MC11g0065 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g0065
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	4-coumarate--CoA ligase
Location	MC11: 451915 .. 455833 (+)
RNA-Seq Expression	MC11g0065
Synteny	MC11g0065

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCGCAGACATTTATTTCCACACGTTCGGAATTCAATTTCCAGCATAATTTTCTCGTTAAAAAATTGCGAGCCGGAAATCGGAAGTCTAATAGTGGCAAATCCATACACGCTTTTTTTTAAAAAAATAATATAAACGAAATTATTATTTATTTTCAGAATTAAAAAACAGGTTGACCAGTCAACGACGGCGGCTCTGCACCTCCTATACATGCGAACGCGAGGACCTGCACGGCCACAAACAGTCTCCACCTTCTACTCTCACTTCTCTCTCGGACGGCGCCGGCGATTCACCCTTCTCCACCTACTTTTCCGACAGTCAAATGGCGCTCTACGGCGGCGGCGACAATCCCGCAGTGGATCCAAGAAGCGGCTTCTGCAGCTCCACTAAAATCTTCCACAGCAAACGCCGCCCAATCCCACTCCCACCCAAGCAATCTCTGAATGCGACGTCGTTTATCTCTTCCCGCCCCCACAACGGCAAAATCGCACTCATCGACGCCGCCACCGGCCAACATATCACCTACTCCCAACTCTGGGAATTCGTCGATTCCGTCGCCGCCGCTCTCTCCGACATCGGCATCAGAAAAGGCCACGTCATCCTCCTTCTATCCCCAAATTCAATTTTCTTCCCCATCATCTGCCTCGCAGTCATGTCCATCGGCGCAATCATCACCACAACCAACCCACTAAACACCGCCCAAGAAATCGCCAAACAAATCGCCGATTCGAAGCCGATTTTGGCCTTCACAACCCAGCAACTGATCCCCAAAATCGCCAGTTCGAATTTGCCCATAGTCCTAATCGACGGTCAAAATGAAAACCCCGCAGGAAATAAAGTCAAAATCGTGTCGAATTTGGGGGAAATGATGAGGAAAAATCCAAGCGGGAGCAGAATTACGGAGCCGGTGGAGCAGAACGACACAGCGACTCTGCTCTATTCCTCTGGAACGACAGGGGCGAGTAAAGGCGTGGTGTCGTCCCACAAGAATATCATAGCCATGGTTCAAGTCGTCGTGACGCGATTCAATTTAAGCGACGGGGAAGGAACTTTCATCTGTACAGTTCCGATGTTTCATATTTATGGCCTCGTGGCGTTCGCGACGGGGCTTCTCTCGTCGGGATCGACGATCGTTGTGTTGTCGAAATTCGAGATTCACGAAATGCTGTCGGCGATCGAGAGGTACCGGGCGACGTTTCTTCCCCTCGTGCCGCCGATTCTGGTAGCGCTCGTGAACGCCGCCGATCAGATCAAGGGGAAGTACGATTTGAGTTCGCTGCGTACGGCACTTTCCGGTGGAGCGCCGCTGGGGAAGGAGGTGATAGAGGGGTTTGTGGAGAAGTATCCGAATGTTACGATTCTTCAGGGCTACGGCTTAACGGAATCGACCGGAATTGGGGCGTCCACCGATTCTCTGGAGGAGAGCCGGCGGTACGGCACGGCGGGGCTTCTGTCGCCGAGCACGGAGGGGAAGATCGTGGACCCTGATAGCGGCGAAGCGCTGCCGGTGAAGCGGACGGGGGAGCTTTGGCTGAGAGGGCCCACCATAATGAAAGGTAAAAAATGGCAAATGGCCGACATGATTTTACCCAATTTGACACGTGGTCCTTGTTCGGTACTGCAGTATTTGATATTTGTTTCGTTCAAATAAATCCAGAAAAGAAAAGTTAAATTAAGTCTTTAATAATTGGAGCAATGTAACGGCAAATTACGGATTTAGACAAAAAGGGCGGAAAAGTGATTGTGGCTTTGGTTGTTTTGTTGCAGGGTATTTTGGTAACGTGGAGGCCACGACCTCAACACTTGACTCGGAGGGATGGTTGAGAACTGGAGATCTGTGTTACATTGATGAAGATGGTTTTATTTTTGTGGTTGATAGGCTTAAGGAACTCATCAAATATAAGGGTTATCAGGTAATTTTGATTCATATTGTTTTTAATTTTCATTTTTTTACCATCGTAGATATATATATATATATATATTGAAAACTATTTATTAAAATATAATAATCTTTCTCTCTCTTGTATTGCATTTTTTCTCTTCATGCTTACAAAAAAATTAATGAGGGTCCAGAGGAATCTCCAATTGGCTATAGATAGCTAGCTACATACAATAAAAGTTTGTTTTATTTTAATGAGAAAAATTATAGAGAACATGCTCGTTCGCATATATATATATATATATATATATATTAATGTACATTTGTTTATTTTGTTTGTTTAATGTTACTTTCAGGTCCCTCCTGCTGAATTAGAGGCTTTATTACTTACTCATCCTAATATCTCTGATGCAGCTGTGATTCCGTAAGTTCAATTTTTCCATGGATCTTAGTTTTTTACTTCAATTCAACCAAATGCTTATAAAAATTTTAGTTAATTCTATAAAATGTTTGTTACTTAATTAATTTTTTGTTTTACACATGCCAGAAGAAAAGTTTTATAAACCATTTGAATTGTTTAATTCAAGGGAGTTAAATAATAACAAGAAAGAATCAAGAGCCACCTTCAAACTTCAAGACTCTTTCCTTTTTTAATAATTTGTATTTGTATTTTTTTCTCTTTCGAAAAATTAAATCTAAGGCAATGGCCCCACATGATTGGGATCTTTTGAATAATAAAGTTGAGTAGGTTGGGATAATATGTTTTATTATACCATGGATTGTTTATTCAAGGAGCTAAACAGACACAGCAATAGAATATTCAAAAAGTATATAATTTGTGATATATATATATATATATATATATAAATATGAGTGATGGTTCGAATCTTCGAACTTTTGTTCGAGAATATATGTGTGAGCGTTGAGCTGCTCAAATTATAATTGTAGTTGTTTTGTTCTCTTTTTTCTAATATAATGTAATTTTAAGGATATAAAAAAGGCGAGTTTCAAAACAATTTAAAATGAAGCTGACTTTGATTAGGAGATCATATGTTAGATGGACATCTATTTACAAATGTCTTACGAGTATCATGCATAGGTTTGTTGACTATTTTTTAAATTAAACACTCACTAATTTCTCAAAAAATAAAATTAAATTAAACGCTCACTAAAAATACTACTTTTCAAATTCCGGTGAGTTTATTTATTTATTTAATATTTGTTTGTATTTCAGGTATCCGGACAGAGACGTTGGACAGTATCCAATGGCGTACGTTGTGAGAAAGGGTGGAAATGACATTTCACATGAAGATGTAATGCAATTCATTGCAAAACAGGTCATTAAATTTCTCTGTTTTACAGTAACGTGAAAAATTCTCCTTCGAATTGTCAGCCGTTGTTGATAAAAAAAAAAAATTGTTTTAAGAACGATGCAATGTTTATAACATGTATAAAATGAAGTAGAGCTCGGTAAAAAAAAAAAAAAAAAAGAGTATTTTATAACAAAAATTTGATTTACTTGTGGAGTGAATATTTATTTATGGAATTGTACAGGTGGCACCGTACAAGAGAATCCGGCGGGTGGCTTTCGTGGACTCGATTCCGAAGAATCCGTCGGGGAAGATCCTGAGGAAGGATCTCATAAAGCTCGCGACCTCTAACTCTAAACTCTGAATCAACAAACGGAAGAATCAGTTGTCTTATCCACACGGAGCTGCTGGTCCCACTAGTAAATACTAATTCTGTTGATTGCGACGCTGTCATTTTCTATCTTCTGCGTTTACATTCTTTTTCTTCCGTAGATAATAAATACTGGTTTCCGGATTTCTCTCGACTATTTATGTTATTTAATACAATAGGTGGAGTAGGAATTTCAACTCACGACCCTTCTATCCGAGGTAGATACTTTGATCAGTTGAATTTTATTCGAGTGGGTAAGTTAATAGACGTTAAAATATAATAGGAATTTATTGCAGATACAACGTAATAAAAGTTGAACATATAGTATTCATGATTTGATTGGTAATTACTGTCAGATTTA

mRNA sequence

ATCGCAGACATTTATTTCCACACGTTCGGAATTCAATTTCCAGCATAATTTTCTCGTTAAAAAATTGCGAGCCGGAAATCGGAAGTCTAATAGTGGCAAATCCATACACGCTTTTTTTTAAAAAAATAATATAAACGAAATTATTATTTATTTTCAGAATTAAAAAACAGGTTGACCAGTCAACGACGGCGGCTCTGCACCTCCTATACATGCGAACGCGAGGACCTGCACGGCCACAAACAGTCTCCACCTTCTACTCTCACTTCTCTCTCGGACGGCGCCGGCGATTCACCCTTCTCCACCTACTTTTCCGACAGTCAAATGGCGCTCTACGGCGGCGGCGACAATCCCGCAGTGGATCCAAGAAGCGGCTTCTGCAGCTCCACTAAAATCTTCCACAGCAAACGCCGCCCAATCCCACTCCCACCCAAGCAATCTCTGAATGCGACGTCGTTTATCTCTTCCCGCCCCCACAACGGCAAAATCGCACTCATCGACGCCGCCACCGGCCAACATATCACCTACTCCCAACTCTGGGAATTCGTCGATTCCGTCGCCGCCGCTCTCTCCGACATCGGCATCAGAAAAGGCCACGTCATCCTCCTTCTATCCCCAAATTCAATTTTCTTCCCCATCATCTGCCTCGCAGTCATGTCCATCGGCGCAATCATCACCACAACCAACCCACTAAACACCGCCCAAGAAATCGCCAAACAAATCGCCGATTCGAAGCCGATTTTGGCCTTCACAACCCAGCAACTGATCCCCAAAATCGCCAGTTCGAATTTGCCCATAGTCCTAATCGACGGTCAAAATGAAAACCCCGCAGGAAATAAAGTCAAAATCGTGTCGAATTTGGGGGAAATGATGAGGAAAAATCCAAGCGGGAGCAGAATTACGGAGCCGGTGGAGCAGAACGACACAGCGACTCTGCTCTATTCCTCTGGAACGACAGGGGCGAGTAAAGGCGTGGTGTCGTCCCACAAGAATATCATAGCCATGGTTCAAGTCGTCGTGACGCGATTCAATTTAAGCGACGGGGAAGGAACTTTCATCTGTACAGTTCCGATGTTTCATATTTATGGCCTCGTGGCGTTCGCGACGGGGCTTCTCTCGTCGGGATCGACGATCGTTGTGTTGTCGAAATTCGAGATTCACGAAATGCTGTCGGCGATCGAGAGGTACCGGGCGACGTTTCTTCCCCTCGTGCCGCCGATTCTGGTAGCGCTCGTGAACGCCGCCGATCAGATCAAGGGGAAGTACGATTTGAGTTCGCTGCGTACGGCACTTTCCGGTGGAGCGCCGCTGGGGAAGGAGGTGATAGAGGGGTTTGTGGAGAAGTATCCGAATGTTACGATTCTTCAGGGCTACGGCTTAACGGAATCGACCGGAATTGGGGCGTCCACCGATTCTCTGGAGGAGAGCCGGCGGTACGGCACGGCGGGGCTTCTGTCGCCGAGCACGGAGGGGAAGATCGTGGACCCTGATAGCGGCGAAGCGCTGCCGGTGAAGCGGACGGGGGAGCTTTGGCTGAGAGGGCCCACCATAATGAAAGGGTATTTTGGTAACGTGGAGGCCACGACCTCAACACTTGACTCGGAGGGATGGTTGAGAACTGGAGATCTGTGTTACATTGATGAAGATGGTTTTATTTTTGTGGTTGATAGGCTTAAGGAACTCATCAAATATAAGGGTTATCAGGTCCCTCCTGCTGAATTAGAGGCTTTATTACTTACTCATCCTAATATCTCTGATGCAGCTGTGATTCCGTATCCGGACAGAGACGTTGGACAGTATCCAATGGCGTACGTTGTGAGAAAGGGTGGAAATGACATTTCACATGAAGATGTAATGCAATTCATTGCAAAACAGGTGGCACCGTACAAGAGAATCCGGCGGGTGGCTTTCGTGGACTCGATTCCGAAGAATCCGTCGGGGAAGATCCTGAGGAAGGATCTCATAAAGCTCGCGACCTCTAACTCTAAACTCTGAATCAACAAACGGAAGAATCAGTTGTCTTATCCACACGGAGCTGCTGGTCCCACTAGTAAATACTAATTCTGTTGATTGCGACGCTGTCATTTTCTATCTTCTGCGTTTACATTCTTTTTCTTCCGTAGATAATAAATACTGGTTTCCGGATTTCTCTCGACTATTTATGTTATTTAATACAATAGGTGGAGTAGGAATTTCAACTCACGACCCTTCTATCCGAGGTAGATACTTTGATCAGTTGAATTTTATTCGAGTGGGTAAGTTAATAGACGTTAAAATATAATAGGAATTTATTGCAGATACAACGTAATAAAAGTTGAACATATAGTATTCATGATTTGATTGGTAATTACTGTCAGATTTA

Coding sequence (CDS)

ATGGCGCTCTACGGCGGCGGCGACAATCCCGCAGTGGATCCAAGAAGCGGCTTCTGCAGCTCCACTAAAATCTTCCACAGCAAACGCCGCCCAATCCCACTCCCACCCAAGCAATCTCTGAATGCGACGTCGTTTATCTCTTCCCGCCCCCACAACGGCAAAATCGCACTCATCGACGCCGCCACCGGCCAACATATCACCTACTCCCAACTCTGGGAATTCGTCGATTCCGTCGCCGCCGCTCTCTCCGACATCGGCATCAGAAAAGGCCACGTCATCCTCCTTCTATCCCCAAATTCAATTTTCTTCCCCATCATCTGCCTCGCAGTCATGTCCATCGGCGCAATCATCACCACAACCAACCCACTAAACACCGCCCAAGAAATCGCCAAACAAATCGCCGATTCGAAGCCGATTTTGGCCTTCACAACCCAGCAACTGATCCCCAAAATCGCCAGTTCGAATTTGCCCATAGTCCTAATCGACGGTCAAAATGAAAACCCCGCAGGAAATAAAGTCAAAATCGTGTCGAATTTGGGGGAAATGATGAGGAAAAATCCAAGCGGGAGCAGAATTACGGAGCCGGTGGAGCAGAACGACACAGCGACTCTGCTCTATTCCTCTGGAACGACAGGGGCGAGTAAAGGCGTGGTGTCGTCCCACAAGAATATCATAGCCATGGTTCAAGTCGTCGTGACGCGATTCAATTTAAGCGACGGGGAAGGAACTTTCATCTGTACAGTTCCGATGTTTCATATTTATGGCCTCGTGGCGTTCGCGACGGGGCTTCTCTCGTCGGGATCGACGATCGTTGTGTTGTCGAAATTCGAGATTCACGAAATGCTGTCGGCGATCGAGAGGTACCGGGCGACGTTTCTTCCCCTCGTGCCGCCGATTCTGGTAGCGCTCGTGAACGCCGCCGATCAGATCAAGGGGAAGTACGATTTGAGTTCGCTGCGTACGGCACTTTCCGGTGGAGCGCCGCTGGGGAAGGAGGTGATAGAGGGGTTTGTGGAGAAGTATCCGAATGTTACGATTCTTCAGGGCTACGGCTTAACGGAATCGACCGGAATTGGGGCGTCCACCGATTCTCTGGAGGAGAGCCGGCGGTACGGCACGGCGGGGCTTCTGTCGCCGAGCACGGAGGGGAAGATCGTGGACCCTGATAGCGGCGAAGCGCTGCCGGTGAAGCGGACGGGGGAGCTTTGGCTGAGAGGGCCCACCATAATGAAAGGGTATTTTGGTAACGTGGAGGCCACGACCTCAACACTTGACTCGGAGGGATGGTTGAGAACTGGAGATCTGTGTTACATTGATGAAGATGGTTTTATTTTTGTGGTTGATAGGCTTAAGGAACTCATCAAATATAAGGGTTATCAGGTCCCTCCTGCTGAATTAGAGGCTTTATTACTTACTCATCCTAATATCTCTGATGCAGCTGTGATTCCGTATCCGGACAGAGACGTTGGACAGTATCCAATGGCGTACGTTGTGAGAAAGGGTGGAAATGACATTTCACATGAAGATGTAATGCAATTCATTGCAAAACAGGTGGCACCGTACAAGAGAATCCGGCGGGTGGCTTTCGTGGACTCGATTCCGAAGAATCCGTCGGGGAAGATCCTGAGGAAGGATCTCATAAAGCTCGCGACCTCTAACTCTAAACTCTGA

Protein sequence

MALYGGGDNPAVDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDAATGQHITYSQLWEFVDSVAAALSDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTAQEIAKQIADSKPILAFTTQQLIPKIASSNLPIVLIDGQNENPAGNKVKIVSNLGEMMRKNPSGSRITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDGEGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPILVALVNAADQIKGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGASTDSLEESRRYGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEATTSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAAVIPYPDRDVGQYPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLATSNSKL

Homology

[Close]

BLAST of MC11g0065 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 1.5e-239
Identity = 416/541 (76.89%), Postives = 470/541 (86.88%), Query Frame = 0


Query: 12  VDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDAATGQHITYSQL 71
           VD RSG+C S  IF+SKR P+ LP   S++ T+FISSR H+GKIA IDAATG+H+T+ QL
Sbjct: 9   VDERSGYCKSNSIFYSKREPVQLPQNHSVDVTTFISSRAHHGKIAFIDAATGRHLTFPQL 68

Query: 72  WEFVDSVAAALSDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTAQEIAK 131
           W  VDSVA  LS +GIRKG VILLLSPNSI+FP++CLAVMS+GAIITTTNPLNT +EIAK
Sbjct: 69  WRAVDSVATCLSAMGIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPREIAK 128

Query: 132 QIADSKPILAFTTQQLIPKIASSNLPIVLIDGQNENPAGNKVKIVSNLGEMMRKNPSGSR 191
           QI DSKP+LAFT  QL+ KIA SNLPIV+ID + ++     + IVS+LGEMMRK PS +R
Sbjct: 129 QITDSKPVLAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKTLNIVSSLGEMMRKEPSPNR 188

Query: 192 ITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDGEGTFICTVPMF 251
           I   V Q DTATLLYSSGTTGASKGVVSSHKN+IAMVQ +++RF   DGE TFICTVPMF
Sbjct: 189 IGYRVNQEDTATLLYSSGTTGASKGVVSSHKNLIAMVQTILSRFGTEDGEHTFICTVPMF 248

Query: 252 HIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPILVALVNAADQIK 311
           HIYGL AFA GLLSSGSTIV+LSKFEIHEMLSAIE+YRAT+LPLVPPIL+AL+  A+ I+
Sbjct: 249 HIYGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPILMALLKNANHIR 308

Query: 312 GKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGASTDSLEESRRY 371
            KYDLSSL++ LSGGAPL KEVIEGFVE YP V+ILQGYGLTESTGIGASTD L+ESRRY
Sbjct: 309 AKYDLSSLQSVLSGGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIGASTDCLQESRRY 368

Query: 372 GTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEATTSTLDSEGWLR 431
           GTAG+LSPS E KIV+P++GEAL V RTGELWLRGPTIMKGYF N EAT+ST+DSEGWLR
Sbjct: 369 GTAGMLSPSMEAKIVNPETGEALSVNRTGELWLRGPTIMKGYFSNEEATSSTIDSEGWLR 428

Query: 432 TGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAAVIPYPDRDVGQ 491
           TGDLCYIDEDGFIFVVDRLKELIKYKGYQV PAELEALLL+HP ISDAAVIPYPD++ GQ
Sbjct: 429 TGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLSHPEISDAAVIPYPDKEAGQ 488

Query: 492 YPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLAT 551
           +PMAYVVRKGG+++S   VM FIAK VAPYKRIR+VAFV SIPKNPSGKILRKDLIKLAT
Sbjct: 489 FPMAYVVRKGGSNLSESTVMDFIAKLVAPYKRIRKVAFVASIPKNPSGKILRKDLIKLAT 548

Query: 552 S 553
           S
Sbjct: 549 S 549

[Close]

BLAST of MC11g0065 vs. ExPASy Swiss-Prot
Match: Q84P21 (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 809.3 bits (2089), Expect = 2.7e-233
Identity = 403/548 (73.54%), Postives = 471/548 (85.95%), Query Frame = 0


Query: 11  AVDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDAATGQHITYSQ 70
           +V+ RSGFC+S   F+SKR PIPLPP  SL+ T+FISS+ H G+IA IDA+TGQ++T+++
Sbjct: 3   SVNSRSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFISSQAHRGRIAFIDASTGQNLTFTE 62

Query: 71  LWEFVDSVAAALSDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTAQEIA 130
           LW  V+SVA  LS+IGIRKGHV+LLLSPNSI FP++CL+VMS+GAIITTTNPLNT+ EIA
Sbjct: 63  LWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIA 122

Query: 131 KQIADSKPILAFTTQQLIPKI--ASSNLPIVLIDGQNENPAGNKVKIVSNLGEMMRKNPS 190
           KQI DS P+LAFTT QL+PKI  A+  LPIVL+D +  +  G+    V  L EMM+K PS
Sbjct: 123 KQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGD----VRRLVEMMKKEPS 182

Query: 191 GSRITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDGEGTFICTV 250
           G+R+ E V+Q+DTATLLYSSGTTG SKGV+SSH+N+IAMVQ +V RF   DGE  FICTV
Sbjct: 183 GNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFICTV 242

Query: 251 PMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPILVALVNAAD 310
           PMFHIYGL AFATGLL+ GSTI+VLSKFE+HEM+SAI +Y+AT LPLVPPILVA+VN AD
Sbjct: 243 PMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGAD 302

Query: 311 QIKGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGASTDSLEES 370
           QIK KYDLSS+ T L GGAPL KEV EGF EKYP V ILQGYGLTESTGIGASTD++EES
Sbjct: 303 QIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEES 362

Query: 371 RRYGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEATTSTLDSEG 430
           RRYGTAG LS S EG+IVDP +G+ L  K+TGELWL+GP+IMKGYF N EAT+STLDSEG
Sbjct: 363 RRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEG 422

Query: 431 WLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAAVIPYPDRD 490
           WLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQV PAELEALLLTHP I+DAAVIP+PD++
Sbjct: 423 WLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKE 482

Query: 491 VGQYPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIK 550
           VGQ+PMAYVVRK G+ +S + +M+F+AKQVAPYKRIR+VAFV SIPKNPSGKILRKDLIK
Sbjct: 483 VGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLIK 542

Query: 551 LATSNSKL 557
           +ATSNSKL
Sbjct: 543 IATSNSKL 546

[Close]

BLAST of MC11g0065 vs. ExPASy Swiss-Prot
Match: Q10S72 (4-coumarate--CoA ligase-like 4 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL4 PE=2 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 4.7e-201
Identity = 347/546 (63.55%), Postives = 438/546 (80.22%), Query Frame = 0


Query: 12  VDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDAATGQHITYSQL 71
           VD RSG+C++T+ F S+R  +PLP    ++  SF++SR H+G +AL+DAATG+ IT+++L
Sbjct: 9   VDARSGYCAATRTFRSRRADVPLPADPEVDVVSFLASRRHSGVVALVDAATGRRITFTEL 68

Query: 72  WEFVDSVAAALS--DIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTAQEI 131
           W  V   A+AL+   + +RKGHV L+LSPNS+ FP+  LA MS+GA++TT NPLNT  EI
Sbjct: 69  WRAVAGAASALAAHPVSLRKGHVALILSPNSVHFPVAALAAMSLGAVLTTANPLNTPAEI 128

Query: 132 AKQIADSKPILAFTTQQLIPKI-ASSNLPIVLIDGQNENPAGNKVKIVSNLGEMMRKNPS 191
           AKQ+AD++P+LAFTT++L+PK+  + +L +VL++        +  +IV+ + E+    P 
Sbjct: 129 AKQVADARPVLAFTTRELLPKLPRAHDLRVVLLESARLPGDSSDPRIVATIEEISATTPD 188

Query: 192 GSRITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNL--SDGEGTFIC 251
            +R  + V Q+D ATLLYSSGTTG SKGVV++H+++I+MVQ+++TRF L  SD   TF+C
Sbjct: 189 PARRKDRVTQDDPATLLYSSGTTGPSKGVVATHRSLISMVQIIMTRFRLEGSDKTETFLC 248

Query: 252 TVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPILVALVNA 311
           TVPMFH+YGLVAFATGLL  G+T+VVLSK+E+ EML +I  Y  T+LPLVPPILVA+V  
Sbjct: 249 TVPMFHVYGLVAFATGLLGCGATVVVLSKYELPEMLRSINAYGVTYLPLVPPILVAMVAH 308

Query: 312 ADQIKGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGASTDSLE 371
              +     L  +R  LSGGAPLGKE+IEGF EKYP V ILQGYGLTEST IGASTDS E
Sbjct: 309 PKPL----PLGQMRKVLSGGAPLGKELIEGFREKYPQVEILQGYGLTESTAIGASTDSAE 368

Query: 372 ESRRYGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEATTSTLDS 431
           ESRRYGTAGLLSP+TE KIVDPDSGEALPV RTGELW+RGP +MKGYF N EAT STL  
Sbjct: 369 ESRRYGTAGLLSPNTEAKIVDPDSGEALPVNRTGELWIRGPYVMKGYFKNAEATQSTLTP 428

Query: 432 EGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAAVIPYPD 491
           +GWL+TGDLCYIDEDG++FVVDRLKELIKYKGYQVPPAELEALLLTHP ++D AVIP+PD
Sbjct: 429 DGWLKTGDLCYIDEDGYLFVVDRLKELIKYKGYQVPPAELEALLLTHPEVTDVAVIPFPD 488

Query: 492 RDVGQYPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGKILRKDL 551
           R+VGQ+PMAY+VRK G+++S  +VM+F+AKQVAPYK++R+VAFV  IPKN SGKILRKDL
Sbjct: 489 REVGQFPMAYIVRKKGSNLSEREVMEFVAKQVAPYKKVRKVAFVTDIPKNASGKILRKDL 548

Query: 552 IKLATS 553
           IKLATS
Sbjct: 549 IKLATS 550

[Close]

BLAST of MC11g0065 vs. ExPASy Swiss-Prot
Match: P0C5B6 (OPC-6:CoA ligase OS=Arabidopsis thaliana OX=3702 GN=4CLL4 PE=2 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 8.5e-195
Identity = 338/545 (62.02%), Postives = 427/545 (78.35%), Query Frame = 0


Query: 12  VDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDAATGQHITYSQL 71
           VDPRSGFC S   F+SKR+P+ LPP  S + T+FISS+PH GK A IDAATGQ +T+S L
Sbjct: 10  VDPRSGFCKSNSTFYSKRQPLSLPPNLSRDVTTFISSQPHRGKTAFIDAATGQCLTFSDL 69

Query: 72  WEFVDSVAAAL-SDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTAQEIA 131
           W  VD VA  L  ++GIR+G V+L+LSPNSIF P++CL+VMS+GA+ TT N LNT+ EI+
Sbjct: 70  WRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEIS 129

Query: 132 KQIADSKPILAFTTQQLIPKIASSNLPIVLIDGQNENPAGNKVKIVSNLGEMMRKNPSGS 191
           KQIADS P L FTT+QL PK+  + + +VL D +      + +++V  L EM++K PSG 
Sbjct: 130 KQIADSNPTLVFTTRQLAPKLPVA-ISVVLTDDEVYQELTSAIRVVGILSEMVKKEPSGQ 189

Query: 192 RITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDG---EGTFICT 251
           R+ + V Q+DTA +LYSSGTTG SKGV+SSH+N+ A     V RF +SD    +  FICT
Sbjct: 190 RVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAH----VARF-ISDNLKRDDIFICT 249

Query: 252 VPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPILVALVNAA 311
           VPMFH YGL+ FA G ++ GST+V+L +F++H+M+ A+E++RAT L L PP+LVA++N A
Sbjct: 250 VPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPVLVAMINDA 309

Query: 312 DQIKGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGASTDSLEE 371
           D IK KYDLSSL+T   GGAPL KEV EGF+EKYP V ILQGY LTES G GA T+S EE
Sbjct: 310 DLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGGGAFTNSAEE 369

Query: 372 SRRYGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEATTSTLDSE 431
           SRRYGTAG L+   E +IVDP++G  + + +TGELWL+GP+I KGYF N EAT  T++ E
Sbjct: 370 SRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEATNETINLE 429

Query: 432 GWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAAVIPYPDR 491
           GWL+TGDLCYIDEDGF+FVVDRLKELIKYKGYQVPPAELEALL+THP+I DAAVIP+PD+
Sbjct: 430 GWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILDAAVIPFPDK 489

Query: 492 DVGQYPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGKILRKDLI 551
           + GQYPMAYVVRK  +++S + V+ FI+KQVAPYK+IR+V+F++SIPK  SGK LRKDLI
Sbjct: 490 EAGQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFINSIPKTASGKTLRKDLI 548

Query: 552 KLATS 553
           KLATS
Sbjct: 550 KLATS 548

[Close]

BLAST of MC11g0065 vs. ExPASy Swiss-Prot
Match: Q84P25 (4-coumarate--CoA ligase-like 2 OS=Arabidopsis thaliana OX=3702 GN=4CLL2 PE=2 SV=2)

HSP 1 Score: 676.8 bits (1745), Expect = 2.1e-193
Identity = 335/547 (61.24%), Postives = 428/547 (78.24%), Query Frame = 0


Query: 11  AVDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDAATGQHITYSQ 70
           AVD +SGFC ST IF+SKR P+ LPP Q L+ TSFI+S+PH GK   +DA TG+ +++ +
Sbjct: 20  AVDRKSGFCESTSIFYSKREPMALPPNQFLDVTSFIASQPHRGKTVFVDAVTGRRLSFPE 79

Query: 71  LWEFVDSVAAALSDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTAQEIA 130
           LW  V+ VA  L  +G+RKG+V+++LSPNSI FPI+ L+VMS+GAIITT NP+NT+ EI+
Sbjct: 80  LWLGVERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEIS 139

Query: 131 KQIADSKPILAFTTQQLIPKIASS---NLPIVLIDGQN--ENPAGNKVKIVSNLGEMMRK 190
           KQI DS+P+LAFTT +L+ K+A++   NLP+VL+D  +      G++VK+V  L  M+  
Sbjct: 140 KQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIET 199

Query: 191 NPSGSRITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDGEGTFI 250
            PS SR+ + V Q+DTA LLYSSGTTG SKGV+ SH+N+IA+VQ    RF L   E   I
Sbjct: 200 EPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYRARFGL---EQRTI 259

Query: 251 CTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPILVALVN 310
           CT+PM HI+G   FATGL++ G TIVVL KF++ ++LSA+E +R+++L LVPPI+VA+VN
Sbjct: 260 CTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMVN 319

Query: 311 AADQIKGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGASTDSL 370
            A++I  KYDLSSL T ++GGAPL +EV E FVE YP V ILQGYGLTEST I AS  + 
Sbjct: 320 GANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFNK 379

Query: 371 EESRRYGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEATTSTLD 430
           EE++RYG +GLL+P+ EGKIVDPD+G  L V +TGELW+R PT+MKGYF N EAT ST+D
Sbjct: 380 EETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTID 439

Query: 431 SEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAAVIPYP 490
           SEGWL+TGDLCYID DGF+FVVDRLKELIK  GYQV PAELEALLL HP I+DAAVIP P
Sbjct: 440 SEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPIP 499

Query: 491 DRDVGQYPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGKILRKD 550
           D   GQYPMAY+VRK G+++S  ++M F+AKQV+PYK+IR+V F+ SIPKNPSGKILR++
Sbjct: 500 DMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKILRRE 559

Query: 551 LIKLATS 553
           L KL TS
Sbjct: 560 LTKLTTS 563

[Close]

BLAST of MC11g0065 vs. NCBI nr
Match: XP_022936511.1 (4-coumarate--CoA ligase-like 5 [Cucurbita moschata])

HSP 1 Score: 977 bits (2526), Expect = 0.0
Identity = 495/552 (89.67%), Postives = 525/552 (95.11%), Query Frame = 0


Query: 1   MALYGGGDNPAVDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDA 60
           MA+YGG D P VDPRSGFC STKIFHSKRRPIPLPP QSL+AT+FISSRPHNGKIALIDA
Sbjct: 1   MAVYGG-DVPEVDPRSGFCKSTKIFHSKRRPIPLPPNQSLDATTFISSRPHNGKIALIDA 60

Query: 61  ATGQHITYSQLWEFVDSVAAALSDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTT 120
            TGQHITYS LWE VDSVA++LSD+GIRKGHVILLLSPNSIFFPIICLAVMS+GA+ITTT
Sbjct: 61  TTGQHITYSHLWESVDSVASSLSDMGIRKGHVILLLSPNSIFFPIICLAVMSLGAVITTT 120

Query: 121 NPLNTAQEIAKQIADSKPILAFTTQQLIPKIASSNLPIVLIDGQNENPAGNKVKIVSNLG 180
           NPLNT QEIAKQIADS PILAFTTQQLIPKIASS LP+VLIDG+ +    + VKIVS LG
Sbjct: 121 NPLNTPQEIAKQIADSNPILAFTTQQLIPKIASSKLPVVLIDGEIQIKKFS-VKIVSTLG 180

Query: 181 EMMRKNPSGSRITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDG 240
           EMMRK  SGSR+ E V+QNDTATLLYSSGTTGASKGVVSSHKN+IAMVQVVVTRF LS+G
Sbjct: 181 EMMRKKSSGSRVKERVDQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFKLSEG 240

Query: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPIL 300
           EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIE+YRAT+LPLVPPIL
Sbjct: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIEKYRATYLPLVPPIL 300

Query: 301 VALVNAADQIKGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGA 360
           VA+VNAA+QIKGKYDL SL TALSGGAPLGKEVIEGFVEKYPNV+ILQGYGLTESTGIGA
Sbjct: 301 VAMVNAAEQIKGKYDLGSLHTALSGGAPLGKEVIEGFVEKYPNVSILQGYGLTESTGIGA 360

Query: 361 STDSLEESRRYGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEAT 420
           STDSLEESRRYGTAGLLSPSTE KIVDPD+GEALPV RTGELWLRGPTIMKGYFGNVEAT
Sbjct: 361 STDSLEESRRYGTAGLLSPSTEAKIVDPDTGEALPVNRTGELWLRGPTIMKGYFGNVEAT 420

Query: 421 TSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAA 480
           TSTLDSEGWLRTGDLCY+DEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHP+ISDAA
Sbjct: 421 TSTLDSEGWLRTGDLCYVDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPSISDAA 480

Query: 481 VIPYPDRDVGQYPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGK 540
           VIPYPD++VGQYPMAYVVRKGG+DISHED+MQF+A+QVAPYKRIRRVAFVDSIPKNPSGK
Sbjct: 481 VIPYPDKEVGQYPMAYVVRKGGSDISHEDIMQFVARQVAPYKRIRRVAFVDSIPKNPSGK 540

Query: 541 ILRKDLIKLATS 552
           ILRKDLIKLATS
Sbjct: 541 ILRKDLIKLATS 550

[Close]

BLAST of MC11g0065 vs. NCBI nr
Match: XP_022982315.1 (4-coumarate--CoA ligase-like 5 isoform X1 [Cucurbita maxima])

HSP 1 Score: 968 bits (2502), Expect = 0.0
Identity = 494/552 (89.49%), Postives = 518/552 (93.84%), Query Frame = 0


Query: 1   MALYGGGDNPAVDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDA 60
           MA +GG     +DPRSGFC STKIFHSKR PIPLPP QSL+AT+FISSRPHNGKIALIDA
Sbjct: 1   MAEHGG----EIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDA 60

Query: 61  ATGQHITYSQLWEFVDSVAAALSDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTT 120
            TGQHITYS LWE +DSVA+ALSD+GIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTT
Sbjct: 61  TTGQHITYSHLWESLDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTT 120

Query: 121 NPLNTAQEIAKQIADSKPILAFTTQQLIPKIASSNLPIVLIDGQNENPAGNKVKIVSNLG 180
           NPLNT QEIAKQIADSKPILAFTTQQL+PKIASS LPIVLIDGQ + P+   VKIVS L 
Sbjct: 121 NPLNTPQEIAKQIADSKPILAFTTQQLLPKIASSTLPIVLIDGQIQTPS---VKIVSTLN 180

Query: 181 EMMRKNPSGSRITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDG 240
           EMMRK PS SRI E VEQNDTATLLYSSGTTGASKGVVSSHKN+IAMVQVVVTRF LS+G
Sbjct: 181 EMMRKKPSESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEG 240

Query: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPIL 300
           EGTFICTVPMFHIYGLVAFATGLLSSGSTI+VLSKFEIHEMLSAIE+YRAT+LPLVPPIL
Sbjct: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIEKYRATYLPLVPPIL 300

Query: 301 VALVNAADQIKGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGA 360
           VALVNAA+QIKGKYDLSSL TALSGGAPLGKEVIEGFVEKYPNV ILQGYGLTESTGIGA
Sbjct: 301 VALVNAAEQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGA 360

Query: 361 STDSLEESRRYGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEAT 420
           STDSLEESRRYGTAGLLSPSTE  IVDP++GEALPV RTGELWLRGPT+MKGYFGNVEAT
Sbjct: 361 STDSLEESRRYGTAGLLSPSTEAMIVDPETGEALPVNRTGELWLRGPTVMKGYFGNVEAT 420

Query: 421 TSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAA 480
            STLDS GWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLL HPNISDAA
Sbjct: 421 ASTLDSMGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLAHPNISDAA 480

Query: 481 VIPYPDRDVGQYPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGK 540
           VIP+PD++VGQYPMAYVVRKGG+DISHEDVMQF+AKQVAPYKRIRRVAFVDSIPKNPSGK
Sbjct: 481 VIPFPDKEVGQYPMAYVVRKGGSDISHEDVMQFVAKQVAPYKRIRRVAFVDSIPKNPSGK 540

Query: 541 ILRKDLIKLATS 552
           ILRKDLIKLATS
Sbjct: 541 ILRKDLIKLATS 545

[Close]

BLAST of MC11g0065 vs. NCBI nr
Match: XP_023522465.1 (4-coumarate--CoA ligase-like 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 967 bits (2501), Expect = 0.0
Identity = 493/552 (89.31%), Postives = 519/552 (94.02%), Query Frame = 0


Query: 1   MALYGGGDNPAVDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDA 60
           MA +GG     +DPRSGFC STKIFHSKR PIPLPP QSL+AT+FISSRPHNGKIALIDA
Sbjct: 1   MAEHGG----EIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDA 60

Query: 61  ATGQHITYSQLWEFVDSVAAALSDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTT 120
            TGQHITYS LWE VDSVA+ALSD+GIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTT
Sbjct: 61  TTGQHITYSHLWEAVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTT 120

Query: 121 NPLNTAQEIAKQIADSKPILAFTTQQLIPKIASSNLPIVLIDGQNENPAGNKVKIVSNLG 180
           NPLNT QEIAKQIADSKPILAFTTQQL+PKIASS LPIVLIDG+   P+   VKIVS L 
Sbjct: 121 NPLNTPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIHTPS---VKIVSTLN 180

Query: 181 EMMRKNPSGSRITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDG 240
           EMMRK PS SRI E VEQNDTATLLYSSGTTGASKGVVSSHKN+IAMVQVVVTRF LS+G
Sbjct: 181 EMMRKKPSESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEG 240

Query: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPIL 300
           EGTFICTVPMFHIYGLVAFATGLLSSGSTI+VLSKFEIHEMLSAIE+YRAT+LPLVPPIL
Sbjct: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIEKYRATYLPLVPPIL 300

Query: 301 VALVNAADQIKGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGA 360
           VALVNAA+QIKGKYDLSSL TALSGGAPLGKEVIEGFVEKYPNV ILQGYGLTESTGIGA
Sbjct: 301 VALVNAAEQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGA 360

Query: 361 STDSLEESRRYGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEAT 420
           STDSLEESRRYGTAGLLSPSTE  IV+P++GEALPV RTGELWLRGPT+MKGYFGNVEAT
Sbjct: 361 STDSLEESRRYGTAGLLSPSTEAMIVEPETGEALPVNRTGELWLRGPTVMKGYFGNVEAT 420

Query: 421 TSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAA 480
            STLDS+GWLRTGDLCYID+DGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAA
Sbjct: 421 ASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAA 480

Query: 481 VIPYPDRDVGQYPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGK 540
           VIP+PD++VGQYPMAYVVRKGG+DISHEDVMQF+AKQVAPYKRIRRVAFVDSIPKNPSGK
Sbjct: 481 VIPFPDKEVGQYPMAYVVRKGGSDISHEDVMQFVAKQVAPYKRIRRVAFVDSIPKNPSGK 540

Query: 541 ILRKDLIKLATS 552
           ILRKDLIKLATS
Sbjct: 541 ILRKDLIKLATS 545

[Close]

BLAST of MC11g0065 vs. NCBI nr
Match: XP_022975900.1 (4-coumarate--CoA ligase-like 5 [Cucurbita maxima])

HSP 1 Score: 967 bits (2500), Expect = 0.0
Identity = 492/552 (89.13%), Postives = 521/552 (94.38%), Query Frame = 0


Query: 1   MALYGGGDNPAVDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDA 60
           MA+YGG D P VDPRSGFC STKIFHSKRRPIPLPP QSL+AT+FISSRPHNGKIALIDA
Sbjct: 1   MAVYGG-DVPQVDPRSGFCKSTKIFHSKRRPIPLPPNQSLDATTFISSRPHNGKIALIDA 60

Query: 61  ATGQHITYSQLWEFVDSVAAALSDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTT 120
            TGQHITYS LWE VDSVA++LSD+GIRKGHVILLLSPNSIFFPIICLAVMS+GA+ITTT
Sbjct: 61  TTGQHITYSHLWESVDSVASSLSDMGIRKGHVILLLSPNSIFFPIICLAVMSLGAVITTT 120

Query: 121 NPLNTAQEIAKQIADSKPILAFTTQQLIPKIASSNLPIVLIDGQNENPAGNKVKIVSNLG 180
           NPLNT QEIAKQIADS PILAFTTQQLIPKIASS LP+VLIDG+ +    + VKIVS L 
Sbjct: 121 NPLNTPQEIAKQIADSNPILAFTTQQLIPKIASSKLPVVLIDGEIQIKKFS-VKIVSTLS 180

Query: 181 EMMRKNPSGSRITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDG 240
           EMMRK  SGSR+ E V+QNDTATLLYSSGTTGASKGVVSSHKN+IAMVQVVVTRF LS+G
Sbjct: 181 EMMRKKSSGSRVKERVDQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFKLSEG 240

Query: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPIL 300
           EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIE+YRAT+LPLVPPIL
Sbjct: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIEKYRATYLPLVPPIL 300

Query: 301 VALVNAADQIKGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGA 360
           VA+VNAA+QIKGKYDL SL TALSGGAPLGKEVIEGFVEKYPNV+ILQGYGLTESTGIGA
Sbjct: 301 VAMVNAAEQIKGKYDLGSLHTALSGGAPLGKEVIEGFVEKYPNVSILQGYGLTESTGIGA 360

Query: 361 STDSLEESRRYGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEAT 420
           STDSLEESRRYGTAGLLSPSTE KIV PD+GEALPV  TGELWLRGPTIMKGYFGNVEAT
Sbjct: 361 STDSLEESRRYGTAGLLSPSTEAKIVVPDTGEALPVNTTGELWLRGPTIMKGYFGNVEAT 420

Query: 421 TSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAA 480
           TSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHP+ISDAA
Sbjct: 421 TSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPSISDAA 480

Query: 481 VIPYPDRDVGQYPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGK 540
           VIPYPD++VGQYPMAYVVRKGG+DISHED+MQ +A+QVAPYKRIRRVAFVDSIPKNPSGK
Sbjct: 481 VIPYPDKEVGQYPMAYVVRKGGSDISHEDIMQLVARQVAPYKRIRRVAFVDSIPKNPSGK 540

Query: 541 ILRKDLIKLATS 552
           ILRKDLIKLATS
Sbjct: 541 ILRKDLIKLATS 550

[Close]

BLAST of MC11g0065 vs. NCBI nr
Match: XP_023534989.1 (4-coumarate--CoA ligase-like 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 965 bits (2495), Expect = 0.0
Identity = 491/552 (88.95%), Postives = 520/552 (94.20%), Query Frame = 0


Query: 1   MALYGGGDNPAVDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDA 60
           MA+YGG D P VDPRSGFC STKIFHSKRRPIPLPP QSL+AT+FISSRPHNGKIALIDA
Sbjct: 1   MAVYGG-DVPEVDPRSGFCKSTKIFHSKRRPIPLPPNQSLDATTFISSRPHNGKIALIDA 60

Query: 61  ATGQHITYSQLWEFVDSVAAALSDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTT 120
            TGQHITYS LWE VDSVA++LSD+GIRKGHVILLLSPNSIFFPIICLAVMS+GA+ITTT
Sbjct: 61  TTGQHITYSHLWESVDSVASSLSDMGIRKGHVILLLSPNSIFFPIICLAVMSLGAVITTT 120

Query: 121 NPLNTAQEIAKQIADSKPILAFTTQQLIPKIASSNLPIVLIDGQNENPAGNKVKIVSNLG 180
           NPLNT QEIAKQIADS PILAFTTQQLIPKIASS LP+VLIDG+ +      VKIVS L 
Sbjct: 121 NPLNTPQEIAKQIADSNPILAFTTQQLIPKIASSKLPVVLIDGEIQIKKF-PVKIVSTLS 180

Query: 181 EMMRKNPSGSRITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDG 240
           +MMRK  SGSR+ E V+QNDTATLLYSSGTTGASKGVVSSHKN+IAMVQVVVTRF LS+G
Sbjct: 181 DMMRKKSSGSRVKERVDQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFKLSEG 240

Query: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPIL 300
           EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIE+YRAT+LPLVPPIL
Sbjct: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIEKYRATYLPLVPPIL 300

Query: 301 VALVNAADQIKGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGA 360
           VA+VNAA+QIKGKYDL SL TALSGGAPLGKEVIEGFVEKYPNV+ILQGYGLTESTGIGA
Sbjct: 301 VAMVNAAEQIKGKYDLGSLHTALSGGAPLGKEVIEGFVEKYPNVSILQGYGLTESTGIGA 360

Query: 361 STDSLEESRRYGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEAT 420
           STDSLEESRRYGTAGLLSPSTE KIVDPD+G+ALPV RTGELWLRGPTIMKGYFGNVEAT
Sbjct: 361 STDSLEESRRYGTAGLLSPSTEAKIVDPDTGDALPVNRTGELWLRGPTIMKGYFGNVEAT 420

Query: 421 TSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAA 480
           TSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQV  AELEALLLTHP+ISDAA
Sbjct: 421 TSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVASAELEALLLTHPSISDAA 480

Query: 481 VIPYPDRDVGQYPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGK 540
           VIPYPD++VGQYPMAYVVRKGG+DISHED MQF+A+QVAPYKRIRRVAFVDSIPKNPSGK
Sbjct: 481 VIPYPDKEVGQYPMAYVVRKGGSDISHEDTMQFVARQVAPYKRIRRVAFVDSIPKNPSGK 540

Query: 541 ILRKDLIKLATS 552
           ILRKDLIKLATS
Sbjct: 541 ILRKDLIKLATS 550

[Close]

BLAST of MC11g0065 vs. ExPASy TrEMBL
Match: A0A6J1F7P0 (4-coumarate--CoA ligase-like 5 OS=Cucurbita moschata OX=3662 GN=LOC111443102 PE=4 SV=1)

HSP 1 Score: 977 bits (2526), Expect = 0.0
Identity = 495/552 (89.67%), Postives = 525/552 (95.11%), Query Frame = 0


Query: 1   MALYGGGDNPAVDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDA 60
           MA+YGG D P VDPRSGFC STKIFHSKRRPIPLPP QSL+AT+FISSRPHNGKIALIDA
Sbjct: 1   MAVYGG-DVPEVDPRSGFCKSTKIFHSKRRPIPLPPNQSLDATTFISSRPHNGKIALIDA 60

Query: 61  ATGQHITYSQLWEFVDSVAAALSDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTT 120
            TGQHITYS LWE VDSVA++LSD+GIRKGHVILLLSPNSIFFPIICLAVMS+GA+ITTT
Sbjct: 61  TTGQHITYSHLWESVDSVASSLSDMGIRKGHVILLLSPNSIFFPIICLAVMSLGAVITTT 120

Query: 121 NPLNTAQEIAKQIADSKPILAFTTQQLIPKIASSNLPIVLIDGQNENPAGNKVKIVSNLG 180
           NPLNT QEIAKQIADS PILAFTTQQLIPKIASS LP+VLIDG+ +    + VKIVS LG
Sbjct: 121 NPLNTPQEIAKQIADSNPILAFTTQQLIPKIASSKLPVVLIDGEIQIKKFS-VKIVSTLG 180

Query: 181 EMMRKNPSGSRITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDG 240
           EMMRK  SGSR+ E V+QNDTATLLYSSGTTGASKGVVSSHKN+IAMVQVVVTRF LS+G
Sbjct: 181 EMMRKKSSGSRVKERVDQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFKLSEG 240

Query: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPIL 300
           EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIE+YRAT+LPLVPPIL
Sbjct: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIEKYRATYLPLVPPIL 300

Query: 301 VALVNAADQIKGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGA 360
           VA+VNAA+QIKGKYDL SL TALSGGAPLGKEVIEGFVEKYPNV+ILQGYGLTESTGIGA
Sbjct: 301 VAMVNAAEQIKGKYDLGSLHTALSGGAPLGKEVIEGFVEKYPNVSILQGYGLTESTGIGA 360

Query: 361 STDSLEESRRYGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEAT 420
           STDSLEESRRYGTAGLLSPSTE KIVDPD+GEALPV RTGELWLRGPTIMKGYFGNVEAT
Sbjct: 361 STDSLEESRRYGTAGLLSPSTEAKIVDPDTGEALPVNRTGELWLRGPTIMKGYFGNVEAT 420

Query: 421 TSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAA 480
           TSTLDSEGWLRTGDLCY+DEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHP+ISDAA
Sbjct: 421 TSTLDSEGWLRTGDLCYVDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPSISDAA 480

Query: 481 VIPYPDRDVGQYPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGK 540
           VIPYPD++VGQYPMAYVVRKGG+DISHED+MQF+A+QVAPYKRIRRVAFVDSIPKNPSGK
Sbjct: 481 VIPYPDKEVGQYPMAYVVRKGGSDISHEDIMQFVARQVAPYKRIRRVAFVDSIPKNPSGK 540

Query: 541 ILRKDLIKLATS 552
           ILRKDLIKLATS
Sbjct: 541 ILRKDLIKLATS 550

[Close]

BLAST of MC11g0065 vs. ExPASy TrEMBL
Match: A0A6J1J2B8 (4-coumarate--CoA ligase-like 5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481187 PE=4 SV=1)

HSP 1 Score: 968 bits (2502), Expect = 0.0
Identity = 494/552 (89.49%), Postives = 518/552 (93.84%), Query Frame = 0


Query: 1   MALYGGGDNPAVDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDA 60
           MA +GG     +DPRSGFC STKIFHSKR PIPLPP QSL+AT+FISSRPHNGKIALIDA
Sbjct: 1   MAEHGG----EIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDA 60

Query: 61  ATGQHITYSQLWEFVDSVAAALSDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTT 120
            TGQHITYS LWE +DSVA+ALSD+GIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTT
Sbjct: 61  TTGQHITYSHLWESLDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTT 120

Query: 121 NPLNTAQEIAKQIADSKPILAFTTQQLIPKIASSNLPIVLIDGQNENPAGNKVKIVSNLG 180
           NPLNT QEIAKQIADSKPILAFTTQQL+PKIASS LPIVLIDGQ + P+   VKIVS L 
Sbjct: 121 NPLNTPQEIAKQIADSKPILAFTTQQLLPKIASSTLPIVLIDGQIQTPS---VKIVSTLN 180

Query: 181 EMMRKNPSGSRITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDG 240
           EMMRK PS SRI E VEQNDTATLLYSSGTTGASKGVVSSHKN+IAMVQVVVTRF LS+G
Sbjct: 181 EMMRKKPSESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEG 240

Query: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPIL 300
           EGTFICTVPMFHIYGLVAFATGLLSSGSTI+VLSKFEIHEMLSAIE+YRAT+LPLVPPIL
Sbjct: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIEKYRATYLPLVPPIL 300

Query: 301 VALVNAADQIKGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGA 360
           VALVNAA+QIKGKYDLSSL TALSGGAPLGKEVIEGFVEKYPNV ILQGYGLTESTGIGA
Sbjct: 301 VALVNAAEQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGA 360

Query: 361 STDSLEESRRYGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEAT 420
           STDSLEESRRYGTAGLLSPSTE  IVDP++GEALPV RTGELWLRGPT+MKGYFGNVEAT
Sbjct: 361 STDSLEESRRYGTAGLLSPSTEAMIVDPETGEALPVNRTGELWLRGPTVMKGYFGNVEAT 420

Query: 421 TSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAA 480
            STLDS GWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLL HPNISDAA
Sbjct: 421 ASTLDSMGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLAHPNISDAA 480

Query: 481 VIPYPDRDVGQYPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGK 540
           VIP+PD++VGQYPMAYVVRKGG+DISHEDVMQF+AKQVAPYKRIRRVAFVDSIPKNPSGK
Sbjct: 481 VIPFPDKEVGQYPMAYVVRKGGSDISHEDVMQFVAKQVAPYKRIRRVAFVDSIPKNPSGK 540

Query: 541 ILRKDLIKLATS 552
           ILRKDLIKLATS
Sbjct: 541 ILRKDLIKLATS 545

[Close]

BLAST of MC11g0065 vs. ExPASy TrEMBL
Match: A0A6J1II10 (4-coumarate--CoA ligase-like 5 OS=Cucurbita maxima OX=3661 GN=LOC111476465 PE=4 SV=1)

HSP 1 Score: 967 bits (2500), Expect = 0.0
Identity = 492/552 (89.13%), Postives = 521/552 (94.38%), Query Frame = 0


Query: 1   MALYGGGDNPAVDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDA 60
           MA+YGG D P VDPRSGFC STKIFHSKRRPIPLPP QSL+AT+FISSRPHNGKIALIDA
Sbjct: 1   MAVYGG-DVPQVDPRSGFCKSTKIFHSKRRPIPLPPNQSLDATTFISSRPHNGKIALIDA 60

Query: 61  ATGQHITYSQLWEFVDSVAAALSDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTT 120
            TGQHITYS LWE VDSVA++LSD+GIRKGHVILLLSPNSIFFPIICLAVMS+GA+ITTT
Sbjct: 61  TTGQHITYSHLWESVDSVASSLSDMGIRKGHVILLLSPNSIFFPIICLAVMSLGAVITTT 120

Query: 121 NPLNTAQEIAKQIADSKPILAFTTQQLIPKIASSNLPIVLIDGQNENPAGNKVKIVSNLG 180
           NPLNT QEIAKQIADS PILAFTTQQLIPKIASS LP+VLIDG+ +    + VKIVS L 
Sbjct: 121 NPLNTPQEIAKQIADSNPILAFTTQQLIPKIASSKLPVVLIDGEIQIKKFS-VKIVSTLS 180

Query: 181 EMMRKNPSGSRITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDG 240
           EMMRK  SGSR+ E V+QNDTATLLYSSGTTGASKGVVSSHKN+IAMVQVVVTRF LS+G
Sbjct: 181 EMMRKKSSGSRVKERVDQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFKLSEG 240

Query: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPIL 300
           EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIE+YRAT+LPLVPPIL
Sbjct: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIEKYRATYLPLVPPIL 300

Query: 301 VALVNAADQIKGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGA 360
           VA+VNAA+QIKGKYDL SL TALSGGAPLGKEVIEGFVEKYPNV+ILQGYGLTESTGIGA
Sbjct: 301 VAMVNAAEQIKGKYDLGSLHTALSGGAPLGKEVIEGFVEKYPNVSILQGYGLTESTGIGA 360

Query: 361 STDSLEESRRYGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEAT 420
           STDSLEESRRYGTAGLLSPSTE KIV PD+GEALPV  TGELWLRGPTIMKGYFGNVEAT
Sbjct: 361 STDSLEESRRYGTAGLLSPSTEAKIVVPDTGEALPVNTTGELWLRGPTIMKGYFGNVEAT 420

Query: 421 TSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAA 480
           TSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHP+ISDAA
Sbjct: 421 TSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPSISDAA 480

Query: 481 VIPYPDRDVGQYPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGK 540
           VIPYPD++VGQYPMAYVVRKGG+DISHED+MQ +A+QVAPYKRIRRVAFVDSIPKNPSGK
Sbjct: 481 VIPYPDKEVGQYPMAYVVRKGGSDISHEDIMQLVARQVAPYKRIRRVAFVDSIPKNPSGK 540

Query: 541 ILRKDLIKLATS 552
           ILRKDLIKLATS
Sbjct: 541 ILRKDLIKLATS 550

[Close]

BLAST of MC11g0065 vs. ExPASy TrEMBL
Match: A0A6J1FMD2 (4-coumarate--CoA ligase-like 5 OS=Cucurbita moschata OX=3662 GN=LOC111446530 PE=4 SV=1)

HSP 1 Score: 961 bits (2484), Expect = 0.0
Identity = 490/552 (88.77%), Postives = 517/552 (93.66%), Query Frame = 0


Query: 1   MALYGGGDNPAVDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDA 60
           MA +GG     +DPRSGFC STKIFHSKR PIPLPP QSL+AT+FISSRPHNGKIALIDA
Sbjct: 1   MAEHGG----EIDPRSGFCKSTKIFHSKRHPIPLPPNQSLDATTFISSRPHNGKIALIDA 60

Query: 61  ATGQHITYSQLWEFVDSVAAALSDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTT 120
            TGQHITYS LWE VDSVA+ALSD+GIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTT
Sbjct: 61  TTGQHITYSHLWESVDSVASALSDMGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTT 120

Query: 121 NPLNTAQEIAKQIADSKPILAFTTQQLIPKIASSNLPIVLIDGQNENPAGNKVKIVSNLG 180
           NPLNT QEIAKQIADSKPILAFTTQQL+PKIASS LPIVLIDG+ + P+   VKIVS L 
Sbjct: 121 NPLNTPQEIAKQIADSKPILAFTTQQLLPKIASSKLPIVLIDGEIQTPS---VKIVSTLN 180

Query: 181 EMMRKNPSGSRITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDG 240
           EMMRK PS SRI E VEQNDTATLLYSSGTTGASKGVVSSHKN+IAMVQVVVTRF LS+G
Sbjct: 181 EMMRKKPSESRIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFRLSEG 240

Query: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPIL 300
           EGTFICTVPMFHIYGLVAFATGLLSSGSTI+VLSKFEIHEMLSAI++YRAT+LPLVPPIL
Sbjct: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIIVLSKFEIHEMLSAIDKYRATYLPLVPPIL 300

Query: 301 VALVNAADQIKGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGA 360
           VALVNAA+QIKGKYDLSSL TALSGGAPLGKEVIEGFVEKYPNV ILQGYGLTESTGIGA
Sbjct: 301 VALVNAAEQIKGKYDLSSLHTALSGGAPLGKEVIEGFVEKYPNVAILQGYGLTESTGIGA 360

Query: 361 STDSLEESRRYGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEAT 420
           STDSLEESRRYGTAGLLSPSTE  IVDP++G+ LPV RTGELWLRGPT+MKGYFGNVEAT
Sbjct: 361 STDSLEESRRYGTAGLLSPSTEAMIVDPETGKPLPVNRTGELWLRGPTVMKGYFGNVEAT 420

Query: 421 TSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAA 480
            STLDS+GWLRTGDLCYID+DGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAA
Sbjct: 421 ASTLDSKGWLRTGDLCYIDQDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAA 480

Query: 481 VIPYPDRDVGQYPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGK 540
           VIP+PD++VGQYPMAYVV KGG+DISHEDVMQF AKQVAPYKRIRRVAFVDSIPKNPSGK
Sbjct: 481 VIPFPDKEVGQYPMAYVVGKGGSDISHEDVMQFAAKQVAPYKRIRRVAFVDSIPKNPSGK 540

Query: 541 ILRKDLIKLATS 552
           ILRKDLIKLATS
Sbjct: 541 ILRKDLIKLATS 545

[Close]

BLAST of MC11g0065 vs. ExPASy TrEMBL
Match: A0A0A0L385 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G653450 PE=4 SV=1)

HSP 1 Score: 951 bits (2457), Expect = 0.0
Identity = 485/552 (87.86%), Postives = 516/552 (93.48%), Query Frame = 0


Query: 1   MALYGGGDNPAVDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDA 60
           MA YGG +N  VDPRSGFC STKIF+SKRRPIPLPP QSL+AT+FISSRPHNGKIALIDA
Sbjct: 1   MAEYGG-ENLEVDPRSGFCKSTKIFNSKRRPIPLPPNQSLDATTFISSRPHNGKIALIDA 60

Query: 61  ATGQHITYSQLWEFVDSVAAALSDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTT 120
           +TG HITYS LW  V SVA++LSD+GIRKGHVILLLSPNSI FPIICLAVMSIGAIITTT
Sbjct: 61  STGHHITYSDLWNAVHSVASSLSDMGIRKGHVILLLSPNSIHFPIICLAVMSIGAIITTT 120

Query: 121 NPLNTAQEIAKQIADSKPILAFTTQQLIPKIASSNLPIVLIDGQNENPAGNKVKIVSNLG 180
           NPLNT QEI+KQI+DSKPILAFTTQ LIPKIA+S LP+V+IDGQ      ++ KIVS L 
Sbjct: 121 NPLNTPQEISKQISDSKPILAFTTQALIPKIATSKLPVVVIDGQIPK---SQAKIVSTLS 180

Query: 181 EMMRKNPSGSRITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDG 240
           EMMRK PSGS+I E VEQNDTATLLYSSGTTGASKGVVSSHKN+IAMVQVVVTRF LS+G
Sbjct: 181 EMMRKKPSGSQIKERVEQNDTATLLYSSGTTGASKGVVSSHKNLIAMVQVVVTRFKLSEG 240

Query: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPIL 300
           EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIE+Y+AT+LPLVPPIL
Sbjct: 241 EGTFICTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIEKYKATYLPLVPPIL 300

Query: 301 VALVNAADQIKGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGA 360
           VALVNAA+QIKGKYDL SL TALSGGAPLGKEVIEGFVEK+PNV ILQGYGLTESTGIGA
Sbjct: 301 VALVNAAEQIKGKYDLGSLHTALSGGAPLGKEVIEGFVEKFPNVAILQGYGLTESTGIGA 360

Query: 361 STDSLEESRRYGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEAT 420
           STDSLEESRRYGTAGLLSPSTEG IVDP++GEALPV RTGELWLRGPT+MKGYFGNVEAT
Sbjct: 361 STDSLEESRRYGTAGLLSPSTEGMIVDPETGEALPVNRTGELWLRGPTVMKGYFGNVEAT 420

Query: 421 TSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAA 480
           +STLDS GWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAA
Sbjct: 421 SSTLDSAGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAA 480

Query: 481 VIPYPDRDVGQYPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGK 540
           VIPYPD+DVGQ+PMAYVVRK G+DISH DVMQF+AKQVAPYKRIRRVAFVDSIPKNPSGK
Sbjct: 481 VIPYPDKDVGQFPMAYVVRKVGSDISHNDVMQFVAKQVAPYKRIRRVAFVDSIPKNPSGK 540

Query: 541 ILRKDLIKLATS 552
           ILRKDLIKLATS
Sbjct: 541 ILRKDLIKLATS 548

[Close]

BLAST of MC11g0065 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 809.3 bits (2089), Expect = 1.9e-234
Identity = 403/548 (73.54%), Postives = 471/548 (85.95%), Query Frame = 0


Query: 11  AVDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDAATGQHITYSQ 70
           +V+ RSGFC+S   F+SKR PIPLPP  SL+ T+FISS+ H G+IA IDA+TGQ++T+++
Sbjct: 3   SVNSRSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFISSQAHRGRIAFIDASTGQNLTFTE 62

Query: 71  LWEFVDSVAAALSDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTAQEIA 130
           LW  V+SVA  LS+IGIRKGHV+LLLSPNSI FP++CL+VMS+GAIITTTNPLNT+ EIA
Sbjct: 63  LWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIA 122

Query: 131 KQIADSKPILAFTTQQLIPKI--ASSNLPIVLIDGQNENPAGNKVKIVSNLGEMMRKNPS 190
           KQI DS P+LAFTT QL+PKI  A+  LPIVL+D +  +  G+    V  L EMM+K PS
Sbjct: 123 KQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGD----VRRLVEMMKKEPS 182

Query: 191 GSRITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDGEGTFICTV 250
           G+R+ E V+Q+DTATLLYSSGTTG SKGV+SSH+N+IAMVQ +V RF   DGE  FICTV
Sbjct: 183 GNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFICTV 242

Query: 251 PMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPILVALVNAAD 310
           PMFHIYGL AFATGLL+ GSTI+VLSKFE+HEM+SAI +Y+AT LPLVPPILVA+VN AD
Sbjct: 243 PMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGAD 302

Query: 311 QIKGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGASTDSLEES 370
           QIK KYDLSS+ T L GGAPL KEV EGF EKYP V ILQGYGLTESTGIGASTD++EES
Sbjct: 303 QIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEES 362

Query: 371 RRYGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEATTSTLDSEG 430
           RRYGTAG LS S EG+IVDP +G+ L  K+TGELWL+GP+IMKGYF N EAT+STLDSEG
Sbjct: 363 RRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEG 422

Query: 431 WLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAAVIPYPDRD 490
           WLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQV PAELEALLLTHP I+DAAVIP+PD++
Sbjct: 423 WLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKE 482

Query: 491 VGQYPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIK 550
           VGQ+PMAYVVRK G+ +S + +M+F+AKQVAPYKRIR+VAFV SIPKNPSGKILRKDLIK
Sbjct: 483 VGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLIK 542

Query: 551 LATSNSKL 557
           +ATSNSKL
Sbjct: 543 IATSNSKL 546

[Close]

BLAST of MC11g0065 vs. TAIR 10
Match: AT1G20510.2 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 692.2 bits (1785), Expect = 3.4e-199
Identity = 347/475 (73.05%), Postives = 403/475 (84.84%), Query Frame = 0


Query: 11  AVDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDAATGQHITYSQ 70
           +V+ RSGFC+S   F+SKR PIPLPP  SL+ T+FISS+ H G+IA IDA+TGQ++T+++
Sbjct: 3   SVNSRSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFISSQAHRGRIAFIDASTGQNLTFTE 62

Query: 71  LWEFVDSVAAALSDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTAQEIA 130
           LW  V+SVA  LS+IGIRKGHV+LLLSPNSI FP++CL+VMS+GAIITTTNPLNT+ EIA
Sbjct: 63  LWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIA 122

Query: 131 KQIADSKPILAFTTQQLIPKI--ASSNLPIVLIDGQNENPAGNKVKIVSNLGEMMRKNPS 190
           KQI DS P+LAFTT QL+PKI  A+  LPIVL+D +  +  G+    V  L EMM+K PS
Sbjct: 123 KQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGD----VRRLVEMMKKEPS 182

Query: 191 GSRITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDGEGTFICTV 250
           G+R+ E V+Q+DTATLLYSSGTTG SKGV+SSH+N+IAMVQ +V RF   DGE  FICTV
Sbjct: 183 GNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFICTV 242

Query: 251 PMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPILVALVNAAD 310
           PMFHIYGL AFATGLL+ GSTI+VLSKFE+HEM+SAI +Y+AT LPLVPPILVA+VN AD
Sbjct: 243 PMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGAD 302

Query: 311 QIKGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGASTDSLEES 370
           QIK KYDLSS+ T L GGAPL KEV EGF EKYP V ILQGYGLTESTGIGASTD++EES
Sbjct: 303 QIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEES 362

Query: 371 RRYGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEATTSTLDSEG 430
           RRYGTAG LS S EG+IVDP +G+ L  K+TGELWL+GP+IMKGYF N EAT+STLDSEG
Sbjct: 363 RRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEG 422

Query: 431 WLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAAVIP 484
           WLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQV PAELEALLLTHP I+DAAVIP
Sbjct: 423 WLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIP 473

[Close]

BLAST of MC11g0065 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 681.4 bits (1757), Expect = 6.1e-196
Identity = 338/545 (62.02%), Postives = 427/545 (78.35%), Query Frame = 0


Query: 12  VDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDAATGQHITYSQL 71
           VDPRSGFC S   F+SKR+P+ LPP  S + T+FISS+PH GK A IDAATGQ +T+S L
Sbjct: 10  VDPRSGFCKSNSTFYSKRQPLSLPPNLSRDVTTFISSQPHRGKTAFIDAATGQCLTFSDL 69

Query: 72  WEFVDSVAAAL-SDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTAQEIA 131
           W  VD VA  L  ++GIR+G V+L+LSPNSIF P++CL+VMS+GA+ TT N LNT+ EI+
Sbjct: 70  WRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEIS 129

Query: 132 KQIADSKPILAFTTQQLIPKIASSNLPIVLIDGQNENPAGNKVKIVSNLGEMMRKNPSGS 191
           KQIADS P L FTT+QL PK+  + + +VL D +      + +++V  L EM++K PSG 
Sbjct: 130 KQIADSNPTLVFTTRQLAPKLPVA-ISVVLTDDEVYQELTSAIRVVGILSEMVKKEPSGQ 189

Query: 192 RITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDG---EGTFICT 251
           R+ + V Q+DTA +LYSSGTTG SKGV+SSH+N+ A     V RF +SD    +  FICT
Sbjct: 190 RVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAH----VARF-ISDNLKRDDIFICT 249

Query: 252 VPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPILVALVNAA 311
           VPMFH YGL+ FA G ++ GST+V+L +F++H+M+ A+E++RAT L L PP+LVA++N A
Sbjct: 250 VPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPVLVAMINDA 309

Query: 312 DQIKGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGASTDSLEE 371
           D IK KYDLSSL+T   GGAPL KEV EGF+EKYP V ILQGY LTES G GA T+S EE
Sbjct: 310 DLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGGGAFTNSAEE 369

Query: 372 SRRYGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEATTSTLDSE 431
           SRRYGTAG L+   E +IVDP++G  + + +TGELWL+GP+I KGYF N EAT  T++ E
Sbjct: 370 SRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEATNETINLE 429

Query: 432 GWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAAVIPYPDR 491
           GWL+TGDLCYIDEDGF+FVVDRLKELIKYKGYQVPPAELEALL+THP+I DAAVIP+PD+
Sbjct: 430 GWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILDAAVIPFPDK 489

Query: 492 DVGQYPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGKILRKDLI 551
           + GQYPMAYVVRK  +++S + V+ FI+KQVAPYK+IR+V+F++SIPK  SGK LRKDLI
Sbjct: 490 EAGQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFINSIPKTASGKTLRKDLI 548

Query: 552 KLATS 553
           KLATS
Sbjct: 550 KLATS 548

[Close]

BLAST of MC11g0065 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 676.8 bits (1745), Expect = 1.5e-194
Identity = 335/547 (61.24%), Postives = 428/547 (78.24%), Query Frame = 0


Query: 11  AVDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDAATGQHITYSQ 70
           AVD +SGFC ST IF+SKR P+ LPP Q L+ TSFI+S+PH GK   +DA TG+ +++ +
Sbjct: 20  AVDRKSGFCESTSIFYSKREPMALPPNQFLDVTSFIASQPHRGKTVFVDAVTGRRLSFPE 79

Query: 71  LWEFVDSVAAALSDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTAQEIA 130
           LW  V+ VA  L  +G+RKG+V+++LSPNSI FPI+ L+VMS+GAIITT NP+NT+ EI+
Sbjct: 80  LWLGVERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEIS 139

Query: 131 KQIADSKPILAFTTQQLIPKIASS---NLPIVLIDGQN--ENPAGNKVKIVSNLGEMMRK 190
           KQI DS+P+LAFTT +L+ K+A++   NLP+VL+D  +      G++VK+V  L  M+  
Sbjct: 140 KQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIET 199

Query: 191 NPSGSRITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDGEGTFI 250
            PS SR+ + V Q+DTA LLYSSGTTG SKGV+ SH+N+IA+VQ    RF L   E   I
Sbjct: 200 EPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYRARFGL---EQRTI 259

Query: 251 CTVPMFHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPILVALVN 310
           CT+PM HI+G   FATGL++ G TIVVL KF++ ++LSA+E +R+++L LVPPI+VA+VN
Sbjct: 260 CTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMVN 319

Query: 311 AADQIKGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGASTDSL 370
            A++I  KYDLSSL T ++GGAPL +EV E FVE YP V ILQGYGLTEST I AS  + 
Sbjct: 320 GANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFNK 379

Query: 371 EESRRYGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEATTSTLD 430
           EE++RYG +GLL+P+ EGKIVDPD+G  L V +TGELW+R PT+MKGYF N EAT ST+D
Sbjct: 380 EETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTID 439

Query: 431 SEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAAVIPYP 490
           SEGWL+TGDLCYID DGF+FVVDRLKELIK  GYQV PAELEALLL HP I+DAAVIP P
Sbjct: 440 SEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPIP 499

Query: 491 DRDVGQYPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGKILRKD 550
           D   GQYPMAY+VRK G+++S  ++M F+AKQV+PYK+IR+V F+ SIPKNPSGKILR++
Sbjct: 500 DMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKILRRE 559

Query: 551 LIKLATS 553
           L KL TS
Sbjct: 560 LTKLTTS 563

[Close]

BLAST of MC11g0065 vs. TAIR 10
Match: AT5G38120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 650.2 bits (1676), Expect = 1.5e-186
Identity = 324/542 (59.78%), Postives = 412/542 (76.01%), Query Frame = 0


Query: 12  VDPRSGFCSSTKIFHSKRRPIPLPPKQSLNATSFISSRPHNGKIALIDAATGQHITYSQL 71
           +DPR+GFC+S   F+SKR+P+ LP K+SL+ T+FISS+ + GK A IDAAT   I++S L
Sbjct: 11  IDPRNGFCTSNSTFYSKRKPLALPSKESLDITTFISSQTYRGKTAFIDAATDHRISFSDL 70

Query: 72  WEFVDSVA-AALSDIGIRKGHVILLLSPNSIFFPIICLAVMSIGAIITTTNPLNTAQEIA 131
           W  VD VA   L D+GIR+G V+L+LSPN+I  PI+CL+VMS+GA++TT NPLNTA EI 
Sbjct: 71  WMAVDRVADCLLHDVGIRRGDVVLVLSPNTISIPIVCLSVMSLGAVLTTANPLNTASEIL 130

Query: 132 KQIADSKPILAFTTQQLIPKIASSNLPIVLIDGQNENPAGNKVKIVSNLGEMMRKNPSGS 191
           +QIADS P LAFTT +L PKIASS + IVL   ++       +K+V NL EMM+K PSG 
Sbjct: 131 RQIADSNPKLAFTTPELAPKIASSGISIVLERVEDTLRVPRGLKVVGNLTEMMKKEPSGQ 190

Query: 192 RITEPVEQNDTATLLYSSGTTGASKGVVSSHKNIIAMVQVVVTRFNLSDGEGTFICTVPM 251
            +   V ++DTA LLYSSGTTG SKGV SSH N+IA V   +        + TFICTVP+
Sbjct: 191 AVRNQVHKDDTAMLLYSSGTTGRSKGVNSSHGNLIAHVARYIAE-PFEQPQQTFICTVPL 250

Query: 252 FHIYGLVAFATGLLSSGSTIVVLSKFEIHEMLSAIERYRATFLPLVPPILVALVNAADQI 311
           FH +GL+ F    L+ G+T+V+L +F++ EM++A+E+YRAT L LVPP+LV ++N ADQI
Sbjct: 251 FHTFGLLNFVLATLALGTTVVILPRFDLGEMMAAVEKYRATTLILVPPVLVTMINKADQI 310

Query: 312 KGKYDLSSLRTALSGGAPLGKEVIEGFVEKYPNVTILQGYGLTESTGIGASTDSLEESRR 371
             KYD+S LRT   GGAPL KEV +GF++KYP V + QGY LTES G GAS +S+EESRR
Sbjct: 311 MKKYDVSFLRTVRCGGAPLSKEVTQGFMKKYPTVDVYQGYALTESNGAGASIESVEESRR 370

Query: 372 YGTAGLLSPSTEGKIVDPDSGEALPVKRTGELWLRGPTIMKGYFGNVEATTSTLDSEGWL 431
           YG  GLLS   E +IVDP++G+ + + +TGELWL+GP+I KGYF N E     + SEGWL
Sbjct: 371 YGAVGLLSCGVEARIVDPNTGQVMGLNQTGELWLKGPSIAKGYFRNEE---EIITSEGWL 430

Query: 432 RTGDLCYIDEDGFIFVVDRLKELIKYKGYQVPPAELEALLLTHPNISDAAVIPYPDRDVG 491
           +TGDLCYID DGF+F+VDRLKELIKYKGYQVPPAELEALLL HP+I DAAVIP+PD++ G
Sbjct: 431 KTGDLCYIDNDGFLFIVDRLKELIKYKGYQVPPAELEALLLNHPDILDAAVIPFPDKEAG 490

Query: 492 QYPMAYVVRKGGNDISHEDVMQFIAKQVAPYKRIRRVAFVDSIPKNPSGKILRKDLIKLA 551
           Q+PMAYV RK  +++  + V+ FI+KQVAPYK+IR+VAF+DSIPK PSGK LRKDLIK A
Sbjct: 491 QFPMAYVARKPESNLCEKKVIDFISKQVAPYKKIRKVAFIDSIPKTPSGKTLRKDLIKFA 548

Query: 552 TS 553
            S
Sbjct: 551 IS 548

The following BLAST results are available for this feature:

BLAST of MC11g0065 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
M4IQR7	1.5e-239	76.89	Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1	[more]
Q84P21	2.7e-233	73.54	4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=...	[more]
Q10S72	4.7e-201	63.55	4-coumarate--CoA ligase-like 4 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL4...	[more]
P0C5B6	8.5e-195	62.02	OPC-6:CoA ligase OS=Arabidopsis thaliana OX=3702 GN=4CLL4 PE=2 SV=1	[more]
Q84P25	2.1e-193	61.24	4-coumarate--CoA ligase-like 2 OS=Arabidopsis thaliana OX=3702 GN=4CLL2 PE=2 SV=...	[more]

BLAST of MC11g0065 vs. NCBI nr


Match Name	Identity	Description
XP_022936511.1	89.67	4-coumarate--CoA ligase-like 5 [Cucurbita moschata]	[more]
XP_022982315.1	89.49	4-coumarate--CoA ligase-like 5 isoform X1 [Cucurbita maxima]	[more]
XP_023522465.1	89.31	4-coumarate--CoA ligase-like 5 [Cucurbita pepo subsp. pepo]	[more]
XP_022975900.1	89.13	4-coumarate--CoA ligase-like 5 [Cucurbita maxima]	[more]
XP_023534989.1	88.95	4-coumarate--CoA ligase-like 5 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC11g0065 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1F7P0	89.67	4-coumarate--CoA ligase-like 5 OS=Cucurbita moschata OX=3662 GN=LOC111443102 PE=...	[more]
A0A6J1J2B8	89.49	4-coumarate--CoA ligase-like 5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1II10	89.13	4-coumarate--CoA ligase-like 5 OS=Cucurbita maxima OX=3661 GN=LOC111476465 PE=4 ...	[more]
A0A6J1FMD2	88.77	4-coumarate--CoA ligase-like 5 OS=Cucurbita moschata OX=3662 GN=LOC111446530 PE=...	[more]
A0A0A0L385	87.86	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G653450 PE=4 SV=1	[more]

BLAST of MC11g0065 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G20510.1	1.9e-234	73.54	OPC-8:0 CoA ligase1	[more]
AT1G20510.2	3.4e-199	73.05	OPC-8:0 CoA ligase1	[more]
AT1G20500.1	6.1e-196	62.02	AMP-dependent synthetase and ligase family protein	[more]
AT1G20480.1	1.5e-194	61.24	AMP-dependent synthetase and ligase family protein	[more]
AT5G38120.1	1.5e-186	59.78	AMP-dependent synthetase and ligase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 22..451 e-value: 9.3E-126 score: 422.0
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 51..456 e-value: 3.2E-103 score: 345.6
IPR025110	AMP-binding enzyme, C-terminal domain	PFAM	PF13193	AMP-binding_C	coord: 465..540 e-value: 5.0E-17 score: 62.6
None	No IPR available	GENE3D	3.30.300.30		coord: 452..554 e-value: 3.0E-32 score: 113.3
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 12..552
None	No IPR available	PANTHER	PTHR24096:SF339	4-COUMARATE--COA LIGASE-LIKE 3-RELATED	coord: 12..552
None	No IPR available	CDD	cd05904	4CL	coord: 34..546 e-value: 0.0 score: 805.687
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 48..551
IPR020845	AMP-binding, conserved site	PROSITE	PS00455	AMP_BINDING	coord: 204..215

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g0065.1	MC11g0065.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009698	phenylpropanoid metabolic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005777	peroxisome
molecular_function	GO:0016207	4-coumarate-CoA ligase activity