MC10g_new0239 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g_new0239
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionGDSL esterase/lipase At5g55050-like
LocationMC10: 11036728 .. 11043477 (+)
RNA-Seq ExpressionMC10g_new0239
SyntenyMC10g_new0239
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTAGCAATGGCTTCCCTTCCTTCATCCTTCAGCTTCTTCTTCTTGTTCTTCCTTTCTCATTTGAAAGTTATGGTTGAAAGTTCTCCATTGATTCCCGCCATCTACGTCTTTGGAGACTCTTTGGTTGACGTCGGAAACAACAACTACCTCAATATTTTGGCAAAAGCCGATTTTCCTCACAACGGAGTGGACTTTCCAGACCAACAATACACCGGCAGGTTCTCTAATGGAAAAGTCGTTCCCGATTTTCTCGGTACCATCCATTCTACTCATCTACCAACCACCGTTAAAAAAATTGATAGGTGTTGAAAAATATAATTTCCGCTTATACATTTCTCTCCTAATTGTGTCAAACATATGGCTTAAAACATAACAACTGGTAGGAGATACTATACTACTGACTCTAGTCACTAGCTAGCTACTTTTTTTTGGTCTAATACATAGCATTGTTTAAATAAAATAAAAAAAAGTTTAAACTTTTTTCTAGTAAAAACATACACGTTATGAATCCGATGTAGTAGTTAATTTTTTAAAAAGCTGAGCAAGCATGATTTTAGTTTAGCGGTAATTAGTATATATCTCTGATCAAGACGCCATGAATTCAAATCTCCCACCTCAATTATTGTATTAAAGGAAATTTTTTGAACAGCTGAGGAGGTGGGTTTGCCACTTCTACCACCATATCTCTCCCTTGTAGACGGGAACGGAACCATAACAAATGCGACTTCGTTTATAACCGGCGTCAACTTCGCCTCAGGAGGATCTGGAATTTTGAACGAAACAAGACTAGGTTTTGTAAGTTCCATGCTGATCTCTTCCTATATATATATTTTCTATATATTATTTGAATGGAGAAAGAAAAGAGAGAAAATAACCAATAACTTTTGTTCTTTTGTTTTTTGTTTAAAGAACAAGGTTCAGATTAAAAATAATATCGAGATGGTAGAGATTGAGAGAGACCGACCGATGCCGATGAGCCAACAAATAGATTTATATTCATCAGTGTATGAATATGTAGTAGAAGAGCTGGGATCCGATGCGGCACAAGCACACTTTTCCAAATCCCTGTTGGTGATTGTGGTTGGAAGCAACGACCTGTTCAGTTATTATATGACTGATGCGCACAATGATACCACCCCACAGGAATATGTGGACTTGATGGCCTCCACCTTTGAGTCGGAGCTTAAGGTAATTATTTTAAGAATGACATAACTATAAGCAATAATATTATAGTTTTATAACATTTTCATGTAATTAAATTCTGCTGCATTTTGAAACAACAGCTTTTATACAAGTATGGAGCAAGAAAATTTGTGGCGACTGCAGTTCCATTATTAGGGTGCACTCCATCACAGAGGATGAGCATCCCAGGGGAAGAATGCAAAGATGAAATGAATTACTGGGCTGGAAAGTACAATATTGCCCTTCGGTTATTGTTACAGAGATTGGAAGTGGACCTCCCTGGCTTTTCCTACTCCTATGCTGACATATATGCCATCACCTCTGATATTATGCAAACTCCATCTTCTTATGGTGACATTATTGTGCTATTTTTGTTAAACTTTATGCTTTTCGTTATATTTGAAAATATATATTTTATATTGTATGTTTGGGTAAATGTCTCAAATGTGGTGTGTTCATTTCAGGATTCACAGAAATTAAAGCAGCCTGTTGTGGGCTTGGGCCGTTGAACGCTCTAATTCCCTGCATACCGTTGGCGTCTCTTTGCCCAAATCGAAATGACCACCTCTTTTGGGACCCATTCCATCCCACCCAAAAAGTCAATCACATCGTTGTTGATTACATATTTAATGGATCTAATTTCACGTACCCGATCAATATCAAGGAGCTCATTGATAAACCCACAAAAAGTATTGGGTATCAATATTACTAGCGTTCTAAACAAAGTAGAAGCTAAATAAGTGTACCAATCATGTAATCATCTCTTGTCAATGAAGAAGAACATGTACTTGAGTACTATATTTATGTTAGTGTTTTTAGTTGTTGCAAAAATATTTTTTTTGAATTGTTAAAAACATTCTCGAACTCAAATGTTTACTAGAAAAGTAAAAAAATCCTTAATAGTGCTTTCTAAAAAGTGACTGAGGACGTATGTGTAAGTGTTGGTAGAACGAGTATTTTGAAAAAATTAATTTAAGTAAAATTGATTTTTCAAAATCACCATCATGATCCTTAAAAATCAATTTAAAGTGATTTTAAACATTTTCTAAAACAATTTATGGAATGGGTATAGACTTTAATAGTGAACGAAGACCACTTCTCCATATGAAACCAATGACATAAAAGACAGATACCGAGACAAAGAGCTAGACTTCTGTATTGCGTTGCGAATACGAAGATTTTCTGACGCAGTGTGGGTTGCTGGAAACGGGAGTGATTCCGACAAATACAAAAACCATTTTTTCATACCAAAATTACTCTCAAACACGCTATAAATCTCCTTTGCTAAGATCACTCCTTCGAAAAGCATTTCCAAACCGCTTGATTGGTATAATAAGTATTTTTAGCTTAAAAGTTCCTTATTGATTTCTCACTTGACAGGTAAGCTAATTACTTTTTATATATACTGACTGAAATATTTTCTACAAACCTTTTTTTCACTTTAATATTTTCTACACAGATACTATCTCACCAATTTATTTTTGCCTACACTTTCTCTCGTTTCCTTCGGCTCCACTTGTATTTTTTCTTTCAATTAACCACTCTCTTTATTATATCAACTTTCTTTTACTGATTTATCTCTACCAAATTCATCTCTTACTAATCTCCTTTACACACTTTCTCTTAATTAATTTTTTTCATATACTGTTTTATTTTTAACTTTTTTCATTCTAAACTCCACTGGATAACATTTTCATTTTTATATTCTTTATATTTTTTAAGATTATGTATATTTTTTCATAATATGTTTTTTTACCTTTGTTTTAAGACACATTTAAATTTATAGCCGAATTTTATAAAATAAAACAAAAATAAGTTTTCAAAAACTATATATATTTTTTCTAGTTTTCAAAATATGCTTAGATTTTGAAAAATATTTTTTACAAATGGATAACAAAACGACAATCGAAGTATAGCTCAACAGGTTAAGGCACCTATGTACGGTCGAATGGTCATGAATTTGAATCATCACCACTACATATTGTACTAAAAAAAAGTAGACAACAAAACCTAGGAAGTCATAGGTGTAAGCACGGTTTATAAACTTAAATTTTGAAAATATTTTTACGAACTAGATAACAAACATAAGAAGATAAGGTGTATGTAATGTTTATAAGTTAAATTTTCAAAAATTAAAAACAAAAAACAAATGGTTATCAAAGGGGCCGTAGGATGCATTTGGGCAAGGAGTGAAGTCCACTTGGCTCCTTCAGAAGTAGGAACTTAATTAGATACCGTTGTCCCAAAACTCCAACACAGATTCACTAGCATGCATCACTGCGATGTACAAGACCGACCTCACCCACGAAGTCAGCGTAGAGATGCTCACCCAGCCGAGCATTTAAGTGTTCTTCACATAGAGTAAATTTATGTTGGGTGACCTTCTAGGAAGTTTACGTAATCAGAAAGTGAGTGAAGACAAAGTGTATTATCTGTATTCGTCCGTAGAGACAATGTTTACTCCGAAAACGAACTTAAGTCGCAATCTATATTTCAAATTCGAGATCAAGCCATCATGAAATATAAAAATTCCAAAAAAACTATCAATTCCATGGAGACAAAAATCAAGTTTGATGCACACAAAATTAAGTCAAAGTATATGATCAAAATTTAATTGATCATTAATAAATCTTTTACAACTTATAAATTTCAACGAAAACAGACTTTAATATTGTTTTTCCTACAAAATCTAATTAATTTCATACTAAGCCTATATTTTGCAATTCATTAAACCTATACAACAACGTTACTGTGCAAGCCTCGTATAAAACCACAATTCAATTTGAATATTAAATTAAAATTATCATTTTATAGTAAAAAAGTTATAGTTTCTCGTGTCTTTCTACCTGGTAATGTTACATATTAATACAATATCTTTTAGACATTTGTGCTATCTTTGTTGTGTCATAAAACATGTGCCATTTAGATGGGTCATGATGGGTGTGCCATCCAATTATATGCTTGATTTCTAGTTTCTCATCACGTGCACTCTCTTGTAACATGTGCCCAATTTTCTTTTGAAATTCTATTTATTTGATCCAGTCACCTCTTTAATCTATAATTTGGTTGGATTATTATGGACTCATTGTCCCCATTTGTACGTTTTAAGGATTCTAAGATGAAAACAACAAATTTCGATTTATTATTACTTAATTATTATCCCCAACATAGCATGTGAGGGTTGGGGTTTGGCTACCATCCCTCTATATTAATAAATTAATTAGATTCTTTTTAGAGGAACTATATAAAAATAGAAAAGTACTTCCAACATCTTGCATGCTAGGTTTAAAAAATGAATGTACTCCTTTTTAATAAATATAAAACTTATATTTAAAAATATATTATTCTCTTACTATGCAAGTAAAATTCTTTTTGTGTGAGGTGGAGCATAATTTTTCTTCACCAGTCAGTCGACATTTTTAGTTGTTCTTTTCAAATGTTTTTAGGGATGAAAAGGAAATATATAAACATAGCTTGTAGTTTTAATAGGTATATATTTATTAAGTTTGAGATTAGAAAATTTGAGTTTATATAGCATGTGTTTTAATGGTAAAAAGAACAATAAATTATAGAAACTTGAATTGGTATAACTTTGCAAAAAGTTAGTAAGTCGTCTAACATTTTTATTTGATGTGCGAGCTCAAAATTGTTAAGCCTTGAATTTGGTTGAAAGAATCTATAAGCCTCCTGGAGAAGGTCCTACACATATTGGGCCAAAACATTCACAATAGAGGCACATTCTGCCATAGGCGCTTGAGGTGTTAAATCTACCCAACCCTTCCCTTTACTCTTTGAGCGACGTGGAAGCCATTGTTCAATTATCAACATTCTCAAATTCTAAGAGATGTGCAGAGTGGACGACAGTGCCCCCTTCACATCCTTATAAAGCGGGCCCTCCCCTTTTTGACTGGACTGACTCTCAAGGTCAATCTCCTCCAGGTTTTCTTCGCTCCACGAATGAGAGCTAAACTTAGAGGAAAAAACACGTTCTCTCTTTGCGAGGTCCGAAAGTGGAGAAGCTAAGACCCTTCCAATGCTCCGTTTCACCATGCATAAACTGCATCAAGTAAGCTAGTACTTCTAGTAAAGTTCACTCCTTTGGTATTGAAACAACTCTGATTTTAACAGCAGCAATTTCCTTCTTCTTTACTATTACAGCGGGAGATAGGGCTAAACAAAGCGAAAAGGGGTTTCCATGAGATGGGGGGAGTGTTTAAAGTTCGGGTTATCTCTCTGAATCAGCTTATCCACTGGAGACAATTCGATTGGCTTCACTCCTTCTGGTAATGGGACAAGTTACCCACTGGATCCAAGAGGTAAGGGCTTTGTCTGCCCTTTCTCATAAAGAATTCTGGCTTGGCCTGGGAAAGCAATCGAAGCGGGACGAAGGGCAATGACACGAAATGCATGAAGAGGTTTCATTCAAGATACCAGGAAACCAAATGAAGGAAGACCCCGCAAAATCCTAGTTTTAGGTCTCGTCACATCAATTAGCTCGTGGAGAATATCCAACAACCGATTCCAATGAGTGAATAATGGACCTAGATCCTAAAAACAACAACGCTCCTCATCCTATCCCCATGAGTCTAACAAAGTGCGGACACGAGTCCTAGTCGTTGCGCAGCGGTGCGGCTCACGTCCTCTGGCTCATGCACCGGAATCTTTCACCCACCATTTTTTATTATATAGGAATGAAGATGCTCTTGACTCGACATTATTTGTTCTGTTACATTCGAGCTCTCATTTAAGTCAATAACTAGAAGGAAAAAAAATGGGAATACCAACACATCTGGGAATAAACGATTGGTTTTTGCTAGAGAGATTTTTTTAGAAAGATTCTCACTTAATGGCTGCCGCTTAATGCTCTGGCGCTCTGAATCTCGAAGGTCTTGGTAGGATTCTTACTTGCTGGCCCAGCCCTCGTCGTATCTAGTTTGGTATTGCTACCGCACATGACTTCAGAAGTCATGATGATATTACTAAAGAACGTCTTTATCAAAATATTTTTGCTGCCTAAGGGCATATCAGTTGAAGTAGATTCTTCTTTTTGATCAATCAAAACTCCTTCCTAAAGTGTACAAGCTTCTTCCAAAGCATACGGTGCTGAATATGCAACAGCAATCCAACGACGCATACCCGGACGGAAACTAAGTTCCCACTCTCGACCCATGTAACATGCTACACCAAGTAAGAAGCGTAGAATGACATCCTGGGTTGTTTAGTGATGTCTCCGGCAGCCTTCCTGGGATCTCCAGATCAAATAATTCGCGGTCACGCTTATCCCGGACTGCGTTCTGGAAAAAATTGGCCTACTTGAGAGAAAGGGCGAGCACTCTCGTGGGAAGTGAAGTTAGCCTTCGTGATTCAGATGTTGCTGCTGCTGACATGCAAACCATTGAAGATGGTCCTCGGGGCGAAGAGCTTGAACCAGATAACAATCCCCAAGCTTCTGTTCTTCTTGTTGGTTTTTCTTCCTCACTCTCATTCAAAATGTCAGTCAGTGATTGCGCCATAACAGAGTGCATTAGGGCAGTAGAGATGCCCACGCTTCCTCCTCTGCTTTGA

