MC10g1344 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g1344
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Protease inhibitor protein
Location	MC10: 16966266 .. 16966457 (+)
RNA-Seq Expression	MC10g1344
Synteny	MC10g1344

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGAAAAGTGATTGGCCAGAGCTTGTCTTTACAAAATTTGCTATTGCTGCTGCAATCATCGAAAAGGAGAATCCCAATGTCAAAGCTCACAAGATCTTAGCTGGGAGTCCGAGAATTTTGAATTTCGATTGTACACGAGTTTGGGTTGATGTCAATATTGAAGATGTGGTTGTTGAAATTCCTAGGGTCGGT

mRNA sequence

GGGAAAAGTGATTGGCCAGAGCTTGTCTTTACAAAATTTGCTATTGCTGCTGCAATCATCGAAAAGGAGAATCCCAATGTCAAAGCTCACAAGATCTTAGCTGGGAGTCCGAGAATTTTGAATTTCGATTGTACACGAGTTTGGGTTGATGTCAATATTGAAGATGTGGTTGTTGAAATTCCTAGGGTCGGT

Coding sequence (CDS)

GGGAAAAGTGATTGGCCAGAGCTTGTCTTTACAAAATTTGCTATTGCTGCTGCAATCATCGAAAAGGAGAATCCCAATGTCAAAGCTCACAAGATCTTAGCTGGGAGTCCGAGAATTTTGAATTTCGATTGTACACGAGTTTGGGTTGATGTCAATATTGAAGATGTGGTTGTTGAAATTCCTAGGGTCGGT

Protein sequence

GKSDWPELVFTKFAIAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDVNIEDVVVEIPRVG

Homology

[Close]

BLAST of MC10g1344 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.8e-09
Identity = 31/64 (48.44%), Postives = 41/64 (64.06%), Query Frame = 0


Query: 1  GKSDWPELVFTKFAIAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDVNIEDVVVEI 60
          GKS WP LV    ++A AIIE++NPNVKA  +  G+P   +F C RV + VN   +VV  
Sbjct: 5  GKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSP 64

Query: 61 PRVG 65
          PR+G
Sbjct: 65 PRIG 68

[Close]

BLAST of MC10g1344 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 3.1e-08
Identity = 29/63 (46.03%), Postives = 37/63 (58.73%), Query Frame = 0


Query: 1  GKSDWPELVFTKFAIAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDVNIEDVVVEI 60
          GK+ WPELV     +AAA +E+EN NV A  +  GS    +F C RVWV VN   VV  +
Sbjct: 6  GKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVTSV 65

Query: 61 PRV 64
          P +
Sbjct: 66 PHI 68

[Close]

BLAST of MC10g1344 vs. ExPASy Swiss-Prot
Match: P80211 (Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 9.1e-08
Identity = 32/63 (50.79%), Postives = 38/63 (60.32%), Query Frame = 0


Query: 1  GKSDWPELVFTKFAIAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDVNIEDVVVEI 60
          GK +WPELV      AAAIIE+ENPNV++      S    +F C RVWV V+   VVV  
Sbjct: 6  GKQEWPELVGEYGYKAAAIIERENPNVRSIVKHERSGFTKDFRCDRVWVVVDSTGVVVRT 65

Query: 61 PRV 64
          PRV
Sbjct: 66 PRV 68

[Close]

BLAST of MC10g1344 vs. ExPASy Swiss-Prot
Match: P24076 (Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 2.0e-07
Identity = 28/64 (43.75%), Postives = 37/64 (57.81%), Query Frame = 0


Query: 1  GKSDWPELVFTKFAIAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDVNIEDVVVEI 60
          GK  WP+LV +  A A A+IE+ENP V+A  +  GSP   +F C RV V V    +V   
Sbjct: 5  GKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERGIVARP 64

Query: 61 PRVG 65
          P +G
Sbjct: 65 PAIG 68

[Close]

BLAST of MC10g1344 vs. ExPASy Swiss-Prot
Match: Q6XNP7 (Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2)

HSP 1 Score: 54.7 bits (130), Expect = 4.5e-07
Identity = 32/65 (49.23%), Postives = 41/65 (63.08%), Query Frame = 0


Query: 2  KSDWPELVFTKFAIAAAIIEKENPNVKAHKILAGSP--RILNFDCTRVWVDVNIEDVVVE 61
          K+ WPELV T   IAA II+ EN NVKA  +  G P  + LNF+  RV+VD N   VV +
Sbjct: 8  KNSWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPITQDLNFNRVRVFVDEN--RVVTQ 67

Query: 62 IPRVG 65
          +P +G
Sbjct: 68 VPAIG 70

[Close]

BLAST of MC10g1344 vs. NCBI nr
Match: KGN56897.1 (hypothetical protein Csa_010510 [Cucumis sativus])

HSP 1 Score: 99.4 bits (246), Expect = 1.29e-25
Identity = 45/63 (71.43%), Postives = 58/63 (92.06%), Query Frame = 0


Query: 2  KSDWPELVFTKFAIAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDVNIEDVVVEIP 61
          K++WPELVF K++IAAAIIEKENP+V+A KIL+G+PRILNFD  RVWVD+N+E+VVV+ P
Sbjct: 9  KTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINVEEVVVKKP 68

Query: 62 RVG 64
          +VG
Sbjct: 69 KVG 71

[Close]

BLAST of MC10g1344 vs. NCBI nr
Match: KAA0049288.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17270.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 95.1 bits (235), Expect = 3.50e-23
Identity = 43/62 (69.35%), Postives = 56/62 (90.32%), Query Frame = 0


Query: 2  KSDWPELVFTKFAIAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDVNIEDVVVEIP 61
          K+ WPELVF K++IAAAIIE+ENP+V+A KIL+G+PRILNFD  RVW DVN+EDV+V++P
Sbjct: 10 KTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVEDVIVKMP 69

Query: 62 RV 63
          +V
Sbjct: 70 KV 71

[Close]

BLAST of MC10g1344 vs. NCBI nr
Match: KAA0049287.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17271.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 91.3 bits (225), Expect = 2.11e-22
Identity = 44/63 (69.84%), Postives = 52/63 (82.54%), Query Frame = 0


Query: 2  KSDWPELVFTKFAIAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDVNIEDVVVEIP 61
          K+ WPELVF K++ AAAIIEKENP+VKA KIL+G  RI NFD  RVW DVN+EDVVV+ P
Sbjct: 10 KTQWPELVFVKYSTAAAIIEKENPDVKAVKILSGRFRIQNFDTKRVWCDVNVEDVVVKTP 69

Query: 62 RVG 64
          +VG
Sbjct: 70 KVG 72

[Close]

BLAST of MC10g1344 vs. NCBI nr
Match: KAA0049286.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17272.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 90.5 bits (223), Expect = 4.15e-22
Identity = 44/63 (69.84%), Postives = 52/63 (82.54%), Query Frame = 0


Query: 2  KSDWPELVFTKFAIAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDVNIEDVVVEIP 61
          K+ WPELVF +F+ AA IIEKENP+VKA KILA SPRI NFD +RVWVD N+ED VV++P
Sbjct: 9  KTRWPELVFVEFSTAARIIEKENPDVKAIKILADSPRIENFDPSRVWVDCNVEDKVVKVP 68

Query: 62 RVG 64
           VG
Sbjct: 69 SVG 71

[Close]

BLAST of MC10g1344 vs. NCBI nr
Match: KAE8650337.1 (hypothetical protein Csa_010581 [Cucumis sativus])

HSP 1 Score: 84.0 bits (206), Expect = 3.21e-18
Identity = 41/60 (68.33%), Postives = 47/60 (78.33%), Query Frame = 0


Query: 2  KSDWPELVFTKFAIAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDVNIEDVVVEIP 61
          K+ WPELVF  F  AA IIEKENP+VKA KIL  SPRI NFD +RVWVD NIE+ VV++P
Sbjct: 9  KTRWPELVFVNFCTAARIIEKENPDVKAIKILVDSPRIQNFDISRVWVDCNIEERVVKVP 68

[Close]

BLAST of MC10g1344 vs. ExPASy TrEMBL
Match: A0A0A0L8E6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142910 PE=3 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 6.23e-26
Identity = 45/63 (71.43%), Postives = 58/63 (92.06%), Query Frame = 0


Query: 2  KSDWPELVFTKFAIAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDVNIEDVVVEIP 61
          K++WPELVF K++IAAAIIEKENP+V+A KIL+G+PRILNFD  RVWVD+N+E+VVV+ P
Sbjct: 9  KTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINVEEVVVKKP 68

Query: 62 RVG 64
          +VG
Sbjct: 69 KVG 71

[Close]

BLAST of MC10g1344 vs. ExPASy TrEMBL
Match: A0A5A7U228 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001230 PE=3 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.69e-23
Identity = 43/62 (69.35%), Postives = 56/62 (90.32%), Query Frame = 0


Query: 2  KSDWPELVFTKFAIAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDVNIEDVVVEIP 61
          K+ WPELVF K++IAAAIIE+ENP+V+A KIL+G+PRILNFD  RVW DVN+EDV+V++P
Sbjct: 10 KTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVEDVIVKMP 69

Query: 62 RV 63
          +V
Sbjct: 70 KV 71

[Close]

BLAST of MC10g1344 vs. ExPASy TrEMBL
Match: A0A5A7U4L9 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001240 PE=3 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.02e-22
Identity = 44/63 (69.84%), Postives = 52/63 (82.54%), Query Frame = 0


Query: 2  KSDWPELVFTKFAIAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDVNIEDVVVEIP 61
          K+ WPELVF K++ AAAIIEKENP+VKA KIL+G  RI NFD  RVW DVN+EDVVV+ P
Sbjct: 10 KTQWPELVFVKYSTAAAIIEKENPDVKAVKILSGRFRIQNFDTKRVWCDVNVEDVVVKTP 69

