MC11g0077 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g0077 vs. ExPASy Swiss-Prot
Match: F4IME2 (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 PE=2 SV=2)

HSP 1 Score: 637.1 bits (1642), Expect = 2.4e-181
Identity = 335/663 (50.53%), Postives = 469/663 (70.74%), Query Frame = 0

Query: 61  EVLRCSSNASF-RGCSWSSPISKKSRLMDPPEEDS-------SQKLETAVSEEGKNQDE- 120
           EV+RC+SN SF R     S +  +SRL DPP E+        S +L++ +  +   +D+ 
Sbjct: 222 EVVRCTSNMSFQRKSELISRVKTRSRLQDPPREEETPYSGWRSGQLKSGLLADIDEEDDP 281

Query: 121 --TEDMPEEYNQLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVL 180
              ED+P+EY + KL   ++LQW+  V +I  L C+  +   K+  +W+L LWKWE+ +L
Sbjct: 282 LAEEDVPDEYKRGKLDAITLLQWLSLVAIIAALACSLSIQSWKKVRVWNLHLWKWEVFLL 341

Query: 181 ALICGRLISTWAVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNE 240
            LICGRL+S W + ++V  IERNFLLRKRVLYFVYG+RRAV+NCLWL L+LL WHF+F++
Sbjct: 342 VLICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGVRRAVQNCLWLGLVLLAWHFLFDK 401

Query: 241 KVQRTTRSKILPYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYV 300
           KVQR TRS+ LPY+TKIL+CFL+   +WL+KTL VK+LAS FHV+ YF+R++EALF+QYV
Sbjct: 402 KVQRETRSRFLPYVTKILVCFLLSTILWLIKTLVVKVLASSFHVSTYFDRIQEALFNQYV 461

Query: 301 IVTLSGSPLFERSNTE-------DMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLK 360
           I TLSG P+ E S  E       D +   QNA G    P     +C+         R + 
Sbjct: 462 IETLSGPPMIEMSRIEEEEERAQDEIFKMQNA-GANLPPD----LCAAAFPPGKSGRVMN 521

Query: 361 PE------SSRQDEEIPVDQLNKLNQHNISAWNMRRMMNIVRNGALLTLDEKILSET-KE 420
           P+       S  D  I ++ L+++N  NISAWNM+R+M IVRN +L TLDE++L  T ++
Sbjct: 522 PKLSPIIPKSTTDNGISMEHLHRMNHKNISAWNMKRLMKIVRNVSLTTLDEQMLESTYED 581

Query: 421 EYLLQIRSERQAKEAAGNIFRRVSRRGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQC 480
           E   QIRSE++AK AA  IF+ V +RG+++I L+D+MRF+ ++EA+ TM LF    E + 
Sbjct: 582 ESTRQIRSEKEAKAAARKIFKNVEQRGAKYIYLEDLMRFLREDEAMKTMGLFEGAPENKR 641

Query: 481 IDEFSFREWMVNAFNQRKVLVLSLNDANTAVDGLHNLLNIFVAVTILVIWLIILGTPILH 540
           I + + + W+VNAF +R+ L L+LND  TAV+ LH+++NI  A+ I+VIWL++L      
Sbjct: 642 ISKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMINIVTAIVIVVIWLVLLEIASSK 701

Query: 541 FLIFISSQLLLLVFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTV 600
            L+F+SSQ++LL FIFGNT KTVFE+IIFLF++HP+DVGDRCE+D VQ+VVEEM ILTTV
Sbjct: 702 VLLFVSSQVVLLAFIFGNTVKTVFESIIFLFIVHPYDVGDRCEIDSVQLVVEEMNILTTV 761

Query: 601 FLRYDNQKITYPNSVLATKAIGNYYRSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIES 660
           FLRYDN KI YPNS+L  K+I NYYRSPDMG+ ++F VHI+TP +K+S+IK++I+ YI++
Sbjct: 762 FLRYDNLKIMYPNSLLWQKSINNYYRSPDMGDAIEFCVHITTPLEKISVIKQRISNYIDN 821

Query: 661 KSEEWHPAPVVTMKDVEDLEKVKMSVCVTHKMNHQDMGERWNRRAALVEEIVKIFRQLDD 699
           K E W+P   + +KD+EDL  V++++   H++NHQDM ERW RRA LVEE++KI  +LD 
Sbjct: 822 KPEYWYPQAKIIVKDLEDLHIVRLAIWPCHRINHQDMAERWTRRAVLVEEVIKILLELDI 879

BLAST of MC11g0077 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 6.9e-181
Identity = 348/701 (49.64%), Postives = 479/701 (68.33%), Query Frame = 0

Query: 49  ENRRPSSASGGGEVLRCSSNAS--FRGCSWSSPISKKSRLMDPPEED------------- 108
           ++R  ++ +  GEV++CS N +   R  S    +  +SRL DPP                
Sbjct: 138 DSRSNTNNNDDGEVVKCSGNNAPIQRSSSTLLKMRTRSRLSDPPTPQLPPQTADMKSGRI 197

Query: 109 --SSQKLETAVSEEGKNQDE--------TEDMPEEYNQLKLSPFSVLQWVIFVIVIVGLI 168
             S Q       +  K Q E         ED+PEEY + KLS + VL+W+  +++I G +
Sbjct: 198 PKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFV 257

Query: 169 CNRWVPILKRKSIWDLPLWKWELTVLALICGRLISTWAVGLIVKIIERNFLLRKRVLYFV 228
           C   +P L++K +W+L LWKWE  VL LICGRL+S+W V ++V  IERNFLLRKRVLYFV
Sbjct: 258 CTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFV 317

Query: 229 YGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRSKILPYITKILICFLVGASIWLLKTLA 288
           YG+R+AV+NCLWL L+LL WHF+F+EKV +   +K L  +TKI +C LVG  +WL+KTL 
Sbjct: 318 YGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCLLVGFLLWLVKTLL 377

Query: 289 VKILASFFHVNNYFERLKEALFSQYVIVTLSGSPLFERSNTED-------MVKDNQN--- 348
           VK+LAS FH++ YF+R++E+LF+QYVI TLSG PL E    E+        VK  QN   
Sbjct: 378 VKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKFQNPGG 437

Query: 349 ---ADGREESP-KSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQLNKLNQHNISAWN 408
                G ++SP K+G+      +++  G          +++ I +D L+KLN  N+SAW 
Sbjct: 438 VEIQSGAQKSPMKTGKSPFLSHVLSNGGG------GGGENKGITIDSLHKLNPKNVSAWK 497

Query: 409 MRRMMNIVRNGALLTLDEKILSET-KEEYLLQIRSERQAKEAAGNIFRRVSRRGSEFINL 468
           M+R+MNI+RNG+L TLDE++   +  ++   QIRSE +AK AA  IF  V++ GS+FI  
Sbjct: 498 MKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEAKLAARKIFHNVAKPGSKFIYA 557

Query: 469 DDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVLSLNDANTAVDG 528
           +D+MRF+  +EAL T+SLF   +ET  I + S + W+VNAF +R+ L L+LND  TAV+ 
Sbjct: 558 NDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNR 617

Query: 529 LHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIFGNTCKTVFEAIIFLFVM 588
           LH ++NI V + ILVIWLIILG     FL+ +SSQ++++ FIFGN CK VFE+II+LFV+
Sbjct: 618 LHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVI 677

Query: 589 HPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIGNYYRSPDMGEG 648
           HPFDVGDRCE+DGVQMVVEEM ILTTVFLR+DNQK+ YPNS+L TK+IGNYYRSPDMG+G
Sbjct: 678 HPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDG 737

Query: 649 VDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKVKMSVCVTHKMN 705
           ++F +HI+TP++K+ LIK++IT YIE K + W+PAP++  KD+E L  V+++V  TH+MN
Sbjct: 738 IEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMN 797

BLAST of MC11g0077 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 7.6e-172
Identity = 322/704 (45.74%), Postives = 462/704 (65.62%), Query Frame = 0

Query: 42  GKESSDSENRRPSSASGGGEVLRCSSNASFRGCSWSSPISKKSRLMDPPEED-------- 101
           G  S D ENR       G EV++C+SN S         +  +SRLMDPP           
Sbjct: 151 GDSSEDEENR-----VDGSEVVKCTSNRSTMRTKTLMKMKTRSRLMDPPTPTYPDMVSGR 210

Query: 102 -----------SSQKLETAVSEEGKNQD--------ETEDMPEEYNQLKLSPFSVLQWVI 161
                      S +  +     +G ++D          ED+PE   + K+  + +++W+ 
Sbjct: 211 TPRSGNLNPGFSGRNTKPGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIF 270

Query: 162 FVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLISTWAVGLIVKIIERNFL 221
            +++I  LIC+  +P L+ K++WDL LWKWE+ VL LICGRL+S+W V L V  +E NFL
Sbjct: 271 LILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFL 330

Query: 222 LRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRSKILPYITKILICFLVGA 281
            RK+VLYFVYG+R+ V+NCLWL L+L+ WHF+F++KV+R  RS +L Y+TK+LIC LV  
Sbjct: 331 WRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAV 390

Query: 282 SIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSPLFERSNTEDMVKDNQNA 341
            IWL+KTL VK+LAS FH++ YF+R++E+LF+QYVI TLSG P  E    E+ V ++   
Sbjct: 391 IIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIHIEEEKVANDVKT 450

Query: 342 ---DGREESPKSGRIIC--------SGRLVNCDGSRQLKPESSRQ-------DEEIPVDQ 401
               GR+ SP   + +         SGRL          P  SR        +E I +D 
Sbjct: 451 FEIVGRKLSPLGPKAVSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDH 510

Query: 402 LNKLNQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEE--YLLQIRSERQAKEAAGNI 461
           L ++N  N+SAW M+++MN+++ G L TLDE+I   T +E     QIRSE +AK AA  I
Sbjct: 511 LQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKI 570

Query: 462 FRRVSRRGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKV 521
           F+ V+  GS +I ++D MRF++++E+   M LF   +E   I +   + W+VNAF +R+ 
Sbjct: 571 FQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRA 630

Query: 522 LVLSLNDANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIFGNT 581
           L L+LND  TAV+ LH ++++ V++ IL+IWL+ILG     FL+ ISSQLLL+VF+FGN+
Sbjct: 631 LALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNS 690

Query: 582 CKTVFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATK 641
           CKT+FEA+IF+FVMHPFDVGDRCE+DGVQM+VEEM ILTTVFLR+DNQKI YPNS+L TK
Sbjct: 691 CKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTK 750

Query: 642 AIGNYYRSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDL 699
            I NYYRSPDM + ++F+VHI+TP +K + ++++I  Y+++K + WHP+P++  +D+  L
Sbjct: 751 PIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGL 810

BLAST of MC11g0077 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 2.2e-171
Identity = 336/707 (47.52%), Postives = 463/707 (65.49%), Query Frame = 0

Query: 35  AQTMRRRGKE-----SSDSENRRPSSASGGGEVLRCSSNASFRGCSWSSPISKKSRLMDP 94
           A  +RRR        SSD E R    A    EVL+C S          S    KSRL DP
Sbjct: 151 ADAVRRRQNRTSLGGSSDEEGRNRDEA----EVLKCGSKKPM-----LSRNKTKSRLQDP 210

Query: 95  P----------EEDSSQKLETAVS-----------------EEGKNQDE--TEDMPEEYN 154
           P          E  S ++     S                 EE + +D    ED+PEE+ 
Sbjct: 211 PTPTHPAIDKTEMKSGRRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFK 270

Query: 155 QLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLISTW 214
           + KLS +  L+W+  V+++  L+C+  +  L+RK+ W L LWKWE+TVL LICGRL+S+W
Sbjct: 271 RDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSW 330

Query: 215 AVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRSKIL 274
            V +IV ++E+NF  RKRVLYFVYG+R++V+NCLWL L+LL WHF+F++KV+R TRS  L
Sbjct: 331 IVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTAL 390

Query: 275 PYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSPLFE 334
            Y+T++L+C LV   IWL+KT+ VK+LAS FH++ YF+R++E+LF+QYVI TLSG PL E
Sbjct: 391 RYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLME 450

Query: 335 RSNTEDM-------VKDNQNADGREESPKSGRIICSGRLV-NCDGSRQLKPESSRQDEEI 394
               E+        VK  +   G +  P     + S   V    G  ++  +     E I
Sbjct: 451 IQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGI 510

Query: 395 PVDQLNKLNQHNISAWNMRRMMNIVRNGALLTLDEKILSETKE-EYLLQIRSERQAKEAA 454
            +DQL ++N  N+SAWNM+R+MNI+  GA+ TLD+ +   T+E E    IRSE +AK AA
Sbjct: 511 RIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSEYEAKCAA 570

Query: 455 GNIFRRVSRRGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQ 514
             IF  V+  GS +I L+D +RF+ +EEA   M+LF   +E+  I +   + W+V AF +
Sbjct: 571 RKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRE 630

Query: 515 RKVLVLSLNDANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIF 574
           R+ L L+LND  TAVD LH ++N+ + + I++IWL+ILG     FL+ +SSQLLL+ F+F
Sbjct: 631 RRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVF 690

Query: 575 GNTCKTVFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVL 634
           GN+CKT+FEAIIFLFVMHPFDVGDRCE+DGVQ+VVEEM ILTTVFLRYDNQKI YPNSVL
Sbjct: 691 GNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVL 750

Query: 635 ATKAIGNYYRSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDV 694
            TK I NYYRSPDMG+ V+F VHI+TP +K++ IK++I  Y+++K + W+PAP++    +
Sbjct: 751 GTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSM 810

Query: 695 EDLEKVKMSVCVTHKMNHQDMGERWNRRAALVEEIVKIFRQLDDEHQ 699
           +DL  VK++V +TH+MNHQDMGER+ RR  L+EE+ K  R+LD E++
Sbjct: 811 DDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYR 848

BLAST of MC11g0077 vs. ExPASy Swiss-Prot
Match: F4IME1 (Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana OX=3702 GN=MSL7 PE=2 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 3.3e-159
Identity = 322/721 (44.66%), Postives = 477/721 (66.16%), Query Frame = 0

Query: 7   VLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGGEVLRCS 66
           V  KI+S G   +     ++       S+ TMR   ++  + E         G  V+RCS
Sbjct: 109 VFFKINSSGTN-NMSGSVRSCTSSTSFSSATMRLNLEQQLEDE---------GEVVVRCS 168

Query: 67  SNASFRGCSWSSPISKKSRLMDPPEED---------SSQKLETAV----SEEGKNQDET- 126
              S R     S    +SRL+DPP+E+         +S +L + +    S++ + +D++ 
Sbjct: 169 ---SVRKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSS 228

Query: 127 --EDMPEEYNQLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLA 186
             ED+P EY +LK+   ++LQW+  + ++V L+ +  +   +  ++W L LWKWE+ +L 
Sbjct: 229 AEEDVPVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLV 288

Query: 187 LICGRLISTWAVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEK 246
           LICGRL+S   + +IV  IERNFLLRKRVLYFVYG++ AV+NCLWL L+LL WHF+F++K
Sbjct: 289 LICGRLVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKK 348

Query: 247 VQRTTRSKILPYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVI 306
           V++ T+S +L  ++KIL+CFL+   +WL+KTL VK+LAS FHV+ YF+R++EALF  Y+I
Sbjct: 349 VEKETQSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLI 408

Query: 307 VTLSGSPLFERSNTEDMVKDNQNADGREESPKSGRIIC------------SGRLVNCDGS 366
            TLSG P+ E S  E+  ++++  D   +  K G  +             SG  +N   S
Sbjct: 409 ETLSGPPMLELSRIEE--EEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTMNMKFS 468

Query: 367 RQLKPESSRQDEEIPVDQLNKLNQHNISAWNMRRMMNIVRNGALLTLDEKILSET-KEEY 426
             +    S  D  I +D L+K+NQ N+SAWNM+R+M IVRN +L TLDE+ L  T ++E 
Sbjct: 469 PIIPKTGS--DNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCEDES 528

Query: 427 LLQIRSERQAKEAAGNIFRRVSRRGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCID 486
             QIRSE++AK AA  IF+ V++ G++ I L+D+MRF+  +EA+ TM LF     T+ I 
Sbjct: 529 TRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTKKIT 588

Query: 487 EFSFREWMVNAFNQRKVLVLSLNDANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFL 546
           + + + W+VNAF +R+ L L+LND  TAV+ LH++++   A+ I+VIWLI+L      +L
Sbjct: 589 KSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATSKYL 648

Query: 547 IFISSQLLLLVFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFL 606
           +F++SQ++LL F+FGN+ KTVFE+IIFLF++HP+DVGDR  +D V+MVVEEM ILTTVFL
Sbjct: 649 LFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTTVFL 708

Query: 607 RYDNQKITYPNSVLATKAIGNYYRSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKS 666
           R DN KI YPN +L  KAI NY RSPDMG+ V   VHI+TP +K++ IK++I+ YI+SK 
Sbjct: 709 RADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYIDSKP 768

Query: 667 EEWHPAPVVTMKDVEDLEKVKMSVCVTHKMNHQDMGERWNRRAALVEEIVKIFRQLDDEH 699
           E W+P   V +KDVEDL  V++++ + HK+NHQ+MGER+ RRA L+EE++KI  +LD ++
Sbjct: 769 EYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELDIQY 812

BLAST of MC11g0077 vs. NCBI nr
Match: XP_022140870.1 (mechanosensitive ion channel protein 6-like isoform X1 [Momordica charantia])

HSP 1 Score: 1368 bits (3542), Expect = 0.0
Identity = 709/709 (100.00%), Postives = 709/709 (100.00%), Query Frame = 0

Query: 1   MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG 60
           MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG
Sbjct: 1   MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG 60

Query: 61  EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEGKNQDETEDMPEEYN 120
           EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEGKNQDETEDMPEEYN
Sbjct: 61  EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEGKNQDETEDMPEEYN 120

Query: 121 QLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLISTW 180
           QLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLISTW
Sbjct: 121 QLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLISTW 180

Query: 181 AVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRSKIL 240
           AVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRSKIL
Sbjct: 181 AVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRSKIL 240

Query: 241 PYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSPLFE 300
           PYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSPLFE
Sbjct: 241 PYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSPLFE 300

Query: 301 RSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQLNKL 360
           RSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQLNKL
Sbjct: 301 RSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQLNKL 360

Query: 361 NQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAAGNIFRRVSR 420
           NQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAAGNIFRRVSR
Sbjct: 361 NQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAAGNIFRRVSR 420

Query: 421 RGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVLSLN 480
           RGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVLSLN
Sbjct: 421 RGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVLSLN 480

Query: 481 DANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIFGNTCKTVFE 540
           DANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIFGNTCKTVFE
Sbjct: 481 DANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIFGNTCKTVFE 540

Query: 541 AIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIGNYY 600
           AIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIGNYY
Sbjct: 541 AIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIGNYY 600

Query: 601 RSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKVKMS 660
           RSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKVKMS
Sbjct: 601 RSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKVKMS 660

Query: 661 VCVTHKMNHQDMGERWNRRAALVEEIVKIFRQLDDEHQENVQNLASGAT 709
           VCVTHKMNHQDMGERWNRRAALVEEIVKIFRQLDDEHQENVQNLASGAT
Sbjct: 661 VCVTHKMNHQDMGERWNRRAALVEEIVKIFRQLDDEHQENVQNLASGAT 709

BLAST of MC11g0077 vs. NCBI nr
Match: XP_022140879.1 (mechanosensitive ion channel protein 6-like isoform X2 [Momordica charantia])

HSP 1 Score: 1294 bits (3348), Expect = 0.0
Identity = 675/709 (95.20%), Postives = 675/709 (95.20%), Query Frame = 0

Query: 1   MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG 60
           MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG
Sbjct: 1   MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG 60

Query: 61  EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEGKNQDETEDMPEEYN 120
           EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEGKNQDETEDMPEEYN
Sbjct: 61  EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEGKNQDETEDMPEEYN 120

Query: 121 QLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLISTW 180
           QLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLISTW
Sbjct: 121 QLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLISTW 180

Query: 181 AVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRSKIL 240
           AVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRSKIL
Sbjct: 181 AVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRSKIL 240

Query: 241 PYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSPLFE 300
           PYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSPLFE
Sbjct: 241 PYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSPLFE 300

Query: 301 RSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQLNKL 360
           RSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQLNKL
Sbjct: 301 RSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQLNKL 360

Query: 361 NQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAAGNIFRRVSR 420
           NQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAAGNIFRRVSR
Sbjct: 361 NQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAAGNIFRRVSR 420

Query: 421 RGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVLSLN 480
           RGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVLSLN
Sbjct: 421 RGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVLSLN 480

Query: 481 DANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIFGNTCKTVFE 540
           DANTAVDGLHNLLNIFVAVTIL                                  TVFE
Sbjct: 481 DANTAVDGLHNLLNIFVAVTIL----------------------------------TVFE 540

Query: 541 AIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIGNYY 600
           AIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIGNYY
Sbjct: 541 AIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIGNYY 600

Query: 601 RSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKVKMS 660
           RSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKVKMS
Sbjct: 601 RSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKVKMS 660

Query: 661 VCVTHKMNHQDMGERWNRRAALVEEIVKIFRQLDDEHQENVQNLASGAT 709
           VCVTHKMNHQDMGERWNRRAALVEEIVKIFRQLDDEHQENVQNLASGAT
Sbjct: 661 VCVTHKMNHQDMGERWNRRAALVEEIVKIFRQLDDEHQENVQNLASGAT 675

BLAST of MC11g0077 vs. NCBI nr
Match: XP_022975440.1 (mechanosensitive ion channel protein 8-like [Cucurbita maxima])

HSP 1 Score: 1098 bits (2840), Expect = 0.0
Identity = 563/693 (81.24%), Postives = 630/693 (90.91%), Query Frame = 0

Query: 1   MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG 60
           MAP+EDV+LK++SFG+G DKE  EK FQCLPERS QTMRRRGKE S+ ENR+ SS S GG
Sbjct: 1   MAPKEDVILKVNSFGHG-DKELVEKKFQCLPERSLQTMRRRGKEFSEPENRQLSSGSSGG 60

Query: 61  EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEGKN---QDETEDMPE 120
           EVLRCSSN+SF G SWSSPISKKSRLMDPPE+DSSQKLETAV +EG N   QD TEDMPE
Sbjct: 61  EVLRCSSNSSFPGSSWSSPISKKSRLMDPPEDDSSQKLETAVKDEGNNNNNQDGTEDMPE 120

Query: 121 EYNQLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLI 180
           EYNQLKLSP    Q V+FV++IV LICNRW+ ILKR++ W LPLWKWELTVLA+ICG ++
Sbjct: 121 EYNQLKLSPLLGFQLVVFVLIIVSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMV 180

Query: 181 STWAVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRS 240
           S+ AVGL+VKIIE NFLLRKRVLYFVYGLR+AV+NCLW+SL+LLVWHFIFN+KVQR T+S
Sbjct: 181 SSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQS 240

Query: 241 KILPYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSP 300
           KILPY+TKILICFLV A+IWLLKTL +KILASFFHVNNYFERLKEALFSQYVIVTLSG P
Sbjct: 241 KILPYVTKILICFLVAATIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLP 300

Query: 301 LFERSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQL 360
           LFER N+EDM+KD Q ADG+ ES KSGR++CSGRLVNC+GSRQLK ES R+DEEIPVD+L
Sbjct: 301 LFERPNSEDMMKDEQIADGKAESSKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDEL 360

Query: 361 NKLNQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAAGNIFRR 420
           NKLNQ NISAWNMRRMMNIVRNGALLTLDEKILSETKE+ LLQIRSERQAKEAAG IFRR
Sbjct: 361 NKLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETKEQSLLQIRSERQAKEAAGRIFRR 420

Query: 421 VSRRGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVL 480
           V++RGS+ I ++DVMRFMNKEEAL+TMSLFG  AETQCIDE SFREWMVNAF QRKVLVL
Sbjct: 421 VAQRGSDCIYIEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVL 480

Query: 481 SLNDANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIFGNTCKT 540
           SLNDANTAVD LHNLLN+FVA+TILVIWLI+ GTP+LHFLIFI+SQLLLL FIFGNTCKT
Sbjct: 481 SLNDANTAVDDLHNLLNVFVALTILVIWLIVFGTPVLHFLIFITSQLLLLAFIFGNTCKT 540

Query: 541 VFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIG 600
           VFEAIIFLFVMHPFDVGDRCEVDGV+MVVEEM+ILTTVFLRYDNQ+I+YPNSVLATKAIG
Sbjct: 541 VFEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIG 600

Query: 601 NYYRSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKV 660
           NYYRSPDMGEGVDFY+HISTP +KV+L+KE+ITRY+ES  E+WHP P + MKDVEDLEKV
Sbjct: 601 NYYRSPDMGEGVDFYIHISTPWEKVALLKERITRYVESMGEQWHPDPQIVMKDVEDLEKV 660

Query: 661 KMSVCVTHKMNHQDMGERWNRRAALVEEIVKIF 690
           +MSV +THK+NHQ+M ERWNRRAALV+EIV I 
Sbjct: 661 RMSVGLTHKLNHQNMKERWNRRAALVQEIVTIL 692

BLAST of MC11g0077 vs. NCBI nr
Match: XP_022936287.1 (mechanosensitive ion channel protein 8-like [Cucurbita moschata])

HSP 1 Score: 1098 bits (2839), Expect = 0.0
Identity = 563/693 (81.24%), Postives = 628/693 (90.62%), Query Frame = 0

Query: 1   MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG 60
           MAP+EDV+LK++SFGNG DKE  EK FQCLPERS QTMRRRGKE S+ ENR+PSS S GG
Sbjct: 1   MAPKEDVILKVNSFGNG-DKELVEKKFQCLPERSLQTMRRRGKEFSEPENRQPSSGSSGG 60

Query: 61  EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEGKN---QDETEDMPE 120
           EVLRCSSN SF G SWSSPISKKSRLMDPPE+DSSQKLETAV +EG N   QD TEDMPE
Sbjct: 61  EVLRCSSNYSFSGSSWSSPISKKSRLMDPPEDDSSQKLETAVKDEGNNNNNQDGTEDMPE 120

Query: 121 EYNQLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLI 180
           EYNQLKLSP    Q V+FV++IV LICNRW+ ILKR++ W LPLWKWELTVLA+ICG ++
Sbjct: 121 EYNQLKLSPLLGFQLVVFVLIIVSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMV 180

Query: 181 STWAVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRS 240
           S+ AVGL+VKIIE NFLLRKRVLYFVYGLR+AV+NCLW+SL+LLVWHFIFN+KVQR T+S
Sbjct: 181 SSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQS 240

Query: 241 KILPYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSP 300
           KILPY+TKILICFLV ASIWLLKTL +KILASFFHVNNYFERLKEALFSQYVIVTLSG P
Sbjct: 241 KILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLP 300

Query: 301 LFERSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQL 360
           LFER N+EDM+KD Q  DG+ ES KSGR++CSGRLVNC+G RQLK ES R+DEEIPVD+L
Sbjct: 301 LFERPNSEDMMKDEQIGDGKAESSKSGRVVCSGRLVNCNGWRQLKVESKRKDEEIPVDEL 360

Query: 361 NKLNQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAAGNIFRR 420
           NKLNQ NISAWNMRRMMNIVRNGALLTLDEKILSET+E+ LLQIR+ERQAKEAAG IFRR
Sbjct: 361 NKLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRR 420

Query: 421 VSRRGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVL 480
           V++RGS+ I ++DVMRFMNKEEAL+TMSLFG  AETQCIDE SFREWMVNAF QRKVLVL
Sbjct: 421 VAQRGSDCIYIEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVL 480

Query: 481 SLNDANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIFGNTCKT 540
           SLNDANTAVD LHNLLN+FVA+TILVIWLII GTP+LHFLIFI+SQLLLL FIFGNTCKT
Sbjct: 481 SLNDANTAVDDLHNLLNVFVALTILVIWLIIFGTPVLHFLIFITSQLLLLAFIFGNTCKT 540

Query: 541 VFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIG 600
           VFEAIIFLFVMHPFDVGDRCEVDGV+MVVEEM+ILTTVFLRYDNQ+I+YPNSVLATKAIG
Sbjct: 541 VFEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIG 600

Query: 601 NYYRSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKV 660
           NYYRSPDMGEGVDFY+HISTP +KV+L+KE+ITRY+ES  E+WHP P + MKDVEDLEKV
Sbjct: 601 NYYRSPDMGEGVDFYIHISTPWEKVALLKERITRYVESMGEQWHPDPQIVMKDVEDLEKV 660

Query: 661 KMSVCVTHKMNHQDMGERWNRRAALVEEIVKIF 690
           +MSV +THK+NHQ+M ERWNRRAALV+EIV I 
Sbjct: 661 RMSVGLTHKLNHQNMKERWNRRAALVQEIVTIL 692

BLAST of MC11g0077 vs. NCBI nr
Match: XP_023535846.1 (mechanosensitive ion channel protein 8-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023535847.1 mechanosensitive ion channel protein 8-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1093 bits (2827), Expect = 0.0
Identity = 561/693 (80.95%), Postives = 627/693 (90.48%), Query Frame = 0

Query: 1   MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG 60
           MAP+EDV+LK++SF NG DKE  EK FQCLPERS QTMRRRGKE S+ +NR+PSS S GG
Sbjct: 1   MAPKEDVILKVNSFRNG-DKELVEKKFQCLPERSLQTMRRRGKEFSEPDNRQPSSGSSGG 60

Query: 61  EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEGKN---QDETEDMPE 120
           EVLRCSSN SF G SWSSPISKKSRLMDPPE+DSSQKLETAV +EG N   QD TEDMPE
Sbjct: 61  EVLRCSSNYSFSGSSWSSPISKKSRLMDPPEDDSSQKLETAVKDEGNNNDNQDGTEDMPE 120

Query: 121 EYNQLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLI 180
           EYNQLKLSP    Q V+FV++IV LICNRW+ ILKR++ W LPLWKWELTVLA+ICG ++
Sbjct: 121 EYNQLKLSPLLGFQLVVFVLIIVSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMV 180

Query: 181 STWAVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRS 240
           S+ AVGL+VKIIE NFLLRKRVLYFVYGLR+AV+NCLW SL+LLVWHFIFN+KVQR T+S
Sbjct: 181 SSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWTSLILLVWHFIFNDKVQRKTQS 240

Query: 241 KILPYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSP 300
           KILPY+TKILICFLV ASIWLLKTL +KILASFFHVNNYFERLKEALFSQYVIVTLSG P
Sbjct: 241 KILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLP 300

Query: 301 LFERSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQL 360
           LFER N+EDM+KD Q  DG+ ES KSGR++CSGRLVNC+GSRQLK ES R+DEEIPVD+L
Sbjct: 301 LFERPNSEDMMKDEQIDDGKAESSKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDEL 360

Query: 361 NKLNQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAAGNIFRR 420
           NKLNQ NISAWNMRRMMNIVRNGALLTLDEKILSET+E+ LLQIR+ERQAKEAAG IFRR
Sbjct: 361 NKLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRR 420

Query: 421 VSRRGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVL 480
           V++RGS+ I ++DVMRFMNKEEAL+TMSLFG  AETQCIDE SFREWMVNAF QRKVLVL
Sbjct: 421 VAQRGSDCIYIEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVL 480

Query: 481 SLNDANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIFGNTCKT 540
           SLNDANTAVD LHNLLN+FVA+TILVIWLII GTP+LHFLIFI+SQLLLL FIFGNTCKT
Sbjct: 481 SLNDANTAVDDLHNLLNVFVALTILVIWLIIFGTPVLHFLIFITSQLLLLAFIFGNTCKT 540

Query: 541 VFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIG 600
           VFEAIIFLFVMHPFDVGDRCEVDGV+MVVEEM+ILTTVFLRYDNQ+I+YPNSVLATKAIG
Sbjct: 541 VFEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIG 600

Query: 601 NYYRSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKV 660
           NYYRSPDMGEGVDFY+HISTP +KV+L+KE+ITRY+ES  E+WHP P + MKDVEDLEKV
Sbjct: 601 NYYRSPDMGEGVDFYIHISTPWEKVALLKERITRYVESMGEQWHPDPQIVMKDVEDLEKV 660

Query: 661 KMSVCVTHKMNHQDMGERWNRRAALVEEIVKIF 690
           +MSV +TH++NHQ+M ERWNRRAALV+EIV I 
Sbjct: 661 RMSVGLTHRLNHQNMKERWNRRAALVQEIVTIL 692

BLAST of MC11g0077 vs. ExPASy TrEMBL
Match: A0A6J1CGD4 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111011429 PE=3 SV=1)

HSP 1 Score: 1368 bits (3542), Expect = 0.0
Identity = 709/709 (100.00%), Postives = 709/709 (100.00%), Query Frame = 0

Query: 1   MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG 60
           MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG
Sbjct: 1   MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG 60

Query: 61  EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEGKNQDETEDMPEEYN 120
           EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEGKNQDETEDMPEEYN
Sbjct: 61  EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEGKNQDETEDMPEEYN 120

Query: 121 QLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLISTW 180
           QLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLISTW
Sbjct: 121 QLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLISTW 180

Query: 181 AVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRSKIL 240
           AVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRSKIL
Sbjct: 181 AVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRSKIL 240

Query: 241 PYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSPLFE 300
           PYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSPLFE
Sbjct: 241 PYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSPLFE 300

Query: 301 RSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQLNKL 360
           RSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQLNKL
Sbjct: 301 RSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQLNKL 360

Query: 361 NQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAAGNIFRRVSR 420
           NQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAAGNIFRRVSR
Sbjct: 361 NQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAAGNIFRRVSR 420

Query: 421 RGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVLSLN 480
           RGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVLSLN
Sbjct: 421 RGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVLSLN 480

Query: 481 DANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIFGNTCKTVFE 540
           DANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIFGNTCKTVFE
Sbjct: 481 DANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIFGNTCKTVFE 540

Query: 541 AIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIGNYY 600
           AIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIGNYY
Sbjct: 541 AIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIGNYY 600

Query: 601 RSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKVKMS 660
           RSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKVKMS
Sbjct: 601 RSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKVKMS 660

Query: 661 VCVTHKMNHQDMGERWNRRAALVEEIVKIFRQLDDEHQENVQNLASGAT 709
           VCVTHKMNHQDMGERWNRRAALVEEIVKIFRQLDDEHQENVQNLASGAT
Sbjct: 661 VCVTHKMNHQDMGERWNRRAALVEEIVKIFRQLDDEHQENVQNLASGAT 709

BLAST of MC11g0077 vs. ExPASy TrEMBL
Match: A0A6J1CH06 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111011429 PE=3 SV=1)

HSP 1 Score: 1294 bits (3348), Expect = 0.0
Identity = 675/709 (95.20%), Postives = 675/709 (95.20%), Query Frame = 0

Query: 1   MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG 60
           MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG
Sbjct: 1   MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG 60

Query: 61  EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEGKNQDETEDMPEEYN 120
           EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEGKNQDETEDMPEEYN
Sbjct: 61  EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEGKNQDETEDMPEEYN 120

Query: 121 QLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLISTW 180
           QLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLISTW
Sbjct: 121 QLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLISTW 180

Query: 181 AVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRSKIL 240
           AVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRSKIL
Sbjct: 181 AVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRSKIL 240

Query: 241 PYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSPLFE 300
           PYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSPLFE
Sbjct: 241 PYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSPLFE 300

Query: 301 RSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQLNKL 360
           RSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQLNKL
Sbjct: 301 RSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQLNKL 360

Query: 361 NQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAAGNIFRRVSR 420
           NQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAAGNIFRRVSR
Sbjct: 361 NQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAAGNIFRRVSR 420

Query: 421 RGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVLSLN 480
           RGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVLSLN
Sbjct: 421 RGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVLSLN 480

Query: 481 DANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIFGNTCKTVFE 540
           DANTAVDGLHNLLNIFVAVTIL                                  TVFE
Sbjct: 481 DANTAVDGLHNLLNIFVAVTIL----------------------------------TVFE 540

Query: 541 AIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIGNYY 600
           AIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIGNYY
Sbjct: 541 AIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIGNYY 600

Query: 601 RSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKVKMS 660
           RSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKVKMS
Sbjct: 601 RSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKVKMS 660

Query: 661 VCVTHKMNHQDMGERWNRRAALVEEIVKIFRQLDDEHQENVQNLASGAT 709
           VCVTHKMNHQDMGERWNRRAALVEEIVKIFRQLDDEHQENVQNLASGAT
Sbjct: 661 VCVTHKMNHQDMGERWNRRAALVEEIVKIFRQLDDEHQENVQNLASGAT 675

BLAST of MC11g0077 vs. ExPASy TrEMBL
Match: A0A6J1IGQ2 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111474765 PE=3 SV=1)

HSP 1 Score: 1098 bits (2840), Expect = 0.0
Identity = 563/693 (81.24%), Postives = 630/693 (90.91%), Query Frame = 0

Query: 1   MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG 60
           MAP+EDV+LK++SFG+G DKE  EK FQCLPERS QTMRRRGKE S+ ENR+ SS S GG
Sbjct: 1   MAPKEDVILKVNSFGHG-DKELVEKKFQCLPERSLQTMRRRGKEFSEPENRQLSSGSSGG 60

Query: 61  EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEGKN---QDETEDMPE 120
           EVLRCSSN+SF G SWSSPISKKSRLMDPPE+DSSQKLETAV +EG N   QD TEDMPE
Sbjct: 61  EVLRCSSNSSFPGSSWSSPISKKSRLMDPPEDDSSQKLETAVKDEGNNNNNQDGTEDMPE 120

Query: 121 EYNQLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLI 180
           EYNQLKLSP    Q V+FV++IV LICNRW+ ILKR++ W LPLWKWELTVLA+ICG ++
Sbjct: 121 EYNQLKLSPLLGFQLVVFVLIIVSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMV 180

Query: 181 STWAVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRS 240
           S+ AVGL+VKIIE NFLLRKRVLYFVYGLR+AV+NCLW+SL+LLVWHFIFN+KVQR T+S
Sbjct: 181 SSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQS 240

Query: 241 KILPYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSP 300
           KILPY+TKILICFLV A+IWLLKTL +KILASFFHVNNYFERLKEALFSQYVIVTLSG P
Sbjct: 241 KILPYVTKILICFLVAATIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLP 300

Query: 301 LFERSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQL 360
           LFER N+EDM+KD Q ADG+ ES KSGR++CSGRLVNC+GSRQLK ES R+DEEIPVD+L
Sbjct: 301 LFERPNSEDMMKDEQIADGKAESSKSGRVVCSGRLVNCNGSRQLKVESKRKDEEIPVDEL 360

Query: 361 NKLNQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAAGNIFRR 420
           NKLNQ NISAWNMRRMMNIVRNGALLTLDEKILSETKE+ LLQIRSERQAKEAAG IFRR
Sbjct: 361 NKLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETKEQSLLQIRSERQAKEAAGRIFRR 420

Query: 421 VSRRGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVL 480
           V++RGS+ I ++DVMRFMNKEEAL+TMSLFG  AETQCIDE SFREWMVNAF QRKVLVL
Sbjct: 421 VAQRGSDCIYIEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVL 480

Query: 481 SLNDANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIFGNTCKT 540
           SLNDANTAVD LHNLLN+FVA+TILVIWLI+ GTP+LHFLIFI+SQLLLL FIFGNTCKT
Sbjct: 481 SLNDANTAVDDLHNLLNVFVALTILVIWLIVFGTPVLHFLIFITSQLLLLAFIFGNTCKT 540

Query: 541 VFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIG 600
           VFEAIIFLFVMHPFDVGDRCEVDGV+MVVEEM+ILTTVFLRYDNQ+I+YPNSVLATKAIG
Sbjct: 541 VFEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIG 600

Query: 601 NYYRSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKV 660
           NYYRSPDMGEGVDFY+HISTP +KV+L+KE+ITRY+ES  E+WHP P + MKDVEDLEKV
Sbjct: 601 NYYRSPDMGEGVDFYIHISTPWEKVALLKERITRYVESMGEQWHPDPQIVMKDVEDLEKV 660

Query: 661 KMSVCVTHKMNHQDMGERWNRRAALVEEIVKIF 690
           +MSV +THK+NHQ+M ERWNRRAALV+EIV I 
Sbjct: 661 RMSVGLTHKLNHQNMKERWNRRAALVQEIVTIL 692

BLAST of MC11g0077 vs. ExPASy TrEMBL
Match: A0A6J1F811 (Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC111442956 PE=3 SV=1)

HSP 1 Score: 1098 bits (2839), Expect = 0.0
Identity = 563/693 (81.24%), Postives = 628/693 (90.62%), Query Frame = 0

Query: 1   MAPREDVLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGG 60
           MAP+EDV+LK++SFGNG DKE  EK FQCLPERS QTMRRRGKE S+ ENR+PSS S GG
Sbjct: 1   MAPKEDVILKVNSFGNG-DKELVEKKFQCLPERSLQTMRRRGKEFSEPENRQPSSGSSGG 60

Query: 61  EVLRCSSNASFRGCSWSSPISKKSRLMDPPEEDSSQKLETAVSEEGKN---QDETEDMPE 120
           EVLRCSSN SF G SWSSPISKKSRLMDPPE+DSSQKLETAV +EG N   QD TEDMPE
Sbjct: 61  EVLRCSSNYSFSGSSWSSPISKKSRLMDPPEDDSSQKLETAVKDEGNNNNNQDGTEDMPE 120

Query: 121 EYNQLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLI 180
           EYNQLKLSP    Q V+FV++IV LICNRW+ ILKR++ W LPLWKWELTVLA+ICG ++
Sbjct: 121 EYNQLKLSPLLGFQLVVFVLIIVSLICNRWISILKRETFWGLPLWKWELTVLAVICGHMV 180

Query: 181 STWAVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRS 240
           S+ AVGL+VKIIE NFLLRKRVLYFVYGLR+AV+NCLW+SL+LLVWHFIFN+KVQR T+S
Sbjct: 181 SSMAVGLMVKIIEMNFLLRKRVLYFVYGLRKAVKNCLWMSLILLVWHFIFNDKVQRKTQS 240

Query: 241 KILPYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSP 300
           KILPY+TKILICFLV ASIWLLKTL +KILASFFHVNNYFERLKEALFSQYVIVTLSG P
Sbjct: 241 KILPYVTKILICFLVAASIWLLKTLVIKILASFFHVNNYFERLKEALFSQYVIVTLSGLP 300

Query: 301 LFERSNTEDMVKDNQNADGREESPKSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQL 360
           LFER N+EDM+KD Q  DG+ ES KSGR++CSGRLVNC+G RQLK ES R+DEEIPVD+L
Sbjct: 301 LFERPNSEDMMKDEQIGDGKAESSKSGRVVCSGRLVNCNGWRQLKVESKRKDEEIPVDEL 360

Query: 361 NKLNQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEEYLLQIRSERQAKEAAGNIFRR 420
           NKLNQ NISAWNMRRMMNIVRNGALLTLDEKILSET+E+ LLQIR+ERQAKEAAG IFRR
Sbjct: 361 NKLNQKNISAWNMRRMMNIVRNGALLTLDEKILSETEEQSLLQIRNERQAKEAAGRIFRR 420

Query: 421 VSRRGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVL 480
           V++RGS+ I ++DVMRFMNKEEAL+TMSLFG  AETQCIDE SFREWMVNAF QRKVLVL
Sbjct: 421 VAQRGSDCIYIEDVMRFMNKEEALVTMSLFGTTAETQCIDESSFREWMVNAFKQRKVLVL 480

Query: 481 SLNDANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIFGNTCKT 540
           SLNDANTAVD LHNLLN+FVA+TILVIWLII GTP+LHFLIFI+SQLLLL FIFGNTCKT
Sbjct: 481 SLNDANTAVDDLHNLLNVFVALTILVIWLIIFGTPVLHFLIFITSQLLLLAFIFGNTCKT 540

Query: 541 VFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIG 600
           VFEAIIFLFVMHPFDVGDRCEVDGV+MVVEEM+ILTTVFLRYDNQ+I+YPNSVLATKAIG
Sbjct: 541 VFEAIIFLFVMHPFDVGDRCEVDGVEMVVEEMKILTTVFLRYDNQRISYPNSVLATKAIG 600

Query: 601 NYYRSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKV 660
           NYYRSPDMGEGVDFY+HISTP +KV+L+KE+ITRY+ES  E+WHP P + MKDVEDLEKV
Sbjct: 601 NYYRSPDMGEGVDFYIHISTPWEKVALLKERITRYVESMGEQWHPDPQIVMKDVEDLEKV 660

Query: 661 KMSVCVTHKMNHQDMGERWNRRAALVEEIVKIF 690
           +MSV +THK+NHQ+M ERWNRRAALV+EIV I 
Sbjct: 661 RMSVGLTHKLNHQNMKERWNRRAALVQEIVTIL 692

BLAST of MC11g0077 vs. ExPASy TrEMBL
Match: A0A6J5TPN6 (Mechanosensitive ion channel protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS7634 PE=3 SV=1)

HSP 1 Score: 784 bits (2024), Expect = 4.80e-275
Identity = 431/749 (57.54%), Postives = 558/749 (74.50%), Query Frame = 0

Query: 1   MAPRE-DVLLKIDSFGNGADKESEEKNFQCLP---------ERSAQTMRRRGKESSDSEN 60
           MA RE  V++KID+ G    KE+ + N +  P         E+  ++ RRR KE   ++ 
Sbjct: 1   MAQREGQVVVKIDNCGASCSKETTKINGEASPTTTTVGGSPEKPTESFRRRSKELPLTD- 60

Query: 61  RRPSSASGGGEVLRCSSNASFRGCSWSSPISK-KSRLMDPPEEDSSQKLETAVSEE--GK 120
               S SGG EVLRCSSNASF   SW  P+SK KSRL+DPPEE   +    A S    GK
Sbjct: 61  --APSNSGGAEVLRCSSNASFSRNSWKPPMSKTKSRLLDPPEESCLKSDRVAGSGRALGK 120

Query: 121 NQD------ETEDMPEEYNQLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPL 180
           N D      + ED+PEEY ++K S  ++LQWV  V VI  L+CN W+PI+KR+++WDLPL
Sbjct: 121 NDDNALDDADIEDIPEEYKRIKFSALTLLQWVSLVFVIAALVCNLWIPIIKRQTLWDLPL 180

Query: 181 WKWELTVLALICGRLISTWAVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLL 240
           WKWEL VLALICGRL+S W + ++V  +ERNFLLRKRVLYFVYGLR++V+NCLWL L+L+
Sbjct: 181 WKWELLVLALICGRLVSGWGIRVVVFFVERNFLLRKRVLYFVYGLRKSVQNCLWLGLVLV 240

Query: 241 VWHFIFNEKVQRTTRSKILPYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLK 300
           VWHFIF++KV+  T+S+ILPY+TK+LICFLVG  IWLLKT+ VK+LA  FHVN +FER++
Sbjct: 241 VWHFIFDKKVEEKTQSRILPYVTKVLICFLVGTLIWLLKTILVKVLALSFHVNAFFERIQ 300

Query: 301 EALFSQYVIVTLSGSPLFERSNTEDM------VKDNQNADG---RE------ESPKSGRI 360
           EALF+QYVI TLSG PLFER +TE+       +++ QNA     RE      +  +SGR+
Sbjct: 301 EALFNQYVIETLSGPPLFERKHTEEEEKVAAEIREFQNAGATMPRELRASLLQRARSGRV 360

Query: 361 ICSGRLVNC---DGSRQLKPESSRQDEEIPVDQLNKLNQHNISAWNMRRMMNIVRNGALL 420
           I SGR  +       R  +P S  QDEEIPVD L KLNQ N+SAWNMRRM+NI+R+G+L 
Sbjct: 361 IGSGRQNSPRVGKSPRVSRPTSGSQDEEIPVDHLQKLNQKNVSAWNMRRMVNIIRHGSLT 420

Query: 421 TLDEKIL-SETKEEYLLQIRSERQAKEAAGNIFRRVSRRGSEFINLDDVMRFMNKEEALM 480
           TLDE+IL S+ +++  L+I++E QAKEAA  IF +V++ GS +I L+D+M FM+K+EAL 
Sbjct: 421 TLDEQILNSDIEDDSSLEIKTECQAKEAAKKIFLKVAKPGSSYIYLEDLMPFMHKDEALK 480

Query: 481 TMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVLSLNDANTAVDGLHNLLNIFVAVTIL 540
           T+ LFG   E+  I + + R+W+V+AF +R+ L LSLND  TAVD LHN+LNI VAV I+
Sbjct: 481 TIHLFGAANESDRISKSALRDWVVSAFRERRALALSLNDTKTAVDELHNILNIIVAVIIV 540

Query: 541 VIWLIILGTPILHFLIFISSQLLLLVFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEVDGV 600
           +IWLIILG P+ HFL+ ISSQLLL+VFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEV+GV
Sbjct: 541 IIWLIILGIPVTHFLLLISSQLLLVVFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEVEGV 600

Query: 601 QMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIGNYYRSPDMGEGVDFYVHISTPSKKV 660
           QMVVEEM ILTTVFL++D+QKI YPNS+LATK I NY+RSPDMG+ VDF VHISTP +K+
Sbjct: 601 QMVVEEMNILTTVFLKFDHQKIIYPNSILATKPIANYHRSPDMGDAVDFCVHISTPLEKL 660

Query: 661 SLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKVKMSVCVTHKMNHQDMGERWNRRAAL 706
           +++KE+I  YIES+S+ W+ AP++ M+DVEDL K+K+SV  THKMNHQDM  RW RR+ L
Sbjct: 661 AIMKERIQGYIESRSDHWYTAPMLIMRDVEDLNKLKISVWPTHKMNHQDMLGRWTRRSLL 720

BLAST of MC11g0077 vs. TAIR 10
Match: AT1G78610.1 (mechanosensitive channel of small conductance-like 6 )

HSP 1 Score: 635.6 bits (1638), Expect = 4.9e-182
Identity = 348/701 (49.64%), Postives = 479/701 (68.33%), Query Frame = 0

Query: 49  ENRRPSSASGGGEVLRCSSNAS--FRGCSWSSPISKKSRLMDPPEED------------- 108
           ++R  ++ +  GEV++CS N +   R  S    +  +SRL DPP                
Sbjct: 138 DSRSNTNNNDDGEVVKCSGNNAPIQRSSSTLLKMRTRSRLSDPPTPQLPPQTADMKSGRI 197

Query: 109 --SSQKLETAVSEEGKNQDE--------TEDMPEEYNQLKLSPFSVLQWVIFVIVIVGLI 168
             S Q       +  K Q E         ED+PEEY + KLS + VL+W+  +++I G +
Sbjct: 198 PKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFV 257

Query: 169 CNRWVPILKRKSIWDLPLWKWELTVLALICGRLISTWAVGLIVKIIERNFLLRKRVLYFV 228
           C   +P L++K +W+L LWKWE  VL LICGRL+S+W V ++V  IERNFLLRKRVLYFV
Sbjct: 258 CTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFV 317

Query: 229 YGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRSKILPYITKILICFLVGASIWLLKTLA 288
           YG+R+AV+NCLWL L+LL WHF+F+EKV +   +K L  +TKI +C LVG  +WL+KTL 
Sbjct: 318 YGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCLLVGFLLWLVKTLL 377

Query: 289 VKILASFFHVNNYFERLKEALFSQYVIVTLSGSPLFERSNTED-------MVKDNQN--- 348
           VK+LAS FH++ YF+R++E+LF+QYVI TLSG PL E    E+        VK  QN   
Sbjct: 378 VKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKFQNPGG 437

Query: 349 ---ADGREESP-KSGRIICSGRLVNCDGSRQLKPESSRQDEEIPVDQLNKLNQHNISAWN 408
                G ++SP K+G+      +++  G          +++ I +D L+KLN  N+SAW 
Sbjct: 438 VEIQSGAQKSPMKTGKSPFLSHVLSNGGG------GGGENKGITIDSLHKLNPKNVSAWK 497

Query: 409 MRRMMNIVRNGALLTLDEKILSET-KEEYLLQIRSERQAKEAAGNIFRRVSRRGSEFINL 468
           M+R+MNI+RNG+L TLDE++   +  ++   QIRSE +AK AA  IF  V++ GS+FI  
Sbjct: 498 MKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSEFEAKLAARKIFHNVAKPGSKFIYA 557

Query: 469 DDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKVLVLSLNDANTAVDG 528
           +D+MRF+  +EAL T+SLF   +ET  I + S + W+VNAF +R+ L L+LND  TAV+ 
Sbjct: 558 NDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNR 617

Query: 529 LHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIFGNTCKTVFEAIIFLFVM 588
           LH ++NI V + ILVIWLIILG     FL+ +SSQ++++ FIFGN CK VFE+II+LFV+
Sbjct: 618 LHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVI 677

Query: 589 HPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATKAIGNYYRSPDMGEG 648
           HPFDVGDRCE+DGVQMVVEEM ILTTVFLR+DNQK+ YPNS+L TK+IGNYYRSPDMG+G
Sbjct: 678 HPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDG 737

Query: 649 VDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDLEKVKMSVCVTHKMN 705
           ++F +HI+TP++K+ LIK++IT YIE K + W+PAP++  KD+E L  V+++V  TH+MN
Sbjct: 738 IEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMN 797

BLAST of MC11g0077 vs. TAIR 10
Match: AT1G53470.1 (mechanosensitive channel of small conductance-like 4 )

HSP 1 Score: 605.5 bits (1560), Expect = 5.4e-173
Identity = 322/704 (45.74%), Postives = 462/704 (65.62%), Query Frame = 0

Query: 42  GKESSDSENRRPSSASGGGEVLRCSSNASFRGCSWSSPISKKSRLMDPPEED-------- 101
           G  S D ENR       G EV++C+SN S         +  +SRLMDPP           
Sbjct: 151 GDSSEDEENR-----VDGSEVVKCTSNRSTMRTKTLMKMKTRSRLMDPPTPTYPDMVSGR 210

Query: 102 -----------SSQKLETAVSEEGKNQD--------ETEDMPEEYNQLKLSPFSVLQWVI 161
                      S +  +     +G ++D          ED+PE   + K+  + +++W+ 
Sbjct: 211 TPRSGNLNPGFSGRNTKPGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIF 270

Query: 162 FVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLISTWAVGLIVKIIERNFL 221
            +++I  LIC+  +P L+ K++WDL LWKWE+ VL LICGRL+S+W V L V  +E NFL
Sbjct: 271 LILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFL 330

Query: 222 LRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRSKILPYITKILICFLVGA 281
            RK+VLYFVYG+R+ V+NCLWL L+L+ WHF+F++KV+R  RS +L Y+TK+LIC LV  
Sbjct: 331 WRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAV 390

Query: 282 SIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSPLFERSNTEDMVKDNQNA 341
            IWL+KTL VK+LAS FH++ YF+R++E+LF+QYVI TLSG P  E    E+ V ++   
Sbjct: 391 IIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIHIEEEKVANDVKT 450

Query: 342 ---DGREESPKSGRIIC--------SGRLVNCDGSRQLKPESSRQ-------DEEIPVDQ 401
               GR+ SP   + +         SGRL          P  SR        +E I +D 
Sbjct: 451 FEIVGRKLSPLGPKAVSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDH 510

Query: 402 LNKLNQHNISAWNMRRMMNIVRNGALLTLDEKILSETKEE--YLLQIRSERQAKEAAGNI 461
           L ++N  N+SAW M+++MN+++ G L TLDE+I   T +E     QIRSE +AK AA  I
Sbjct: 511 LQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKI 570

Query: 462 FRRVSRRGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQRKV 521
           F+ V+  GS +I ++D MRF++++E+   M LF   +E   I +   + W+VNAF +R+ 
Sbjct: 571 FQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRA 630

Query: 522 LVLSLNDANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIFGNT 581
           L L+LND  TAV+ LH ++++ V++ IL+IWL+ILG     FL+ ISSQLLL+VF+FGN+
Sbjct: 631 LALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNS 690

Query: 582 CKTVFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVLATK 641
           CKT+FEA+IF+FVMHPFDVGDRCE+DGVQM+VEEM ILTTVFLR+DNQKI YPNS+L TK
Sbjct: 691 CKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTK 750

Query: 642 AIGNYYRSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDVEDL 699
            I NYYRSPDM + ++F+VHI+TP +K + ++++I  Y+++K + WHP+P++  +D+  L
Sbjct: 751 PIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGL 810

BLAST of MC11g0077 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 604.0 bits (1556), Expect = 1.6e-172
Identity = 336/707 (47.52%), Postives = 463/707 (65.49%), Query Frame = 0

Query: 35  AQTMRRRGKE-----SSDSENRRPSSASGGGEVLRCSSNASFRGCSWSSPISKKSRLMDP 94
           A  +RRR        SSD E R    A    EVL+C S          S    KSRL DP
Sbjct: 151 ADAVRRRQNRTSLGGSSDEEGRNRDEA----EVLKCGSKKPM-----LSRNKTKSRLQDP 210

Query: 95  P----------EEDSSQKLETAVS-----------------EEGKNQDE--TEDMPEEYN 154
           P          E  S ++     S                 EE + +D    ED+PEE+ 
Sbjct: 211 PTPTHPAIDKTEMKSGRRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFK 270

Query: 155 QLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLISTW 214
           + KLS +  L+W+  V+++  L+C+  +  L+RK+ W L LWKWE+TVL LICGRL+S+W
Sbjct: 271 RDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSW 330

Query: 215 AVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRSKIL 274
            V +IV ++E+NF  RKRVLYFVYG+R++V+NCLWL L+LL WHF+F++KV+R TRS  L
Sbjct: 331 IVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTAL 390

Query: 275 PYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSPLFE 334
            Y+T++L+C LV   IWL+KT+ VK+LAS FH++ YF+R++E+LF+QYVI TLSG PL E
Sbjct: 391 RYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLME 450

Query: 335 RSNTEDM-------VKDNQNADGREESPKSGRIICSGRLV-NCDGSRQLKPESSRQDEEI 394
               E+        VK  +   G +  P     + S   V    G  ++  +     E I
Sbjct: 451 IQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGI 510

Query: 395 PVDQLNKLNQHNISAWNMRRMMNIVRNGALLTLDEKILSETKE-EYLLQIRSERQAKEAA 454
            +DQL ++N  N+SAWNM+R+MNI+  GA+ TLD+ +   T+E E    IRSE +AK AA
Sbjct: 511 RIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSEYEAKCAA 570

Query: 455 GNIFRRVSRRGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQ 514
             IF  V+  GS +I L+D +RF+ +EEA   M+LF   +E+  I +   + W+V AF +
Sbjct: 571 RKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRE 630

Query: 515 RKVLVLSLNDANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIF 574
           R+ L L+LND  TAVD LH ++N+ + + I++IWL+ILG     FL+ +SSQLLL+ F+F
Sbjct: 631 RRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVF 690

Query: 575 GNTCKTVFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVL 634
           GN+CKT+FEAIIFLFVMHPFDVGDRCE+DGVQ+VVEEM ILTTVFLRYDNQKI YPNSVL
Sbjct: 691 GNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVL 750

Query: 635 ATKAIGNYYRSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDV 694
            TK I NYYRSPDMG+ V+F VHI+TP +K++ IK++I  Y+++K + W+PAP++    +
Sbjct: 751 GTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSM 810

Query: 695 EDLEKVKMSVCVTHKMNHQDMGERWNRRAALVEEIVKIFRQLDDEHQ 699
           +DL  VK++V +TH+MNHQDMGER+ RR  L+EE+ K  R+LD E++
Sbjct: 811 DDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYR 848

BLAST of MC11g0077 vs. TAIR 10
Match: AT2G17000.1 (Mechanosensitive ion channel family protein )

HSP 1 Score: 563.5 bits (1451), Expect = 2.4e-160
Identity = 322/721 (44.66%), Postives = 477/721 (66.16%), Query Frame = 0

Query: 7   VLLKIDSFGNGADKESEEKNFQCLPERSAQTMRRRGKESSDSENRRPSSASGGGEVLRCS 66
           V  KI+S G   +     ++       S+ TMR   ++  + E         G  V+RCS
Sbjct: 109 VFFKINSSGTN-NMSGSVRSCTSSTSFSSATMRLNLEQQLEDE---------GEVVVRCS 168

Query: 67  SNASFRGCSWSSPISKKSRLMDPPEED---------SSQKLETAV----SEEGKNQDET- 126
              S R     S    +SRL+DPP+E+         +S +L + +    S++ + +D++ 
Sbjct: 169 ---SVRKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSS 228

Query: 127 --EDMPEEYNQLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLA 186
             ED+P EY +LK+   ++LQW+  + ++V L+ +  +   +  ++W L LWKWE+ +L 
Sbjct: 229 AEEDVPVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLV 288

Query: 187 LICGRLISTWAVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEK 246
           LICGRL+S   + +IV  IERNFLLRKRVLYFVYG++ AV+NCLWL L+LL WHF+F++K
Sbjct: 289 LICGRLVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKK 348

Query: 247 VQRTTRSKILPYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVI 306
           V++ T+S +L  ++KIL+CFL+   +WL+KTL VK+LAS FHV+ YF+R++EALF  Y+I
Sbjct: 349 VEKETQSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLI 408

Query: 307 VTLSGSPLFERSNTEDMVKDNQNADGREESPKSGRIIC------------SGRLVNCDGS 366
            TLSG P+ E S  E+  ++++  D   +  K G  +             SG  +N   S
Sbjct: 409 ETLSGPPMLELSRIEE--EEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTMNMKFS 468

Query: 367 RQLKPESSRQDEEIPVDQLNKLNQHNISAWNMRRMMNIVRNGALLTLDEKILSET-KEEY 426
             +    S  D  I +D L+K+NQ N+SAWNM+R+M IVRN +L TLDE+ L  T ++E 
Sbjct: 469 PIIPKTGS--DNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCEDES 528

Query: 427 LLQIRSERQAKEAAGNIFRRVSRRGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCID 486
             QIRSE++AK AA  IF+ V++ G++ I L+D+MRF+  +EA+ TM LF     T+ I 
Sbjct: 529 TRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTKKIT 588

Query: 487 EFSFREWMVNAFNQRKVLVLSLNDANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFL 546
           + + + W+VNAF +R+ L L+LND  TAV+ LH++++   A+ I+VIWLI+L      +L
Sbjct: 589 KSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATSKYL 648

Query: 547 IFISSQLLLLVFIFGNTCKTVFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFL 606
           +F++SQ++LL F+FGN+ KTVFE+IIFLF++HP+DVGDR  +D V+MVVEEM ILTTVFL
Sbjct: 649 LFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTTVFL 708

Query: 607 RYDNQKITYPNSVLATKAIGNYYRSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKS 666
           R DN KI YPN +L  KAI NY RSPDMG+ V   VHI+TP +K++ IK++I+ YI+SK 
Sbjct: 709 RADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYIDSKP 768

Query: 667 EEWHPAPVVTMKDVEDLEKVKMSVCVTHKMNHQDMGERWNRRAALVEEIVKIFRQLDDEH 699
           E W+P   V +KDVEDL  V++++ + HK+NHQ+MGER+ RRA L+EE++KI  +LD ++
Sbjct: 769 EYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELDIQY 812

BLAST of MC11g0077 vs. TAIR 10
Match: AT3G14810.2 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 542.7 bits (1397), Expect = 4.3e-154
Identity = 317/707 (44.84%), Postives = 435/707 (61.53%), Query Frame = 0

Query: 35  AQTMRRRGKE-----SSDSENRRPSSASGGGEVLRCSSNASFRGCSWSSPISKKSRLMDP 94
           A  +RRR        SSD E R    A    EVL+C S          S    KSRL DP
Sbjct: 151 ADAVRRRQNRTSLGGSSDEEGRNRDEA----EVLKCGSKKPM-----LSRNKTKSRLQDP 210

Query: 95  P----------EEDSSQKLETAVS-----------------EEGKNQDE--TEDMPEEYN 154
           P          E  S ++     S                 EE + +D    ED+PEE+ 
Sbjct: 211 PTPTHPAIDKTEMKSGRRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFK 270

Query: 155 QLKLSPFSVLQWVIFVIVIVGLICNRWVPILKRKSIWDLPLWKWELTVLALICGRLISTW 214
           + KLS +  L+W+  V+++  L+C+  +  L+RK+ W L LWKWE+TVL LICGRL+S+W
Sbjct: 271 RDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSW 330

Query: 215 AVGLIVKIIERNFLLRKRVLYFVYGLRRAVRNCLWLSLLLLVWHFIFNEKVQRTTRSKIL 274
            V +IV ++E+NF  RKRVLYFVYG+R++V+NCLWL L+LL WHF+F++KV+R TRS  L
Sbjct: 331 IVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTAL 390

Query: 275 PYITKILICFLVGASIWLLKTLAVKILASFFHVNNYFERLKEALFSQYVIVTLSGSPLFE 334
                                              YF+R++E+LF+QYVI TLSG PL E
Sbjct: 391 ---------------------------------RTYFDRIQESLFTQYVIETLSGPPLME 450

Query: 335 RSNTEDM-------VKDNQNADGREESPKSGRIICSGRLV-NCDGSRQLKPESSRQDEEI 394
               E+        VK  +   G +  P     + S   V    G  ++  +     E I
Sbjct: 451 IQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGI 510

Query: 395 PVDQLNKLNQHNISAWNMRRMMNIVRNGALLTLDEKILSETKE-EYLLQIRSERQAKEAA 454
            +DQL ++N  N+SAWNM+R+MNI+  GA+ TLD+ +   T+E E    IRSE +AK AA
Sbjct: 511 RIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATHIRSEYEAKCAA 570

Query: 455 GNIFRRVSRRGSEFINLDDVMRFMNKEEALMTMSLFGPDAETQCIDEFSFREWMVNAFNQ 514
             IF  V+  GS +I L+D +RF+ +EEA   M+LF   +E+  I +   + W+  AF +
Sbjct: 571 RKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWV--AFRE 630

Query: 515 RKVLVLSLNDANTAVDGLHNLLNIFVAVTILVIWLIILGTPILHFLIFISSQLLLLVFIF 574
           R+ L L+LND  TAVD LH ++N+ + + I++IWL+ILG     FL+ +SSQLLL+ F+F
Sbjct: 631 RRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVF 690

Query: 575 GNTCKTVFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMRILTTVFLRYDNQKITYPNSVL 634
           GN+CKT+FEAIIFLFVMHPFDVGDRCE+DGVQ+VVEEM ILTTVFLRYDNQKI YPNSVL
Sbjct: 691 GNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVL 750

Query: 635 ATKAIGNYYRSPDMGEGVDFYVHISTPSKKVSLIKEQITRYIESKSEEWHPAPVVTMKDV 694
            TK I NYYRSPDMG+ V+F VHI+TP +K++ IK++I  Y+++K + W+PAP++    +
Sbjct: 751 GTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSM 810

Query: 695 EDLEKVKMSVCVTHKMNHQDMGERWNRRAALVEEIVKIFRQLDDEHQ 699
           +DL  VK++V +TH+MNHQDMGER+ RR  L+EE+ K  R+LD E++
Sbjct: 811 DDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYR 813