MC10g_new0362 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g_new0362
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionPentatricopeptide repeat-containing protein
LocationMC10: 18038208 .. 18041022 (-)
RNA-Seq ExpressionMC10g_new0362
SyntenyMC10g_new0362
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCCAAAGTTGACCAATACTCATGGAATTCTATGATTTCAGGTTTTGAACAACGTGACCGCTTTGATGAAACTTTAAATTATTTTGCTCGAATGCATAGTCATTGTTTTCTGATGAATGAATATTCATTCGGTAGTGCCCTTAGTGCTTGTGCAAGTTTACGATATTTGAAATTGGGTTCCCAAATCCACAGTTTAATATATAGGTCAAATTATTTATCAGATGCGTATATGGGCTCTGCTTTAATAGATATGTACTTTAAATGTGGGAGAGTTGACTACGCTCAAAGTGTTTTTGATGGAATGACCGTGAAAGTAGAGTTTTTTGGAACAACTTGATTATGTGTTATGAATAGAATGGTCTGGTTGATGAGGCTCTTGTTATTATCATTATTTTTTTGAAATGATCAAATGTAGGGTTGAAGCTGATGAAGTAACATTTGCTAGTGTGGTTAGTGCATGTGCAACTATGTCGACGATCAAAGAAGGTCAGCAGATTCATGCTCGTGTTGTGAAATGTGATGAATTTAGAAATGATCTTATTTTAGGCAATGCATTGGTTGATAGGTATGCCAAATATAACAGGATTAACGAGGCTAGACTAGTTTTCAATCAGATGTCAATTAGGAGTGTGGTGTCTGAAACCTCAATGGTACGTGGCTATGCAAAAGCATCAAGTGTCAAAGCTGCAAGATGTACGTTCTTAAATATGATGGTGAAAAATATAATTACTTAGAATGCGTTAATTGCAGGGTGTACACAAAATGGAGAGAATGAAGAGGCACTTATACTCTTTCGCTTTTGAAGAGGGAGTCTTTGGCTTACACACTACACCTTTGACAATCTCCTCAATGCTGGTGCAAACCTTGCTGATTTGCAGCTTGGCCAATGTTGGTTCGATTTATGTCCCCTTGTGCAAGTGAAAAAGAGATCGTATCCACAGGGATTGATGGTTAAACACTTCAATAATCACTTAAGATGAGGGATTTTAAACAAGCAAAGTATTTATAACAAATAGGAAAACTAAAGTAAATCAACTAAAACAATACACCAATACACTACTGTTAAAATGAATCAAAGACAGATGACAGCAAAATGTTTGAGGAAAATGCGCAAATATGAACTGAAATATTGATATTAAAGTGATTAAGGGGGAAGCGGCTAAGTGACTCTCATGGACTTAAGCGGACAATGCTACAGCATAAACCAGATTTTCTACTGTTAACATTGATAGACAAACAATAACAGCATATATGCACAGTTTTCATGACCAAGCTAGGCTTATTCCTAAGTCACCACAAGCTAAACTCAAGTAGGCTCTAGCTCGAGATGAAGTATTTCGCTCCCTATTACGATTTTCCAAAAGTTAACAAGAATGTATTCTAACATGCTGATCAAACATATTGAAAATAAATTTATAAAACAACTTCAACTCAATGTTCTTCATCATTTAAAAACAGTAACAAAGTCTACAGCCATAAGGGTTCACAACAACCTCCCTTTCACAAATTAGATTAGCTACGCAAAGCCATATAAGAAAATCAAATAAAAACTAGAATGAAAATAAAACAAAGAGGTTCAAATAAGACAAAGGATAAGTGGGCCCCATCTCTAAATTTCAGATATTTCTCCGAGTTACTGTGGTAAATCCATTGAGCAGTCCTTTCACTATCAATTTGTCGTTTTCTACTGCTTTATAAATCTGGATCTGATGTTGTAGTATCGTCAAAGCTGTAGCAAAATGCACCAAAATTAGCTCAATTGCCATTGACAATACTAAAATGTAGAAAACTAGTGTTTATGATCTTAGCACAAACTAAGTGAATAAGGGTGCAAGTGATTCTATCTTTGTCTAATTTATACCAACATTATTCCCAAGTAACTCTCTATTTGGAGGGTTATCGGCCGACATGCTCACTCTCATGTTTTAAAGCATGGATTTCGATTTCGATTTCAATCAAGAGAAGAGTCGGATATTTTTGTTGGGAATTCTCTAATTGATATGTATATGAAATGTAGATCAGTTGAGAATGGTGGTAAGGTGTTTGAACATATGGTGCAAAGGGATTGTGTCTCATGGAATGCTATGATAATTGGATATGCACAAAATAGCTTTGGTAATGAGGCTCTTGGAGTTTTCAATCAAATGTTAGAATTAGGAGAGAAACCAGATCATGTCATGATGATTAGTATTTTTTCCGCTTGTAGTCATGTTGGGTTGCTTGATGAAGGCCGCCATTACTTTCAATCCATGAGTGCACAACATGGTTTGGTGTCATTAAAAGACCATCATACATGTATGGTTGATTTACTAGGCCAAGCTGGCTGCCCTGGAGAAGCAAAAGATCTAATAAAGGAAATCTTGATGTAGCCTGATGCTATCATATGGGGATCCTTGCTTGCTGCTTGTAAAGTCCATCGGAATATCAAATTGGGGGAATATGTAGTACAGAAGCTTTTGGAGGTAGATGCTGAGAATTCTGGGCGATATGTTCTTCTCTCGAATATGTATGCTGAACGTGGAGATTGGGGGAATGTTGTGAGAATAAGAAAGTTGATGAGAAAGAGAGGAGTGGTTAAACATCCAAGTTGCAGTTGGATTGAAATTCAAGGTCAGTTGGATATTTTTATGGTTAAGGATAAAAGACATCCAAAGAAGAAAGAAATCTACATGCTTTTGAGAACATTTCTACAACAGATGAGACGAGCTGGATATGTCCCATATGTTTGCAGCAATGAAATTGATGAAGAACAATCAAAAAACGTGACAATGTCCTCGTCTTACCAAATTGAAATGTCACGTACAGGTGAATAG

mRNA sequence

ATGCCCAAAGTTGACCAATACTCATGGAATTCTATGATTTCAGGTTTTGAACAACGTGACCGCTTTGATGAAACTTTAAATTATTTTGCTCGAATGCATAGTCATTGTTTTCTGATGAATGAATATTCATTCGGTAGTGCCCTTAGTGCTTGTGCAAGTTTACGATATTTGAAATTGGGTTCCCAAATCCACAGTTTAATATATAGGGTTGAAGCTGATGAAGTAACATTTGCTAGTGTGGTTAGTGCATGTGCAACTATGTCGACGATCAAAGAAGGTCAGCAGATTCATGCTCGTGTTGTGAAATGTGATGAATTTAGAAATGATCTTATTTTAGGCAATGCATTGGTTGATAGGTATGCCAAATATAACAGGATTAACGAGGCTAGACTAGTTTTCAATCAGATGTCAATTAGGAGTGTGGTGTCTGAAACCTCAATGGTACGTGGCTATGCAAAAGCATCAAGTGTCAAAGCTGCAAGATCTTGGCCAATGTTGGTTCGATTTATGTCCCCTTGTGCAAGTGAAAAAGAGATCCCTGATGCTATCATATGGGGATCCTTGCTTGCTGCTTGTAAAGTCCATCGGAATATCAAATTGGGGGAATATGTAGTACAGAAGCTTTTGGAGGTAGATGCTGAGAATTCTGGGCGATATGTTCTTCTCTCGAATATGTATGCTGAACGTGGAGATTGGGGGAATGTTGTGAGAATAAGAAAGTTGATGAGAAAGAGAGGAGTGGTTAAACATCCAAGTTGCAGTTGGATTGAAATTCAAGGTCAGTTGGATATTTTTATGGTTAAGGATAAAAGACATCCAAAGAAGAAAGAAATCTACATGCTTTTGAGAACATTTCTACAACAGATGAGACGAGCTGGATATGTCCCATATGTTTGCAGCAATGAAATTGATGAAGAACAATCAAAAAACGTGACAATGTCCTCGTCTTACCAAATTGAAATGTCACGTACAGGTGAATAG

Coding sequence (CDS)

ATGCCCAAAGTTGACCAATACTCATGGAATTCTATGATTTCAGGTTTTGAACAACGTGACCGCTTTGATGAAACTTTAAATTATTTTGCTCGAATGCATAGTCATTGTTTTCTGATGAATGAATATTCATTCGGTAGTGCCCTTAGTGCTTGTGCAAGTTTACGATATTTGAAATTGGGTTCCCAAATCCACAGTTTAATATATAGGGTTGAAGCTGATGAAGTAACATTTGCTAGTGTGGTTAGTGCATGTGCAACTATGTCGACGATCAAAGAAGGTCAGCAGATTCATGCTCGTGTTGTGAAATGTGATGAATTTAGAAATGATCTTATTTTAGGCAATGCATTGGTTGATAGGTATGCCAAATATAACAGGATTAACGAGGCTAGACTAGTTTTCAATCAGATGTCAATTAGGAGTGTGGTGTCTGAAACCTCAATGGTACGTGGCTATGCAAAAGCATCAAGTGTCAAAGCTGCAAGATCTTGGCCAATGTTGGTTCGATTTATGTCCCCTTGTGCAAGTGAAAAAGAGATCCCTGATGCTATCATATGGGGATCCTTGCTTGCTGCTTGTAAAGTCCATCGGAATATCAAATTGGGGGAATATGTAGTACAGAAGCTTTTGGAGGTAGATGCTGAGAATTCTGGGCGATATGTTCTTCTCTCGAATATGTATGCTGAACGTGGAGATTGGGGGAATGTTGTGAGAATAAGAAAGTTGATGAGAAAGAGAGGAGTGGTTAAACATCCAAGTTGCAGTTGGATTGAAATTCAAGGTCAGTTGGATATTTTTATGGTTAAGGATAAAAGACATCCAAAGAAGAAAGAAATCTACATGCTTTTGAGAACATTTCTACAACAGATGAGACGAGCTGGATATGTCCCATATGTTTGCAGCAATGAAATTGATGAAGAACAATCAAAAAACGTGACAATGTCCTCGTCTTACCAAATTGAAATGTCACGTACAGGTGAATAG

Protein sequence

MPKVDQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLGSQIHSLIYRVEADEVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLILGNALVDRYAKYNRINEARLVFNQMSIRSVVSETSMVRGYAKASSVKAARSWPMLVRFMSPCASEKEIPDAIIWGSLLAACKVHRNIKLGEYVVQKLLEVDAENSGRYVLLSNMYAERGDWGNVVRIRKLMRKRGVVKHPSCSWIEIQGQLDIFMVKDKRHPKKKEIYMLLRTFLQQMRRAGYVPYVCSNEIDEEQSKNVTMSSSYQIEMSRTGE
Homology
BLAST of MC10g_new0362 vs. ExPASy Swiss-Prot
Match: Q9SIT7 (Pentatricopeptide repeat-containing protein At2g13600 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E76 PE=3 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.4e-64
Identity = 184/584 (31.51%), Postives = 229/584 (39.21%), Query Frame = 0

Query: 1   MPKVDQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLG 60
           MP+ DQ +WNSM+SGF Q DR +E L YFA MH   F++NEYSF S LSAC+ L  +  G
Sbjct: 112 MPERDQCTWNSMVSGFAQHDRCEEALCYFAMMHKEGFVLNEYSFASVLSACSGLNDMNKG 171

Query: 61  SQIHSLI----------------------------------------------------- 120
            Q+HSLI                                                     
Sbjct: 172 VQVHSLIAKSPFLSDVYIGSALVDMYSKCGNVNDAQRVFDEMGDRNVVSWNSLITCFEQN 231

Query: 121 ---------------YRVEADEVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLIL 180
                           RVE DEVT ASV+SACA++S IK GQ++H RVVK D+ RND+IL
Sbjct: 232 GPAVEALDVFQMMLESRVEPDEVTLASVISACASLSAIKVGQEVHGRVVKNDKLRNDIIL 291

Query: 181 GNALVDRYAKYNRINEARLVFNQMSIRSVVSETSMVRGYAKASSVKAAR----------- 240
            NA VD YAK +RI EAR +F+ M IR+V++ETSM+ GYA A+S KAAR           
Sbjct: 292 SNAFVDMYAKCSRIKEARFIFDSMPIRNVIAETSMISGYAMAASTKAARLMFTKMAERNV 351

Query: 241 -SWPMLV--------------------------------RFMSPCASEKEI--------- 300
            SW  L+                                  +  CA   E+         
Sbjct: 352 VSWNALIAGYTQNGENEEALSLFCLLKRESVCPTHYSFANILKACADLAELHLGMQAHVH 411

Query: 301 ------------------------------------------------------------ 317
                                                                       
Sbjct: 412 VLKHGFKFQSGEEDDIFVGNSLIDMYVKCGCVEEGYLVFRKMMERDCVSWNAMIIGFAQN 471

BLAST of MC10g_new0362 vs. ExPASy Swiss-Prot
Match: Q9SI53 (Pentatricopeptide repeat-containing protein At2g03880, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-H44 PE=2 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.3e-37
Identity = 107/356 (30.06%), Postives = 155/356 (43.54%), Query Frame = 0

Query: 5   DQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLGSQIH 64
           D   WNS+I GF Q  R D  L  F RM    F+                          
Sbjct: 224 DAIVWNSIIGGFAQNSRSDVALELFKRMKRAGFI-------------------------- 283

Query: 65  SLIYRVEADEVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLILGNALVDRYAKYN 124
                  A++ T  SV+ AC  ++ ++ G Q H  +VK D+   DLIL NALVD Y K  
Sbjct: 284 -------AEQATLTSVLRACTGLALLELGMQAHVHIVKYDQ---DLILNNALVDMYCKCG 343

Query: 125 RINEARLVFNQMSIRSVVSETSMVRGYA---------------KASSVKAARSWPMLVRF 184
            + +A  VFNQM  R V++ ++M+ G A               K+S  K   ++  +V  
Sbjct: 344 SLEDALRVFNQMKERDVITWSTMISGLAQNGYSQEALKLFERMKSSGTKP--NYITIVGV 403

Query: 185 MSPCA-------------SEKEI------------------------------------P 244
           +  C+             S K++                                    P
Sbjct: 404 LFACSHAGLLEDGWYYFRSMKKLYGIDPVREHYGCMIDLLGKAGKLDDAVKLLNEMECEP 463

Query: 245 DAIIWGSLLAACKVHRNIKLGEYVVQKLLEVDAENSGRYVLLSNMYAERGDWGNVVRIRK 297
           DA+ W +LL AC+V RN+ L EY  +K++ +D E++G Y LLSN+YA    W +V  IR 
Sbjct: 464 DAVTWRTLLGACRVQRNMVLAEYAAKKVIALDPEDAGTYTLLSNIYANSQKWDSVEEIRT 523

BLAST of MC10g_new0362 vs. ExPASy Swiss-Prot
Match: Q9SY02 (Pentatricopeptide repeat-containing protein At4g02750 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H24 PE=3 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 4.3e-37
Identity = 113/386 (29.27%), Postives = 170/386 (44.04%), Query Frame = 0

Query: 1   MPKVDQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLG 60
           MPK D  SW +MI+G+ Q     E L  F +M      +N  SF SALS CA +  L+L 
Sbjct: 369 MPKRDPVSWAAMIAGYSQSGHSFEALRLFVQMEREGGRLNRSSFSSALSTCADVVALEL- 428

Query: 61  SQIHSLIYRVEADEVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLILGNALVDRY 120
                                           G+Q+H R+VK   +     +GNAL+  Y
Sbjct: 429 --------------------------------GKQLHGRLVK-GGYETGCFVGNALLLMY 488

Query: 121 AKYNRINEARLVFNQMSIRSVVSETSMVRGYAKASSVKAARSW-------------PMLV 180
            K   I EA  +F +M+ + +VS  +M+ GY++    + A  +               +V
Sbjct: 489 CKCGSIEEANDLFKEMAGKDIVSWNTMIAGYSRHGFGEVALRFFESMKREGLKPDDATMV 548

Query: 181 RFMSPCASEKEI------------------------------------------------ 240
             +S C+    +                                                
Sbjct: 549 AVLSACSHTGLVDKGRQYFYTMTQDYGVMPNSQHYACMVDLLGRAGLLEDAHNLMKNMPF 608

Query: 241 -PDAIIWGSLLAACKVHRNIKLGEYVVQKLLEVDAENSGRYVLLSNMYAERGDWGNVVRI 300
            PDA IWG+LL A +VH N +L E    K+  ++ ENSG YVLLSN+YA  G WG+V ++
Sbjct: 609 EPDAAIWGTLLGASRVHGNTELAETAADKIFAMEPENSGMYVLLSNLYASSGRWGDVGKL 668

Query: 301 RKLMRKRGVVKHPSCSWIEIQGQLDIFMVKDKRHPKKKEIYMLLRTFLQQMRRAGYV--P 323
           R  MR +GV K P  SWIEIQ +   F V D+ HP+K EI+  L     +M++AGYV   
Sbjct: 669 RVRMRDKGVKKVPGYSWIEIQNKTHTFSVGDEFHPEKDEIFAFLEELDLRMKKAGYVSKT 720

BLAST of MC10g_new0362 vs. ExPASy Swiss-Prot
Match: Q9LW63 (Putative pentatricopeptide repeat-containing protein At3g23330 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H32 PE=3 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 2.1e-36
Identity = 100/382 (26.18%), Postives = 173/382 (45.29%), Query Frame = 0

Query: 5   DQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLGSQIH 64
           D  SWNS+++G+ Q  R++E L  F +M                                
Sbjct: 307 DGISWNSLVAGYVQNGRYNEALRLFRQM-------------------------------- 366

Query: 65  SLIYRVEADEVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLILGNALVDRYAKYN 124
            +  +V+   V F+SV+ ACA ++T+  G+Q+H  V++   F +++ + +ALVD Y+K  
Sbjct: 367 -VTAKVKPGAVAFSSVIPACAHLATLHLGKQLHGYVLR-GGFGSNIFIASALVDMYSKCG 426

Query: 125 RINEARLVFNQMSIRSVVSETSMVRGYA---------------KASSVK----------- 184
            I  AR +F++M++   VS T+++ G+A               K   VK           
Sbjct: 427 NIKAARKIFDRMNVLDEVSWTAIIMGHALHGHGHEAVSLFEEMKRQGVKPNQVAFVAVLT 486

Query: 185 -------AARSWPMLVRFMSPCASEKEI-----------------------------PDA 244
                     +W             +E+                             P  
Sbjct: 487 ACSHVGLVDEAWGYFNSMTKVYGLNQELEHYAAVADLLGRAGKLEEAYNFISKMCVEPTG 546

Query: 245 IIWGSLLAACKVHRNIKLGEYVVQKLLEVDAENSGRYVLLSNMYAERGDWGNVVRIRKLM 304
            +W +LL++C VH+N++L E V +K+  VD+EN G YVL+ NMYA  G W  + ++R  M
Sbjct: 547 SVWSTLLSSCSVHKNLELAEKVAEKIFTVDSENMGAYVLMCNMYASNGRWKEMAKLRLRM 606

Query: 305 RKRGVVKHPSCSWIEIQGQLDIFMVKDKRHPKKKEIYMLLRTFLQQMRRAGYVPYVCS-- 323
           RK+G+ K P+CSWIE++ +   F+  D+ HP   +I   L+  ++QM + GYV       
Sbjct: 607 RKKGLRKKPACSWIEMKNKTHGFVSGDRSHPSMDKINEFLKAVMEQMEKEGYVADTSGVL 654

BLAST of MC10g_new0362 vs. ExPASy Swiss-Prot
Match: Q9LIQ7 (Pentatricopeptide repeat-containing protein At3g24000, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-H87 PE=3 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.5e-34
Identity = 107/425 (25.18%), Postives = 179/425 (42.12%), Query Frame = 0

Query: 1   MPKVDQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLG 60
           MP+ D  +W ++ISG+ Q DR  + L +F +M    +  NE++  S + A A+ R    G
Sbjct: 121 MPQRDFVTWTTLISGYSQHDRPCDALLFFNQMLRFGYSPNEFTLSSVIKAAAAERRGCCG 180

Query: 61  SQIHSLIYRVEAD----------------------------------------------- 120
            Q+H    +   D                                               
Sbjct: 181 HQLHGFCVKCGFDSNVHVGSALLDLYTRYGLMDDAQLVFDALESRNDVSWNALIAGHARR 240

Query: 121 ---------------------EVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLIL 180
                                  ++AS+  AC++   +++G+ +HA ++K  E +     
Sbjct: 241 SGTEKALELFQGMLRDGFRPSHFSYASLFGACSSTGFLEQGKWVHAYMIKSGE-KLVAFA 300

Query: 181 GNALVDRYAKYNRINEARLVFNQMSIRSVVSETSMVRGYAKASSVKAARSW--------- 240
           GN L+D YAK   I++AR +F++++ R VVS  S++  YA+    K A  W         
Sbjct: 301 GNTLLDMYAKSGSIHDARKIFDRLAKRDVVSWNSLLTAYAQHGFGKEAVWWFEEMRRVGI 360

Query: 241 -PMLVRFMS---PCA--------------------------------------------- 297
            P  + F+S    C+                                             
Sbjct: 361 RPNEISFLSVLTACSHSGLLDEGWHYYELMKKDGIVPEAWHYVTVVDLLGRAGDLNRALR 420

