MC11g0154 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g0154 vs. ExPASy Swiss-Prot
Match: Q9FJZ3 (Mannan endo-1,4-beta-mannosidase 7 OS=Arabidopsis thaliana OX=3702 GN=MAN7 PE=2 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 2.7e-156
Identity = 266/417 (63.79%), Postives = 332/417 (79.62%), Query Frame = 0

Query: 3   MKLISRFLVVLLLIVLR-----SEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFA 62
           MKL++ F  + ++I L      ++A    GFVRT+G Q  LNG P+YANGFNAYWLMY A
Sbjct: 1   MKLLALFPFLAIVIQLSCWELGTDALPSGGFVRTKGVQFSLNGYPYYANGFNAYWLMYVA 60

Query: 63  SDPSQRTKVSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAG 122
           SDPSQR+K+S+AFQ+A  HGL++ RTWAFSDGGY  LQYSPG YNE MFQGLD+ ++EA 
Sbjct: 61  SDPSQRSKISTAFQDASRHGLTVARTWAFSDGGYRALQYSPGSYNEDMFQGLDFALAEAR 120

Query: 123 KHGIKLILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMR 182
           +HGIK+ILS  NNYE+ GG+KQYV+WARS+G+ +SSEDDFFT+S+VK FYKNHIK+VL R
Sbjct: 121 RHGIKIILSFANNYESFGGRKQYVDWARSRGRPVSSEDDFFTDSLVKDFYKNHIKAVLNR 180

Query: 183 TNSLTGIAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLE 242
            N+ T + YKDDPTIMAWELMNE RCPSDPSG  IQAWI EMA+++KS+D  HLLEAGLE
Sbjct: 181 FNTFTKVHYKDDPTIMAWELMNEPRCPSDPSGRAIQAWITEMAAHVKSLDRNHLLEAGLE 240

Query: 243 GFYGQSRNQG---NPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTW 302
           GFYGQS  Q    NP  Q GT+FIANN+IP +DF TVHSYPD+W   SS ++Q+ FLN W
Sbjct: 241 GFYGQSSPQSKTLNPPGQFGTDFIANNRIPGIDFVTVHSYPDEWFPDSSEQSQMDFLNKW 300

Query: 303 LNDHIQDAQNILHKPVLFAEFGKSTKYSG--SDQRDQLFNAVYSAVYSSARTGGAAVGGL 362
           L+ HIQDAQN+LHKP++ AEFGKS K  G    QRD +FN VYS +Y SA+ GGAA GGL
Sbjct: 301 LDAHIQDAQNVLHKPIILAEFGKSMKKPGYTPAQRDIVFNTVYSKIYGSAKRGGAAAGGL 360

Query: 363 FWQLLVEGMDSFRDGYEVVLNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAK 410
           FWQLLV G+D+F+DGY ++L+++ ST N+ISQ+S+KL  IRK++A++ N+EK KRA+
Sbjct: 361 FWQLLVNGIDNFQDGYGIILSQSSSTVNVISQQSRKLTLIRKIFARMINVEKWKRAR 417

BLAST of MC11g0154 vs. ExPASy Swiss-Prot
Match: Q0JKM9 (Mannan endo-1,4-beta-mannosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN1 PE=2 SV=2)

HSP 1 Score: 500.7 bits (1288), Expect = 1.6e-140
Identity = 246/407 (60.44%), Postives = 309/407 (75.92%), Query Frame = 0

Query: 10  LVVLLLIVLRSEAEAE-----DGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQRT 69
           L  ++++V+    EAE      GFVR +G + +L+G+P+YANGFNAYWLM  A+DPSQR 
Sbjct: 14  LACVVVVVIHGLGEAEALGGGGGFVRAQGTRFVLDGNPYYANGFNAYWLMLLAADPSQRG 73

Query: 70  KVSSAFQEALNHGLSIGRTWAFSD-GGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIKL 129
           KVS+A  EA  HGL++ RTWAFSD GG N LQ SPG YNE  F+GLD+V+SEA K+GIK+
Sbjct: 74  KVSAALGEAAGHGLTVARTWAFSDGGGGNALQLSPGNYNENTFKGLDFVLSEARKYGIKV 133

Query: 130 ILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLTG 189
           ILSLV+NY++ GG+KQYV WAR+QGQ I S+D+FFTN VVKGFYKNH+K+VL R N++TG
Sbjct: 134 ILSLVDNYDSFGGRKQYVNWARAQGQGIGSDDEFFTNPVVKGFYKNHVKTVLTRKNTITG 193

Query: 190 IAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQS 249
           +AY+DDPTI+AWELMNE RC SD SG T+Q+WI EMA+++KSID  H+LE GLEGFYG S
Sbjct: 194 VAYRDDPTILAWELMNEPRCQSDLSGRTVQSWITEMAAHVKSIDRNHMLEVGLEGFYGAS 253

Query: 250 RNQG----NPN-FQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDH 309
                   NP+ +Q+GT+FIANNQ+P +DFATVHSYPDQWLSG   + QL F+  WL+ H
Sbjct: 254 SPSRIAAVNPSGYQLGTDFIANNQVPGIDFATVHSYPDQWLSGKDDQAQLGFMGRWLDAH 313

Query: 310 IQDAQNILHKPVLFAEFGKSTKYSG--SDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQL 369
           I DAQ +L KP+L AEFGKS K  G  S QRD L+  VY+ +Y SAR GGA VGGLFWQL
Sbjct: 314 IADAQAVLRKPLLIAEFGKSWKDPGYSSGQRDALYGTVYAKIYESARRGGATVGGLFWQL 373

Query: 370 LVEGMDSFRDGYEVVLNENLSTANLISQESQKLIHIRKMYAKLRNIE 404
           LV GMDS+RDGYEVV  E  ST  +I+  S++L  + K +A+ R  +
Sbjct: 374 LVPGMDSYRDGYEVVFGETPSTTGVITTNSRRLRFLSKAFARARQAQ 420

BLAST of MC11g0154 vs. ExPASy Swiss-Prot
Match: Q6Z310 (Putative mannan endo-1,4-beta-mannosidase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN9 PE=2 SV=2)

HSP 1 Score: 450.3 bits (1157), Expect = 2.5e-125
Identity = 219/399 (54.89%), Postives = 284/399 (71.18%), Query Frame = 0

Query: 1   RKMKLISRFLVVLLLI---VLRSEAEAED----GFVRTRGQQLILNGSPFYANGFNAYWL 60
           R++ L+    VV+L I   VL    EA D     F R  G +  + G PFY+NGFNAYWL
Sbjct: 5   RRVILLPTLGVVVLAIAAAVLLHAGEAADVANGQFARASGTRFTVGGRPFYSNGFNAYWL 64

Query: 61  MYFASDPSQRTKVSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVI 120
           MY ASDP  R+K +   Q+A +   ++ RTWAFSDGGY PLQ SPG YNE MF GLD+VI
Sbjct: 65  MYMASDPGDRSKAAGVLQQAASLRATLVRTWAFSDGGYRPLQKSPGVYNEDMFMGLDFVI 124

Query: 121 SEAGKHGIKLILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKS 180
           +EA K G+ LILSLVNN++  GGKKQYV+WAR QG  + S+DDFF + V K FYKNH  +
Sbjct: 125 AEAKKRGLYLILSLVNNWDGFGGKKQYVQWARDQGHNLGSDDDFFRSDVTKQFYKNH--A 184

Query: 181 VLMRTNSLTGIAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLE 240
           VL R N +TG+AYKDDPTI AWEL+NE RC SD SG T+QAW+ EMA Y+KS+D  H++E
Sbjct: 185 VLTRVNKITGVAYKDDPTIFAWELINEPRCQSDLSGKTLQAWVTEMAGYVKSVDPNHMVE 244

Query: 241 AGLEGFYGQSRNQG-NPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLN 300
            GLEGFYG+S ++  NP + VGT+FIANN +P +DFAT+HSYPDQW+SG+S + Q+ F+ 
Sbjct: 245 IGLEGFYGESMHKNFNPGYTVGTDFIANNLVPAVDFATIHSYPDQWVSGASSDEQVAFMR 304

Query: 301 TWLNDHIQDAQNILHKPVLFAEFGKSTKYSGSD--QRDQLFNAVYSAVYSSARTGGAAVG 360
            W+ DHI+D+  +L KP+L  EFG S + +G     RD  F  VY AVY+SAR GGA  G
Sbjct: 305 KWMADHIRDSAAVLRKPLLVTEFGWSARSNGYTVAARDAYFRTVYDAVYASAREGGACAG 364

Query: 361 GLFWQLLVEGMDSFRDGYEVVLNENLSTANLISQESQKL 390
           GLFWQ++  GM+S+ DGYEVVL  + STA++++ +  ++
Sbjct: 365 GLFWQVMAPGMESWTDGYEVVLERSKSTADVVAHQCARI 401

BLAST of MC11g0154 vs. ExPASy Swiss-Prot
Match: Q9FZ03 (Mannan endo-1,4-beta-mannosidase 2 OS=Solanum lycopersicum OX=4081 GN=MAN2 PE=2 SV=2)

HSP 1 Score: 442.2 bits (1136), Expect = 6.7e-123
Identity = 218/400 (54.50%), Postives = 286/400 (71.50%), Query Frame = 0

Query: 4   KLISRFLVVLLLI--------VLRSEAEAED-GFVRTRGQQLILNGSPFYANGFNAYWLM 63
           +LIS   V+ LL         VL  E  A D GFVR  G    LNGSPF  NGFN+YWLM
Sbjct: 6   RLISCIFVLFLLSLAFACEARVLLDENNANDQGFVRVNGAHFELNGSPFLFNGFNSYWLM 65

Query: 64  YFASDPSQRTKVSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVIS 123
           + A++PS+R KVS   +EA + GLS+ RTWAFSDGG   LQ SPG Y+E++FQGLD+VIS
Sbjct: 66  HVAAEPSERYKVSEVLREASSAGLSVCRTWAFSDGGDRALQISPGVYDERVFQGLDFVIS 125

Query: 124 EAGKHGIKLILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSV 183
           EA K+GI+LILS VNNY + GGK QYV+WAR+ G  I+ +DDF+TN + K +YKNHIK V
Sbjct: 126 EAKKYGIRLILSFVNNYNDFGGKAQYVQWARNAGAQINGDDDFYTNYITKNYYKNHIKKV 185

Query: 184 LMRTNSLTGIAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEA 243
           + R N++TG+ YKDD TIMAWELMNE R  +D SGNT+ AW++EMAS++KS+D KHLLE 
Sbjct: 186 VTRFNTITGMTYKDDSTIMAWELMNEPRNQADYSGNTLNAWVQEMASFVKSLDNKHLLEI 245

Query: 244 GLEGFYGQS---RNQGNPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFL 303
           G+EGFYG S   R   NP +QVGT+FI+N+ I E+DFAT+H+Y DQWLSG S + Q+ F+
Sbjct: 246 GMEGFYGDSVPERKSINPGYQVGTDFISNHLIKEIDFATIHAYTDQWLSGQSDDAQMIFM 305

Query: 304 NTWLNDHIQDAQNILHKPVLFAEFGKSTKYSGSDQ--RDQLFNAVYSAVYSSARTGGAAV 363
             W+  H QDA+NIL KP++ AEFGKS++  G +Q  RD   + +Y  +YS A+ GG   
Sbjct: 306 QKWMTSHWQDAKNILKKPLVLAEFGKSSRDPGYNQNIRDTFMSTIYRNIYSLAKDGGTMG 365

Query: 364 GGLFWQLLVEGMDSFRDGYEVVLNENLSTANLISQESQKL 390
           G L WQL+ +GM+++ DGY + L +N STA +I+ +S  +
Sbjct: 366 GSLIWQLVAQGMENYEDGYCIELGKNPSTAGIITSQSHAM 405

BLAST of MC11g0154 vs. ExPASy Swiss-Prot
Match: Q8L5J1 (Mannan endo-1,4-beta-mannosidase 4 OS=Solanum lycopersicum OX=4081 GN=MAN4 PE=1 SV=2)

HSP 1 Score: 442.2 bits (1136), Expect = 6.7e-123
Identity = 217/389 (55.78%), Postives = 279/389 (71.72%), Query Frame = 0

Query: 10  LVVLLLIVLRSE------AEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQR 69
           + V +LI+  +E      A + + FV T G    LNG   Y NGFNAYWLMY A DPS R
Sbjct: 8   IFVAILIIFPNEFSKPTRAFSNNNFVYTDGTHFALNGKSLYINGFNAYWLMYIAYDPSTR 67

Query: 70  TKVSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIKL 129
            KV++ FQ+A  + +++ RTWAFS GG  PLQ +PG YNEQMFQGLD+VISEA K+GI L
Sbjct: 68  IKVTNTFQQASKYKMNVARTWAFSHGGSRPLQSAPGVYNEQMFQGLDFVISEAKKYGIHL 127

Query: 130 ILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLTG 189
           I+SLVNN++  GGKKQYVEWA  +GQ ++S+DDFFTN +VKGFYKN++K VL R N++T 
Sbjct: 128 IMSLVNNWDAFGGKKQYVEWAVQRGQKLTSDDDFFTNPMVKGFYKNNVKVVLTRVNTITK 187

Query: 190 IAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQS 249
           +AYKDDPTI++WEL+NE RCPSD SG T Q W+ EMA YLKSID  HLLE GLEGFYG  
Sbjct: 188 VAYKDDPTILSWELINEPRCPSDLSGKTFQNWVLEMAGYLKSIDSNHLLEIGLEGFYGND 247

Query: 250 RNQGNPNFQV-GTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDA 309
             Q NPN  + GTNFI+NNQ+  +DF T+H YP+QWL G + E Q  + + W+  HI D+
Sbjct: 248 MRQYNPNSYIFGTNFISNNQVQGIDFTTIHMYPNQWLPGLTQEAQDKWASQWIQVHIDDS 307

Query: 310 QNILHKPVLFAEFGKSTKYSGSD--QRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEG 369
           + +L KP+L AEFGKSTK  G    +RD  F  +Y  +++ A++GG   GGLFWQ+L +G
Sbjct: 308 K-MLKKPLLIAEFGKSTKTPGYTVAKRDNYFEKIYGTIFNCAKSGGPCGGGLFWQVLGQG 367

Query: 370 MDSFRDGYEVVLNENLSTANLISQESQKL 390
           M SF DGY+VVL E+ ST+ +I  +S +L
Sbjct: 368 MSSFDDGYQVVLQESPSTSRVILLQSLRL 395

BLAST of MC11g0154 vs. NCBI nr
Match: XP_022132099.1 (mannan endo-1,4-beta-mannosidase 7-like [Momordica charantia])

HSP 1 Score: 854 bits (2206), Expect = 1.54e-312
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0

Query: 3   MKLISRFLVVLLLIVLRSEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQ 62
           MKLISRFLVVLLLIVLRSEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQ
Sbjct: 1   MKLISRFLVVLLLIVLRSEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQ 60

Query: 63  RTKVSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIK 122
           RTKVSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIK
Sbjct: 61  RTKVSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIK 120

Query: 123 LILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLT 182
           LILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLT
Sbjct: 121 LILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLT 180

Query: 183 GIAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQ 242
           GIAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQ
Sbjct: 181 GIAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQ 240

Query: 243 SRNQGNPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDA 302
           SRNQGNPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDA
Sbjct: 241 SRNQGNPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDA 300

Query: 303 QNILHKPVLFAEFGKSTKYSGSDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMD 362
           QNILHKPVLFAEFGKSTKYSGSDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMD
Sbjct: 301 QNILHKPVLFAEFGKSTKYSGSDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMD 360

Query: 363 SFRDGYEVVLNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAKEIRRAQWQALNGG 422
           SFRDGYEVVLNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAKEIRRAQWQALNGG
Sbjct: 361 SFRDGYEVVLNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAKEIRRAQWQALNGG 420

Query: 423 NNSPGN 428
           NNSPGN
Sbjct: 421 NNSPGN 426

BLAST of MC11g0154 vs. NCBI nr
Match: XP_038897716.1 (mannan endo-1,4-beta-mannosidase 7-like [Benincasa hispida])

HSP 1 Score: 764 bits (1973), Expect = 4.62e-277
Identity = 380/426 (89.20%), Postives = 405/426 (95.07%), Query Frame = 0

Query: 3   MKLISRFLVVLLLIVLRSEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQ 62
           MKL    LV+LL +VL   AEA+DGFVRTRGQQLILNG+PFYANGFNAYWLMYFASDPSQ
Sbjct: 1   MKLSISILVLLLPLVLL--AEADDGFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQ 60

Query: 63  RTKVSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIK 122
           RTKVSSAFQ+A+NHGLSIGRTWAFSDGGY+PLQYSPGQYNE+MFQGLD+V+SEA K+GIK
Sbjct: 61  RTKVSSAFQQAVNHGLSIGRTWAFSDGGYSPLQYSPGQYNEKMFQGLDFVVSEARKYGIK 120

Query: 123 LILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLT 182
           LILSLVNNYE+MGGKKQYVEWARSQGQ+ISSEDDFF+N VVKGFYKNHIKSVL R NS+T
Sbjct: 121 LILSLVNNYESMGGKKQYVEWARSQGQSISSEDDFFSNPVVKGFYKNHIKSVLTRINSIT 180

Query: 183 GIAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQ 242
           G+AYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQ
Sbjct: 181 GVAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQ 240

Query: 243 SRNQGNPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDA 302
           SR Q N NFQVGT+FIANNQIPELDFATVHSYPDQWLSGSSYENQL+FLNTWLNDHIQDA
Sbjct: 241 SRYQWNTNFQVGTDFIANNQIPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDA 300

Query: 303 QNILHKPVLFAEFGKSTKYSGSDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMD 362
           QNILHKPVL AEFGKSTKYSG+DQRDQLFNAVYSAVYSSAR GGAAVGGLFWQLLVEGMD
Sbjct: 301 QNILHKPVLLAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAVGGLFWQLLVEGMD 360

Query: 363 SFRDGYEVVLNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAKEIRRAQWQALNGG 422
           SFRDGYEVVL+EN STANLISQES++LIHIRKMYAKLRNIEK KRA+EIR+AQW+AL GG
Sbjct: 361 SFRDGYEVVLSENPSTANLISQESRRLIHIRKMYAKLRNIEKWKRAREIRKAQWEALKGG 420

Query: 423 NNSPGN 428
            NSPGN
Sbjct: 421 KNSPGN 424

BLAST of MC11g0154 vs. NCBI nr
Match: XP_004147857.3 (mannan endo-1,4-beta-mannosidase 7 [Cucumis sativus] >KGN60129.2 hypothetical protein Csa_001474 [Cucumis sativus])

HSP 1 Score: 745 bits (1924), Expect = 1.40e-269
Identity = 359/417 (86.09%), Postives = 397/417 (95.20%), Query Frame = 0

Query: 12  VLLLIVLRSEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQRTKVSSAFQ 71
           +LLL++L   A+ ++GFV T+GQQLILNGSPFYANGFNAYWLMYFASDPSQ  KVSSAFQ
Sbjct: 9   ILLLLLLLPLAKPDEGFVSTKGQQLILNGSPFYANGFNAYWLMYFASDPSQAPKVSSAFQ 68

Query: 72  EALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIKLILSLVNNY 131
           +A+NHGLSIGRTWAF+DGGY+PLQYSPGQYNE+MFQGLD+V++EA K+GIKLILSLVNNY
Sbjct: 69  QAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQGLDFVVAEARKYGIKLILSLVNNY 128

Query: 132 ENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLTGIAYKDDPT 191
            +MGGKKQYVEWARSQGQAISSED+FFTN VVKGFYKNHIKS+L R NS+TG+AYKDDPT
Sbjct: 129 ASMGGKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITGVAYKDDPT 188

Query: 192 IMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQSRNQGNPNF 251
           IMAWELMNEARCPSDPSGNTIQ WI+EMASYLKSIDGKHLLEAGLEGFYGQS+ Q NPNF
Sbjct: 189 IMAWELMNEARCPSDPSGNTIQGWIKEMASYLKSIDGKHLLEAGLEGFYGQSKYQWNPNF 248

Query: 252 QVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDAQNILHKPVL 311
           QVGT+FIANNQIPE+DFATVHSYPDQWLSGSSYENQL+FLNTWLNDHIQDAQNILHKPVL
Sbjct: 249 QVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVL 308

Query: 312 FAEFGKSTKYSGSDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMDSFRDGYEVV 371
           FAEFGKSTKYSG+DQRDQLFNAVYSAVYSSAR GGAA+GG+FWQLLVEGMDSFRDGYEVV
Sbjct: 309 FAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDSFRDGYEVV 368

Query: 372 LNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAKEIRRAQWQALNGGNNSPGN 428
           L+E+ STANLI+QES++LIHIR+MYAKLRNIEK KRAKE++RAQW+A  GGNNSPGN
Sbjct: 369 LSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKRAQWEASRGGNNSPGN 425

BLAST of MC11g0154 vs. NCBI nr
Match: XP_008466539.2 (PREDICTED: mannan endo-1,4-beta-mannosidase 7-like [Cucumis melo] >ADN34185.1 endo-beta-mannanase [Cucumis melo subsp. melo])

HSP 1 Score: 744 bits (1920), Expect = 5.69e-269
Identity = 361/423 (85.34%), Postives = 403/423 (95.27%), Query Frame = 0

Query: 6   ISRFLVVLLLIVLRSEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQRTK 65
           IS F+++ L +VL   A+A+DGFVRT+GQQLILNG+PFYANGFNAYWLMYFASDPS + K
Sbjct: 5   ISIFILLFLPLVLL--AKADDGFVRTKGQQLILNGTPFYANGFNAYWLMYFASDPSLKPK 64

Query: 66  VSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIKLIL 125
           VSSAFQ+A++HGLSIGRTWAF+DGGY+PLQYSPGQYNE+MFQGLD+V++EA K+GIKLIL
Sbjct: 65  VSSAFQQAVDHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQGLDFVVAEARKYGIKLIL 124

Query: 126 SLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLTGIA 185
           SLVNNYE+MGGKKQYVEWARSQGQAISSED+FFTN VVKGFYKNHIKS+L R NS+TG+A
Sbjct: 125 SLVNNYESMGGKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITGVA 184

Query: 186 YKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQSRN 245
           YKDDPTIMAWELMNEARCPSDPSGNTIQAWI+EMASYLKSIDGKHLLEAGLEGFYGQS+ 
Sbjct: 185 YKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQSKY 244

Query: 246 QGNPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDAQNI 305
           Q NPNFQVGT+FIANNQIPE+DFATVHSYPDQWLSGSSYENQL+FLNTWLNDHIQDAQNI
Sbjct: 245 QWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNI 304

Query: 306 LHKPVLFAEFGKSTKYSGSDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMDSFR 365
           LHKPVLFAEFGKS KYSG+DQRDQLFNAVYSAVYSSAR GGAA+GG+FWQLLVEGMDSFR
Sbjct: 305 LHKPVLFAEFGKSIKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDSFR 364

Query: 366 DGYEVVLNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAKEIRRAQWQALNGGNNS 425
           DGYEVVL+E+ STANLI+QES++LIHIR+MYAKLRNIEK KRAKE+++AQW+A  GGNNS
Sbjct: 365 DGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEASRGGNNS 424

Query: 426 PGN 428
           PGN
Sbjct: 425 PGN 425

BLAST of MC11g0154 vs. NCBI nr
Match: XP_022941053.1 (mannan endo-1,4-beta-mannosidase 7-like [Cucurbita moschata])

HSP 1 Score: 735 bits (1897), Expect = 2.03e-265
Identity = 363/428 (84.81%), Postives = 398/428 (92.99%), Query Frame = 0

Query: 3   MKLISRFLVVLLLIVLRSEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQ 62
           M+L S  LVVL+L+++   A+A+DGFV TRGQQLILNGSPFYANGFNAYW+MYF SDPSQ
Sbjct: 1   MRLWSPVLVVLVLVLVLVRAKADDGFVSTRGQQLILNGSPFYANGFNAYWVMYFGSDPSQ 60

Query: 63  RTKVSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIK 122
           R KVSSAF+EA+ HGLSIGRTWAFSDGG +PLQYSPG YNE+MFQGLD+ +SEA K+GIK
Sbjct: 61  RNKVSSAFEEAVKHGLSIGRTWAFSDGGDSPLQYSPGSYNEKMFQGLDFAVSEARKYGIK 120

Query: 123 LILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLT 182
           LILSLVNNYENMGGKKQYVEWARSQGQ+ISS+DDFF+N VVKG YKNHIKS+L R NS T
Sbjct: 121 LILSLVNNYENMGGKKQYVEWARSQGQSISSDDDFFSNPVVKGLYKNHIKSILTRINSFT 180

Query: 183 GIAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQ 242
           G+AYKDDPTIMAWELMNE RCPSDPSGNTIQAWIREM SYLKSIDGKHLLEAGLEGFYGQ
Sbjct: 181 GVAYKDDPTIMAWELMNEPRCPSDPSGNTIQAWIREMGSYLKSIDGKHLLEAGLEGFYGQ 240

Query: 243 SRNQGNPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDA 302
           SRNQGNPNFQVGT+FIANNQIPE+DFATVHSYPDQWL+GS+ +NQL+FLNTWLN+HIQDA
Sbjct: 241 SRNQGNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLTGSTNQNQLSFLNTWLNEHIQDA 300

Query: 303 QNILHKPVLFAEFGKSTKYSGSDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMD 362
           QNILHKPVLFAEFGKSTK S SDQRDQL+NAVYSAVYSSAR GGAAVGGLFWQLLVEGMD
Sbjct: 301 QNILHKPVLFAEFGKSTKNSASDQRDQLYNAVYSAVYSSARGGGAAVGGLFWQLLVEGMD 360

Query: 363 SFRDGYEVVLNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAKEIRRAQW-QALNG 422
           SFRDGYEV+L+ENLSTANLISQES++LI IRKMYA+LRNIEKLKRAKEI RA+W +AL G
Sbjct: 361 SFRDGYEVILSENLSTANLISQESRRLIRIRKMYARLRNIEKLKRAKEIGRAEWKEALKG 420

Query: 423 GNN-SPGN 428
           GNN SPGN
Sbjct: 421 GNNNSPGN 428

BLAST of MC11g0154 vs. ExPASy TrEMBL
Match: A0A6J1BS42 (Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111005053 PE=3 SV=1)

HSP 1 Score: 854 bits (2206), Expect = 7.47e-313
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0

Query: 3   MKLISRFLVVLLLIVLRSEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQ 62
           MKLISRFLVVLLLIVLRSEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQ
Sbjct: 1   MKLISRFLVVLLLIVLRSEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQ 60

Query: 63  RTKVSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIK 122
           RTKVSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIK
Sbjct: 61  RTKVSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIK 120

Query: 123 LILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLT 182
           LILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLT
Sbjct: 121 LILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLT 180

Query: 183 GIAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQ 242
           GIAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQ
Sbjct: 181 GIAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQ 240

Query: 243 SRNQGNPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDA 302
           SRNQGNPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDA
Sbjct: 241 SRNQGNPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDA 300

Query: 303 QNILHKPVLFAEFGKSTKYSGSDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMD 362
           QNILHKPVLFAEFGKSTKYSGSDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMD
Sbjct: 301 QNILHKPVLFAEFGKSTKYSGSDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMD 360

Query: 363 SFRDGYEVVLNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAKEIRRAQWQALNGG 422
           SFRDGYEVVLNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAKEIRRAQWQALNGG
Sbjct: 361 SFRDGYEVVLNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAKEIRRAQWQALNGG 420

Query: 423 NNSPGN 428
           NNSPGN
Sbjct: 421 NNSPGN 426

BLAST of MC11g0154 vs. ExPASy TrEMBL
Match: A0A0A0LDZ5 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_3G859700 PE=3 SV=1)

HSP 1 Score: 746 bits (1927), Expect = 2.36e-270
Identity = 359/417 (86.09%), Postives = 397/417 (95.20%), Query Frame = 0

Query: 12  VLLLIVLRSEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQRTKVSSAFQ 71
           +LLL++L   A+ ++GFV T+GQQLILNGSPFYANGFNAYWLMYFASDPSQ  KVSSAFQ
Sbjct: 9   ILLLLLLLPRAKPDEGFVSTKGQQLILNGSPFYANGFNAYWLMYFASDPSQAPKVSSAFQ 68

Query: 72  EALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIKLILSLVNNY 131
           +A+NHGLSIGRTWAF+DGGY+PLQYSPGQYNE+MFQGLD+V++EA K+GIKLILSLVNNY
Sbjct: 69  QAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQGLDFVVAEARKYGIKLILSLVNNY 128

Query: 132 ENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLTGIAYKDDPT 191
            +MGGKKQYVEWARSQGQAISSED+FFTN VVKGFYKNHIKS+L R NS+TG+AYKDDPT
Sbjct: 129 ASMGGKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITGVAYKDDPT 188

Query: 192 IMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQSRNQGNPNF 251
           IMAWELMNEARCPSDPSGNTIQ WI+EMASYLKSIDGKHLLEAGLEGFYGQS+ Q NPNF
Sbjct: 189 IMAWELMNEARCPSDPSGNTIQGWIKEMASYLKSIDGKHLLEAGLEGFYGQSKYQWNPNF 248

Query: 252 QVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDAQNILHKPVL 311
           QVGT+FIANNQIPE+DFATVHSYPDQWLSGSSYENQL+FLNTWLNDHIQDAQNILHKPVL
Sbjct: 249 QVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVL 308

Query: 312 FAEFGKSTKYSGSDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMDSFRDGYEVV 371
           FAEFGKSTKYSG+DQRDQLFNAVYSAVYSSAR GGAA+GG+FWQLLVEGMDSFRDGYEVV
Sbjct: 309 FAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDSFRDGYEVV 368

Query: 372 LNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAKEIRRAQWQALNGGNNSPGN 428
           L+E+ STANLI+QES++LIHIR+MYAKLRNIEK KRAKE++RAQW+A  GGNNSPGN
Sbjct: 369 LSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKRAQWEASRGGNNSPGN 425

BLAST of MC11g0154 vs. ExPASy TrEMBL
Match: E5GCI6 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 744 bits (1920), Expect = 2.75e-269
Identity = 361/423 (85.34%), Postives = 403/423 (95.27%), Query Frame = 0

Query: 6   ISRFLVVLLLIVLRSEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQRTK 65
           IS F+++ L +VL   A+A+DGFVRT+GQQLILNG+PFYANGFNAYWLMYFASDPS + K
Sbjct: 5   ISIFILLFLPLVLL--AKADDGFVRTKGQQLILNGTPFYANGFNAYWLMYFASDPSLKPK 64

Query: 66  VSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIKLIL 125
           VSSAFQ+A++HGLSIGRTWAF+DGGY+PLQYSPGQYNE+MFQGLD+V++EA K+GIKLIL
Sbjct: 65  VSSAFQQAVDHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQGLDFVVAEARKYGIKLIL 124

Query: 126 SLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLTGIA 185
           SLVNNYE+MGGKKQYVEWARSQGQAISSED+FFTN VVKGFYKNHIKS+L R NS+TG+A
Sbjct: 125 SLVNNYESMGGKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITGVA 184

Query: 186 YKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQSRN 245
           YKDDPTIMAWELMNEARCPSDPSGNTIQAWI+EMASYLKSIDGKHLLEAGLEGFYGQS+ 
Sbjct: 185 YKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQSKY 244

Query: 246 QGNPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDAQNI 305
           Q NPNFQVGT+FIANNQIPE+DFATVHSYPDQWLSGSSYENQL+FLNTWLNDHIQDAQNI
Sbjct: 245 QWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNI 304

Query: 306 LHKPVLFAEFGKSTKYSGSDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMDSFR 365
           LHKPVLFAEFGKS KYSG+DQRDQLFNAVYSAVYSSAR GGAA+GG+FWQLLVEGMDSFR
Sbjct: 305 LHKPVLFAEFGKSIKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDSFR 364

Query: 366 DGYEVVLNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAKEIRRAQWQALNGGNNS 425
           DGYEVVL+E+ STANLI+QES++LIHIR+MYAKLRNIEK KRAKE+++AQW+A  GGNNS
Sbjct: 365 DGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEASRGGNNS 424

Query: 426 PGN 428
           PGN
Sbjct: 425 PGN 425

BLAST of MC11g0154 vs. ExPASy TrEMBL
Match: A0A1S3CRH7 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103503926 PE=3 SV=1)

HSP 1 Score: 744 bits (1920), Expect = 2.75e-269
Identity = 361/423 (85.34%), Postives = 403/423 (95.27%), Query Frame = 0

Query: 6   ISRFLVVLLLIVLRSEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQRTK 65
           IS F+++ L +VL   A+A+DGFVRT+GQQLILNG+PFYANGFNAYWLMYFASDPS + K
Sbjct: 5   ISIFILLFLPLVLL--AKADDGFVRTKGQQLILNGTPFYANGFNAYWLMYFASDPSLKPK 64

Query: 66  VSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIKLIL 125
           VSSAFQ+A++HGLSIGRTWAF+DGGY+PLQYSPGQYNE+MFQGLD+V++EA K+GIKLIL
Sbjct: 65  VSSAFQQAVDHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQGLDFVVAEARKYGIKLIL 124

Query: 126 SLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLTGIA 185
           SLVNNYE+MGGKKQYVEWARSQGQAISSED+FFTN VVKGFYKNHIKS+L R NS+TG+A
Sbjct: 125 SLVNNYESMGGKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITGVA 184

Query: 186 YKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQSRN 245
           YKDDPTIMAWELMNEARCPSDPSGNTIQAWI+EMASYLKSIDGKHLLEAGLEGFYGQS+ 
Sbjct: 185 YKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQSKY 244

Query: 246 QGNPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDAQNI 305
           Q NPNFQVGT+FIANNQIPE+DFATVHSYPDQWLSGSSYENQL+FLNTWLNDHIQDAQNI
Sbjct: 245 QWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNI 304

Query: 306 LHKPVLFAEFGKSTKYSGSDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMDSFR 365
           LHKPVLFAEFGKS KYSG+DQRDQLFNAVYSAVYSSAR GGAA+GG+FWQLLVEGMDSFR
Sbjct: 305 LHKPVLFAEFGKSIKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDSFR 364

Query: 366 DGYEVVLNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAKEIRRAQWQALNGGNNS 425
           DGYEVVL+E+ STANLI+QES++LIHIR+MYAKLRNIEK KRAKE+++AQW+A  GGNNS
Sbjct: 365 DGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEASRGGNNS 424

Query: 426 PGN 428
           PGN
Sbjct: 425 PGN 425

BLAST of MC11g0154 vs. ExPASy TrEMBL
Match: A0A6J1FR05 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111446456 PE=3 SV=1)

HSP 1 Score: 735 bits (1897), Expect = 9.82e-266
Identity = 363/428 (84.81%), Postives = 398/428 (92.99%), Query Frame = 0

Query: 3   MKLISRFLVVLLLIVLRSEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQ 62
           M+L S  LVVL+L+++   A+A+DGFV TRGQQLILNGSPFYANGFNAYW+MYF SDPSQ
Sbjct: 1   MRLWSPVLVVLVLVLVLVRAKADDGFVSTRGQQLILNGSPFYANGFNAYWVMYFGSDPSQ 60

Query: 63  RTKVSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIK 122
           R KVSSAF+EA+ HGLSIGRTWAFSDGG +PLQYSPG YNE+MFQGLD+ +SEA K+GIK
Sbjct: 61  RNKVSSAFEEAVKHGLSIGRTWAFSDGGDSPLQYSPGSYNEKMFQGLDFAVSEARKYGIK 120

Query: 123 LILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLT 182
           LILSLVNNYENMGGKKQYVEWARSQGQ+ISS+DDFF+N VVKG YKNHIKS+L R NS T
Sbjct: 121 LILSLVNNYENMGGKKQYVEWARSQGQSISSDDDFFSNPVVKGLYKNHIKSILTRINSFT 180

Query: 183 GIAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQ 242
           G+AYKDDPTIMAWELMNE RCPSDPSGNTIQAWIREM SYLKSIDGKHLLEAGLEGFYGQ
Sbjct: 181 GVAYKDDPTIMAWELMNEPRCPSDPSGNTIQAWIREMGSYLKSIDGKHLLEAGLEGFYGQ 240

Query: 243 SRNQGNPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDA 302
           SRNQGNPNFQVGT+FIANNQIPE+DFATVHSYPDQWL+GS+ +NQL+FLNTWLN+HIQDA
Sbjct: 241 SRNQGNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLTGSTNQNQLSFLNTWLNEHIQDA 300

Query: 303 QNILHKPVLFAEFGKSTKYSGSDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMD 362
           QNILHKPVLFAEFGKSTK S SDQRDQL+NAVYSAVYSSAR GGAAVGGLFWQLLVEGMD
Sbjct: 301 QNILHKPVLFAEFGKSTKNSASDQRDQLYNAVYSAVYSSARGGGAAVGGLFWQLLVEGMD 360

Query: 363 SFRDGYEVVLNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAKEIRRAQW-QALNG 422
           SFRDGYEV+L+ENLSTANLISQES++LI IRKMYA+LRNIEKLKRAKEI RA+W +AL G
Sbjct: 361 SFRDGYEVILSENLSTANLISQESRRLIRIRKMYARLRNIEKLKRAKEIGRAEWKEALKG 420

Query: 423 GNN-SPGN 428
           GNN SPGN
Sbjct: 421 GNNNSPGN 428

BLAST of MC11g0154 vs. TAIR 10
Match: AT5G66460.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 553.1 bits (1424), Expect = 1.9e-157
Identity = 266/417 (63.79%), Postives = 332/417 (79.62%), Query Frame = 0

Query: 3   MKLISRFLVVLLLIVLR-----SEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFA 62
           MKL++ F  + ++I L      ++A    GFVRT+G Q  LNG P+YANGFNAYWLMY A
Sbjct: 1   MKLLALFPFLAIVIQLSCWELGTDALPSGGFVRTKGVQFSLNGYPYYANGFNAYWLMYVA 60

Query: 63  SDPSQRTKVSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAG 122
           SDPSQR+K+S+AFQ+A  HGL++ RTWAFSDGGY  LQYSPG YNE MFQGLD+ ++EA 
Sbjct: 61  SDPSQRSKISTAFQDASRHGLTVARTWAFSDGGYRALQYSPGSYNEDMFQGLDFALAEAR 120

Query: 123 KHGIKLILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMR 182
           +HGIK+ILS  NNYE+ GG+KQYV+WARS+G+ +SSEDDFFT+S+VK FYKNHIK+VL R
Sbjct: 121 RHGIKIILSFANNYESFGGRKQYVDWARSRGRPVSSEDDFFTDSLVKDFYKNHIKAVLNR 180

Query: 183 TNSLTGIAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLE 242
            N+ T + YKDDPTIMAWELMNE RCPSDPSG  IQAWI EMA+++KS+D  HLLEAGLE
Sbjct: 181 FNTFTKVHYKDDPTIMAWELMNEPRCPSDPSGRAIQAWITEMAAHVKSLDRNHLLEAGLE 240

Query: 243 GFYGQSRNQG---NPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTW 302
           GFYGQS  Q    NP  Q GT+FIANN+IP +DF TVHSYPD+W   SS ++Q+ FLN W
Sbjct: 241 GFYGQSSPQSKTLNPPGQFGTDFIANNRIPGIDFVTVHSYPDEWFPDSSEQSQMDFLNKW 300

Query: 303 LNDHIQDAQNILHKPVLFAEFGKSTKYSG--SDQRDQLFNAVYSAVYSSARTGGAAVGGL 362
           L+ HIQDAQN+LHKP++ AEFGKS K  G    QRD +FN VYS +Y SA+ GGAA GGL
Sbjct: 301 LDAHIQDAQNVLHKPIILAEFGKSMKKPGYTPAQRDIVFNTVYSKIYGSAKRGGAAAGGL 360

Query: 363 FWQLLVEGMDSFRDGYEVVLNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAK 410
           FWQLLV G+D+F+DGY ++L+++ ST N+ISQ+S+KL  IRK++A++ N+EK KRA+
Sbjct: 361 FWQLLVNGIDNFQDGYGIILSQSSSTVNVISQQSRKLTLIRKIFARMINVEKWKRAR 417

BLAST of MC11g0154 vs. TAIR 10
Match: AT3G10890.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 431.4 bits (1108), Expect = 8.4e-121
Identity = 201/399 (50.38%), Postives = 281/399 (70.43%), Query Frame = 0

Query: 9   FLVVLLLIVLRSEAE------AEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQ 68
           F+V+L +++ ++ ++      + DGFV  +G Q ILNG PFYANGFNAYWL Y A+D + 
Sbjct: 6   FVVLLAILIAQNSSDLGVKSASSDGFVSRKGVQFILNGKPFYANGFNAYWLAYEATDSTT 65

Query: 69  RTKVSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIK 128
           R K++  FQ A  H L+I RTW F DGGY  LQ +PG Y+E+ FQGLD+ I+EA + GIK
Sbjct: 66  RFKITYVFQNATIHDLTIVRTWGFRDGGYRALQIAPGVYDEKTFQGLDFAIAEAKRLGIK 125

Query: 129 LILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLT 188
           +I++ VNNY + GG+KQYV+WA++ GQ +SS+DDF+TN +VK +YKNH+K+++ R N+ T
Sbjct: 126 MIITFVNNYSDFGGRKQYVDWAKNTGQNVSSDDDFYTNPLVKQYYKNHVKTMVNRVNTFT 185

Query: 189 GIAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQ 248
            + YKD+PTIM WELMNE +C +DPSG T+ AW+ EMA Y+KS+D KHLL  GLEGFYG 
Sbjct: 186 KVEYKDEPTIMGWELMNEPQCRADPSGKTLTAWMNEMALYVKSVDSKHLLSTGLEGFYGD 245

Query: 249 SRNQGNPNFQ------VGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLN 308
           S  Q   +        +GT+FIAN+++  +DFA++HSYPD W      +++L  L  WL 
Sbjct: 246 SSPQRKTSLNPVAANVLGTDFIANHKLDAIDFASIHSYPDLWFPNLDEKSRLNLLRKWLE 305

Query: 309 DHIQDAQNILHKPVLFAEFGKSTKYSG--SDQRDQLFNAVYSAVYSSARTGGAAVGGLFW 368
            H++DAQNIL KP++  EFGK T   G    QRD +FNA +  +Y SA  GG A G LFW
Sbjct: 306 CHLEDAQNILKKPLILGEFGKPTNTPGYTQAQRDAVFNATFDTIYESAEKGGPAAGALFW 365

Query: 369 QLLVEGMDSFRDGYEVVLNENLSTANLISQESQKLIHIR 394
            ++ +GM++F+D   +VL+EN +T N+I++ES+KL  IR
Sbjct: 366 HVISDGMNNFKDPLSIVLSENSTTVNIITEESRKLGLIR 404

BLAST of MC11g0154 vs. TAIR 10
Match: AT3G10900.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 409.1 bits (1050), Expect = 4.5e-114
Identity = 201/394 (51.02%), Postives = 268/394 (68.02%), Query Frame = 0

Query: 9   FLVVLLLIVLRS----EAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQRT 68
           F+V+L +++ +S    EA   DGFV   G Q ILNG PFYANGFNAYWL Y A+DP+ R 
Sbjct: 6   FIVLLAIVIAQSYVGVEAAPSDGFVSRNGVQFILNGKPFYANGFNAYWLAYEATDPTTRF 65

Query: 69  KVSSAFQEALNHGLSIGRTWAFSDGG-YNPLQYSPGQYNEQMFQGLDYVISEAGKHGIKL 128
           K+++ FQ A + GL+I RTW F DG  Y  LQ +PG Y+EQ FQGLD+VI+EA + GIKL
Sbjct: 66  KITNVFQNATSLGLTIARTWGFRDGAIYRALQTAPGSYDEQTFQGLDFVIAEAKRIGIKL 125

Query: 129 ILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLTG 188
           I+ LVNN+++ GGKKQYV+WARS+G+ +SS DDF+ N V+K FYKNH+K+VL R N+ T 
Sbjct: 126 IILLVNNWDDYGGKKQYVDWARSKGEVVSSNDDFYRNPVIKDFYKNHVKTVLNRVNTFTK 185

Query: 189 IAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQS 248
           +AYKD+P IMAW+LMNE RC  D SG T+  WI EMA ++KS+D  HLL  G EGFYG S
Sbjct: 186 VAYKDEPAIMAWQLMNEPRCGVDKSGKTLMDWINEMAPFVKSVDPNHLLSTGHEGFYGDS 245

Query: 249 ----RNQGNP--NFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLND 308
               +N  NP     VG +FIAN+ I  +DFA++H   D W       ++L F+  WL  
Sbjct: 246 SPERKNSLNPVSANTVGADFIANHNIDAIDFASMHCGSDLWFQRLDQNSRLAFIKRWLEG 305

Query: 309 HIQDAQNILHKPVLFAEF--GKSTKYSGSDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQ 368
           HI+DAQNIL KPV+ AEF  G  T       RD +F   Y  +Y+SA+ GG+A G LFW+
Sbjct: 306 HIEDAQNILKKPVILAEFGLGSDTPRYTLANRDGVFTTTYDIIYASAQKGGSAAGALFWE 365

Query: 369 LLVEGMDSFRDGYEVVLNENLSTANLISQESQKL 390
           ++ EGM +F     ++L++  ST N+IS+ ++K+
Sbjct: 366 VISEGMSNFAGPSSIILSDKSSTVNIISEHARKM 399

BLAST of MC11g0154 vs. TAIR 10
Match: AT5G01930.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 391.7 bits (1005), Expect = 7.4e-109
Identity = 188/378 (49.74%), Postives = 258/378 (68.25%), Query Frame = 0

Query: 20  SEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQRTKVSSAFQEALNHGLS 79
           S  E +   V+ +G Q  LNG PFY NGFN YW+M  A+D S R KV+  FQ+A   G++
Sbjct: 39  SVGEEQWEMVQRKGMQFTLNGQPFYVNGFNTYWMMTLAADNSTRGKVTEVFQQASAVGMT 98

Query: 80  IGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIKLILSLVNNYENMGGKKQ 139
           +GRTWAF+DG +  LQ SP  Y+E++F+ LD+V+SEA K+ I+LILSLVNN++  GGK Q
Sbjct: 99  VGRTWAFNDGQWRALQKSPSVYDEEVFKALDFVLSEARKYKIRLILSLVNNWDAYGGKAQ 158

Query: 140 YVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLTGIAYKDDPTIMAWELMN 199
           YV+W  + G  ++S+DDFFTN  ++ FY++H+++VL R N+ T I YK+DPTI AWELMN
Sbjct: 159 YVKWGNASGLNLTSDDDFFTNPTLRNFYQSHVRTVLNRVNTFTNITYKNDPTIFAWELMN 218

Query: 200 EARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQS---RNQGNPN---FQV 259
           E RCPSDPSG+ +Q+WI+EMA ++KS+D KHL+E GLEGFYG S   R + NPN    QV
Sbjct: 219 EPRCPSDPSGDKLQSWIQEMAVFVKSLDAKHLVEIGLEGFYGPSAPARTRFNPNPYAAQV 278

Query: 260 GTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDAQNILHKPVLFA 319
           GT+FI NNQ+  +DFA+VH YPD W+S +   + L F ++W+  H++DA+  L  PVLF 
Sbjct: 279 GTDFIRNNQVLGIDFASVHVYPDSWISPAVSNSFLEFTSSWMQAHVEDAEMYLGMPVLFT 338

Query: 320 EFGKSTKYSG--SDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMDSFRDGYEVV 379
           EFG S    G  +  RD + N VY    +S R GGA  G L WQ+  +G +   DGY V 
Sbjct: 339 EFGVSAHDPGFNTSFRDMMLNTVYKMTLNSTRKGGAGAGSLVWQVFPQGAEFMDDGYAVY 398

Query: 380 LNENLSTANLISQESQKL 390
           L    + + +IS +S++L
Sbjct: 399 LTRAHTASKIISLQSKRL 416

BLAST of MC11g0154 vs. TAIR 10
Match: AT1G02310.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 389.4 bits (999), Expect = 3.7e-108
Identity = 192/379 (50.66%), Postives = 257/379 (67.81%), Query Frame = 0

Query: 27  GFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQ--RTKVSSAFQEALNHGLSIGRTW 86
           GFV   G Q +LNG   Y NGFNAYW+M  A+D +   R  V++A ++A   G+++ R W
Sbjct: 28  GFVGRNGTQFVLNGEQVYLNGFNAYWMMTTAADTASKGRATVTTALRQASAVGMNVARIW 87

Query: 87  AFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIKLILSLVNNYENMGGKKQYVEWA 146
            F++G Y PLQ SPG Y+E +F+GLD+V+ EAG+  IKLI+SLVNN+E+ GG+K+YVEWA
Sbjct: 88  GFNEGDYIPLQISPGSYSEDVFKGLDFVVYEAGRFNIKLIISLVNNFEDYGGRKKYVEWA 147

Query: 147 RSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLTGIAYKDDPTIMAWELMNEARCP 206
                 +   D+F+TNS VK FYKNH+K+VL R N++TG  YKDDPTI +WEL+NE RC 
Sbjct: 148 -----GLDEPDEFYTNSAVKQFYKNHVKTVLTRKNTITGRMYKDDPTIFSWELINEPRCN 207

Query: 207 SDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQS---RNQGNPNFQV--GTNFIA 266
              + N +Q W++EMASY+KSID  HLLE GLEGFYG+S   R   NP  +V  GT+FI 
Sbjct: 208 DSTASNILQDWVKEMASYVKSIDSNHLLEIGLEGFYGESIPERTVYNPGGRVLTGTDFIT 267

Query: 267 NNQIPELDFATVHSYPDQW--LSGSSYENQLTFLNTWLNDHIQDAQNILHKPVLFAEFGK 326
           NNQIP++DFAT+H YPD W  L  S    Q TF++ W+  HI+D  NI+ KP+L  EFGK
Sbjct: 268 NNQIPDIDFATIHIYPDSWLPLQSSRTGEQDTFVDRWIGAHIEDCDNIIKKPLLITEFGK 327

Query: 327 STKYSG--SDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMDSFRDGYEVVLNEN 386
           S+KY G   ++R++ F  VY  +Y SAR GG+  GG+FWQL         DGYEV +   
Sbjct: 328 SSKYPGFSLEKRNKFFQRVYDVIYDSARAGGSCTGGVFWQLTTNRTGLLGDGYEVFMQAG 387

Query: 387 -LSTANLISQESQKLIHIR 394
             +TA LI+ +S KL +++
Sbjct: 388 PNTTAQLIADQSSKLKNLK 401