mRNA sequence

ATGTTAGCAATGGCTTCCCTTCCTTCATCCTTCAGCTTCTTCTTCTTGTTCTTCCTTTCTCATTTGAAAGTTATGGTTGAAAGTTCTCCATTGATTCCCGCCATCTACGTCTTTGGAGACTCTTTGGTTGACGTCGGAAACAACAACTACCTCAATATTTTGGCAAAAGCCGATTTTCCTCACAACGGAGTGGACTTTCCAGACCAACAATACACCGGCAGGTTCTCTAATGGAAAAGTCGTTCCCGATTTTCTCGCTGAGGAGGTGGGTTTGCCACTTCTACCACCATATCTCTCCCTTGTAGACGGGAACGGAACCATAACAAATGCGACTTCGTTTATAACCGGCGTCAACTTCGCCTCAGGAGGATCTGGAATTTTGAACGAAACAAGACTAGGTTTTAACAAGGTTCAGATTAAAAATAATATCGAGATGGTAGAGATTGAGAGAGACCGACCGATGCCGATGAGCCAACAAATAGATTTATATTCATCAGTGTATGAATATGTAGTAGAAGAGCTGGGATCCGATGCGGCACAAGCACACTTTTCCAAATCCCTGTTGGTGATTGTGGTTGGAAGCAACGACCTGTTCAGTTATTATATGACTGATGCGCACAATGATACCACCCCACAGGAATATGTGGACTTGATGGCCTCCACCTTTGAGTCGGAGCTTAAGCTTTTATACAAGTATGGAGCAAGAAAATTTGTGGCGACTGCAGTTCCATTATTAGGGTGCACTCCATCACAGAGGATGAGCATCCCAGGGGAAGAATGCAAAGATGAAATGAATTACTGGGCTGGAAAGTACAATATTGCCCTTCGGTTATTGTTACAGAGATTGGAAGTGGACCTCCCTGGCTTTTCCTACTCCTATGCTGACATATATGCCATCACCTCTGATATTATGCAAACTCCATCTTCTTATGGATTCACAGAAATTAAAGCAGCCTGTTGTGGGCTTGGGCCGTTGAACGCTCTAATTCCCTGCATACCGTTGGCGTCTCTTTGCCCAAATCGAAATGACCACCTCTTTTGGGACCCATTCCATCCCACCCAAAAAGTCAATCACATCGTTGTTGATTACATATTTAATGGATCTAATTTCACGTACCCGATCAATATCAAGGAGCTCATTGATAAACCCACAAAAAGTATTGGTGATGTCTCCGGCAGCCTTCCTGGGATCTCCAGATCAAATAATTCGCGGTCACGCTTATCCCGGACTGCGTTCTGGAAAAAATTGGCCTACTTGAGAGAAAGGGCGAGCACTCTCGTGGGAAGTGAAGTTAGCCTTCGTGATTCAGATGTTGCTGCTGCTGACATGCAAACCATTGAAGATGGTCCTCGGGGCGAAGAGCTTGAACCAGATAACAATCCCCAAGCTTCTGTTCTTCTTGTTGGTTTTTCTTCCTCACTCTCATTCAAAATGTCAGTCAGTGATTGCGCCATAACAGAGTGCATTAGGGCAGTAGAGATGCCCACGCTTCCTCCTCTGCTTTGA

Coding sequence (CDS)

ATGTTAGCAATGGCTTCCCTTCCTTCATCCTTCAGCTTCTTCTTCTTGTTCTTCCTTTCTCATTTGAAAGTTATGGTTGAAAGTTCTCCATTGATTCCCGCCATCTACGTCTTTGGAGACTCTTTGGTTGACGTCGGAAACAACAACTACCTCAATATTTTGGCAAAAGCCGATTTTCCTCACAACGGAGTGGACTTTCCAGACCAACAATACACCGGCAGGTTCTCTAATGGAAAAGTCGTTCCCGATTTTCTCGCTGAGGAGGTGGGTTTGCCACTTCTACCACCATATCTCTCCCTTGTAGACGGGAACGGAACCATAACAAATGCGACTTCGTTTATAACCGGCGTCAACTTCGCCTCAGGAGGATCTGGAATTTTGAACGAAACAAGACTAGGTTTTAACAAGGTTCAGATTAAAAATAATATCGAGATGGTAGAGATTGAGAGAGACCGACCGATGCCGATGAGCCAACAAATAGATTTATATTCATCAGTGTATGAATATGTAGTAGAAGAGCTGGGATCCGATGCGGCACAAGCACACTTTTCCAAATCCCTGTTGGTGATTGTGGTTGGAAGCAACGACCTGTTCAGTTATTATATGACTGATGCGCACAATGATACCACCCCACAGGAATATGTGGACTTGATGGCCTCCACCTTTGAGTCGGAGCTTAAGCTTTTATACAAGTATGGAGCAAGAAAATTTGTGGCGACTGCAGTTCCATTATTAGGGTGCACTCCATCACAGAGGATGAGCATCCCAGGGGAAGAATGCAAAGATGAAATGAATTACTGGGCTGGAAAGTACAATATTGCCCTTCGGTTATTGTTACAGAGATTGGAAGTGGACCTCCCTGGCTTTTCCTACTCCTATGCTGACATATATGCCATCACCTCTGATATTATGCAAACTCCATCTTCTTATGGATTCACAGAAATTAAAGCAGCCTGTTGTGGGCTTGGGCCGTTGAACGCTCTAATTCCCTGCATACCGTTGGCGTCTCTTTGCCCAAATCGAAATGACCACCTCTTTTGGGACCCATTCCATCCCACCCAAAAAGTCAATCACATCGTTGTTGATTACATATTTAATGGATCTAATTTCACGTACCCGATCAATATCAAGGAGCTCATTGATAAACCCACAAAAAGTATTGGTGATGTCTCCGGCAGCCTTCCTGGGATCTCCAGATCAAATAATTCGCGGTCACGCTTATCCCGGACTGCGTTCTGGAAAAAATTGGCCTACTTGAGAGAAAGGGCGAGCACTCTCGTGGGAAGTGAAGTTAGCCTTCGTGATTCAGATGTTGCTGCTGCTGACATGCAAACCATTGAAGATGGTCCTCGGGGCGAAGAGCTTGAACCAGATAACAATCCCCAAGCTTCTGTTCTTCTTGTTGGTTTTTCTTCCTCACTCTCATTCAAAATGTCAGTCAGTGATTGCGCCATAACAGAGTGCATTAGGGCAGTAGAGATGCCCACGCTTCCTCCTCTGCTTTGA

Protein sequence

MLAMASLPSSFSFFFLFFLSHLKVMVESSPLIPAIYVFGDSLVDVGNNNYLNILAKADFPHNGVDFPDQQYTGRFSNGKVVPDFLAEEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGILNETRLGFNKVQIKNNIEMVEIERDRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSLLVIVVGSNDLFSYYMTDAHNDTTPQEYVDLMASTFESELKLLYKYGARKFVATAVPLLGCTPSQRMSIPGEECKDEMNYWAGKYNIALRLLLQRLEVDLPGFSYSYADIYAITSDIMQTPSSYGFTEIKAACCGLGPLNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIFNGSNFTYPINIKELIDKPTKSIGDVSGSLPGISRSNNSRSRLSRTAFWKKLAYLRERASTLVGSEVSLRDSDVAAADMQTIEDGPRGEELEPDNNPQASVLLVGFSSSLSFKMSVSDCAITECIRAVEMPTLPPLL
Homology
BLAST of MC10g_new0239 vs. ExPASy Swiss-Prot
Match: Q9FIA1 (GDSL esterase/lipase At5g55050 OS=Arabidopsis thaliana OX=3702 GN=At5g55050 PE=2 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 3.2e-76
Identity = 153/355 (43.10%), Postives = 223/355 (62.82%), Query Frame = 0

Query: 32  IPAIYVFGDSLVDVGNNNYLNI-LAKADFPHNGVDFPDQQYTGRFSNGKVVPDFLAEEVG 91
           IP +YVFGDSLVD GNNNYL I ++KA++PHNGVDFP+++ TGRF NGK   D +AE+ G
Sbjct: 37  IPGLYVFGDSLVDAGNNNYLPISISKANYPHNGVDFPNKKPTGRFCNGKNAADAIAEKFG 96

Query: 92  LPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGILNETRLGFNKVQIKNNIEMVEIER 151
           LPL PPYLSL          ++ +TGVNFASGG+GI N +                + + 
Sbjct: 97  LPLPPPYLSLRGLLKREKRKSAAVTGVNFASGGAGIFNSS----------------DQKL 156

Query: 152 DRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSLLVIVVGSNDLFSYYMT-DAHNDT 211
            + +P+S+Q++ + S++E V+ +L   AAQ H SKSL  +V+GSNDLF Y+ +      +
Sbjct: 157 GQAIPLSKQVNNWLSIHEEVM-KLEPSAAQLHLSKSLFTVVIGSNDLFDYFGSFKLRRQS 216

Query: 212 TPQEYVDLMASTFESELKLLYKYGARKFVATAVPLLGCTPSQR-MSIPGEECKDEMNYWA 271
            PQ+Y  LMA   + +LK ++  GAR+F+   V  +GCTP +R  +    EC +  N W 
Sbjct: 217 NPQQYTQLMADKLKEQLKRIHDSGARRFLIIGVAQIGCTPGKRAKNSTLHECDEGANMWC 276

Query: 272 GKYNIALRLLLQRLEVDLPG-FSYSYADIYAITSDIMQTPSSYGFTEIKAACCGLGPLNA 331
             YN AL  +LQ+L+ +L G  +Y+Y D Y    DI+  P+ YGF ++ +ACCG G LNA
Sbjct: 277 SLYNEALVKMLQQLKQELQGSITYTYFDNYKSLHDIISNPARYGFADVTSACCGNGELNA 336

Query: 332 LIPCIPLASLCPNRNDHLFWDPF-HPTQKVNHIVVDYIF-NGSNFTYPINIKELI 381
            +PC+PLA LC +R  HLFWD + HPT+     +VD +  + ++++ PI + +L+
Sbjct: 337 DLPCLPLAKLCSDRTKHLFWDRYGHPTEAAARTIVDLMLTDDTHYSSPITLTQLV 374

BLAST of MC10g_new0239 vs. ExPASy Swiss-Prot
Match: O23470 (GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3 SV=2)

HSP 1 Score: 236.9 bits (603), Expect = 5.0e-61
Identity = 136/358 (37.99%), Postives = 199/358 (55.59%), Query Frame = 0

Query: 32  IPAIYVFGDSLVDVGNNNYLNILAKADFPHNGVDFPDQQYTGRFSNGKVVPDFLAEEVGL 91
           IPA +VFGDSLVD GNNNYL  L+KA++  NG+DF     TGRF+NG+ + D + + +G 
Sbjct: 28  IPANFVFGDSLVDAGNNNYLATLSKANYVPNGIDFGSP--TGRFTNGRTIVDIVYQALGS 87

Query: 92  -PLLPPYLSLVDGNGTITNATSFITGVNFASGGSGILNETRLGFNKVQIKNNIEMVEIER 151
             L PPYL+        T+ +  + GVN+ASGGSGILN T   F         E + ++ 
Sbjct: 88  DELTPPYLA------PTTSGSLILNGVNYASGGSGILNSTGKLFG--------ERINVD- 147

Query: 152 DRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSLLVIVVGSNDLFSYYMTDA----- 211
                   Q+D +++  + ++  +G   A   F  ++  +  GSNDL + Y T       
Sbjct: 148 -------AQLDNFATTRQDIISWIGESEAAKLFRSAIFSVTTGSNDLINNYFTPVISTLQ 207

Query: 212 HNDTTPQEYVDLMASTFESELKLLYKYGARKFVATAVPLLGCTPSQRMSIP--GEECKDE 271
                P+ +VD M S F  +L  LY+ GARK V   +  +GC P +R S P  G  C  E
Sbjct: 208 RKVVAPEVFVDTMISKFRLQLTRLYQLGARKIVVINIGPIGCIPFERESDPAAGNNCLAE 267

Query: 272 MNYWAGKYNIALRLLLQRLEVDLPGFSYSYADIYAITSDIMQTPSSYGFTEIKAACCGL- 331
            N  A  YN+ L+ L++ L  +L G  + Y D++ I  DI+Q  SSYGF   K  CC L 
Sbjct: 268 PNEVAQMYNLKLKTLVEELNKNLQGSRFVYGDVFRIVDDIIQNYSSYGFESEKIPCCSLV 327

Query: 332 GPLNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIFNG-SNFTYPINIKEL 380
           G +  LIPC P + +C +R+ ++FWDP+HPT+  N I+   + +G ++  YPINI++L
Sbjct: 328 GKVGGLIPCGPPSKVCMDRSKYVFWDPYHPTEAANIIIARRLLSGDTSDIYPINIRQL 361

BLAST of MC10g_new0239 vs. ExPASy Swiss-Prot
Match: Q9FHQ1 (GDSL esterase/lipase At5g37690 OS=Arabidopsis thaliana OX=3702 GN=At5g37690 PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 2.1e-59
Identity = 123/335 (36.72%), Postives = 190/335 (56.72%), Query Frame = 0

Query: 36  YVFGDSLVDVGNNNYLNI-LAKADFPHNGVDFPDQQYTGRFSNGKVVPDFLAEEVGLPLL 95
           Y+FGDSL +VGNNN+L   LA+ADFP+ GVDF   + TGRF+NG+ + D ++ ++G+   
Sbjct: 29  YIFGDSLTEVGNNNFLQYSLARADFPYYGVDFSGGKATGRFTNGRTIGDIISTKLGILSP 88

Query: 96  PPYLSLVDGNGTITNATSFITGVNFASGGSGILNETRLGFNKVQIKNNIEMVEIERDRPM 155
           PPYLSL        N  +F++G+N+ASGG+GILNET + F             I+R   +
Sbjct: 89  PPYLSLSQ------NDDAFLSGINYASGGAGILNETGIYF-------------IQR---L 148

Query: 156 PMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSLLVIVVGSNDLFSYYMTDAHND---TTP 215
             + QI+ +    E +  ++G  AA  H + ++  I +GSND  + ++     D    T 
Sbjct: 149 TFNDQINCFKKTKEVIRAKIGDGAANKHVNDAMYFIGLGSNDYVNNFLQPFMADGQQYTH 208

Query: 216 QEYVDLMASTFESELKLLYKYGARKFVATAVPLLGCTPSQRMSIPGEECKDEMNYWAGKY 275
            E+V+L+ ST  ++L  +YK GARK +   +  LGC PSQR+      C + +N W  ++
Sbjct: 209 DEFVELLTSTLHNQLTTIYKLGARKVIFHGLGPLGCIPSQRVKSKTRMCLNRVNEWVLEF 268

Query: 276 NIALRLLLQRLEVDLPGFSYSYADIYAITSDIMQTPSSYGFTEIKAACCGLGPLNALIPC 335
           N   + LL  L   LPG  +S+AD Y    D++  P+ YGF     +CC +      + C
Sbjct: 269 NSRTKKLLIDLNKRLPGAKFSFADTYPAVLDLINNPTHYGFKIANTSCCNVDTSVGGL-C 328

Query: 336 IPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIFN 367
           +P + +C NR D +FWD FHP+   N I+ D++F+
Sbjct: 329 LPNSKMCKNRQDFVFWDAFHPSDSANQILADHLFS 340

BLAST of MC10g_new0239 vs. ExPASy Swiss-Prot
Match: Q9FNP2 (GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana OX=3702 GN=At5g08460 PE=2 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 2.6e-57
Identity = 139/380 (36.58%), Postives = 202/380 (53.16%), Query Frame = 0

Query: 13  FFFLFFLSHLKVMVESSPLIPAIYVFGDSLVDVGNNNYLNILAKADFPHNGVDFPDQQYT 72
           F  +F L+  +   E++ + PA++VFGDSLVD GNNN+LN LA++++   G+DF   Q T
Sbjct: 28  FLVVFVLAGGEDSSETTAMFPAMFVFGDSLVDNGNNNHLNSLARSNYLPYGIDFAGNQPT 87

Query: 73  GRFSNGKVVPDFLAEEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGILNET-- 132
           GRFSNGK + DF+ E +GLP +P ++  VDG          + GVN+AS   GIL ET  
Sbjct: 88  GRFSNGKTIVDFIGELLGLPEIPAFMDTVDG------GVDILHGVNYASAAGGILEETGR 147

Query: 133 RLGFNKVQIKNNIEMVEIERDRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSLLVI 192
            LG               ER     M +Q++ +      +   +  ++ + + +KSL+V+
Sbjct: 148 HLG---------------ER---FSMGRQVENFEKTLMEISRSMRKESVKEYMAKSLVVV 207

Query: 193 VVGSNDLFSYYMTD----AHNDTTPQEYVDLMASTFESELKLLYKYGARKFVATAVPLLG 252
            +G+ND  + Y+      + +   P  + DL+ S F + L  LY  G RKFV   V  LG
Sbjct: 208 SLGNNDYINNYLKPRLFLSSSIYDPTSFADLLLSNFTTHLLELYGKGFRKFVIAGVGPLG 267

Query: 253 CTPSQ---RMSIPGEECKDEMNYWAGKYNIALRLLLQRLEVDLPGFS---YSYADIYAIT 312
           C P Q   + ++PG EC + +N  A  +N  L  L+ RL  D    S   + Y + Y   
Sbjct: 268 CIPDQLAAQAALPG-ECVEAVNEMAELFNNRLVSLVDRLNSDNKTASEAIFVYGNTYGAA 327

Query: 313 SDIMQTPSSYGFTEIKAACCGLGPLNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIV 372
            DI+  P +YGF      CCG+G     I C+PLA  C  R+ H+FWD FHPTQ  N I+
Sbjct: 328 VDILTNPFNYGFEVTDRGCCGVGRNRGEITCLPLAVPCAFRDRHVFWDAFHPTQAFNLII 382

Query: 373 VDYIFNGS-NFTYPINIKEL 380
               FNGS +  YPIN+ +L
Sbjct: 388 ALRAFNGSKSDCYPINLSQL 382

BLAST of MC10g_new0239 vs. ExPASy Swiss-Prot
Match: Q8LFJ9 (GDSL esterase/lipase 7 OS=Arabidopsis thaliana OX=3702 GN=GLIP7 PE=2 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.2e-54
Identity = 126/363 (34.71%), Postives = 187/363 (51.52%), Query Frame = 0

Query: 27  ESSPLIPAIYVFGDSLVDVGNNNYLNILAKADFPHNGVDFPDQQYTGRFSNGKVVPDFLA 86
           +  PL PA +VFGDSLVD GNNNY+  LA+A++   G+DF     TGRF NG+ V D+ A
Sbjct: 23  DHQPLAPAFFVFGDSLVDSGNNNYIPTLARANYFPYGIDFGFP--TGRFCNGRTVVDYGA 82

Query: 87  EEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGILNETRLGFNKVQIKNNIEMV 146
             +GLPL+PPYLS       ++   + + GVN+AS  +GIL+ET   +      N     
Sbjct: 83  TYLGLPLVPPYLS------PLSIGQNALRGVNYASAAAGILDETGRHYGARTTFNG---- 142

Query: 147 EIERDRPMPMSQQIDLYSSVYEYVVEELGSDAA--QAHFSKSLLVIVVGSNDLFSYYMTD 206
                       QI  +    E  +     + A  + + +KS++ I +GSND  + Y+  
Sbjct: 143 ------------QISQFEITIELRLRRFFQNPADLRKYLAKSIIGINIGSNDYINNYLMP 202

Query: 207 AHNDTTP----QEYVDLMASTFESELKLLYKYGARKFVATAVPLLGCTPSQRMSIPGEE- 266
               T+     ++Y DL+  T  +++  LY  GARK V      LGC PSQ   + G   
Sbjct: 203 ERYSTSQTYSGEDYADLLIKTLSAQISRLYNLGARKMVLAGSGPLGCIPSQLSMVTGNNT 262

Query: 267 --CKDEMNYWAGKYNIALRLLLQRLEVDLPGFSYSYADIYAITSDIMQTPSSYGFTEIKA 326
             C  ++N     +N  L+ L   L   LPG  + Y +++ +  D++  PS YG      
Sbjct: 263 SGCVTKINNMVSMFNSRLKDLANTLNTTLPGSFFVYQNVFDLFHDMVVNPSRYGLVVSNE 322

Query: 327 ACCGLGPLNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIFNGS-NFTYPINI 380
           ACCG G     + C+PL   C +RN ++FWD FHPT+  N I+    F+ S N++YPI++
Sbjct: 323 ACCGNGRYGGALTCLPLQQPCLDRNQYVFWDAFHPTETANKIIAHNTFSKSANYSYPISV 361

BLAST of MC10g_new0239 vs. NCBI nr
Match: XP_022151306.1 (GDSL esterase/lipase At5g55050-like [Momordica charantia])

HSP 1 Score: 743 bits (1918), Expect = 2.37e-268
Identity = 364/364 (100.00%), Postives = 364/364 (100.00%), Query Frame = 0

Query: 25  MVESSPLIPAIYVFGDSLVDVGNNNYLNILAKADFPHNGVDFPDQQYTGRFSNGKVVPDF 84
           MVESSPLIPAIYVFGDSLVDVGNNNYLNILAKADFPHNGVDFPDQQYTGRFSNGKVVPDF
Sbjct: 1   MVESSPLIPAIYVFGDSLVDVGNNNYLNILAKADFPHNGVDFPDQQYTGRFSNGKVVPDF 60

Query: 85  LAEEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGILNETRLGFNKVQIKNNIE 144
           LAEEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGILNETRLGFNKVQIKNNIE
Sbjct: 61  LAEEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGILNETRLGFNKVQIKNNIE 120

Query: 145 MVEIERDRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSLLVIVVGSNDLFSYYMTD 204
           MVEIERDRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSLLVIVVGSNDLFSYYMTD
Sbjct: 121 MVEIERDRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSLLVIVVGSNDLFSYYMTD 180

Query: 205 AHNDTTPQEYVDLMASTFESELKLLYKYGARKFVATAVPLLGCTPSQRMSIPGEECKDEM 264
           AHNDTTPQEYVDLMASTFESELKLLYKYGARKFVATAVPLLGCTPSQRMSIPGEECKDEM
Sbjct: 181 AHNDTTPQEYVDLMASTFESELKLLYKYGARKFVATAVPLLGCTPSQRMSIPGEECKDEM 240

Query: 265 NYWAGKYNIALRLLLQRLEVDLPGFSYSYADIYAITSDIMQTPSSYGFTEIKAACCGLGP 324
           NYWAGKYNIALRLLLQRLEVDLPGFSYSYADIYAITSDIMQTPSSYGFTEIKAACCGLGP
Sbjct: 241 NYWAGKYNIALRLLLQRLEVDLPGFSYSYADIYAITSDIMQTPSSYGFTEIKAACCGLGP 300

Query: 325 LNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIFNGSNFTYPINIKELIDKPT 384
           LNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIFNGSNFTYPINIKELIDKPT
Sbjct: 301 LNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIFNGSNFTYPINIKELIDKPT 360

Query: 385 KSIG 388
           KSIG
Sbjct: 361 KSIG 364

BLAST of MC10g_new0239 vs. NCBI nr
Match: XP_022156042.1 (GDSL esterase/lipase At5g55050-like [Momordica charantia])

HSP 1 Score: 586 bits (1511), Expect = 1.64e-206
Identity = 279/364 (76.65%), Postives = 312/364 (85.71%), Query Frame = 0

Query: 25  MVESSPLIPAIYVFGDSLVDVGNNNYLNILAKADFPHNGVDFPDQQYTGRFSNGKVVPDF 84
           MVESSPL+PA+Y+FGDSLVD GNNNYLN  AKA+FPHNGVDFP QQYTGRFSNGK+VPDF
Sbjct: 1   MVESSPLVPAVYIFGDSLVDAGNNNYLNASAKANFPHNGVDFPHQQYTGRFSNGKIVPDF 60

Query: 85  LAEEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGILNETRLGFNKVQIKNNIE 144
           LAEEV LPL+PPYLSL D NG +TNAT F TGVNFASGG GIL+ET+ GF KV IKN +E
Sbjct: 61  LAEEVSLPLVPPYLSLADENGRVTNATPFRTGVNFASGGFGILDETKQGFKKVPIKNKVE 120

Query: 145 MVEIERDRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSLLVIVVGSNDLFSYYMTD 204
           M   E+DRPMPMSQQI  YSSVYEY+VEELGSDAAQAH S+SL  IV GSNDLF+YY TD
Sbjct: 121 M---EQDRPMPMSQQIAFYSSVYEYLVEELGSDAAQAHLSQSLFFIVAGSNDLFAYYETD 180

Query: 205 AHNDTTPQEYVDLMASTFESELKLLYKYGARKFVATAVPLLGCTPSQRMSIPGEECKDEM 264
            H   TPQ YVDLM STFESELK+LYKYGARK+V   V LLGC P QRM+I G EC+DEM
Sbjct: 181 VHKHYTPQAYVDLMVSTFESELKVLYKYGARKYVVNGVGLLGCIPFQRMNIQGGECRDEM 240

Query: 265 NYWAGKYNIALRLLLQRLEVDLPGFSYSYADIYAITSDIMQTPSSYGFTEIKAACCGLGP 324
           NYWAGKYN ALR LL RLE++LPGF+YSY D+YA+ S +M+TPS YGF EI+AACCGLG 
Sbjct: 241 NYWAGKYNTALRSLLHRLEMELPGFAYSYGDVYALISHVMETPSKYGFIEIEAACCGLGH 300

Query: 325 LNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIFNGSNFTYPINIKELIDKPT 384
           LNA++PCIP+ASLCPNR+DHLFWDPFHP+QKVN IVVDY+F+G NFTYPINIKELI KP 
Sbjct: 301 LNAVVPCIPMASLCPNRSDHLFWDPFHPSQKVNRIVVDYMFDGFNFTYPINIKELIAKPA 360

Query: 385 KSIG 388
           KSIG
Sbjct: 361 KSIG 361

BLAST of MC10g_new0239 vs. NCBI nr
Match: XP_022151286.1 (GDSL esterase/lipase At5g55050-like [Momordica charantia])

HSP 1 Score: 410 bits (1054), Expect = 3.66e-137
Identity = 211/374 (56.42%), Postives = 264/374 (70.59%), Query Frame = 0

Query: 10  SFSFFFLFFLSHLKVMV-ESSPLIPAIYVFGDSLVDVGNNNYLNI-LAKADFPHNGVDFP 69
           +F FFF+F        V E   L+PAIYVFGDSLVDVGNNN+L + LAKA+FPHNG+DFP
Sbjct: 5   AFFFFFIFLCVSFSFRVCECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNGIDFP 64

Query: 70  DQQYTGRFSNGKVVPDFLAEEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGIL 129
             + TGRFSNGK   D+LAE+VGLP  PPYLSLV     ITN T F TGV+FASGG+GI 
Sbjct: 65  TTKPTGRFSNGKNAADYLAEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGGAGIF 124

Query: 130 NETRLGFNKVQIKNNIEMVEIERDRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSL 189
           NET          NN+    +       M QQID YSSVY+++VEELGS AAQ H SKSL
Sbjct: 125 NET----------NNLFKQSVA------MGQQIDFYSSVYQHLVEELGSSAAQVHLSKSL 184

Query: 190 LVIVVGSNDLFSYY-MTDAHNDTTPQEYVDLMASTFESELKLLYKYGARKFVATAVPLLG 249
             +V+GSND+F Y+  +D     +PQ+YVDLMAST ES+LK LY YGARK+V   V L+G
Sbjct: 185 FTVVIGSNDIFGYHESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVG 244

Query: 250 CTPSQRMSIPGEECKDEMNYWAGKYNIALRLLLQRLEVDLPGFSYSYADIYAITSDIMQT 309
           CTPSQR      EC  E+N W   YN  L+ +L RL+++L G ++SY D YA+ S+ ++T
Sbjct: 245 CTPSQRKRSRTGECDAEVNSWVEHYNFGLKAMLGRLKMELNGIAFSYFDAYAVMSNFIRT 304

Query: 310 PSSYGFTEIKAACCGLGPLNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIFN 369
           PSSYGFTEI+ ACCGLG LNA +PC+P+A+ C NRNDHLFWD +HPTQ+ + +  +YIF+
Sbjct: 305 PSSYGFTEIRRACCGLGNLNADVPCLPIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFD 361

Query: 370 GSNFTYPINIKELI 380
           G  FTY +N+K+LI
Sbjct: 365 GP-FTYALNVKQLI 361

BLAST of MC10g_new0239 vs. NCBI nr
Match: XP_038903279.1 (GDSL esterase/lipase At5g55050 [Benincasa hispida])

HSP 1 Score: 404 bits (1039), Expect = 7.28e-135
Identity = 202/375 (53.87%), Postives = 264/375 (70.40%), Query Frame = 0

Query: 8   PSSFSFFFLFFLSHLKVMVESSPLIPAIYVFGDSLVDVGNNNYLNI-LAKADFPHNGVDF 67
           PSSFS  F    S     V +S L+PAIYVFGDSLVDVGNNN+L + LAKA+FPHNG+DF
Sbjct: 6   PSSFSSLFFLIFSIYLFRVSNSALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNGLDF 65

Query: 68  PDQQYTGRFSNGKVVPDFLAEEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGI 127
           P ++ TGRFSNGK   D++AE+VGLP  PPYLSL+     +TN T F TGV+FASGG+GI
Sbjct: 66  PTKKPTGRFSNGKNAADYVAEKVGLPTSPPYLSLISKFRKVTNTTPFKTGVSFASGGAGI 125

Query: 128 LNETRLGFNKVQIKNNIEMVEIERDRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKS 187
            NET   F +                 + M QQI+ YS VY+ +V ELGS  A  H SKS
Sbjct: 126 FNETNKLFKQ----------------SVAMDQQIEFYSLVYKTLVGELGSPGAAEHLSKS 185

Query: 188 LLVIVVGSNDLFSYY-MTDAHNDTTPQEYVDLMASTFESELKLLYKYGARKFVATAVPLL 247
           L  +V+GSND+F Y+  +D  N  +PQ+YVD MAST +S+LK LY YGARK+V   +  +
Sbjct: 186 LFTVVIGSNDIFGYHESSDLRNKYSPQQYVDSMASTLKSQLKRLYGYGARKYVVGGIGPV 245

Query: 248 GCTPSQRMSIPGEECKDEMNYWAGKYNIALRLLLQRLEVDLPGFSYSYADIYAITSDIMQ 307
           GC PSQR    G EC +E+N WA  YNIAL+ +LQ L+++L   SYSY D+Y + S+ ++
Sbjct: 246 GCAPSQRKRSKGGECDEEVNNWAAIYNIALKSMLQTLKMELKDISYSYFDVYQVMSNFLR 305

Query: 308 TPSSYGFTEIKAACCGLGPLNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIF 367
           +PSS+GFTEIK+ACCGLG L A +PC+P+A+ CPNRN+HLFWD +HPTQ+ +++  +YIF
Sbjct: 306 SPSSFGFTEIKSACCGLGNLKADVPCLPIATFCPNRNNHLFWDLYHPTQQAHYMFANYIF 363

Query: 368 NGSNFTYPINIKELI 380
           +G  FTYP+N+ +LI
Sbjct: 366 DGP-FTYPLNVNQLI 363

BLAST of MC10g_new0239 vs. NCBI nr
Match: XP_022932219.1 (GDSL esterase/lipase At5g55050 [Cucurbita moschata])

HSP 1 Score: 399 bits (1025), Expect = 8.59e-133
Identity = 200/375 (53.33%), Postives = 266/375 (70.93%), Query Frame = 0

Query: 9   SSFSFFFLFFLSHLKVMVESSPLIPAIYVFGDSLVDVGNNNYLNI-LAKADFPHNGVDFP 68
           S   FFF+F L  +   +  S L+PAIYVFGDSLVDVGNNN+L + +AKA+FPHNG+DFP
Sbjct: 3   SQILFFFMFSLLSIFSTISESALVPAIYVFGDSLVDVGNNNHLKLSIAKANFPHNGIDFP 62

Query: 69  DQQYTGRFSNGKVVPDFLAEEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGIL 128
            ++ TGRFSNGK   D++AE+VGLP  PPYLSL+     + N   F TGV+FASGG+GI 
Sbjct: 63  TKKPTGRFSNGKNAADYIAEKVGLPTSPPYLSLISKFRKVINTKPFKTGVSFASGGAGIF 122

Query: 129 NETRLGFNKVQIKNNIEMVEIERDRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSL 188
           NET          NN+    +  D      QQI+ YS VY+ +V ELG  AAQAH SKSL
Sbjct: 123 NET----------NNLFKQSVAMD------QQIEYYSLVYQKLVVELGLSAAQAHLSKSL 182

Query: 189 LVIVVGSNDLFSYY-MTDAHNDTTPQEYVDLMASTFESELKLLYKYGARKFVATAVPLLG 248
             +V+GSND+F Y+  +D     +PQ+YV+ MAST +S+LK LY YGARK+V T + L+G
Sbjct: 183 FTVVIGSNDIFGYHESSDLRKKYSPQQYVESMASTLKSQLKRLYGYGARKYVVTGIGLVG 242

Query: 249 CTPSQRMSIPGEECKDEMNYWAGKYNIALRLLLQRLEVDLPG-FSYSYADIYAITSDIMQ 308
           CTPSQR      EC +E+N WA +YN+AL+ +LQ L+++L    S++Y ++YA+ SD ++
Sbjct: 243 CTPSQRKRSKTGECDEEVNSWAAEYNVALKSMLQTLKMELNNNISFTYFEVYAVMSDFIR 302

Query: 309 TPSSYGFTEIKAACCGLGPLNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIF 368
           +PSS+GFTEIK+ACCG G LNA +PC+P+A+ C NRN HLFWD +HPTQ+ + ++ DYIF
Sbjct: 303 SPSSFGFTEIKSACCGHGHLNADLPCLPIAAFCSNRNSHLFWDLYHPTQEASRVLADYIF 360

Query: 369 NGSNFTYPINIKELI 380
           NG  FTYPIN+ +LI
Sbjct: 363 NGP-FTYPINVNQLI 360

BLAST of MC10g_new0239 vs. ExPASy TrEMBL
Match: A0A6J1DE87 (GDSL esterase/lipase At5g55050-like OS=Momordica charantia OX=3673 GN=LOC111019272 PE=3 SV=1)

HSP 1 Score: 743 bits (1918), Expect = 1.15e-268
Identity = 364/364 (100.00%), Postives = 364/364 (100.00%), Query Frame = 0

Query: 25  MVESSPLIPAIYVFGDSLVDVGNNNYLNILAKADFPHNGVDFPDQQYTGRFSNGKVVPDF 84
           MVESSPLIPAIYVFGDSLVDVGNNNYLNILAKADFPHNGVDFPDQQYTGRFSNGKVVPDF
Sbjct: 1   MVESSPLIPAIYVFGDSLVDVGNNNYLNILAKADFPHNGVDFPDQQYTGRFSNGKVVPDF 60

Query: 85  LAEEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGILNETRLGFNKVQIKNNIE 144
           LAEEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGILNETRLGFNKVQIKNNIE
Sbjct: 61  LAEEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGILNETRLGFNKVQIKNNIE 120

Query: 145 MVEIERDRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSLLVIVVGSNDLFSYYMTD 204
           MVEIERDRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSLLVIVVGSNDLFSYYMTD
Sbjct: 121 MVEIERDRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSLLVIVVGSNDLFSYYMTD 180

Query: 205 AHNDTTPQEYVDLMASTFESELKLLYKYGARKFVATAVPLLGCTPSQRMSIPGEECKDEM 264
           AHNDTTPQEYVDLMASTFESELKLLYKYGARKFVATAVPLLGCTPSQRMSIPGEECKDEM
Sbjct: 181 AHNDTTPQEYVDLMASTFESELKLLYKYGARKFVATAVPLLGCTPSQRMSIPGEECKDEM 240

Query: 265 NYWAGKYNIALRLLLQRLEVDLPGFSYSYADIYAITSDIMQTPSSYGFTEIKAACCGLGP 324
           NYWAGKYNIALRLLLQRLEVDLPGFSYSYADIYAITSDIMQTPSSYGFTEIKAACCGLGP
Sbjct: 241 NYWAGKYNIALRLLLQRLEVDLPGFSYSYADIYAITSDIMQTPSSYGFTEIKAACCGLGP 300

Query: 325 LNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIFNGSNFTYPINIKELIDKPT 384
           LNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIFNGSNFTYPINIKELIDKPT
Sbjct: 301 LNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIFNGSNFTYPINIKELIDKPT 360

Query: 385 KSIG 388
           KSIG
Sbjct: 361 KSIG 364

BLAST of MC10g_new0239 vs. ExPASy TrEMBL
Match: A0A6J1DQZ9 (GDSL esterase/lipase At5g55050-like OS=Momordica charantia OX=3673 GN=LOC111023011 PE=3 SV=1)

HSP 1 Score: 586 bits (1511), Expect = 7.95e-207
Identity = 279/364 (76.65%), Postives = 312/364 (85.71%), Query Frame = 0

Query: 25  MVESSPLIPAIYVFGDSLVDVGNNNYLNILAKADFPHNGVDFPDQQYTGRFSNGKVVPDF 84
           MVESSPL+PA+Y+FGDSLVD GNNNYLN  AKA+FPHNGVDFP QQYTGRFSNGK+VPDF
Sbjct: 1   MVESSPLVPAVYIFGDSLVDAGNNNYLNASAKANFPHNGVDFPHQQYTGRFSNGKIVPDF 60

Query: 85  LAEEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGILNETRLGFNKVQIKNNIE 144
           LAEEV LPL+PPYLSL D NG +TNAT F TGVNFASGG GIL+ET+ GF KV IKN +E
Sbjct: 61  LAEEVSLPLVPPYLSLADENGRVTNATPFRTGVNFASGGFGILDETKQGFKKVPIKNKVE 120

Query: 145 MVEIERDRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSLLVIVVGSNDLFSYYMTD 204
           M   E+DRPMPMSQQI  YSSVYEY+VEELGSDAAQAH S+SL  IV GSNDLF+YY TD
Sbjct: 121 M---EQDRPMPMSQQIAFYSSVYEYLVEELGSDAAQAHLSQSLFFIVAGSNDLFAYYETD 180

Query: 205 AHNDTTPQEYVDLMASTFESELKLLYKYGARKFVATAVPLLGCTPSQRMSIPGEECKDEM 264
            H   TPQ YVDLM STFESELK+LYKYGARK+V   V LLGC P QRM+I G EC+DEM
Sbjct: 181 VHKHYTPQAYVDLMVSTFESELKVLYKYGARKYVVNGVGLLGCIPFQRMNIQGGECRDEM 240

Query: 265 NYWAGKYNIALRLLLQRLEVDLPGFSYSYADIYAITSDIMQTPSSYGFTEIKAACCGLGP 324
           NYWAGKYN ALR LL RLE++LPGF+YSY D+YA+ S +M+TPS YGF EI+AACCGLG 
Sbjct: 241 NYWAGKYNTALRSLLHRLEMELPGFAYSYGDVYALISHVMETPSKYGFIEIEAACCGLGH 300

Query: 325 LNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIFNGSNFTYPINIKELIDKPT 384
           LNA++PCIP+ASLCPNR+DHLFWDPFHP+QKVN IVVDY+F+G NFTYPINIKELI KP 
Sbjct: 301 LNAVVPCIPMASLCPNRSDHLFWDPFHPSQKVNRIVVDYMFDGFNFTYPINIKELIAKPA 360

Query: 385 KSIG 388
           KSIG
Sbjct: 361 KSIG 361

BLAST of MC10g_new0239 vs. ExPASy TrEMBL
Match: A0A6J1DE65 (GDSL esterase/lipase At5g55050-like OS=Momordica charantia OX=3673 GN=LOC111019250 PE=3 SV=1)

HSP 1 Score: 410 bits (1054), Expect = 1.77e-137
Identity = 211/374 (56.42%), Postives = 264/374 (70.59%), Query Frame = 0

Query: 10  SFSFFFLFFLSHLKVMV-ESSPLIPAIYVFGDSLVDVGNNNYLNI-LAKADFPHNGVDFP 69
           +F FFF+F        V E   L+PAIYVFGDSLVDVGNNN+L + LAKA+FPHNG+DFP
Sbjct: 5   AFFFFFIFLCVSFSFRVCECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNGIDFP 64

Query: 70  DQQYTGRFSNGKVVPDFLAEEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGIL 129
             + TGRFSNGK   D+LAE+VGLP  PPYLSLV     ITN T F TGV+FASGG+GI 
Sbjct: 65  TTKPTGRFSNGKNAADYLAEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGGAGIF 124

Query: 130 NETRLGFNKVQIKNNIEMVEIERDRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSL 189
           NET          NN+    +       M QQID YSSVY+++VEELGS AAQ H SKSL
Sbjct: 125 NET----------NNLFKQSVA------MGQQIDFYSSVYQHLVEELGSSAAQVHLSKSL 184

Query: 190 LVIVVGSNDLFSYY-MTDAHNDTTPQEYVDLMASTFESELKLLYKYGARKFVATAVPLLG 249
             +V+GSND+F Y+  +D     +PQ+YVDLMAST ES+LK LY YGARK+V   V L+G
Sbjct: 185 FTVVIGSNDIFGYHESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVG 244

Query: 250 CTPSQRMSIPGEECKDEMNYWAGKYNIALRLLLQRLEVDLPGFSYSYADIYAITSDIMQT 309
           CTPSQR      EC  E+N W   YN  L+ +L RL+++L G ++SY D YA+ S+ ++T
Sbjct: 245 CTPSQRKRSRTGECDAEVNSWVEHYNFGLKAMLGRLKMELNGIAFSYFDAYAVMSNFIRT 304

Query: 310 PSSYGFTEIKAACCGLGPLNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIFN 369
           PSSYGFTEI+ ACCGLG LNA +PC+P+A+ C NRNDHLFWD +HPTQ+ + +  +YIF+
Sbjct: 305 PSSYGFTEIRRACCGLGNLNADVPCLPIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFD 361

Query: 370 GSNFTYPINIKELI 380
           G  FTY +N+K+LI
Sbjct: 365 GP-FTYALNVKQLI 361

BLAST of MC10g_new0239 vs. ExPASy TrEMBL
Match: A0A6J1EWE7 (GDSL esterase/lipase At5g55050 OS=Cucurbita moschata OX=3662 GN=LOC111438588 PE=3 SV=1)

HSP 1 Score: 399 bits (1025), Expect = 4.16e-133
Identity = 200/375 (53.33%), Postives = 266/375 (70.93%), Query Frame = 0

Query: 9   SSFSFFFLFFLSHLKVMVESSPLIPAIYVFGDSLVDVGNNNYLNI-LAKADFPHNGVDFP 68
           S   FFF+F L  +   +  S L+PAIYVFGDSLVDVGNNN+L + +AKA+FPHNG+DFP
Sbjct: 3   SQILFFFMFSLLSIFSTISESALVPAIYVFGDSLVDVGNNNHLKLSIAKANFPHNGIDFP 62

Query: 69  DQQYTGRFSNGKVVPDFLAEEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGIL 128
            ++ TGRFSNGK   D++AE+VGLP  PPYLSL+     + N   F TGV+FASGG+GI 
Sbjct: 63  TKKPTGRFSNGKNAADYIAEKVGLPTSPPYLSLISKFRKVINTKPFKTGVSFASGGAGIF 122

Query: 129 NETRLGFNKVQIKNNIEMVEIERDRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSL 188
           NET          NN+    +  D      QQI+ YS VY+ +V ELG  AAQAH SKSL
Sbjct: 123 NET----------NNLFKQSVAMD------QQIEYYSLVYQKLVVELGLSAAQAHLSKSL 182

Query: 189 LVIVVGSNDLFSYY-MTDAHNDTTPQEYVDLMASTFESELKLLYKYGARKFVATAVPLLG 248
             +V+GSND+F Y+  +D     +PQ+YV+ MAST +S+LK LY YGARK+V T + L+G
Sbjct: 183 FTVVIGSNDIFGYHESSDLRKKYSPQQYVESMASTLKSQLKRLYGYGARKYVVTGIGLVG 242

Query: 249 CTPSQRMSIPGEECKDEMNYWAGKYNIALRLLLQRLEVDLPG-FSYSYADIYAITSDIMQ 308
           CTPSQR      EC +E+N WA +YN+AL+ +LQ L+++L    S++Y ++YA+ SD ++
Sbjct: 243 CTPSQRKRSKTGECDEEVNSWAAEYNVALKSMLQTLKMELNNNISFTYFEVYAVMSDFIR 302

Query: 309 TPSSYGFTEIKAACCGLGPLNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIF 368
           +PSS+GFTEIK+ACCG G LNA +PC+P+A+ C NRN HLFWD +HPTQ+ + ++ DYIF
Sbjct: 303 SPSSFGFTEIKSACCGHGHLNADLPCLPIAAFCSNRNSHLFWDLYHPTQEASRVLADYIF 360

Query: 369 NGSNFTYPINIKELI 380
           NG  FTYPIN+ +LI
Sbjct: 363 NGP-FTYPINVNQLI 360

BLAST of MC10g_new0239 vs. ExPASy TrEMBL
Match: A0A6J1HMQ2 (GDSL esterase/lipase At5g55050 OS=Cucurbita maxima OX=3661 GN=LOC111465054 PE=3 SV=1)

HSP 1 Score: 393 bits (1010), Expect = 7.69e-131
Identity = 200/375 (53.33%), Postives = 264/375 (70.40%), Query Frame = 0

Query: 9   SSFSFFFLFFLSHLKVMVESSPLIPAIYVFGDSLVDVGNNNYLNI-LAKADFPHNGVDFP 68
           S   F F+F L  +   +  S L+PAIYVFGDSLVDVGNNN+L + +AKA+FPHNG+DFP
Sbjct: 3   SQLLFCFIFSLLSIFSTISESALVPAIYVFGDSLVDVGNNNHLKLSIAKANFPHNGIDFP 62

Query: 69  DQQYTGRFSNGKVVPDFLAEEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGIL 128
            ++ TGRFSNGK   D++AE+VGLP  PPYLSL+     + N   F TGV+FASGG+GI 
Sbjct: 63  TKKPTGRFSNGKNAADYIAEKVGLPTSPPYLSLISKFRKVINTKPFKTGVSFASGGAGIF 122

Query: 129 NETRLGFNKVQIKNNIEMVEIERDRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSL 188
           NET          NN+    +  D      QQI+ YS VY+ +V ELG  AA+AH SKSL
Sbjct: 123 NET----------NNLFKQSVAID------QQIEYYSLVYQNLVVELGLSAAEAHLSKSL 182

Query: 189 LVIVVGSNDLFSYY-MTDAHNDTTPQEYVDLMASTFESELKLLYKYGARKFVATAVPLLG 248
             +V+GSND+F Y+  +D     +PQ+YV+ MAST +S+LK LY YGARK+V T + L+G
Sbjct: 183 FTVVIGSNDIFGYHESSDLRKKYSPQQYVESMASTLKSQLKRLYGYGARKYVVTGIGLVG 242

Query: 249 CTPSQRMSIPGEECKDEMNYWAGKYNIALRLLLQRLEVDLPG-FSYSYADIYAITSDIMQ 308
           CTPSQR      EC  E+N WA +YN+AL+ +LQ L+++L    S+SY ++YAI SD ++
Sbjct: 243 CTPSQRKRSKTGECDVEVNSWAAQYNVALKSMLQTLKMELNNNISFSYFEVYAIMSDFVR 302

Query: 309 TPSSYGFTEIKAACCGLGPLNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIF 368
           +PSSYGFTEI++ACCG G LNA +PC+P+A+ C NRN HLFWD +HPTQ+ + ++ DYIF
Sbjct: 303 SPSSYGFTEIRSACCGHGHLNADLPCLPIAAFCSNRNSHLFWDLYHPTQEASRMLADYIF 360

Query: 369 NGSNFTYPINIKELI 380
           NG  FTYPIN+ +LI
Sbjct: 363 NGP-FTYPINVNQLI 360

BLAST of MC10g_new0239 vs. TAIR 10
Match: AT5G55050.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 287.3 bits (734), Expect = 2.3e-77
Identity = 153/355 (43.10%), Postives = 223/355 (62.82%), Query Frame = 0

Query: 32  IPAIYVFGDSLVDVGNNNYLNI-LAKADFPHNGVDFPDQQYTGRFSNGKVVPDFLAEEVG 91
           IP +YVFGDSLVD GNNNYL I ++KA++PHNGVDFP+++ TGRF NGK   D +AE+ G
Sbjct: 37  IPGLYVFGDSLVDAGNNNYLPISISKANYPHNGVDFPNKKPTGRFCNGKNAADAIAEKFG 96

Query: 92  LPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGILNETRLGFNKVQIKNNIEMVEIER 151
           LPL PPYLSL          ++ +TGVNFASGG+GI N +                + + 
Sbjct: 97  LPLPPPYLSLRGLLKREKRKSAAVTGVNFASGGAGIFNSS----------------DQKL 156

Query: 152 DRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSLLVIVVGSNDLFSYYMT-DAHNDT 211
            + +P+S+Q++ + S++E V+ +L   AAQ H SKSL  +V+GSNDLF Y+ +      +
Sbjct: 157 GQAIPLSKQVNNWLSIHEEVM-KLEPSAAQLHLSKSLFTVVIGSNDLFDYFGSFKLRRQS 216

Query: 212 TPQEYVDLMASTFESELKLLYKYGARKFVATAVPLLGCTPSQR-MSIPGEECKDEMNYWA 271
            PQ+Y  LMA   + +LK ++  GAR+F+   V  +GCTP +R  +    EC +  N W 
Sbjct: 217 NPQQYTQLMADKLKEQLKRIHDSGARRFLIIGVAQIGCTPGKRAKNSTLHECDEGANMWC 276

Query: 272 GKYNIALRLLLQRLEVDLPG-FSYSYADIYAITSDIMQTPSSYGFTEIKAACCGLGPLNA 331
             YN AL  +LQ+L+ +L G  +Y+Y D Y    DI+  P+ YGF ++ +ACCG G LNA
Sbjct: 277 SLYNEALVKMLQQLKQELQGSITYTYFDNYKSLHDIISNPARYGFADVTSACCGNGELNA 336

Query: 332 LIPCIPLASLCPNRNDHLFWDPF-HPTQKVNHIVVDYIF-NGSNFTYPINIKELI 381
            +PC+PLA LC +R  HLFWD + HPT+     +VD +  + ++++ PI + +L+
Sbjct: 337 DLPCLPLAKLCSDRTKHLFWDRYGHPTEAAARTIVDLMLTDDTHYSSPITLTQLV 374

BLAST of MC10g_new0239 vs. TAIR 10
Match: AT5G37690.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 231.5 bits (589), Expect = 1.5e-60
Identity = 123/335 (36.72%), Postives = 190/335 (56.72%), Query Frame = 0

Query: 36  YVFGDSLVDVGNNNYLNI-LAKADFPHNGVDFPDQQYTGRFSNGKVVPDFLAEEVGLPLL 95
           Y+FGDSL +VGNNN+L   LA+ADFP+ GVDF   + TGRF+NG+ + D ++ ++G+   
Sbjct: 29  YIFGDSLTEVGNNNFLQYSLARADFPYYGVDFSGGKATGRFTNGRTIGDIISTKLGILSP 88

Query: 96  PPYLSLVDGNGTITNATSFITGVNFASGGSGILNETRLGFNKVQIKNNIEMVEIERDRPM 155
           PPYLSL        N  +F++G+N+ASGG+GILNET + F             I+R   +
Sbjct: 89  PPYLSLSQ------NDDAFLSGINYASGGAGILNETGIYF-------------IQR---L 148

Query: 156 PMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSLLVIVVGSNDLFSYYMTDAHND---TTP 215
             + QI+ +    E +  ++G  AA  H + ++  I +GSND  + ++     D    T 
Sbjct: 149 TFNDQINCFKKTKEVIRAKIGDGAANKHVNDAMYFIGLGSNDYVNNFLQPFMADGQQYTH 208

Query: 216 QEYVDLMASTFESELKLLYKYGARKFVATAVPLLGCTPSQRMSIPGEECKDEMNYWAGKY 275
            E+V+L+ ST  ++L  +YK GARK +   +  LGC PSQR+      C + +N W  ++
Sbjct: 209 DEFVELLTSTLHNQLTTIYKLGARKVIFHGLGPLGCIPSQRVKSKTRMCLNRVNEWVLEF 268

Query: 276 NIALRLLLQRLEVDLPGFSYSYADIYAITSDIMQTPSSYGFTEIKAACCGLGPLNALIPC 335
           N   + LL  L   LPG  +S+AD Y    D++  P+ YGF     +CC +      + C
Sbjct: 269 NSRTKKLLIDLNKRLPGAKFSFADTYPAVLDLINNPTHYGFKIANTSCCNVDTSVGGL-C 328

Query: 336 IPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIFN 367
           +P + +C NR D +FWD FHP+   N I+ D++F+
Sbjct: 329 LPNSKMCKNRQDFVFWDAFHPSDSANQILADHLFS 340

BLAST of MC10g_new0239 vs. TAIR 10
Match: AT5G08460.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 224.6 bits (571), Expect = 1.8e-58
Identity = 139/380 (36.58%), Postives = 202/380 (53.16%), Query Frame = 0

Query: 13  FFFLFFLSHLKVMVESSPLIPAIYVFGDSLVDVGNNNYLNILAKADFPHNGVDFPDQQYT 72
           F  +F L+  +   E++ + PA++VFGDSLVD GNNN+LN LA++++   G+DF   Q T
Sbjct: 28  FLVVFVLAGGEDSSETTAMFPAMFVFGDSLVDNGNNNHLNSLARSNYLPYGIDFAGNQPT 87

Query: 73  GRFSNGKVVPDFLAEEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGILNET-- 132
           GRFSNGK + DF+ E +GLP +P ++  VDG          + GVN+AS   GIL ET  
Sbjct: 88  GRFSNGKTIVDFIGELLGLPEIPAFMDTVDG------GVDILHGVNYASAAGGILEETGR 147

Query: 133 RLGFNKVQIKNNIEMVEIERDRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSLLVI 192
            LG               ER     M +Q++ +      +   +  ++ + + +KSL+V+
Sbjct: 148 HLG---------------ER---FSMGRQVENFEKTLMEISRSMRKESVKEYMAKSLVVV 207

Query: 193 VVGSNDLFSYYMTD----AHNDTTPQEYVDLMASTFESELKLLYKYGARKFVATAVPLLG 252
            +G+ND  + Y+      + +   P  + DL+ S F + L  LY  G RKFV   V  LG
Sbjct: 208 SLGNNDYINNYLKPRLFLSSSIYDPTSFADLLLSNFTTHLLELYGKGFRKFVIAGVGPLG 267

Query: 253 CTPSQ---RMSIPGEECKDEMNYWAGKYNIALRLLLQRLEVDLPGFS---YSYADIYAIT 312
           C P Q   + ++PG EC + +N  A  +N  L  L+ RL  D    S   + Y + Y   
Sbjct: 268 CIPDQLAAQAALPG-ECVEAVNEMAELFNNRLVSLVDRLNSDNKTASEAIFVYGNTYGAA 327

Query: 313 SDIMQTPSSYGFTEIKAACCGLGPLNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIV 372
            DI+  P +YGF      CCG+G     I C+PLA  C  R+ H+FWD FHPTQ  N I+
Sbjct: 328 VDILTNPFNYGFEVTDRGCCGVGRNRGEITCLPLAVPCAFRDRHVFWDAFHPTQAFNLII 382

Query: 373 VDYIFNGS-NFTYPINIKEL 380
               FNGS +  YPIN+ +L
Sbjct: 388 ALRAFNGSKSDCYPINLSQL 382

BLAST of MC10g_new0239 vs. TAIR 10
Match: AT5G15720.1 (GDSL-motif lipase 7 )

HSP 1 Score: 215.7 bits (548), Expect = 8.5e-56
Identity = 126/363 (34.71%), Postives = 187/363 (51.52%), Query Frame = 0

Query: 27  ESSPLIPAIYVFGDSLVDVGNNNYLNILAKADFPHNGVDFPDQQYTGRFSNGKVVPDFLA 86
           +  PL PA +VFGDSLVD GNNNY+  LA+A++   G+DF     TGRF NG+ V D+ A
Sbjct: 23  DHQPLAPAFFVFGDSLVDSGNNNYIPTLARANYFPYGIDFGFP--TGRFCNGRTVVDYGA 82

Query: 87  EEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGILNETRLGFNKVQIKNNIEMV 146
             +GLPL+PPYLS       ++   + + GVN+AS  +GIL+ET   +      N     
Sbjct: 83  TYLGLPLVPPYLS------PLSIGQNALRGVNYASAAAGILDETGRHYGARTTFNG---- 142

Query: 147 EIERDRPMPMSQQIDLYSSVYEYVVEELGSDAA--QAHFSKSLLVIVVGSNDLFSYYMTD 206
                       QI  +    E  +     + A  + + +KS++ I +GSND  + Y+  
Sbjct: 143 ------------QISQFEITIELRLRRFFQNPADLRKYLAKSIIGINIGSNDYINNYLMP 202

Query: 207 AHNDTTP----QEYVDLMASTFESELKLLYKYGARKFVATAVPLLGCTPSQRMSIPGEE- 266
               T+     ++Y DL+  T  +++  LY  GARK V      LGC PSQ   + G   
Sbjct: 203 ERYSTSQTYSGEDYADLLIKTLSAQISRLYNLGARKMVLAGSGPLGCIPSQLSMVTGNNT 262

Query: 267 --CKDEMNYWAGKYNIALRLLLQRLEVDLPGFSYSYADIYAITSDIMQTPSSYGFTEIKA 326
             C  ++N     +N  L+ L   L   LPG  + Y +++ +  D++  PS YG      
Sbjct: 263 SGCVTKINNMVSMFNSRLKDLANTLNTTLPGSFFVYQNVFDLFHDMVVNPSRYGLVVSNE 322

Query: 327 ACCGLGPLNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIFNGS-NFTYPINI 380
           ACCG G     + C+PL   C +RN ++FWD FHPT+  N I+    F+ S N++YPI++
Sbjct: 323 ACCGNGRYGGALTCLPLQQPCLDRNQYVFWDAFHPTETANKIIAHNTFSKSANYSYPISV 361

BLAST of MC10g_new0239 vs. TAIR 10
Match: AT1G71250.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 214.2 bits (544), Expect = 2.5e-55
Identity = 125/362 (34.53%), Postives = 191/362 (52.76%), Query Frame = 0

Query: 25  MVESSPLIPAIYVFGDSLVDVGNNNYLNILAKADFPHNGVDFPDQQYTGRFSNGKVVPDF 84
           +V     +PA++V GDSLVD GNNN+L  +A+A+F   G+D  + Q TGRFSNG    D 
Sbjct: 32  LVTGQARVPAMFVLGDSLVDAGNNNFLQTVARANFLPYGIDM-NYQPTGRFSNGLTFIDL 91

Query: 85  LAEEVGLPLLPPYLSLVDGNGTITNATSFITGVNFASGGSGILNETRLGFNKVQIKNNIE 144
           LA  + +P  PP+          T+    + GVN+AS  +GIL+ +   +   +   N +
Sbjct: 92  LARLLEIPSPPPFAD------PTTSGNRILQGVNYASAAAGILDVSGYNYGG-RFSLNQQ 151

Query: 145 MVEIERDRPMPMSQQIDLYSSVYEYVVEELGSDAAQAHFSKSLLVIVVGSNDLFSYYMTD 204
           MV +E      +SQ   + S                 + ++SL+V+V GSND  + Y+  
Sbjct: 152 MVNLE----TTLSQLRTMMS-----------PQNFTDYLARSLVVLVFGSNDYINNYLMP 211

Query: 205 AHNDTT----PQEYVDLMASTFESELKLLYKYGARKFVATAVPLLGCTPSQRMS--IPGE 264
              D++    P ++ +L+ S +  +L  LY  G RK     V  LGC P+QR     P +
Sbjct: 212 NLYDSSIRFRPPDFANLLLSQYARQLLTLYSLGLRKIFIPGVAPLGCIPNQRARGISPPD 271

Query: 265 ECKDEMNYWAGKYNIALRLLLQRLEVDLPGFSYSYADIYAITSDIMQTPSSYGFTEIKAA 324
            C D +N   G +N  L+ L+ +L    PG  Y Y + Y+   DI+  P++YGF+ +  A
Sbjct: 272 RCVDSVNQILGTFNQGLKSLVDQLNQRSPGAIYVYGNTYSAIGDILNNPAAYGFSVVDRA 331

Query: 325 CCGLGPLNALIPCIPLASLCPNRNDHLFWDPFHPTQKVNHIVVDYIFNG-SNFTYPINIK 380
           CCG+G     I C+PL + CPNRN ++FWD FHPTQ  N I+    F G  +  YP+N++
Sbjct: 332 CCGIGRNQGQITCLPLQTPCPNRNQYVFWDAFHPTQTANSILARRAFYGPPSDAYPVNVQ 370

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FIA13.2e-7643.10GDSL esterase/lipase At5g55050 OS=Arabidopsis thaliana OX=3702 GN=At5g55050 PE=2... [more]
O234705.0e-6137.99GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3... [more]
Q9FHQ12.1e-5936.72GDSL esterase/lipase At5g37690 OS=Arabidopsis thaliana OX=3702 GN=At5g37690 PE=2... [more]
Q9FNP22.6e-5736.58GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana OX=3702 GN=At5g08460 PE=2... [more]
Q8LFJ91.2e-5434.71GDSL esterase/lipase 7 OS=Arabidopsis thaliana OX=3702 GN=GLIP7 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022151306.12.37e-268100.00GDSL esterase/lipase At5g55050-like [Momordica charantia][more]
XP_022156042.11.64e-20676.65GDSL esterase/lipase At5g55050-like [Momordica charantia][more]
XP_022151286.13.66e-13756.42GDSL esterase/lipase At5g55050-like [Momordica charantia][more]
XP_038903279.17.28e-13553.87GDSL esterase/lipase At5g55050 [Benincasa hispida][more]
XP_022932219.18.59e-13353.33GDSL esterase/lipase At5g55050 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1DE871.15e-268100.00GDSL esterase/lipase At5g55050-like OS=Momordica charantia OX=3673 GN=LOC1110192... [more]
A0A6J1DQZ97.95e-20776.65GDSL esterase/lipase At5g55050-like OS=Momordica charantia OX=3673 GN=LOC1110230... [more]
A0A6J1DE651.77e-13756.42GDSL esterase/lipase At5g55050-like OS=Momordica charantia OX=3673 GN=LOC1110192... [more]
A0A6J1EWE74.16e-13353.33GDSL esterase/lipase At5g55050 OS=Cucurbita moschata OX=3662 GN=LOC111438588 PE=... [more]
A0A6J1HMQ27.69e-13153.33GDSL esterase/lipase At5g55050 OS=Cucurbita maxima OX=3661 GN=LOC111465054 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT5G55050.12.3e-7743.10GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G37690.11.5e-6036.72SGNH hydrolase-type esterase superfamily protein [more]
AT5G08460.11.8e-5836.58GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G15720.18.5e-5634.71GDSL-motif lipase 7 [more]
AT1G71250.12.5e-5534.53GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 20..369
e-value: 1.5E-78
score: 266.4
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 35..364
e-value: 7.5E-34
score: 118.1
NoneNo IPR availablePANTHERPTHR45648:SF90GDSL-LIKE LIPASE/ACYLHYDROLASE SUPERFAMILY PROTEIN-RELATEDcoord: 12..380
NoneNo IPR availablePANTHERPTHR45648GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)coord: 12..380
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 34..366
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 33..368
e-value: 5.55686E-121
score: 355.385

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g_new0239.1MC10g_new0239.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0016298 lipase activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds