MC11g0090 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g0090 vs. ExPASy Swiss-Prot
Match: Q9ASS2 (Protein FREE1 OS=Arabidopsis thaliana OX=3702 GN=FREE1 PE=1 SV=1)

HSP 1 Score: 684.1 bits (1764), Expect = 1.4e-195
Identity = 383/623 (61.48%), Postives = 447/623 (71.75%), Query Frame = 0

Query: 10  MQHGDYGTAPYFQYPNLQNLSLNPSPNPVPTPP---------DRNQSAYASAPPFSTNYG 69
           MQ GDY +  + QY   QN + NP+PNP P+PP         D  ++ YASAPPF+  YG
Sbjct: 1   MQQGDYNSYYHHQYSQFQNPTPNPNPNPNPSPPAPATVAGPTDLTRNTYASAPPFTGGYG 60

Query: 70  GSDYPGYASNYAHYSQNPDPVPASPTAPLYTPPSS----------NPN-LQTFNPTPQPP 129
            +DY  Y+ NY  Y QN + VP  P+AP +T PS           NPN   TFN  P PP
Sbjct: 61  SADYSNYSQNYTPYGQNSEHVP--PSAPSFTSPSQPPPSPPATSLNPNSYSTFNQPPPPP 120

Query: 130 AFPP--------FESHTPYQPPSQPQSYHSTYDQNQTAPNYAPPPPSSIPVNPSSNSTPS 189
              P        F+S  PYQ P+    Y+S YDQ+QT+   + PPPSS    P+ N  P+
Sbjct: 121 TIHPQPLSSYGSFDSTAPYQQPTSQHMYYSPYDQHQTSGYSSAPPPSSA---PAPNPNPA 180

Query: 190 PNSPFSSMYSAP----FGSLPPPAYENPYESSVKFDQGGGYVDDRYGGYSRSRSDLGPDL 249
           P S  SS+YSAP     GS  PP+YE P   SVKFDQ G      Y GY+RSRSDLG DL
Sbjct: 181 PYS--SSLYSAPPYSSGGSSIPPSYEKP---SVKFDQSG------YDGYNRSRSDLGSDL 240

Query: 250 YGKRPEDTISRFDSAYDDGFGDGVYAYQGGKVEPYGARGTASKSSTWSAAPAFDDYGRPI 309
           YGKR +   S    A++D +GDGVYAYQGGKVEPYG+RGTA KSS    +  FDDYGR I
Sbjct: 241 YGKRSD---SGEYPAFEDSYGDGVYAYQGGKVEPYGSRGTAPKSSN---STLFDDYGRSI 300

Query: 310 NIPPK-KDS--SATSLKVVRATPKADAQEDVKSWVQKFRVKLLAESGGQSTMDVLCQVGL 369
           +     +DS  S+ S K+VRA PKAD QED    VQKFRVKLLAE+ GQ+T DVLCQ+GL
Sbjct: 301 SFSSSGRDSSVSSNSAKIVRAVPKADVQEDSTGGVQKFRVKLLAETYGQTTTDVLCQIGL 360

Query: 370 DGIRMLDPNSNRTLRIYPLETITRCEVYDSSTLAFWSKSPVDIEPRRIRLQSNRYTTNTL 429
           DG+RMLDP+++RTLRIYPLE ITRCE  DSS LAFWSK+PVDIE +RIRLQSN YTTNTL
Sbjct: 361 DGLRMLDPSTSRTLRIYPLENITRCEKLDSSILAFWSKTPVDIEAKRIRLQSNSYTTNTL 420

Query: 430 LDTVTAATVQFKEMGGRSRPSDSFKAPEQPTEKKKGLVDWVNLIKPGNEEKDHWVPDEAV 489
           LDTVTAA  Q KE+GG SRP  S K  EQ  EKKKGL DW+N+IKP NEEKDHWVPDEAV
Sbjct: 421 LDTVTAAMFQAKEIGGSSRPPTSGKLIEQTAEKKKGLGDWMNIIKPVNEEKDHWVPDEAV 480

Query: 490 TKCTSCGTDFGAFVRKHHCRNCGDIFCDKCTQGRTALTAEENVPQVRVCDRCMAEVTQRL 549
           +KCTSCG+DFGAF+R+HHCRNCGD+FCDKCTQGR ALTAE+N PQVRVCDRCMAEV+QRL
Sbjct: 481 SKCTSCGSDFGAFIRRHHCRNCGDVFCDKCTQGRIALTAEDNAPQVRVCDRCMAEVSQRL 540

Query: 550 ANAKEMASKPAGLHSHEDLAKKLKDEMDKNRRSSGSKSDGFGRRMKEVACPTCTVNLQVQ 598
           +NAKE   +   L SHEDLA+KL++EM++NR+SS    +G GRRMKEVACPTCTV+LQVQ
Sbjct: 541 SNAKETTGRNVSLQSHEDLARKLQEEMERNRKSSSGLREGSGRRMKEVACPTCTVHLQVQ 600

BLAST of MC11g0090 vs. ExPASy Swiss-Prot
Match: Q54CH1 (Arrestin domain-containing protein A OS=Dictyostelium discoideum OX=44689 GN=adcA PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 6.7e-12
Identity = 36/89 (40.45%), Postives = 46/89 (51.69%), Query Frame = 0

Query: 438 IKPGNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIFCDKCTQGRTALTAEENV 497
           + P    +  W  DE  T C  C   F  F RKHHCR+C  IFCDKCT  +T +T     
Sbjct: 455 LPPDVSYRHPWEGDEHATACRKCNKGFSLFARKHHCRHCMKIFCDKCTSTKTTITKLAYP 514

Query: 498 PQVRVCDRCMAEVTQ---RLANAKEMASK 524
             VRVC+ C    TQ   +  +AK MA++
Sbjct: 515 KPVRVCEECYPIATQGGNKYQSAKLMAAQ 543

BLAST of MC11g0090 vs. ExPASy Swiss-Prot
Match: Q8IZQ1 (WD repeat and FYVE domain-containing protein 3 OS=Homo sapiens OX=9606 GN=WDFY3 PE=1 SV=2)

HSP 1 Score: 73.9 bits (180), Expect = 6.7e-12
Identity = 29/73 (39.73%), Postives = 39/73 (53.42%), Query Frame = 0

Query: 434  WVNLIKPGNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIFCDKCTQGRTALTA 493
            W    +PG    DHWV DE    C+ C   F    R+HHCRNCG +FC KC++ ++ +  
Sbjct: 3437 WSVSDQPGRSAADHWVKDEGGDSCSGCSVRFSLTERRHHCRNCGQLFCQKCSRFQSEIKR 3496

Query: 494  EENVPQVRVCDRC 507
             +    VRVC  C
Sbjct: 3497 LKISSPVRVCQNC 3509

BLAST of MC11g0090 vs. ExPASy Swiss-Prot
Match: Q6VNB8 (WD repeat and FYVE domain-containing protein 3 OS=Mus musculus OX=10090 GN=Wdfy3 PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 6.7e-12
Identity = 29/73 (39.73%), Postives = 39/73 (53.42%), Query Frame = 0

Query: 434  WVNLIKPGNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIFCDKCTQGRTALTA 493
            W    +PG    DHWV DE    C+ C   F    R+HHCRNCG +FC KC++ ++ +  
Sbjct: 3419 WSVSDQPGRSAADHWVKDEGGDSCSGCSVRFSLTERRHHCRNCGQLFCQKCSRFQSEIKR 3478

