVPGETKKPPVRKSLPRLPSEKTNLSNGKVAPA----- 359

Query: 399 SKVKSAEKTASTNGKKEEKQAPSD 419
                   TAST  K E K+   D
Sbjct: 363 -----KAVTASTKAKSERKKPDKD 359

BLAST of MC11g0456 vs. TAIR 10
Match: AT2G35880.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 110.9 bits (276), Expect = 2.9e-24
Identity = 136/387 (35.14%), Postives = 181/387 (46.77%), Query Frame = 0

Query: 56  ENSLKLSGSATNESTTRELMEGSNFPAENNISTLSKATEFEGEEN-------YVD-PPKQ 115
           E  L  SG++    T ++     N P +    T S++  F  + +       Y D   K 
Sbjct: 58  EADLPESGTSVKSKTAKD-----NKPVKRKSGTFSRSPRFMSQSSSFPTKGAYTDITRKS 117

Query: 116 VKSEKGQTKSKNEKSSGPK----HISSTGVNKNKDG------KDAEQTAALLNGSGTSHP 175
           + +   +T  K   + G K      SS+GV+  +        K        ++    S P
Sbjct: 118 IDATTSKTSLKPVVAGGSKPKATPSSSSGVSAKRTSLVSAPLKKQTMPVKTISKDAASGP 177

Query: 176 HSKQPSKSRSFNDRQAQIPKQPEKSDGEGSKENTNLKPLKKGHPSKSE-----GESESSL 235
            SK        ++    I ++    D E +   T +   K  +P K+E      E   S 
Sbjct: 178 TSKLG------DEGSKSIKEETAGKDVEEAGSTTAVVADKVSNPLKAEMASKDDEDTRST 237

Query: 236 SPRAGEEKPHRVGRLPNYGFSFRCNERAEKRKEFYSKLEEKIHAKEVEKSTLQAKSKETQ 295
           +      +  R       GFSFR  ERAEKRKEFY KLEEKIHAKEVEK+ LQAKSKE+Q
Sbjct: 238 TTSTSTPRGRRSSVGSASGFSFRLEERAEKRKEFYMKLEEKIHAKEVEKTNLQAKSKESQ 297

Query: 296 EAEIKMLRKSLNFKATPMPSFYQEPPPPKAELKKIPPTRAKSPKLGRKKGSATLADSSSN 355
           E EIK LRKSL FKA PMPSFY+E PPPK ELKKIP TR KSPKLGR+K       SS  
Sbjct: 298 EEEIKRLRKSLTFKAGPMPSFYKE-PPPKVELKKIPTTRPKSPKLGRRK------SSSDA 357

Query: 356 DGGDVRSGRLSLDEKASLNNNSSKGVSPARPEKPKRRSLPRLPS-EKTMIASGAVASAGK 415
            GG+           A+      K  S +  +KP  +S P+L + EK++ A        K
Sbjct: 358 TGGE-----------AAPRVTKPKDSSSSTVKKPITKSQPKLETQEKSVKAKEKKKKVKK 415

Query: 416 SSASKVKSAEKTASTNGKKEEKQAPSD 419
             A K    EK  +   K EE++  S+
Sbjct: 418 EEAEKRGEEEKATAVAAKPEEQKPNSN 415