Query: 62 RVG 64
          +VG
Sbjct: 70 KVG 72

[Close]

BLAST of MC10g1344 vs. ExPASy TrEMBL
Match: A0A5A7U4U0 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001250 PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.01e-22
Identity = 44/63 (69.84%), Postives = 52/63 (82.54%), Query Frame = 0


Query: 2  KSDWPELVFTKFAIAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDVNIEDVVVEIP 61
          K+ WPELVF +F+ AA IIEKENP+VKA KILA SPRI NFD +RVWVD N+ED VV++P
Sbjct: 9  KTRWPELVFVEFSTAARIIEKENPDVKAIKILADSPRIENFDPSRVWVDCNVEDKVVKVP 68

Query: 62 RVG 64
           VG
Sbjct: 69 SVG 71

[Close]

BLAST of MC10g1344 vs. ExPASy TrEMBL
Match: A0A0A0L785 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141900 PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 2.35e-21
Identity = 43/63 (68.25%), Postives = 49/63 (77.78%), Query Frame = 0


Query: 2  KSDWPELVFTKFAIAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDVNIEDVVVEIP 61
          K+ WPELVF  F  AA IIEKENP+VKA KIL  SPRI NFD +RVWVD NIE+ VV++P
Sbjct: 9  KTRWPELVFVNFCTAARIIEKENPDVKAIKILVDSPRIQNFDISRVWVDCNIEERVVKVP 68

Query: 62 RVG 64
           VG
Sbjct: 69 SVG 71

[Close]

BLAST of MC10g1344 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 57.8 bits (138), Expect = 3.8e-09
Identity = 30/63 (47.62%), Postives = 38/63 (60.32%), Query Frame = 0


Query: 2  KSDWPELVFTKFAIAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDVNIEDVVVEIP 61
          K+ WPEL  T    AA +IE+ENP V A  IL GSP   +F C RV V V+   +VV+ P
Sbjct: 8  KNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTP 67

Query: 62 RVG 65
          + G
Sbjct: 68 KSG 70

[Close]

BLAST of MC10g1344 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 56.6 bits (135), Expect = 8.4e-09
Identity = 31/64 (48.44%), Postives = 41/64 (64.06%), Query Frame = 0


Query: 1  GKSDWPELVFTKFAIAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDVNIEDVVVEI 60
          GKS WPEL+  K   A  +IE+ENP +KA  IL G+     F C+RV+V VN   +VV+I
Sbjct: 36 GKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQI 95

Query: 61 PRVG 65
          P +G
Sbjct: 96 PIIG 99

[Close]

BLAST of MC10g1344 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 51.2 bits (121), Expect = 3.5e-07
Identity = 27/63 (42.86%), Postives = 34/63 (53.97%), Query Frame = 0


Query: 2  KSDWPELVFTKFAIAAAIIEKENPNVKAHKILAGSPRILNFDCTRVWVDVNIEDVVVEIP 61
          K  WPELV     +A   IE+ENPNV A  +  G  RI NF C RV+V +     V + P
Sbjct: 23 KYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLGSNGQVADAP 82

Query: 62 RVG 65
           +G
Sbjct: 83 MIG 85

The following BLAST results are available for this feature:

BLAST of MC10g1344 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19873	2.8e-09	48.44	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
P82381	3.1e-08	46.03	Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1	[more]
P80211	9.1e-08	50.79	Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1	[more]
P24076	2.0e-07	43.75	Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
Q6XNP7	4.5e-07	49.23	Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2	[more]

BLAST of MC10g1344 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN56897.1	1.29e-25	71.43	hypothetical protein Csa_010510 [Cucumis sativus]	[more]
KAA0049288.1	3.50e-23	69.35	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17270.1 Protease inhib...	[more]
KAA0049287.1	2.11e-22	69.84	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17271.1 Protease inhib...	[more]
KAA0049286.1	4.15e-22	69.84	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17272.1 Protease inhib...	[more]
KAE8650337.1	3.21e-18	68.33	hypothetical protein Csa_010581 [Cucumis sativus]	[more]

BLAST of MC10g1344 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8E6	6.23e-26	71.43	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142910 PE=3 SV=1	[more]
A0A5A7U228	1.69e-23	69.35	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A5A7U4L9	1.02e-22	69.84	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A5A7U4U0	2.01e-22	69.84	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A0A0L785	2.35e-21	68.25	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141900 PE=3 SV=1	[more]

BLAST of MC10g1344 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	3.8e-09	47.62	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	8.4e-09	48.44	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT3G46860.1	3.5e-07	42.86	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 2..64 e-value: 1.0E-17 score: 64.6
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 1..64
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 1..64 e-value: 1.4E-20 score: 75.5
None	No IPR available	PANTHER	PTHR33091:SF62	PROTEASE INHIBITOR PROTEIN	coord: 1..64
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 1..64

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g1344.1	MC10g1344.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity