MC11g0171 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC11g0171
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description(1->3)-beta-glucan endohydrolase
LocationMC11: 1262771 .. 1265592 (+)
RNA-Seq ExpressionMC11g0171
SyntenyMC11g0171
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGGAAGTTGGGCAACGGTCAAGAAAAAAGGGCATTTGGGTCATTCAGAAAAACATAGACAATATAGCTTTTTATATGTTTTTTAAATTATTATTCTAAGCTCGACGTTACTCGCGCAACTCATGGGAGACTTCCACGACTCTTCCAGCTCTCACTTCCACTTCTCTAACGGTGGAATTCATTCACCGCTCTTGTCGGGCAGCCCGCTTTTGAAAAAGTAAAGAGGGCCCACCGAATTTTAATTTTACTATACTACCCTTGTCCTCTTCCTTGCTTCTCCATCTATTTAATTTCTTCTTCCTCTGCGTCACGTCTCCAACATATCGCAACTTCCAAACTCATCGGCCATGGCCTCCACTCTTCTTCGTGCTACTGTTTTCTTCTTCCTCAACATTCTATTGGTCGCTGATTTCGCCTGTAAGTTTCTGGTTTCTCTTTTTGTTATATGTGAATCTTTTACCAACTACTGGAGATTTTTCTACCTTTTCAAGTTTTCATGTTCGTATAGTCTTCCAGTTTCGTTTTCTGGTTATGTTTATTGAGAAAATAAATTGGACTCTCTGTGAATTTCCCGTAGCGATAGTCTTTTGAAGCTTGATTGTGAATCTTGTGATGGAGATTCTATAACAGGGGAAGATTGAAAGAAAACGAAAGAGAGAGAAAAAGGAGATGGAGAATTTTCAGTAAAGAATCGAAAGAAAGGGTGAAAAGTTTATCAGTCTAATTTGAATAGCCTCTGTTTTGCAGACTCGCAATCGTTCATCGGAATCAACTACGGTCAAGTCGCCGATAACCTTCCTCCGCCGTCGGCGACGGCGAAGTTACTTCAATCAACTTCAATCGAGAAAGTTCGACTCTATGGTGCTGATCCGGCCATCATCAAAGCCCTAGCAAACACGGGGATCGGAATTGTAATCGGCGCTGCCAACGGTGACATTCCAGCCCTAGCGTCCGACCCGAATTTCGCCCAAAATTGGGTTAACGCGAATGTAGCCCCCTTCTACCCGGCGAGCAAAATCATCCTCATCACGGTCGGCAACGAGGTGATAACTTCCAATGATCAAAATCTGATGAACCAGCTCTTGCCGGCAATTCAGAATCTCCAAAACGCGCTGAATTCGATATCTCTGGGGAGTAATATTAAGGTTTCCACTGTCCACTCAATGGCGGTGCTTCGCCAATCGGAGCCGCCATCGTCTGCGGTCTTCCACCCCAACTATATGAGCGTGTTGAAGGGGCTACTGGAGTTCAACAGCGCGACTGGATCGCCATTCACCATTAACCCGTACCCATACTTCGCGTATCAAAGCGACCCGCGGCCGGAGACTTTGGCGTTCTGTCTTTTCCAGCCCAACGCCGGGCGGCTCGATACAAACACCAACATCAAGTACATGAACATGTTCGACGCTCAGGTGAGTTCACGAATCTTCAAGCCCTGTTCTGGTCTGTGTGTTCTTAGGATTTTCTTTTTTTTCTTAAATGTTATTTGCCACCGGTTGGATTCAGGTGGATGCCATAAGATCGGCGTTGAACTCCATGGGCTTCAAGAACGTGGAGATTGTGGTGGCGGAGACTGGTTGGCCGTACAAAGGAGACGGCGGCGAGGTTGGCCCAGGTCTCGAGAATGCTAAAGCATTCAACGGTAATCTGATTGCGCACCTCCGATCCATGGTTGGGACGCCATTGATGCCAGGAAAATCAGTGGATACGTATCTCTTTGCGCTGTACGACGAAGATTTGAAACCTGGGCCTGGCTCTGAGAGAGCTTTCGGATTGTTCAAACCTGATCTTACAATGACGTACGACGTCGGCCTTTCAAAAAGTGGCCAGGTAACAGAGTAATTCACAAAACCCCCGAATAATTTCTGTTAAATTAAATCGTAATCTTCTATTTTTGAAGAATATTACGAATGAATTGCTTATCGTCTTCAACAGAGTACTCCGGCTTCTCCCAAAACACCAGCGACGACTCCATCGTCCCCGGCAAATCCATCGACGAAAGCACCGGCGGGTCCAAAGCCAAAAGCAGGGGGAGGGTCATGGTGCGTGCCGATGGGCGGTGTATCAGACGCCCAGCTCCAAGCCAATTTGGACTACGCGTGCGGAAGAGGGCTTGATTGCAGCGCCATTCAGCCCGGCGGCGCGTGTTTCGAGCCAAACACCATAGCCTCACACGCCGCCTACGCCATGAACCTCTTCTTCCAGGCCGCCGGCCGAGATCCATGGACCTGTGACTTCTCACACACTGCCACACTTTCCTCCAACAATCCAAGTAAGTACTAAGGATCCATTAACAAAATGTTAGTTGTTTCTTGTTTACATTTCAAACGTTTAGATTTTCTCTATAAAATTAGAAGCTGAAGGTTTGATTGATGAAATTGAGAATTCAAACTTCTATTAAAATAGGGCTGAATTATTAGAAGGCTAAACTTTGTTTTGTGCTTTTTGGTGCAGGCTACAATGGGTGCAATTATCCTGGTGGGAGCACGTGAATGTGATGTAAATTTGAGGAAGAAGAGAGAGGCTTGCAAGTTCTCAGCAGGTCGACTGGAGGATAAAGATCAAGATTCCAACTCCATTAATATGTATATTTTTCTGATGGTTTTGAAAATAATTTTTTTTTTCTAAATATTATATAATATCGGTTTTAGCTTTGACTACTGAGAGCATGTTTGTTGTAAATTGTAAATTCTGATTGTGTGTAATGTGCCCCTGAAGAAATGTTAAATTGGTGGTGTTTTGCTTTCCTCTCAAATGTATTCTGTTCCTTAGTTATTTTGATTGTCGGTATTTAATTATAATATCATCCATTTTTCCCCGTA

mRNA sequence

AAGGAAGTTGGGCAACGGTCAAGAAAAAAGGGCATTTGGGTCATTCAGAAAAACATAGACAATATAGCTTTTTATATGTTTTTTAAATTATTATTCTAAGCTCGACGTTACTCGCGCAACTCATGGGAGACTTCCACGACTCTTCCAGCTCTCACTTCCACTTCTCTAACGGTGGAATTCATTCACCGCTCTTGTCGGGCAGCCCGCTTTTGAAAAAGTAAAGAGGGCCCACCGAATTTTAATTTTACTATACTACCCTTGTCCTCTTCCTTGCTTCTCCATCTATTTAATTTCTTCTTCCTCTGCGTCACGTCTCCAACATATCGCAACTTCCAAACTCATCGGCCATGGCCTCCACTCTTCTTCGTGCTACTGTTTTCTTCTTCCTCAACATTCTATTGGTCGCTGATTTCGCCTACTCGCAATCGTTCATCGGAATCAACTACGGTCAAGTCGCCGATAACCTTCCTCCGCCGTCGGCGACGGCGAAGTTACTTCAATCAACTTCAATCGAGAAAGTTCGACTCTATGGTGCTGATCCGGCCATCATCAAAGCCCTAGCAAACACGGGGATCGGAATTGTAATCGGCGCTGCCAACGGTGACATTCCAGCCCTAGCGTCCGACCCGAATTTCGCCCAAAATTGGGTTAACGCGAATGTAGCCCCCTTCTACCCGGCGAGCAAAATCATCCTCATCACGGTCGGCAACGAGGTGATAACTTCCAATGATCAAAATCTGATGAACCAGCTCTTGCCGGCAATTCAGAATCTCCAAAACGCGCTGAATTCGATATCTCTGGGGAGTAATATTAAGGTTTCCACTGTCCACTCAATGGCGGTGCTTCGCCAATCGGAGCCGCCATCGTCTGCGGTCTTCCACCCCAACTATATGAGCGTGTTGAAGGGGCTACTGGAGTTCAACAGCGCGACTGGATCGCCATTCACCATTAACCCGTACCCATACTTCGCGTATCAAAGCGACCCGCGGCCGGAGACTTTGGCGTTCTGTCTTTTCCAGCCCAACGCCGGGCGGCTCGATACAAACACCAACATCAAGTACATGAACATGTTCGACGCTCAGGTGGATGCCATAAGATCGGCGTTGAACTCCATGGGCTTCAAGAACGTGGAGATTGTGGTGGCGGAGACTGGTTGGCCGTACAAAGGAGACGGCGGCGAGGTTGGCCCAGGTCTCGAGAATGCTAAAGCATTCAACGGTAATCTGATTGCGCACCTCCGATCCATGGTTGGGACGCCATTGATGCCAGGAAAATCAGTGGATACGTATCTCTTTGCGCTGTACGACGAAGATTTGAAACCTGGGCCTGGCTCTGAGAGAGCTTTCGGATTGTTCAAACCTGATCTTACAATGACGTACGACGTCGGCCTTTCAAAAAGTGGCCAGAGTACTCCGGCTTCTCCCAAAACACCAGCGACGACTCCATCGTCCCCGGCAAATCCATCGACGAAAGCACCGGCGGGTCCAAAGCCAAAAGCAGGGGGAGGGTCATGGTGCGTGCCGATGGGCGGTGTATCAGACGCCCAGCTCCAAGCCAATTTGGACTACGCGTGCGGAAGAGGGCTTGATTGCAGCGCCATTCAGCCCGGCGGCGCGTGTTTCGAGCCAAACACCATAGCCTCACACGCCGCCTACGCCATGAACCTCTTCTTCCAGGCCGCCGGCCGAGATCCATGGACCTGTGACTTCTCACACACTGCCACACTTTCCTCCAACAATCCAAGCTACAATGGGTGCAATTATCCTGGTGGGAGCACGTGAATGTGATGTAAATTTGAGGAAGAAGAGAGAGGCTTGCAAGTTCTCAGCAGGTCGACTGGAGGATAAAGATCAAGATTCCAACTCCATTAATATGTATATTTTTCTGATGGTTTTGAAAATAATTTTTTTTTTCTAAATATTATATAATATCGGTTTTAGCTTTGACTACTGAGAGCATGTTTGTTGTAAATTGTAAATTCTGATTGTGTGTAATGTGCCCCTGAAGAAATGTTAAATTGGTGGTGTTTTGCTTTCCTCTCAAATGTATTCTGTTCCTTAGTTATTTTGATTGTCGGTATTTAATTATAATATCATCCATTTTTCCCCGTA

Coding sequence (CDS)

ATGGCCTCCACTCTTCTTCGTGCTACTGTTTTCTTCTTCCTCAACATTCTATTGGTCGCTGATTTCGCCTACTCGCAATCGTTCATCGGAATCAACTACGGTCAAGTCGCCGATAACCTTCCTCCGCCGTCGGCGACGGCGAAGTTACTTCAATCAACTTCAATCGAGAAAGTTCGACTCTATGGTGCTGATCCGGCCATCATCAAAGCCCTAGCAAACACGGGGATCGGAATTGTAATCGGCGCTGCCAACGGTGACATTCCAGCCCTAGCGTCCGACCCGAATTTCGCCCAAAATTGGGTTAACGCGAATGTAGCCCCCTTCTACCCGGCGAGCAAAATCATCCTCATCACGGTCGGCAACGAGGTGATAACTTCCAATGATCAAAATCTGATGAACCAGCTCTTGCCGGCAATTCAGAATCTCCAAAACGCGCTGAATTCGATATCTCTGGGGAGTAATATTAAGGTTTCCACTGTCCACTCAATGGCGGTGCTTCGCCAATCGGAGCCGCCATCGTCTGCGGTCTTCCACCCCAACTATATGAGCGTGTTGAAGGGGCTACTGGAGTTCAACAGCGCGACTGGATCGCCATTCACCATTAACCCGTACCCATACTTCGCGTATCAAAGCGACCCGCGGCCGGAGACTTTGGCGTTCTGTCTTTTCCAGCCCAACGCCGGGCGGCTCGATACAAACACCAACATCAAGTACATGAACATGTTCGACGCTCAGGTGGATGCCATAAGATCGGCGTTGAACTCCATGGGCTTCAAGAACGTGGAGATTGTGGTGGCGGAGACTGGTTGGCCGTACAAAGGAGACGGCGGCGAGGTTGGCCCAGGTCTCGAGAATGCTAAAGCATTCAACGGTAATCTGATTGCGCACCTCCGATCCATGGTTGGGACGCCATTGATGCCAGGAAAATCAGTGGATACGTATCTCTTTGCGCTGTACGACGAAGATTTGAAACCTGGGCCTGGCTCTGAGAGAGCTTTCGGATTGTTCAAACCTGATCTTACAATGACGTACGACGTCGGCCTTTCAAAAAGTGGCCAGAGTACTCCGGCTTCTCCCAAAACACCAGCGACGACTCCATCGTCCCCGGCAAATCCATCGACGAAAGCACCGGCGGGTCCAAAGCCAAAAGCAGGGGGAGGGTCATGGTGCGTGCCGATGGGCGGTGTATCAGACGCCCAGCTCCAAGCCAATTTGGACTACGCGTGCGGAAGAGGGCTTGATTGCAGCGCCATTCAGCCCGGCGGCGCGTGTTTCGAGCCAAACACCATAGCCTCACACGCCGCCTACGCCATGAACCTCTTCTTCCAGGCCGCCGGCCGAGATCCATGGACCTGTGACTTCTCACACACTGCCACACTTTCCTCCAACAATCCAAGCTACAATGGGTGCAATTATCCTGGTGGGAGCACGTGA

Protein sequence

MASTLLRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRLYGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVGNEVITSNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPNYMSVLKGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSMVGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPKTPATTPSSPANPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQPGGACFEPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGGST
Homology
BLAST of MC11g0171 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 635.2 bits (1637), Expect = 6.0e-181
Identity = 312/470 (66.38%), Postives = 367/470 (78.09%), Query Frame = 0

Query: 6   LRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRLYGADP 65
           L  +++F L  L     ++++ FIG+NYGQVADNLPPPS T KLLQSTSI+KVRLYGADP
Sbjct: 3   LSISIYFLLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADP 62

Query: 66  AIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVGNEVIT 125
           AIIKALA TG+GIVIGAANGD+P+LASDPN A  W+N+NV PFYPASKI+LITVGNE++ 
Sbjct: 63  AIIKALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILM 122

Query: 126 SNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPNYMSVL 185
           SND NL+NQLLPA+QN+Q AL ++SLG  IKVSTV+SM VL  S+PPSS  F   Y + L
Sbjct: 123 SNDPNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGL 182

Query: 186 KGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMFDAQ 245
           KG+L+F S TGSPF INPYP+FAYQSDPRPETLAFCLF+PNAGR+D+ T IKY NMFDAQ
Sbjct: 183 KGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQ 242

Query: 246 VDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSMVGTPL 305
           VDA+ SAL SMGF+ VEIVVAETGW  +GD  EVG  ++NAKA+NGNLIAHLRSMVGTPL
Sbjct: 243 VDAVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPL 302

Query: 306 MPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPKTPATT 365
           MPGK VDTY+FALYDE+LKPGP SERAFGLFK DL+M YDVGL+KS  S+          
Sbjct: 303 MPGKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAKSSSSSQT-------- 362

Query: 366 PSSPANPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQPGGACF 425
                         P  K     WCVP  G ++ +LQA+LD+ACG G+DC AIQPGGACF
Sbjct: 363 --------------PSGKVTSSGWCVPKKGATNEELQASLDWACGHGIDCGAIQPGGACF 422

Query: 426 EPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGG 476
           EPN + SHAAYAMN++FQ + + P  CDFS TAT++S NPSYN C YPGG
Sbjct: 423 EPNNVVSHAAYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYPGG 450

BLAST of MC11g0171 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 3.7e-170
Identity = 298/473 (63.00%), Postives = 363/473 (76.74%), Query Frame = 0

Query: 6   LRATVFFFLNILLVADF--AYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRLYGA 65
           ++ +   FL  LL+ +F  A SQSF+G+NYGQ++DNLP   AT  LL+ST+I+KVRL+GA
Sbjct: 5   VQTSSLLFLVFLLLQNFYSANSQSFLGVNYGQLSDNLPSLQATVNLLKSTTIQKVRLFGA 64

Query: 66  DPAIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVGNEV 125
           +PA+IKA ANTG+ IVIG  NGDIP LAS+PN A  +V +NV  FYPAS II ITVGNEV
Sbjct: 65  EPAVIKAFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEV 124

Query: 126 ITSNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPNYMS 185
           +TS DQ L++QLLPA+QN+QNALN+ SLG  +KVSTVH+MAVL QS PPSS VF+P    
Sbjct: 125 LTSGDQKLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLGD 184

Query: 186 VLKGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMFD 245
            +K LL+F SA  +PF I+PYPYFAY++ P P+TLAFCLFQPNAG++D+    KY NMFD
Sbjct: 185 TMKALLQFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFD 244

Query: 246 AQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSMVGT 305
           AQVDA+ SALN+MGFK++EIVVAETGWP+ GD  EVGP L+NAKA+ GNLI HL+S VGT
Sbjct: 245 AQVDAVHSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGT 304

Query: 306 PLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPKTPA 365
           PLMPGKS+DTYLF+LYDED K G  SE+ FGLFKPD + TYDVGL K+ Q          
Sbjct: 305 PLMPGKSIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDVGLLKNTQ---------- 364

Query: 366 TTPSSPANPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQPGGA 425
                  NP+T A   P PKA  GSWCVP  GVSD QL  N++YACG+G+DC  IQPGGA
Sbjct: 365 -------NPTTPATPTPTPKA-AGSWCVPKPGVSDDQLTGNINYACGQGIDCGPIQPGGA 424

Query: 426 CFEPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGGS 477
           CFEPNT+ +HAAY MNL++Q+AGR+ W CDFS TATL++ NPSY  CN+P GS
Sbjct: 425 CFEPNTVKAHAAYVMNLYYQSAGRNSWNCDFSQTATLTNTNPSYGACNFPSGS 459

BLAST of MC11g0171 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.0e-103
Identity = 200/459 (43.57%), Postives = 290/459 (63.18%), Query Frame = 0

Query: 20  ADFAYSQSFIGINYGQVADNLPPPSATAKLLQS-TSIEKVRLYGADPAIIKALANTGIGI 79
           A+ A     IG+NYG  ADNLP P++ A  L + T+I++V+L+ A+P  I A A T I +
Sbjct: 17  AESATPSLHIGVNYGANADNLPSPTSVATFLATKTTIDRVKLFDANPTFISAFAGTPISL 76

Query: 80  VIGAANGDIPALASDP---NFAQNWVNANVAPFYPASKIILITVGNEVITSNDQNLMNQL 139
            +   N  +PALA      + A++W+ AN++P+ PA+ + L+  GNE++ S D NL+  L
Sbjct: 77  AVSLPNSALPALADKATGLDAARSWIRANLSPYVPATNVTLLLAGNEILLSTDTNLILSL 136

Query: 140 LPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEP-PSSAVFHPNYMSVL-KGLLEFNS 199
           LPA++ L  AL +  L + ++V+T H + +L  S+  PS+A F   Y + L   +L+F+ 
Sbjct: 137 LPAMRRLAQALKAEGL-TGVRVTTPHYLGILAPSDGIPSNASFRAGYNTKLFPAMLQFHR 196

Query: 200 ATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMFDAQVDAIRSAL 259
            TGSPF +NPYPYF+Y    RPETL + LF+PN+G  D  T + Y +M DAQ+DAI +A+
Sbjct: 197 DTGSPFMVNPYPYFSY----RPETLNYALFRPNSGIYDPATKLNYTSMLDAQMDAIYTAM 256

Query: 260 NSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSMVGTPLMPGKSVDT 319
             +G+ +V+I V E GWP + + G++G G++ A+ FN  +I    S  GTPLMP ++ +T
Sbjct: 257 KKLGYGDVDIAVGEAGWPTQAEPGQIGVGVQEARDFNEGMIRVCSSGKGTPLMPNRTFET 316

Query: 320 YLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPKTPATTPSSPANPS 379
           YLF+L+DE+ KPGP +ER FGLF PD T  YD+GL + G S       P  +P+   NPS
Sbjct: 317 YLFSLFDENQKPGPIAERHFGLFNPDFTPVYDLGLLRDGASV-----APTPSPNPSPNPS 376

Query: 380 TKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQPGGACFEPNTIASH 439
            K    P P +GGG WCV   G +   LQ N++YACG  +DC  IQ GGACF PN++ +H
Sbjct: 377 PK----PAP-SGGGKWCVAKDGANGTDLQNNINYACG-FVDCKPIQSGGACFSPNSLQAH 436

Query: 440 AAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNY 473
           A+Y MN ++QA G     CDF  T  ++S++PSY GC Y
Sbjct: 437 ASYVMNAYYQANGHTDLACDFKGTGIVTSSDPSYGGCKY 459

BLAST of MC11g0171 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 4.0e-92
Identity = 194/474 (40.93%), Postives = 281/474 (59.28%), Query Frame = 0

Query: 6   LRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRLYGADP 65
           L    + F++IL   +F  + S IGI YG+ ADNLP P+  ++L+Q  +I+ VR+Y A+ 
Sbjct: 5   LNLVFWIFVSILAFLNFGMA-SKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANI 64

Query: 66  AIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVGNEVIT 125
            ++KA ANTGI ++IG  N D+ A A   +    W++ N+ P+YP++KI  I+VG EV  
Sbjct: 65  DVLKAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTE 124

Query: 126 SNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPNYMSVL 185
           + D N    +LPA++N+  AL    L   IK+S+ HS+A+L +S PPSSA F   + + L
Sbjct: 125 APD-NATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFL 184

Query: 186 KGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMFDAQ 245
           K +LEF     SPF I+ YPY+AY+       L + LF+ ++  +D  T + Y NMFDAQ
Sbjct: 185 KPMLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQ 244

Query: 246 VDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSMVGTPL 305
           +DAI  AL +M FK V+++V E+GWP KG   E     ENA A+N NLI H+    GTP 
Sbjct: 245 LDAIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPA 304

Query: 306 MPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASP--KTPA 365
            PG+ +D YLF+L++E+ KPG  SER +G+F  + T  Y +  +    +TP SP   T  
Sbjct: 305 KPGEEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFT-GENTTPVSPTNSTTG 364

Query: 366 TTPSSPANPSTKAPAGPKPKAGGG----SWCVPMGGVSDAQLQANLDYACGRG-LDCSAI 425
           T+PS  ++P     +      GGG     WC+     S  +LQ  LD+ACG G +DCSA+
Sbjct: 365 TSPSPSSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAV 424

Query: 426 QPGGACFEPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNY 473
           QP   CFEP+T+ SHA+YA N ++Q +G     C F+  +     +PSY  C Y
Sbjct: 425 QPDQPCFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

BLAST of MC11g0171 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 2.8e-85
Identity = 173/465 (37.20%), Postives = 264/465 (56.77%), Query Frame = 0

Query: 10  VFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRLYGADPAIIK 69
           +F    +LL+ D  Y    +G+ YG+ AD+LP PS   +L+Q  +I+ VR+Y  +  ++K
Sbjct: 8   IFSISILLLLLDCCYGGK-VGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLK 67

Query: 70  ALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVGNEVITSNDQ 129
           A  NT I ++IG  N D+ A +   +    W+  +V P+YP +KI  ITVG E       
Sbjct: 68  AFGNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHI 127

Query: 130 NLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPNYMSVLKGLL 189
           N  + ++PA+QN+  AL  + L   IKVST  S+ +L +S PPS+  F+ +Y   L+ +L
Sbjct: 128 NASSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAYFLRPML 187

Query: 190 EFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMFDAQVDAI 249
           EF +   SPF I+ YPY+AY+  P   +L + LF+ ++  +D NT + Y NMFDAQVDA+
Sbjct: 188 EFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDAL 247

Query: 250 RSALNSMGFKNVEIVVAETGWPYKGDGGE-VGPGLENAKAFNGNLIAHLRSMVGTPLMPG 309
             AL ++ F+ ++I+V ETGWP KG   E      +NA+ +N N+I H+ +  GTP  PG
Sbjct: 248 YYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPG 307

Query: 310 KSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPKTPATTPSS 369
           ++++ Y+F+L++E+ K G  SER +GLF PD T  Y   L  +G+S      +  T  S 
Sbjct: 308 EAMNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQ--LDFTGKSNGFHSNSSGTNSSG 367

Query: 370 PANPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRG-LDCSAIQPGGACFEP 429
            +N                SWC+     S+  L+  LD+ACG G +DC+AIQP   CF+P
Sbjct: 368 SSN----------------SWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQP 427

Query: 430 NTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNY 473
           +T+ SHA++  N +FQ        C F       + +PSY+ C Y
Sbjct: 428 DTLVSHASFVFNSYFQQNRATDVACSFGGAGVKVNKDPSYDKCIY 453

BLAST of MC11g0171 vs. NCBI nr
Match: XP_022142141.1 (glucan endo-1,3-beta-glucosidase 7-like [Momordica charantia])

HSP 1 Score: 946 bits (2446), Expect = 0.0
Identity = 477/477 (100.00%), Postives = 477/477 (100.00%), Query Frame = 0

Query: 1   MASTLLRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60
           MASTLLRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL
Sbjct: 1   MASTLLRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVG 120

Query: 121 NEVITSNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPN 180
           NEVITSNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPN
Sbjct: 121 NEVITSNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPN 180

Query: 181 YMSVLKGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YMSVLKGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMSVLKGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPK
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPK 360

Query: 361 TPATTPSSPANPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQP 420
           TPATTPSSPANPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQP
Sbjct: 361 TPATTPSSPANPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQP 420

Query: 421 GGACFEPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGGST 477
           GGACFEPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGGST
Sbjct: 421 GGACFEPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGGST 477

BLAST of MC11g0171 vs. NCBI nr
Match: XP_038905742.1 (glucan endo-1,3-beta-glucosidase 7-like [Benincasa hispida])

HSP 1 Score: 882 bits (2278), Expect = 0.0
Identity = 444/478 (92.89%), Postives = 456/478 (95.40%), Query Frame = 0

Query: 1   MASTLLRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60
           MA+TLLR+TVFFF + LL  D  YSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL
Sbjct: 1   MANTLLRSTVFFFFSFLLATDIVYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFA+NWVNANVAPFYPASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFYPASKIILITVG 120

Query: 121 NEVITSNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPN 180
           NEVITSND+NLMNQL+PAIQNLQNALNS+SLG +IKVSTVHSMAVLRQSEPPSS +FHPN
Sbjct: 121 NEVITSNDENLMNQLVPAIQNLQNALNSMSLGKDIKVSTVHSMAVLRQSEPPSSGMFHPN 180

Query: 181 YMSVLKGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YMSVLKGLLEFN+ATGSPFTINPYPYFAY+SDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDG EVGP LENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGDEVGPSLENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTY+VGLSK GQSTPA  K
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYNVGLSKDGQSTPAGSK 360

Query: 361 TPATTPSSPA-NPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQ 420
           TP TTPSSPA NPSTKAP  PKPKAGGGSWCVP GGVSDAQLQANLDYACGRGLDCSAIQ
Sbjct: 361 TPVTTPSSPATNPSTKAPTTPKPKAGGGSWCVPKGGVSDAQLQANLDYACGRGLDCSAIQ 420

Query: 421 PGGACFEPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGGST 477
           PGGACFEPNTIASHAAYAMNLFFQ A RDPWTCDFS TATLSSNNPSYN CNYPGGST
Sbjct: 421 PGGACFEPNTIASHAAYAMNLFFQNADRDPWTCDFSQTATLSSNNPSYNSCNYPGGST 478

BLAST of MC11g0171 vs. NCBI nr
Match: XP_008466580.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 7 [Cucumis melo] >KAA0041635.1 glucan endo-1,3-beta-glucosidase 7 [Cucumis melo var. makuwa] >TYK19623.1 glucan endo-1,3-beta-glucosidase 7 [Cucumis melo var. makuwa])

HSP 1 Score: 861 bits (2224), Expect = 1.43e-313
Identity = 436/479 (91.02%), Postives = 455/479 (94.99%), Query Frame = 0

Query: 1   MASTLLRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60
           MASTLL +TVF F + LL  D  YSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL
Sbjct: 1   MASTLLPSTVFVFFSFLLATDIVYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALA+DPNFA+NWVNANVAPF+PASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALAADPNFAKNWVNANVAPFHPASKIILITVG 120

Query: 121 NEVITSNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPN 180
           NEVITSN +NLMNQL+PAIQN+QNALNS+SLG +IKVSTVHSMAVLRQSEPPSS +FHPN
Sbjct: 121 NEVITSNQENLMNQLVPAIQNIQNALNSMSLG-DIKVSTVHSMAVLRQSEPPSSGMFHPN 180

Query: 181 YMSVLKGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YM+VLK LLEFN+ATGSPFTINPYPYFAY+SDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMTVLKELLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGD  EVGP LENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDNDEVGPSLENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSK+GQSTP+SPK
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKNGQSTPSSPK 360

Query: 361 T-PATTPSSPAN-PSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAI 420
           T P TTPSSPAN PSTKAP  PKPKAGGGSWC+P GGVSDAQLQANLDYACGRGLDCSAI
Sbjct: 361 TTPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAI 420

Query: 421 QPGGACFEPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGGST 477
           QPGGACFEPNTIASHAAYAMNLFFQ  GRDPWTCDFS +ATLSSNNPSY+GCNYPGGST
Sbjct: 421 QPGGACFEPNTIASHAAYAMNLFFQNGGRDPWTCDFSQSATLSSNNPSYDGCNYPGGST 478

BLAST of MC11g0171 vs. NCBI nr
Match: XP_023525051.1 (glucan endo-1,3-beta-glucosidase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 860 bits (2221), Expect = 3.82e-313
Identity = 431/477 (90.36%), Postives = 454/477 (95.18%), Query Frame = 0

Query: 1   MASTLLRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60
           MA+ LLR+TVFFF+  LL+ + A SQSFIGINYG+VADNLPPPSATAKLLQSTSIEKVRL
Sbjct: 1   MATALLRSTVFFFVYSLLIFNIADSQSFIGINYGEVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFA+NWVNANVAPF PASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFSPASKIILITVG 120

Query: 121 NEVITSNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPN 180
           NEVITSNDQNLMNQL+PAIQNL NALNS+SLG +IKVSTVHSMAVLRQSEPPSSA+FHPN
Sbjct: 121 NEVITSNDQNLMNQLVPAIQNLHNALNSMSLG-DIKVSTVHSMAVLRQSEPPSSALFHPN 180

Query: 181 YMSVLKGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YMSVLK LLEFN+ATGSPFTINPYPYFAY+SDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMSVLKELLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDG EVGP +ENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGDEVGPSVENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYD+GL K+GQ+TPA+PK
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDIGLLKNGQNTPAAPK 360

Query: 361 TPATTPSSPANPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQP 420
           TP TTPSSPANPS KAP  PKPKAGGGSWCVP GGVS+AQLQANLDYACGRG+DCSAIQP
Sbjct: 361 TPVTTPSSPANPSAKAPTSPKPKAGGGSWCVPKGGVSNAQLQANLDYACGRGIDCSAIQP 420

Query: 421 GGACFEPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGGST 477
           GGACFEPNTI +HA YAMNLF+Q AGR PWTCDFS +ATLSSNNPSYNGCNYPGGST
Sbjct: 421 GGACFEPNTIVAHATYAMNLFYQTAGRYPWTCDFSQSATLSSNNPSYNGCNYPGGST 476

BLAST of MC11g0171 vs. NCBI nr
Match: KAG6608530.1 (Glucan endo-1,3-beta-glucosidase 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037853.1 Glucan endo-1,3-beta-glucosidase 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 858 bits (2216), Expect = 2.21e-312
Identity = 430/477 (90.15%), Postives = 452/477 (94.76%), Query Frame = 0

Query: 1   MASTLLRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60
           MA+ LLR+TVFFF+  LL+ + A SQSFIGINYG+VADNLPPPSATAKLLQSTSIEKVRL
Sbjct: 1   MATALLRSTVFFFVYFLLIFNIADSQSFIGINYGEVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFA+NWVNANVAPF PASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFSPASKIILITVG 120

Query: 121 NEVITSNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPN 180
           NEVITSNDQNLMNQL+PAIQNL NALNS+SLG +IKVSTVHSMAVLRQSEPPSSA+FHPN
Sbjct: 121 NEVITSNDQNLMNQLVPAIQNLHNALNSMSLG-DIKVSTVHSMAVLRQSEPPSSALFHPN 180

Query: 181 YMSVLKGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YMSVLK LLEFN+ATGSPFTINPYPYFAY+SDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMSVLKELLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDG EVGP +ENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGNEVGPSVENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYD+GL K GQ+TPA+PK
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDIGLLKHGQNTPAAPK 360

Query: 361 TPATTPSSPANPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQP 420
           TP TTPSSPANPS KAP  PKPKAGGG WCVP GGVS+AQLQANLDYACGRG+DCSAIQP
Sbjct: 361 TPVTTPSSPANPSAKAPTSPKPKAGGGLWCVPKGGVSNAQLQANLDYACGRGIDCSAIQP 420

Query: 421 GGACFEPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGGST 477
           GGACFEPNTI +HA YAMNLF+Q AGR PWTCDFS +ATLSSNNPSYNGCNYPGGST
Sbjct: 421 GGACFEPNTIVAHATYAMNLFYQTAGRYPWTCDFSQSATLSSNNPSYNGCNYPGGST 476

BLAST of MC11g0171 vs. ExPASy TrEMBL
Match: A0A6J1CLC6 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111012336 PE=3 SV=1)

HSP 1 Score: 946 bits (2446), Expect = 0.0
Identity = 477/477 (100.00%), Postives = 477/477 (100.00%), Query Frame = 0

Query: 1   MASTLLRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60
           MASTLLRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL
Sbjct: 1   MASTLLRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVG 120

Query: 121 NEVITSNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPN 180
           NEVITSNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPN
Sbjct: 121 NEVITSNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPN 180

Query: 181 YMSVLKGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YMSVLKGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMSVLKGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPK
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPK 360

Query: 361 TPATTPSSPANPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQP 420
           TPATTPSSPANPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQP
Sbjct: 361 TPATTPSSPANPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQP 420

Query: 421 GGACFEPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGGST 477
           GGACFEPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGGST
Sbjct: 421 GGACFEPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGGST 477

BLAST of MC11g0171 vs. ExPASy TrEMBL
Match: A0A5A7TJN0 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold242G00090 PE=3 SV=1)

HSP 1 Score: 861 bits (2224), Expect = 6.92e-314
Identity = 436/479 (91.02%), Postives = 455/479 (94.99%), Query Frame = 0

Query: 1   MASTLLRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60
           MASTLL +TVF F + LL  D  YSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL
Sbjct: 1   MASTLLPSTVFVFFSFLLATDIVYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALA+DPNFA+NWVNANVAPF+PASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALAADPNFAKNWVNANVAPFHPASKIILITVG 120

Query: 121 NEVITSNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPN 180
           NEVITSN +NLMNQL+PAIQN+QNALNS+SLG +IKVSTVHSMAVLRQSEPPSS +FHPN
Sbjct: 121 NEVITSNQENLMNQLVPAIQNIQNALNSMSLG-DIKVSTVHSMAVLRQSEPPSSGMFHPN 180

Query: 181 YMSVLKGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YM+VLK LLEFN+ATGSPFTINPYPYFAY+SDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMTVLKELLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGD  EVGP LENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDNDEVGPSLENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSK+GQSTP+SPK
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKNGQSTPSSPK 360

Query: 361 T-PATTPSSPAN-PSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAI 420
           T P TTPSSPAN PSTKAP  PKPKAGGGSWC+P GGVSDAQLQANLDYACGRGLDCSAI
Sbjct: 361 TTPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAI 420

Query: 421 QPGGACFEPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGGST 477
           QPGGACFEPNTIASHAAYAMNLFFQ  GRDPWTCDFS +ATLSSNNPSY+GCNYPGGST
Sbjct: 421 QPGGACFEPNTIASHAAYAMNLFFQNGGRDPWTCDFSQSATLSSNNPSYDGCNYPGGST 478

BLAST of MC11g0171 vs. ExPASy TrEMBL
Match: A0A1S3CRM8 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103503956 PE=3 SV=1)

HSP 1 Score: 861 bits (2224), Expect = 6.92e-314
Identity = 436/479 (91.02%), Postives = 455/479 (94.99%), Query Frame = 0

Query: 1   MASTLLRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60
           MASTLL +TVF F + LL  D  YSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL
Sbjct: 1   MASTLLPSTVFVFFSFLLATDIVYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALA+DPNFA+NWVNANVAPF+PASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALAADPNFAKNWVNANVAPFHPASKIILITVG 120

Query: 121 NEVITSNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPN 180
           NEVITSN +NLMNQL+PAIQN+QNALNS+SLG +IKVSTVHSMAVLRQSEPPSS +FHPN
Sbjct: 121 NEVITSNQENLMNQLVPAIQNIQNALNSMSLG-DIKVSTVHSMAVLRQSEPPSSGMFHPN 180

Query: 181 YMSVLKGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YM+VLK LLEFN+ATGSPFTINPYPYFAY+SDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMTVLKELLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGD  EVGP LENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDNDEVGPSLENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSK+GQSTP+SPK
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKNGQSTPSSPK 360

Query: 361 T-PATTPSSPAN-PSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAI 420
           T P TTPSSPAN PSTKAP  PKPKAGGGSWC+P GGVSDAQLQANLDYACGRGLDCSAI
Sbjct: 361 TTPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAI 420

Query: 421 QPGGACFEPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGGST 477
           QPGGACFEPNTIASHAAYAMNLFFQ  GRDPWTCDFS +ATLSSNNPSY+GCNYPGGST
Sbjct: 421 QPGGACFEPNTIASHAAYAMNLFFQNGGRDPWTCDFSQSATLSSNNPSYDGCNYPGGST 478

BLAST of MC11g0171 vs. ExPASy TrEMBL
Match: A0A6J1J4P1 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111481285 PE=3 SV=1)

HSP 1 Score: 857 bits (2215), Expect = 1.52e-312
Identity = 429/477 (89.94%), Postives = 453/477 (94.97%), Query Frame = 0

Query: 1   MASTLLRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60
           MA+ LLR+T FFF+  LL+ + A SQSFIGINYG+VADNLPPPSATAKLLQSTSIEKVRL
Sbjct: 1   MATALLRSTAFFFVYFLLIFNIADSQSFIGINYGEVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFA+NWVNANVAPF PASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFSPASKIILITVG 120

Query: 121 NEVITSNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPN 180
           NEVITSNDQNLMNQL+PAIQNL NALNS+SLG +IKVSTVHSMAVLRQSEPPSSA+FHPN
Sbjct: 121 NEVITSNDQNLMNQLVPAIQNLHNALNSMSLG-DIKVSTVHSMAVLRQSEPPSSALFHPN 180

Query: 181 YMSVLKGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YMSVLKGLLEFN+ATGSPFTINPYPYFAY+SDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKG+G EVGP +ENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGNGDEVGPSVENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYD+GL K+GQ+TPA+PK
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDIGLLKNGQNTPAAPK 360

Query: 361 TPATTPSSPANPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQP 420
           TP TTPSSPANPS KAP  PKP AGGGSWCVP GGVS+AQLQANLDYACGRG+DCSAIQP
Sbjct: 361 TPVTTPSSPANPSAKAPTSPKPNAGGGSWCVPKGGVSNAQLQANLDYACGRGIDCSAIQP 420

Query: 421 GGACFEPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGGST 477
           GGACFEPNTIA+HA YAMNLF+Q AGR PWTCDFS +ATLSS NPSYNGCNYPGGST
Sbjct: 421 GGACFEPNTIAAHATYAMNLFYQTAGRYPWTCDFSQSATLSSKNPSYNGCNYPGGST 476

BLAST of MC11g0171 vs. ExPASy TrEMBL
Match: A0A0A0LG31 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G855430 PE=3 SV=1)

HSP 1 Score: 852 bits (2202), Expect = 1.56e-310
Identity = 431/479 (89.98%), Postives = 450/479 (93.95%), Query Frame = 0

Query: 1   MASTLLRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60
           MAST+L +T+F F   LL  +  YSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL
Sbjct: 1   MASTILPSTIFVFFTFLLATEIVYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALA+DPNFA+NWVNANVAPF+PASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALAADPNFAKNWVNANVAPFHPASKIILITVG 120

Query: 121 NEVITSNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPN 180
           NEVITSN  NLMNQL+PAIQN+QNALNS+SLG +IKVSTVHSMAVLRQSEPPSS +FHPN
Sbjct: 121 NEVITSNQDNLMNQLVPAIQNIQNALNSMSLG-DIKVSTVHSMAVLRQSEPPSSGMFHPN 180

Query: 181 YMSVLKGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YM+VLK LLEFN+ATGSPFTINPYPYFAY+SDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMTVLKELLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGD  EVG  LENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDNDEVGSSLENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTM YDVGLSK+GQSTP SPK
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMAYDVGLSKNGQSTPTSPK 360

Query: 361 T-PATTPSSPAN-PSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAI 420
           T P TTPSSPAN PSTK+P  PKPKAGGGSWC+P GGVSDAQLQANLDYACGRGLDCSAI
Sbjct: 361 TTPVTTPSSPANNPSTKSPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAI 420

Query: 421 QPGGACFEPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGGST 477
           QPGGACFEPNTIASHAAYAMNLFFQ  GRDPWTCDFS +ATLSSNNPSYNGCNYPGGST
Sbjct: 421 QPGGACFEPNTIASHAAYAMNLFFQNGGRDPWTCDFSQSATLSSNNPSYNGCNYPGGST 478

BLAST of MC11g0171 vs. TAIR 10
Match: AT4G34480.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 635.2 bits (1637), Expect = 4.3e-182
Identity = 312/470 (66.38%), Postives = 367/470 (78.09%), Query Frame = 0

