MC10g_new0140 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g_new0140
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionRetrovirus-related Pol polyprotein from transposon RE1
LocationMC10: 6342047 .. 6343092 (+)
RNA-Seq ExpressionMC10g_new0140
SyntenyMC10g_new0140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TGATGATATTATCTTGACTGGGCCTTCTCCACAAGAACTTGACTCTGTTAAGGTCATTCTACGATCTTCTTTTCAACTTAAAGATTTGGGGTCTGTTAAGTATTTTTTGGGGTTTGGAGATTTCTCGTTCCCAACATGGTATTTCTCTTTCTCAATGAAAGTATTGCCTTCAAATACTTGAAGATACCGGCTTTCTCGGGGCTCGTCCTGCTTCTTTTCCCATGGACCCGAATCTTAAGCTTGCTAAGTCTGCTGGCACTCTTCTTTCTGACACATAGGCTACTTCTTATCGTCGTTTACTTGGTCGTTTGCTTTATTTACAGATTTCTTGGCCTGATATATCTTTTTGTGTCCACAAACTTAGTCGCAACATCCATGTTCTGGTCATTTGGCAGCACTTTATCACTTGTTACGTTATCTGAAAGGCTTTCCAGGTCAAGAGATCTTGCTGCAAAAGTCCTCTGTTTTCAATCTCTGTGCTTTTGTTGATGCAAATTAGGGTTCTTGTTTGGATACTCGTCGATCGGTCATCGATTTTTGTGTTTTTCTTGGAGACTCTTTGGTTTTCTTGGCGGTCTAAGAAACAGGCTACTGTTTCTCGTTCTTCTGCTGAAGCAGAATATCGTGCCCTTGCTGCTGTCACTAGCGAGTTGGTGTGGATTCGTCAGTTACTTTATGATTTGTTTATCCCTTTTTCTCCTCCAGTTGTGGTTTATTGTGATAATCAAGTTGCTGTAGCCATTGCATCAAATCCCACATTTCATGAGCGCACGAAGCACATTGCCACTTTGTACGAGACAAGATCGCTAATGGTTTCTTGAAGTTATTACCTATCCGATCTAGTTCACAATTGGCCGATATGTTTACTAAAGCCTTGGGATCCTCTGCTTTACGTTCTTTGCTATCCAGGATGAGTGTTTATGATCTTTACATTCCATCTTGAGGGGGAGTATTAGTGTTAGTCATAGTTTAATTAATACTATGGTAGTTAGATAGTTTGTTATGATATAAGCTTACAGATTGAATTGTATTTATACTGACCAAAT

mRNA sequence

ATGCAAATTAGGGTTCTTGTTTGGATACTCGTCGATCGGTCATCGATTTTTGTGTTTTTCTTGGAGACTCTTTGGTTTTCTTGGCGGTCTAAGAAACAGGCTACTGTTTCTCGTTCTTCTGCTGAAGCAGAATATCGTGCCCTTGCTGCTGTCACTAGCGAGTTGGTGTGGATTCGTCAGTTACTTTATGATTTGTTTATCCCTTTTTCTCCTCCAGTTGTGGTTTATTGTGATAATCAAGTTGCTGTAGCCATTGCATCAAATCCCACATTTCATGAGCGCACGAAGCACATTGCCACTTTGTACGAGACAAGATCGCTAATGGTTTCTTGA

Coding sequence (CDS)

ATGCAAATTAGGGTTCTTGTTTGGATACTCGTCGATCGGTCATCGATTTTTGTGTTTTTCTTGGAGACTCTTTGGTTTTCTTGGCGGTCTAAGAAACAGGCTACTGTTTCTCGTTCTTCTGCTGAAGCAGAATATCGTGCCCTTGCTGCTGTCACTAGCGAGTTGGTGTGGATTCGTCAGTTACTTTATGATTTGTTTATCCCTTTTTCTCCTCCAGTTGTGGTTTATTGTGATAATCAAGTTGCTGTAGCCATTGCATCAAATCCCACATTTCATGAGCGCACGAAGCACATTGCCACTTTGTACGAGACAAGATCGCTAATGGTTTCTTGA

Protein sequence

MQIRVLVWILVDRSSIFVFFLETLWFSWRSKKQATVSRSSAEAEYRALAAVTSELVWIRQLLYDLFIPFSPPVVVYCDNQVAVAIASNPTFHERTKHIATLYETRSLMVS
Homology
BLAST of MC10g_new0140 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 1.3e-14
Identity = 44/86 (51.16%), Postives = 54/86 (62.79%), Query Frame = 0

Query: 17   FVFFLETLWFSWRSKKQATVSRSSAEAEYRALAAVTSELVWIRQLLYDLFIPFSPPVVVY 76
            ++ +L     SW SKKQ  V RSS EAEYR++A  +SEL WI  LL +L I  S P V+Y
Sbjct: 1313 YIVYLGHHPISWSSKKQKGVVRSSTEAEYRSVANTSSELQWICSLLTELGIQLSHPPVIY 1372

Query: 77   CDNQVAVAIASNPTFHERTKHIATLY 103
            CDN  A  + +NP FH R KHIA  Y
Sbjct: 1373 CDNVGATYLCANPVFHSRMKHIALDY 1398

BLAST of MC10g_new0140 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 6.5e-14
Identity = 42/86 (48.84%), Postives = 54/86 (62.79%), Query Frame = 0

Query: 17   FVFFLETLWFSWRSKKQATVSRSSAEAEYRALAAVTSELVWIRQLLYDLFIPFSPPVVVY 76
            ++ +L     SW SKKQ  V RSS EAEYR++A  +SE+ WI  LL +L I  + P V+Y
Sbjct: 1330 YIVYLGHHPISWSSKKQKGVVRSSTEAEYRSVANTSSEMQWICSLLTELGIRLTRPPVIY 1389

Query: 77   CDNQVAVAIASNPTFHERTKHIATLY 103
            CDN  A  + +NP FH R KHIA  Y
Sbjct: 1390 CDNVGATYLCANPVFHSRMKHIAIDY 1415

BLAST of MC10g_new0140 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 67.4 bits (163), Expect = 1.2e-10
Identity = 36/95 (37.89%), Postives = 55/95 (57.89%), Query Frame = 0

Query: 11   VDRSSIFVFFLETLWFS---WRSKKQATVSRSSAEAEYRALAAVTSELVWIRQLLYDLFI 70
            +DR S   +  +   F+   W +K+Q +V+ SS EAEY AL     E +W++ LL  + I
Sbjct: 1261 IDRKSTTGYLFKMFDFNLICWNTKRQNSVAASSTEAEYMALFEAVREALWLKFLLTSINI 1320

Query: 71   PFSPPVVVYCDNQVAVAIASNPTFHERTKHIATLY 103
                P+ +Y DNQ  ++IA+NP+ H+R KHI   Y
Sbjct: 1321 KLENPIKIYEDNQGCISIANNPSCHKRAKHIDIKY 1355

BLAST of MC10g_new0140 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 3.7e-09
Identity = 31/76 (40.79%), Postives = 46/76 (60.53%), Query Frame = 0

Query: 27   SWRSKKQATVSRSSAEAEYRALAAVTSELVWIRQLLYDLFIPFSPPVVVYCDNQVAVAIA 86
            SW+SK Q  V+ S+ EAEY A      E++W+++ L +L +      VVYCD+Q A+ ++
Sbjct: 1206 SWQSKLQKCVALSTTEAEYIAATETGKEMIWLKRFLQELGL-HQKEYVVYCDSQSAIDLS 1265

Query: 87   SNPTFHERTKHIATLY 103
             N  +H RTKHI   Y
Sbjct: 1266 KNSMYHARTKHIDVRY 1280

BLAST of MC10g_new0140 vs. NCBI nr
Match: KAA0065481.1 (putative mitochondrial protein [Cucumis melo var. makuwa] >TYK08722.1 putative mitochondrial protein [Cucumis melo var. makuwa])

HSP 1 Score: 112 bits (280), Expect = 5.87e-28
Identity = 56/88 (63.64%), Postives = 68/88 (77.27%), Query Frame = 0

Query: 13  RSSI--FVFFLETLWFSWRSKKQATVSRSSAEAEYRALAAVTSELVWIRQLLYDLFIPFS 72
           R SI  F  FLE    SW++KKQATVSRSSAEAEYRALA+V+SE+ W+  LL +  IP  
Sbjct: 102 RKSITGFCIFLEKSLVSWKTKKQATVSRSSAEAEYRALASVSSEITWLTHLLQNFQIPHQ 161

Query: 73  PPVVVYCDNQVAVAIASNPTFHERTKHI 98
            P ++YCDN+ A++IASNPTFHERTKHI
Sbjct: 162 EPALIYCDNRAAISIASNPTFHERTKHI 189

BLAST of MC10g_new0140 vs. NCBI nr
Match: XP_022141777.1 (uncharacterized protein LOC111012058 [Momordica charantia])

HSP 1 Score: 112 bits (280), Expect = 2.05e-27
Identity = 59/85 (69.41%), Postives = 67/85 (78.82%), Query Frame = 0

Query: 18  VFFLETLWFSWRSKKQATVSRSSAEAEYRALAAVTSELVWIRQLLYDLFIPFSPPVVVYC 77
           VFF ++L  SW+SKKQATVSRSS EAEYRAL AVTSEL+WI+QLL+D  +P S P  VYC
Sbjct: 171 VFFDDSL-ISWKSKKQATVSRSSTEAEYRALVAVTSELIWIQQLLHDFSVPLSSPAFVYC 230

Query: 78  DNQVAVAIASNPTFHERTKHIATLY 102
           DN  AV+IASNPTFHE  KHI   Y
Sbjct: 231 DNHAAVSIASNPTFHE-PKHIEIDY 253

BLAST of MC10g_new0140 vs. NCBI nr
Match: XP_024024810.1 (uncharacterized protein LOC112092560 [Morus notabilis])

HSP 1 Score: 111 bits (277), Expect = 1.01e-26
Identity = 57/89 (64.04%), Postives = 67/89 (75.28%), Query Frame = 0

Query: 12  DRSSI--FVFFLETLWFSWRSKKQATVSRSSAEAEYRALAAVTSELVWIRQLLYDLFIPF 71
            R S+  F  FL     SW++KKQ+TVSRSSA+AEYRALA  TSELVWI+QLL D  IP 
Sbjct: 194 SRKSVTGFCVFLGESLISWKAKKQSTVSRSSAKAEYRALATATSELVWIQQLLRDFAIPT 253

Query: 72  SPPVVVYCDNQVAVAIASNPTFHERTKHI 98
           SPP + +CD+Q A+ IASNP FHERTKHI
Sbjct: 254 SPPTLPFCDSQAAIHIASNPIFHERTKHI 282

BLAST of MC10g_new0140 vs. NCBI nr
Match: XP_038896371.1 (uncharacterized mitochondrial protein AtMg00810-like [Benincasa hispida])

HSP 1 Score: 111 bits (278), Expect = 1.60e-26
Identity = 56/86 (65.12%), Postives = 63/86 (73.26%), Query Frame = 0

Query: 17  FVFFLETLWFSWRSKKQATVSRSSAEAEYRALAAVTSELVWIRQLLYDLFIPFSPPVVVY 76
           F  FL     SW+SKKQATVSRSSAEAEYRALA+VTSEL+WI QLL DL +    P  ++
Sbjct: 256 FCIFLGAFIISWKSKKQATVSRSSAEAEYRALASVTSELMWISQLLKDLQVKILMPTTIF 315

Query: 77  CDNQVAVAIASNPTFHERTKHIATLY 102
           CDNQ  + IASNP FHERTKHI   Y
Sbjct: 316 CDNQAVIVIASNPMFHERTKHIEIDY 341

BLAST of MC10g_new0140 vs. NCBI nr
Match: XP_016196099.1 (uncharacterized protein LOC107637174 [Arachis ipaensis])

HSP 1 Score: 109 bits (272), Expect = 1.71e-26
Identity = 55/82 (67.07%), Postives = 62/82 (75.61%), Query Frame = 0

Query: 17  FVFFLETLWFSWRSKKQATVSRSSAEAEYRALAAVTSELVWIRQLLYDLFIPFSPPVVVY 76
           F FFLE    SWRSKKQ TV+RSSAEAEYRA+A  T E VW+  LL D  IP S P+V+Y
Sbjct: 131 FCFFLEKSLISWRSKKQHTVARSSAEAEYRAMALATCEGVWLSYLLKDFRIPLSRPIVMY 190

Query: 77  CDNQVAVAIASNPTFHERTKHI 98
           CDNQ A+ IA+NP FHERTKHI
Sbjct: 191 CDNQSALHIAANPVFHERTKHI 212

BLAST of MC10g_new0140 vs. ExPASy TrEMBL
Match: A0A5D3CBP3 (Putative mitochondrial protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold76G00270 PE=4 SV=1)

HSP 1 Score: 112 bits (280), Expect = 2.84e-28
Identity = 56/88 (63.64%), Postives = 68/88 (77.27%), Query Frame = 0

Query: 13  RSSI--FVFFLETLWFSWRSKKQATVSRSSAEAEYRALAAVTSELVWIRQLLYDLFIPFS 72
           R SI  F  FLE    SW++KKQATVSRSSAEAEYRALA+V+SE+ W+  LL +  IP  
Sbjct: 102 RKSITGFCIFLEKSLVSWKTKKQATVSRSSAEAEYRALASVSSEITWLTHLLQNFQIPHQ 161

Query: 73  PPVVVYCDNQVAVAIASNPTFHERTKHI 98
            P ++YCDN+ A++IASNPTFHERTKHI
Sbjct: 162 EPALIYCDNRAAISIASNPTFHERTKHI 189

BLAST of MC10g_new0140 vs. ExPASy TrEMBL
Match: A0A6J1CKT9 (uncharacterized protein LOC111012058 OS=Momordica charantia OX=3673 GN=LOC111012058 PE=4 SV=1)

HSP 1 Score: 112 bits (280), Expect = 9.91e-28
Identity = 59/85 (69.41%), Postives = 67/85 (78.82%), Query Frame = 0

Query: 18  VFFLETLWFSWRSKKQATVSRSSAEAEYRALAAVTSELVWIRQLLYDLFIPFSPPVVVYC 77
           VFF ++L  SW+SKKQATVSRSS EAEYRAL AVTSEL+WI+QLL+D  +P S P  VYC
Sbjct: 171 VFFDDSL-ISWKSKKQATVSRSSTEAEYRALVAVTSELIWIQQLLHDFSVPLSSPAFVYC 230

Query: 78  DNQVAVAIASNPTFHERTKHIATLY 102
           DN  AV+IASNPTFHE  KHI   Y
Sbjct: 231 DNHAAVSIASNPTFHE-PKHIEIDY 253

BLAST of MC10g_new0140 vs. ExPASy TrEMBL
Match: A0A5D3BYJ4 (Reverse transcriptase, RNA-dependent DNA polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold298G00740 PE=4 SV=1)

HSP 1 Score: 108 bits (271), Expect = 1.31e-26
Identity = 62/106 (58.49%), Postives = 73/106 (68.87%), Query Frame = 0

Query: 2   QIRVLVWILVD-------RSSI--FVFFLETLWFSWRSKKQATVSRSSAEAEYRALAAVT 61
           Q+R  V++ VD       R SI  F  FL     SW+SKKQAT+S+SSAEA+Y+ALA VT
Sbjct: 119 QVRQKVFVDVDWGSCPDTRRSIIGFCVFLRDSIISWKSKKQATISKSSAEAKYKALALVT 178

Query: 62  SELVWIRQLLYDLFIPFSPPVVVYCDNQVAVAIASNPTFHERTKHI 98
           SELVWI QLL DL +    P  V+CDNQ A+AIASNP FHER KHI
Sbjct: 179 SELVWISQLLNDLHVKILNPTTVFCDNQAAIAIASNPMFHERMKHI 224

BLAST of MC10g_new0140 vs. ExPASy TrEMBL
Match: A0A2K3JYT3 (Copia protein (Fragment) OS=Trifolium pratense OX=57577 GN=L195_g059548 PE=4 SV=1)

HSP 1 Score: 104 bits (260), Expect = 3.66e-26
Identity = 49/72 (68.06%), Postives = 58/72 (80.56%), Query Frame = 0

Query: 27  SWRSKKQATVSRSSAEAEYRALAAVTSELVWIRQLLYDLFIPFSPPVVVYCDNQVAVAIA 86
           SW+SKKQ+TVSRSSAEAEYRA+AAVT EL W+R LL+DL +    P  ++CDNQ A+ IA
Sbjct: 35  SWKSKKQSTVSRSSAEAEYRAMAAVTCELTWLRYLLHDLRVAHPEPAKLFCDNQAALHIA 94

Query: 87  SNPTFHERTKHI 98
            NP FHERTKHI
Sbjct: 95  GNPIFHERTKHI 106

BLAST of MC10g_new0140 vs. ExPASy TrEMBL
Match: A0A7C8YUP8 (Uncharacterized protein OS=Opuntia streptacantha OX=393608 PE=4 SV=1)

HSP 1 Score: 104 bits (260), Expect = 6.03e-26
Identity = 50/82 (60.98%), Postives = 61/82 (74.39%), Query Frame = 0

Query: 17  FVFFLETLWFSWRSKKQATVSRSSAEAEYRALAAVTSELVWIRQLLYDLFIPFSPPVVVY 76
           F  FL T   SW++KKQ TVS+SSAEAEYRA++A TSEL W+  LL DL IP S P+ ++
Sbjct: 43  FCVFLGTSLISWKTKKQKTVSKSSAEAEYRAMSATTSELEWLSHLLQDLLIPLSLPITLF 102

Query: 77  CDNQVAVAIASNPTFHERTKHI 98
           CDN  A  IA+NP FHERTKH+
Sbjct: 103 CDNMAAQHIAANPVFHERTKHL 124

BLAST of MC10g_new0140 vs. TAIR 10
Match: AT4G23160.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 8 )

HSP 1 Score: 93.6 bits (231), Expect = 1.1e-19
Identity = 44/82 (53.66%), Postives = 58/82 (70.73%), Query Frame = 0

Query: 17  FVFFLETLWFSWRSKKQATVSRSSAEAEYRALAAVTSELVWIRQLLYDLFIPFSPPVVVY 76
           +  FL T   SW+SKKQ  VS+SSAEAEYRAL+  T E++W+ Q   +L +P S P +++
Sbjct: 463 YCMFLGTSLISWKSKKQQVVSKSSAEAEYRALSFATDEMMWLAQFFRELQLPLSKPTLLF 522

Query: 77  CDNQVAVAIASNPTFHERTKHI 99
           CDN  A+ IA+N  FHERTKHI
Sbjct: 523 CDNTAAIHIATNAVFHERTKHI 544

BLAST of MC10g_new0140 vs. TAIR 10
Match: ATMG00810.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 9.7e-05
Identity = 23/41 (56.10%), Postives = 26/41 (63.41%), Query Frame = 0

Query: 17  FVFFLETLWFSWRSKKQATVSRSSAEAEYRALAAVTSELVW 58
           F  FL     SW +K+Q TVSRSS E EYRALA   +EL W
Sbjct: 185 FCTFLGCNIISWSAKRQPTVSRSSTETEYRALALTAAELTW 225

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZT941.3e-1451.16Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O... [more]
Q94HW26.5e-1448.84Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O... [more]
P041461.2e-1037.89Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3[more]
P109783.7e-0940.79Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
Match NameE-valueIdentityDescription
KAA0065481.15.87e-2863.64putative mitochondrial protein [Cucumis melo var. makuwa] >TYK08722.1 putative m... [more]
XP_022141777.12.05e-2769.41uncharacterized protein LOC111012058 [Momordica charantia][more]
XP_024024810.11.01e-2664.04uncharacterized protein LOC112092560 [Morus notabilis][more]
XP_038896371.11.60e-2665.12uncharacterized mitochondrial protein AtMg00810-like [Benincasa hispida][more]
XP_016196099.11.71e-2667.07uncharacterized protein LOC107637174 [Arachis ipaensis][more]
Match NameE-valueIdentityDescription
A0A5D3CBP32.84e-2863.64Putative mitochondrial protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A6J1CKT99.91e-2869.41uncharacterized protein LOC111012058 OS=Momordica charantia OX=3673 GN=LOC111012... [more]
A0A5D3BYJ41.31e-2658.49Reverse transcriptase, RNA-dependent DNA polymerase OS=Cucumis melo var. makuwa ... [more]
A0A2K3JYT33.66e-2668.06Copia protein (Fragment) OS=Trifolium pratense OX=57577 GN=L195_g059548 PE=4 SV=... [more]
A0A7C8YUP86.03e-2660.98Uncharacterized protein OS=Opuntia streptacantha OX=393608 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G23160.11.1e-1953.66cysteine-rich RLK (RECEPTOR-like protein kinase) 8 [more]
ATMG00810.19.7e-0556.10DNA/RNA polymerases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR11439:SF283ZINC FINGER, CCHC-TYPE-RELATEDcoord: 17..98
NoneNo IPR availablePANTHERPTHR11439GAG-POL-RELATED RETROTRANSPOSONcoord: 17..98
NoneNo IPR availableCDDcd09272RNase_HI_RT_Ty1coord: 10..102
e-value: 2.87611E-42
score: 133.748
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 15..102

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g_new0140.1MC10g_new0140.1mRNA