MC11g0069 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g0069 vs. ExPASy Swiss-Prot
Match: Q3MIT2 (tRNA pseudouridine synthase Pus10 OS=Homo sapiens OX=9606 GN=PUS10 PE=1 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 7.9e-62
Identity = 169/479 (35.28%), Postives = 256/479 (53.44%), Query Frame = 0

Query: 57  PCIIRLSGIERHNYSLS------VSASILGSDKEAINDDNNRNTCRLCLGILQFIYIDDK 116
           P  IRL  +E    +LS      +S S +GS     + ++N N C +CLGILQ     D 
Sbjct: 68  PKKIRLQELEDSIDNLSQNGEGRISVSHVGS---TASKNSNLNVCNVCLGILQEFCEKD- 127

Query: 117 GNFVKCKGADDLAVSISEMVKKEGHQIDSFSLEVSVPSIMLENEKLVWLYLKRKYGSEHW 176
             F+K          + + V+  G +  S    VS P  +   E   WL +K++ G +  
Sbjct: 128 --FIK---------KVCQKVEASGFEFTSLVFSVSFPPQLSVREHAAWLLVKQEMGKQSL 187

Query: 177 FQGKPPPLCLSAKDALKICITKPL--ETLLDCKSSSSGLRIRLTYSHPK--------ASS 236
             G+     +  K+A K  IT PL  E L       S   + + ++HP+        A+ 
Sbjct: 188 SLGRDD--IVQLKEAYK-WITHPLFSEELGVPIDGKSLFEVSVVFAHPETVEDCHFLAAI 247

Query: 237 C--------NDNSIKRSQGGCK-MRKIAGNDEPSDISNCGANSVADDCFNPSKEVELPKC 296
           C        N  S+       K + KI   D       C  NS    C     E+E    
Sbjct: 248 CPDCFKPAKNKQSVFTRMAVMKALNKIKEEDFLKQFP-CPPNSPKAVC--AVLEIECAHG 307

Query: 297 FEF---PQEKYSRNVSQTCWVIGDERMGEASVEEILGNNILPLCQGDSYKFHAAGREDLD 356
             F      KYSRN+ QT W+I  ER  E+SVEE++ +++L + + +S+ F ++GRED+D
Sbjct: 308 AVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVD 367

Query: 357 VRMLGSGRPFLVEIQNARHIP-SEMIINEMESKINSSENKLVGVRNLKVVGSEGWAFVQE 416
           VR LG+GRPF +E+ N   +  +   I E++ KIN+S NK + VR+L++V  E    ++E
Sbjct: 368 VRTLGNGRPFAIELVNPHRVHFTSQEIKELQQKINNSSNK-IQVRDLQLVTREAIGHMKE 427

Query: 417 GEAEKQKQYAALVWTSRPLEDDDLLSISSLKDLKVLQRTPIRVLHRRSPLEREKIIHWMK 476
           GE EK K Y+AL+WT++ ++  D+  ++ +KDLK+ Q+TP+RVLHRR    R ++IH+M+
Sbjct: 428 GEEEKTKTYSALIWTNKAIQKKDIEFLNDIKDLKIDQKTPLRVLHRRPLAVRARVIHFME 487

Query: 477 IEKFAESSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSFGSILSCRAEILQLDVTDIKMD 507
            +   E   ++ LHL TQAGTYIKEFVHGD GRT P+ GS+++  A+IL+LDV  + +D
Sbjct: 488 TQYVDE--HHFRLHLKTQAGTYIKEFVHGDFGRTKPNIGSLMNVTADILELDVESVDVD 522

BLAST of MC11g0069 vs. ExPASy Swiss-Prot
Match: Q9D3U0 (tRNA pseudouridine synthase Pus10 OS=Mus musculus OX=10090 GN=Pus10 PE=1 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 6.3e-59
Identity = 161/448 (35.94%), Postives = 234/448 (52.23%), Query Frame = 0

Query: 82  DKEAINDDNNRNTCRLCLGILQFIYIDDKGNFVKCKGADDLAVSISEMVKKEGHQIDSFS 141
           D+      +    C +CLGILQ     +KG   K          + + V+  G +  S  
Sbjct: 94  DESMAEKPSKLRVCNVCLGILQ--EFCEKGFITK----------VCQKVEASGFEFTSVV 153

Query: 142 LEVSVPSIMLENEKLVWLYLKRKYGSEHWFQGKPPPLCLSAKDALKICITKPL--ETLLD 201
           L VS P  +   E   WL +K++ G +    G+   + L  K+A K  IT PL  E L  
Sbjct: 154 LSVSFPPQLSVREHAAWLLVKQEMGKQSLSLGRNDVVQL--KEAYK-WITHPLFSEELGV 213

Query: 202 CKSSSSGLRIRLTYSHPK-ASSC---------------NDNSIKRSQGGCK-MRKIAGND 261
                S   + + ++HP+ A  C               N  S+       K + KI   D
Sbjct: 214 PTDGKSLFEVSVVFAHPETAEDCHFLGEVCRDCFKPAKNKQSVFTRMAVLKALSKIKEED 273

Query: 262 EPSDISNCGANSVADDCFNPSKEVELPKCFEF---PQEKYSRNVSQTCWVIGDERMGEAS 321
                  C  NS    C     EVE      F      KYSRN+ QT W+I  ER  E+S
Sbjct: 274 FLGQFP-CPPNSPKTVC--TVLEVECTHGAVFVAGRYNKYSRNLPQTPWIIDGERKMESS 333

Query: 322 VEEILGNNILPLCQGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARHIP-SEMIINEME 381
           VEE++ +++L + + +S+ F ++GRED+DVR LG+GRPF VE+ N   +  +   + E++
Sbjct: 334 VEELISDHLLAVFRAESFNFSSSGREDVDVRTLGNGRPFAVELLNPHRVHFTSQEMKELQ 393

Query: 382 SKINSSENKLVGVRNLKVVGSEGWAFVQEGEAEKQKQYAALVWTSRPLEDDDLLSISSLK 441
             IN S +K + VR+L++V  E    ++EGE EK K Y+AL+WT+R ++  D+  +  LK
Sbjct: 394 QTINKSSDK-IQVRDLQLVTREAIGHMKEGEEEKTKTYSALIWTNRAIQKKDIGFLDDLK 453

Query: 442 DLKVLQRTPIRVLHRRSPLEREKIIHWMKIEKFAESSQYYLLHLCTQAGTYIKEFVHGDL 501
           DLK+ Q+TP+RVLHRR    R + IH MK     E   ++ LHL TQAGTYIKEFVHGD 
Sbjct: 454 DLKIDQKTPLRVLHRRPLAVRTRAIHSMKTHYLDE--HHFRLHLKTQAGTYIKEFVHGDF 513

Query: 502 GRTHPSFGSILSCRAEILQLDVTDIKMD 507
           GRT P+ GS+++  A+IL+LDV  + +D
Sbjct: 514 GRTKPNLGSLMNVTADILELDVESVDVD 520

BLAST of MC11g0069 vs. ExPASy Swiss-Prot
Match: Q6ING2 (tRNA pseudouridine synthase Pus10 OS=Xenopus laevis OX=8355 GN=pus10 PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 9.3e-55
Identity = 152/450 (33.78%), Postives = 230/450 (51.11%), Query Frame = 0

Query: 84  EAINDDNNRNTCRLCLGILQFIYIDDKGNFVKCKGADDLAVSISEMVKKEGHQIDSFSLE 143
           E +N       C LCLGILQ         F + +  + + V I+  V    +++  F L 
Sbjct: 85  EDVNGSQVVRICPLCLGILQ--------QFCEPEFIEKVFVKINSAV----YELKDFVLS 144

Query: 144 VSVPSIMLENEKLVWLYLKRKYGSEHWFQGKPPPLCLSAKDALKICITKPLETLLDCKSS 203
           +S+P+ +   E   WL  K++ G       K     +  K+A K  I   L   L  ++ 
Sbjct: 145 ISLPAQLSVREHSAWLQAKQEMGKHGHSLDKTD--IVQLKEAYKWIIHPLLSDQLGIQAD 204

Query: 204 SSGL-RIRLTYSHPK--------ASSCND--------NSIKRSQGGCK-MRKIAGND--- 263
           S  L  + + ++HP+        A+ C D         S+       K + KI   D   
Sbjct: 205 SKSLFEVAVVFTHPETDGDCHFLATVCRDCFKPTKNKQSVFTRMAVVKALEKIKEEDFRV 264

Query: 264 -----EPSDISNCGANSVADDCFNPSKEVELPKCFEFPQEKYSRNVSQTCWVIGDERMGE 323
                  S  + C    V D   N S     P        KYSRN+ QT W+I  ER  E
Sbjct: 265 QFPFPPSSPETTC---EVVDIQCNHS-----PVFVAGRYNKYSRNLPQTPWIIDGERKIE 324

Query: 324 ASVEEILGNNILPLCQGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARHIP-SEMIINE 383
           +SVEE++ ++++   +  S+ F ++GRED+DVR LG GRPF +E+ N   +  +   I  
Sbjct: 325 SSVEELITDHLVAAFRSSSFNFSSSGREDVDVRTLGKGRPFAIELLNPHKVQFTGQEIKA 384

Query: 384 MESKINSSENKLVGVRNLKVVGSEGWAFVQEGEAEKQKQYAALVWTSRPLEDDDLLSISS 443
           ++ KIN+S+   + VR+L++V  E  A ++EGE EK K Y AL+W  + + ++DL  +  
Sbjct: 385 LQQKINTSDK--IKVRDLQIVSREAVAHMKEGEEEKTKCYCALIWIEKMVRNEDLQLLDG 444

Query: 444 LKDLKVLQRTPIRVLHRRSPLEREKIIHWMKIEKFAESSQYYLLHLCTQAGTYIKEFVHG 503
           L++L + Q+TP+RVLHRR    R + IH M+ E   E   ++ L+L TQAGTYIKEFVHG
Sbjct: 445 LEELTIAQKTPLRVLHRRPLASRSRTIHTMRTEYVDE--HHFRLYLKTQAGTYIKEFVHG 504

Query: 504 DLGRTHPSFGSILSCRAEILQLDVTDIKMD 507
           D GRT P+ GSI+   A+IL+LDV  + +D
Sbjct: 505 DFGRTKPNVGSIMETNADILELDVESVDVD 508

BLAST of MC11g0069 vs. ExPASy Swiss-Prot
Match: Q9VPK7 (Putative tRNA pseudouridine synthase Pus10 OS=Drosophila melanogaster OX=7227 GN=Pus10 PE=2 SV=2)

HSP 1 Score: 144.1 bits (362), Expect = 4.5e-33
Identity = 138/492 (28.05%), Postives = 227/492 (46.14%), Query Frame = 0

Query: 49  LLSIGVCVPCIIR---LSGIERHNYS-----LSVSASILGSDKEAINDDNNRNTCRLCLG 108
           L S GVC  C +R     G E  N +     L V  +    ++E +  + ++ + R  LG
Sbjct: 10  LRSCGVCEICQLRYLKARGAEYRNINETLQKLDVKLNENVENEELVISEGSQPSKRARLG 69

Query: 109 ILQFIYIDDKGNFVKCKGADDLAVSI--SEMVKKEGHQIDSFSLEVSVPSIMLENEKLVW 168
           +         G F K    ++L  SI  S+  K +  +I    L + +P  +   +  +W
Sbjct: 70  VCSTCL----GLFSK-DFQNELLSSILASDFAKYDCQKI---VLAICLPMSLQLRQLAMW 129

Query: 169 LYLKRKYGSEHWFQGKPPPLCLSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSH--- 228
             L+R++G     +  PP + +  K+A+K+ +   +   L     ++GL I +  +H   
Sbjct: 130 FALRRRFGPS-IDENNPPDVPI--KEAVKLILHPIICEKLAKVYDANGLMINIDLTHSLE 189

Query: 229 ----PKASSCNDNSIKRSQGGCKMRKIA-----GNDEPSDI-SNCGANSVADDCFNPSKE 288
                K    N  +        K  +I+        +PS + +          C N  + 
Sbjct: 190 GEEVEKLVKLNKEAFPAKASHQKRIEISRGLLEKQYQPSKVKAETYEKYFQIPCSNVEES 249

Query: 289 VELPK--------CFEFPQEKYSRNVSQTCWVIGDERMGEASVEEILGNNILPLCQGDSY 348
           ++L          C      K SR +S T W++  +R+ E S+EEI+  ++ P       
Sbjct: 250 IKLTSIDLQGPLICVAGRYRKLSRELSHTPWILNGQRLMEDSIEEIIIRHVGPHFTEKLG 309

Query: 349 K--FHAAGREDLDVRMLGSGRPFLVEIQNA-RHIPSEMIINEMESKINSSENKLVGVRNL 408
           K  F ++GRED+DVR LG GRPF++EI NA R   ++     ME  +++S   +V + +L
Sbjct: 310 KITFMSSGREDVDVRCLGKGRPFVLEIPNAKRSCLTKDEAFAMERAVDAS--GMVSIHHL 369

Query: 409 KVVGSEGWAFVQEGEAEKQKQYAALVWTSRPLEDDDLLSISSLKDLKVLQRTPIRVLHRR 468
           +VV  E    ++ GE +K+K Y AL     P+    L  +       + Q+TPIRVLHRR
Sbjct: 370 QVVPREELTHIKTGEEQKKKFYRALCALQEPVSVKILEQLQISASFNIQQKTPIRVLHRR 429

Query: 469 SPLEREKIIHWMKIEKFAESSQYYLLHLCTQAGTYIKEFVHGDLGRTHPSFGSILSCRAE 507
               R + I+ +  +    + +  ++ + TQAGTYIKE VHG+ GRT PS  SI+    +
Sbjct: 430 PLHTRPRTIYSVTAKVHRGNPKALIIDIVTQAGTYIKELVHGEFGRTTPSLSSIIGKPMD 488

BLAST of MC11g0069 vs. ExPASy Swiss-Prot
Match: A1RX33 (tRNA pseudouridine synthase Pus10 OS=Thermofilum pendens (strain DSM 2475 / Hrk 5) OX=368408 GN=pus10 PE=3 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 4.5e-33
Identity = 86/229 (37.55%), Postives = 128/229 (55.90%), Query Frame = 0

Query: 278 KYSRNVSQTCWVIGDERMG-EASVEEILGNNILPLCQGDSYKFHAAGREDLDVRMLGSGR 337
           KY+R + Q  W   DER+    S+EE++    L L + +  KFHAAGRED+DVR LG+GR
Sbjct: 216 KYARGLPQNPWPQPDERIKFNTSIEELIVKPALELFEAEKAKFHAAGREDIDVRTLGTGR 275

Query: 338 PFLVEIQNARHIPSEMIINEMESKINSSENKLVGVRNLKVVGSEGWAFVQEGEAEKQKQY 397
           PF++EI+  R     + +  +  KINS    L+ V +L     +    ++   +  +K Y
Sbjct: 276 PFVLEIKKPR--KRNIDLKVLAEKINSGAGGLIEVLDLAYTDRKTIKKLKSLASIAKKAY 335

Query: 398 AALVWTSRPLEDDDLLSISSLKDLKVL-QRTPIRVLHRRSPLEREKIIHWMKIEKFAESS 457
            A V   +P++D+ L  IS +    V+ QRTP RVLHRR    R+KI++ ++  K ++  
Sbjct: 336 VARVKFEKPVDDEKLAEISKVFSNAVINQRTPTRVLHRRVDKLRKKIVYRLEARKISQDE 395

Query: 458 QYYLLHLCTQAGTYIKEFVHGDLGRTHPSFGSILSCRAEILQLDVTDIK 505
             + L   TQ G Y+KEF+HGD GRT PS    L      ++LDV  I+
Sbjct: 396 VEFYLE--TQGGFYVKEFIHGDNGRTTPSIAEFLGNNVLSIELDVVSIE 440

BLAST of MC11g0069 vs. NCBI nr
Match: XP_022141855.1 (putative tRNA pseudouridine synthase Pus10 [Momordica charantia])

HSP 1 Score: 1009 bits (2609), Expect = 0.0
Identity = 511/535 (95.51%), Postives = 511/535 (95.51%), Query Frame = 0

Query: 1   MAGQRNEVQSDSEALLLTSAPEEAISQNEQLQLLDDVVPQIPSHVMKDLLSIGVCVPCII 60
           MAGQRNEVQSDSEALLLTSAPEEAISQNEQLQLLDDVVPQIPSHVMKDLLSIGVCVPCII
Sbjct: 1   MAGQRNEVQSDSEALLLTSAPEEAISQNEQLQLLDDVVPQIPSHVMKDLLSIGVCVPCII 60

Query: 61  RLSGIERHNYSLSVSASILGSDKEAINDDNNRNTCRLCLGILQFIYIDDKGNFVKCKGAD 120
           RLSGIERHNYSLSVSASILGSDKEAINDDNNRNTCRLCLGILQFIYIDDKGNFVKCKGAD
Sbjct: 61  RLSGIERHNYSLSVSASILGSDKEAINDDNNRNTCRLCLGILQFIYIDDKGNFVKCKGAD 120

Query: 121 DLAVSISEMVKKEGHQIDSFSLEVSVPSIMLENEKLVWLYLKRKYGSEHWFQGKPPPLCL 180
           DLAVSISEMVKKEGHQIDSFSLEVSVPSIMLENEKLVWLYLKRKYGSEHWFQGKPPPLCL
Sbjct: 121 DLAVSISEMVKKEGHQIDSFSLEVSVPSIMLENEKLVWLYLKRKYGSEHWFQGKPPPLCL 180

Query: 181 SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASSCNDNSIKRSQGGCKMRKIAG 240
           SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASSCNDNSIKRSQGGCKMRKIAG
Sbjct: 181 SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASSCNDNSIKRSQGGCKMRKIAG 240

Query: 241 NDEPSDISNCGANSVADDCFNPSKEVELPKCFEFPQEK---------------------- 300
           NDEPSDISNCGANSVADDCFNPSKEVELPKCFEFPQEK                      
Sbjct: 241 NDEPSDISNCGANSVADDCFNPSKEVELPKCFEFPQEKVNEPCHLALICYRAPIYLVGRY 300

Query: 301 --YSRNVSQTCWVIGDERMGEASVEEILGNNILPLCQGDSYKFHAAGREDLDVRMLGSGR 360
             YSRNVSQTCWVIGDERMGEASVEEILGNNILPLCQGDSYKFHAAGREDLDVRMLGSGR
Sbjct: 301 IKYSRNVSQTCWVIGDERMGEASVEEILGNNILPLCQGDSYKFHAAGREDLDVRMLGSGR 360

Query: 361 PFLVEIQNARHIPSEMIINEMESKINSSENKLVGVRNLKVVGSEGWAFVQEGEAEKQKQY 420
           PFLVEIQNARHIPSEMIINEMESKINSSENKLVGVRNLKVVGSEGWAFVQEGEAEKQKQY
Sbjct: 361 PFLVEIQNARHIPSEMIINEMESKINSSENKLVGVRNLKVVGSEGWAFVQEGEAEKQKQY 420

Query: 421 AALVWTSRPLEDDDLLSISSLKDLKVLQRTPIRVLHRRSPLEREKIIHWMKIEKFAESSQ 480
           AALVWTSRPLEDDDLLSISSLKDLKVLQRTPIRVLHRRSPLEREKIIHWMKIEKFAESSQ
Sbjct: 421 AALVWTSRPLEDDDLLSISSLKDLKVLQRTPIRVLHRRSPLEREKIIHWMKIEKFAESSQ 480

Query: 481 YYLLHLCTQAGTYIKEFVHGDLGRTHPSFGSILSCRAEILQLDVTDIKMDSHFTE 511
           YYLLHLCTQAGTYIKEFVHGDLGRTHPSFGSILSCRAEILQLDVTDIKMDSHFTE
Sbjct: 481 YYLLHLCTQAGTYIKEFVHGDLGRTHPSFGSILSCRAEILQLDVTDIKMDSHFTE 535

BLAST of MC11g0069 vs. NCBI nr
Match: XP_022937403.1 (putative tRNA pseudouridine synthase Pus10 isoform X1 [Cucurbita moschata] >XP_022937404.1 putative tRNA pseudouridine synthase Pus10 isoform X2 [Cucurbita moschata])

HSP 1 Score: 872 bits (2254), Expect = 0.0
Identity = 440/535 (82.24%), Postives = 474/535 (88.60%), Query Frame = 0

Query: 1   MAGQRNEVQSDSEALLLTSAPEEAISQNEQLQLLDDVVPQIPSHVMKDLLSIGVCVPCII 60
           MA ++ EV +D EA +L+SA ++AI  N++L +L+D VPQ+PS+V KDLLSIGVC+PCII
Sbjct: 1   MANEQEEVAADLEATVLSSAAQQAIFPNDELHVLNDAVPQMPSYVTKDLLSIGVCIPCII 60

Query: 61  RLSGIERHNYSLSVSASILGSDKEAINDDNNRNTCRLCLGILQFIYIDDKGNFVKCKGAD 120
           RLSGIERH+YS S+S  I+GSDKEAI+DD +RN C LCLGILQF YIDDKGNFVKCKG D
Sbjct: 61  RLSGIERHHYSFSLSVPIVGSDKEAIDDDIDRNICCLCLGILQFSYIDDKGNFVKCKGVD 120

Query: 121 DLAVSISEMVKKEGHQIDSFSLEVSVPSIMLENEKLVWLYLKRKYGSEHWFQGKPPPLCL 180
           DLAVSISEMVKKEGHQIDSFSLEVS+PSI+LENEKLVWLYLKRKYGSEHWFQGKPPP+ L
Sbjct: 121 DLAVSISEMVKKEGHQIDSFSLEVSIPSIILENEKLVWLYLKRKYGSEHWFQGKPPPISL 180

Query: 181 SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASSCNDNSIKRSQGGCKMRKIAG 240
           SAKDALKIC+TKPLETLLDCKSSSSGLRIRLTYSHPKAS+ ND+S KR Q GCKMRKIAG
Sbjct: 181 SAKDALKICVTKPLETLLDCKSSSSGLRIRLTYSHPKASNSNDSSNKRHQDGCKMRKIAG 240

Query: 241 NDEPSDISNCGANSVADDCFNPSKEVELPKCFEFPQEK---------------------- 300
           NDE  DIS  GANSVAD+CFNPS+EVEL KCFEFPQEK                      
Sbjct: 241 NDETLDISKFGANSVADNCFNPSQEVELSKCFEFPQEKINEPCHLALICYRTSIYLIGRY 300

Query: 301 --YSRNVSQTCWVIGDERMGEASVEEILGNNILPLCQGDSYKFHAAGREDLDVRMLGSGR 360
             YSRNVSQT WVIGDERMGEASVEEILGNNILPLC+GDSYKFHAAGREDLDVRMLGSGR
Sbjct: 301 IKYSRNVSQTRWVIGDERMGEASVEEILGNNILPLCRGDSYKFHAAGREDLDVRMLGSGR 360

Query: 361 PFLVEIQNARHIPSEMIINEMESKINSSENKLVGVRNLKVVGSEGWAFVQEGEAEKQKQY 420
           PFLVEIQNAR +PSEMIINE+ESKIN S+NKL+GVRNLKVVGSEGWAFVQEGEAEKQKQY
Sbjct: 361 PFLVEIQNARLLPSEMIINEIESKINGSKNKLIGVRNLKVVGSEGWAFVQEGEAEKQKQY 420

Query: 421 AALVWTSRPLEDDDLLSISSLKDLKVLQRTPIRVLHRRSPLEREKIIHWMKIEKFAESSQ 480
           AALVWTSRPLEDDDLLSISSLKDLK+LQRTPIRVLHRRSPLEREKIIHWM+IEK AESSQ
Sbjct: 421 AALVWTSRPLEDDDLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMRIEKVAESSQ 480

Query: 481 YYLLHLCTQAGTYIKEFVHGDLGRTHPSFGSILSCRAEILQLDVTDIKMDSHFTE 511
           YYLLHLCTQAGTYIKEFVHGDLGRTHPS GSILSCRAEILQLDVTDIKMD H  E
Sbjct: 481 YYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDGHLAE 535

BLAST of MC11g0069 vs. NCBI nr
Match: XP_023535551.1 (putative tRNA pseudouridine synthase Pus10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 868 bits (2244), Expect = 4.31e-315
Identity = 439/535 (82.06%), Postives = 474/535 (88.60%), Query Frame = 0

Query: 1   MAGQRNEVQSDSEALLLTSAPEEAISQNEQLQLLDDVVPQIPSHVMKDLLSIGVCVPCII 60
           MA +++EV +D EA LL+SA ++AIS N++L +L+D VPQ+PS+V KDLLSIGVC+PCII
Sbjct: 1   MANEQDEVAADLEATLLSSAAQQAISPNDELHVLNDAVPQMPSYVTKDLLSIGVCIPCII 60

Query: 61  RLSGIERHNYSLSVSASILGSDKEAINDDNNRNTCRLCLGILQFIYIDDKGNFVKCKGAD 120
           RLSGIERH+YS S+S  I+GSDKEAI+DD +RN C LCLGILQF YIDDKGNFVKCKG D
Sbjct: 61  RLSGIERHHYSFSLSVPIVGSDKEAIDDDIDRNICCLCLGILQFSYIDDKGNFVKCKGVD 120

Query: 121 DLAVSISEMVKKEGHQIDSFSLEVSVPSIMLENEKLVWLYLKRKYGSEHWFQGKPPPLCL 180
           DLAVSISE+VKKEGHQID+FSLEVS+PSI+LENEKLVWLYLKRKYGSEHWFQGKPPP+ L
Sbjct: 121 DLAVSISELVKKEGHQIDNFSLEVSIPSIILENEKLVWLYLKRKYGSEHWFQGKPPPISL 180

Query: 181 SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASSCNDNSIKRSQGGCKMRKIAG 240
           SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKAS+ N +S KR Q GCKMRKIA 
Sbjct: 181 SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGSSNKRQQDGCKMRKIAC 240

Query: 241 NDEPSDISNCGANSVADDCFNPSKEVELPKCFEFPQEK---------------------- 300
           NDE  DIS  GANSVAD+CFNPS+EVEL KCFEFPQEK                      
Sbjct: 241 NDETLDISKYGANSVADNCFNPSQEVELSKCFEFPQEKINEPCHLALICYRTSIYLIGRY 300

Query: 301 --YSRNVSQTCWVIGDERMGEASVEEILGNNILPLCQGDSYKFHAAGREDLDVRMLGSGR 360
             YSRNVSQT WVIGDERMGEASVEEILGNNILPLC+GDSYKFHAAGREDLDVRMLGSGR
Sbjct: 301 IKYSRNVSQTRWVIGDERMGEASVEEILGNNILPLCRGDSYKFHAAGREDLDVRMLGSGR 360

Query: 361 PFLVEIQNARHIPSEMIINEMESKINSSENKLVGVRNLKVVGSEGWAFVQEGEAEKQKQY 420
           PFLVEIQNAR +PSEMIINE+ESKIN S+NKL+GVRNLKVVGSEGWAFVQEGEAEKQKQY
Sbjct: 361 PFLVEIQNARLLPSEMIINEIESKINGSKNKLIGVRNLKVVGSEGWAFVQEGEAEKQKQY 420

Query: 421 AALVWTSRPLEDDDLLSISSLKDLKVLQRTPIRVLHRRSPLEREKIIHWMKIEKFAESSQ 480
           AALVWTSRPLEDDDLLSISSLKDLK+LQRTPIRVLHRRSPLEREKIIHWM+IEK AESSQ
Sbjct: 421 AALVWTSRPLEDDDLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMRIEKVAESSQ 480

Query: 481 YYLLHLCTQAGTYIKEFVHGDLGRTHPSFGSILSCRAEILQLDVTDIKMDSHFTE 511
           YYLLHLCTQAGTYIKEFVHGDLGRTHPS GSILSCRAEILQLDVTDIKMD H  E
Sbjct: 481 YYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDGHLAE 535

BLAST of MC11g0069 vs. NCBI nr
Match: XP_022975902.1 (putative tRNA pseudouridine synthase Pus10 [Cucurbita maxima])

HSP 1 Score: 865 bits (2236), Expect = 7.03e-314
Identity = 437/535 (81.68%), Postives = 472/535 (88.22%), Query Frame = 0

Query: 1   MAGQRNEVQSDSEALLLTSAPEEAISQNEQLQLLDDVVPQIPSHVMKDLLSIGVCVPCII 60
           MA +++EV +D EA LLTSA ++AIS N++L +L+D VPQ+PS+V KDL SIGVC+PCII
Sbjct: 1   MANEQDEVAADLEATLLTSAAQQAISPNDELHVLNDAVPQMPSYVTKDLSSIGVCIPCII 60

Query: 61  RLSGIERHNYSLSVSASILGSDKEAINDDNNRNTCRLCLGILQFIYIDDKGNFVKCKGAD 120
           RLSGIERH+YS S+S  I+GSDK+AI+DD +RN C LCLGILQF YIDDKGNFVKCKG D
Sbjct: 61  RLSGIERHHYSFSLSVPIVGSDKKAIDDDIDRNICCLCLGILQFSYIDDKGNFVKCKGVD 120

Query: 121 DLAVSISEMVKKEGHQIDSFSLEVSVPSIMLENEKLVWLYLKRKYGSEHWFQGKPPPLCL 180
           DLAVSISEMVKKEGHQIDSFSLEVS+PSI+LENEKLVWLYLKRKYGSEHWFQGKPPP+ L
Sbjct: 121 DLAVSISEMVKKEGHQIDSFSLEVSIPSIILENEKLVWLYLKRKYGSEHWFQGKPPPISL 180

Query: 181 SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASSCNDNSIKRSQGGCKMRKIAG 240
           SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKAS+ N +S KR Q  CKMRKIAG
Sbjct: 181 SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGSSNKRHQDSCKMRKIAG 240

Query: 241 NDEPSDISNCGANSVADDCFNPSKEVELPKCFEFPQEK---------------------- 300
           NDE  DIS   ANSVAD+CFNPS+EVEL +CFEFPQEK                      
Sbjct: 241 NDETLDISKFSANSVADNCFNPSQEVELSRCFEFPQEKINEPCHLALICYRTSIYLIGRY 300

Query: 301 --YSRNVSQTCWVIGDERMGEASVEEILGNNILPLCQGDSYKFHAAGREDLDVRMLGSGR 360
             YSRNVSQT WVIGDERMGEASVEEILGNNILPLC+GDSYKFHAAGREDLDVRMLGSGR
Sbjct: 301 IKYSRNVSQTRWVIGDERMGEASVEEILGNNILPLCRGDSYKFHAAGREDLDVRMLGSGR 360

Query: 361 PFLVEIQNARHIPSEMIINEMESKINSSENKLVGVRNLKVVGSEGWAFVQEGEAEKQKQY 420
           PFLVEIQNAR +PSEMI+NE+ESKIN S+NKL+GVRNLKVVGSEGWAFVQEGEAEKQKQY
Sbjct: 361 PFLVEIQNARLLPSEMIVNEIESKINGSKNKLIGVRNLKVVGSEGWAFVQEGEAEKQKQY 420

Query: 421 AALVWTSRPLEDDDLLSISSLKDLKVLQRTPIRVLHRRSPLEREKIIHWMKIEKFAESSQ 480
           AALVWTSRPLEDDDLLSISSLKDLK+LQRTPIRVLHRRSPLEREKIIHWM+IEK AESSQ
Sbjct: 421 AALVWTSRPLEDDDLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMRIEKVAESSQ 480

Query: 481 YYLLHLCTQAGTYIKEFVHGDLGRTHPSFGSILSCRAEILQLDVTDIKMDSHFTE 511
           YYLLHLCTQAGTYIKEFVHGDLGRTHPS GSILSCRAEILQLDVTDIKMD H  E
Sbjct: 481 YYLLHLCTQAGTYIKEFVHGDLGRTHPSMGSILSCRAEILQLDVTDIKMDGHLAE 535

BLAST of MC11g0069 vs. NCBI nr
Match: XP_038897427.1 (tRNA pseudouridine synthase Pus10 [Benincasa hispida])

HSP 1 Score: 865 bits (2234), Expect = 1.13e-313
Identity = 438/529 (82.80%), Postives = 470/529 (88.85%), Query Frame = 0

Query: 1   MAGQRNEVQSDSEALLLTSAPEEAISQNEQLQLLDDVVPQIPSHVMKDLLSIGVCVPCII 60
           MA +++ VQ+D EA  LTSA +EAIS N++ QLL+  +PQ+PSH   DLLSIGVC+PCII
Sbjct: 1   MANEQDVVQADPEATPLTSATQEAISPNDEPQLLNHALPQMPSHATIDLLSIGVCIPCII 60

Query: 61  RLSGIERHNYSLSVSASILGSDKEAINDDNNRNTCRLCLGILQFIYIDDKGNFVKCKGAD 120
           RLSGIERHNYS S+S  ILGSDKEAI+ DN+R+TC LCLG+LQF YIDDKGN++K KGAD
Sbjct: 61  RLSGIERHNYSFSLSVPILGSDKEAIDADNDRSTCSLCLGVLQFSYIDDKGNYIKFKGAD 120

Query: 121 DLAVSISEMVKKEGHQIDSFSLEVSVPSIMLENEKLVWLYLKRKYGSEHWFQGKPPPLCL 180
           DLAVSISEMVKKEGHQIDSFSLEVSVPSI+LENEK VWLYLKRKYGSE WFQGKPPP+CL
Sbjct: 121 DLAVSISEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPICL 180

Query: 181 SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASSCNDNSIKRSQGGCKMRKIAG 240
           SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKAS  N NSIKR+Q GCKMRKI G
Sbjct: 181 SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASKSNGNSIKRNQNGCKMRKITG 240

Query: 241 NDEPSDISNCGANSVADDCFNPSKEVELPK------------CFEFP------QEKYSRN 300
           NDEPSDISNC ANSVADDCFNPS+EVE P+            C+           KYSRN
Sbjct: 241 NDEPSDISNCDANSVADDCFNPSQEVEFPQETIKEPCHLALICYRTSIYLVGRYIKYSRN 300

Query: 301 VSQTCWVIGDERMGEASVEEILGNNILPLCQGDSYKFHAAGREDLDVRMLGSGRPFLVEI 360
           VSQT WVIG+ERMGEASVEEI+GNNILPLC+GD+YKFHAAGREDLDVRMLGSGRPFLVEI
Sbjct: 301 VSQTRWVIGEERMGEASVEEIIGNNILPLCRGDNYKFHAAGREDLDVRMLGSGRPFLVEI 360

Query: 361 QNARHIPSEMIINEMESKINSSENKLVGVRNLKVVGSEGWAFVQEGEAEKQKQYAALVWT 420
           QNAR +PSEMIINE++SKINSSENKLVGVRNLKVVGSEGWA VQEGEAEKQKQYAALVWT
Sbjct: 361 QNARLLPSEMIINEIQSKINSSENKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALVWT 420

Query: 421 SRPLEDDDLLSISSLKDLKVLQRTPIRVLHRRSPLEREKIIHWMKIEKFAESSQYYLLHL 480
           SRPLEDDDLLSISSLKDLK+LQRTPIRVLHRRSPLEREKIIHWMKIEK AESSQYYLLHL
Sbjct: 421 SRPLEDDDLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLLHL 480

Query: 481 CTQAGTYIKEFVHGDLGRTHPSFGSILSCRAEILQLDVTDIKMDSHFTE 511
           CTQAGTYIKEFVHGDLGRTHPS GSILSCRAEILQLDVTDIKMD HF E
Sbjct: 481 CTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDYHFAE 529

BLAST of MC11g0069 vs. ExPASy TrEMBL
Match: A0A6J1CJA2 (tRNA pseudouridine(55) synthase OS=Momordica charantia OX=3673 GN=LOC111012116 PE=3 SV=1)

HSP 1 Score: 1009 bits (2609), Expect = 0.0
Identity = 511/535 (95.51%), Postives = 511/535 (95.51%), Query Frame = 0

Query: 1   MAGQRNEVQSDSEALLLTSAPEEAISQNEQLQLLDDVVPQIPSHVMKDLLSIGVCVPCII 60
           MAGQRNEVQSDSEALLLTSAPEEAISQNEQLQLLDDVVPQIPSHVMKDLLSIGVCVPCII
Sbjct: 1   MAGQRNEVQSDSEALLLTSAPEEAISQNEQLQLLDDVVPQIPSHVMKDLLSIGVCVPCII 60

Query: 61  RLSGIERHNYSLSVSASILGSDKEAINDDNNRNTCRLCLGILQFIYIDDKGNFVKCKGAD 120
           RLSGIERHNYSLSVSASILGSDKEAINDDNNRNTCRLCLGILQFIYIDDKGNFVKCKGAD
Sbjct: 61  RLSGIERHNYSLSVSASILGSDKEAINDDNNRNTCRLCLGILQFIYIDDKGNFVKCKGAD 120

Query: 121 DLAVSISEMVKKEGHQIDSFSLEVSVPSIMLENEKLVWLYLKRKYGSEHWFQGKPPPLCL 180
           DLAVSISEMVKKEGHQIDSFSLEVSVPSIMLENEKLVWLYLKRKYGSEHWFQGKPPPLCL
Sbjct: 121 DLAVSISEMVKKEGHQIDSFSLEVSVPSIMLENEKLVWLYLKRKYGSEHWFQGKPPPLCL 180

Query: 181 SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASSCNDNSIKRSQGGCKMRKIAG 240
           SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASSCNDNSIKRSQGGCKMRKIAG
Sbjct: 181 SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASSCNDNSIKRSQGGCKMRKIAG 240

Query: 241 NDEPSDISNCGANSVADDCFNPSKEVELPKCFEFPQEK---------------------- 300
           NDEPSDISNCGANSVADDCFNPSKEVELPKCFEFPQEK                      
Sbjct: 241 NDEPSDISNCGANSVADDCFNPSKEVELPKCFEFPQEKVNEPCHLALICYRAPIYLVGRY 300

Query: 301 --YSRNVSQTCWVIGDERMGEASVEEILGNNILPLCQGDSYKFHAAGREDLDVRMLGSGR 360
             YSRNVSQTCWVIGDERMGEASVEEILGNNILPLCQGDSYKFHAAGREDLDVRMLGSGR
Sbjct: 301 IKYSRNVSQTCWVIGDERMGEASVEEILGNNILPLCQGDSYKFHAAGREDLDVRMLGSGR 360

Query: 361 PFLVEIQNARHIPSEMIINEMESKINSSENKLVGVRNLKVVGSEGWAFVQEGEAEKQKQY 420
           PFLVEIQNARHIPSEMIINEMESKINSSENKLVGVRNLKVVGSEGWAFVQEGEAEKQKQY
Sbjct: 361 PFLVEIQNARHIPSEMIINEMESKINSSENKLVGVRNLKVVGSEGWAFVQEGEAEKQKQY 420

Query: 421 AALVWTSRPLEDDDLLSISSLKDLKVLQRTPIRVLHRRSPLEREKIIHWMKIEKFAESSQ 480
           AALVWTSRPLEDDDLLSISSLKDLKVLQRTPIRVLHRRSPLEREKIIHWMKIEKFAESSQ
Sbjct: 421 AALVWTSRPLEDDDLLSISSLKDLKVLQRTPIRVLHRRSPLEREKIIHWMKIEKFAESSQ 480

Query: 481 YYLLHLCTQAGTYIKEFVHGDLGRTHPSFGSILSCRAEILQLDVTDIKMDSHFTE 511
           YYLLHLCTQAGTYIKEFVHGDLGRTHPSFGSILSCRAEILQLDVTDIKMDSHFTE
Sbjct: 481 YYLLHLCTQAGTYIKEFVHGDLGRTHPSFGSILSCRAEILQLDVTDIKMDSHFTE 535

BLAST of MC11g0069 vs. ExPASy TrEMBL
Match: A0A6J1FB31 (tRNA pseudouridine(55) synthase OS=Cucurbita moschata OX=3662 GN=LOC111443704 PE=3 SV=1)

HSP 1 Score: 872 bits (2254), Expect = 0.0
Identity = 440/535 (82.24%), Postives = 474/535 (88.60%), Query Frame = 0

Query: 1   MAGQRNEVQSDSEALLLTSAPEEAISQNEQLQLLDDVVPQIPSHVMKDLLSIGVCVPCII 60
           MA ++ EV +D EA +L+SA ++AI  N++L +L+D VPQ+PS+V KDLLSIGVC+PCII
Sbjct: 1   MANEQEEVAADLEATVLSSAAQQAIFPNDELHVLNDAVPQMPSYVTKDLLSIGVCIPCII 60

Query: 61  RLSGIERHNYSLSVSASILGSDKEAINDDNNRNTCRLCLGILQFIYIDDKGNFVKCKGAD 120
           RLSGIERH+YS S+S  I+GSDKEAI+DD +RN C LCLGILQF YIDDKGNFVKCKG D
Sbjct: 61  RLSGIERHHYSFSLSVPIVGSDKEAIDDDIDRNICCLCLGILQFSYIDDKGNFVKCKGVD 120

Query: 121 DLAVSISEMVKKEGHQIDSFSLEVSVPSIMLENEKLVWLYLKRKYGSEHWFQGKPPPLCL 180
           DLAVSISEMVKKEGHQIDSFSLEVS+PSI+LENEKLVWLYLKRKYGSEHWFQGKPPP+ L
Sbjct: 121 DLAVSISEMVKKEGHQIDSFSLEVSIPSIILENEKLVWLYLKRKYGSEHWFQGKPPPISL 180

Query: 181 SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASSCNDNSIKRSQGGCKMRKIAG 240
           SAKDALKIC+TKPLETLLDCKSSSSGLRIRLTYSHPKAS+ ND+S KR Q GCKMRKIAG
Sbjct: 181 SAKDALKICVTKPLETLLDCKSSSSGLRIRLTYSHPKASNSNDSSNKRHQDGCKMRKIAG 240

Query: 241 NDEPSDISNCGANSVADDCFNPSKEVELPKCFEFPQEK---------------------- 300
           NDE  DIS  GANSVAD+CFNPS+EVEL KCFEFPQEK                      
Sbjct: 241 NDETLDISKFGANSVADNCFNPSQEVELSKCFEFPQEKINEPCHLALICYRTSIYLIGRY 300

Query: 301 --YSRNVSQTCWVIGDERMGEASVEEILGNNILPLCQGDSYKFHAAGREDLDVRMLGSGR 360
             YSRNVSQT WVIGDERMGEASVEEILGNNILPLC+GDSYKFHAAGREDLDVRMLGSGR
Sbjct: 301 IKYSRNVSQTRWVIGDERMGEASVEEILGNNILPLCRGDSYKFHAAGREDLDVRMLGSGR 360

Query: 361 PFLVEIQNARHIPSEMIINEMESKINSSENKLVGVRNLKVVGSEGWAFVQEGEAEKQKQY 420
           PFLVEIQNAR +PSEMIINE+ESKIN S+NKL+GVRNLKVVGSEGWAFVQEGEAEKQKQY
Sbjct: 361 PFLVEIQNARLLPSEMIINEIESKINGSKNKLIGVRNLKVVGSEGWAFVQEGEAEKQKQY 420

Query: 421 AALVWTSRPLEDDDLLSISSLKDLKVLQRTPIRVLHRRSPLEREKIIHWMKIEKFAESSQ 480
           AALVWTSRPLEDDDLLSISSLKDLK+LQRTPIRVLHRRSPLEREKIIHWM+IEK AESSQ
Sbjct: 421 AALVWTSRPLEDDDLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMRIEKVAESSQ 480

Query: 481 YYLLHLCTQAGTYIKEFVHGDLGRTHPSFGSILSCRAEILQLDVTDIKMDSHFTE 511
           YYLLHLCTQAGTYIKEFVHGDLGRTHPS GSILSCRAEILQLDVTDIKMD H  E
Sbjct: 481 YYLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDGHLAE 535

BLAST of MC11g0069 vs. ExPASy TrEMBL
Match: A0A6J1IEB1 (tRNA pseudouridine(55) synthase OS=Cucurbita maxima OX=3661 GN=LOC111476467 PE=3 SV=1)

HSP 1 Score: 865 bits (2236), Expect = 3.41e-314
Identity = 437/535 (81.68%), Postives = 472/535 (88.22%), Query Frame = 0

Query: 1   MAGQRNEVQSDSEALLLTSAPEEAISQNEQLQLLDDVVPQIPSHVMKDLLSIGVCVPCII 60
           MA +++EV +D EA LLTSA ++AIS N++L +L+D VPQ+PS+V KDL SIGVC+PCII
Sbjct: 1   MANEQDEVAADLEATLLTSAAQQAISPNDELHVLNDAVPQMPSYVTKDLSSIGVCIPCII 60

Query: 61  RLSGIERHNYSLSVSASILGSDKEAINDDNNRNTCRLCLGILQFIYIDDKGNFVKCKGAD 120
           RLSGIERH+YS S+S  I+GSDK+AI+DD +RN C LCLGILQF YIDDKGNFVKCKG D
Sbjct: 61  RLSGIERHHYSFSLSVPIVGSDKKAIDDDIDRNICCLCLGILQFSYIDDKGNFVKCKGVD 120

Query: 121 DLAVSISEMVKKEGHQIDSFSLEVSVPSIMLENEKLVWLYLKRKYGSEHWFQGKPPPLCL 180
           DLAVSISEMVKKEGHQIDSFSLEVS+PSI+LENEKLVWLYLKRKYGSEHWFQGKPPP+ L
Sbjct: 121 DLAVSISEMVKKEGHQIDSFSLEVSIPSIILENEKLVWLYLKRKYGSEHWFQGKPPPISL 180

Query: 181 SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASSCNDNSIKRSQGGCKMRKIAG 240
           SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKAS+ N +S KR Q  CKMRKIAG
Sbjct: 181 SAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGSSNKRHQDSCKMRKIAG 240

Query: 241 NDEPSDISNCGANSVADDCFNPSKEVELPKCFEFPQEK---------------------- 300
           NDE  DIS   ANSVAD+CFNPS+EVEL +CFEFPQEK                      
Sbjct: 241 NDETLDISKFSANSVADNCFNPSQEVELSRCFEFPQEKINEPCHLALICYRTSIYLIGRY 300

Query: 301 --YSRNVSQTCWVIGDERMGEASVEEILGNNILPLCQGDSYKFHAAGREDLDVRMLGSGR 360
             YSRNVSQT WVIGDERMGEASVEEILGNNILPLC+GDSYKFHAAGREDLDVRMLGSGR
Sbjct: 301 IKYSRNVSQTRWVIGDERMGEASVEEILGNNILPLCRGDSYKFHAAGREDLDVRMLGSGR 360

Query: 361 PFLVEIQNARHIPSEMIINEMESKINSSENKLVGVRNLKVVGSEGWAFVQEGEAEKQKQY 420
           PFLVEIQNAR +PSEMI+NE+ESKIN S+NKL+GVRNLKVVGSEGWAFVQEGEAEKQKQY
Sbjct: 361 PFLVEIQNARLLPSEMIVNEIESKINGSKNKLIGVRNLKVVGSEGWAFVQEGEAEKQKQY 420

Query: 421 AALVWTSRPLEDDDLLSISSLKDLKVLQRTPIRVLHRRSPLEREKIIHWMKIEKFAESSQ 480
           AALVWTSRPLEDDDLLSISSLKDLK+LQRTPIRVLHRRSPLEREKIIHWM+IEK AESSQ
Sbjct: 421 AALVWTSRPLEDDDLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMRIEKVAESSQ 480

Query: 481 YYLLHLCTQAGTYIKEFVHGDLGRTHPSFGSILSCRAEILQLDVTDIKMDSHFTE 511
           YYLLHLCTQAGTYIKEFVHGDLGRTHPS GSILSCRAEILQLDVTDIKMD H  E
Sbjct: 481 YYLLHLCTQAGTYIKEFVHGDLGRTHPSMGSILSCRAEILQLDVTDIKMDGHLAE 535

BLAST of MC11g0069 vs. ExPASy TrEMBL
Match: A0A1S3BUF4 (tRNA pseudouridine(55) synthase OS=Cucumis melo OX=3656 GN=LOC103493791 PE=3 SV=1)

HSP 1 Score: 830 bits (2144), Expect = 2.22e-300
Identity = 428/531 (80.60%), Postives = 457/531 (86.06%), Query Frame = 0

Query: 1   MAGQRNEVQSDSEA--LLLTSAPEEAISQNEQLQLLDDVVPQIPSHVMKDLLSIGVCVPC 60
           MA +++EVQ++ EA  L LTS  +EAIS  ++LQ         PS+  KDLLSIGVC+PC
Sbjct: 1   MANEQDEVQANPEATPLALTSVTQEAISPGDKLQF--------PSYAAKDLLSIGVCIPC 60

Query: 61  IIRLSGIERHNYSLSVSASILGSDKEAINDDNNRNTCRLCLGILQFIYIDDKGNFVKCKG 120
           IIRLSGIERHNYS S+SA ILGSD EAI DDN+RNTC LCLGILQF YIDDKG+F+KCKG
Sbjct: 61  IIRLSGIERHNYSFSLSAPILGSDTEAIEDDNDRNTCCLCLGILQFSYIDDKGDFIKCKG 120

Query: 121 ADDLAVSISEMVKKEGHQIDSFSLEVSVPSIMLENEKLVWLYLKRKYGSEHWFQGKPPPL 180
            DDLAVSISEMVKKEGHQIDSFSLEVSVPSI+LENEK VWLYLKRKYGSE WFQGKPPP+
Sbjct: 121 VDDLAVSISEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPI 180

Query: 181 CLSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASSCNDNSIKRSQGGCKMRKI 240
           CLSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKAS+ N NSIKR+Q G KMRKI
Sbjct: 181 CLSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASNGNGNSIKRNQDGFKMRKI 240

Query: 241 AGNDEPSDISNCGANSVADDCFNPSKEVELPK------------CFEFP------QEKYS 300
            G+DEPSDISNC  NSVADDC NPS+EV  P+            C+           KYS
Sbjct: 241 IGSDEPSDISNCNVNSVADDCINPSQEVIFPQETIKEPCHLALTCYRTSIYMFGRYIKYS 300

Query: 301 RNVSQTCWVIGDERMGEASVEEILGNNILPLCQGDSYKFHAAGREDLDVRMLGSGRPFLV 360
           RNVSQT WVIG+ERMGEASVEEILGNNILP C+GDSYKFHAAGREDLDVRMLGSGRPFLV
Sbjct: 301 RNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLV 360

Query: 361 EIQNARHIPSEMIINEMESKINSSENKLVGVRNLKVVGSEGWAFVQEGEAEKQKQYAALV 420
           EIQNAR +PSEMIINE++SKINS E KLVGVRNLKVVGSEGWA VQEGEAEKQKQYAALV
Sbjct: 361 EIQNARLLPSEMIINEIQSKINSPETKLVGVRNLKVVGSEGWALVQEGEAEKQKQYAALV 420

Query: 421 WTSRPLEDDDLLSISSLKDLKVLQRTPIRVLHRRSPLEREKIIHWMKIEKFAESSQYYLL 480
           WTSRPLEDDD LSISSLKDLK+LQRTPIRVLHRRSPLEREKIIHWMKIEK A+SSQYYLL
Sbjct: 421 WTSRPLEDDDFLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAKSSQYYLL 480

Query: 481 HLCTQAGTYIKEFVHGDLGRTHPSFGSILSCRAEILQLDVTDIKMDSHFTE 511
           HLCTQAGTYIKEFVHGDLGRTHPS GSILSCRAEILQLDVTDIKMD H  E
Sbjct: 481 HLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLAE 523

BLAST of MC11g0069 vs. ExPASy TrEMBL
Match: A0A0A0L378 (tRNA pseudouridine(55) synthase OS=Cucumis sativus OX=3659 GN=Csa_4G653400 PE=3 SV=1)

HSP 1 Score: 827 bits (2135), Expect = 4.83e-299
Identity = 429/531 (80.79%), Postives = 460/531 (86.63%), Query Frame = 0

Query: 1   MAGQRNEVQSDSEA--LLLTSAPEEAISQNEQLQLLDDVVPQIPSHVMKDLLSIGVCVPC 60
           MA +++EVQ++ EA  L LTS  +EAIS  ++LQ        IPS+  KDLLSIGVC+PC
Sbjct: 1   MANEQDEVQANPEATALALTSVTQEAISPGDKLQ--------IPSYATKDLLSIGVCIPC 60

Query: 61  IIRLSGIERHNYSLSVSASILGSDKEAINDDNNRNTCRLCLGILQFIYIDDKGNFVKCKG 120
           IIRLSGIERH+YS S+SA ILGSD EAI+D N+RNTC LCLGILQF YIDDKG+F+KCKG
Sbjct: 61  IIRLSGIERHSYSFSLSAPILGSDNEAIDDANDRNTCCLCLGILQFSYIDDKGDFIKCKG 120

Query: 121 ADDLAVSISEMVKKEGHQIDSFSLEVSVPSIMLENEKLVWLYLKRKYGSEHWFQGKPPPL 180
            DDLAVSISEMVKKEGHQIDSFSLEVSVPSI+LENEK VWLYLKRKYGSE WFQGKPPP+
Sbjct: 121 VDDLAVSISEMVKKEGHQIDSFSLEVSVPSIILENEKRVWLYLKRKYGSEQWFQGKPPPI 180

Query: 181 CLSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASSCNDNSIKRSQGGCKMRKI 240
           CLSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKAS+ N NSIKR+Q G KMRKI
Sbjct: 181 CLSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASNSNGNSIKRNQDGFKMRKI 240

Query: 241 AGNDEPSDISNCGANSVADDCFNPSKEVELPK------------CFEFP------QEKYS 300
            G+DEPSDISNC  NSVADDC NPS+EV  P+            C+           KYS
Sbjct: 241 VGSDEPSDISNC--NSVADDCINPSQEVVFPQETIKEPCHLALTCYRTSIYIVGRYIKYS 300

Query: 301 RNVSQTCWVIGDERMGEASVEEILGNNILPLCQGDSYKFHAAGREDLDVRMLGSGRPFLV 360
           RNVSQT WVIG+ERMGEASVEEILGNNILP C+GDSYKFHAAGREDLDVRMLGSGRPFLV
Sbjct: 301 RNVSQTRWVIGEERMGEASVEEILGNNILPFCRGDSYKFHAAGREDLDVRMLGSGRPFLV 360

Query: 361 EIQNARHIPSEMIINEMESKINSSENKLVGVRNLKVVGSEGWAFVQEGEAEKQKQYAALV 420
           EIQNAR +PSEMIINE++SKINSSENKL+GVRNLKVVGSEGWA VQEGEAEKQKQYAALV
Sbjct: 361 EIQNARLLPSEMIINEIQSKINSSENKLIGVRNLKVVGSEGWALVQEGEAEKQKQYAALV 420

Query: 421 WTSRPLEDDDLLSISSLKDLKVLQRTPIRVLHRRSPLEREKIIHWMKIEKFAESSQYYLL 480
           WTSRPLED DLLSISSLKDLK+LQRTPIRVLHRRSPLEREKIIHWMKIEK AESSQYYLL
Sbjct: 421 WTSRPLEDADLLSISSLKDLKILQRTPIRVLHRRSPLEREKIIHWMKIEKVAESSQYYLL 480

Query: 481 HLCTQAGTYIKEFVHGDLGRTHPSFGSILSCRAEILQLDVTDIKMDSHFTE 511
           HLCTQAGTYIKEFVHGDLGRTHPS GSILSCRAEILQLDVTDIKMD H  E
Sbjct: 481 HLCTQAGTYIKEFVHGDLGRTHPSIGSILSCRAEILQLDVTDIKMDCHLGE 521

BLAST of MC11g0069 vs. TAIR 10
Match: AT1G20410.1 (Pseudouridine synthase family protein )

HSP 1 Score: 491.9 bits (1265), Expect = 6.3e-139
Identity = 268/513 (52.24%), Postives = 340/513 (66.28%), Query Frame = 0

Query: 1   MAGQRNEVQSDSEALLLTSAPEEAISQ-NEQLQLLDDVVPQIPSHVMKDLLSIGVCVPCI 60
           M+G++  V  +S  L     P   +S  N + + + +    + +  +KDLLS+GVC  CI
Sbjct: 1   MSGEKEPVAVESAGL-----PNGLVSHGNSKTRRVYETARSLHTGAVKDLLSLGVCERCI 60

Query: 61  IRLSGIERHNYSL-SVSASIL------GSDKEAINDDNNRNTCRLCLGILQFIYIDDKGN 120
            RL  +E  +  + SVS S L      G D+ + ++ +    C +CLGILQF++ D K  
Sbjct: 61  FRLVSVEAFDSDISSVSTSTLRSWLKSGDDETSASESSCSTICIVCLGILQFVFSDVKKE 120

Query: 121 FVKCKGADDLAVSISEMVKKEGHQIDSFSLEVSVPSIMLENEKLVWLYLKRKYGSEHWFQ 180
            VK   + D    I+++VK++ H+ DSF LEVSVPS ++ENE+ +  YLK KY +E W Q
Sbjct: 121 LVKSDSSSDYVARITDLVKQDRHEFDSFGLEVSVPSTIMENERALLSYLKGKYSTEVWLQ 180

Query: 181 GKPPPLCLSAKDALKICITKPLETLLDCKSSSSGLRIRLTYSHPKASSCNDNSIKRSQGG 240
                  +S KDALK+ +   L+  L  +S SS   IRLTY+  KAS         +   
Sbjct: 181 RDK----ISVKDALKVLLLDQLKASLGAESDSSSFHIRLTYT--KASD-EAQGASETTHE 240

Query: 241 CKMRKIAGNDEPSDISNCGANSVADDCFNPSKEVELPKCFEFPQEKYSRNVSQTCWVIGD 300
            K RK    +  + IS      V + C        +P  F     KYSRNVSQ+ W+I D
Sbjct: 241 SKRRKTDAENGSNCISENSFEKVYEPCIFSVHCNRMPIFFSGRYFKYSRNVSQSRWIIDD 300

Query: 301 ERMGEASVEEILGNNILPLCQGDSYKFHAAGREDLDVRMLGSGRPFLVEIQNARHIPSEM 360
           ERMGEASVEEILG NILP C GDSYKFHAAGRED+DVRMLGSGRPFL+E+QN+R +PSE 
Sbjct: 301 ERMGEASVEEILGGNILPACLGDSYKFHAAGREDIDVRMLGSGRPFLIEVQNSRQLPSEQ 360

Query: 361 IINEMESKINSSENKLVGVRNLKVVGSEGWAFVQEGEAEKQKQYAALVWTSRPLEDDDLL 420
            + E+E KIN SE KLVGV++LK +GS+ WA ++EGEAEKQKQY ALVW SRPLE+ D  
Sbjct: 361 SLKEVEEKINKSEKKLVGVKDLKCIGSQCWAMMREGEAEKQKQYVALVWISRPLEEKDFN 420

Query: 421 SISSLKDLKVLQRTPIRVLHRRSPLEREKIIHWMKIEKFAESSQYYLLHLCTQAGTYIKE 480
           SIS LK+LK+LQ+TP+RVLHRRSPL+REKIIHWMK+EK    S Y+LLHLCTQAGTYIKE
Sbjct: 421 SISCLKELKILQKTPVRVLHRRSPLDREKIIHWMKVEKIKGHSHYFLLHLCTQAGTYIKE 480

Query: 481 FVHGDLGRTHPSFGSILSCRAEILQLDVTDIKM 506
           FVHGDLGRT PS GSIL CRAEI+QLDVTD+KM
Sbjct: 481 FVHGDLGRTTPSMGSILGCRAEIIQLDVTDVKM 501