Query: 6   LRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRLYGADP 65
           L  +++F L  L     ++++ FIG+NYGQVADNLPPPS T KLLQSTSI+KVRLYGADP
Sbjct: 3   LSISIYFLLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADP 62

Query: 66  AIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVGNEVIT 125
           AIIKALA TG+GIVIGAANGD+P+LASDPN A  W+N+NV PFYPASKI+LITVGNE++ 
Sbjct: 63  AIIKALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILM 122

Query: 126 SNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPNYMSVL 185
           SND NL+NQLLPA+QN+Q AL ++SLG  IKVSTV+SM VL  S+PPSS  F   Y + L
Sbjct: 123 SNDPNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGL 182

Query: 186 KGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMFDAQ 245
           KG+L+F S TGSPF INPYP+FAYQSDPRPETLAFCLF+PNAGR+D+ T IKY NMFDAQ
Sbjct: 183 KGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQ 242

Query: 246 VDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSMVGTPL 305
           VDA+ SAL SMGF+ VEIVVAETGW  +GD  EVG  ++NAKA+NGNLIAHLRSMVGTPL
Sbjct: 243 VDAVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPL 302

Query: 306 MPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPKTPATT 365
           MPGK VDTY+FALYDE+LKPGP SERAFGLFK DL+M YDVGL+KS  S+          
Sbjct: 303 MPGKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAKSSSSSQT-------- 362

Query: 366 PSSPANPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQPGGACF 425
                         P  K     WCVP  G ++ +LQA+LD+ACG G+DC AIQPGGACF
Sbjct: 363 --------------PSGKVTSSGWCVPKKGATNEELQASLDWACGHGIDCGAIQPGGACF 422

Query: 426 EPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGG 476
           EPN + SHAAYAMN++FQ + + P  CDFS TAT++S NPSYN C YPGG
Sbjct: 423 EPNNVVSHAAYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYPGG 450

BLAST of MC11g0171 vs. TAIR 10
Match: AT2G16230.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 599.0 bits (1543), Expect = 3.4e-171
Identity = 296/464 (63.79%), Postives = 355/464 (76.51%), Query Frame = 0

Query: 10  VFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRLYGADPAIIK 69
           +F  L IL     + +QSFIG+NYG ++DNLPPPS TAKLLQSTSI+KVRLY AD +II 
Sbjct: 6   LFLLLFILFSISPSNAQSFIGVNYGLLSDNLPPPSQTAKLLQSTSIQKVRLYNADSSIIT 65

Query: 70  ALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVGNEVITSNDQ 129
           +L  TGIGIVIG ANGD+P++ASD N A  W+N+NV PFYPAS IILI VGNEV+ SND 
Sbjct: 66  SLVGTGIGIVIGVANGDLPSIASDLNIASQWINSNVLPFYPASNIILINVGNEVLLSNDL 125

Query: 130 NLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPNYMSVLKGLL 189
           NL+NQLLPA+QN+Q AL ++SLG  IKVSTVH+M VL  SEPPS+  F P+Y + LKG+L
Sbjct: 126 NLVNQLLPAMQNVQKALEAVSLGGKIKVSTVHAMTVLGNSEPPSAGSFAPSYQAGLKGIL 185

Query: 190 EFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMFDAQVDAI 249
           +F S TGSPF INPYP+FAYQSDPRPETLAFCLFQPN GR+D+NT IKYMNMFDAQVDA+
Sbjct: 186 QFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFQPNPGRVDSNTGIKYMNMFDAQVDAV 245

Query: 250 RSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSMVGTPLMPGK 309
            SAL S+GF+ VE++VAETGWP  GD  EVGP +ENAKA+NGNLIAHLRSMVGTPLMPGK
Sbjct: 246 HSALKSIGFEKVEVLVAETGWPSTGDSNEVGPSVENAKAYNGNLIAHLRSMVGTPLMPGK 305

Query: 310 SVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPKTPATTPSSP 369
           S+DTY+FAL+DE+LKPGP  E++FGLFKPDL+M YD+GL+K+         T + T  SP
Sbjct: 306 SIDTYIFALFDENLKPGPSFEQSFGLFKPDLSMAYDIGLTKT---------TSSQTSQSP 365

Query: 370 ANPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQPGGACFEPNT 429
                        K     WCVP    +  QLQ +LD+ CG+G+DC  I PGG CFEPN 
Sbjct: 366 ----------QLGKVTSMGWCVPKEDATQEQLQDSLDWVCGQGIDCGPIMPGGVCFEPNN 425

Query: 430 IASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYP 474
           +ASH AYAMNL+FQ +  +P  CDFS TA ++S NPSY+ C YP
Sbjct: 426 VASHTAYAMNLYFQKSPENPTDCDFSKTARITSENPSYSSCVYP 450

BLAST of MC11g0171 vs. TAIR 10
Match: AT5G42720.1 (Glycosyl hydrolase family 17 protein )

HSP 1 Score: 486.9 bits (1252), Expect = 1.9e-137
Identity = 250/398 (62.81%), Postives = 306/398 (76.88%), Query Frame = 0

Query: 6   LRATVFFFLNILL--VADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRLYGA 65
           +RA+V+  + +    +   + SQ F+G+NYG  ADNLPPPSA+AKLLQST+ +KVRLYG+
Sbjct: 1   MRASVYSLILLFFSCLLHLSKSQPFLGVNYGLTADNLPPPSASAKLLQSTTFQKVRLYGS 60

Query: 66  DPAIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVGNEV 125
           DPA+IKALANTGI IVIGA+NGD+P LASDP+FA++WV  NV P+YPASKI+LI VGNE+
Sbjct: 61  DPAVIKALANTGIEIVIGASNGDVPGLASDPSFARSWVETNVVPYYPASKIVLIAVGNEI 120

Query: 126 ITSNDQNLMNQLLPAIQNLQNALNSISL-GSNIKVSTVHSMAVLRQSEPPSSAVFHPNYM 185
            +  D +LM+QLLPA++N+Q AL + SL G  IKVSTVH M+VL  S+PPS+AVF P + 
Sbjct: 121 TSFGDNSLMSQLLPAMKNVQTALEAASLGGGKIKVSTVHIMSVLAGSDPPSTAVFKPEHA 180

Query: 186 SVLKGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMF 245
            +LKGLLEFNS TGSPF +NPYP+FAYQ D RPETLA+CLFQ N GR+D N+N+KYMNMF
Sbjct: 181 DILKGLLEFNSETGSPFAVNPYPFFAYQDDRRPETLAYCLFQANPGRVDPNSNLKYMNMF 240

Query: 246 DAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSMVG 305
           DAQVDA+ SALNSMGFK+VEI+VAETGWPYKGD  E G  +ENA+A+N NLIAHL+S  G
Sbjct: 241 DAQVDAVYSALNSMGFKDVEIMVAETGWPYKGDPEEAGATVENARAYNKNLIAHLKSGSG 300

Query: 306 TPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKS-----GQSTPA 365
           TPLMPG+ +DTYLFALYDE+LKPG GSERAFGLF+PDLTMTYD+GL+K+         P 
Sbjct: 301 TPLMPGRVIDTYLFALYDENLKPGKGSERAFGLFRPDLTMTYDIGLTKTTNYNQTSMAPL 360

Query: 366 SPKTPATTPS---------SPANPSTKAPAGPKPKAGG 387
           SP  P   P+         SP      +P  P  K  G
Sbjct: 361 SPTRPRLPPAAAPTRQTLPSPPQMILPSPVTPSDKNSG 398

BLAST of MC11g0171 vs. TAIR 10
Match: AT5G24318.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 385.2 bits (988), Expect = 7.7e-107
Identity = 201/466 (43.13%), Postives = 289/466 (62.02%), Query Frame = 0

Query: 9   TVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAK-LLQSTSIEKVRLYGADPAI 68
           ++F  + +LL+     S++ IG+NYG +A+NLPPP   A+ LL ST I ++RL+  DP I
Sbjct: 11  SMFTIVGVLLILSTG-SEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDTDPQI 70

Query: 69  IKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVGNEVITSN 128
           ++A ANTGI + +   N  IP L ++ + A+ W++ ++ P +P++ II I VGNEVI++ 
Sbjct: 71  LQAFANTGIAVTVTVPNDQIPHL-TNLSSAKQWISDHIQPHFPSTNIIRILVGNEVISTA 130

Query: 129 DQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPNY-MSVLK 188
           D  L+  L+PA+Q+L  AL S SL   I++ST HS+  L  S PPSSA F   Y   VLK
Sbjct: 131 DHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQVLK 190

Query: 189 GLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMFDAQV 248
            LL F  +T SPF +NPYP+F Y      ETL F LF+PN G  D +T + Y NM DAQ+
Sbjct: 191 PLLSFLRSTSSPFVVNPYPFFGYSI----ETLDFALFRPNPGLFDQHTKLLYTNMLDAQL 250

Query: 249 DAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSMVGTPLM 308
           D++ SA++ +GF +VEIV+ E GWP +GD  ++G  ++ A  FN NLIA + S  GTPLM
Sbjct: 251 DSVYSAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLM 310

Query: 309 PGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPKTPATTP 368
           P ++ +TY+FAL++E+LK GP SER FG+F+ DLT  YD+G+ +               P
Sbjct: 311 PNRTFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILR---------------P 370

Query: 369 SSPANPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQPGGACFE 428
           +  ++     P  P   +    WCV   G     LQ N+DY CG GLDC  I  GG C+ 
Sbjct: 371 TFRSSDPVYNPRSPVRGSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYL 430

Query: 429 PNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNY 473
           PNT+ +H+ YAMNL++Q   +  + CDF +T  +++ +PSY  C Y
Sbjct: 431 PNTVKAHSKYAMNLYYQTMEKHEFDCDFDNTGEITTIDPSYGNCEY 455

BLAST of MC11g0171 vs. TAIR 10
Match: AT5G24318.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 378.6 bits (971), Expect = 7.2e-105
Identity = 199/462 (43.07%), Postives = 287/462 (62.12%), Query Frame = 0

Query: 9   TVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAK-LLQSTSIEKVRLYGADPAI 68
           ++F  + +LL+     S++ IG+NYG +A+NLPPP   A+ LL ST I ++RL+  DP I
Sbjct: 11  SMFTIVGVLLILSTG-SEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDTDPQI 70

Query: 69  IKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVGNEVITSN 128
           ++A ANTGI + +   N  IP L ++ + A+ W++ ++ P +P++ II I VGNEVI++ 
Sbjct: 71  LQAFANTGIAVTVTVPNDQIPHL-TNLSSAKQWISDHIQPHFPSTNIIRILVGNEVISTA 130

Query: 129 DQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPNY-MSVLK 188
           D  L+  L+PA+Q+L  AL S SL   I++ST HS+  L  S PPSSA F   Y   VLK
Sbjct: 131 DHLLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQVLK 190

Query: 189 GLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMFDAQV 248
            LL F  +T SPF +NPYP+F Y      ETL F LF+PN G  D +T + Y NM DAQ+
Sbjct: 191 PLLSFLRSTSSPFVVNPYPFFGYSI----ETLDFALFRPNPGLFDQHTKLLYTNMLDAQL 250

Query: 249 DAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSMVGTPLM 308
           D++ SA++ +GF +VEIV+ E GWP +GD  ++G  ++ A  FN NLIA + S  GTPLM
Sbjct: 251 DSVYSAMDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLM 310

Query: 309 PGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPKTPATTP 368
           P ++ +TY+FAL++E+LK GP SER FG+F+ DLT  YD+G+ +               P
Sbjct: 311 PNRTFETYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILR---------------P 370

Query: 369 SSPANPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQPGGACFE 428
           +  ++     P  P   +    WCV   G     LQ N+DY CG GLDC  I  GG C+ 
Sbjct: 371 TFRSSDPVYNPRSPVRGSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYL 430

Query: 429 PNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYN 469
           PNT+ +H+ YAMNL++Q   +  + CDF +T  +++ +PS N
Sbjct: 431 PNTVKAHSKYAMNLYYQTMEKHEFDCDFDNTGEITTIDPSMN 451

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M0696.0e-18166.38Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q94G863.7e-17063.00Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
P524091.0e-10343.57Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1[more]
Q8VYE54.0e-9240.93Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Q9FJU92.8e-8537.20Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590... [more]
Match NameE-valueIdentityDescription
XP_022142141.10.0100.00glucan endo-1,3-beta-glucosidase 7-like [Momordica charantia][more]
XP_038905742.10.092.89glucan endo-1,3-beta-glucosidase 7-like [Benincasa hispida][more]
XP_008466580.11.43e-31391.02PREDICTED: glucan endo-1,3-beta-glucosidase 7 [Cucumis melo] >KAA0041635.1 gluca... [more]
XP_023525051.13.82e-31390.36glucan endo-1,3-beta-glucosidase 7-like [Cucurbita pepo subsp. pepo][more]
KAG6608530.12.21e-31290.15Glucan endo-1,3-beta-glucosidase 7, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
A0A6J1CLC60.0100.00(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111012336 ... [more]
A0A5A7TJN06.92e-31491.02(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3CRM86.92e-31491.02(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103503956 PE=3 SV... [more]
A0A6J1J4P11.52e-31289.94(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111481285 PE=... [more]
A0A0A0LG311.56e-31089.98(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G855430 PE=3... [more]
Match NameE-valueIdentityDescription
AT4G34480.14.3e-18266.38O-Glycosyl hydrolases family 17 protein [more]
AT2G16230.13.4e-17163.79O-Glycosyl hydrolases family 17 protein [more]
AT5G42720.11.9e-13762.81Glycosyl hydrolase family 17 protein [more]
AT5G24318.17.7e-10743.13O-Glycosyl hydrolases family 17 protein [more]
AT5G24318.27.2e-10543.07O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 388..472
e-value: 1.1E-44
score: 164.5
IPR012946X8 domainPFAMPF07983X8coord: 389..459
e-value: 1.6E-19
score: 70.4
NoneNo IPR availableGENE3D1.20.58.1040coord: 387..472
e-value: 9.4E-9
score: 37.5
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 28..355
e-value: 3.0E-124
score: 416.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 347..361
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 347..384
NoneNo IPR availablePANTHERPTHR32227:SF16GLYCOSYL HYDROLASE FAMILY 17 PROTEINcoord: 5..463
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 29..346
e-value: 1.7E-81
score: 274.2
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 5..463
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 29..347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC11g0171.1MC11g0171.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds