MC10g_new0181 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g_new0181
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	indole-3-acetic acid-amido synthetase GH3.6-like
Location	MC10: 8891403 .. 8894103 (-)
RNA-Seq Expression	MC10g_new0181
Synteny	MC10g_new0181

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CGATAAACAAAGTCAGATAAATGCTTGGTATCGTGGTGGATGATGAATGGTTTTCCAGACGTGGGCTTTCATGTAATGCTTGTTTGCTTTCTAACTCACAGCTGCGCCCTTTCTGTGTCTATAAATACCCATTTTCTTGGCTTCTTTTTCCCCCACGCAGAATCCCATCTTCTCCCTTTTCCAACAAACTCTCTCTCTCTCTTCACAAGAAAGAGAAAAATACAACCAACCCATAAAAACAAACCCAAGAAAAAGAAAAAAATGCCTGAAGCCCCAAAGAGGCGTGTGAACGCCAAGGTTGAAGAAGAAGCTCAGAAGGAGGTGCTGGAGAAAAACAAGGCCACTATCCAATTCATCGAAGACGTCACGGCCAACGCCGACCAAGTCCAGCGCTGCGTCCTCACCGAAATCCTCTCCCGCAACGCCTCCGCCGAGTACTTGACCCGCCACGGCCTCCGCCCCAACTCCTCCCCAGAGACATTCAAAAAAACTATCCCTATCATCTCCTACGACCACATCCACCCTGACATCACCCGCATCGCCAATGGCGACTCCTCCCCCATCCTCTGCTCCTCCCCCATCTCCGAGTTCTTAACCAGGTTCATCTCCAATTCATTTAACTGTTATCAATATCCATGAGATTTTATCTCAAAATCAATTGACAATAAGATGAAGCGTTAAATTATTTAAATCTTGTTCGGTCTCTTGGACTTGGGATGGATTCAGCATCTGAGTCACTCTAAACTTTTCTTTTTTGTTTGTTTGCTTGTGTAGTTCTGGGACTTCCGGTGGCGAGAGGAAGCTGATGCCGACGATCGAGGAGGAGCTGGAGAGGCGCTCGCTGTTGTACAGCTTGCTAATGCCGGTGATGAGCCAGTTCGTTCCAGGGCTGGAAAAGGGCAAAGGAATGTACTTTTTGTTCATAAAAGCCGAGTCCCGAACCCCGGGCGGGCTCGTGGCGCGCCCCGTCTTGACCAGCTACTACAAAAGCTCCCACTTTAAGGACCGTCCTTACGATCCCTACACCAATTACACCAGCCCCAACGAGGCCATTCTCTGCCCTGACTCCTACCAAAGCATGTACGCCCAATTGCTGTGCGGCCTCCGCCAGCGCGCCGAAGTCCTCCGCGTCGGTGCCGTCTTCGCCTCCGGCTTCATCCGCGCCATCCGCTTCCTAGAAAAGCACTGGCAGCTTCTATGCCACGACATCCGGACCGGGACGTTGAATGCACAGATCACGGATCAGGCCGTGCGCGAAGCAGTGATGTCACTCCTGCATAGGCCGGAGCCAGAGCTGGCTGAGTTGATTGAAGGGGAGTGCAAGAAGAGCTCGTGGGCAGGAATTATTAGGAGGCTGTGGCCGAACACCAAATATGTTGATGTGATTGTTACGGGGACAATGTCGCAGTACATTGGGACGCTTGATTATTATAGTAATGGGCTGCCTTTGGTTTGTACCATGTATGCTTCTTCCGAGTGCTATTTTGGCGTCAATCTTAACCCTTTGTGTAAACCAAGTGAAGTTGCTTACACTCTCATCCCTTCCATGGCCTACTTCGAGTTCCTTCCCGTCCAAAGAAATAATAACGCCAATCCCGACTCTACCGATGAGCACGACCTCGTCGATCTTACCGACGTCAAGCTTGGGAAAGAATACGAGCTCGTTGTCACCACTTATGCTGGTAATCCGTTCTCTCTTGCTCTTGAATTATAAAGAGATTGCTCCATTAATTTATCTTAGATTAAAAAATGTAGTACCCAGCTCATGAAGTTAAATTTTTGGGTTTGATTGTAGTGGTTTATTCCTCTTTGGCAAAAAGATAAATAAGATTTTAGATTTTGAAAGTTTTCTTTCTGATCGACAGGATTGTATCGCTACCGAGTGGGCGATATTCTGAGAGTGGCGGGATTCAAGAACAAAGCTCCACAATTCAACTTCATCTGCCGGAAAAACGTGGTTCTAAGCATAGATTCCGACAAGACCGACGAAGTGGAGCTTCAGAACGCGGTGAAGAACTCCGTGAACCACTTGGTGCCGTTCGACGCGACTTTAGCGGAGTACACAAGCTATGCCAACACCTCGACAATTCCAGGGCACTACGTGCTATATTGGGAGGTAAACCAGAAGGGGACGACACCTGTGCCCCCATCAGTGTTCGAAGATTGTTGTCTTACGATCGAGGAGTCGCTGAACAGCGTGTACCGACAAGGGCGGGTATCGGACAAGTCGATCGGGCCGCTGGAGATCAAGGTGGTGGAATGTGGTACGTTTGATAAGCTGATGGACTACGCCATCAGCATGGGAGCTTCAATAAACCAGTACAAGACGCCACGCTGTGTGAAGTATGAACCCATTGTGGAGCTTTTGGACTCGAGAGTGGTTGCCACTTATTTCAGCCCTAAATGCCCCAAGTGGGTCGCTGGCCATAAGCAATGGTGCCAACAGGATTAAATTCAACCTTCAAAACCTTAAACCTTAATTAAATTAGCAGATTTTTATGTGGTTTTTTTTTTTTTGGTTCCATGGTAGCTGCATGCTGTCTTTTTTTCCCTTAATTTCTTGCAAGAGAATAATGTACTTGTATGTTCTTGTGATGATCATGATATGTTAGAAGAATTAGGCAAATCATCTTTCTTGTTAGCTTCTTTTTTCTTTCCCCCATTTCTAATTTGGATATAGATCCTTCTCATGAGTAAGCCC

mRNA sequence

ATGATGAATGGTTTTCCAGACGTGGGCTTTCATGTAATGCTTGTTTGCTTTCTAACTCACAGCTGCGCCCTTTCTGTGTCTATAAATACCCATTTTCTTGGCTTCTTTTTCCCCCACGCAGAATCCCATCTTCTCCCTTTTCCAACAAACTCTCTCTCTCTCTTCACAAGAAAGAGAAAAATACAACCAACCCATAAAAACAAACCCAAGAAAAAGAAAAAAATGCCTGAAGCCCCAAAGAGGCGTGTGAACGCCAAGGTTGAAGAAGAAGCTCAGAAGGAGGTGCTGGAGAAAAACAAGGCCACTATCCAATTCATCGAAGACGTCACGGCCAACGCCGACCAAGTCCAGCGCTGCGTCCTCACCGAAATCCTCTCCCGCAACGCCTCCGCCGAGTACTTGACCCGCCACGGCCTCCGCCCCAACTCCTCCCCAGAGACATTCAAAAAAACTATCCCTATCATCTCCTACGACCACATCCACCCTGACATCACCCGCATCGCCAATGGCGACTCCTCCCCCATCCTCTGCTCCTCCCCCATCTCCGAGTTCTTAACCAGTTCTGGGACTTCCGGTGGCGAGAGGAAGCTGATGCCGACGATCGAGGAGGAGCTGGAGAGGCGCTCGCTGTTGTACAGCTTGCTAATGCCGGTGATGAGCCAGTTCGTTCCAGGGCTGGAAAAGGGCAAAGGAATGTACTTTTTGTTCATAAAAGCCGAGTCCCGAACCCCGGGCGGGCTCGTGGCGCGCCCCGTCTTGACCAGCTACTACAAAAGCTCCCACTTTAAGGACCGTCCTTACGATCCCTACACCAATTACACCAGCCCCAACGAGGCCATTCTCTGCCCTGACTCCTACCAAAGCATGTACGCCCAATTGCTGTGCGGCCTCCGCCAGCGCGCCGAAGTCCTCCGCGTCGGTGCCGTCTTCGCCTCCGGCTTCATCCGCGCCATCCGCTTCCTAGAAAAGCACTGGCAGCTTCTATGCCACGACATCCGGACCGGGACGTTGAATGCACAGATCACGGATCAGGCCGTGCGCGAAGCAGTGATGTCACTCCTGCATAGGCCGGAGCCAGAGCTGGCTGAGTTGATTGAAGGGGAGTGCAAGAAGAGCTCGTGGGCAGGAATTATTAGGAGGCTGTGGCCGAACACCAAATATGTTGATGTGATTGTTACGGGGACAATGTCGCAGTACATTGGGACGCTTGATTATTATAGTAATGGGCTGCCTTTGGTTTGTACCATGTATGCTTCTTCCGAGTGCTATTTTGGCGTCAATCTTAACCCTTTGTGTAAACCAAGTGAAGTTGCTTACACTCTCATCCCTTCCATGGCCTACTTCGAGTTCCTTCCCGTCCAAAGAAATAATAACGCCAATCCCGACTCTACCGATGAGCACGACCTCGTCGATCTTACCGACGTCAAGCTTGGGAAAGAATACGAGCTCGTTGTCACCACTTATGCTGGATTGTATCGCTACCGAGTGGGCGATATTCTGAGAGTGGCGGGATTCAAGAACAAAGCTCCACAATTCAACTTCATCTGCCGGAAAAACGTGGTTCTAAGCATAGATTCCGACAAGACCGACGAAGTGGAGCTTCAGAACGCGGTGAAGAACTCCGTGAACCACTTGGTGCCGTTCGACGCGACTTTAGCGGAGTACACAAGCTATGCCAACACCTCGACAATTCCAGGGCACTACGTGCTATATTGGGAGGTAAACCAGAAGGGGACGACACCTGTGCCCCCATCAGTGTTCGAAGATTGTTGTCTTACGATCGAGGAGTCGCTGAACAGCGTGTACCGACAAGGGCGGGTATCGGACAAGTCGATCGGGCCGCTGGAGATCAAGGTGGTGGAATGTGGTACGTTTGATAAGCTGATGGACTACGCCATCAGCATGGGAGCTTCAATAAACCAGTACAAGACGCCACGCTGTGTGAAGTATGAACCCATTGTGGAGCTTTTGGACTCGAGAGTGGTTGCCACTTATTTCAGCCCTAAATGCCCCAAGTGGGTCGCTGGCCATAAGCAATGGTGCCAACAGGATTAA

Coding sequence (CDS)

ATGATGAATGGTTTTCCAGACGTGGGCTTTCATGTAATGCTTGTTTGCTTTCTAACTCACAGCTGCGCCCTTTCTGTGTCTATAAATACCCATTTTCTTGGCTTCTTTTTCCCCCACGCAGAATCCCATCTTCTCCCTTTTCCAACAAACTCTCTCTCTCTCTTCACAAGAAAGAGAAAAATACAACCAACCCATAAAAACAAACCCAAGAAAAAGAAAAAAATGCCTGAAGCCCCAAAGAGGCGTGTGAACGCCAAGGTTGAAGAAGAAGCTCAGAAGGAGGTGCTGGAGAAAAACAAGGCCACTATCCAATTCATCGAAGACGTCACGGCCAACGCCGACCAAGTCCAGCGCTGCGTCCTCACCGAAATCCTCTCCCGCAACGCCTCCGCCGAGTACTTGACCCGCCACGGCCTCCGCCCCAACTCCTCCCCAGAGACATTCAAAAAAACTATCCCTATCATCTCCTACGACCACATCCACCCTGACATCACCCGCATCGCCAATGGCGACTCCTCCCCCATCCTCTGCTCCTCCCCCATCTCCGAGTTCTTAACCAGTTCTGGGACTTCCGGTGGCGAGAGGAAGCTGATGCCGACGATCGAGGAGGAGCTGGAGAGGCGCTCGCTGTTGTACAGCTTGCTAATGCCGGTGATGAGCCAGTTCGTTCCAGGGCTGGAAAAGGGCAAAGGAATGTACTTTTTGTTCATAAAAGCCGAGTCCCGAACCCCGGGCGGGCTCGTGGCGCGCCCCGTCTTGACCAGCTACTACAAAAGCTCCCACTTTAAGGACCGTCCTTACGATCCCTACACCAATTACACCAGCCCCAACGAGGCCATTCTCTGCCCTGACTCCTACCAAAGCATGTACGCCCAATTGCTGTGCGGCCTCCGCCAGCGCGCCGAAGTCCTCCGCGTCGGTGCCGTCTTCGCCTCCGGCTTCATCCGCGCCATCCGCTTCCTAGAAAAGCACTGGCAGCTTCTATGCCACGACATCCGGACCGGGACGTTGAATGCACAGATCACGGATCAGGCCGTGCGCGAAGCAGTGATGTCACTCCTGCATAGGCCGGAGCCAGAGCTGGCTGAGTTGATTGAAGGGGAGTGCAAGAAGAGCTCGTGGGCAGGAATTATTAGGAGGCTGTGGCCGAACACCAAATATGTTGATGTGATTGTTACGGGGACAATGTCGCAGTACATTGGGACGCTTGATTATTATAGTAATGGGCTGCCTTTGGTTTGTACCATGTATGCTTCTTCCGAGTGCTATTTTGGCGTCAATCTTAACCCTTTGTGTAAACCAAGTGAAGTTGCTTACACTCTCATCCCTTCCATGGCCTACTTCGAGTTCCTTCCCGTCCAAAGAAATAATAACGCCAATCCCGACTCTACCGATGAGCACGACCTCGTCGATCTTACCGACGTCAAGCTTGGGAAAGAATACGAGCTCGTTGTCACCACTTATGCTGGATTGTATCGCTACCGAGTGGGCGATATTCTGAGAGTGGCGGGATTCAAGAACAAAGCTCCACAATTCAACTTCATCTGCCGGAAAAACGTGGTTCTAAGCATAGATTCCGACAAGACCGACGAAGTGGAGCTTCAGAACGCGGTGAAGAACTCCGTGAACCACTTGGTGCCGTTCGACGCGACTTTAGCGGAGTACACAAGCTATGCCAACACCTCGACAATTCCAGGGCACTACGTGCTATATTGGGAGGTAAACCAGAAGGGGACGACACCTGTGCCCCCATCAGTGTTCGAAGATTGTTGTCTTACGATCGAGGAGTCGCTGAACAGCGTGTACCGACAAGGGCGGGTATCGGACAAGTCGATCGGGCCGCTGGAGATCAAGGTGGTGGAATGTGGTACGTTTGATAAGCTGATGGACTACGCCATCAGCATGGGAGCTTCAATAAACCAGTACAAGACGCCACGCTGTGTGAAGTATGAACCCATTGTGGAGCTTTTGGACTCGAGAGTGGTTGCCACTTATTTCAGCCCTAAATGCCCCAAGTGGGTCGCTGGCCATAAGCAATGGTGCCAACAGGATTAA

Protein sequence

MMNGFPDVGFHVMLVCFLTHSCALSVSINTHFLGFFFPHAESHLLPFPTNSLSLFTRKRKIQPTHKNKPKKKKKMPEAPKRRVNAKVEEEAQKEVLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYLTRHGLRPNSSPETFKKTIPIISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLTSYYKSSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGFIRAIRFLEKHWQLLCHDIRTGTLNAQITDQAVREAVMSLLHRPEPELAELIEGECKKSSWAGIIRRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYFEFLPVQRNNNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLAEYTSYANTSTIPGHYVLYWEVNQKGTTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVECGTFDKLMDYAISMGASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPKWVAGHKQWCQQD

Homology

[Close]

BLAST of MC10g_new0181 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 968.4 bits (2502), Expect = 4.3e-281
Identity = 473/614 (77.04%), Postives = 530/614 (86.32%), Query Frame = 0


Query: 75  MPEAPKRRVNAKVEEEAQKEVLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYL 134
           MPEAPK        E + + + EKNK  +QFIEDVT NAD VQR VL EILSRNA  EYL
Sbjct: 1   MPEAPK----IAALEVSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYL 60

Query: 135 TRHGLRPNSSPETFKKTIPIISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGE 194
            RHGL   +  ETFK  +P+++Y+ I P+I RIANGD S +LCS+PISEFLTSSGTSGGE
Sbjct: 61  KRHGLEGRTDRETFKHIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGE 120

Query: 195 RKLMPTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLT 254
           RKLMPTIEEEL+RRSLLYSLLMPVM QFVPGL+KGKGMYFLFIK+ES+TPGGL ARPVLT
Sbjct: 121 RKLMPTIEEELDRRSLLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLT 180

Query: 255 SYYKSSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGF 314
           SYYKSSHFK+RPYDPYTNYTSPN+ ILC DSYQSMY+Q+LCGL Q  EVLRVGAVFASGF
Sbjct: 181 SYYKSSHFKNRPYDPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGF 240

Query: 315 IRAIRFLEKHWQLLCHDIRTGTLNAQITDQAVREAVMSLLHRPEPELAELIEGECKKSSW 374
           IRAI+FLEKHW  L  DIRTGTL+++ITD +VREAV  +L +P+P+LA+ +E EC+K+SW
Sbjct: 241 IRAIKFLEKHWPELARDIRTGTLSSEITDSSVREAVGEIL-KPDPKLADFVESECRKTSW 300

Query: 375 AGIIRRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKP 434
            GII RLWPNTKYVDVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNL PLCKP
Sbjct: 301 QGIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKP 360

Query: 435 SEVAYTLIPSMAYFEFLPVQRNNNANPD--------STDEHDLVDLTDVKLGKEYELVVT 494
           SEV+YTLIP+MAYFEFLPV RN+               ++ +LVDL DVKLG+EYELVVT
Sbjct: 361 SEVSYTLIPNMAYFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVT 420

Query: 495 TYAGLYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLV 554
           TYAGLYRYRVGD+L VAGFKN APQF+FICRKNVVLSIDSDKTDEVELQNAVKN+V HLV
Sbjct: 421 TYAGLYRYRVGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLV 480

Query: 555 PFDATLAEYTSYANTSTIPGHYVLYWEVNQKGTTPVPPSVFEDCCLTIEESLNSVYRQGR 614
           PFDA+L+EYTSYA+TS+IPGHYVL+WE+   G TP+PPSVFEDCCLTIEESLNSVYRQGR
Sbjct: 481 PFDASLSEYTSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGR 540

Query: 615 VSDKSIGPLEIKVVECGTFDKLMDYAISMGASINQYKTPRCVKYEPIVELLDSRVVATYF 674
           VSDKSIGPLEIK+VE GTFDKLMDYAIS+GASINQYKTPRCVK+ PI+ELL+SRVV +YF
Sbjct: 541 VSDKSIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYF 600

Query: 675 SPKCPKWVAGHKQW 681
           SPKCPKW  GHKQW
Sbjct: 601 SPKCPKWSPGHKQW 609

[Close]

BLAST of MC10g_new0181 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 1.3e-277
Identity = 473/614 (77.04%), Postives = 526/614 (85.67%), Query Frame = 0


Query: 75  MPEAPKRRVNAKVEEEAQKEVLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYL 134
           MPEAPK+    +  E     + +KNK  +Q IE++T+NADQVQR VL EIL+RNA  EYL
Sbjct: 1   MPEAPKK----ESLEVFDLTLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYL 60

Query: 135 TRHGLRPNSSPETFKKTIPIISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGE 194
            RH L   +  ETFK  +P+I+Y+ I P+I RIANGD SPIL S PISEFLTSSGTSGGE
Sbjct: 61  RRHDLNGRTDRETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGE 120

Query: 195 RKLMPTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLT 254
           RKLMPTIEEEL+RRSLLYSLLMPVMSQFVPGLE GKGMYFLFIK+ES+TPGGL ARPVLT
Sbjct: 121 RKLMPTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLT 180

Query: 255 SYYKSSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGF 314
           SYYKSSHFK+RPYDPYTNYTSPNE ILC DSYQSMY+Q+LCGL Q  EVLRVGAVFASGF
Sbjct: 181 SYYKSSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGF 240

Query: 315 IRAIRFLEKHWQLLCHDIRTGTLNAQITDQAVREAVMSLLHRPEPELAELIEGECKKSSW 374
           IRAI+FLEKHW  L  DIRTGTL++ ITD +VREAV  +L +P P+LA+ +E ECKKSSW
Sbjct: 241 IRAIKFLEKHWIELVRDIRTGTLSSLITDPSVREAVAKIL-KPSPKLADFVEFECKKSSW 300

Query: 375 AGIIRRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKP 434
            GII RLWPNTKYVDVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNL PLCKP
Sbjct: 301 QGIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKP 360

Query: 435 SEVAYTLIPSMAYFEFLPVQRNNNANPD--------STDEHDLVDLTDVKLGKEYELVVT 494
           SEV+YTLIPSMAYFEFLPV RNN               ++ +LVDL DVKLG+EYELVVT
Sbjct: 361 SEVSYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVT 420

Query: 495 TYAGLYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLV 554
           TYAGL RYRVGD+LRV GFKNKAPQF+FICRKNVVLSIDSDKTDEVELQNAVKN+V HLV
Sbjct: 421 TYAGLCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLV 480

Query: 555 PFDATLAEYTSYANTSTIPGHYVLYWEVNQKGTTPVPPSVFEDCCLTIEESLNSVYRQGR 614
           PFDA+L+EYTSYA+TS+IPGHYVL+WE+   G TP+PPSVFEDCCL +EES N+VYRQGR
Sbjct: 481 PFDASLSEYTSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGR 540

Query: 615 VSDKSIGPLEIKVVECGTFDKLMDYAISMGASINQYKTPRCVKYEPIVELLDSRVVATYF 674
           VSDKSIGPLEIK+VE GTFDKLMDYAIS+GASINQYKTPRCVK+ PI+ELL+SRVV +YF
Sbjct: 541 VSDKSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYF 600

Query: 675 SPKCPKWVAGHKQW 681
           SPKCPKWV GHKQW
Sbjct: 601 SPKCPKWVPGHKQW 609

[Close]

BLAST of MC10g_new0181 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 2.7e-243
Identity = 421/608 (69.24%), Postives = 489/608 (80.43%), Query Frame = 0


Query: 75  MPEAPKRRVNAKVEEEAQKEVLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYL 134
           MPEAP  +        A       +   ++FIE VTANA QVQR VL EIL++NA AEYL
Sbjct: 1   MPEAPTAKT-----APAYGYAPGAHAEALEFIEHVTANAGQVQRRVLGEILAQNAPAEYL 60

Query: 135 TRHGLRPNSSP--ETFKKTIPIISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSG 194
            R+G+ P S    + F++ +P+++Y+ + PDI RIANGD+SPI    PISEFLTSSGTSG
Sbjct: 61  RRYGI-PGSPDVVDAFRRLVPLVTYEGLQPDILRIANGDTSPIFSGKPISEFLTSSGTSG 120

Query: 195 GERKLMPTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPV 254
           GERKLMPTI +E+ RRSLLYSLLMPVMSQ V GL+KGK MY LF+KAESRTPGGL ARPV
Sbjct: 121 GERKLMPTIADEMNRRSLLYSLLMPVMSQSVSGLDKGKAMYLLFVKAESRTPGGLAARPV 180

Query: 255 LTSYYKSSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFAS 314
           LTSYY+S  F DRP DPYT+YTSP+EAILC DSYQSMYAQLLCGL  RA+VLRVGAVFAS
Sbjct: 181 LTSYYRSRQFLDRPRDPYTSYTSPDEAILCVDSYQSMYAQLLCGLVHRADVLRVGAVFAS 240

Query: 315 GFIRAIRFLEKHWQLLCHDIRTGTLNAQITDQAVREAVMSLLHRPEPELAELIEGECKKS 374
           GF+RAI FLEKHW  LCHDIRTG L+ +ITD+ VR+AV  +L R +P LA+ IE EC ++
Sbjct: 241 GFLRAIHFLEKHWARLCHDIRTGELDPEITDRVVRDAVGRVL-RADPALADAIEDECARA 300

Query: 375 SWAGIIRRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLC 434
           SW GIIRRLWP TKY+DVIVTGTMSQYI TL++Y  GLPL CTMYASSECYFG+NLNP+C
Sbjct: 301 SWEGIIRRLWPRTKYIDVIVTGTMSQYIPTLEFYGGGLPLTCTMYASSECYFGLNLNPMC 360

Query: 435 KPSEVAYTLIPSMAYFEFLPVQRNNNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLY 494
           KPS+VAYTLIP+M Y+EFLPV  NN     S    DLVDL DVKLG EYELVVTTY+GLY
Sbjct: 361 KPSDVAYTLIPTMCYYEFLPVNCNNATAEAS--HRDLVDLVDVKLGHEYELVVTTYSGLY 420

Query: 495 RYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATL 554
           RYRVGD+LRVAGFKNKAP F+F+ R+NV LS+DSDKTDE EL  AV  +V HL PF A+L
Sbjct: 421 RYRVGDVLRVAGFKNKAPMFSFVRRQNVALSVDSDKTDETELHAAVSGAVQHLAPFGASL 480

Query: 555 AEYTSYANTSTIPGHYVLYWEVNQKGTTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSI 614
            EYTSYA+ +TIPGHYVL+WE+ + G+T VP SVFE+CCL++EE+LNSVYRQGR  D+SI
Sbjct: 481 VEYTSYADAATIPGHYVLFWEL-RAGSTAVPASVFEECCLSVEEALNSVYRQGRACDRSI 540

Query: 615 GPLEIKVVECGTFDKLMDYAISMGASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPK 674
           GPLEI+VV  GTFDKLMDYAIS GASINQYK PRCV+  P+VELLD+RV   YFSPKCPK
Sbjct: 541 GPLEIRVVAEGTFDKLMDYAISRGASINQYKAPRCVRPGPVVELLDARVQGKYFSPKCPK 598

Query: 675 WVAGHKQW 681
           W  G+KQW
Sbjct: 601 WSPGNKQW 598

[Close]

BLAST of MC10g_new0181 vs. ExPASy Swiss-Prot
Match: Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 2.5e-236
Identity = 416/615 (67.64%), Postives = 487/615 (79.19%), Query Frame = 0


Query: 75  MPEAPKRRVNAKVEEEAQKEVLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYL 134
           MPEA    V+      A   +  +++  ++ IE VT NA Q QR VL EIL++NA AEYL
Sbjct: 1   MPEAVAAAVSPAA--AAATAMCAEHREKLEHIERVTRNAGQEQRRVLEEILAQNAQAEYL 60

Query: 135 TRHGLRPNSSP---ETFKKTIPIISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTS 194
            R G+ P  +P   E F++  P+++Y+ I PD+ RIANGD+SPIL   P+SEFLTSSGTS
Sbjct: 61  RRLGV-PGDAPGADEAFRRLAPLVTYEDILPDVLRIANGDTSPILSGKPVSEFLTSSGTS 120

Query: 195 GGERKLMPTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARP 254
           GGERKLMPTIEEE+ERRS LYSLLMPVMS+ VPGL+KGK MY  F+K+E RTPGGL ARP
Sbjct: 121 GGERKLMPTIEEEMERRSGLYSLLMPVMSRQVPGLDKGKAMYLYFVKSEWRTPGGLPARP 180

Query: 255 VLTSYYKSSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFA 314
           VLTS+Y+S +F +RP+DPYT YTSP+EA+LC D+YQSMYAQL+CGL  RA+VLRVGAVFA
Sbjct: 181 VLTSFYRSRYFLERPHDPYTVYTSPDEAVLCEDAYQSMYAQLICGLVHRADVLRVGAVFA 240

Query: 315 SGFIRAIRFLEKHWQLLCHDIRTGTLNAQITDQAVREAVMSLLHRPEPELAELIEGECKK 374
           SGF+RAIRFLEKHW  LC DIR G L+  +TD AVR AV  +L   +P LA+ IE EC +
Sbjct: 241 SGFLRAIRFLEKHWPSLCRDIRAGELDGGVTDPAVRGAVGRVLRGADPALADAIEAECAR 300

Query: 375 SSWAGIIRRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPL 434
            SW GIIRR+WP+TKY+DVIVTG M+QYI TL++Y  GLPL CTMYASSECYFG+NLNP+
Sbjct: 301 PSWQGIIRRVWPSTKYIDVIVTGAMAQYIPTLEFYGGGLPLACTMYASSECYFGLNLNPM 360

Query: 435 CKPSEVAYTLIPSMAYFEFLPVQRNNNANPDSTDEHD---LVDLTDVKLGKEYELVVTTY 494
           CKPSEVAYTLIP+M YFEFLPV  N+ AN  +  E D   LVDL DVKLG EYELVVTTY
Sbjct: 361 CKPSEVAYTLIPTMCYFEFLPV--NSGANDVAAPEPDHRGLVDLVDVKLGHEYELVVTTY 420

Query: 495 AGLYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPF 554
           +GLYRYRVGD+LRVAGFKN AP F F+ RKNV LSIDSDKTDE EL  AV  +V HL PF
Sbjct: 421 SGLYRYRVGDVLRVAGFKNAAPMFAFVRRKNVALSIDSDKTDEAELHAAVTEAVQHLAPF 480

Query: 555 DATLAEYTSYANT-STIPGHYVLYWEV-NQKGTTPVPPSVFEDCCLTIEESLNSVYRQGR 614
            A+L EYTSYA+T +TIPGHYVL+WE+ +  G TPVP SVFEDCCL +EE LNSVYRQ R
Sbjct: 481 GASLVEYTSYADTATTIPGHYVLFWELRSPAGGTPVPASVFEDCCLAVEEGLNSVYRQCR 540

Query: 615 VSDKSIGPLEIKVVECGTFDKLMDYAISMGASINQYKTPRCVKYEPIVELLDSRVVATYF 674
            +D+SIGPLEI+VV  GTFDKLMDYA+S GASINQYK PRCV+  P+VELLD RV ATYF
Sbjct: 541 AADRSIGPLEIRVVADGTFDKLMDYALSRGASINQYKAPRCVRPGPVVELLDGRVQATYF 600

Query: 675 SPKCPKWVA-GHKQW 681
           SPKCPKW A G+KQW
Sbjct: 601 SPKCPKWCAGGNKQW 610

[Close]

BLAST of MC10g_new0181 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 764.2 bits (1972), Expect = 1.2e-219
Identity = 376/579 (64.94%), Postives = 452/579 (78.07%), Query Frame = 0


Query: 97  EKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYLTRHGLRPNSSPETFKKTIPIIS 156
           EK+   ++FIE++T NAD VQ  +L EIL+RNA  EYL R  L   +  +TFK  IP+I+
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 157 YDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLM 216
           Y+ + P+I RIA+GD SPIL + PISEFLTSSGTS GERKLMPTI+EEL+RR LLYSLLM
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 217 PVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLTSYYKSSHFKDRPYDPYTNYTSP 276
           PVM+ +VPGL+KGKGMYFLF+K+E++TPGGL ARPVLTSYYKS HF+ RPYDPY  YTSP
Sbjct: 137 PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSP 196

Query: 277 NEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGFIRAIRFLEKHWQLLCHDIRTGT 336
           NEAILCPDS+QSMY Q+LCGL  R  VLRVGAVFASG +RAIRFL+ HW    HDI  G 
Sbjct: 197 NEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 256

Query: 337 LNAQITDQAVREAVMSLLHRPEPELAELIEGECKKSSWAGIIRRLWPNTKYVDVIVTGTM 396
           L+++ITD ++R+ +  +L +P+P LAE I  ECK  +W  II R+WPNTKY+DVIVTG M
Sbjct: 257 LDSEITDPSIRQCMSGIL-KPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAM 316

Query: 397 SQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYFEFLPVQRN 456
           +QYI TL+YYS GLP+ CTMYASSECYFG+NLNP+ KPSEV+YT++P+MAYFEF+P+   
Sbjct: 317 AQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGGT 376

Query: 457 NNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFNFIC 516
                        V+L DV +GKEYELVVTTYAGL RYRVGDILRV GF N APQF+F+ 
Sbjct: 377 K-----------AVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVR 436

Query: 517 RKNVVLSIDSDKTDEVELQNAVKNSVNHL-VPFDATLAEYTSYANTSTIPGHYVLYWE-V 576
           RKNV+LSIDSDKTDE ELQ AV+N+ + L     + +AEYTSYA+TSTIPGHYVLYWE +
Sbjct: 437 RKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELL 496

Query: 577 NQKGTTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVECGTFDKLMDYAIS 636
            + G           CCL +EESLNSVYRQ RV+D S+GPLEI+VV  GTF++LMDYAIS
Sbjct: 497 VRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAIS 556

Query: 637 MGASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPKW 674
            GASINQYK PRCV + PIVELLDSRVV+ +FSP  P W
Sbjct: 557 RGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHW 583

[Close]

BLAST of MC10g_new0181 vs. NCBI nr
Match: XP_022142856.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])

HSP 1 Score: 1237 bits (3200), Expect = 0.0
Identity = 610/610 (100.00%), Postives = 610/610 (100.00%), Query Frame = 0


Query: 75  MPEAPKRRVNAKVEEEAQKEVLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYL 134
           MPEAPKRRVNAKVEEEAQKEVLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYL
Sbjct: 1   MPEAPKRRVNAKVEEEAQKEVLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYL 60

Query: 135 TRHGLRPNSSPETFKKTIPIISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGE 194
           TRHGLRPNSSPETFKKTIPIISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGE
Sbjct: 61  TRHGLRPNSSPETFKKTIPIISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGE 120

Query: 195 RKLMPTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLT 254
           RKLMPTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLT
Sbjct: 121 RKLMPTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLT 180

Query: 255 SYYKSSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGF 314
           SYYKSSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGF
Sbjct: 181 SYYKSSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGF 240

Query: 315 IRAIRFLEKHWQLLCHDIRTGTLNAQITDQAVREAVMSLLHRPEPELAELIEGECKKSSW 374
           IRAIRFLEKHWQLLCHDIRTGTLNAQITDQAVREAVMSLLHRPEPELAELIEGECKKSSW
Sbjct: 241 IRAIRFLEKHWQLLCHDIRTGTLNAQITDQAVREAVMSLLHRPEPELAELIEGECKKSSW 300

Query: 375 AGIIRRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKP 434
           AGIIRRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKP
Sbjct: 301 AGIIRRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKP 360

Query: 435 SEVAYTLIPSMAYFEFLPVQRNNNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLYRY 494
           SEVAYTLIPSMAYFEFLPVQRNNNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLYRY
Sbjct: 361 SEVAYTLIPSMAYFEFLPVQRNNNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLYRY 420

Query: 495 RVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLAE 554
           RVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLAE
Sbjct: 421 RVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLAE 480

Query: 555 YTSYANTSTIPGHYVLYWEVNQKGTTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGP 614
           YTSYANTSTIPGHYVLYWEVNQKGTTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGP
Sbjct: 481 YTSYANTSTIPGHYVLYWEVNQKGTTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGP 540

Query: 615 LEIKVVECGTFDKLMDYAISMGASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPKWV 674
           LEIKVVECGTFDKLMDYAISMGASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPKWV
Sbjct: 541 LEIKVVECGTFDKLMDYAISMGASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPKWV 600

Query: 675 AGHKQWCQQD 684
           AGHKQWCQQD
Sbjct: 601 AGHKQWCQQD 610

[Close]

BLAST of MC10g_new0181 vs. NCBI nr
Match: XP_038905526.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Benincasa hispida])

HSP 1 Score: 1047 bits (2707), Expect = 0.0
Identity = 513/592 (86.66%), Postives = 551/592 (93.07%), Query Frame = 0


Query: 95  VLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYLTRHGLRPNSSPETFKKTIPI 154
           +++KNKA +QFIEDVT NA  VQR VL++ILSRN+++EYLT +G RP  SP+TFK +IP+
Sbjct: 14  LVQKNKAILQFIEDVTTNAALVQRQVLSQILSRNSNSEYLTLYG-RP--SPDTFKTSIPL 73

Query: 155 ISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSL 214
           +SY+ I P ++RIANGD SPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSL
Sbjct: 74  VSYEQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSL 133

Query: 215 LMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLTSYYKSSHFKDRPYDPYTNYT 274
           LMPVM+QFVPGLEKGKGMYFLF+KAE++TPGGL+ARPVLTSYYKSSHFKDRPYDPYTNYT
Sbjct: 134 LMPVMTQFVPGLEKGKGMYFLFVKAEAKTPGGLLARPVLTSYYKSSHFKDRPYDPYTNYT 193

Query: 275 SPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGFIRAIRFLEKHWQLLCHDIRT 334
           SPNEAILCPDSYQSMYAQLLCGL  R  VLRVGAVFASGFIRAIRFLEKHWQLLCHDIRT
Sbjct: 194 SPNEAILCPDSYQSMYAQLLCGLCHRLNVLRVGAVFASGFIRAIRFLEKHWQLLCHDIRT 253

Query: 335 GTLNAQITDQAVREAVMSLLHRPEPELAELIEGECKKSSWAGIIRRLWPNTKYVDVIVTG 394
           GTLN+QITDQ VR+AVM +L RP+PELA+ + GEC K SW GII RLWPNTKYVDVIVTG
Sbjct: 254 GTLNSQITDQEVRDAVMLVLRRPDPELADYVHGECCKGSWQGIITRLWPNTKYVDVIVTG 313

Query: 395 TMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYFEFLPVQ 454
           TMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYFEFLPV+
Sbjct: 314 TMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYFEFLPVE 373

Query: 455 RNNNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFNF 514
           RN+  +  S DE  LVDLTDV+LGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFNF
Sbjct: 374 RNHKGDNKSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFNF 433

Query: 515 ICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLAEYTSYANTSTIPGHYVLYWEV 574
           ICRKNVVLSIDSDKTDEVELQNAVKNS NHLVPF+ATLAEYTSYANTSTIPGHYVLYWE+
Sbjct: 434 ICRKNVVLSIDSDKTDEVELQNAVKNSANHLVPFEATLAEYTSYANTSTIPGHYVLYWEL 493

Query: 575 NQKG--TTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVECGTFDKLMDYA 634
           NQKG  TTPVPPSV EDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVE GTFDKLMDYA
Sbjct: 494 NQKGGSTTPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVETGTFDKLMDYA 553

Query: 635 ISMGASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPKWVAGHKQWCQQD 684
           ISMGASINQYKTPRCVK++PIVELLDSRV+A+YFSPKCPKWV GHKQW QQD
Sbjct: 554 ISMGASINQYKTPRCVKFQPIVELLDSRVLASYFSPKCPKWVPGHKQWSQQD 602

[Close]

BLAST of MC10g_new0181 vs. NCBI nr
Match: XP_023520418.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1045 bits (2701), Expect = 0.0
Identity = 518/612 (84.64%), Postives = 561/612 (91.67%), Query Frame = 0


Query: 75  MPEAPKRRVNAKVEEEAQKEVLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYL 134
           MPEAPK   +          +++KN A +QFIEDVT NAD VQR VL++ILSRNA++EYL
Sbjct: 1   MPEAPKTPTSV----SPNYNLVQKNNAILQFIEDVTTNADLVQRQVLSQILSRNATSEYL 60

Query: 135 TRHGLRPNSSPETFKKTIPIISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGE 194
           + HG RP  SP+TFK +IP++SYDHI P I+RIANGD+SPILCSSPISEFLTSSGTSGGE
Sbjct: 61  SLHG-RP--SPDTFKSSIPLVSYDHIQPFISRIANGDTSPILCSSPISEFLTSSGTSGGE 120

Query: 195 RKLMPTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLT 254
           RKLMPTIEEELERRSLLYSLLMP+M+QFVPGLEKGKGMYFLF+KAE+RTPGGL+ARPVLT
Sbjct: 121 RKLMPTIEEELERRSLLYSLLMPIMTQFVPGLEKGKGMYFLFVKAEARTPGGLLARPVLT 180

Query: 255 SYYKSSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGF 314
           SYYKSSHFK+RPYDPYTNYTSPNEAILCPD+YQS+YAQLLCGL  R +VLRVGAVFASGF
Sbjct: 181 SYYKSSHFKNRPYDPYTNYTSPNEAILCPDAYQSIYAQLLCGLCYRLDVLRVGAVFASGF 240

Query: 315 IRAIRFLEKHWQLLCHDIRTGTLNAQITDQAVREAVMSLLHRPEPELAELIEGECKKSSW 374
           IRAIRFLEKHWQLLCHDIRTGTLN+QITDQAVR+AVM +L RP+PELA+ I  +C K  W
Sbjct: 241 IRAIRFLEKHWQLLCHDIRTGTLNSQITDQAVRDAVMLVLRRPDPELADFIHEQCCKGYW 300

Query: 375 AGIIRRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKP 434
            GII RLWPNTKY+DVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKP
Sbjct: 301 KGIITRLWPNTKYLDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKP 360

Query: 435 SEVAYTLIPSMAYFEFLPVQRNNNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLYRY 494
           SEVAYTLIPSMAYFEFLPVQR +N N +S D+  LVDLTDV+LGKEYELVVTTYAGLYRY
Sbjct: 361 SEVAYTLIPSMAYFEFLPVQRTSN-NDESLDDQQLVDLTDVELGKEYELVVTTYAGLYRY 420

Query: 495 RVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLAE 554
           RVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPF+ATLAE
Sbjct: 421 RVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAE 480

Query: 555 YTSYANTSTIPGHYVLYWEVNQKG--TTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSI 614
           YTS+ANTSTIPGHYVLYWE+NQKG  TTPVPPSV EDCCLTIEESLNSVYRQGRV+DKSI
Sbjct: 481 YTSHANTSTIPGHYVLYWELNQKGGSTTPVPPSVLEDCCLTIEESLNSVYRQGRVADKSI 540

Query: 615 GPLEIKVVECGTFDKLMDYAISMGASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPK 674
           GPLEIKVVE GTFDKLMDYAISMGASINQYKTPRCVK++PIVELL+SRVV  YFSPKCPK
Sbjct: 541 GPLEIKVVESGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGCYFSPKCPK 600

Query: 675 WVAGHKQWCQQD 684
           WVAGHKQW QQD
Sbjct: 601 WVAGHKQWSQQD 604

[Close]

BLAST of MC10g_new0181 vs. NCBI nr
Match: XP_022942727.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 1043 bits (2696), Expect = 0.0
Identity = 510/591 (86.29%), Postives = 549/591 (92.89%), Query Frame = 0


Query: 95  VLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYLTRHGLRPNSSPETFKKTIPI 154
           +LEKN A +QFIED+T NA  VQR VL++ILSRNAS+EYLT H  RP  SP+TFK +IP+
Sbjct: 10  LLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHA-RP--SPDTFKSSIPL 69

Query: 155 ISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSL 214
           ISYDHI P ITRIANGD+SPILCSSPISEFLTSSGTSGGERKLMPTI EEL+RRSLLYSL
Sbjct: 70  ISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRRSLLYSL 129

Query: 215 LMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLTSYYKSSHFKDRPYDPYTNYT 274
           LMPVM+QF+PGLEKGKGMYFLF+KAE+RTPGGL+ARPVLTSYYKSSHFKDRPYDPYTNYT
Sbjct: 130 LMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYDPYTNYT 189

Query: 275 SPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGFIRAIRFLEKHWQLLCHDIRT 334
           SPNEAILC DSYQSMYAQ+LCGL  R +VLRVGAVFASGFIRAIRFLEKHWQLLCHDIR+
Sbjct: 190 SPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLCHDIRS 249

Query: 335 GTLNAQITDQAVREAV-MSLLHRPEPELAELIEGECKKSSWAGIIRRLWPNTKYVDVIVT 394
           GTLN+QITDQAVREAV MS+L RP+PEL + IEGEC K SW GII RLWPNTKYVDVIVT
Sbjct: 250 GTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKYVDVIVT 309

Query: 395 GTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYFEFLPV 454
           G MSQYIGTLDYYSNGLPLVC+MYASSECYFG+NLNPLC P+EVAYTLIPSMAYFEFLP+
Sbjct: 310 GAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAYFEFLPL 369

Query: 455 QRNNNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFN 514
           Q N+N       EH LVDLTDV+LGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFN
Sbjct: 370 QTNHNH------EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFN 429

Query: 515 FICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLAEYTSYANTSTIPGHYVLYWE 574
           F+CRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATL +YTSYANTSTIPGHYV+YWE
Sbjct: 430 FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE 489

Query: 575 VNQKGTTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVECGTFDKLMDYAI 634
           VNQKG+T VPPSVFEDCCLT+EESLNSVYRQGRVSDKSIGPLEIKVVE GTFDKLMDYAI
Sbjct: 490 VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 549

Query: 635 SMGASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPKWVAGHKQWCQQD 684
           +MGASINQYKTPRCVK+EPIV+LL+SRVV +YFSPKCPKWVAGHKQWCQQD
Sbjct: 550 TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQD 591

[Close]

BLAST of MC10g_new0181 vs. NCBI nr
Match: XP_022985190.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima])

HSP 1 Score: 1041 bits (2691), Expect = 0.0
Identity = 516/612 (84.31%), Postives = 559/612 (91.34%), Query Frame = 0


Query: 75  MPEAPKRRVNAKVEEEAQKEVLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYL 134
           MPEAPK   +          +++KN A +QFIEDVT NAD VQR VL++ILSRNA++EYL
Sbjct: 1   MPEAPKTATSV----SPNYNLVQKNNAILQFIEDVTTNADLVQRQVLSQILSRNATSEYL 60

Query: 135 TRHGLRPNSSPETFKKTIPIISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGE 194
           + HG RP  SP+TFK +IP++SYDHI P I+RIANGD+SPILCSSPISEFLTSSGTSGGE
Sbjct: 61  SLHG-RP--SPDTFKSSIPLVSYDHIQPFISRIANGDTSPILCSSPISEFLTSSGTSGGE 120

Query: 195 RKLMPTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLT 254
           RKLMPTIEEELERRSLLYSLLMP+M+QFVPGLEKGKGMYFLF+KAE+RTPGGL+ARPVLT
Sbjct: 121 RKLMPTIEEELERRSLLYSLLMPIMTQFVPGLEKGKGMYFLFVKAEARTPGGLLARPVLT 180

Query: 255 SYYKSSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGF 314
           SYYKSSHFK+RPYDPYTNYTSPNEAILCPD+YQS+YAQLLCGL  R +VLRVGAVFASGF
Sbjct: 181 SYYKSSHFKNRPYDPYTNYTSPNEAILCPDAYQSIYAQLLCGLCYRLDVLRVGAVFASGF 240

Query: 315 IRAIRFLEKHWQLLCHDIRTGTLNAQITDQAVREAVMSLLHRPEPELAELIEGECKKSSW 374
           IRAIRFLEKHWQLLCHDIRTGTLN+QITDQAVR+AVM +L RP+PELA+ I  +C K  W
Sbjct: 241 IRAIRFLEKHWQLLCHDIRTGTLNSQITDQAVRDAVMLVLRRPDPELADFIHEQCCKGFW 300

Query: 375 AGIIRRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKP 434
            GII RLWPNTKY+DVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKP
Sbjct: 301 KGIITRLWPNTKYLDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKP 360

Query: 435 SEVAYTLIPSMAYFEFLPVQRNNNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLYRY 494
           SEVAYTLIPSMAYFEFLPVQR +N N +S D+  LVDLTDV+LGKEYELVVTTYAGLYRY
Sbjct: 361 SEVAYTLIPSMAYFEFLPVQRTSN-NDESLDDQQLVDLTDVELGKEYELVVTTYAGLYRY 420

Query: 495 RVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLAE 554
           RVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVK SVNHLVPF+ATLAE
Sbjct: 421 RVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKKSVNHLVPFEATLAE 480

Query: 555 YTSYANTSTIPGHYVLYWEVNQKG--TTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSI 614
           YTS+ANTSTIPGHYVLYWE+NQKG   TPVPPSV EDCCLTIEESLNSVYRQGRV+DKSI
Sbjct: 481 YTSHANTSTIPGHYVLYWELNQKGGSATPVPPSVLEDCCLTIEESLNSVYRQGRVADKSI 540

Query: 615 GPLEIKVVECGTFDKLMDYAISMGASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPK 674
           GPLEIKVVE GTFDKLMDYAISMGASINQYKTPRCVK++PIVELL+SRVV  YFSPKCPK
Sbjct: 541 GPLEIKVVESGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGCYFSPKCPK 600

Query: 675 WVAGHKQWCQQD 684
           WVAGHKQW QQD
Sbjct: 601 WVAGHKQWSQQD 604

[Close]

BLAST of MC10g_new0181 vs. ExPASy TrEMBL
Match: A0A6J1CNF7 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111012866 PE=3 SV=1)

HSP 1 Score: 1237 bits (3200), Expect = 0.0
Identity = 610/610 (100.00%), Postives = 610/610 (100.00%), Query Frame = 0


Query: 75  MPEAPKRRVNAKVEEEAQKEVLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYL 134
           MPEAPKRRVNAKVEEEAQKEVLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYL
Sbjct: 1   MPEAPKRRVNAKVEEEAQKEVLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYL 60

Query: 135 TRHGLRPNSSPETFKKTIPIISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGE 194
           TRHGLRPNSSPETFKKTIPIISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGE
Sbjct: 61  TRHGLRPNSSPETFKKTIPIISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGE 120

Query: 195 RKLMPTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLT 254
           RKLMPTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLT
Sbjct: 121 RKLMPTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLT 180

Query: 255 SYYKSSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGF 314
           SYYKSSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGF
Sbjct: 181 SYYKSSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGF 240

Query: 315 IRAIRFLEKHWQLLCHDIRTGTLNAQITDQAVREAVMSLLHRPEPELAELIEGECKKSSW 374
           IRAIRFLEKHWQLLCHDIRTGTLNAQITDQAVREAVMSLLHRPEPELAELIEGECKKSSW
Sbjct: 241 IRAIRFLEKHWQLLCHDIRTGTLNAQITDQAVREAVMSLLHRPEPELAELIEGECKKSSW 300

Query: 375 AGIIRRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKP 434
           AGIIRRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKP
Sbjct: 301 AGIIRRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKP 360

Query: 435 SEVAYTLIPSMAYFEFLPVQRNNNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLYRY 494
           SEVAYTLIPSMAYFEFLPVQRNNNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLYRY
Sbjct: 361 SEVAYTLIPSMAYFEFLPVQRNNNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLYRY 420

Query: 495 RVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLAE 554
           RVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLAE
Sbjct: 421 RVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLAE 480

Query: 555 YTSYANTSTIPGHYVLYWEVNQKGTTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGP 614
           YTSYANTSTIPGHYVLYWEVNQKGTTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGP
Sbjct: 481 YTSYANTSTIPGHYVLYWEVNQKGTTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGP 540

Query: 615 LEIKVVECGTFDKLMDYAISMGASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPKWV 674
           LEIKVVECGTFDKLMDYAISMGASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPKWV
Sbjct: 541 LEIKVVECGTFDKLMDYAISMGASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPKWV 600

Query: 675 AGHKQWCQQD 684
           AGHKQWCQQD
Sbjct: 601 AGHKQWCQQD 610

[Close]

BLAST of MC10g_new0181 vs. ExPASy TrEMBL
Match: A0A6J1FR29 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111447677 PE=3 SV=1)

HSP 1 Score: 1043 bits (2696), Expect = 0.0
Identity = 510/591 (86.29%), Postives = 549/591 (92.89%), Query Frame = 0


Query: 95  VLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYLTRHGLRPNSSPETFKKTIPI 154
           +LEKN A +QFIED+T NA  VQR VL++ILSRNAS+EYLT H  RP  SP+TFK +IP+
Sbjct: 10  LLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHA-RP--SPDTFKSSIPL 69

Query: 155 ISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSL 214
           ISYDHI P ITRIANGD+SPILCSSPISEFLTSSGTSGGERKLMPTI EEL+RRSLLYSL
Sbjct: 70  ISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRRSLLYSL 129

Query: 215 LMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLTSYYKSSHFKDRPYDPYTNYT 274
           LMPVM+QF+PGLEKGKGMYFLF+KAE+RTPGGL+ARPVLTSYYKSSHFKDRPYDPYTNYT
Sbjct: 130 LMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYDPYTNYT 189

Query: 275 SPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGFIRAIRFLEKHWQLLCHDIRT 334
           SPNEAILC DSYQSMYAQ+LCGL  R +VLRVGAVFASGFIRAIRFLEKHWQLLCHDIR+
Sbjct: 190 SPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLCHDIRS 249

Query: 335 GTLNAQITDQAVREAV-MSLLHRPEPELAELIEGECKKSSWAGIIRRLWPNTKYVDVIVT 394
           GTLN+QITDQAVREAV MS+L RP+PEL + IEGEC K SW GII RLWPNTKYVDVIVT
Sbjct: 250 GTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKYVDVIVT 309

Query: 395 GTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYFEFLPV 454
           G MSQYIGTLDYYSNGLPLVC+MYASSECYFG+NLNPLC P+EVAYTLIPSMAYFEFLP+
Sbjct: 310 GAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAYFEFLPL 369

Query: 455 QRNNNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFN 514
           Q N+N       EH LVDLTDV+LGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFN
Sbjct: 370 QTNHNH------EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFN 429

Query: 515 FICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLAEYTSYANTSTIPGHYVLYWE 574
           F+CRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATL +YTSYANTSTIPGHYV+YWE
Sbjct: 430 FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE 489

Query: 575 VNQKGTTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVECGTFDKLMDYAI 634
           VNQKG+T VPPSVFEDCCLT+EESLNSVYRQGRVSDKSIGPLEIKVVE GTFDKLMDYAI
Sbjct: 490 VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 549

Query: 635 SMGASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPKWVAGHKQWCQQD 684
           +MGASINQYKTPRCVK+EPIV+LL+SRVV +YFSPKCPKWVAGHKQWCQQD
Sbjct: 550 TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQD 591

[Close]

BLAST of MC10g_new0181 vs. ExPASy TrEMBL
Match: A0A6J1JAP2 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=LOC111483268 PE=3 SV=1)

HSP 1 Score: 1041 bits (2691), Expect = 0.0
Identity = 516/612 (84.31%), Postives = 559/612 (91.34%), Query Frame = 0


Query: 75  MPEAPKRRVNAKVEEEAQKEVLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYL 134
           MPEAPK   +          +++KN A +QFIEDVT NAD VQR VL++ILSRNA++EYL
Sbjct: 1   MPEAPKTATSV----SPNYNLVQKNNAILQFIEDVTTNADLVQRQVLSQILSRNATSEYL 60

Query: 135 TRHGLRPNSSPETFKKTIPIISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGE 194
           + HG RP  SP+TFK +IP++SYDHI P I+RIANGD+SPILCSSPISEFLTSSGTSGGE
Sbjct: 61  SLHG-RP--SPDTFKSSIPLVSYDHIQPFISRIANGDTSPILCSSPISEFLTSSGTSGGE 120

Query: 195 RKLMPTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLT 254
           RKLMPTIEEELERRSLLYSLLMP+M+QFVPGLEKGKGMYFLF+KAE+RTPGGL+ARPVLT
Sbjct: 121 RKLMPTIEEELERRSLLYSLLMPIMTQFVPGLEKGKGMYFLFVKAEARTPGGLLARPVLT 180

Query: 255 SYYKSSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGF 314
           SYYKSSHFK+RPYDPYTNYTSPNEAILCPD+YQS+YAQLLCGL  R +VLRVGAVFASGF
Sbjct: 181 SYYKSSHFKNRPYDPYTNYTSPNEAILCPDAYQSIYAQLLCGLCYRLDVLRVGAVFASGF 240

Query: 315 IRAIRFLEKHWQLLCHDIRTGTLNAQITDQAVREAVMSLLHRPEPELAELIEGECKKSSW 374
           IRAIRFLEKHWQLLCHDIRTGTLN+QITDQAVR+AVM +L RP+PELA+ I  +C K  W
Sbjct: 241 IRAIRFLEKHWQLLCHDIRTGTLNSQITDQAVRDAVMLVLRRPDPELADFIHEQCCKGFW 300

Query: 375 AGIIRRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKP 434
            GII RLWPNTKY+DVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKP
Sbjct: 301 KGIITRLWPNTKYLDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKP 360

Query: 435 SEVAYTLIPSMAYFEFLPVQRNNNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLYRY 494
           SEVAYTLIPSMAYFEFLPVQR +N N +S D+  LVDLTDV+LGKEYELVVTTYAGLYRY
Sbjct: 361 SEVAYTLIPSMAYFEFLPVQRTSN-NDESLDDQQLVDLTDVELGKEYELVVTTYAGLYRY 420

Query: 495 RVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLAE 554
           RVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVK SVNHLVPF+ATLAE
Sbjct: 421 RVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKKSVNHLVPFEATLAE 480

Query: 555 YTSYANTSTIPGHYVLYWEVNQKG--TTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSI 614
           YTS+ANTSTIPGHYVLYWE+NQKG   TPVPPSV EDCCLTIEESLNSVYRQGRV+DKSI
Sbjct: 481 YTSHANTSTIPGHYVLYWELNQKGGSATPVPPSVLEDCCLTIEESLNSVYRQGRVADKSI 540

Query: 615 GPLEIKVVECGTFDKLMDYAISMGASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPK 674
           GPLEIKVVE GTFDKLMDYAISMGASINQYKTPRCVK++PIVELL+SRVV  YFSPKCPK
Sbjct: 541 GPLEIKVVESGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGCYFSPKCPK 600

Query: 675 WVAGHKQWCQQD 684
           WVAGHKQW QQD
Sbjct: 601 WVAGHKQWSQQD 604

[Close]

BLAST of MC10g_new0181 vs. ExPASy TrEMBL
Match: A0A5D3BYD3 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G00850 PE=3 SV=1)

HSP 1 Score: 1040 bits (2690), Expect = 0.0
Identity = 513/594 (86.36%), Postives = 554/594 (93.27%), Query Frame = 0


Query: 94  EVLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYLTRHGLRPNSSPETFKKTIP 153
           ++++KNKA +QFIEDVT NA  VQR VL++ILS+N+++EYLT +G RP  SP+TFK +IP
Sbjct: 14  QLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYG-RP--SPDTFKTSIP 73

Query: 154 IISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYS 213
           ++SYD I P ++RIANGD SPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYS
Sbjct: 74  LVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYS 133

Query: 214 LLMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLTSYYKSSHFKDRPYDPYTNY 273
           LLMPVMSQFVPGLEKGKGMYFLFIKAE++TPGGL+ARPVLTSYYKSSHFK+RPYDPYTNY
Sbjct: 134 LLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFKERPYDPYTNY 193

Query: 274 TSPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGFIRAIRFLEKHWQLLCHDIR 333
           TSPNEAILCPDSYQSMYAQLLCGL  R +VLRVGAVFASGFIRAIRFLEKHWQLLCHDIR
Sbjct: 194 TSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLCHDIR 253

Query: 334 TGTLNAQITDQAVREAVM-SLLHRPEPELAELIEGECKKSSWAGIIRRLWPNTKYVDVIV 393
           TGTLN+QITDQAVR+AVM S+L RP+PELA+ I GEC K SW GII RLWPNTKYVDVIV
Sbjct: 254 TGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLWPNTKYVDVIV 313

Query: 394 TGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYFEFLP 453
           TGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYFEFLP
Sbjct: 314 TGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYFEFLP 373

Query: 454 VQRNNNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQF 513
           ++R++N + +S DE  LVDLTDV+LGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQF
Sbjct: 374 IERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQF 433

Query: 514 NFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLAEYTSYANTSTIPGHYVLYW 573
           NFICRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPF+ATLAEYTSYANTSTIPGHYVLYW
Sbjct: 434 NFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTSTIPGHYVLYW 493

Query: 574 EVNQK--GTTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVECGTFDKLMD 633
           EVNQK    TPVPPSV EDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVE GTFDKLMD
Sbjct: 494 EVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVENGTFDKLMD 553

Query: 634 YAISMGASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPKWVAGHKQWCQQD 684
           YAISMGASINQYKTPRCVK++PIVELL+SRVV +YFSPKCPKWV GHKQW QQD
Sbjct: 554 YAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQWSQQD 604

[Close]

BLAST of MC10g_new0181 vs. ExPASy TrEMBL
Match: A0A1S3BTN3 (indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103493400 PE=3 SV=1)

HSP 1 Score: 1040 bits (2690), Expect = 0.0
Identity = 513/594 (86.36%), Postives = 554/594 (93.27%), Query Frame = 0


Query: 94  EVLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYLTRHGLRPNSSPETFKKTIP 153
           ++++KNKA +QFIEDVT NA  VQR VL++ILS+N+++EYLT +G RP  SP+TFK +IP
Sbjct: 14  QLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYG-RP--SPDTFKTSIP 73

Query: 154 IISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYS 213
           ++SYD I P ++RIANGD SPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYS
Sbjct: 74  LVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYS 133

Query: 214 LLMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLTSYYKSSHFKDRPYDPYTNY 273
           LLMPVMSQFVPGLEKGKGMYFLFIKAE++TPGGL+ARPVLTSYYKSSHFK+RPYDPYTNY
Sbjct: 134 LLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFKERPYDPYTNY 193

Query: 274 TSPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGFIRAIRFLEKHWQLLCHDIR 333
           TSPNEAILCPDSYQSMYAQLLCGL  R +VLRVGAVFASGFIRAIRFLEKHWQLLCHDIR
Sbjct: 194 TSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLCHDIR 253

Query: 334 TGTLNAQITDQAVREAVM-SLLHRPEPELAELIEGECKKSSWAGIIRRLWPNTKYVDVIV 393
           TGTLN+QITDQAVR+AVM S+L RP+PELA+ I GEC K SW GII RLWPNTKYVDVIV
Sbjct: 254 TGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLWPNTKYVDVIV 313

Query: 394 TGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYFEFLP 453
           TGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYFEFLP
Sbjct: 314 TGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYFEFLP 373

Query: 454 VQRNNNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQF 513
           ++R++N + +S DE  LVDLTDV+LGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQF
Sbjct: 374 IERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQF 433

Query: 514 NFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLAEYTSYANTSTIPGHYVLYW 573
           NFICRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPF+ATLAEYTSYANTSTIPGHYVLYW
Sbjct: 434 NFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTSTIPGHYVLYW 493

Query: 574 EVNQK--GTTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVECGTFDKLMD 633
           EVNQK    TPVPPSV EDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVE GTFDKLMD
Sbjct: 494 EVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVENGTFDKLMD 553

Query: 634 YAISMGASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPKWVAGHKQWCQQD 684
           YAISMGASINQYKTPRCVK++PIVELL+SRVV +YFSPKCPKWV GHKQW QQD
Sbjct: 554 YAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQWSQQD 604

[Close]

BLAST of MC10g_new0181 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 968.4 bits (2502), Expect = 3.1e-282
Identity = 473/614 (77.04%), Postives = 530/614 (86.32%), Query Frame = 0


Query: 75  MPEAPKRRVNAKVEEEAQKEVLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYL 134
           MPEAPK        E + + + EKNK  +QFIEDVT NAD VQR VL EILSRNA  EYL
Sbjct: 1   MPEAPK----IAALEVSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYL 60

Query: 135 TRHGLRPNSSPETFKKTIPIISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGE 194
            RHGL   +  ETFK  +P+++Y+ I P+I RIANGD S +LCS+PISEFLTSSGTSGGE
Sbjct: 61  KRHGLEGRTDRETFKHIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGE 120

Query: 195 RKLMPTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLT 254
           RKLMPTIEEEL+RRSLLYSLLMPVM QFVPGL+KGKGMYFLFIK+ES+TPGGL ARPVLT
Sbjct: 121 RKLMPTIEEELDRRSLLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLT 180

Query: 255 SYYKSSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGF 314
           SYYKSSHFK+RPYDPYTNYTSPN+ ILC DSYQSMY+Q+LCGL Q  EVLRVGAVFASGF
Sbjct: 181 SYYKSSHFKNRPYDPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGF 240

Query: 315 IRAIRFLEKHWQLLCHDIRTGTLNAQITDQAVREAVMSLLHRPEPELAELIEGECKKSSW 374
           IRAI+FLEKHW  L  DIRTGTL+++ITD +VREAV  +L +P+P+LA+ +E EC+K+SW
Sbjct: 241 IRAIKFLEKHWPELARDIRTGTLSSEITDSSVREAVGEIL-KPDPKLADFVESECRKTSW 300

Query: 375 AGIIRRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKP 434
            GII RLWPNTKYVDVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNL PLCKP
Sbjct: 301 QGIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKP 360

Query: 435 SEVAYTLIPSMAYFEFLPVQRNNNANPD--------STDEHDLVDLTDVKLGKEYELVVT 494
           SEV+YTLIP+MAYFEFLPV RN+               ++ +LVDL DVKLG+EYELVVT
Sbjct: 361 SEVSYTLIPNMAYFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVT 420

Query: 495 TYAGLYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLV 554
           TYAGLYRYRVGD+L VAGFKN APQF+FICRKNVVLSIDSDKTDEVELQNAVKN+V HLV
Sbjct: 421 TYAGLYRYRVGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLV 480

Query: 555 PFDATLAEYTSYANTSTIPGHYVLYWEVNQKGTTPVPPSVFEDCCLTIEESLNSVYRQGR 614
           PFDA+L+EYTSYA+TS+IPGHYVL+WE+   G TP+PPSVFEDCCLTIEESLNSVYRQGR
Sbjct: 481 PFDASLSEYTSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGR 540

Query: 615 VSDKSIGPLEIKVVECGTFDKLMDYAISMGASINQYKTPRCVKYEPIVELLDSRVVATYF 674
           VSDKSIGPLEIK+VE GTFDKLMDYAIS+GASINQYKTPRCVK+ PI+ELL+SRVV +YF
Sbjct: 541 VSDKSIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYF 600

Query: 675 SPKCPKWVAGHKQW 681
           SPKCPKW  GHKQW
Sbjct: 601 SPKCPKWSPGHKQW 609

[Close]

BLAST of MC10g_new0181 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 956.8 bits (2472), Expect = 9.2e-279
Identity = 473/614 (77.04%), Postives = 526/614 (85.67%), Query Frame = 0


Query: 75  MPEAPKRRVNAKVEEEAQKEVLEKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYL 134
           MPEAPK+    +  E     + +KNK  +Q IE++T+NADQVQR VL EIL+RNA  EYL
Sbjct: 1   MPEAPKK----ESLEVFDLTLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYL 60

Query: 135 TRHGLRPNSSPETFKKTIPIISYDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGE 194
            RH L   +  ETFK  +P+I+Y+ I P+I RIANGD SPIL S PISEFLTSSGTSGGE
Sbjct: 61  RRHDLNGRTDRETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGE 120

Query: 195 RKLMPTIEEELERRSLLYSLLMPVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLT 254
           RKLMPTIEEEL+RRSLLYSLLMPVMSQFVPGLE GKGMYFLFIK+ES+TPGGL ARPVLT
Sbjct: 121 RKLMPTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLT 180

Query: 255 SYYKSSHFKDRPYDPYTNYTSPNEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGF 314
           SYYKSSHFK+RPYDPYTNYTSPNE ILC DSYQSMY+Q+LCGL Q  EVLRVGAVFASGF
Sbjct: 181 SYYKSSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGF 240

Query: 315 IRAIRFLEKHWQLLCHDIRTGTLNAQITDQAVREAVMSLLHRPEPELAELIEGECKKSSW 374
           IRAI+FLEKHW  L  DIRTGTL++ ITD +VREAV  +L +P P+LA+ +E ECKKSSW
Sbjct: 241 IRAIKFLEKHWIELVRDIRTGTLSSLITDPSVREAVAKIL-KPSPKLADFVEFECKKSSW 300

Query: 375 AGIIRRLWPNTKYVDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKP 434
            GII RLWPNTKYVDVIVTGTMSQYI TLDYYSNGLPLVCTMYASSECYFGVNL PLCKP
Sbjct: 301 QGIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKP 360

Query: 435 SEVAYTLIPSMAYFEFLPVQRNNNANPD--------STDEHDLVDLTDVKLGKEYELVVT 494
           SEV+YTLIPSMAYFEFLPV RNN               ++ +LVDL DVKLG+EYELVVT
Sbjct: 361 SEVSYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVT 420

Query: 495 TYAGLYRYRVGDILRVAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLV 554
           TYAGL RYRVGD+LRV GFKNKAPQF+FICRKNVVLSIDSDKTDEVELQNAVKN+V HLV
Sbjct: 421 TYAGLCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLV 480

Query: 555 PFDATLAEYTSYANTSTIPGHYVLYWEVNQKGTTPVPPSVFEDCCLTIEESLNSVYRQGR 614
           PFDA+L+EYTSYA+TS+IPGHYVL+WE+   G TP+PPSVFEDCCL +EES N+VYRQGR
Sbjct: 481 PFDASLSEYTSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGR 540

Query: 615 VSDKSIGPLEIKVVECGTFDKLMDYAISMGASINQYKTPRCVKYEPIVELLDSRVVATYF 674
           VSDKSIGPLEIK+VE GTFDKLMDYAIS+GASINQYKTPRCVK+ PI+ELL+SRVV +YF
Sbjct: 541 VSDKSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYF 600

Query: 675 SPKCPKWVAGHKQW 681
           SPKCPKWV GHKQW
Sbjct: 601 SPKCPKWVPGHKQW 609

[Close]

BLAST of MC10g_new0181 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 764.2 bits (1972), Expect = 8.8e-221
Identity = 376/579 (64.94%), Postives = 452/579 (78.07%), Query Frame = 0


Query: 97  EKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYLTRHGLRPNSSPETFKKTIPIIS 156
           EK+   ++FIE++T NAD VQ  +L EIL+RNA  EYL R  L   +  +TFK  IP+I+
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 157 YDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLM 216
           Y+ + P+I RIA+GD SPIL + PISEFLTSSGTS GERKLMPTI+EEL+RR LLYSLLM
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 217 PVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLTSYYKSSHFKDRPYDPYTNYTSP 276
           PVM+ +VPGL+KGKGMYFLF+K+E++TPGGL ARPVLTSYYKS HF+ RPYDPY  YTSP
Sbjct: 137 PVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSP 196

Query: 277 NEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGFIRAIRFLEKHWQLLCHDIRTGT 336
           NEAILCPDS+QSMY Q+LCGL  R  VLRVGAVFASG +RAIRFL+ HW    HDI  G 
Sbjct: 197 NEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGC 256

Query: 337 LNAQITDQAVREAVMSLLHRPEPELAELIEGECKKSSWAGIIRRLWPNTKYVDVIVTGTM 396
           L+++ITD ++R+ +  +L +P+P LAE I  ECK  +W  II R+WPNTKY+DVIVTG M
Sbjct: 257 LDSEITDPSIRQCMSGIL-KPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAM 316

Query: 397 SQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYFEFLPVQRN 456
           +QYI TL+YYS GLP+ CTMYASSECYFG+NLNP+ KPSEV+YT++P+MAYFEF+P+   
Sbjct: 317 AQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGGT 376

Query: 457 NNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFNFIC 516
                        V+L DV +GKEYELVVTTYAGL RYRVGDILRV GF N APQF+F+ 
Sbjct: 377 K-----------AVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVR 436

Query: 517 RKNVVLSIDSDKTDEVELQNAVKNSVNHL-VPFDATLAEYTSYANTSTIPGHYVLYWE-V 576
           RKNV+LSIDSDKTDE ELQ AV+N+ + L     + +AEYTSYA+TSTIPGHYVLYWE +
Sbjct: 437 RKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELL 496

Query: 577 NQKGTTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVECGTFDKLMDYAIS 636
            + G           CCL +EESLNSVYRQ RV+D S+GPLEI+VV  GTF++LMDYAIS
Sbjct: 497 VRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAIS 556

Query: 637 MGASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPKW 674
            GASINQYK PRCV + PIVELLDSRVV+ +FSP  P W
Sbjct: 557 RGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHW 583

[Close]

BLAST of MC10g_new0181 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 762.7 bits (1968), Expect = 2.5e-220
Identity = 370/580 (63.79%), Postives = 453/580 (78.10%), Query Frame = 0


Query: 97  EKNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYLTRHGLRPNSSPETFKKTIPIIS 156
           EK+   ++FIE++T N D VQ  VL EIL+RN++ EYL R  L      +TFK  +P+++
Sbjct: 18  EKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVT 77

Query: 157 YDHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLM 216
           Y+ + P+I RI+NGD SPIL S PI+EFLTSSGTS GERKLMPTIEE+L+RR LLYSLLM
Sbjct: 78  YEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLM 137

Query: 217 PVMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLTSYYKSSHFKDRPYDPYTNYTSP 276
           PVM+ +VPGL+KGKG+YFLF+K+ES+T GGL ARPVLTSYYKS HFK RPYDPY  YTSP
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSP 197

Query: 277 NEAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGFIRAIRFLEKHWQLLCHDIRTGT 336
           NEAILC DS QSMYAQ+LCGL  R EVLR+GAVFASG +RAI FL+ +W+ L  DI TGT
Sbjct: 198 NEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGT 257

Query: 337 LNAQITDQAVREAVMSLLHRPEPELAELIEGECKKSSWAGIIRRLWPNTKYVDVIVTGTM 396
           L+++I D A++  +  +L +P+ ELAE + G C + +W GII ++WPNTKY+DVIVTG M
Sbjct: 258 LSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAM 317

Query: 397 SQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYFEFLPVQRN 456
           +QYI TL+YYS GLP+ CTMYASSE YFG+NL P+CKPSEV+YT++P+MAYFEFLP   +
Sbjct: 318 AQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHNHD 377

Query: 457 -NNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFNFI 516
            + A   S DE  LV+L +V++GKEYELV+TTYAGLYRYRVGDILRV GF N APQF FI
Sbjct: 378 GDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFI 437

Query: 517 CRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLAEYTSYANTSTIPGHYVLYWEV- 576
            RKNV+LS++SDKTDE ELQ AV+N+          + EYTSYA T TIPGHYV+YWE+ 
Sbjct: 438 RRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVIYWELL 497

Query: 577 -NQKGTTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVECGTFDKLMDYAI 636
              +    +   V   CCL +EESLNSVYRQ RV+DKSIGPLEI+VV  GTF++LMDYAI
Sbjct: 498 GRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEELMDYAI 557

Query: 637 SMGASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPKW 674
           S GASINQYK PRCV + PI+ELLDSRVV+ +FSP  P W
Sbjct: 558 SRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHW 597

[Close]

BLAST of MC10g_new0181 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 748.8 bits (1932), Expect = 3.8e-216
Identity = 368/578 (63.67%), Postives = 455/578 (78.72%), Query Frame = 0


Query: 98  KNKATIQFIEDVTANADQVQRCVLTEILSRNASAEYLTRHGLRPNSSPETFKKTIPIISY 157
           K+   ++FIE++T N D VQ+ V+ EILSRN+  EYL R GL+  +  +TFK  +P++ Y
Sbjct: 18  KDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKTKVPVVIY 77

Query: 158 DHIHPDITRIANGDSSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRSLLYSLLMP 217
           D + P+I RIANGD S IL S PI+EFLTSSGTS GERKLMPTI+E+++RR LLYSLLMP
Sbjct: 78  DDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQLLYSLLMP 137

Query: 218 VMSQFVPGLEKGKGMYFLFIKAESRTPGGLVARPVLTSYYKSSHFKDRPYDPYTNYTSPN 277
           VM+ +VPGL+KGK +YFLF+K ES+TPGGL ARPVLTSYYKS  FK RP DPY  YTSPN
Sbjct: 138 VMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPYNVYTSPN 197

Query: 278 EAILCPDSYQSMYAQLLCGLRQRAEVLRVGAVFASGFIRAIRFLEKHWQLLCHDIRTGTL 337
           EAILCPDS QSMY Q+LCGL  R EVLR+GAVFASG +RAI FL+ +W+ L  DI TGTL
Sbjct: 198 EAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDISTGTL 257

Query: 338 NAQITDQAVREAVMSLLHRPEPELAELIEGEC-KKSSWAGIIRRLWPNTKYVDVIVTGTM 397
           +++I+D A++E++  +L +P+ ELA+ I   C + +SW GII ++WPNTKY+DVIVTG M
Sbjct: 258 SSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIVTGAM 317

Query: 398 SQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYFEFLPVQRN 457
           +QYI  L+YYS GLP+ CTMYASSE YFG+NL P+CKPSEV+YT++P+MAYFEFLP    
Sbjct: 318 AQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLP---- 377

Query: 458 NNANPDSTDEHDLVDLTDVKLGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFNFIC 517
              +   T++ +LV+L DV++GKEYELV+TTYAGL RYRVGDIL+V GF N APQF F+ 
Sbjct: 378 --HHEVPTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFKFVR 437

Query: 518 RKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLAEYTSYANTSTIPGHYVLYWEVNQ 577
           RKNV+LSI+SDKTDE ELQ+AV+N+   L      + EYTSYA T TIPGHYV+YWE+  
Sbjct: 438 RKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYWELLV 497

Query: 578 KG-TTPVPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVECGTFDKLMDYAISM 637
           K  T P    V   CCL +EESLNSVYRQ RV+DKSIGPLEI+VV+ GTF++LMDYAIS 
Sbjct: 498 KDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMDYAISR 557

Query: 638 GASINQYKTPRCVKYEPIVELLDSRVVATYFSPKCPKW 674
           GASINQYK PRCV + PI+ELLDSRVV+T+FSP  P W
Sbjct: 558 GASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHW 589

The following BLAST results are available for this feature:

BLAST of MC10g_new0181 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSQ4	4.3e-281	77.04	Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
O81829	1.3e-277	77.04	Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q8LQM5	2.7e-243	69.24	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo...	[more]
Q60EJ6	2.5e-236	67.64	Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo...	[more]
O82333	1.2e-219	64.94	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=...	[more]

BLAST of MC10g_new0181 vs. NCBI nr


Match Name	Identity	Description
XP_022142856.1	100.00	indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia]	[more]
XP_038905526.1	86.66	indole-3-acetic acid-amido synthetase GH3.6-like [Benincasa hispida]	[more]
XP_023520418.1	84.64	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo]	[more]
XP_022942727.1	86.29	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata]	[more]
XP_022985190.1	84.31	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima]	[more]

BLAST of MC10g_new0181 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1CNF7	100.00	indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 ...	[more]
A0A6J1FR29	86.29	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1JAP2	84.31	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A5D3BYD3	86.36	Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3BTN3	86.36	indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103493...	[more]

BLAST of MC10g_new0181 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54510.1	3.1e-282	77.04	Auxin-responsive GH3 family protein	[more]
AT4G27260.1	9.2e-279	77.04	Auxin-responsive GH3 family protein	[more]
AT2G14960.1	8.8e-221	64.94	Auxin-responsive GH3 family protein	[more]
AT4G37390.1	2.5e-220	63.79	Auxin-responsive GH3 family protein	[more]
AT2G23170.1	3.8e-216	63.67	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 84..104
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 59..79
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 58..85
None	No IPR available	PANTHER	PTHR31901:SF70	BNAC03G14570D PROTEIN	coord: 90..681
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 106..650 e-value: 1.0E-201 score: 671.4
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 90..681

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g_new0181.1	MC10g_new0181.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0010279	indole-3-acetic acid amido synthetase activity