MC11g0239 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g0239
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	heat stress transcription factor B-4-like
Location	MC11: 1887096 .. 1889128 (-)
RNA-Seq Expression	MC11g0239
Synteny	MC11g0239

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CGCACAGGCTGCACCACTGCACCATATATAACCTCTTGTGGTGATTTTTAAGTGGGTGCGCGAGGGATGGATGTCAGTAGTGAAAGAGAGAGAGAGAAAAAAAAGAAAGAGAGATGGACATGTCACGGGTTTGTCTTGAAAGGCCCTTATTTCACAGTCCTAGACAGACAGACACATATTCTCTCTCACCACAAAACCGAGGACCCAAAAACCAAACCTTTTTTTAATATAAAAAGAAAAACTAACTGCACGACACGACACACTTGCTTTTCCTTTGTTTTTGCTTTCTTTTATTGCTTCTCTCTCTTAAACATTTTATCCACCAGAGTCTCACTCGCTATCTCTATCTCTATCTCATCTGCAGCTCCCCAAAAAGACTGTTCCTTTCTCTTTCTCTTTCTCTTTCTCTGTGGCTGCTGCTTCATTCAGATTCAGCCATTGAAATGGCTCTAATGCTCGATAACTGCGAAGGGGTTCTACTGTCCCTAGACTCCCACAAGGCAATCCCCGCCCCATTCCTCACTAAAACTTACCAGCTCGTCGACGATCCCACCACTGACCACATCGTCTCATGGGGCGAAGACGACACCACTTTCGTCGTTTGGCGCCCTCCTGAATTCGCCAGAGATCTCCTCCCTAATTACTTCAAGCACAACAACTTCTCCAGCTTCGTCCGCCAGCTCAACACCTATGTAAGCACAAATCAATTATCCACAATTTAACAAAACCCTTCTTTGTAATTTCATAACAAAAATTGATTCTTTTTTTTTTTTTGGGTAATTAGGGTTTTAGAAAAATTGTGCCGGACAGGTGGGAGTTCGCCAACGAGTTCTTCAGGAAAGGAGAGAAACATTTATTATGCGAGATCCATAGACGGAAAACAGCTCAGCCGCCACAACTCACCGTCAACCAACACCACCACCATTCTCCACTCGGCATCAACGCTCCGAGTTACTACCACTTCCCCGGCCGACTCAGCATCTCGCCGTCCGAGTCAGACGACCAACCCAATTGGTGTGACTCGCCGCCGCTCTCCTCCCCGACAGGCGGTGGCAATAACAACAACAATAACAACAACAACTCAGTAACCGCGCTGTCGGAGGACAACGAGCGGCTGAGGCGGAGTAACAACATGCTGATGTCGGAACTCGCCCACATGAAAAAGCTCTACAACGACATCATCTATTTCGTTCAGAACCACGTCAAGCCCGTGGCTCCGAGCAACTCATACCAATACTCCACCACCTCATTACTCTCCGACGGCTTTCCGGCGCGGCCTCCTCCGAGCCACCTTCTCGGGTTCCACCCCACCAACCCAAAGCCGCCGGTTTCCGGTCACGCCCATTTTCCCACCGCTCCAAATAACAACAACGCCCCCTCCAGAAGCAGCTTCGTGACCATTCTCGACGAACCGCCGGCGGCGCCCACCTGCAAAACCAAGCTATTCGGGGTCCCGCTTCAATCGAAGAAAAGGCTGCACCCGGAATATAGTTCCAATTCTAATATTCAAATCGGCGGCAAAGACAACAACAAGGCTCGATTGGTGTTGGAGAAAGATGATTTAGGGCTCAATCTCATGCCTCCTTCCGCATGTTAGTTTTTAATCCAATTCCCTTTTCACCCCAAAAAAAAAAAACTTATTTATTAAAAAAAGAAAAAAATTAGTCTTCCCATTTTTACTTCTTACAAATCTCAGAGATCCTTCCCTTCCCAGTGCATTATTCACATCATTGAATATGTGTGCGTGCGTATGGTTGTTTGTGATGATAGTGTAAGTTTTTTTTTCTTTTCTTTTTTTCCTTTTTTAATGTATATAACCCTCAGTGACGGTATTGAGTCTCTGTTCCCCATGACCATTATTGTTGCAGTGGCCTCTCTCTCTCTCTCTCTGGTTGTAGAGAAGAAGAAACAGGAGTTTTAAAAAATGACTATCTTTTAGTTCTAATTCCCTAAAGGGTTTTCCTTTTTTCTCTTTCCCTTTCCATTATTTGTTCCAATTAAATTCCATCTTTTTTTATATATCTCTCTCATAT

mRNA sequence

CGCACAGGCTGCACCACTGCACCATATATAACCTCTTGTGGTGATTTTTAAGTGGGTGCGCGAGGGATGGATGTCAGTAGTGAAAGAGAGAGAGAGAAAAAAAAGAAAGAGAGATGGACATGTCACGGCTCCCCAAAAAGACTGTTCCTTTCTCTTTCTCTTTCTCTTTCTCTGTGGCTGCTGCTTCATTCAGATTCAGCCATTGAAATGGCTCTAATGCTCGATAACTGCGAAGGGGTTCTACTGTCCCTAGACTCCCACAAGGCAATCCCCGCCCCATTCCTCACTAAAACTTACCAGCTCGTCGACGATCCCACCACTGACCACATCGTCTCATGGGGCGAAGACGACACCACTTTCGTCGTTTGGCGCCCTCCTGAATTCGCCAGAGATCTCCTCCCTAATTACTTCAAGCACAACAACTTCTCCAGCTTCGTCCGCCAGCTCAACACCTATGGTTTTAGAAAAATTGTGCCGGACAGGTGGGAGTTCGCCAACGAGTTCTTCAGGAAAGGAGAGAAACATTTATTATGCGAGATCCATAGACGGAAAACAGCTCAGCCGCCACAACTCACCGTCAACCAACACCACCACCATTCTCCACTCGGCATCAACGCTCCGAGTTACTACCACTTCCCCGGCCGACTCAGCATCTCGCCGTCCGAGTCAGACGACCAACCCAATTGGTGTGACTCGCCGCCGCTCTCCTCCCCGACAGGCGGTGGCAATAACAACAACAATAACAACAACAACTCAGTAACCGCGCTGTCGGAGGACAACGAGCGGCTGAGGCGGAGTAACAACATGCTGATGTCGGAACTCGCCCACATGAAAAAGCTCTACAACGACATCATCTATTTCGTTCAGAACCACGTCAAGCCCGTGGCTCCGAGCAACTCATACCAATACTCCACCACCTCATTACTCTCCGACGGCTTTCCGGCGCGGCCTCCTCCGAGCCACCTTCTCGGGTTCCACCCCACCAACCCAAAGCCGCCGGTTTCCGGTCACGCCCATTTTCCCACCGCTCCAAATAACAACAACGCCCCCTCCAGAAGCAGCTTCGTGACCATTCTCGACGAACCGCCGGCGGCGCCCACCTGCAAAACCAAGCTATTCGGGGTCCCGCTTCAATCGAAGAAAAGGCTGCACCCGGAATATAGTTCCAATTCTAATATTCAAATCGGCGGCAAAGACAACAACAAGGCTCGATTGGTGTTGGAGAAAGATGATTTAGGGCTCAATCTCATGCCTCCTTCCGCATGTTAGTTTTTAATCCAATTCCCTTTTCACCCCAAAAAAAAAAAACTTATTTATTAAAAAAAGAAAAAAATTAGTCTTCCCATTTTTACTTCTTACAAATCTCAGAGATCCTTCCCTTCCCAGTGCATTATTCACATCATTGAATATGTGTGCGTGCGTATGGTTGTTTGTGATGATAGTGTAAGTTTTTTTTTCTTTTCTTTTTTTCCTTTTTTAATGTATATAACCCTCAGTGACGGTATTGAGTCTCTGTTCCCCATGACCATTATTGTTGCAGTGGCCTCTCTCTCTCTCTCTCTGGTTGTAGAGAAGAAGAAACAGGAGTTTTAAAAAATGACTATCTTTTAGTTCTAATTCCCTAAAGGGTTTTCCTTTTTTCTCTTTCCCTTTCCATTATTTGTTCCAATTAAATTCCATCTTTTTTTATATATCTCTCTCATAT

Coding sequence (CDS)

ATGGATGTCAGTAGTGAAAGAGAGAGAGAGAAAAAAAAGAAAGAGAGATGGACATGTCACGGCTCCCCAAAAAGACTGTTCCTTTCTCTTTCTCTTTCTCTTTCTCTGTGGCTGCTGCTTCATTCAGATTCAGCCATTGAAATGGCTCTAATGCTCGATAACTGCGAAGGGGTTCTACTGTCCCTAGACTCCCACAAGGCAATCCCCGCCCCATTCCTCACTAAAACTTACCAGCTCGTCGACGATCCCACCACTGACCACATCGTCTCATGGGGCGAAGACGACACCACTTTCGTCGTTTGGCGCCCTCCTGAATTCGCCAGAGATCTCCTCCCTAATTACTTCAAGCACAACAACTTCTCCAGCTTCGTCCGCCAGCTCAACACCTATGGTTTTAGAAAAATTGTGCCGGACAGGTGGGAGTTCGCCAACGAGTTCTTCAGGAAAGGAGAGAAACATTTATTATGCGAGATCCATAGACGGAAAACAGCTCAGCCGCCACAACTCACCGTCAACCAACACCACCACCATTCTCCACTCGGCATCAACGCTCCGAGTTACTACCACTTCCCCGGCCGACTCAGCATCTCGCCGTCCGAGTCAGACGACCAACCCAATTGGTGTGACTCGCCGCCGCTCTCCTCCCCGACAGGCGGTGGCAATAACAACAACAATAACAACAACAACTCAGTAACCGCGCTGTCGGAGGACAACGAGCGGCTGAGGCGGAGTAACAACATGCTGATGTCGGAACTCGCCCACATGAAAAAGCTCTACAACGACATCATCTATTTCGTTCAGAACCACGTCAAGCCCGTGGCTCCGAGCAACTCATACCAATACTCCACCACCTCATTACTCTCCGACGGCTTTCCGGCGCGGCCTCCTCCGAGCCACCTTCTCGGGTTCCACCCCACCAACCCAAAGCCGCCGGTTTCCGGTCACGCCCATTTTCCCACCGCTCCAAATAACAACAACGCCCCCTCCAGAAGCAGCTTCGTGACCATTCTCGACGAACCGCCGGCGGCGCCCACCTGCAAAACCAAGCTATTCGGGGTCCCGCTTCAATCGAAGAAAAGGCTGCACCCGGAATATAGTTCCAATTCTAATATTCAAATCGGCGGCAAAGACAACAACAAGGCTCGATTGGTGTTGGAGAAAGATGATTTAGGGCTCAATCTCATGCCTCCTTCCGCATGTTAG

Protein sequence

MDVSSEREREKKKKERWTCHGSPKRLFLSLSLSLSLWLLLHSDSAIEMALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPPQLTVNQHHHHSPLGINAPSYYHFPGRLSISPSESDDQPNWCDSPPLSSPTGGGNNNNNNNNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSLLSDGFPARPPPSHLLGFHPTNPKPPVSGHAHFPTAPNNNNAPSRSSFVTILDEPPAAPTCKTKLFGVPLQSKKRLHPEYSSNSNIQIGGKDNNKARLVLEKDDLGLNLMPPSAC

Homology

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BLAST of MC11g0239 vs. ExPASy Swiss-Prot
Match: Q9C635 (Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE=2 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 4.2e-87
Identity = 196/370 (52.97%), Postives = 236/370 (63.78%), Query Frame = 0


Query: 48  MALMLDNCEG----------VLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTT 107
           MA+M++N  G           L+     KA+PAPFLTKTYQLVDDP TDH+VSWG+DDTT
Sbjct: 1   MAMMVENSYGGYGGGGGERIQLMVEGQGKAVPAPFLTKTYQLVDDPATDHVVSWGDDDTT 60

Query: 108 FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCE 167
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFF++GEKHLLCE
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFKRGEKHLLCE 120

Query: 168 IHRRKTAQ--PPQLTVNQHHHHSP--LGINAPSYYHFPGRLSISPSESDDQPNWCDSPPL 227
           IHRRKT+Q  P Q +    HHH+P  +  +  S++  P     +P E      WCD  P 
Sbjct: 121 IHRRKTSQMIPQQHSPFMSHHHAPPQIPFSGGSFFPLPPPRVTTPEEDH---YWCDDSPP 180

Query: 228 SSPTGGGNNNNNNNNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPV 287
           S P         +    VTALSEDNERLRRSN +LMSELAHMKKLYNDIIYFVQNHVKPV
Sbjct: 181 SRPR--VIPQQIDTAAQVTALSEDNERLRRSNTVLMSELAHMKKLYNDIIYFVQNHVKPV 240

Query: 288 APSNSYQYSTTSLLSDGFPARPPPSHLLGFHPTNPKPPVSGHAHFPTAPNNNNAPSRSSF 347
           APSN+  Y  +S L      +PP    L ++ T      + +A       N++ P+  S 
Sbjct: 241 APSNNSSY-LSSFLQKQQQQQPP---TLDYYNTATVNATNLNA------LNSSPPTSQSS 300

Query: 348 VTILDEP-----PAAPTCKTKLFGVPLQSKKRLHPEYSSNSNIQIGGKDNNKARLVLEKD 399
           +T+L++        +   KTKLFGV L S K+    +S  +         +K RLVL++ 
Sbjct: 301 ITVLEDDHTNHHDQSNMRKTKLFGVSLPSSKKRSHHFSDQT---------SKTRLVLDQS 346

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BLAST of MC11g0239 vs. ExPASy Swiss-Prot
Match: Q7XHZ0 (Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4B PE=1 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 5.8e-68
Identity = 157/333 (47.15%), Postives = 192/333 (57.66%), Query Frame = 0


Query: 48  MALMLDNCEGVLLSLD----------SHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTT 107
           MA +++ C  +++S++          + K +PAPFLTKTYQLVDDP TDHIVSWGEDDTT
Sbjct: 1   MAFLVERCGEMVVSMEMGPHGGGGAAAGKPVPAPFLTKTYQLVDDPCTDHIVSWGEDDTT 60

Query: 108 FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCE 167
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIV DRWEFANEFFRKG KHLL E
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVADRWEFANEFFRKGAKHLLAE 120

Query: 168 IHRRKTAQP-----PQLTVNQHHHHSPLGI--NAPSYYHFPGRLSISPSESDDQPNWCDS 227
           IHRRK++QP     P    + HHH +P  +    P+Y+H                +    
Sbjct: 121 IHRRKSSQPPPPPMPHQPYHHHHHLNPFSLPPPPPAYHHH---------------HLIQE 180

Query: 228 PPLSSPTGGGNNNNNNNNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHV 287
            P ++       +     + + ALSEDN +LRR N++L+SELAHMKKLYNDIIYF+QNHV
Sbjct: 181 EPATTAHCTVAGDGGEGGDFLAALSEDNRQLRRRNSLLLSELAHMKKLYNDIIYFLQNHV 240

Query: 288 KPVAPSNSYQYSTTSLLSDGFPARPPPSHLLGFHPTNPKPPVSGHAHFPTAPNNNNAPSR 347
            PV  + +   ST    +           L+     +  PP          P     P+ 
Sbjct: 241 APVTTTTTTPSSTAMAAAQHHLPAAASCRLMELDSPDHSPP----------PPPPKTPAT 296

Query: 348 SSFVTILDEPPAAPTCKTKLFGVPLQS-KKRLH 363
               T+            KLFGV L   KKR H
Sbjct: 301 DGGDTV------------KLFGVSLHGRKKRAH 296

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BLAST of MC11g0239 vs. ExPASy Swiss-Prot
Match: Q67U94 (Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4C PE=2 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 5.8e-68
Identity = 180/405 (44.44%), Postives = 216/405 (53.33%), Query Frame = 0


Query: 54  NCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDD-TTFVVWRPPEFARDLLP 113
           +CE    +  + KA+PAPFLTKTYQLVDDP TDHIVSWG+D  +TFVVWRPPEFARD+LP
Sbjct: 9   DCEAAAAA--AQKAVPAPFLTKTYQLVDDPATDHIVSWGDDRVSTFVVWRPPEFARDILP 68

Query: 114 NYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTA-------Q 173
           NYFKHNNFSSFVRQLNTYGFRK+VP+RWEFANEFFRKGEK LL EIHRRKT+        
Sbjct: 69  NYFKHNNFSSFVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLTEIHRRKTSSASTASPS 128

Query: 174 PPQLTVNQHH--HHSPLGINAPSYYH-FPGRLSISPSES----DDQPNWCD--------- 233
           PP      H    H P G+ A  ++H F G   +  +        QP+W +         
Sbjct: 129 PPPFFAPPHFPLFHHP-GVAAAQHHHAFVGDDGVVAAHGIGMPFPQPHWREPNLPVATRL 188

Query: 234 ------SPPLSSPTGGGNNNNNNNNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDII 293
                 +P  SS   GG         +   L E+NERLRRSN  L+ ELAHM+KLYNDII
Sbjct: 189 LALGGPAPSPSSAEAGG----AGRAATAAVLMEENERLRRSNTALLQELAHMRKLYNDII 248

Query: 294 YFVQNHVKPVAPSNSYQYSTTSLLSDGFPARPPPSHLLGFHPTNPKPPVSGHAHFPTAPN 353
           YFVQNHV+PVAPS +   +   L   G  AR  P                  A      N
Sbjct: 249 YFVQNHVRPVAPSPA---AAAFLQGLGMQARKKP------------------AAANVLNN 308

Query: 354 NNNAPSRSSFVTILDEPPAAP----------------------TCKTKLFGVPLQ----- 400
           +  + + SS +TI +EP   P                      +  TKLFGV L      
Sbjct: 309 SGGSTTSSSSLTIAEEPSPPPQQQHLAGEKSGGEAGNSSAARSSAPTKLFGVHLSAAPCG 368

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BLAST of MC11g0239 vs. ExPASy Swiss-Prot
Match: Q10KX8 (Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4D PE=3 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 7.5e-68
Identity = 145/257 (56.42%), Postives = 175/257 (68.09%), Query Frame = 0


Query: 48  MALMLDNCEG-VLLSLD-SH----------KAIPAPFLTKTYQLVDDPTTDHIVSWGEDD 107
           MA +++ C G +++S++ SH           A PAPFL+KTYQLVDDP+TD +VSWGED+
Sbjct: 1   MAFLVERCGGEMVVSMERSHGRSTTTAAAVTAAPAPFLSKTYQLVDDPSTDDVVSWGEDE 60

Query: 108 TTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLL 167
            TFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIV DRWEFANEFFRKG KHLL
Sbjct: 61  ATFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVADRWEFANEFFRKGAKHLL 120

Query: 168 CEIHRRKTAQ------PPQLTVNQHHHHS-----------PLGINAPSYYHFPGRLSISP 227
            EIHRRK++       PP   ++QH+  S           P+G  A + YHF        
Sbjct: 121 SEIHRRKSSSCSQPQPPPPFPMHQHYPLSLFSPPTTPRSPPVGAAAAAAYHF-------- 180

Query: 228 SESDDQPNWCDSPPLSSPTGGGNNNNNNNNNSVTALSEDNERLRRSNNMLMSELAHMKKL 276
                Q  +C SP  +   GGG        + + ALSEDN +LRR N++L+SELAHM+KL
Sbjct: 181 -----QEEYCSSP--ADYAGGG-------GDLLAALSEDNRQLRRRNSLLLSELAHMRKL 235

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BLAST of MC11g0239 vs. ExPASy Swiss-Prot
Match: Q6Z9R8 (Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4A PE=3 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.0e-56
Identity = 157/384 (40.89%), Postives = 201/384 (52.34%), Query Frame = 0


Query: 64  SHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDD-----TTFVVWRPPEFARDLLPNYFKHN 123
           S   +PAPFLTKTYQLVDDP TDH+VSW +DD     ++FVVWRPPEFARD+LPNYFKH+
Sbjct: 18  SASVVPAPFLTKTYQLVDDPATDHVVSWEDDDGGESASSFVVWRPPEFARDILPNYFKHS 77

Query: 124 NFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTA---------QPPQL 183
           NFSSFVRQLNTYGFRK+VP+RWEFANEFFRKGEK LLCEIHRRK+A          PP  
Sbjct: 78  NFSSFVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLCEIHRRKSAAATWPPFPPPPPPF 137

Query: 184 TVNQHHHHSPLGINAPSYYHFPGRLSISPSESDDQPNWCDSPPLSSPTGGGNNNNNNNNN 243
              +H        +     H  GRL    + + ++ +W +S  L  P    +   +    
Sbjct: 138 FAPRHFAAGAFFRHGDGMLH--GRLGALVT-TTERRHWFESAAL--PVAPSSRLLSQLGP 197

Query: 244 SVT------------ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSN 303
            +             AL ++N RL R N  L+ ELAHM+KLY+DIIYFVQNHV+PVAPS 
Sbjct: 198 VIAPARRAAATPEEEALMQENHRLLRGNAALVQELAHMRKLYSDIIYFVQNHVRPVAPSP 257

Query: 304 SYQYSTTSLLSDGFPARPPPSHLLGFHPTNPKPPVSGHAHFPTAPNNNNAPSRSSFVTIL 363
               +  + L      RPPP+   G  P +     SG            + + SS +T+ 
Sbjct: 258 ----AAAAALHGLGVLRPPPAG--GKAPASEVRGASG-----------RSATSSSSLTVA 317

Query: 364 DEPPAAPTCK--------------------TKLFGVPLQSKKRLHPEYSSNSNI----QI 398
           ++ P     +                    TKLFGV L S        +S        Q 
Sbjct: 318 EDQPTLLALRLPRTTEKIINEVSGGNGGGSTKLFGVHLSSADEQTSSGASRKRSPPQEQP 377

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BLAST of MC11g0239 vs. NCBI nr
Match: XP_022940239.1 (heat stress transcription factor B-4-like [Cucurbita moschata] >XP_023525267.1 heat stress transcription factor B-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 561 bits (1447), Expect = 7.69e-199
Identity = 295/356 (82.87%), Postives = 309/356 (86.80%), Query Frame = 0


Query: 48  MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 107
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 108 RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPP 167
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQP- 120

Query: 168 QLTVNQHHH-HSPLGINAPSYYHFPGRLSISPSESDDQPNWCDSPPLSSPTGGGNNNNNN 227
           Q+TVNQHH  +SPLG+NA  +YH+P R+SISPS+SDD PNWCDSPPLSS     NNNNNN
Sbjct: 121 QVTVNQHHQSYSPLGLNA-GFYHYPARVSISPSDSDDNPNWCDSPPLSS-----NNNNNN 180

Query: 228 NNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSL 287
            NNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST SL
Sbjct: 181 FNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST-SL 240

Query: 288 LSDGFPA--RPPPSHLLGFHPTNPKPPVSGHAHFPTAPNNNNAPSRSSFVTILDEPPAAP 347
           LSDGFP   R P  HLLG+HPTN K  VSG  HF     NNN  S  SFVTI++EP    
Sbjct: 241 LSDGFPVGQRQPNHHLLGYHPTNAKQ-VSGQTHFVAGTPNNNNGSSKSFVTIVEEP---- 300

Query: 348 TCKTKLFGVPLQSKKRLHPEYSSNSNIQIGGKDNNKARLVLEKDDLGLNLMPPSAC 400
             KTKLFGV LQSKKR+HPEY SN+ +    K+NNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 --KTKLFGVSLQSKKRVHPEYGSNNIV----KENNKARLVLEKDDLGLNLMPPSAC 337

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BLAST of MC11g0239 vs. NCBI nr
Match: KAG6608467.1 (Heat stress transcription factor B-4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 560 bits (1443), Expect = 3.62e-198
Identity = 294/356 (82.58%), Postives = 309/356 (86.80%), Query Frame = 0


Query: 48  MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 107
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 108 RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPP 167
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQP- 120

Query: 168 QLTVNQHHH-HSPLGINAPSYYHFPGRLSISPSESDDQPNWCDSPPLSSPTGGGNNNNNN 227
           Q+TVNQHH  +SPLG+NA  +YH+P R+SISPS+SDD PNWCDSPPLSS     +NNNNN
Sbjct: 121 QVTVNQHHQSYSPLGLNA-GFYHYPARVSISPSDSDDNPNWCDSPPLSS-NNNNSNNNNN 180

Query: 228 NNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSL 287
            NNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST SL
Sbjct: 181 FNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST-SL 240

Query: 288 LSDGFPA--RPPPSHLLGFHPTNPKPPVSGHAHFPTAPNNNNAPSRSSFVTILDEPPAAP 347
           LSDGFP   R P  HLLG+HPTN K  VSG  HF     NNN  S  SFVTI++EP    
Sbjct: 241 LSDGFPVGQRQPNHHLLGYHPTNAKQ-VSGQTHFVAGTPNNNNGSSKSFVTIVEEP---- 300

Query: 348 TCKTKLFGVPLQSKKRLHPEYSSNSNIQIGGKDNNKARLVLEKDDLGLNLMPPSAC 400
             KTKLFGV LQSKKR+HPEY SN+ +    K+NNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 --KTKLFGVSLQSKKRVHPEYGSNNIV----KENNKARLVLEKDDLGLNLMPPSAC 341

[Close]

BLAST of MC11g0239 vs. NCBI nr
Match: XP_022982267.1 (heat stress transcription factor B-4-like [Cucurbita maxima])

HSP 1 Score: 556 bits (1434), Expect = 8.17e-197
Identity = 299/360 (83.06%), Postives = 311/360 (86.39%), Query Frame = 0


Query: 48  MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 107
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 108 RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPP 167
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQP- 120

Query: 168 QLTVNQHHH-HSPLGINAPSYYHFPGRLSISPSESDDQPNWCDSPPLSSPTGGGNNNNNN 227
           Q+TVNQHH  +SPLG+NA  +YH+P R+SISPS+SDD PNWCDSPPLSS      NNNNN
Sbjct: 121 QVTVNQHHQSYSPLGLNA-GFYHYPARVSISPSDSDDNPNWCDSPPLSS------NNNNN 180

Query: 228 N--NNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTT 287
           N  NNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST 
Sbjct: 181 NVFNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST- 240

Query: 288 SLLSDGFPA--RPPPSHLLGFHPTNPKPPVSGHAHFP--TAPNNNNAPSRSSFVTILDEP 347
           SLLSDGFP   R P  HLLG+HPTN K  VSG  HF   T  NNNN  S  SFVTI++EP
Sbjct: 241 SLLSDGFPVGQRQPNHHLLGYHPTNAKQ-VSGQTHFVGGTPNNNNNNGSSKSFVTIVEEP 300

Query: 348 PAAPTCKTKLFGVPLQSKKRLHPEYSSNSNIQIGGKDNNKARLVLEKDDLGLNLMPPSAC 400
                 KTKLFGV LQSKKR+HPEY SN NI    K+NNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 ------KTKLFGVSLQSKKRVHPEYGSN-NI---AKENNKARLVLEKDDLGLNLMPPSAC 340

[Close]

BLAST of MC11g0239 vs. NCBI nr
Match: XP_038897232.1 (heat stress transcription factor B-4-like [Benincasa hispida])

HSP 1 Score: 535 bits (1377), Expect = 6.18e-188
Identity = 288/368 (78.26%), Postives = 302/368 (82.07%), Query Frame = 0


Query: 48  MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 107
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 108 RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPP 167
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQP- 120

Query: 168 QLTVNQHHH-HSPLGINAPSYYHFPGRLSISPSESDDQPNWCDSPPLSSPTGGGNNNNNN 227
           Q+TVNQHH  HSPLGIN P +YHFPGR+SISPS+SDDQ NWCDSP LSSP       +NN
Sbjct: 121 QVTVNQHHQSHSPLGINNPGFYHFPGRVSISPSDSDDQNNWCDSPSLSSPAP-----HNN 180

Query: 228 NNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSL 287
           NNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSL
Sbjct: 181 NNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSL 240

Query: 288 LSDGFPA---RPPPSHLLGFHPTNPKPPVSGHAHFPTAPNNN----------NAPSRSSF 347
           LSD FP    RPP  H   +H  +    V+G    PT  NNN          N     SF
Sbjct: 241 LSDRFPVVQQRPPNHHHHHYHQQSQIHLVTGTT--PTNNNNNHNNNNNIIINNNSGTKSF 300

Query: 348 VTILDEPPAAPTCKTKLFGVPLQSKKRLHPEYSSNSNIQIGGKDNN-KARLVLEKDDLGL 400
           VTIL+E P     KTKLFGV +QSKKRLHPEY SN+   IG ++NN KARLVLE DDLGL
Sbjct: 301 VTILEEQPT----KTKLFGVAIQSKKRLHPEYGSNN---IGKENNNNKARLVLENDDLGL 353

[Close]

BLAST of MC11g0239 vs. NCBI nr
Match: XP_023536624.1 (heat stress transcription factor B-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 513 bits (1321), Expect = 9.52e-180
Identity = 272/354 (76.84%), Postives = 293/354 (82.77%), Query Frame = 0


Query: 48  MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 107
           MALM+DNCEGVL+SLDSHK IPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMVDNCEGVLVSLDSHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 108 RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPP 167
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFAN+FFRKG+KHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQP- 120

Query: 168 QLTVNQHHHHSPLGINAPSYYHFPGRLSISPSESDDQPNWCDSPPLSSPTGGGNNNNNNN 227
           QL    H   SPL +N P +YHF GR SISPS+SDDQ NWCDSPPLSS    G NNNNNN
Sbjct: 121 QL----HQSLSPLALNNPGFYHFHGRGSISPSDSDDQNNWCDSPPLSS---SGTNNNNNN 180

Query: 228 NNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSLL 287
           NNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST  ++
Sbjct: 181 NNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTFPVV 240

Query: 288 SDGFPARPPPSHLLGFH-PTNPKPPVSGHAHFPTAPNNNNAPSRSSFVTILDEPPAAPTC 347
                 R P  HL+G++ PTN K  VSG  H  T   NNN  +  SFV I++E       
Sbjct: 241 Q-----RQPNHHLVGYNNPTNAKQ-VSGQTHLVTGIPNNNNKNNKSFVRIVEEQQ----- 300

Query: 348 KTKLFGVPLQSKKRLHPEYSSNSNIQIGGKDNNKARLVLEKDDLGLNLMPPSAC 400
           KTKLFGV + SKKRLHPEY+SN+   IG ++NNKAR VLEKDDLGLNLMPPSAC
Sbjct: 301 KTKLFGVAIHSKKRLHPEYASNN---IGKENNNKARFVLEKDDLGLNLMPPSAC 332

[Close]

BLAST of MC11g0239 vs. ExPASy TrEMBL
Match: A0A6J1FJH2 (heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC111445920 PE=3 SV=1)

HSP 1 Score: 561 bits (1447), Expect = 3.72e-199
Identity = 295/356 (82.87%), Postives = 309/356 (86.80%), Query Frame = 0


Query: 48  MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 107
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 108 RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPP 167
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQP- 120

Query: 168 QLTVNQHHH-HSPLGINAPSYYHFPGRLSISPSESDDQPNWCDSPPLSSPTGGGNNNNNN 227
           Q+TVNQHH  +SPLG+NA  +YH+P R+SISPS+SDD PNWCDSPPLSS     NNNNNN
Sbjct: 121 QVTVNQHHQSYSPLGLNA-GFYHYPARVSISPSDSDDNPNWCDSPPLSS-----NNNNNN 180

Query: 228 NNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSL 287
            NNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST SL
Sbjct: 181 FNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST-SL 240

Query: 288 LSDGFPA--RPPPSHLLGFHPTNPKPPVSGHAHFPTAPNNNNAPSRSSFVTILDEPPAAP 347
           LSDGFP   R P  HLLG+HPTN K  VSG  HF     NNN  S  SFVTI++EP    
Sbjct: 241 LSDGFPVGQRQPNHHLLGYHPTNAKQ-VSGQTHFVAGTPNNNNGSSKSFVTIVEEP---- 300

Query: 348 TCKTKLFGVPLQSKKRLHPEYSSNSNIQIGGKDNNKARLVLEKDDLGLNLMPPSAC 400
             KTKLFGV LQSKKR+HPEY SN+ +    K+NNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 --KTKLFGVSLQSKKRVHPEYGSNNIV----KENNKARLVLEKDDLGLNLMPPSAC 337

[Close]

BLAST of MC11g0239 vs. ExPASy TrEMBL
Match: A0A6J1IW73 (heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC111481146 PE=3 SV=1)

HSP 1 Score: 556 bits (1434), Expect = 3.95e-197
Identity = 299/360 (83.06%), Postives = 311/360 (86.39%), Query Frame = 0


Query: 48  MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 107
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 108 RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPP 167
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQP- 120

Query: 168 QLTVNQHHH-HSPLGINAPSYYHFPGRLSISPSESDDQPNWCDSPPLSSPTGGGNNNNNN 227
           Q+TVNQHH  +SPLG+NA  +YH+P R+SISPS+SDD PNWCDSPPLSS      NNNNN
Sbjct: 121 QVTVNQHHQSYSPLGLNA-GFYHYPARVSISPSDSDDNPNWCDSPPLSS------NNNNN 180

Query: 228 N--NNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTT 287
           N  NNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST 
Sbjct: 181 NVFNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST- 240

Query: 288 SLLSDGFPA--RPPPSHLLGFHPTNPKPPVSGHAHFP--TAPNNNNAPSRSSFVTILDEP 347
           SLLSDGFP   R P  HLLG+HPTN K  VSG  HF   T  NNNN  S  SFVTI++EP
Sbjct: 241 SLLSDGFPVGQRQPNHHLLGYHPTNAKQ-VSGQTHFVGGTPNNNNNNGSSKSFVTIVEEP 300

Query: 348 PAAPTCKTKLFGVPLQSKKRLHPEYSSNSNIQIGGKDNNKARLVLEKDDLGLNLMPPSAC 400
                 KTKLFGV LQSKKR+HPEY SN NI    K+NNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 ------KTKLFGVSLQSKKRVHPEYGSN-NI---AKENNKARLVLEKDDLGLNLMPPSAC 340

[Close]

BLAST of MC11g0239 vs. ExPASy TrEMBL
Match: A0A6J1IFM1 (heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC111476507 PE=3 SV=1)

HSP 1 Score: 508 bits (1308), Expect = 4.70e-178
Identity = 269/355 (75.77%), Postives = 292/355 (82.25%), Query Frame = 0


Query: 48  MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 107
           MALM+DNCEGVL+SL+ HK IPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMVDNCEGVLVSLEPHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 108 RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPP 167
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFAN+FFRKG+KHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQP- 120

Query: 168 QLTVNQHHHH-SPLGINAPSYYHFPGRLSISPSESDDQPNWCDSPPLSSPTGGGNNNNNN 227
                QHH   SPL +N P +YHF GR SISPS+SDDQ NWCDSPPLSS +G  NNNNNN
Sbjct: 121 -----QHHQSLSPLALNNPGFYHFHGRGSISPSDSDDQNNWCDSPPLSS-SGTNNNNNNN 180

Query: 228 NNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSL 287
            +NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST  +
Sbjct: 181 YSNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTFPV 240

Query: 288 LSDGFPARPPPSHLLGFH-PTNPKPPVSGHAHFPTAPNNNNAPSRSSFVTILDEPPAAPT 347
           +      R P  HL+G++ PTN K  VSG  H  T   NNN  S  S V I++E      
Sbjct: 241 VQ-----RQPNHHLVGYNNPTNAKR-VSGQTHLVTGTPNNNNDSNKSLVRIVEEQQ---- 300

Query: 348 CKTKLFGVPLQSKKRLHPEYSSNSNIQIGGKDNNKARLVLEKDDLGLNLMPPSAC 400
            KTKLFGV + SKKRLHPEY+SN+   IG ++NNKAR VLEKDDLGLNLMPPSAC
Sbjct: 301 -KTKLFGVAIHSKKRLHPEYASNN---IGKENNNKARFVLEKDDLGLNLMPPSAC 334

[Close]

BLAST of MC11g0239 vs. ExPASy TrEMBL
Match: A0A0A0L4W8 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639930 PE=3 SV=1)

HSP 1 Score: 503 bits (1295), Expect = 3.72e-176
Identity = 279/359 (77.72%), Postives = 290/359 (80.78%), Query Frame = 0


Query: 48  MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 107
           MALMLD C+GVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 108 RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPP 167
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQP- 120

Query: 168 QLTVNQHHH-HSPLGINAPSYYHFP-GRLSISPSESDDQPN-WCDSPPLSSPTGGGNNNN 227
           Q+TVNQHH  HSPL    P +YHFP  RLSISPS+SDDQ N WCDSP   SP       N
Sbjct: 121 QVTVNQHHQPHSPLN---PGFYHFPTARLSISPSDSDDQNNYWCDSP---SP-------N 180

Query: 228 NNNNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTT 287
           NNNNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTT
Sbjct: 181 NNNNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTT 240

Query: 288 -SLLSDGFPARPPPSHLLGFHPTNPKPPVSGHAHFPTAPN-NNNAPSRSSFVTILDEPPA 347
            SLLSDGFP    P+H    H          H H   +    NN     SFVTIL+E   
Sbjct: 241 TSLLSDGFPVVRQPNHYHHHH----------HHHQQVSSQIRNNTVGTKSFVTILEEEQQ 300

Query: 348 APTCKTKLFGVPLQSKKRLHPEY-SSNSNIQIGGKDNNKARLVLEKDDLGLNLMPPSAC 400
             T KTKLFGV +QSKKRLHPEY +SNSN      +NNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 QQT-KTKLFGVAIQSKKRLHPEYGNSNSN-----NNNNKARLVLEKDDLGLNLMPPSAC 329

[Close]

BLAST of MC11g0239 vs. ExPASy TrEMBL
Match: A0A6J1F8Q8 (heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC111443150 PE=3 SV=1)

HSP 1 Score: 500 bits (1288), Expect = 4.32e-175
Identity = 265/355 (74.65%), Postives = 289/355 (81.41%), Query Frame = 0


Query: 48  MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 107
           MALM+DNCEGVL+SL+SHK IPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMVDNCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 108 RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPP 167
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFAN+FFRKG+KHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQP- 120

Query: 168 QLTVNQHHHH-SPLGINAPSYYHFPGRLSISPSESDDQPNWCDSPPLSSPTGGGNNNNNN 227
                QH    SPL +N P +YHF  R SISPS+SDDQ NWCDSPPLSS      +  NN
Sbjct: 121 -----QHQQSLSPLALNNPGFYHFHARPSISPSDSDDQNNWCDSPPLSS------SGTNN 180

Query: 228 NNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSL 287
           NNNSV+ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST  +
Sbjct: 181 NNNSVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTFPV 240

Query: 288 LSDGFPARPPPSHLLGFH-PTNPKPPVSGHAHFPTAPNNNNAPSRSSFVTILDEPPAAPT 347
           +      R P  HL+G++ PTN K  VSG  H  TA  NNN  +  SFV I++E      
Sbjct: 241 VQ-----RQPNHHLVGYNNPTNAKQ-VSGQTHLVTATPNNNNNNNKSFVRIVEEQQ---- 300

Query: 348 CKTKLFGVPLQSKKRLHPEYSSNSNIQIGGKDNNKARLVLEKDDLGLNLMPPSAC 400
            KTKLFGV + SKKRLHPEY+SN+   IG ++NNKAR VLEKDDLGLNLMPPSAC
Sbjct: 301 -KTKLFGVAIHSKKRLHPEYASNN---IGKENNNKARFVLEKDDLGLNLMPPSAC 329

[Close]

BLAST of MC11g0239 vs. TAIR 10
Match: AT1G46264.1 (heat shock transcription factor B4 )

HSP 1 Score: 323.2 bits (827), Expect = 3.0e-88
Identity = 196/370 (52.97%), Postives = 236/370 (63.78%), Query Frame = 0


Query: 48  MALMLDNCEG----------VLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTT 107
           MA+M++N  G           L+     KA+PAPFLTKTYQLVDDP TDH+VSWG+DDTT
Sbjct: 1   MAMMVENSYGGYGGGGGERIQLMVEGQGKAVPAPFLTKTYQLVDDPATDHVVSWGDDDTT 60

Query: 108 FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCE 167
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFF++GEKHLLCE
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFKRGEKHLLCE 120

Query: 168 IHRRKTAQ--PPQLTVNQHHHHSP--LGINAPSYYHFPGRLSISPSESDDQPNWCDSPPL 227
           IHRRKT+Q  P Q +    HHH+P  +  +  S++  P     +P E      WCD  P 
Sbjct: 121 IHRRKTSQMIPQQHSPFMSHHHAPPQIPFSGGSFFPLPPPRVTTPEEDH---YWCDDSPP 180

Query: 228 SSPTGGGNNNNNNNNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPV 287
           S P         +    VTALSEDNERLRRSN +LMSELAHMKKLYNDIIYFVQNHVKPV
Sbjct: 181 SRPR--VIPQQIDTAAQVTALSEDNERLRRSNTVLMSELAHMKKLYNDIIYFVQNHVKPV 240

Query: 288 APSNSYQYSTTSLLSDGFPARPPPSHLLGFHPTNPKPPVSGHAHFPTAPNNNNAPSRSSF 347
           APSN+  Y  +S L      +PP    L ++ T      + +A       N++ P+  S 
Sbjct: 241 APSNNSSY-LSSFLQKQQQQQPP---TLDYYNTATVNATNLNA------LNSSPPTSQSS 300

Query: 348 VTILDEP-----PAAPTCKTKLFGVPLQSKKRLHPEYSSNSNIQIGGKDNNKARLVLEKD 399
           +T+L++        +   KTKLFGV L S K+    +S  +         +K RLVL++ 
Sbjct: 301 ITVLEDDHTNHHDQSNMRKTKLFGVSLPSSKKRSHHFSDQT---------SKTRLVLDQS 346

[Close]

BLAST of MC11g0239 vs. TAIR 10
Match: AT4G11660.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 183.0 bits (463), Expect = 4.9e-46
Identity = 105/214 (49.07%), Postives = 136/214 (63.55%), Query Frame = 0


Query: 63  DSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSS 122
           DS ++IP PFLTKTYQLV+DP  D ++SW ED TTF+VWRP EFARDLLP YFKHNNFSS
Sbjct: 51  DSQRSIPTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFKHNNFSS 110

Query: 123 FVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPPQLTVNQHHHHSPLGI 182
           FVRQLNTYGFRK+VPDRWEF+N+ F++GEK LL +I RRK +Q P +        + +  
Sbjct: 111 FVRQLNTYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRRKISQ-PAMAAAAAAAAAAVAA 170

Query: 183 NAPSYYHFPGRLSI-SPSES-DDQPNWCDSPP------LSSPTGGGNNNNNNNNNSVTAL 242
           +A +    P    I SPS S ++Q    +S P      +    GGG+     +  +   L
Sbjct: 171 SAVTVAAVPVVAHIVSPSNSGEEQVISSNSSPAAAAAAIGGVVGGGSLQRTTSCTTAPEL 230

Query: 243 SEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQN 269
            E+NERLR+ N  L  E+  +K LY +I   + N
Sbjct: 231 VEENERLRKDNERLRKEMTKLKGLYANIYTLMAN 263

[Close]

BLAST of MC11g0239 vs. TAIR 10
Match: AT4G36990.1 (heat shock factor 4 )

HSP 1 Score: 176.4 bits (446), Expect = 4.6e-44
Identity = 98/209 (46.89%), Postives = 132/209 (63.16%), Query Frame = 0


Query: 64  SHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSF 123
           + +++PAPFL+KTYQLVDD +TD +VSW E+ T FVVW+  EFA+DLLP YFKHNNFSSF
Sbjct: 7   AQRSVPAPFLSKTYQLVDDHSTDDVVSWNEEGTAFVVWKTAEFAKDLLPQYFKHNNFSSF 66

Query: 124 VRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPPQLTVNQHHHHSPLGIN 183
           +RQLNTYGFRK VPD+WEFAN++FR+G + LL +I RRK               S +   
Sbjct: 67  IRQLNTYGFRKTVPDKWEFANDYFRRGGEDLLTDIRRRK---------------SVIAST 126

Query: 184 APSYYHFPGRLSISPSESDDQPNWCDSPPLSSPTGGGNNNNNNN-NNSVTALSEDNERLR 243
           A       G+  +  S S+      D    SS +  G++ N  +  N V  LS +NE+L+
Sbjct: 127 A-------GKCVVVGSPSESNSGGGDDHGSSSTSSPGSSKNPGSVENMVADLSGENEKLK 186

Query: 244 RSNNMLMSELAHMKKLYNDIIYFVQNHVK 272
           R NN L SELA  KK  ++++ F+  H+K
Sbjct: 187 RENNNLSSELAAAKKQRDELVTFLTGHLK 193

[Close]

BLAST of MC11g0239 vs. TAIR 10
Match: AT5G62020.1 (heat shock transcription factor B2A )

HSP 1 Score: 170.2 bits (430), Expect = 3.3e-42
Identity = 97/218 (44.50%), Postives = 128/218 (58.72%), Query Frame = 0


Query: 64  SHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSF 123
           S ++IP PFLTKT+ LV+D + D ++SW ED ++F+VW P +FA+DLLP +FKHNNFSSF
Sbjct: 16  SQRSIPTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHNNFSSF 75

Query: 124 VRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPPQLTVNQHHHHSPLGIN 183
           VRQLNTYGF+K+VPDRWEF+N+FF++GEK LL EI RRK     Q  V            
Sbjct: 76  VRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRKITTTHQTVV------------ 135

Query: 184 APSYYHFPGRLSISPSESDDQPNWCDSPPLSSPTGG--GNNNNNNNNNSVTALSEDNERL 243
           APS       + +SPS S +  N  +    SSP+           N      L E+NE+L
Sbjct: 136 APSSEQRNQTMVVSPSNSGEDNN-NNQVMSSSPSSWYCHQTKTTGNGGLSVELLEENEKL 195

Query: 244 RRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSY 280
           R  N  L  EL  MK + ++I   + N+V       SY
Sbjct: 196 RSQNIQLNRELTQMKSICDNIYSLMSNYVGSQPTDRSY 220

[Close]

BLAST of MC11g0239 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 159.1 bits (401), Expect = 7.5e-39
Identity = 69/99 (69.70%), Postives = 83/99 (83.84%), Query Frame = 0


Query: 67  AIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQ 126
           ++P PFL+KTY +V+DP TD IVSW   + +F+VW PPEF+RDLLP YFKHNNFSSFVRQ
Sbjct: 48  SLPPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQ 107

Query: 127 LNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQ 166
           LNTYGFRK+ PDRWEFANE F +G+KHLL +I RRK+ Q
Sbjct: 108 LNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQ 146

The following BLAST results are available for this feature:

BLAST of MC11g0239 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C635	4.2e-87	52.97	Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE...	[more]
Q7XHZ0	5.8e-68	47.15	Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q67U94	5.8e-68	44.44	Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q10KX8	7.5e-68	56.42	Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q6Z9R8	1.0e-56	40.89	Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica O...	[more]

BLAST of MC11g0239 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022940239.1	7.69e-199	82.87	heat stress transcription factor B-4-like [Cucurbita moschata] >XP_023525267.1 h...	[more]
KAG6608467.1	3.62e-198	82.58	Heat stress transcription factor B-4, partial [Cucurbita argyrosperma subsp. sor...	[more]
XP_022982267.1	8.17e-197	83.06	heat stress transcription factor B-4-like [Cucurbita maxima]	[more]
XP_038897232.1	6.18e-188	78.26	heat stress transcription factor B-4-like [Benincasa hispida]	[more]
XP_023536624.1	9.52e-180	76.84	heat stress transcription factor B-4-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC11g0239 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FJH2	3.72e-199	82.87	heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1IW73	3.95e-197	83.06	heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1IFM1	4.70e-178	75.77	heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A0A0L4W8	3.72e-176	77.72	HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639930 ...	[more]
A0A6J1F8Q8	4.32e-175	74.65	heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

BLAST of MC11g0239 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G46264.1	3.0e-88	52.97	heat shock transcription factor B4	[more]
AT4G11660.1	4.9e-46	49.07	winged-helix DNA-binding transcription factor family protein	[more]
AT4G36990.1	4.6e-44	46.89	heat shock factor 4	[more]
AT5G62020.1	3.3e-42	44.50	heat shock transcription factor B2A	[more]
AT4G17750.1	7.5e-39	69.70	heat shock factor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 231..251
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 197..235
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 193..238
None	No IPR available	PANTHER	PTHR10015:SF382	BNAC06G00310D PROTEIN	coord: 59..398
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PRINTS	PR00056	HSFDOMAIN	coord: 110..122 score: 78.68 coord: 123..135 score: 84.62 coord: 72..95 score: 46.19
IPR000232	Heat shock factor (HSF)-type, DNA-binding	SMART	SM00415	hsfneu3	coord: 68..161 e-value: 2.3E-59 score: 213.2
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PFAM	PF00447	HSF_DNA-bind	coord: 72..161 e-value: 4.4E-32 score: 110.6
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PROSITE	PS00434	HSF_DOMAIN	coord: 111..135
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 64..163 e-value: 3.3E-37 score: 129.1
IPR027725	Heat shock transcription factor family	PANTHER	PTHR10015	HEAT SHOCK TRANSCRIPTION FACTOR	coord: 59..398
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 69..161

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g0239.1	MC11g0239.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0000978	RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function	GO:0043565	sequence-specific DNA binding