Query: 494  EENVPQVRVCDRC 507
             +    VRVC  C
Sbjct: 3479 LKISSPVRVCQNC 3491

BLAST of MC11g0090 vs. ExPASy Swiss-Prot
Match: Q6ZPK7 (Lateral signaling target protein 2 homolog OS=Mus musculus OX=10090 GN=Zfyve28 PE=1 SV=2)

HSP 1 Score: 72.0 bits (175), Expect = 2.5e-11
Identity = 31/64 (48.44%), Postives = 35/64 (54.69%), Query Frame = 0

Query: 443 EEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIFCDKCTQGRTALTAEENVPQVRV 502
           E+   WVPDEA   CTSC   F    RKHHCR+CG IFC +C+     L     V  VRV
Sbjct: 827 EDPPEWVPDEACGFCTSCKAPFTVIRRKHHCRSCGKIFCSRCSSHSAPLPRYGQVKPVRV 886

Query: 503 CDRC 507
           C  C
Sbjct: 887 CTHC 890

BLAST of MC11g0090 vs. NCBI nr
Match: XP_022136866.1 (protein FREE1 [Momordica charantia])

HSP 1 Score: 1204 bits (3116), Expect = 0.0
Identity = 594/597 (99.50%), Postives = 595/597 (99.66%), Query Frame = 0

Query: 1   MANFSSASAMQHGDYGTAPYFQYPNLQNLSLNPSPNPVPTPPDRNQSAYASAPPFSTNYG 60
           MANFSSASAMQHGDYGTAPYFQYPNLQNLSLNPSPNPVPTPPDRNQSAYASAPPFSTNYG
Sbjct: 1   MANFSSASAMQHGDYGTAPYFQYPNLQNLSLNPSPNPVPTPPDRNQSAYASAPPFSTNYG 60

Query: 61  GSDYPGYASNYAHYSQNPDPVPASPTAPLYTPPSSNPNLQTFNPTPQPPAFPPFESHTPY 120
           GSDYPGYASNYAHYSQNPDPVPASPTAPL+TPPSSNPNLQTFNPTPQPPAFPPFESH PY
Sbjct: 61  GSDYPGYASNYAHYSQNPDPVPASPTAPLFTPPSSNPNLQTFNPTPQPPAFPPFESHAPY 120

Query: 121 QPPSQPQSYHSTYDQNQTAPNYAPPPPSSIPVNPSSNSTPSPNSPFSSMYSAPFGSLPPP 180
           QPPSQPQSYHSTYDQNQTAPNYAPPPPSSIPVNPSSNSTPSPNSPFSSMYSAPFGSLPPP
Sbjct: 121 QPPSQPQSYHSTYDQNQTAPNYAPPPPSSIPVNPSSNSTPSPNSPFSSMYSAPFGSLPPP 180

Query: 181 AYENPYESSVKFDQGGGYVDDRYGGYSRSRSDLGPDLYGKRPEDTISRFDSAYDDGFGDG 240
           AYENPYESSVKFDQGGGYVDDRYGGYSRSRSDLGPDLYGKRPEDTISRFDSAYDDGFGDG
Sbjct: 181 AYENPYESSVKFDQGGGYVDDRYGGYSRSRSDLGPDLYGKRPEDTISRFDSAYDDGFGDG 240

Query: 241 VYAYQGGKVEPYGARGTASKSSTWSAAPAFDDYGRPINIPPKKDSSATSLKVVRATPKAD 300
           VYAYQGGKVEPYGARGTASKSSTWSAAPAFDDYGRPINIPPKKDSSATSLKVVRATPKAD
Sbjct: 241 VYAYQGGKVEPYGARGTASKSSTWSAAPAFDDYGRPINIPPKKDSSATSLKVVRATPKAD 300

Query: 301 AQEDVKSWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSNRTLRIYPLETITRCE 360
           AQEDVKSWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSNRTLRIYPLETITRCE
Sbjct: 301 AQEDVKSWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSNRTLRIYPLETITRCE 360

Query: 361 VYDSSTLAFWSKSPVDIEPRRIRLQSNRYTTNTLLDTVTAATVQFKEMGGRSRPSDSFKA 420
           VYDSSTLAFWSKSPVDIEPRRIRLQSNRYTTNTLLDTVTAATVQFKEMGGRSRPSDSFKA
Sbjct: 361 VYDSSTLAFWSKSPVDIEPRRIRLQSNRYTTNTLLDTVTAATVQFKEMGGRSRPSDSFKA 420

Query: 421 PEQPTEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIF 480
           PEQPTEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIF
Sbjct: 421 PEQPTEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIF 480

Query: 481 CDKCTQGRTALTAEENVPQVRVCDRCMAEVTQRLANAKEMASKPAGLHSHEDLAKKLKDE 540
           CDKCTQGRTALTAEENVPQVRVCDRCMAEVTQRLANAKEMASKPAGLHSHEDLAKKLKDE
Sbjct: 481 CDKCTQGRTALTAEENVPQVRVCDRCMAEVTQRLANAKEMASKPAGLHSHEDLAKKLKDE 540

Query: 541 MDKNRRSSGSKSDGFGRRMKEVACPTCTVNLQVQVPSSGSETIECGVCQHPFLVSAH 597
           MDKNRRSSGSKSDGFG RMKEVACPTCTVNLQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 MDKNRRSSGSKSDGFGSRMKEVACPTCTVNLQVQVPSSGSETIECGVCQHPFLVSAH 597

BLAST of MC11g0090 vs. NCBI nr
Match: XP_038898578.1 (protein FREE1 [Benincasa hispida])

HSP 1 Score: 1071 bits (2771), Expect = 0.0
Identity = 530/591 (89.68%), Postives = 555/591 (93.91%), Query Frame = 0

Query: 10  MQHGDYGTAPYFQYPNLQNLSLNPSPNPVPTPPDRNQSAYASAPPFSTNYGGSDYPGYAS 69
           MQ+GDYGTAPYFQYPNLQNL+LNPSPNPVPTP DRNQSAYASAPPFSTNYG SDYP YA 
Sbjct: 1   MQNGDYGTAPYFQYPNLQNLTLNPSPNPVPTPSDRNQSAYASAPPFSTNYGVSDYPSYAP 60

Query: 70  NYAHYSQNPDPVPASPTAPLYTPPSSNPNLQTFNPTPQPPAFPPFESHTPYQPPSQPQSY 129
           NY  YSQNPDPVPASPTAPLY PPSSNPNLQTFNPTPQPPAFPPFESH PYQ PSQ QSY
Sbjct: 61  NYPPYSQNPDPVPASPTAPLYNPPSSNPNLQTFNPTPQPPAFPPFESHVPYQSPSQSQSY 120

Query: 130 HSTYDQNQTAPNYAPPPP--SSIPVNPSSNSTPSPNSPFSSMYSAPFGSLPPPAYENPYE 189
           HSTYDQ+QTA NY PPPP  SSIP+N SSNSTP+PNSPFSSMYSAPFGSL PP+YENPYE
Sbjct: 121 HSTYDQHQTASNYGPPPPPPSSIPMNQSSNSTPTPNSPFSSMYSAPFGSLAPPSYENPYE 180

Query: 190 SSVKFDQGGGYVDDRYGGYSRSRSDLGPDLYGKRPEDTISRFDSAYDDGFGDGVYAYQGG 249
           SSVKF+QGGG+ DDRYGGY RSRS+ G +LYGKRPED   RFDS YDDG+GDGVYAYQGG
Sbjct: 181 SSVKFEQGGGFGDDRYGGYGRSRSEFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGG 240

Query: 250 KVEPYGARGTASKSSTWSAA-PAFDDYGRPINIPPKKDSSATSLKVVRATPKADAQEDVK 309
           KVEPYGARGTASKSSTWSAA PAFDDYGRPI+IPPKKD  A+SLKVVRA PK +AQEDVK
Sbjct: 241 KVEPYGARGTASKSSTWSAAAPAFDDYGRPISIPPKKDPPASSLKVVRAIPKVEAQEDVK 300

Query: 310 SWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSNRTLRIYPLETITRCEVYDSST 369
            WVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNS+RTLRIYPLETITRCEVYDSST
Sbjct: 301 DWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSST 360

Query: 370 LAFWSKSPVDIEPRRIRLQSNRYTTNTLLDTVTAATVQFKEMGGRSRPSDSFKAPEQPTE 429
           LAFWSKS VDIEPRRIRLQSN+YTTNTLLDTVTAATVQFKEMGGRSRP++SFKAPEQ TE
Sbjct: 361 LAFWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPAESFKAPEQATE 420

Query: 430 KKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIFCDKCTQ 489
           KKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCT+CGTDFGAFVR+HHCRNCGDIFCDKCTQ
Sbjct: 421 KKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGTDFGAFVRRHHCRNCGDIFCDKCTQ 480

Query: 490 GRTALTAEENVPQVRVCDRCMAEVTQRLANAKEMASKPAGLHSHEDLAKKLKDEMDKNRR 549
           GRT LTAEEN PQVRVCDRCMAEVTQRLANAKE+ASKP GLHSHEDLAKKLKDEM++NRR
Sbjct: 481 GRTTLTAEENAPQVRVCDRCMAEVTQRLANAKELASKPGGLHSHEDLAKKLKDEMERNRR 540

Query: 550 SSGSKSDGFGRRMKEVACPTCTVNLQVQVPSSGSETIECGVCQHPFLVSAH 597
           SSGSKSDG G+RMKEVACPTCTV+LQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 SSGSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 591

BLAST of MC11g0090 vs. NCBI nr
Match: KAA0063302.1 (protein FREE1 [Cucumis melo var. makuwa])

HSP 1 Score: 1065 bits (2754), Expect = 0.0
Identity = 529/593 (89.21%), Postives = 555/593 (93.59%), Query Frame = 0

Query: 10  MQHGDYGTAPYFQYPNLQNLSLNPSPNPVPTPPDRNQSAYASAPPFSTNYGGSDYPGYAS 69
           MQ+GDYGTAPYFQYPNLQNLSLNPSPNPVPTP DRN   YASAPPFSTNYG SDYP Y+ 
Sbjct: 1   MQNGDYGTAPYFQYPNLQNLSLNPSPNPVPTPSDRN---YASAPPFSTNYGASDYPAYSP 60

Query: 70  NYAHYSQNPDPVPASPTAPLYTPPSSNPNLQTFNPTPQPPAFPPFESHTPYQPPSQPQSY 129
           NY  YSQNPDPVPASPTAPLY PPSSNPNLQTFNPTPQPPAFPPFESH PYQ PSQ QSY
Sbjct: 61  NYPPYSQNPDPVPASPTAPLYNPPSSNPNLQTFNPTPQPPAFPPFESHVPYQSPSQSQSY 120

Query: 130 HSTYDQNQTAPNYAP----PPPSSIPVNPSSNSTPSPNSPFSSMYSAPFGSLPPPAYENP 189
           +STYDQ+QTAPNYAP    PPPSSIP+N SSNSTPSPNSP+SSMYSAPFGSL PP+YENP
Sbjct: 121 YSTYDQHQTAPNYAPTPPPPPPSSIPMNQSSNSTPSPNSPYSSMYSAPFGSLAPPSYENP 180

Query: 190 YESSVKFDQGGGYVDDRYGGYSRSRSDLGPDLYGKRPEDTISRFDSAYDDGFGDGVYAYQ 249
           YESSVKFDQGGGY DDRYGGY RSRSD G +LYGKRPED   RFDS YDDG+GDGVYAYQ
Sbjct: 181 YESSVKFDQGGGYGDDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQ 240

Query: 250 GGKVEPYGARGTASKSSTWSAA-PAFDDYGRPINIPPKKDSSATSLKVVRATPKADAQED 309
           GGK EPYGARGTASKSSTWSAA PAFDDYGRPI+IPPKK+S A+SLKVVRA PK +AQED
Sbjct: 241 GGKAEPYGARGTASKSSTWSAAAPAFDDYGRPISIPPKKESPASSLKVVRAIPKVEAQED 300

Query: 310 VKSWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSNRTLRIYPLETITRCEVYDS 369
            K+WVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNS+RTLRIYPLETITRCEVYDS
Sbjct: 301 AKNWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDS 360

Query: 370 STLAFWSKSPVDIEPRRIRLQSNRYTTNTLLDTVTAATVQFKEMGGRSRPSDSFKAPEQP 429
           STLAFWSKS VDIEPRRIRLQSN+YTTNTLLDTVTAATVQFKEMGGRSRP++SFKA EQ 
Sbjct: 361 STLAFWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPAESFKASEQA 420

Query: 430 TEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIFCDKC 489
           TEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCT+CGTDFGAFVR+HHCRNCGDIFCDKC
Sbjct: 421 TEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGTDFGAFVRRHHCRNCGDIFCDKC 480

Query: 490 TQGRTALTAEENVPQVRVCDRCMAEVTQRLANAKEMASKPAGLHSHEDLAKKLKDEMDKN 549
           TQGRTALTAEEN PQVRVCDRCMAEVTQRLANAK++ASKPAGLHSHEDLAKKLKDEM++N
Sbjct: 481 TQGRTALTAEENAPQVRVCDRCMAEVTQRLANAKDLASKPAGLHSHEDLAKKLKDEMERN 540

Query: 550 RRSSGSKSDGFGRRMKEVACPTCTVNLQVQVPSSGSETIECGVCQHPFLVSAH 597
           RRSSGSKSDG G+RMKEVACPTCTV+LQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 RRSSGSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 590

BLAST of MC11g0090 vs. NCBI nr
Match: XP_022941056.1 (protein FREE1-like [Cucurbita moschata])

HSP 1 Score: 1064 bits (2752), Expect = 0.0
Identity = 526/592 (88.85%), Postives = 547/592 (92.40%), Query Frame = 0

Query: 10  MQHGDYGTAPYFQYPNLQNLSLNPSPNPVPTPPDRNQSAYASAPPFSTNYGGSDYPGYAS 69
           MQ+GDYGT PYFQYPNLQNLSLNPSPNPV  PPDRNQSAYASAPPFSTNYGGSDYPGYA 
Sbjct: 1   MQNGDYGTPPYFQYPNLQNLSLNPSPNPVSPPPDRNQSAYASAPPFSTNYGGSDYPGYAP 60

Query: 70  NYAHYSQNPDPVPASPTAPLYTPPSSNPNLQTFNPTPQPPAFPPFESHTPYQPPSQPQSY 129
           NY  YSQNPDPVPASPTAPLY P SSNPNLQTFNPT QPPAFPPFESH PYQ PSQPQSY
Sbjct: 61  NYPPYSQNPDPVPASPTAPLYNPQSSNPNLQTFNPTSQPPAFPPFESHVPYQSPSQPQSY 120

Query: 130 HSTYDQNQTAPNYAPPPPSSIPVNPSSNSTPSPNSPFSSMYSAPFGSLPPPAYENPYESS 189
           +STYD +QTAPNYAPPPPSSIP N  SNSTPSPNS FSS+YSAPFGSL  P+YENPYESS
Sbjct: 121 NSTYDHHQTAPNYAPPPPSSIPSNQGSNSTPSPNSHFSSVYSAPFGSLAQPSYENPYESS 180

Query: 190 VKFDQGGGYVDDRYGGYSRSRSDLGPDLYGKRPEDTISRFDSAYDDGFGDGVYAYQGGKV 249
           VKFDQGGGY DDRYGGY RSRSDLGPDLYGKRPED  SRFDS YDDGFG+GVYAYQGGKV
Sbjct: 181 VKFDQGGGYADDRYGGYGRSRSDLGPDLYGKRPEDAASRFDSGYDDGFGEGVYAYQGGKV 240

Query: 250 EPYGARGTASKSSTWSAAPAFDDYGRPINIPPKKDSSATSLKVVRATPKADAQEDVKSWV 309
           EPYGARGTA KSSTWS  PAFDDYGRPINIP KKD   +SLKVVRA PK +AQEDVK+WV
Sbjct: 241 EPYGARGTAPKSSTWSTGPAFDDYGRPINIPSKKDPPTSSLKVVRAIPKVEAQEDVKNWV 300

Query: 310 QKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSNRTLRIYPLETITRCEVYDSSTLAF 369
           QKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNS+RTLRIYPLETITRCE+YDSSTLAF
Sbjct: 301 QKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEIYDSSTLAF 360

Query: 370 WSKSPVDIEPRRIRLQSNRYTTNTLLDTVTAATVQFKEMGGRSRPSDSFKAPEQPTEKKK 429
           WSKS VDIEPRRIRLQSN+YTTNTLLDTVTAATVQFKEMGGRSRPS+SFKAPEQP EKKK
Sbjct: 361 WSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPAEKKK 420

Query: 430 GLVDWVNLIKPGNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIFCDKCTQGRT 489
           GLVDWV L+KPGNEEKDHWVPDEAVTKCT+CGTDFGAFVR+HHCRNCGDIFCDKCTQGRT
Sbjct: 421 GLVDWVTLMKPGNEEKDHWVPDEAVTKCTACGTDFGAFVRRHHCRNCGDIFCDKCTQGRT 480

Query: 490 ALTAEENVPQVRVCDRCMAEVTQRLANAKEMASKPAGLHSHEDLAKKLKDEMDKNRRSSG 549
           ALTAEEN P VRVCDRCMAEVT RL NAKE+ASKPAGLHSHEDLAKKLKDEM++NR SSG
Sbjct: 481 ALTAEENAPPVRVCDRCMAEVTHRLTNAKELASKPAGLHSHEDLAKKLKDEMERNRWSSG 540

Query: 550 ----SKSDGFGRRMKEVACPTCTVNLQVQVPSSGSETIECGVCQHPFLVSAH 597
               SKSDG G+RMKEVACPTCTV+LQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 NATASKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 592

BLAST of MC11g0090 vs. NCBI nr
Match: TYK31475.1 (protein FREE1 [Cucumis melo var. makuwa])

HSP 1 Score: 1064 bits (2751), Expect = 0.0
Identity = 528/593 (89.04%), Postives = 555/593 (93.59%), Query Frame = 0

Query: 10  MQHGDYGTAPYFQYPNLQNLSLNPSPNPVPTPPDRNQSAYASAPPFSTNYGGSDYPGYAS 69
           MQ+GDYGTAPYFQYPNLQNLSLNPSPNPVPTP DRN   YASAPPFSTNYG SDYP Y+ 
Sbjct: 1   MQNGDYGTAPYFQYPNLQNLSLNPSPNPVPTPSDRN---YASAPPFSTNYGASDYPAYSP 60

Query: 70  NYAHYSQNPDPVPASPTAPLYTPPSSNPNLQTFNPTPQPPAFPPFESHTPYQPPSQPQSY 129
           NY  YSQNPDPVPASPTAPLY PPSSNPNLQTFNPTPQPPAFPPFESH PYQ PSQ QSY
Sbjct: 61  NYPPYSQNPDPVPASPTAPLYNPPSSNPNLQTFNPTPQPPAFPPFESHVPYQSPSQSQSY 120

Query: 130 HSTYDQNQTAPNYAP----PPPSSIPVNPSSNSTPSPNSPFSSMYSAPFGSLPPPAYENP 189
           +STYDQ+QTAPNYAP    PPPSSIP+N SSNSTPSPNSP+SSMYSAPFGSL PP+YENP
Sbjct: 121 YSTYDQHQTAPNYAPTPPPPPPSSIPMNQSSNSTPSPNSPYSSMYSAPFGSLAPPSYENP 180

Query: 190 YESSVKFDQGGGYVDDRYGGYSRSRSDLGPDLYGKRPEDTISRFDSAYDDGFGDGVYAYQ 249
           YESSVKFDQGGGY DDRYGGY RSRSD G +LYGKRPED   RFDS YDDG+GDGVYAYQ
Sbjct: 181 YESSVKFDQGGGYGDDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQ 240

Query: 250 GGKVEPYGARGTASKSSTWSAA-PAFDDYGRPINIPPKKDSSATSLKVVRATPKADAQED 309
           GGK EPYGARGT+SKSSTWSAA PAFDDYGRPI+IPPKK+S A+SLKVVRA PK +AQED
Sbjct: 241 GGKAEPYGARGTSSKSSTWSAAAPAFDDYGRPISIPPKKESPASSLKVVRAIPKVEAQED 300

Query: 310 VKSWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSNRTLRIYPLETITRCEVYDS 369
            K+WVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNS+RTLRIYPLETITRCEVYDS
Sbjct: 301 AKNWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDS 360

Query: 370 STLAFWSKSPVDIEPRRIRLQSNRYTTNTLLDTVTAATVQFKEMGGRSRPSDSFKAPEQP 429
           STLAFWSKS VDIEPRRIRLQSN+YTTNTLLDTVTAATVQFKEMGGRSRP++SFKA EQ 
Sbjct: 361 STLAFWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPAESFKASEQA 420

Query: 430 TEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIFCDKC 489
           TEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCT+CGTDFGAFVR+HHCRNCGDIFCDKC
Sbjct: 421 TEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGTDFGAFVRRHHCRNCGDIFCDKC 480

Query: 490 TQGRTALTAEENVPQVRVCDRCMAEVTQRLANAKEMASKPAGLHSHEDLAKKLKDEMDKN 549
           TQGRTALTAEEN PQVRVCDRCMAEVTQRLANAK++ASKPAGLHSHEDLAKKLKDEM++N
Sbjct: 481 TQGRTALTAEENAPQVRVCDRCMAEVTQRLANAKDLASKPAGLHSHEDLAKKLKDEMERN 540

Query: 550 RRSSGSKSDGFGRRMKEVACPTCTVNLQVQVPSSGSETIECGVCQHPFLVSAH 597
           RRSSGSKSDG G+RMKEVACPTCTV+LQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 RRSSGSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 590

BLAST of MC11g0090 vs. ExPASy TrEMBL
Match: A0A6J1C4P9 (protein FREE1 OS=Momordica charantia OX=3673 GN=LOC111008454 PE=4 SV=1)

HSP 1 Score: 1204 bits (3116), Expect = 0.0
Identity = 594/597 (99.50%), Postives = 595/597 (99.66%), Query Frame = 0

Query: 1   MANFSSASAMQHGDYGTAPYFQYPNLQNLSLNPSPNPVPTPPDRNQSAYASAPPFSTNYG 60
           MANFSSASAMQHGDYGTAPYFQYPNLQNLSLNPSPNPVPTPPDRNQSAYASAPPFSTNYG
Sbjct: 1   MANFSSASAMQHGDYGTAPYFQYPNLQNLSLNPSPNPVPTPPDRNQSAYASAPPFSTNYG 60

Query: 61  GSDYPGYASNYAHYSQNPDPVPASPTAPLYTPPSSNPNLQTFNPTPQPPAFPPFESHTPY 120
           GSDYPGYASNYAHYSQNPDPVPASPTAPL+TPPSSNPNLQTFNPTPQPPAFPPFESH PY
Sbjct: 61  GSDYPGYASNYAHYSQNPDPVPASPTAPLFTPPSSNPNLQTFNPTPQPPAFPPFESHAPY 120

Query: 121 QPPSQPQSYHSTYDQNQTAPNYAPPPPSSIPVNPSSNSTPSPNSPFSSMYSAPFGSLPPP 180
           QPPSQPQSYHSTYDQNQTAPNYAPPPPSSIPVNPSSNSTPSPNSPFSSMYSAPFGSLPPP
Sbjct: 121 QPPSQPQSYHSTYDQNQTAPNYAPPPPSSIPVNPSSNSTPSPNSPFSSMYSAPFGSLPPP 180

Query: 181 AYENPYESSVKFDQGGGYVDDRYGGYSRSRSDLGPDLYGKRPEDTISRFDSAYDDGFGDG 240
           AYENPYESSVKFDQGGGYVDDRYGGYSRSRSDLGPDLYGKRPEDTISRFDSAYDDGFGDG
Sbjct: 181 AYENPYESSVKFDQGGGYVDDRYGGYSRSRSDLGPDLYGKRPEDTISRFDSAYDDGFGDG 240

Query: 241 VYAYQGGKVEPYGARGTASKSSTWSAAPAFDDYGRPINIPPKKDSSATSLKVVRATPKAD 300
           VYAYQGGKVEPYGARGTASKSSTWSAAPAFDDYGRPINIPPKKDSSATSLKVVRATPKAD
Sbjct: 241 VYAYQGGKVEPYGARGTASKSSTWSAAPAFDDYGRPINIPPKKDSSATSLKVVRATPKAD 300

Query: 301 AQEDVKSWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSNRTLRIYPLETITRCE 360
           AQEDVKSWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSNRTLRIYPLETITRCE
Sbjct: 301 AQEDVKSWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSNRTLRIYPLETITRCE 360

Query: 361 VYDSSTLAFWSKSPVDIEPRRIRLQSNRYTTNTLLDTVTAATVQFKEMGGRSRPSDSFKA 420
           VYDSSTLAFWSKSPVDIEPRRIRLQSNRYTTNTLLDTVTAATVQFKEMGGRSRPSDSFKA
Sbjct: 361 VYDSSTLAFWSKSPVDIEPRRIRLQSNRYTTNTLLDTVTAATVQFKEMGGRSRPSDSFKA 420

Query: 421 PEQPTEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIF 480
           PEQPTEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIF
Sbjct: 421 PEQPTEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIF 480

Query: 481 CDKCTQGRTALTAEENVPQVRVCDRCMAEVTQRLANAKEMASKPAGLHSHEDLAKKLKDE 540
           CDKCTQGRTALTAEENVPQVRVCDRCMAEVTQRLANAKEMASKPAGLHSHEDLAKKLKDE
Sbjct: 481 CDKCTQGRTALTAEENVPQVRVCDRCMAEVTQRLANAKEMASKPAGLHSHEDLAKKLKDE 540

Query: 541 MDKNRRSSGSKSDGFGRRMKEVACPTCTVNLQVQVPSSGSETIECGVCQHPFLVSAH 597
           MDKNRRSSGSKSDGFG RMKEVACPTCTVNLQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 MDKNRRSSGSKSDGFGSRMKEVACPTCTVNLQVQVPSSGSETIECGVCQHPFLVSAH 597

BLAST of MC11g0090 vs. ExPASy TrEMBL
Match: A0A5A7V4X1 (Protein FREE1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold205G001250 PE=4 SV=1)

HSP 1 Score: 1065 bits (2754), Expect = 0.0
Identity = 529/593 (89.21%), Postives = 555/593 (93.59%), Query Frame = 0

Query: 10  MQHGDYGTAPYFQYPNLQNLSLNPSPNPVPTPPDRNQSAYASAPPFSTNYGGSDYPGYAS 69
           MQ+GDYGTAPYFQYPNLQNLSLNPSPNPVPTP DRN   YASAPPFSTNYG SDYP Y+ 
Sbjct: 1   MQNGDYGTAPYFQYPNLQNLSLNPSPNPVPTPSDRN---YASAPPFSTNYGASDYPAYSP 60

Query: 70  NYAHYSQNPDPVPASPTAPLYTPPSSNPNLQTFNPTPQPPAFPPFESHTPYQPPSQPQSY 129
           NY  YSQNPDPVPASPTAPLY PPSSNPNLQTFNPTPQPPAFPPFESH PYQ PSQ QSY
Sbjct: 61  NYPPYSQNPDPVPASPTAPLYNPPSSNPNLQTFNPTPQPPAFPPFESHVPYQSPSQSQSY 120

Query: 130 HSTYDQNQTAPNYAP----PPPSSIPVNPSSNSTPSPNSPFSSMYSAPFGSLPPPAYENP 189
           +STYDQ+QTAPNYAP    PPPSSIP+N SSNSTPSPNSP+SSMYSAPFGSL PP+YENP
Sbjct: 121 YSTYDQHQTAPNYAPTPPPPPPSSIPMNQSSNSTPSPNSPYSSMYSAPFGSLAPPSYENP 180

Query: 190 YESSVKFDQGGGYVDDRYGGYSRSRSDLGPDLYGKRPEDTISRFDSAYDDGFGDGVYAYQ 249
           YESSVKFDQGGGY DDRYGGY RSRSD G +LYGKRPED   RFDS YDDG+GDGVYAYQ
Sbjct: 181 YESSVKFDQGGGYGDDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQ 240

Query: 250 GGKVEPYGARGTASKSSTWSAA-PAFDDYGRPINIPPKKDSSATSLKVVRATPKADAQED 309
           GGK EPYGARGTASKSSTWSAA PAFDDYGRPI+IPPKK+S A+SLKVVRA PK +AQED
Sbjct: 241 GGKAEPYGARGTASKSSTWSAAAPAFDDYGRPISIPPKKESPASSLKVVRAIPKVEAQED 300

Query: 310 VKSWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSNRTLRIYPLETITRCEVYDS 369
            K+WVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNS+RTLRIYPLETITRCEVYDS
Sbjct: 301 AKNWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDS 360

Query: 370 STLAFWSKSPVDIEPRRIRLQSNRYTTNTLLDTVTAATVQFKEMGGRSRPSDSFKAPEQP 429
           STLAFWSKS VDIEPRRIRLQSN+YTTNTLLDTVTAATVQFKEMGGRSRP++SFKA EQ 
Sbjct: 361 STLAFWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPAESFKASEQA 420

Query: 430 TEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIFCDKC 489
           TEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCT+CGTDFGAFVR+HHCRNCGDIFCDKC
Sbjct: 421 TEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGTDFGAFVRRHHCRNCGDIFCDKC 480

Query: 490 TQGRTALTAEENVPQVRVCDRCMAEVTQRLANAKEMASKPAGLHSHEDLAKKLKDEMDKN 549
           TQGRTALTAEEN PQVRVCDRCMAEVTQRLANAK++ASKPAGLHSHEDLAKKLKDEM++N
Sbjct: 481 TQGRTALTAEENAPQVRVCDRCMAEVTQRLANAKDLASKPAGLHSHEDLAKKLKDEMERN 540

Query: 550 RRSSGSKSDGFGRRMKEVACPTCTVNLQVQVPSSGSETIECGVCQHPFLVSAH 597
           RRSSGSKSDG G+RMKEVACPTCTV+LQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 RRSSGSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 590

BLAST of MC11g0090 vs. ExPASy TrEMBL
Match: A0A6J1FK39 (protein FREE1-like OS=Cucurbita moschata OX=3662 GN=LOC111446458 PE=4 SV=1)

HSP 1 Score: 1064 bits (2752), Expect = 0.0
Identity = 526/592 (88.85%), Postives = 547/592 (92.40%), Query Frame = 0

Query: 10  MQHGDYGTAPYFQYPNLQNLSLNPSPNPVPTPPDRNQSAYASAPPFSTNYGGSDYPGYAS 69
           MQ+GDYGT PYFQYPNLQNLSLNPSPNPV  PPDRNQSAYASAPPFSTNYGGSDYPGYA 
Sbjct: 1   MQNGDYGTPPYFQYPNLQNLSLNPSPNPVSPPPDRNQSAYASAPPFSTNYGGSDYPGYAP 60

Query: 70  NYAHYSQNPDPVPASPTAPLYTPPSSNPNLQTFNPTPQPPAFPPFESHTPYQPPSQPQSY 129
           NY  YSQNPDPVPASPTAPLY P SSNPNLQTFNPT QPPAFPPFESH PYQ PSQPQSY
Sbjct: 61  NYPPYSQNPDPVPASPTAPLYNPQSSNPNLQTFNPTSQPPAFPPFESHVPYQSPSQPQSY 120

Query: 130 HSTYDQNQTAPNYAPPPPSSIPVNPSSNSTPSPNSPFSSMYSAPFGSLPPPAYENPYESS 189
           +STYD +QTAPNYAPPPPSSIP N  SNSTPSPNS FSS+YSAPFGSL  P+YENPYESS
Sbjct: 121 NSTYDHHQTAPNYAPPPPSSIPSNQGSNSTPSPNSHFSSVYSAPFGSLAQPSYENPYESS 180

Query: 190 VKFDQGGGYVDDRYGGYSRSRSDLGPDLYGKRPEDTISRFDSAYDDGFGDGVYAYQGGKV 249
           VKFDQGGGY DDRYGGY RSRSDLGPDLYGKRPED  SRFDS YDDGFG+GVYAYQGGKV
Sbjct: 181 VKFDQGGGYADDRYGGYGRSRSDLGPDLYGKRPEDAASRFDSGYDDGFGEGVYAYQGGKV 240

Query: 250 EPYGARGTASKSSTWSAAPAFDDYGRPINIPPKKDSSATSLKVVRATPKADAQEDVKSWV 309
           EPYGARGTA KSSTWS  PAFDDYGRPINIP KKD   +SLKVVRA PK +AQEDVK+WV
Sbjct: 241 EPYGARGTAPKSSTWSTGPAFDDYGRPINIPSKKDPPTSSLKVVRAIPKVEAQEDVKNWV 300

Query: 310 QKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSNRTLRIYPLETITRCEVYDSSTLAF 369
           QKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNS+RTLRIYPLETITRCE+YDSSTLAF
Sbjct: 301 QKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEIYDSSTLAF 360

Query: 370 WSKSPVDIEPRRIRLQSNRYTTNTLLDTVTAATVQFKEMGGRSRPSDSFKAPEQPTEKKK 429
           WSKS VDIEPRRIRLQSN+YTTNTLLDTVTAATVQFKEMGGRSRPS+SFKAPEQP EKKK
Sbjct: 361 WSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPAEKKK 420

Query: 430 GLVDWVNLIKPGNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIFCDKCTQGRT 489
           GLVDWV L+KPGNEEKDHWVPDEAVTKCT+CGTDFGAFVR+HHCRNCGDIFCDKCTQGRT
Sbjct: 421 GLVDWVTLMKPGNEEKDHWVPDEAVTKCTACGTDFGAFVRRHHCRNCGDIFCDKCTQGRT 480

Query: 490 ALTAEENVPQVRVCDRCMAEVTQRLANAKEMASKPAGLHSHEDLAKKLKDEMDKNRRSSG 549
           ALTAEEN P VRVCDRCMAEVT RL NAKE+ASKPAGLHSHEDLAKKLKDEM++NR SSG
Sbjct: 481 ALTAEENAPPVRVCDRCMAEVTHRLTNAKELASKPAGLHSHEDLAKKLKDEMERNRWSSG 540

Query: 550 ----SKSDGFGRRMKEVACPTCTVNLQVQVPSSGSETIECGVCQHPFLVSAH 597
               SKSDG G+RMKEVACPTCTV+LQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 NATASKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 592

BLAST of MC11g0090 vs. ExPASy TrEMBL
Match: A0A5D3E825 (Protein FREE1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G007910 PE=4 SV=1)

HSP 1 Score: 1064 bits (2751), Expect = 0.0
Identity = 528/593 (89.04%), Postives = 555/593 (93.59%), Query Frame = 0

Query: 10  MQHGDYGTAPYFQYPNLQNLSLNPSPNPVPTPPDRNQSAYASAPPFSTNYGGSDYPGYAS 69
           MQ+GDYGTAPYFQYPNLQNLSLNPSPNPVPTP DRN   YASAPPFSTNYG SDYP Y+ 
Sbjct: 1   MQNGDYGTAPYFQYPNLQNLSLNPSPNPVPTPSDRN---YASAPPFSTNYGASDYPAYSP 60

Query: 70  NYAHYSQNPDPVPASPTAPLYTPPSSNPNLQTFNPTPQPPAFPPFESHTPYQPPSQPQSY 129
           NY  YSQNPDPVPASPTAPLY PPSSNPNLQTFNPTPQPPAFPPFESH PYQ PSQ QSY
Sbjct: 61  NYPPYSQNPDPVPASPTAPLYNPPSSNPNLQTFNPTPQPPAFPPFESHVPYQSPSQSQSY 120

Query: 130 HSTYDQNQTAPNYAP----PPPSSIPVNPSSNSTPSPNSPFSSMYSAPFGSLPPPAYENP 189
           +STYDQ+QTAPNYAP    PPPSSIP+N SSNSTPSPNSP+SSMYSAPFGSL PP+YENP
Sbjct: 121 YSTYDQHQTAPNYAPTPPPPPPSSIPMNQSSNSTPSPNSPYSSMYSAPFGSLAPPSYENP 180

Query: 190 YESSVKFDQGGGYVDDRYGGYSRSRSDLGPDLYGKRPEDTISRFDSAYDDGFGDGVYAYQ 249
           YESSVKFDQGGGY DDRYGGY RSRSD G +LYGKRPED   RFDS YDDG+GDGVYAYQ
Sbjct: 181 YESSVKFDQGGGYGDDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQ 240

Query: 250 GGKVEPYGARGTASKSSTWSAA-PAFDDYGRPINIPPKKDSSATSLKVVRATPKADAQED 309
           GGK EPYGARGT+SKSSTWSAA PAFDDYGRPI+IPPKK+S A+SLKVVRA PK +AQED
Sbjct: 241 GGKAEPYGARGTSSKSSTWSAAAPAFDDYGRPISIPPKKESPASSLKVVRAIPKVEAQED 300

Query: 310 VKSWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSNRTLRIYPLETITRCEVYDS 369
            K+WVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNS+RTLRIYPLETITRCEVYDS
Sbjct: 301 AKNWVQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDS 360

Query: 370 STLAFWSKSPVDIEPRRIRLQSNRYTTNTLLDTVTAATVQFKEMGGRSRPSDSFKAPEQP 429
           STLAFWSKS VDIEPRRIRLQSN+YTTNTLLDTVTAATVQFKEMGGRSRP++SFKA EQ 
Sbjct: 361 STLAFWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPAESFKASEQA 420

Query: 430 TEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIFCDKC 489
           TEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCT+CGTDFGAFVR+HHCRNCGDIFCDKC
Sbjct: 421 TEKKKGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGTDFGAFVRRHHCRNCGDIFCDKC 480

Query: 490 TQGRTALTAEENVPQVRVCDRCMAEVTQRLANAKEMASKPAGLHSHEDLAKKLKDEMDKN 549
           TQGRTALTAEEN PQVRVCDRCMAEVTQRLANAK++ASKPAGLHSHEDLAKKLKDEM++N
Sbjct: 481 TQGRTALTAEENAPQVRVCDRCMAEVTQRLANAKDLASKPAGLHSHEDLAKKLKDEMERN 540

Query: 550 RRSSGSKSDGFGRRMKEVACPTCTVNLQVQVPSSGSETIECGVCQHPFLVSAH 597
           RRSSGSKSDG G+RMKEVACPTCTV+LQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 RRSSGSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 590

BLAST of MC11g0090 vs. ExPASy TrEMBL
Match: A0A6J1FEV1 (protein FREE1-like OS=Cucurbita moschata OX=3662 GN=LOC111443309 PE=4 SV=1)

HSP 1 Score: 1058 bits (2737), Expect = 0.0
Identity = 523/589 (88.79%), Postives = 548/589 (93.04%), Query Frame = 0

Query: 10  MQHGDYGTAPYFQYPNLQNLSLNPSPNPVPTPPDRNQSAYASAPPFSTNYGGSDYPGYAS 69
           MQ+GDYGTAPYFQY N QNL+LNPSPNPVP PPDRNQSAYASAPPFSTNY  SDYPGYA 
Sbjct: 1   MQNGDYGTAPYFQYQNFQNLTLNPSPNPVPIPPDRNQSAYASAPPFSTNYAASDYPGYAP 60

Query: 70  NYAHYSQNPDPVPASPTAPLYTPPSSNPNLQTFNPTPQPPAFPPFESHTPYQPPSQPQSY 129
            Y  YSQNPDP+PASPTAPL+  PSSN N QTF PTPQ PAFPPFESH PYQ PSQ QSY
Sbjct: 61  YYPPYSQNPDPLPASPTAPLFNSPSSNSNPQTFIPTPQSPAFPPFESHVPYQSPSQSQSY 120

Query: 130 HSTYDQNQTAPNYAPPPPSSIPVNPSSNSTPSPNSPFSSMYSAPFGSLPPPAYENPYESS 189
           HSTYDQ+QTAPN APPPPSSIP+N SSNSTP+ NSPFSSMYSAPFGSL PP+YENPYESS
Sbjct: 121 HSTYDQHQTAPNCAPPPPSSIPMNQSSNSTPTANSPFSSMYSAPFGSLAPPSYENPYESS 180

Query: 190 VKFDQGGGYVDDRYGGYSRSRSDLGPDLYGKRPEDTISRFDSAYDDGFGDGVYAYQGGKV 249
           VKFDQGGGY DDRYGGY RSRSD G +LYGKRPED   RFDS YDDG+GDGVYAYQGGKV
Sbjct: 181 VKFDQGGGYADDRYGGYGRSRSDFGSELYGKRPEDAAPRFDSGYDDGYGDGVYAYQGGKV 240

Query: 250 EPYGARGTASKSSTWSAA-PAFDDYGRPINIPPKKDSSATSLKVVRATPKADAQEDVKSW 309
           EPYGARGTASKSSTWSAA PAFDDYGRPINIPPKKD  A+SLKVVRA PK +AQEDVK+W
Sbjct: 241 EPYGARGTASKSSTWSAATPAFDDYGRPINIPPKKDPPASSLKVVRAIPKVEAQEDVKNW 300

Query: 310 VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSNRTLRIYPLETITRCEVYDSSTLA 369
           VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNS+RTLRIYPLETITRCEVYDSSTLA
Sbjct: 301 VQKFRVKLLAESGGQSTMDVLCQVGLDGIRMLDPNSSRTLRIYPLETITRCEVYDSSTLA 360

Query: 370 FWSKSPVDIEPRRIRLQSNRYTTNTLLDTVTAATVQFKEMGGRSRPSDSFKAPEQPTEKK 429
           FWSKS VDIEPRRIRLQSN+YTTNTLLDTVTAATVQFKEMGGRSRPS+SFKAPEQPTEKK
Sbjct: 361 FWSKSSVDIEPRRIRLQSNKYTTNTLLDTVTAATVQFKEMGGRSRPSESFKAPEQPTEKK 420

Query: 430 KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIFCDKCTQGR 489
           KGLVDWVNLIKPGNEEKDHWVPDEAVTKCT+CG+DFGAFVR+HHCRNCGDIFCDKCTQGR
Sbjct: 421 KGLVDWVNLIKPGNEEKDHWVPDEAVTKCTACGSDFGAFVRRHHCRNCGDIFCDKCTQGR 480

Query: 490 TALTAEENVPQVRVCDRCMAEVTQRLANAKEMASKPAGLHSHEDLAKKLKDEMDKNRRSS 549
           TALTAEEN PQVRVCDRCMAEVTQRLANAK++ S P GLHSHEDLAKKLKDEM++NRRSS
Sbjct: 481 TALTAEENAPQVRVCDRCMAEVTQRLANAKDLTSNPVGLHSHEDLAKKLKDEMERNRRSS 540

Query: 550 GSKSDGFGRRMKEVACPTCTVNLQVQVPSSGSETIECGVCQHPFLVSAH 597
           GSKSDG G+RMKEVACPTCTV+LQVQVPSSGSETIECGVCQHPFLVSAH
Sbjct: 541 GSKSDGSGKRMKEVACPTCTVHLQVQVPSSGSETIECGVCQHPFLVSAH 589

BLAST of MC11g0090 vs. TAIR 10
Match: AT1G20110.1 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 684.1 bits (1764), Expect = 1.0e-196
Identity = 383/623 (61.48%), Postives = 447/623 (71.75%), Query Frame = 0

Query: 10  MQHGDYGTAPYFQYPNLQNLSLNPSPNPVPTPP---------DRNQSAYASAPPFSTNYG 69
           MQ GDY +  + QY   QN + NP+PNP P+PP         D  ++ YASAPPF+  YG
Sbjct: 1   MQQGDYNSYYHHQYSQFQNPTPNPNPNPNPSPPAPATVAGPTDLTRNTYASAPPFTGGYG 60

Query: 70  GSDYPGYASNYAHYSQNPDPVPASPTAPLYTPPSS----------NPN-LQTFNPTPQPP 129
            +DY  Y+ NY  Y QN + VP  P+AP +T PS           NPN   TFN  P PP
Sbjct: 61  SADYSNYSQNYTPYGQNSEHVP--PSAPSFTSPSQPPPSPPATSLNPNSYSTFNQPPPPP 120

Query: 130 AFPP--------FESHTPYQPPSQPQSYHSTYDQNQTAPNYAPPPPSSIPVNPSSNSTPS 189
              P        F+S  PYQ P+    Y+S YDQ+QT+   + PPPSS    P+ N  P+
Sbjct: 121 TIHPQPLSSYGSFDSTAPYQQPTSQHMYYSPYDQHQTSGYSSAPPPSSA---PAPNPNPA 180

Query: 190 PNSPFSSMYSAP----FGSLPPPAYENPYESSVKFDQGGGYVDDRYGGYSRSRSDLGPDL 249
           P S  SS+YSAP     GS  PP+YE P   SVKFDQ G      Y GY+RSRSDLG DL
Sbjct: 181 PYS--SSLYSAPPYSSGGSSIPPSYEKP---SVKFDQSG------YDGYNRSRSDLGSDL 240

Query: 250 YGKRPEDTISRFDSAYDDGFGDGVYAYQGGKVEPYGARGTASKSSTWSAAPAFDDYGRPI 309
           YGKR +   S    A++D +GDGVYAYQGGKVEPYG+RGTA KSS    +  FDDYGR I
Sbjct: 241 YGKRSD---SGEYPAFEDSYGDGVYAYQGGKVEPYGSRGTAPKSSN---STLFDDYGRSI 300

Query: 310 NIPPK-KDS--SATSLKVVRATPKADAQEDVKSWVQKFRVKLLAESGGQSTMDVLCQVGL 369
           +     +DS  S+ S K+VRA PKAD QED    VQKFRVKLLAE+ GQ+T DVLCQ+GL
Sbjct: 301 SFSSSGRDSSVSSNSAKIVRAVPKADVQEDSTGGVQKFRVKLLAETYGQTTTDVLCQIGL 360

Query: 370 DGIRMLDPNSNRTLRIYPLETITRCEVYDSSTLAFWSKSPVDIEPRRIRLQSNRYTTNTL 429
           DG+RMLDP+++RTLRIYPLE ITRCE  DSS LAFWSK+PVDIE +RIRLQSN YTTNTL
Sbjct: 361 DGLRMLDPSTSRTLRIYPLENITRCEKLDSSILAFWSKTPVDIEAKRIRLQSNSYTTNTL 420

Query: 430 LDTVTAATVQFKEMGGRSRPSDSFKAPEQPTEKKKGLVDWVNLIKPGNEEKDHWVPDEAV 489
           LDTVTAA  Q KE+GG SRP  S K  EQ  EKKKGL DW+N+IKP NEEKDHWVPDEAV
Sbjct: 421 LDTVTAAMFQAKEIGGSSRPPTSGKLIEQTAEKKKGLGDWMNIIKPVNEEKDHWVPDEAV 480

Query: 490 TKCTSCGTDFGAFVRKHHCRNCGDIFCDKCTQGRTALTAEENVPQVRVCDRCMAEVTQRL 549
           +KCTSCG+DFGAF+R+HHCRNCGD+FCDKCTQGR ALTAE+N PQVRVCDRCMAEV+QRL
Sbjct: 481 SKCTSCGSDFGAFIRRHHCRNCGDVFCDKCTQGRIALTAEDNAPQVRVCDRCMAEVSQRL 540

Query: 550 ANAKEMASKPAGLHSHEDLAKKLKDEMDKNRRSSGSKSDGFGRRMKEVACPTCTVNLQVQ 598
           +NAKE   +   L SHEDLA+KL++EM++NR+SS    +G GRRMKEVACPTCTV+LQVQ
Sbjct: 541 SNAKETTGRNVSLQSHEDLARKLQEEMERNRKSSSGLREGSGRRMKEVACPTCTVHLQVQ 600

BLAST of MC11g0090 vs. TAIR 10
Match: AT3G14270.1 (phosphatidylinositol-4-phosphate 5-kinase family protein )

HSP 1 Score: 66.6 bits (161), Expect = 7.6e-11
Identity = 30/98 (30.61%), Postives = 49/98 (50.00%), Query Frame = 0

Query: 439 KPGNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIFCDKCTQGRTAL------T 498
           +P    +D W+PD++   C  C   F    R+HHCR+CG +FC KCT            T
Sbjct: 27  EPATVSRDFWMPDQSCRVCYECDCQFTLINRRHHCRHCGRVFCGKCTANSIPFAPSDLRT 86

Query: 499 AEENVPQVRVCDRCMAEVTQ-----RLANAKEMASKPA 526
             E+  ++RVC+ C  +  Q      ++N  E+++ P+
Sbjct: 87  PREDWERIRVCNYCFRQWEQGDGGPHVSNITELSTSPS 124

BLAST of MC11g0090 vs. TAIR 10
Match: AT4G33240.1 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 62.4 bits (150), Expect = 1.4e-09
Identity = 34/107 (31.78%), Postives = 47/107 (43.93%), Query Frame = 0

Query: 427 KKKGLVDWVNLIKP-----GNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIFC 486
           K  G VD V    P      N  +D W+PD++   C  C   F  F R+HHCR CG +FC
Sbjct: 7   KAPGFVDIVKSWIPRKSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCRLCGRVFC 66

Query: 487 DKCTQGRTALTAE------ENVPQVRVCDRCMAEVTQRLANAKEMAS 523
            KC        ++      E   ++RVC+ C  +  Q +      AS
Sbjct: 67  AKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNGAS 113

BLAST of MC11g0090 vs. TAIR 10
Match: AT4G33240.2 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 62.4 bits (150), Expect = 1.4e-09
Identity = 34/107 (31.78%), Postives = 47/107 (43.93%), Query Frame = 0

Query: 427 KKKGLVDWVNLIKP-----GNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIFC 486
           K  G VD V    P      N  +D W+PD++   C  C   F  F R+HHCR CG +FC
Sbjct: 7   KAPGFVDIVKSWIPRKSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCRLCGRVFC 66

Query: 487 DKCTQGRTALTAE------ENVPQVRVCDRCMAEVTQRLANAKEMAS 523
            KC        ++      E   ++RVC+ C  +  Q +      AS
Sbjct: 67  AKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNGAS 113

BLAST of MC11g0090 vs. TAIR 10
Match: AT4G33240.3 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 62.4 bits (150), Expect = 1.4e-09
Identity = 34/107 (31.78%), Postives = 47/107 (43.93%), Query Frame = 0

Query: 427 KKKGLVDWVNLIKP-----GNEEKDHWVPDEAVTKCTSCGTDFGAFVRKHHCRNCGDIFC 486
           K  G VD V    P      N  +D W+PD++   C  C   F  F R+HHCR CG +FC
Sbjct: 7   KAPGFVDIVKSWIPRKSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCRLCGRVFC 66

Query: 487 DKCTQGRTALTAE------ENVPQVRVCDRCMAEVTQRLANAKEMAS 523
            KC        ++      E   ++RVC+ C  +  Q +      AS
Sbjct: 67  AKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNGAS 113