BLAST of MC10g_new0362 vs. NCBI nr
Match: XP_022134814.1 (pentatricopeptide repeat-containing protein At2g13600-like [Momordica charantia])

HSP 1 Score: 416 bits (1068), Expect = 5.70e-139
Identity = 257/457 (56.24%), Postives = 272/457 (59.52%), Query Frame = 0

Query: 1   MPKVDQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLG 60
           MP+VDQ SWNSMISGFEQ D FDE LNYFA+MHSH F MNEYSFGSALSACA LR LKLG
Sbjct: 111 MPEVDQCSWNSMISGFEQHDCFDEALNYFAQMHSHGFQMNEYSFGSALSACAGLRDLKLG 170

Query: 61  SQIHSLIYR--------------------------------------------------- 120
           SQIHSLIYR                                                   
Sbjct: 171 SQIHSLIYRSNYISDVYMGSALVDMYSKCGRVDRARSVFDGMTVRSRVSWNSLITCYEQN 230

Query: 121 -----------------VEADEVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLIL 180
                            VEADEVT ASVVSACAT+S IK GQQIH RVVKCDEFRNDLIL
Sbjct: 231 GPVDEALVIFFEMIKCRVEADEVTLASVVSACATISAIK-GQQIHTRVVKCDEFRNDLIL 290

Query: 181 GNALVDRYAKYNRINEARLVFNQMSIRSVVSETSM--------------------VRGYA 240
            NALVD YAK NRINEAR+VF+ M IRSVVSETSM                    + GYA
Sbjct: 291 CNALVDMYAKCNRINEARIVFDHMPIRSVVSETSMGVHKMERMKRHLNCVSWNAMIVGYA 350

Query: 241 KAS-------------------------SVKAARSWPMLV-------RFMSP-------- 300
           +                            V  A S   L+       R MS         
Sbjct: 351 QNGFGNEALGVFSKMLESGEKPDHVTMIGVLCACSHAGLLDEGRHYFRSMSAQYGLVSLK 410

Query: 301 --------------CASE-KEI-------PDAIIWGSLLAACKVHRNIKLGEYVVQKLLE 307
                         C  E K+I       PDAIIWGSLLAACKVHRNIKLGEYVV+KLLE
Sbjct: 411 DHYTCMVDLLGRAGCLEEAKDIIEEMPMQPDAIIWGSLLAACKVHRNIKLGEYVVEKLLE 470

BLAST of MC10g_new0362 vs. NCBI nr
Match: XP_022159229.1 (pentatricopeptide repeat-containing protein At2g13600-like [Momordica charantia])

HSP 1 Score: 405 bits (1042), Expect = 1.60e-133
Identity = 262/577 (45.41%), Postives = 280/577 (48.53%), Query Frame = 0

Query: 1   MPKVDQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLG 60
           MP+VDQ SWNSMISGFEQ DRFDE LNYFA+MHSH FLMNEYSFGSALSACA LR  KLG
Sbjct: 111 MPEVDQCSWNSMISGFEQHDRFDEALNYFAQMHSHGFLMNEYSFGSALSACAGLRDFKLG 170

Query: 61  SQIHSLIYR--------------------------------------------------- 120
           SQIHSLIYR                                                   
Sbjct: 171 SQIHSLIYRSNYLSDVYMGSALVDMYSKCGRVDCARSVFDGMTVRSRVSWNSLITCYEQN 230

Query: 121 -----------------VEADEVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLIL 180
                            VEADEVT ASVVSACAT+S I EGQQIHARVVKCDEFRNDLIL
Sbjct: 231 GPVDEALVIFFEMIKCRVEADEVTLASVVSACATISAINEGQQIHARVVKCDEFRNDLIL 290

Query: 181 GNALVDRYAKYNRINEARLVFNQMSIRSVVSETSMVRGYAKASSVKAARS---------- 240
           GNALVD YAK NRIN+AR+VF++M IRSVVSETSMV GYAKASSVKAAR           
Sbjct: 291 GNALVDMYAKCNRINKARIVFDRMPIRSVVSETSMVSGYAKASSVKAARCMFSNMMVKDV 350

Query: 241 -------------------------------WPMLVRF---MSPCAS------------- 300
                                          WP    F   ++ CA+             
Sbjct: 351 ITWNALIAGCTQNGENEEALILFRLLKRESVWPTHYTFGNLLNACANLADLQLGRQAHSH 410

Query: 301 ------------EKEI-------------------------------------------- 309
                       E +I                                            
Sbjct: 411 VLKHGFRFQSGEESDIFVGNSLIDMYMKCGSVENGCKVFEHMVQRDCVSWNAMIVGYAQN 470

BLAST of MC10g_new0362 vs. NCBI nr
Match: XP_022923215.1 (pentatricopeptide repeat-containing protein At2g13600 [Cucurbita moschata])

HSP 1 Score: 390 bits (1002), Expect = 1.23e-127
Identity = 253/575 (44.00%), Postives = 277/575 (48.17%), Query Frame = 0

Query: 1   MPKVDQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLG 60
           MP+VDQ SWNSMISGFEQ DRFDE L YF +MH H F MNEYSFGSALSACA L+ LK+G
Sbjct: 113 MPQVDQCSWNSMISGFEQHDRFDEALKYFVQMHGHGFFMNEYSFGSALSACAGLQDLKMG 172

Query: 61  SQIHSLIYR--------------------------------------------------- 120
           SQIHSLIYR                                                   
Sbjct: 173 SQIHSLIYRSNYLSDMYMGSALVDMYSKCGRVDCARSVFDGMTVRSRVSWNSLITCYEQN 232

Query: 121 -----------------VEADEVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLIL 180
                            VE DEVT ASVVSACAT+S IKEGQQIHARVVKCDEFRNDLIL
Sbjct: 233 GPVDEALSIFVEMIECGVEPDEVTLASVVSACATVSAIKEGQQIHARVVKCDEFRNDLIL 292

Query: 181 GNALVDRYAKYNRINEARLVFNQMSIRSVVSETSMVRGYAKASSVKAARS---------- 240
           GNAL+D YAK NRINEAR+VF++M IRSVVSETSMV GYAKASSVKAARS          
Sbjct: 293 GNALLDMYAKCNRINEARIVFDRMPIRSVVSETSMVSGYAKASSVKAARSMFSNMMVKDV 352

Query: 241 -------------------------------WPMLVRF---MSPCAS------------- 300
                                          WP    F   ++ CA+             
Sbjct: 353 ITWNALIAGCTQNGENEEALALFRLLKRESVWPTHYTFGNLLNACANLADLQLGRQAHSH 412

Query: 301 ------------EKEI-------------------------------------------- 307
                       E +I                                            
Sbjct: 413 VLKHGFRFRYGDESDIFVGNSLIDMYMKCGSVENGCRVFEHMLERDCVSWNAMIVGYAQN 472

BLAST of MC10g_new0362 vs. NCBI nr
Match: XP_023552034.1 (pentatricopeptide repeat-containing protein At2g13600 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 390 bits (1002), Expect = 1.23e-127
Identity = 253/575 (44.00%), Postives = 278/575 (48.35%), Query Frame = 0

Query: 1   MPKVDQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLG 60
           MP+VDQ SWNSMISGFEQ DRFDE L YF +MH H F MNEYSFGSALSACA+L+ LK+G
Sbjct: 113 MPQVDQCSWNSMISGFEQHDRFDEALKYFVQMHGHGFFMNEYSFGSALSACAALQDLKMG 172

Query: 61  SQIHSLIYR--------------------------------------------------- 120
           SQIHSLIYR                                                   
Sbjct: 173 SQIHSLIYRSNYLSDMYMGSALVDMYSKCGRVDCARSVFDGMTVRSRVSWNSLITCYEQN 232

Query: 121 -----------------VEADEVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLIL 180
                            VE DEVT ASVVSACAT+S IKEGQQIHARVVKCDEFRNDLIL
Sbjct: 233 GPVDEALSIFVEMIECGVEPDEVTLASVVSACATVSAIKEGQQIHARVVKCDEFRNDLIL 292

Query: 181 GNALVDRYAKYNRINEARLVFNQMSIRSVVSETSMVRGYAKASSVKAARS---------- 240
           GNAL+D YAK NRINEAR+VF++M IRSVVSETSMV GYAKASSVKAARS          
Sbjct: 293 GNALLDMYAKCNRINEARIVFDRMPIRSVVSETSMVSGYAKASSVKAARSMFSNMMVKDV 352

Query: 241 -------------------------------WPMLVRF---MSPCAS------------- 300
                                          WP    F   ++ CA+             
Sbjct: 353 ITWNALIAGCTQNGENEEALTLFRLLKRESVWPTHYTFGNLLNACANLADLQLGRQAHSH 412

Query: 301 ------------EKEI-------------------------------------------- 307
                       E +I                                            
Sbjct: 413 VLKHGFRFRYGDESDIFVGNSLIDMYMKCGSVENGCRVFEHMLERDCVSWNAMIVGYAQN 472

BLAST of MC10g_new0362 vs. NCBI nr
Match: KAG7015869.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 389 bits (998), Expect = 4.64e-127
Identity = 252/575 (43.83%), Postives = 277/575 (48.17%), Query Frame = 0

Query: 1   MPKVDQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLG 60
           MP+VDQ SWNSMISGFEQ DRFDE L YF +MH H F MNEYSFGSALSACA L+ LK+G
Sbjct: 111 MPQVDQCSWNSMISGFEQHDRFDEALKYFVQMHGHGFFMNEYSFGSALSACAGLQDLKMG 170

Query: 61  SQIHSLIYR--------------------------------------------------- 120
           SQIHSLIYR                                                   
Sbjct: 171 SQIHSLIYRSNYLSDMYMGSALVDMYSKCGRVDCARSVFDGMTVRSRVSWNSLITCYEQN 230

Query: 121 -----------------VEADEVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLIL 180
                            VE DEVT ASVVSACAT+S IKEGQQIHARVVKCDEFRNDLIL
Sbjct: 231 GPVDEALSIFVEMIECGVEPDEVTLASVVSACATVSAIKEGQQIHARVVKCDEFRNDLIL 290

Query: 181 GNALVDRYAKYNRINEARLVFNQMSIRSVVSETSMVRGYAKASSVKAARS---------- 240
           GNAL+D YAK NRINEAR+VF++M IRSVVSETSMV GYAKASSVKAARS          
Sbjct: 291 GNALLDMYAKCNRINEARIVFDRMPIRSVVSETSMVSGYAKASSVKAARSMFSNMMVKDV 350

Query: 241 -------------------------------WPMLVRF---MSPCAS------------- 300
                                          WP    F   ++ CA+             
Sbjct: 351 ITWNALIAGCTQNGENEEALTLFRLLKRESVWPTHYTFGNLLNACANLADLQLGRQAHSH 410

Query: 301 ------------EKEI-------------------------------------------- 307
                       E +I                                            
Sbjct: 411 VLKHGFRFRYGDESDIFVGNSLIDMYMKCGSVENGCRVFEHMLERDCVSWNAMIVGYAQN 470

BLAST of MC10g_new0362 vs. ExPASy TrEMBL
Match: A0A6J1BZU9 (pentatricopeptide repeat-containing protein At2g13600-like OS=Momordica charantia OX=3673 GN=LOC111006995 PE=4 SV=1)

HSP 1 Score: 416 bits (1068), Expect = 2.76e-139
Identity = 257/457 (56.24%), Postives = 272/457 (59.52%), Query Frame = 0

Query: 1   MPKVDQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLG 60
           MP+VDQ SWNSMISGFEQ D FDE LNYFA+MHSH F MNEYSFGSALSACA LR LKLG
Sbjct: 111 MPEVDQCSWNSMISGFEQHDCFDEALNYFAQMHSHGFQMNEYSFGSALSACAGLRDLKLG 170

Query: 61  SQIHSLIYR--------------------------------------------------- 120
           SQIHSLIYR                                                   
Sbjct: 171 SQIHSLIYRSNYISDVYMGSALVDMYSKCGRVDRARSVFDGMTVRSRVSWNSLITCYEQN 230

Query: 121 -----------------VEADEVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLIL 180
                            VEADEVT ASVVSACAT+S IK GQQIH RVVKCDEFRNDLIL
Sbjct: 231 GPVDEALVIFFEMIKCRVEADEVTLASVVSACATISAIK-GQQIHTRVVKCDEFRNDLIL 290

Query: 181 GNALVDRYAKYNRINEARLVFNQMSIRSVVSETSM--------------------VRGYA 240
            NALVD YAK NRINEAR+VF+ M IRSVVSETSM                    + GYA
Sbjct: 291 CNALVDMYAKCNRINEARIVFDHMPIRSVVSETSMGVHKMERMKRHLNCVSWNAMIVGYA 350

Query: 241 KAS-------------------------SVKAARSWPMLV-------RFMSP-------- 300
           +                            V  A S   L+       R MS         
Sbjct: 351 QNGFGNEALGVFSKMLESGEKPDHVTMIGVLCACSHAGLLDEGRHYFRSMSAQYGLVSLK 410

Query: 301 --------------CASE-KEI-------PDAIIWGSLLAACKVHRNIKLGEYVVQKLLE 307
                         C  E K+I       PDAIIWGSLLAACKVHRNIKLGEYVV+KLLE
Sbjct: 411 DHYTCMVDLLGRAGCLEEAKDIIEEMPMQPDAIIWGSLLAACKVHRNIKLGEYVVEKLLE 470

BLAST of MC10g_new0362 vs. ExPASy TrEMBL
Match: A0A6J1E1T7 (pentatricopeptide repeat-containing protein At2g13600-like OS=Momordica charantia OX=3673 GN=LOC111025648 PE=4 SV=1)

HSP 1 Score: 405 bits (1042), Expect = 7.72e-134
Identity = 262/577 (45.41%), Postives = 280/577 (48.53%), Query Frame = 0

Query: 1   MPKVDQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLG 60
           MP+VDQ SWNSMISGFEQ DRFDE LNYFA+MHSH FLMNEYSFGSALSACA LR  KLG
Sbjct: 111 MPEVDQCSWNSMISGFEQHDRFDEALNYFAQMHSHGFLMNEYSFGSALSACAGLRDFKLG 170

Query: 61  SQIHSLIYR--------------------------------------------------- 120
           SQIHSLIYR                                                   
Sbjct: 171 SQIHSLIYRSNYLSDVYMGSALVDMYSKCGRVDCARSVFDGMTVRSRVSWNSLITCYEQN 230

Query: 121 -----------------VEADEVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLIL 180
                            VEADEVT ASVVSACAT+S I EGQQIHARVVKCDEFRNDLIL
Sbjct: 231 GPVDEALVIFFEMIKCRVEADEVTLASVVSACATISAINEGQQIHARVVKCDEFRNDLIL 290

Query: 181 GNALVDRYAKYNRINEARLVFNQMSIRSVVSETSMVRGYAKASSVKAARS---------- 240
           GNALVD YAK NRIN+AR+VF++M IRSVVSETSMV GYAKASSVKAAR           
Sbjct: 291 GNALVDMYAKCNRINKARIVFDRMPIRSVVSETSMVSGYAKASSVKAARCMFSNMMVKDV 350

Query: 241 -------------------------------WPMLVRF---MSPCAS------------- 300
                                          WP    F   ++ CA+             
Sbjct: 351 ITWNALIAGCTQNGENEEALILFRLLKRESVWPTHYTFGNLLNACANLADLQLGRQAHSH 410

Query: 301 ------------EKEI-------------------------------------------- 309
                       E +I                                            
Sbjct: 411 VLKHGFRFQSGEESDIFVGNSLIDMYMKCGSVENGCKVFEHMVQRDCVSWNAMIVGYAQN 470

BLAST of MC10g_new0362 vs. ExPASy TrEMBL
Match: A0A6J1EB52 (pentatricopeptide repeat-containing protein At2g13600 OS=Cucurbita moschata OX=3662 GN=LOC111430973 PE=4 SV=1)

HSP 1 Score: 390 bits (1002), Expect = 5.96e-128
Identity = 253/575 (44.00%), Postives = 277/575 (48.17%), Query Frame = 0

Query: 1   MPKVDQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLG 60
           MP+VDQ SWNSMISGFEQ DRFDE L YF +MH H F MNEYSFGSALSACA L+ LK+G
Sbjct: 113 MPQVDQCSWNSMISGFEQHDRFDEALKYFVQMHGHGFFMNEYSFGSALSACAGLQDLKMG 172

Query: 61  SQIHSLIYR--------------------------------------------------- 120
           SQIHSLIYR                                                   
Sbjct: 173 SQIHSLIYRSNYLSDMYMGSALVDMYSKCGRVDCARSVFDGMTVRSRVSWNSLITCYEQN 232

Query: 121 -----------------VEADEVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLIL 180
                            VE DEVT ASVVSACAT+S IKEGQQIHARVVKCDEFRNDLIL
Sbjct: 233 GPVDEALSIFVEMIECGVEPDEVTLASVVSACATVSAIKEGQQIHARVVKCDEFRNDLIL 292

Query: 181 GNALVDRYAKYNRINEARLVFNQMSIRSVVSETSMVRGYAKASSVKAARS---------- 240
           GNAL+D YAK NRINEAR+VF++M IRSVVSETSMV GYAKASSVKAARS          
Sbjct: 293 GNALLDMYAKCNRINEARIVFDRMPIRSVVSETSMVSGYAKASSVKAARSMFSNMMVKDV 352

Query: 241 -------------------------------WPMLVRF---MSPCAS------------- 300
                                          WP    F   ++ CA+             
Sbjct: 353 ITWNALIAGCTQNGENEEALALFRLLKRESVWPTHYTFGNLLNACANLADLQLGRQAHSH 412

Query: 301 ------------EKEI-------------------------------------------- 307
                       E +I                                            
Sbjct: 413 VLKHGFRFRYGDESDIFVGNSLIDMYMKCGSVENGCRVFEHMLERDCVSWNAMIVGYAQN 472

BLAST of MC10g_new0362 vs. ExPASy TrEMBL
Match: A0A6J1HNH1 (pentatricopeptide repeat-containing protein At2g13600 OS=Cucurbita maxima OX=3661 GN=LOC111465245 PE=4 SV=1)

HSP 1 Score: 386 bits (991), Expect = 2.67e-126
Identity = 251/575 (43.65%), Postives = 276/575 (48.00%), Query Frame = 0

Query: 1   MPKVDQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLG 60
           MP+VDQ SWNSMISGFEQ D FDE L YF +MH H F MNEYSFGSALSACA L+ LK+G
Sbjct: 113 MPQVDQCSWNSMISGFEQHDCFDEALKYFVQMHGHGFFMNEYSFGSALSACAGLQDLKMG 172

Query: 61  SQIHSLIYR--------------------------------------------------- 120
           SQIHSLIYR                                                   
Sbjct: 173 SQIHSLIYRSNYLSDMYMGSALVDMYSKCGRVDCARSVFDGMTVRSRVSWNSLITCYEQN 232

Query: 121 -----------------VEADEVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLIL 180
                            VE DEVT ASVVSACAT+S IKEGQQIHARVVKCDEFRNDLIL
Sbjct: 233 GPVDEALSIFVEMIECGVEPDEVTLASVVSACATVSAIKEGQQIHARVVKCDEFRNDLIL 292

Query: 181 GNALVDRYAKYNRINEARLVFNQMSIRSVVSETSMVRGYAKASSVKAARS---------- 240
           GNAL+D YAK NRINEAR+VF++M IRSVVSETSMV GYAKASSVKAARS          
Sbjct: 293 GNALLDMYAKCNRINEARIVFDRMPIRSVVSETSMVSGYAKASSVKAARSMFSNMMVKDV 352

Query: 241 -------------------------------WPMLVRF---MSPCAS------------- 300
                                          WP    F   ++ CA+             
Sbjct: 353 ITWNALIAGCTQNGENEEALTLFRLLKRESVWPTHYTFGNLLNACANLADLQLGRQAHSH 412

Query: 301 ------------EKEI-------------------------------------------- 307
                       E +I                                            
Sbjct: 413 VLKHGFRFRYGDESDIFVGNSLIDMYMKCGSVESGCRVFERMLERDCVSWNAMIVGYAQN 472

BLAST of MC10g_new0362 vs. ExPASy TrEMBL
Match: A0A0A0KJ63 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G003610 PE=4 SV=1)

HSP 1 Score: 370 bits (950), Expect = 3.77e-120
Identity = 242/577 (41.94%), Postives = 271/577 (46.97%), Query Frame = 0

Query: 1   MPKVDQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLG 60
           MP+VDQ SWNSMISGFEQ  RFDE L YFA+MH H FL+NEYSFGSALSACA L+ LKLG
Sbjct: 111 MPQVDQCSWNSMISGFEQHGRFDEALVYFAQMHGHGFLVNEYSFGSALSACAGLQDLKLG 170

Query: 61  SQIHSLIYR--------------------------------------------------- 120
           SQIHSL+YR                                                   
Sbjct: 171 SQIHSLVYRSNYLSDVYMGSALVDMYSKCGRVEYAQSVFDEMTVRSRVSWNSLITCYEQN 230

Query: 121 -----------------VEADEVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLIL 180
                            VE DEVT ASVVSACAT+S IKEGQQIHARVVKCDEFRNDLIL
Sbjct: 231 GPVDEALKIFVEMIKCGVEPDEVTLASVVSACATISAIKEGQQIHARVVKCDEFRNDLIL 290

Query: 181 GNALVDRYAKYNRINEARLVFNQMSIRSVVSETSMVRGYAKASSVKAARS---------- 240
           GNAL+D YAK NRINEAR++F+ M IRSVVSETSMV GYAKAS VK AR           
Sbjct: 291 GNALLDMYAKCNRINEARIIFDMMPIRSVVSETSMVSGYAKASKVKVARYMFSNMMVKDV 350

Query: 241 -------------------------------WPMLVRF---MSPCAS------------- 300
                                          WP    F   ++ CA+             
Sbjct: 351 ITWNALIAGCTQNGENEEALILFRLLKRESVWPTHYTFGNLLNACANLADLQLGRQAHSH 410

Query: 301 -------------------------------------------EKEI------------- 309
                                                      EK+              
Sbjct: 411 VLKHGFRFQYGEDSDVFVGNSLIDMYMKCGSVENGCRVFQHMLEKDCVSWNAMIVGYAQN 470

BLAST of MC10g_new0362 vs. TAIR 10
Match: AT2G13600.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 247.3 bits (630), Expect = 1.7e-65
Identity = 184/584 (31.51%), Postives = 229/584 (39.21%), Query Frame = 0

Query: 1   MPKVDQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLG 60
           MP+ DQ +WNSM+SGF Q DR +E L YFA MH   F++NEYSF S LSAC+ L  +  G
Sbjct: 112 MPERDQCTWNSMVSGFAQHDRCEEALCYFAMMHKEGFVLNEYSFASVLSACSGLNDMNKG 171

Query: 61  SQIHSLI----------------------------------------------------- 120
            Q+HSLI                                                     
Sbjct: 172 VQVHSLIAKSPFLSDVYIGSALVDMYSKCGNVNDAQRVFDEMGDRNVVSWNSLITCFEQN 231

Query: 121 ---------------YRVEADEVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLIL 180
                           RVE DEVT ASV+SACA++S IK GQ++H RVVK D+ RND+IL
Sbjct: 232 GPAVEALDVFQMMLESRVEPDEVTLASVISACASLSAIKVGQEVHGRVVKNDKLRNDIIL 291

Query: 181 GNALVDRYAKYNRINEARLVFNQMSIRSVVSETSMVRGYAKASSVKAAR----------- 240
            NA VD YAK +RI EAR +F+ M IR+V++ETSM+ GYA A+S KAAR           
Sbjct: 292 SNAFVDMYAKCSRIKEARFIFDSMPIRNVIAETSMISGYAMAASTKAARLMFTKMAERNV 351

Query: 241 -SWPMLV--------------------------------RFMSPCASEKEI--------- 300
            SW  L+                                  +  CA   E+         
Sbjct: 352 VSWNALIAGYTQNGENEEALSLFCLLKRESVCPTHYSFANILKACADLAELHLGMQAHVH 411

Query: 301 ------------------------------------------------------------ 317
                                                                       
Sbjct: 412 VLKHGFKFQSGEEDDIFVGNSLIDMYVKCGCVEEGYLVFRKMMERDCVSWNAMIIGFAQN 471

BLAST of MC10g_new0362 vs. TAIR 10
Match: AT2G03880.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 157.1 bits (396), Expect = 2.3e-38
Identity = 107/356 (30.06%), Postives = 155/356 (43.54%), Query Frame = 0

Query: 5   DQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLGSQIH 64
           D   WNS+I GF Q  R D  L  F RM    F+                          
Sbjct: 224 DAIVWNSIIGGFAQNSRSDVALELFKRMKRAGFI-------------------------- 283

Query: 65  SLIYRVEADEVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLILGNALVDRYAKYN 124
                  A++ T  SV+ AC  ++ ++ G Q H  +VK D+   DLIL NALVD Y K  
Sbjct: 284 -------AEQATLTSVLRACTGLALLELGMQAHVHIVKYDQ---DLILNNALVDMYCKCG 343

Query: 125 RINEARLVFNQMSIRSVVSETSMVRGYA---------------KASSVKAARSWPMLVRF 184
            + +A  VFNQM  R V++ ++M+ G A               K+S  K   ++  +V  
Sbjct: 344 SLEDALRVFNQMKERDVITWSTMISGLAQNGYSQEALKLFERMKSSGTKP--NYITIVGV 403

Query: 185 MSPCA-------------SEKEI------------------------------------P 244
           +  C+             S K++                                    P
Sbjct: 404 LFACSHAGLLEDGWYYFRSMKKLYGIDPVREHYGCMIDLLGKAGKLDDAVKLLNEMECEP 463

Query: 245 DAIIWGSLLAACKVHRNIKLGEYVVQKLLEVDAENSGRYVLLSNMYAERGDWGNVVRIRK 297
           DA+ W +LL AC+V RN+ L EY  +K++ +D E++G Y LLSN+YA    W +V  IR 
Sbjct: 464 DAVTWRTLLGACRVQRNMVLAEYAAKKVIALDPEDAGTYTLLSNIYANSQKWDSVEEIRT 523

BLAST of MC10g_new0362 vs. TAIR 10
Match: AT4G02750.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 156.8 bits (395), Expect = 3.0e-38
Identity = 113/386 (29.27%), Postives = 170/386 (44.04%), Query Frame = 0

Query: 1   MPKVDQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLG 60
           MPK D  SW +MI+G+ Q     E L  F +M      +N  SF SALS CA +  L+L 
Sbjct: 369 MPKRDPVSWAAMIAGYSQSGHSFEALRLFVQMEREGGRLNRSSFSSALSTCADVVALEL- 428

Query: 61  SQIHSLIYRVEADEVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLILGNALVDRY 120
                                           G+Q+H R+VK   +     +GNAL+  Y
Sbjct: 429 --------------------------------GKQLHGRLVK-GGYETGCFVGNALLLMY 488

Query: 121 AKYNRINEARLVFNQMSIRSVVSETSMVRGYAKASSVKAARSW-------------PMLV 180
            K   I EA  +F +M+ + +VS  +M+ GY++    + A  +               +V
Sbjct: 489 CKCGSIEEANDLFKEMAGKDIVSWNTMIAGYSRHGFGEVALRFFESMKREGLKPDDATMV 548

Query: 181 RFMSPCASEKEI------------------------------------------------ 240
             +S C+    +                                                
Sbjct: 549 AVLSACSHTGLVDKGRQYFYTMTQDYGVMPNSQHYACMVDLLGRAGLLEDAHNLMKNMPF 608

Query: 241 -PDAIIWGSLLAACKVHRNIKLGEYVVQKLLEVDAENSGRYVLLSNMYAERGDWGNVVRI 300
            PDA IWG+LL A +VH N +L E    K+  ++ ENSG YVLLSN+YA  G WG+V ++
Sbjct: 609 EPDAAIWGTLLGASRVHGNTELAETAADKIFAMEPENSGMYVLLSNLYASSGRWGDVGKL 668

Query: 301 RKLMRKRGVVKHPSCSWIEIQGQLDIFMVKDKRHPKKKEIYMLLRTFLQQMRRAGYV--P 323
           R  MR +GV K P  SWIEIQ +   F V D+ HP+K EI+  L     +M++AGYV   
Sbjct: 669 RVRMRDKGVKKVPGYSWIEIQNKTHTFSVGDEFHPEKDEIFAFLEELDLRMKKAGYVSKT 720

BLAST of MC10g_new0362 vs. TAIR 10
Match: AT3G23330.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 154.5 bits (389), Expect = 1.5e-37
Identity = 100/382 (26.18%), Postives = 173/382 (45.29%), Query Frame = 0

Query: 5   DQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLGSQIH 64
           D  SWNS+++G+ Q  R++E L  F +M                                
Sbjct: 307 DGISWNSLVAGYVQNGRYNEALRLFRQM-------------------------------- 366

Query: 65  SLIYRVEADEVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLILGNALVDRYAKYN 124
            +  +V+   V F+SV+ ACA ++T+  G+Q+H  V++   F +++ + +ALVD Y+K  
Sbjct: 367 -VTAKVKPGAVAFSSVIPACAHLATLHLGKQLHGYVLR-GGFGSNIFIASALVDMYSKCG 426

Query: 125 RINEARLVFNQMSIRSVVSETSMVRGYA---------------KASSVK----------- 184
            I  AR +F++M++   VS T+++ G+A               K   VK           
Sbjct: 427 NIKAARKIFDRMNVLDEVSWTAIIMGHALHGHGHEAVSLFEEMKRQGVKPNQVAFVAVLT 486

Query: 185 -------AARSWPMLVRFMSPCASEKEI-----------------------------PDA 244
                     +W             +E+                             P  
Sbjct: 487 ACSHVGLVDEAWGYFNSMTKVYGLNQELEHYAAVADLLGRAGKLEEAYNFISKMCVEPTG 546

Query: 245 IIWGSLLAACKVHRNIKLGEYVVQKLLEVDAENSGRYVLLSNMYAERGDWGNVVRIRKLM 304
            +W +LL++C VH+N++L E V +K+  VD+EN G YVL+ NMYA  G W  + ++R  M
Sbjct: 547 SVWSTLLSSCSVHKNLELAEKVAEKIFTVDSENMGAYVLMCNMYASNGRWKEMAKLRLRM 606

Query: 305 RKRGVVKHPSCSWIEIQGQLDIFMVKDKRHPKKKEIYMLLRTFLQQMRRAGYVPYVCS-- 323
           RK+G+ K P+CSWIE++ +   F+  D+ HP   +I   L+  ++QM + GYV       
Sbjct: 607 RKKGLRKKPACSWIEMKNKTHGFVSGDRSHPSMDKINEFLKAVMEQMEKEGYVADTSGVL 654

BLAST of MC10g_new0362 vs. TAIR 10
Match: AT3G24000.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 148.3 bits (373), Expect = 1.1e-35
Identity = 107/425 (25.18%), Postives = 179/425 (42.12%), Query Frame = 0

Query: 1   MPKVDQYSWNSMISGFEQRDRFDETLNYFARMHSHCFLMNEYSFGSALSACASLRYLKLG 60
           MP+ D  +W ++ISG+ Q DR  + L +F +M    +  NE++  S + A A+ R    G
Sbjct: 121 MPQRDFVTWTTLISGYSQHDRPCDALLFFNQMLRFGYSPNEFTLSSVIKAAAAERRGCCG 180

Query: 61  SQIHSLIYRVEAD----------------------------------------------- 120
            Q+H    +   D                                               
Sbjct: 181 HQLHGFCVKCGFDSNVHVGSALLDLYTRYGLMDDAQLVFDALESRNDVSWNALIAGHARR 240

Query: 121 ---------------------EVTFASVVSACATMSTIKEGQQIHARVVKCDEFRNDLIL 180
                                  ++AS+  AC++   +++G+ +HA ++K  E +     
Sbjct: 241 SGTEKALELFQGMLRDGFRPSHFSYASLFGACSSTGFLEQGKWVHAYMIKSGE-KLVAFA 300

Query: 181 GNALVDRYAKYNRINEARLVFNQMSIRSVVSETSMVRGYAKASSVKAARSW--------- 240
           GN L+D YAK   I++AR +F++++ R VVS  S++  YA+    K A  W         
Sbjct: 301 GNTLLDMYAKSGSIHDARKIFDRLAKRDVVSWNSLLTAYAQHGFGKEAVWWFEEMRRVGI 360

Query: 241 -PMLVRFMS---PCA--------------------------------------------- 297
            P  + F+S    C+                                             
Sbjct: 361 RPNEISFLSVLTACSHSGLLDEGWHYYELMKKDGIVPEAWHYVTVVDLLGRAGDLNRALR 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SIT72.4e-6431.51Pentatricopeptide repeat-containing protein At2g13600 OS=Arabidopsis thaliana OX... [more]
Q9SI533.3e-3730.06Pentatricopeptide repeat-containing protein At2g03880, mitochondrial OS=Arabidop... [more]
Q9SY024.3e-3729.27Pentatricopeptide repeat-containing protein At4g02750 OS=Arabidopsis thaliana OX... [more]
Q9LW632.1e-3626.18Putative pentatricopeptide repeat-containing protein At3g23330 OS=Arabidopsis th... [more]
Q9LIQ71.5e-3425.18Pentatricopeptide repeat-containing protein At3g24000, mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
XP_022134814.15.70e-13956.24pentatricopeptide repeat-containing protein At2g13600-like [Momordica charantia][more]
XP_022159229.11.60e-13345.41pentatricopeptide repeat-containing protein At2g13600-like [Momordica charantia][more]
XP_022923215.11.23e-12744.00pentatricopeptide repeat-containing protein At2g13600 [Cucurbita moschata][more]
XP_023552034.11.23e-12744.00pentatricopeptide repeat-containing protein At2g13600 [Cucurbita pepo subsp. pep... [more]
KAG7015869.14.64e-12743.83Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub... [more]
Match NameE-valueIdentityDescription
A0A6J1BZU92.76e-13956.24pentatricopeptide repeat-containing protein At2g13600-like OS=Momordica charanti... [more]
A0A6J1E1T77.72e-13445.41pentatricopeptide repeat-containing protein At2g13600-like OS=Momordica charanti... [more]
A0A6J1EB525.96e-12844.00pentatricopeptide repeat-containing protein At2g13600 OS=Cucurbita moschata OX=3... [more]
A0A6J1HNH12.67e-12643.65pentatricopeptide repeat-containing protein At2g13600 OS=Cucurbita maxima OX=366... [more]
A0A0A0KJ633.77e-12041.94Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G003610 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G13600.11.7e-6531.51Pentatricopeptide repeat (PPR) superfamily protein [more]
AT2G03880.12.3e-3830.06Pentatricopeptide repeat (PPR) superfamily protein [more]
AT4G02750.13.0e-3829.27Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G23330.11.5e-3726.18Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G24000.11.1e-3525.18Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 65..301
e-value: 5.5E-19
score: 70.7
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 114..140
e-value: 0.0017
score: 16.4
coord: 7..40
e-value: 6.7E-5
score: 20.8
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 114..137
e-value: 0.26
score: 11.6
coord: 7..35
e-value: 3.6E-4
score: 20.6
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 5..39
score: 9.284279
NoneNo IPR availablePANTHERPTHR47926:SF125PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 35..68
NoneNo IPR availablePANTHERPTHR47926PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 178..321
NoneNo IPR availablePANTHERPTHR47926:SF125PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 71..164
NoneNo IPR availablePANTHERPTHR47926:SF125PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 178..321
NoneNo IPR availablePANTHERPTHR47926PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 35..68
coord: 71..164

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g_new0362.1MC10g_new0362.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding