MC10g1294 (gene) Bitter gourd (Dali-11) v1

BLAST of MC10g1294 vs. ExPASy Swiss-Prot
Match: Q9LQL2 (Protein NRT1/ PTR FAMILY 7.3 OS=Arabidopsis thaliana OX=3702 GN=NPF7.3 PE=1 SV=2)

HSP 1 Score: 929.5 bits (2401), Expect = 2.2e-269
Identity = 459/590 (77.80%), Postives = 513/590 (86.95%), Query Frame = 0

Query: 82  KDQSKLKEEAVAAEEFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLAFFGVGVN 141
           KD  K KE     EE T DGTVD++GRP+IRS SG WVAGI+ILLNQGLATLAFFGVGVN
Sbjct: 9   KDTMKKKE----GEEETRDGTVDYYGRPSIRSNSGQWVAGIVILLNQGLATLAFFGVGVN 68

Query: 142 LVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLV 201
           LVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQ+IFVIGL 
Sbjct: 69  LVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQVIFVIGLS 128

Query: 202 SLSLSSHLFLIRPKGCGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQF 261
           SLSLSS++FLIRP+GCGD  TPCG+HS  EI++FY SIYL ALG GGYQPNIAT GADQF
Sbjct: 129 SLSLSSYMFLIRPRGCGDEVTPCGSHSMMEITMFYFSIYLIALGYGGYQPNIATLGADQF 188

Query: 262 DEEDPKEGYSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASAVAALLLF 321
           DEE PKEGYSK+AFFSYFYLALNLGSLFSNTILG+FEDEGMWALGFW S  SA+  L+LF
Sbjct: 189 DEEHPKEGYSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWALGFWASTGSAIIGLILF 248

Query: 322 LAGTSRYRHFKPTGNPLTRVCQVVVSAAKKWRVRVP-SGGEGLFEDD--GKRSPDNGCRK 381
           L GT RYR+FKPTGNPL+R CQV+V+A KK  V  P  G E +++ D  GK +  N  R+
Sbjct: 249 LVGTPRYRYFKPTGNPLSRFCQVLVAATKKSSVEAPLRGREEMYDGDSEGKNASVNTGRR 308

Query: 382 ILHTHGFKFLDKAAYISSRDLSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCTIIYS 441
           I+HT  FKFLDKAAYI++RDL D++Q   NPWR+CP+TQVEEVKCILRL+PIWLCTIIYS
Sbjct: 309 IVHTDEFKFLDKAAYITARDLDDKKQDSVNPWRLCPVTQVEEVKCILRLMPIWLCTIIYS 368

Query: 442 VVFTQMASLFVEQGAAMKTTISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKS 501
           VVFTQMASLFVEQGAAM T++S F+IPPASMSSFDILSVALFIFLYRRVL+P   + KK+
Sbjct: 369 VVFTQMASLFVEQGAAMNTSVSDFKIPPASMSSFDILSVALFIFLYRRVLEPVANRFKKN 428

Query: 502 SSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVPQYAL 561
            SKG+TEL RMG+GL+IAV+AM++AGIVECYRLKYA   CTHC+GSSSLSIFWQ PQY+L
Sbjct: 429 GSKGITELHRMGIGLVIAVIAMIAAGIVECYRLKYADKSCTHCDGSSSLSIFWQAPQYSL 488

Query: 562 IGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTVDRMP 621
           IGASEVFMYVGQLEFFNAQ PDGLKSFGSALCM S+S+GN+VSSLLVTMV+KIST D MP
Sbjct: 489 IGASEVFMYVGQLEFFNAQTPDGLKSFGSALCMMSMSMGNFVSSLLVTMVVKISTEDHMP 548

Query: 622 GWIPGNLNKGHLDRFYFLLAALTVVDFVIYIACAKWYKSIKLEDKYEQTE 669
           GWIP NLNKGHLDRFYFLLAALT +D V+YIACAKWYK I+LE K E  +
Sbjct: 549 GWIPRNLNKGHLDRFYFLLAALTSIDLVVYIACAKWYKPIQLEGKDEMQD 594

BLAST of MC10g1294 vs. ExPASy Swiss-Prot
Match: Q8GXN2 (Protein NRT1/ PTR FAMILY 7.2 OS=Arabidopsis thaliana OX=3702 GN=NPF7.2 PE=2 SV=2)

HSP 1 Score: 778.9 bits (2010), Expect = 4.8e-224
Identity = 395/583 (67.75%), Postives = 466/583 (79.93%), Query Frame = 0

Query: 95  EEFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLAFFGVGVNLVLFLTRVLQQNN 154
           E  T DG+VD HG PAIR+ +G W+  I+IL+NQGLATLAFFGVGVNLVLFLTRV+ Q+N
Sbjct: 9   EVCTQDGSVDRHGNPAIRANTGKWLTAILILVNQGLATLAFFGVGVNLVLFLTRVMGQDN 68

Query: 155 ADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSLSSHLFLIRP 214
           A+AANNVSKWTGTVYIFSL+GAFLSDSYWGRYKTCAIFQ  FV GL+ LSLS+   L+ P
Sbjct: 69  AEAANNVSKWTGTVYIFSLLGAFLSDSYWGRYKTCAIFQASFVAGLMMLSLSTGALLLEP 128

Query: 215 KGCGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEEDPKEGYSKVA 274
            GCG   +PC  HS  +  LFYLS+YL ALG GGYQPNIATFGADQFD ED  EG+SK+A
Sbjct: 129 SGCGVEDSPCKPHSTFKTVLFYLSVYLIALGYGGYQPNIATFGADQFDAEDSVEGHSKIA 188

Query: 275 FFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASAVAALLLFLAGTSRYRHFKPT 334
           FFSYFYLALNLGSLFSNT+LG+FED+G W LGFW SA SA A L+LFL GT +YRHF P 
Sbjct: 189 FFSYFYLALNLGSLFSNTVLGYFEDQGEWPLGFWASAGSAFAGLVLFLIGTPKYRHFTPR 248

Query: 335 GNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDNGCRKILHTHGFKFLDKAAYI 394
            +P +R CQV+V+A +K ++ V      L++ + + + D   +KILHT GF+FLD+AA +
Sbjct: 249 ESPWSRFCQVLVAATRKAKIDVHHEELNLYDSETQYTGD---KKILHTKGFRFLDRAAIV 308

Query: 395 SSRDLSDQEQ--GVCNPWRICPITQVEEVKCILRLLPIWLCTIIYSVVFTQMASLFVEQG 454
           +  D +++ +     +PWR+C +TQVEEVKC+LRLLPIWLCTI+YSVVFTQMASLFV QG
Sbjct: 309 TPDDEAEKVESGSKYDPWRLCSVTQVEEVKCVLRLLPIWLCTILYSVVFTQMASLFVVQG 368

Query: 455 AAMKTTISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSS-SKGLTELQRMGV 514
           AAMKT I +FRIP +SMSSFDILSVA FIF YRR LDP   +L K+  +KGLTELQRMG+
Sbjct: 369 AAMKTNIKNFRIPASSMSSFDILSVAFFIFAYRRFLDPLFARLNKTERNKGLTELQRMGI 428

Query: 515 GLIIAVMAMVSAGIVECYRLKYAKAD-CTHCEGSSSLSIFWQVPQYALIGASEVFMYVGQ 574
           GL+IA+MAM+SAGIVE +RLK  + +  T    SS+LSIFWQVPQY LIGASEVFMYVGQ
Sbjct: 429 GLVIAIMAMISAGIVEIHRLKNKEPESATSISSSSTLSIFWQVPQYMLIGASEVFMYVGQ 488

Query: 575 LEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTVDRMPGWIPGNLNKGHL 634
           LEFFN+QAP GLKSF SALCM SISLGNYVSSLLV++VMKIST D + GWIP NLNKGHL
Sbjct: 489 LEFFNSQAPTGLKSFASALCMASISLGNYVSSLLVSIVMKISTTDDVHGWIPENLNKGHL 548

Query: 635 DRFYFLLAALTVVDFVIYIACAKWYKSIKLEDKYEQ--TEEPE 672
           +RFYFLLA LT  DFV+Y+ CAKWYK IK E  + +  TEE E
Sbjct: 549 ERFYFLLAGLTAADFVVYLICAKWYKYIKSEASFSESVTEEEE 588

BLAST of MC10g1294 vs. ExPASy Swiss-Prot
Match: Q3E9B5 (Protein NRT1/ PTR FAMILY 7.1 OS=Arabidopsis thaliana OX=3702 GN=NPF7.1 PE=2 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 9.1e-183
Identity = 327/550 (59.45%), Postives = 399/550 (72.55%), Query Frame = 0

Query: 114 KSGTWVAGIIILLNQGLATLAFFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSL 173
           K+G W   II+L+NQGLATLAFFGVGVNLVLFLTRV+ Q NA+AANNVSKWTGTVY+FSL
Sbjct: 59  KNGGWTNAIILLVNQGLATLAFFGVGVNLVLFLTRVMGQGNAEAANNVSKWTGTVYMFSL 118

Query: 174 VGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSLSSHLFLIRPKGCGDLQTPCGTHSKTEIS 233
           VGAFLSDSYWGRY TC IFQ+IFVIG+  LS  S  FLI+P+GCGD    C   S   ++
Sbjct: 119 VGAFLSDSYWGRYLTCTIFQVIFVIGVGLLSFVSWFFLIKPRGCGDGDLECNPPSSLGVA 178

Query: 234 LFYLSIYLTALGNGGYQPNIATFGADQFDEEDPKEGYSKVAFFSYFYLALNLGSLFSNTI 293
           +FYLS+YL A G GG+QP +ATFGADQ D++      SK AFFSYFY ALN+G+LFSNTI
Sbjct: 179 IFYLSVYLVAFGYGGHQPTLATFGADQLDDDK----NSKAAFFSYFYFALNVGALFSNTI 238

Query: 294 LGFFEDEGMWALGFWVSAASAVAALLLFLAGTSRYRHFKPTGNPLTRVCQVVVSAAKKWR 353
           L +FED+G+W  GF VS  SA+ AL+ FLA T +YR+ KP GNPL RV QV V+ A+KW 
Sbjct: 239 LVYFEDKGLWTEGFLVSLGSAIVALVAFLAPTRQYRYVKPCGNPLPRVAQVFVATARKWS 298

Query: 354 VRVPSGGEGLFEDDGKRSPDNGCRKILHTHGFKFLDKAAYISSRDLSDQEQGVCNPWRIC 413
           V  P     L+E +G  S   G RKI H+  F FLD+AA I+  D +       N WR+C
Sbjct: 299 VVRPGDPHELYELEGPESAIKGSRKIFHSTKFLFLDRAAVITENDRNGTRS---NAWRLC 358

Query: 414 PITQVEEVKCILRLLPIWLCTIIYSVVFTQMASLFVEQGAAMKTTISSFRIPPASMSSFD 473
            +TQVEE KC+++LLPIWLCTIIYSV+FTQMASLFVEQG  M   +  F IP ASMS FD
Sbjct: 359 SVTQVEEAKCVMKLLPIWLCTIIYSVIFTQMASLFVEQGDVMNAYVGKFHIPAASMSVFD 418

Query: 474 ILSVALFIFLYRRVLDPFVGKLKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKY 533
           I SV +   +YR ++ P+V           TEL RMG+GLII +MAMV+AG+ E  RLK 
Sbjct: 419 IFSVFVSTGIYRHIIFPYVRP---------TELMRMGIGLIIGIMAMVAAGLTEIQRLKR 478

Query: 534 AKADCTHCEGSSSLSIFWQVPQYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTS 593
                   +  S L+I WQ+PQY L+GASEVFMYVGQLEFFN QAPDGLK+ GS+LCM S
Sbjct: 479 VVPG----QKESELTILWQIPQYVLVGASEVFMYVGQLEFFNGQAPDGLKNLGSSLCMAS 538

Query: 594 ISLGNYVSSLLVTMVMKIS-TVDRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIACA 653
           ++LGNYVSSL+V +VM I+   +  PGWIP NLN+GH+DRFYFL+AAL  +DFV+Y+  A
Sbjct: 539 MALGNYVSSLMVNIVMAITKRGENSPGWIPENLNEGHMDRFYFLIAALAAIDFVVYLIFA 588

Query: 654 KWYKSIKLED 663
           KWY+ I  ++
Sbjct: 599 KWYQPISHDE 588

BLAST of MC10g1294 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 3.7e-136
Identity = 249/561 (44.39%), Postives = 371/561 (66.13%), Query Frame = 0

Query: 97  FTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLAFFGVGVNLVLFLTRVLQQNNAD 156
           +T DGTVD H  PA + K+G W A   IL N+    LA++G+G NLV +L   L Q NA 
Sbjct: 7   YTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNAT 66

Query: 157 AANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSLSSHLFLIRPKG 216
           AANNV+ W+GT YI  L+GAF++D+Y GRY T A F  I+V G+  L+LS+ +  ++P  
Sbjct: 67  AANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGN 126

Query: 217 CGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEEDPKEGYSKVAFF 276
           C      C  +S ++ ++F++++Y+ ALG GG +P +++FGADQFDE D  E   K +FF
Sbjct: 127 CN--ADTCHPNS-SQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFF 186

Query: 277 SYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASAVAALLLFLAGTSRYRHFKPTGN 336
           ++FY ++N+G+L + T+L + +    W  GF V   + V A+  F  G+  YR  +P G+
Sbjct: 187 NWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGS 246

Query: 337 PLTRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDNGCRKILHTHGFKFLDKAAYISS 396
           PLTR+ QV+V+A +K  V+VP     LFE     S   G RK++HT   KF DKAA  S 
Sbjct: 247 PLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQ 306

Query: 397 RDLSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCTIIYSVVFTQMASLFVEQGAAMK 456
            D    + G  NPWR+C +TQVEE+K I+ LLP+W   I+++ V++QM+++FV QG  M 
Sbjct: 307 SD--SIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMD 366

Query: 457 TTI-SSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLTELQRMGVGLII 516
             +  +F IP AS+S FD +SV  +  +Y + + P   K  + + +G T+LQRMG+GL++
Sbjct: 367 QHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTR-NERGFTQLQRMGIGLVV 426

Query: 517 AVMAMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVPQYALIGASEVFMYVGQLEFFN 576
           ++ AM++AG++E  RL Y K    + +    +SIFWQ+PQY LIG +EVF ++GQLEFF 
Sbjct: 427 SIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFY 486

Query: 577 AQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTVDRMPGWIPGNLNKGHLDRFYF 636
            QAPD ++S  SAL +T+++LGNY+S++LVT+VMKI+  +  PGWIP NLN+GHLD F++
Sbjct: 487 DQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFY 546

Query: 637 LLAALTVVDFVIYIACAKWYK 657
           LLA L+ ++F++Y+  +K YK
Sbjct: 547 LLATLSFLNFLVYLWISKRYK 561

BLAST of MC10g1294 vs. ExPASy Swiss-Prot
Match: Q93Z20 (Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 2.1e-131
Identity = 238/559 (42.58%), Postives = 362/559 (64.76%), Query Frame = 0

Query: 100 DGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLAFFGVGVNLVLFLTRVLQQNNADAAN 159
           DG++D +G P  + K+G W A   IL N+    LA++G+  NL+ + T  L ++N  AA+
Sbjct: 38  DGSIDIYGNPPSKKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELHESNVSAAS 97

Query: 160 NVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSLSSHLFLIRPKGC-G 219
           +V  W GT YI  L+GA ++DSYWGRY T A F  I+ IG+  L+LS+ L +++P  C G
Sbjct: 98  DVMIWQGTCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVLKPAACAG 157

Query: 220 DLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEEDPKEGYSKVAFFSY 279
                C   +  + ++F+  +YL ALG GG +P +++FGADQFD+ DP+E   K +FF++
Sbjct: 158 VAAALCSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFNW 217

Query: 280 FYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASAVAALLLFLAGTSRYRHFKPTGNPL 339
           FY ++N+GS  S+T+L + ++   W LGF +       ++  F  GT  YR  KP G+P+
Sbjct: 218 FYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSPI 277

Query: 340 TRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDNGCRKILHTHGFKFLDKAAYISSRD 399
           TRVCQV+V+A +K ++ +P     L+E   K S   G RKI HT G+KFLDKAA IS  +
Sbjct: 278 TRVCQVLVAAYRKLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKAAVIS--E 337

Query: 400 LSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCTIIYSVVFTQMASLFVEQGAAMKTT 459
              +     NPW++C +TQVEEVK ++R+ PIW   I+YSV+++Q+++LFV+QG +M   
Sbjct: 338 YESKSGAFSNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMNRI 397

Query: 460 ISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLTELQRMGVGLIIAVM 519
           I SF IPPAS   FD L V + I +Y R L PFV +      KGLT+LQRMG+GL ++V+
Sbjct: 398 IRSFEIPPASFGVFDTLIVLISIPIYDRFLVPFVRRF-TGIPKGLTDLQRMGIGLFLSVL 457

Query: 520 AMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVPQYALIGASEVFMYVGQLEFFNAQA 579
           ++ +A IVE  RL+ A       +   ++SIFWQ+PQY L+G +EVF ++G++EFF  ++
Sbjct: 458 SIAAAAIVETVRLQLA-------QDFVAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDES 517

Query: 580 PDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTVDRMPGWIPGNLNKGHLDRFYFLLA 639
           PD ++S  SAL + + ++G+Y+SSL++T+V   + +    GW+P +LNKGHLD F++LL 
Sbjct: 518 PDAMRSVCSALALLNTAVGSYLSSLILTLVAYFTALGGKDGWVPDDLNKGHLDYFFWLLV 577

Query: 640 ALTVVDFVIY-IACAKWYK 657
           +L +V+  +Y + C K  K
Sbjct: 578 SLGLVNIPVYALICVKHTK 586

BLAST of MC10g1294 vs. NCBI nr
Match: XP_022155555.1 (protein NRT1/ PTR FAMILY 7.3-like [Momordica charantia])

HSP 1 Score: 1202 bits (3109), Expect = 0.0
Identity = 601/601 (100.00%), Postives = 601/601 (100.00%), Query Frame = 0

Query: 75  MAALKPFKDQSKLKEEAVAAEEFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLA 134
           MAALKPFKDQSKLKEEAVAAEEFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLA
Sbjct: 1   MAALKPFKDQSKLKEEAVAAEEFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLA 60

Query: 135 FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 194
           FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI
Sbjct: 61  FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 120

Query: 195 IFVIGLVSLSLSSHLFLIRPKGCGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIA 254
           IFVIGLVSLSLSSHLFLIRPKGCGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIA
Sbjct: 121 IFVIGLVSLSLSSHLFLIRPKGCGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIA 180

Query: 255 TFGADQFDEEDPKEGYSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASA 314
           TFGADQFDEEDPKEGYSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASA
Sbjct: 181 TFGADQFDEEDPKEGYSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASA 240

Query: 315 VAALLLFLAGTSRYRHFKPTGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDN 374
           VAALLLFLAGTSRYRHFKPTGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDN
Sbjct: 241 VAALLLFLAGTSRYRHFKPTGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDN 300

Query: 375 GCRKILHTHGFKFLDKAAYISSRDLSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCT 434
           GCRKILHTHGFKFLDKAAYISSRDLSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCT
Sbjct: 301 GCRKILHTHGFKFLDKAAYISSRDLSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCT 360

Query: 435 IIYSVVFTQMASLFVEQGAAMKTTISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGK 494
           IIYSVVFTQMASLFVEQGAAMKTTISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGK
Sbjct: 361 IIYSVVFTQMASLFVEQGAAMKTTISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGK 420

Query: 495 LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVP 554
           LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVP
Sbjct: 421 LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVP 480

Query: 555 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV 614
           QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV
Sbjct: 481 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV 540

Query: 615 DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIACAKWYKSIKLEDKYEQTEEPESFK 674
           DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIACAKWYKSIKLEDKYEQTEEPESFK
Sbjct: 541 DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIACAKWYKSIKLEDKYEQTEEPESFK 600

BLAST of MC10g1294 vs. NCBI nr
Match: XP_038906251.1 (protein NRT1/ PTR FAMILY 7.3-like [Benincasa hispida])

HSP 1 Score: 1135 bits (2935), Expect = 0.0
Identity = 567/601 (94.34%), Postives = 583/601 (97.00%), Query Frame = 0

Query: 75  MAALKPFKDQSKLKEEAVAAEEFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLA 134
           MA ++ F+DQSKLKEE  AAE FTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLA
Sbjct: 1   MAVIQSFEDQSKLKEEIAAAEGFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLA 60

Query: 135 FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 194
           FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI
Sbjct: 61  FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 120

Query: 195 IFVIGLVSLSLSSHLFLIRPKGCGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIA 254
           IFVIGLVSLSLSS+LFLIRPKGCGD QTPCG+HSKTEISLFYLSIYLTALGNGGYQPNIA
Sbjct: 121 IFVIGLVSLSLSSYLFLIRPKGCGDEQTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIA 180

Query: 255 TFGADQFDEEDPKEGYSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASA 314
           TFGADQFDEE  KEG+SKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVS  SA
Sbjct: 181 TFGADQFDEEYQKEGHSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSTGSA 240

Query: 315 VAALLLFLAGTSRYRHFKPTGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDN 374
           VAALLLFL GT RYRHFKPTGNPLTRV QVV+SAAKKWRV VPS GEGLF+DDGK+SP+N
Sbjct: 241 VAALLLFLIGTPRYRHFKPTGNPLTRVSQVVISAAKKWRVMVPSDGEGLFDDDGKQSPNN 300

Query: 375 GCRKILHTHGFKFLDKAAYISSRDLSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCT 434
           GCRKILHTHGFKFLDKAAYISSRDLSD+EQGV NPWR+CPITQVEEVKCILRLLPIWLCT
Sbjct: 301 GCRKILHTHGFKFLDKAAYISSRDLSDKEQGVNNPWRLCPITQVEEVKCILRLLPIWLCT 360

Query: 435 IIYSVVFTQMASLFVEQGAAMKTTISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGK 494
           IIYSVVFTQMASLFVEQGAAMKTTIS+F IPPASMSSFDILSVALFIFLYRRVLDPFVGK
Sbjct: 361 IIYSVVFTQMASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGK 420

Query: 495 LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVP 554
           LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYA+ADCTHCEGSSSLSIFWQVP
Sbjct: 421 LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVP 480

Query: 555 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV 614
           QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV
Sbjct: 481 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV 540

Query: 615 DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIACAKWYKSIKLEDKYEQTEEPESFK 674
           DRMPGWIPGNLNKGHLDRFYFLLAALTV+DFVIYIACAKWYKSIKLE+KYEQTEE ESFK
Sbjct: 541 DRMPGWIPGNLNKGHLDRFYFLLAALTVIDFVIYIACAKWYKSIKLEEKYEQTEEQESFK 600

BLAST of MC10g1294 vs. NCBI nr
Match: XP_008448722.1 (PREDICTED: protein NRT1/ PTR FAMILY 7.3-like [Cucumis melo])

HSP 1 Score: 1130 bits (2923), Expect = 0.0
Identity = 567/601 (94.34%), Postives = 581/601 (96.67%), Query Frame = 0

Query: 75  MAALKPFKDQSKLKEEAVAAEEFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLA 134
           MA+L+ F+DQSKLKEE   AE FTLDGTVD+HGRPAIRSKSGTWVAGIIILLNQGLATLA
Sbjct: 1   MASLQSFEDQSKLKEEIATAEGFTLDGTVDFHGRPAIRSKSGTWVAGIIILLNQGLATLA 60

Query: 135 FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 194
           FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI
Sbjct: 61  FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 120

Query: 195 IFVIGLVSLSLSSHLFLIRPKGCGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIA 254
           IFVIGLVSLSLSSHLFLIRPKGCGD QTPCG+HSKTEISLFYLSIYLTALGNGGYQPNIA
Sbjct: 121 IFVIGLVSLSLSSHLFLIRPKGCGDEQTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIA 180

Query: 255 TFGADQFDEEDPKEGYSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASA 314
           TFGADQFDEE  KEG+SKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSA SA
Sbjct: 181 TFGADQFDEEYQKEGHSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSA 240

Query: 315 VAALLLFLAGTSRYRHFKPTGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDN 374
            AALLLFL GT RYRHFKPTGNPLTRV QVVVSAAKKWRV+VPSGGEGLF+DDGK S +N
Sbjct: 241 AAALLLFLIGTPRYRHFKPTGNPLTRVSQVVVSAAKKWRVKVPSGGEGLFDDDGKESSNN 300

Query: 375 GCRKILHTHGFKFLDKAAYISSRDLSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCT 434
           GCRKILHTHGFKFLDKAAYISSRDLSD+EQGV NPWR+CPITQVEEVKCILRLLPIWLCT
Sbjct: 301 GCRKILHTHGFKFLDKAAYISSRDLSDKEQGVNNPWRLCPITQVEEVKCILRLLPIWLCT 360

Query: 435 IIYSVVFTQMASLFVEQGAAMKTTISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGK 494
           IIYSVVFTQMASLFVEQGAAMKTTIS+F IPPASMSSFDILSVALFIFLYRRVLDPFVGK
Sbjct: 361 IIYSVVFTQMASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGK 420

Query: 495 LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVP 554
           LKKSSS GLTELQRMGVGLIIAVMAMVSAGIVECYRLKYA+ADCTHCEGSSSLSIFWQVP
Sbjct: 421 LKKSSSTGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVP 480

Query: 555 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV 614
           QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV
Sbjct: 481 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV 540

Query: 615 DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIACAKWYKSIKLEDKYEQTEEPESFK 674
           DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYI CAKWYKSIKLE+KYEQTEE ESFK
Sbjct: 541 DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIVCAKWYKSIKLEEKYEQTEEQESFK 600

BLAST of MC10g1294 vs. NCBI nr
Match: XP_004150179.2 (protein NRT1/ PTR FAMILY 7.3 isoform X2 [Cucumis sativus] >KGN55790.1 hypothetical protein Csa_010935 [Cucumis sativus])

HSP 1 Score: 1122 bits (2902), Expect = 0.0
Identity = 561/601 (93.34%), Postives = 579/601 (96.34%), Query Frame = 0

Query: 75  MAALKPFKDQSKLKEEAVAAEEFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLA 134
           MA+L+ F+DQSKLKEE   AE FTLDGTVD+HGRPAIRSKSGTWVAGIIILLNQGLATLA
Sbjct: 1   MASLQSFEDQSKLKEEIATAEGFTLDGTVDFHGRPAIRSKSGTWVAGIIILLNQGLATLA 60

Query: 135 FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 194
           FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI
Sbjct: 61  FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 120

Query: 195 IFVIGLVSLSLSSHLFLIRPKGCGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIA 254
           IFVIGLVSLSLSSHLFLIRPKGCGD QTPCG+HSKTEISLFYLSIYLTALGNGGYQPNIA
Sbjct: 121 IFVIGLVSLSLSSHLFLIRPKGCGDEQTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIA 180

Query: 255 TFGADQFDEEDPKEGYSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASA 314
           TFGADQFDEE  KEG+SKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVS  SA
Sbjct: 181 TFGADQFDEEYQKEGHSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSTGSA 240

Query: 315 VAALLLFLAGTSRYRHFKPTGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDN 374
            AALLLFL GT RYR+FKPTGNPL RV QVVVSAAKKWR++VPSGGEGLF+DDGK S +N
Sbjct: 241 AAALLLFLIGTPRYRYFKPTGNPLMRVSQVVVSAAKKWRIKVPSGGEGLFDDDGKESSNN 300

Query: 375 GCRKILHTHGFKFLDKAAYISSRDLSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCT 434
           GCRKILHTHGFKFLDKAAYISSRDLSD+EQGV NPWR+CPITQVEEVKCILRLLPIWLCT
Sbjct: 301 GCRKILHTHGFKFLDKAAYISSRDLSDKEQGVNNPWRLCPITQVEEVKCILRLLPIWLCT 360

Query: 435 IIYSVVFTQMASLFVEQGAAMKTTISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGK 494
           IIYSVVFTQMASLFVEQGAAMKTT+S+F IPPASMSSFDILSVALFIFLYRRVLDPFVGK
Sbjct: 361 IIYSVVFTQMASLFVEQGAAMKTTVSNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGK 420

Query: 495 LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVP 554
           LKKSSS GLTELQRMGVGLIIAVMAMVSAGIVECYRLKYA+ADCTHCEGSSSLSIFWQVP
Sbjct: 421 LKKSSSTGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVP 480

Query: 555 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV 614
           QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV
Sbjct: 481 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV 540

Query: 615 DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIACAKWYKSIKLEDKYEQTEEPESFK 674
           DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYI CAKWYKSIKLE+KYEQTEE E+FK
Sbjct: 541 DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIVCAKWYKSIKLEEKYEQTEEQENFK 600

BLAST of MC10g1294 vs. NCBI nr
Match: XP_022923413.1 (protein NRT1/ PTR FAMILY 7.3-like [Cucurbita moschata])

HSP 1 Score: 1113 bits (2880), Expect = 0.0
Identity = 553/601 (92.01%), Postives = 578/601 (96.17%), Query Frame = 0

Query: 75  MAALKPFKDQSKLKEEAVAAEEFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLA 134
           MA+L  F+DQ KLKEE    EEFTLDGTVD+HGRPA+RSKSG+WVAGIIILLNQGLATLA
Sbjct: 1   MASLPSFEDQGKLKEEIATVEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLA 60

Query: 135 FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 194
           FFGVGVNLVLFLTRVLQQ+N DAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI
Sbjct: 61  FFGVGVNLVLFLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 120

Query: 195 IFVIGLVSLSLSSHLFLIRPKGCGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIA 254
           IFVIGLVSLSLSS+LFLIRPKGCGD  TPCG+HSKTEISLFYLSIYLTALGNGGYQPNIA
Sbjct: 121 IFVIGLVSLSLSSYLFLIRPKGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIA 180

Query: 255 TFGADQFDEEDPKEGYSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASA 314
           TFGADQFDEED KEG+ KVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSA SA
Sbjct: 181 TFGADQFDEEDQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSA 240

Query: 315 VAALLLFLAGTSRYRHFKPTGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDN 374
           +AALLLFLAGT RYRHFKP+GNPLTRVCQVVVSAAKKWRVRVPSGGEGLFED+ K++P+N
Sbjct: 241 IAALLLFLAGTPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNN 300

Query: 375 GCRKILHTHGFKFLDKAAYISSRDLSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCT 434
           GCRKILHT+GFKFLDKAAYISSRD SD+EQG+ NPWR+CPITQVEEVKCIL+L+PIWLCT
Sbjct: 301 GCRKILHTNGFKFLDKAAYISSRDSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCT 360

Query: 435 IIYSVVFTQMASLFVEQGAAMKTTISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGK 494
           IIYSVVFTQMASLFVEQGAAMKTTIS+F IPPASMSSFDILSVALFIFLYRRVLDPFVGK
Sbjct: 361 IIYSVVFTQMASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGK 420

Query: 495 LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVP 554
           LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYA+ADCTHCEGSSSLSIFWQVP
Sbjct: 421 LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVP 480

Query: 555 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV 614
           QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKIST 
Sbjct: 481 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTE 540

Query: 615 DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIACAKWYKSIKLEDKYEQTEEPESFK 674
           D MPGWIPGNLNKGHLDRFYFLLAALTV+DFVIY+ACAKWYK IKLEDKYEQ EE ESFK
Sbjct: 541 DDMPGWIPGNLNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK 600

BLAST of MC10g1294 vs. ExPASy TrEMBL
Match: A0A6J1DMR4 (protein NRT1/ PTR FAMILY 7.3-like OS=Momordica charantia OX=3673 GN=LOC111022668 PE=3 SV=1)

HSP 1 Score: 1202 bits (3109), Expect = 0.0
Identity = 601/601 (100.00%), Postives = 601/601 (100.00%), Query Frame = 0

Query: 75  MAALKPFKDQSKLKEEAVAAEEFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLA 134
           MAALKPFKDQSKLKEEAVAAEEFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLA
Sbjct: 1   MAALKPFKDQSKLKEEAVAAEEFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLA 60

Query: 135 FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 194
           FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI
Sbjct: 61  FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 120

Query: 195 IFVIGLVSLSLSSHLFLIRPKGCGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIA 254
           IFVIGLVSLSLSSHLFLIRPKGCGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIA
Sbjct: 121 IFVIGLVSLSLSSHLFLIRPKGCGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIA 180

Query: 255 TFGADQFDEEDPKEGYSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASA 314
           TFGADQFDEEDPKEGYSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASA
Sbjct: 181 TFGADQFDEEDPKEGYSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASA 240

Query: 315 VAALLLFLAGTSRYRHFKPTGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDN 374
           VAALLLFLAGTSRYRHFKPTGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDN
Sbjct: 241 VAALLLFLAGTSRYRHFKPTGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDN 300

Query: 375 GCRKILHTHGFKFLDKAAYISSRDLSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCT 434
           GCRKILHTHGFKFLDKAAYISSRDLSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCT
Sbjct: 301 GCRKILHTHGFKFLDKAAYISSRDLSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCT 360

Query: 435 IIYSVVFTQMASLFVEQGAAMKTTISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGK 494
           IIYSVVFTQMASLFVEQGAAMKTTISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGK
Sbjct: 361 IIYSVVFTQMASLFVEQGAAMKTTISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGK 420

Query: 495 LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVP 554
           LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVP
Sbjct: 421 LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVP 480

Query: 555 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV 614
           QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV
Sbjct: 481 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV 540

Query: 615 DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIACAKWYKSIKLEDKYEQTEEPESFK 674
           DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIACAKWYKSIKLEDKYEQTEEPESFK
Sbjct: 541 DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIACAKWYKSIKLEDKYEQTEEPESFK 600

BLAST of MC10g1294 vs. ExPASy TrEMBL
Match: A0A1S3BKC8 (protein NRT1/ PTR FAMILY 7.3-like OS=Cucumis melo OX=3656 GN=LOC103490806 PE=3 SV=1)

HSP 1 Score: 1130 bits (2923), Expect = 0.0
Identity = 567/601 (94.34%), Postives = 581/601 (96.67%), Query Frame = 0

Query: 75  MAALKPFKDQSKLKEEAVAAEEFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLA 134
           MA+L+ F+DQSKLKEE   AE FTLDGTVD+HGRPAIRSKSGTWVAGIIILLNQGLATLA
Sbjct: 1   MASLQSFEDQSKLKEEIATAEGFTLDGTVDFHGRPAIRSKSGTWVAGIIILLNQGLATLA 60

Query: 135 FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 194
           FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI
Sbjct: 61  FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 120

Query: 195 IFVIGLVSLSLSSHLFLIRPKGCGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIA 254
           IFVIGLVSLSLSSHLFLIRPKGCGD QTPCG+HSKTEISLFYLSIYLTALGNGGYQPNIA
Sbjct: 121 IFVIGLVSLSLSSHLFLIRPKGCGDEQTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIA 180

Query: 255 TFGADQFDEEDPKEGYSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASA 314
           TFGADQFDEE  KEG+SKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSA SA
Sbjct: 181 TFGADQFDEEYQKEGHSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSA 240

Query: 315 VAALLLFLAGTSRYRHFKPTGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDN 374
            AALLLFL GT RYRHFKPTGNPLTRV QVVVSAAKKWRV+VPSGGEGLF+DDGK S +N
Sbjct: 241 AAALLLFLIGTPRYRHFKPTGNPLTRVSQVVVSAAKKWRVKVPSGGEGLFDDDGKESSNN 300

Query: 375 GCRKILHTHGFKFLDKAAYISSRDLSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCT 434
           GCRKILHTHGFKFLDKAAYISSRDLSD+EQGV NPWR+CPITQVEEVKCILRLLPIWLCT
Sbjct: 301 GCRKILHTHGFKFLDKAAYISSRDLSDKEQGVNNPWRLCPITQVEEVKCILRLLPIWLCT 360

Query: 435 IIYSVVFTQMASLFVEQGAAMKTTISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGK 494
           IIYSVVFTQMASLFVEQGAAMKTTIS+F IPPASMSSFDILSVALFIFLYRRVLDPFVGK
Sbjct: 361 IIYSVVFTQMASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGK 420

Query: 495 LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVP 554
           LKKSSS GLTELQRMGVGLIIAVMAMVSAGIVECYRLKYA+ADCTHCEGSSSLSIFWQVP
Sbjct: 421 LKKSSSTGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVP 480

Query: 555 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV 614
           QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV
Sbjct: 481 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV 540

Query: 615 DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIACAKWYKSIKLEDKYEQTEEPESFK 674
           DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYI CAKWYKSIKLE+KYEQTEE ESFK
Sbjct: 541 DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIVCAKWYKSIKLEEKYEQTEEQESFK 600

BLAST of MC10g1294 vs. ExPASy TrEMBL
Match: A0A0A0L1B9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G015860 PE=3 SV=1)

HSP 1 Score: 1122 bits (2902), Expect = 0.0
Identity = 561/601 (93.34%), Postives = 579/601 (96.34%), Query Frame = 0

Query: 75  MAALKPFKDQSKLKEEAVAAEEFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLA 134
           MA+L+ F+DQSKLKEE   AE FTLDGTVD+HGRPAIRSKSGTWVAGIIILLNQGLATLA
Sbjct: 1   MASLQSFEDQSKLKEEIATAEGFTLDGTVDFHGRPAIRSKSGTWVAGIIILLNQGLATLA 60

Query: 135 FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 194
           FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI
Sbjct: 61  FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 120

Query: 195 IFVIGLVSLSLSSHLFLIRPKGCGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIA 254
           IFVIGLVSLSLSSHLFLIRPKGCGD QTPCG+HSKTEISLFYLSIYLTALGNGGYQPNIA
Sbjct: 121 IFVIGLVSLSLSSHLFLIRPKGCGDEQTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIA 180

Query: 255 TFGADQFDEEDPKEGYSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASA 314
           TFGADQFDEE  KEG+SKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVS  SA
Sbjct: 181 TFGADQFDEEYQKEGHSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSTGSA 240

Query: 315 VAALLLFLAGTSRYRHFKPTGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDN 374
            AALLLFL GT RYR+FKPTGNPL RV QVVVSAAKKWR++VPSGGEGLF+DDGK S +N
Sbjct: 241 AAALLLFLIGTPRYRYFKPTGNPLMRVSQVVVSAAKKWRIKVPSGGEGLFDDDGKESSNN 300

Query: 375 GCRKILHTHGFKFLDKAAYISSRDLSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCT 434
           GCRKILHTHGFKFLDKAAYISSRDLSD+EQGV NPWR+CPITQVEEVKCILRLLPIWLCT
Sbjct: 301 GCRKILHTHGFKFLDKAAYISSRDLSDKEQGVNNPWRLCPITQVEEVKCILRLLPIWLCT 360

Query: 435 IIYSVVFTQMASLFVEQGAAMKTTISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGK 494
           IIYSVVFTQMASLFVEQGAAMKTT+S+F IPPASMSSFDILSVALFIFLYRRVLDPFVGK
Sbjct: 361 IIYSVVFTQMASLFVEQGAAMKTTVSNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGK 420

Query: 495 LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVP 554
           LKKSSS GLTELQRMGVGLIIAVMAMVSAGIVECYRLKYA+ADCTHCEGSSSLSIFWQVP
Sbjct: 421 LKKSSSTGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVP 480

Query: 555 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV 614
           QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV
Sbjct: 481 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV 540

Query: 615 DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIACAKWYKSIKLEDKYEQTEEPESFK 674
           DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYI CAKWYKSIKLE+KYEQTEE E+FK
Sbjct: 541 DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIVCAKWYKSIKLEEKYEQTEEQENFK 600

BLAST of MC10g1294 vs. ExPASy TrEMBL
Match: A0A6J1E9L0 (protein NRT1/ PTR FAMILY 7.3-like OS=Cucurbita moschata OX=3662 GN=LOC111431116 PE=3 SV=1)

HSP 1 Score: 1113 bits (2880), Expect = 0.0
Identity = 553/601 (92.01%), Postives = 578/601 (96.17%), Query Frame = 0

Query: 75  MAALKPFKDQSKLKEEAVAAEEFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLA 134
           MA+L  F+DQ KLKEE    EEFTLDGTVD+HGRPA+RSKSG+WVAGIIILLNQGLATLA
Sbjct: 1   MASLPSFEDQGKLKEEIATVEEFTLDGTVDYHGRPAVRSKSGSWVAGIIILLNQGLATLA 60

Query: 135 FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 194
           FFGVGVNLVLFLTRVLQQ+N DAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI
Sbjct: 61  FFGVGVNLVLFLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 120

Query: 195 IFVIGLVSLSLSSHLFLIRPKGCGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIA 254
           IFVIGLVSLSLSS+LFLIRPKGCGD  TPCG+HSKTEISLFYLSIYLTALGNGGYQPNIA
Sbjct: 121 IFVIGLVSLSLSSYLFLIRPKGCGDEHTPCGSHSKTEISLFYLSIYLTALGNGGYQPNIA 180

Query: 255 TFGADQFDEEDPKEGYSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASA 314
           TFGADQFDEED KEG+ KVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSA SA
Sbjct: 181 TFGADQFDEEDQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSA 240

Query: 315 VAALLLFLAGTSRYRHFKPTGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDN 374
           +AALLLFLAGT RYRHFKP+GNPLTRVCQVVVSAAKKWRVRVPSGGEGLFED+ K++P+N
Sbjct: 241 IAALLLFLAGTPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNN 300

Query: 375 GCRKILHTHGFKFLDKAAYISSRDLSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCT 434
           GCRKILHT+GFKFLDKAAYISSRD SD+EQG+ NPWR+CPITQVEEVKCIL+L+PIWLCT
Sbjct: 301 GCRKILHTNGFKFLDKAAYISSRDSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCT 360

Query: 435 IIYSVVFTQMASLFVEQGAAMKTTISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGK 494
           IIYSVVFTQMASLFVEQGAAMKTTIS+F IPPASMSSFDILSVALFIFLYRRVLDPFVGK
Sbjct: 361 IIYSVVFTQMASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGK 420

Query: 495 LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVP 554
           LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYA+ADCTHCEGSSSLSIFWQVP
Sbjct: 421 LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVP 480

Query: 555 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV 614
           QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKIST 
Sbjct: 481 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTE 540

Query: 615 DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIACAKWYKSIKLEDKYEQTEEPESFK 674
           D MPGWIPGNLNKGHLDRFYFLLAALTV+DFVIY+ACAKWYK IKLEDKYEQ EE ESFK
Sbjct: 541 DDMPGWIPGNLNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK 600

BLAST of MC10g1294 vs. ExPASy TrEMBL
Match: A0A6J1L3U9 (protein NRT1/ PTR FAMILY 7.3-like OS=Cucurbita maxima OX=3661 GN=LOC111500269 PE=3 SV=1)

HSP 1 Score: 1107 bits (2864), Expect = 0.0
Identity = 551/601 (91.68%), Postives = 577/601 (96.01%), Query Frame = 0

Query: 75  MAALKPFKDQSKLKEEAVAAEEFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLA 134
           MA+L  F+DQ KLKEE  A EEFTLDGTV++HG PA+RSKSG+WVAGIIILLNQGLATLA
Sbjct: 1   MASLPSFEDQGKLKEEIAAVEEFTLDGTVNYHGHPAVRSKSGSWVAGIIILLNQGLATLA 60

Query: 135 FFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 194
           FFGVGVNLVLFLTRVLQQ+N DAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI
Sbjct: 61  FFGVGVNLVLFLTRVLQQSNVDAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQI 120

Query: 195 IFVIGLVSLSLSSHLFLIRPKGCGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIA 254
           IFVIGLVSLSLSS+LFLIRPKGCGD  T CG+HSKTEISLFYLSIYLTALGNGGYQPNIA
Sbjct: 121 IFVIGLVSLSLSSYLFLIRPKGCGDEHTLCGSHSKTEISLFYLSIYLTALGNGGYQPNIA 180

Query: 255 TFGADQFDEEDPKEGYSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASA 314
           TFGADQFDEED KEG+ KVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSA SA
Sbjct: 181 TFGADQFDEEDQKEGHYKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAGSA 240

Query: 315 VAALLLFLAGTSRYRHFKPTGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDN 374
           +AALLLFLAGT RYRHFKP+GNPLTRVCQVVVSAAKKWRVRVPSGGEGLFED+ K++P+N
Sbjct: 241 IAALLLFLAGTPRYRHFKPSGNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDNEKQNPNN 300

Query: 375 GCRKILHTHGFKFLDKAAYISSRDLSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCT 434
           GCRKILHT+GFKFLDKAAYISSRD SD+EQG+ NPWR+CPITQVEEVKCIL+L+PIWLCT
Sbjct: 301 GCRKILHTNGFKFLDKAAYISSRDSSDKEQGMINPWRLCPITQVEEVKCILKLMPIWLCT 360

Query: 435 IIYSVVFTQMASLFVEQGAAMKTTISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGK 494
           IIYSVVFTQMASLFVEQGAAMKTTIS+F IPPASMSSFDILSVALFIFLYRRVLDPFVGK
Sbjct: 361 IIYSVVFTQMASLFVEQGAAMKTTISNFHIPPASMSSFDILSVALFIFLYRRVLDPFVGK 420

Query: 495 LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVP 554
           LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYA+ADCTHCEGSSSLSIFWQVP
Sbjct: 421 LKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAQADCTHCEGSSSLSIFWQVP 480

Query: 555 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTV 614
           QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKIST 
Sbjct: 481 QYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTE 540

Query: 615 DRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIACAKWYKSIKLEDKYEQTEEPESFK 674
           D MPGWIPGNLNKGHLDRFYFLLAALTV+DFVIY+ACAKWYK IKLEDKYEQ EE ESFK
Sbjct: 541 DDMPGWIPGNLNKGHLDRFYFLLAALTVIDFVIYVACAKWYKCIKLEDKYEQNEELESFK 600

BLAST of MC10g1294 vs. TAIR 10
Match: AT1G32450.1 (nitrate transporter 1.5 )

HSP 1 Score: 929.5 bits (2401), Expect = 1.6e-270
Identity = 459/590 (77.80%), Postives = 513/590 (86.95%), Query Frame = 0

Query: 82  KDQSKLKEEAVAAEEFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLAFFGVGVN 141
           KD  K KE     EE T DGTVD++GRP+IRS SG WVAGI+ILLNQGLATLAFFGVGVN
Sbjct: 9   KDTMKKKE----GEEETRDGTVDYYGRPSIRSNSGQWVAGIVILLNQGLATLAFFGVGVN 68

Query: 142 LVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLV 201
           LVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQ+IFVIGL 
Sbjct: 69  LVLFLTRVLQQNNADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQVIFVIGLS 128

Query: 202 SLSLSSHLFLIRPKGCGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQF 261
           SLSLSS++FLIRP+GCGD  TPCG+HS  EI++FY SIYL ALG GGYQPNIAT GADQF
Sbjct: 129 SLSLSSYMFLIRPRGCGDEVTPCGSHSMMEITMFYFSIYLIALGYGGYQPNIATLGADQF 188

Query: 262 DEEDPKEGYSKVAFFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASAVAALLLF 321
           DEE PKEGYSK+AFFSYFYLALNLGSLFSNTILG+FEDEGMWALGFW S  SA+  L+LF
Sbjct: 189 DEEHPKEGYSKIAFFSYFYLALNLGSLFSNTILGYFEDEGMWALGFWASTGSAIIGLILF 248

Query: 322 LAGTSRYRHFKPTGNPLTRVCQVVVSAAKKWRVRVP-SGGEGLFEDD--GKRSPDNGCRK 381
           L GT RYR+FKPTGNPL+R CQV+V+A KK  V  P  G E +++ D  GK +  N  R+
Sbjct: 249 LVGTPRYRYFKPTGNPLSRFCQVLVAATKKSSVEAPLRGREEMYDGDSEGKNASVNTGRR 308

Query: 382 ILHTHGFKFLDKAAYISSRDLSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCTIIYS 441
           I+HT  FKFLDKAAYI++RDL D++Q   NPWR+CP+TQVEEVKCILRL+PIWLCTIIYS
Sbjct: 309 IVHTDEFKFLDKAAYITARDLDDKKQDSVNPWRLCPVTQVEEVKCILRLMPIWLCTIIYS 368

Query: 442 VVFTQMASLFVEQGAAMKTTISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKS 501
           VVFTQMASLFVEQGAAM T++S F+IPPASMSSFDILSVALFIFLYRRVL+P   + KK+
Sbjct: 369 VVFTQMASLFVEQGAAMNTSVSDFKIPPASMSSFDILSVALFIFLYRRVLEPVANRFKKN 428

Query: 502 SSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVPQYAL 561
            SKG+TEL RMG+GL+IAV+AM++AGIVECYRLKYA   CTHC+GSSSLSIFWQ PQY+L
Sbjct: 429 GSKGITELHRMGIGLVIAVIAMIAAGIVECYRLKYADKSCTHCDGSSSLSIFWQAPQYSL 488

Query: 562 IGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTVDRMP 621
           IGASEVFMYVGQLEFFNAQ PDGLKSFGSALCM S+S+GN+VSSLLVTMV+KIST D MP
Sbjct: 489 IGASEVFMYVGQLEFFNAQTPDGLKSFGSALCMMSMSMGNFVSSLLVTMVVKISTEDHMP 548

Query: 622 GWIPGNLNKGHLDRFYFLLAALTVVDFVIYIACAKWYKSIKLEDKYEQTE 669
           GWIP NLNKGHLDRFYFLLAALT +D V+YIACAKWYK I+LE K E  +
Sbjct: 549 GWIPRNLNKGHLDRFYFLLAALTSIDLVVYIACAKWYKPIQLEGKDEMQD 594

BLAST of MC10g1294 vs. TAIR 10
Match: AT4G21680.1 (NITRATE TRANSPORTER 1.8 )

HSP 1 Score: 778.9 bits (2010), Expect = 3.4e-225
Identity = 395/583 (67.75%), Postives = 466/583 (79.93%), Query Frame = 0

Query: 95  EEFTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLAFFGVGVNLVLFLTRVLQQNN 154
           E  T DG+VD HG PAIR+ +G W+  I+IL+NQGLATLAFFGVGVNLVLFLTRV+ Q+N
Sbjct: 9   EVCTQDGSVDRHGNPAIRANTGKWLTAILILVNQGLATLAFFGVGVNLVLFLTRVMGQDN 68

Query: 155 ADAANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSLSSHLFLIRP 214
           A+AANNVSKWTGTVYIFSL+GAFLSDSYWGRYKTCAIFQ  FV GL+ LSLS+   L+ P
Sbjct: 69  AEAANNVSKWTGTVYIFSLLGAFLSDSYWGRYKTCAIFQASFVAGLMMLSLSTGALLLEP 128

Query: 215 KGCGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEEDPKEGYSKVA 274
            GCG   +PC  HS  +  LFYLS+YL ALG GGYQPNIATFGADQFD ED  EG+SK+A
Sbjct: 129 SGCGVEDSPCKPHSTFKTVLFYLSVYLIALGYGGYQPNIATFGADQFDAEDSVEGHSKIA 188

Query: 275 FFSYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASAVAALLLFLAGTSRYRHFKPT 334
           FFSYFYLALNLGSLFSNT+LG+FED+G W LGFW SA SA A L+LFL GT +YRHF P 
Sbjct: 189 FFSYFYLALNLGSLFSNTVLGYFEDQGEWPLGFWASAGSAFAGLVLFLIGTPKYRHFTPR 248

Query: 335 GNPLTRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDNGCRKILHTHGFKFLDKAAYI 394
            +P +R CQV+V+A +K ++ V      L++ + + + D   +KILHT GF+FLD+AA +
Sbjct: 249 ESPWSRFCQVLVAATRKAKIDVHHEELNLYDSETQYTGD---KKILHTKGFRFLDRAAIV 308

Query: 395 SSRDLSDQEQ--GVCNPWRICPITQVEEVKCILRLLPIWLCTIIYSVVFTQMASLFVEQG 454
           +  D +++ +     +PWR+C +TQVEEVKC+LRLLPIWLCTI+YSVVFTQMASLFV QG
Sbjct: 309 TPDDEAEKVESGSKYDPWRLCSVTQVEEVKCVLRLLPIWLCTILYSVVFTQMASLFVVQG 368

Query: 455 AAMKTTISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSS-SKGLTELQRMGV 514
           AAMKT I +FRIP +SMSSFDILSVA FIF YRR LDP   +L K+  +KGLTELQRMG+
Sbjct: 369 AAMKTNIKNFRIPASSMSSFDILSVAFFIFAYRRFLDPLFARLNKTERNKGLTELQRMGI 428

Query: 515 GLIIAVMAMVSAGIVECYRLKYAKAD-CTHCEGSSSLSIFWQVPQYALIGASEVFMYVGQ 574
           GL+IA+MAM+SAGIVE +RLK  + +  T    SS+LSIFWQVPQY LIGASEVFMYVGQ
Sbjct: 429 GLVIAIMAMISAGIVEIHRLKNKEPESATSISSSSTLSIFWQVPQYMLIGASEVFMYVGQ 488

Query: 575 LEFFNAQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTVDRMPGWIPGNLNKGHL 634
           LEFFN+QAP GLKSF SALCM SISLGNYVSSLLV++VMKIST D + GWIP NLNKGHL
Sbjct: 489 LEFFNSQAPTGLKSFASALCMASISLGNYVSSLLVSIVMKISTTDDVHGWIPENLNKGHL 548

Query: 635 DRFYFLLAALTVVDFVIYIACAKWYKSIKLEDKYEQ--TEEPE 672
           +RFYFLLA LT  DFV+Y+ CAKWYK IK E  + +  TEE E
Sbjct: 549 ERFYFLLAGLTAADFVVYLICAKWYKYIKSEASFSESVTEEEE 588

BLAST of MC10g1294 vs. TAIR 10
Match: AT5G19640.1 (Major facilitator superfamily protein )

HSP 1 Score: 641.7 bits (1654), Expect = 6.5e-184
Identity = 327/550 (59.45%), Postives = 399/550 (72.55%), Query Frame = 0

Query: 114 KSGTWVAGIIILLNQGLATLAFFGVGVNLVLFLTRVLQQNNADAANNVSKWTGTVYIFSL 173
           K+G W   II+L+NQGLATLAFFGVGVNLVLFLTRV+ Q NA+AANNVSKWTGTVY+FSL
Sbjct: 59  KNGGWTNAIILLVNQGLATLAFFGVGVNLVLFLTRVMGQGNAEAANNVSKWTGTVYMFSL 118

Query: 174 VGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSLSSHLFLIRPKGCGDLQTPCGTHSKTEIS 233
           VGAFLSDSYWGRY TC IFQ+IFVIG+  LS  S  FLI+P+GCGD    C   S   ++
Sbjct: 119 VGAFLSDSYWGRYLTCTIFQVIFVIGVGLLSFVSWFFLIKPRGCGDGDLECNPPSSLGVA 178

Query: 234 LFYLSIYLTALGNGGYQPNIATFGADQFDEEDPKEGYSKVAFFSYFYLALNLGSLFSNTI 293
           +FYLS+YL A G GG+QP +ATFGADQ D++      SK AFFSYFY ALN+G+LFSNTI
Sbjct: 179 IFYLSVYLVAFGYGGHQPTLATFGADQLDDDK----NSKAAFFSYFYFALNVGALFSNTI 238

Query: 294 LGFFEDEGMWALGFWVSAASAVAALLLFLAGTSRYRHFKPTGNPLTRVCQVVVSAAKKWR 353
           L +FED+G+W  GF VS  SA+ AL+ FLA T +YR+ KP GNPL RV QV V+ A+KW 
Sbjct: 239 LVYFEDKGLWTEGFLVSLGSAIVALVAFLAPTRQYRYVKPCGNPLPRVAQVFVATARKWS 298

Query: 354 VRVPSGGEGLFEDDGKRSPDNGCRKILHTHGFKFLDKAAYISSRDLSDQEQGVCNPWRIC 413
           V  P     L+E +G  S   G RKI H+  F FLD+AA I+  D +       N WR+C
Sbjct: 299 VVRPGDPHELYELEGPESAIKGSRKIFHSTKFLFLDRAAVITENDRNGTRS---NAWRLC 358

Query: 414 PITQVEEVKCILRLLPIWLCTIIYSVVFTQMASLFVEQGAAMKTTISSFRIPPASMSSFD 473
            +TQVEE KC+++LLPIWLCTIIYSV+FTQMASLFVEQG  M   +  F IP ASMS FD
Sbjct: 359 SVTQVEEAKCVMKLLPIWLCTIIYSVIFTQMASLFVEQGDVMNAYVGKFHIPAASMSVFD 418

Query: 474 ILSVALFIFLYRRVLDPFVGKLKKSSSKGLTELQRMGVGLIIAVMAMVSAGIVECYRLKY 533
           I SV +   +YR ++ P+V           TEL RMG+GLII +MAMV+AG+ E  RLK 
Sbjct: 419 IFSVFVSTGIYRHIIFPYVRP---------TELMRMGIGLIIGIMAMVAAGLTEIQRLKR 478

Query: 534 AKADCTHCEGSSSLSIFWQVPQYALIGASEVFMYVGQLEFFNAQAPDGLKSFGSALCMTS 593
                   +  S L+I WQ+PQY L+GASEVFMYVGQLEFFN QAPDGLK+ GS+LCM S
Sbjct: 479 VVPG----QKESELTILWQIPQYVLVGASEVFMYVGQLEFFNGQAPDGLKNLGSSLCMAS 538

Query: 594 ISLGNYVSSLLVTMVMKIS-TVDRMPGWIPGNLNKGHLDRFYFLLAALTVVDFVIYIACA 653
           ++LGNYVSSL+V +VM I+   +  PGWIP NLN+GH+DRFYFL+AAL  +DFV+Y+  A
Sbjct: 539 MALGNYVSSLMVNIVMAITKRGENSPGWIPENLNEGHMDRFYFLIAALAAIDFVVYLIFA 588

Query: 654 KWYKSIKLED 663
           KWY+ I  ++
Sbjct: 599 KWYQPISHDE 588

BLAST of MC10g1294 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 486.9 bits (1252), Expect = 2.7e-137
Identity = 249/561 (44.39%), Postives = 371/561 (66.13%), Query Frame = 0

Query: 97  FTLDGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLAFFGVGVNLVLFLTRVLQQNNAD 156
           +T DGTVD H  PA + K+G W A   IL N+    LA++G+G NLV +L   L Q NA 
Sbjct: 7   YTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNAT 66

Query: 157 AANNVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSLSSHLFLIRPKG 216
           AANNV+ W+GT YI  L+GAF++D+Y GRY T A F  I+V G+  L+LS+ +  ++P  
Sbjct: 67  AANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGN 126

Query: 217 CGDLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEEDPKEGYSKVAFF 276
           C      C  +S ++ ++F++++Y+ ALG GG +P +++FGADQFDE D  E   K +FF
Sbjct: 127 CN--ADTCHPNS-SQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFF 186

Query: 277 SYFYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASAVAALLLFLAGTSRYRHFKPTGN 336
           ++FY ++N+G+L + T+L + +    W  GF V   + V A+  F  G+  YR  +P G+
Sbjct: 187 NWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGS 246

Query: 337 PLTRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDNGCRKILHTHGFKFLDKAAYISS 396
           PLTR+ QV+V+A +K  V+VP     LFE     S   G RK++HT   KF DKAA  S 
Sbjct: 247 PLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQ 306

Query: 397 RDLSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCTIIYSVVFTQMASLFVEQGAAMK 456
            D    + G  NPWR+C +TQVEE+K I+ LLP+W   I+++ V++QM+++FV QG  M 
Sbjct: 307 SD--SIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMD 366

Query: 457 TTI-SSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLTELQRMGVGLII 516
             +  +F IP AS+S FD +SV  +  +Y + + P   K  + + +G T+LQRMG+GL++
Sbjct: 367 QHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTR-NERGFTQLQRMGIGLVV 426

Query: 517 AVMAMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVPQYALIGASEVFMYVGQLEFFN 576
           ++ AM++AG++E  RL Y K    + +    +SIFWQ+PQY LIG +EVF ++GQLEFF 
Sbjct: 427 SIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFY 486

Query: 577 AQAPDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTVDRMPGWIPGNLNKGHLDRFYF 636
            QAPD ++S  SAL +T+++LGNY+S++LVT+VMKI+  +  PGWIP NLN+GHLD F++
Sbjct: 487 DQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFY 546

Query: 637 LLAALTVVDFVIYIACAKWYK 657
           LLA L+ ++F++Y+  +K YK
Sbjct: 547 LLATLSFLNFLVYLWISKRYK 561

BLAST of MC10g1294 vs. TAIR 10
Match: AT1G62200.1 (Major facilitator superfamily protein )

HSP 1 Score: 471.1 bits (1211), Expect = 1.5e-132
Identity = 238/559 (42.58%), Postives = 362/559 (64.76%), Query Frame = 0

Query: 100 DGTVDWHGRPAIRSKSGTWVAGIIILLNQGLATLAFFGVGVNLVLFLTRVLQQNNADAAN 159
           DG++D +G P  + K+G W A   IL N+    LA++G+  NL+ + T  L ++N  AA+
Sbjct: 38  DGSIDIYGNPPSKKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELHESNVSAAS 97

Query: 160 NVSKWTGTVYIFSLVGAFLSDSYWGRYKTCAIFQIIFVIGLVSLSLSSHLFLIRPKGC-G 219
           +V  W GT YI  L+GA ++DSYWGRY T A F  I+ IG+  L+LS+ L +++P  C G
Sbjct: 98  DVMIWQGTCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPVLKPAACAG 157

Query: 220 DLQTPCGTHSKTEISLFYLSIYLTALGNGGYQPNIATFGADQFDEEDPKEGYSKVAFFSY 279
                C   +  + ++F+  +YL ALG GG +P +++FGADQFD+ DP+E   K +FF++
Sbjct: 158 VAAALCSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDPRERVRKASFFNW 217

Query: 280 FYLALNLGSLFSNTILGFFEDEGMWALGFWVSAASAVAALLLFLAGTSRYRHFKPTGNPL 339
           FY ++N+GS  S+T+L + ++   W LGF +       ++  F  GT  YR  KP G+P+
Sbjct: 218 FYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTPLYRFQKPGGSPI 277

Query: 340 TRVCQVVVSAAKKWRVRVPSGGEGLFEDDGKRSPDNGCRKILHTHGFKFLDKAAYISSRD 399
           TRVCQV+V+A +K ++ +P     L+E   K S   G RKI HT G+KFLDKAA IS  +
Sbjct: 278 TRVCQVLVAAYRKLKLNLPEDISFLYETREKNSMIAGSRKIQHTDGYKFLDKAAVIS--E 337

Query: 400 LSDQEQGVCNPWRICPITQVEEVKCILRLLPIWLCTIIYSVVFTQMASLFVEQGAAMKTT 459
              +     NPW++C +TQVEEVK ++R+ PIW   I+YSV+++Q+++LFV+QG +M   
Sbjct: 338 YESKSGAFSNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTLFVQQGRSMNRI 397

Query: 460 ISSFRIPPASMSSFDILSVALFIFLYRRVLDPFVGKLKKSSSKGLTELQRMGVGLIIAVM 519
           I SF IPPAS   FD L V + I +Y R L PFV +      KGLT+LQRMG+GL ++V+
Sbjct: 398 IRSFEIPPASFGVFDTLIVLISIPIYDRFLVPFVRRF-TGIPKGLTDLQRMGIGLFLSVL 457

Query: 520 AMVSAGIVECYRLKYAKADCTHCEGSSSLSIFWQVPQYALIGASEVFMYVGQLEFFNAQA 579
           ++ +A IVE  RL+ A       +   ++SIFWQ+PQY L+G +EVF ++G++EFF  ++
Sbjct: 458 SIAAAAIVETVRLQLA-------QDFVAMSIFWQIPQYILMGIAEVFFFIGRVEFFYDES 517

Query: 580 PDGLKSFGSALCMTSISLGNYVSSLLVTMVMKISTVDRMPGWIPGNLNKGHLDRFYFLLA 639
           PD ++S  SAL + + ++G+Y+SSL++T+V   + +    GW+P +LNKGHLD F++LL 
Sbjct: 518 PDAMRSVCSALALLNTAVGSYLSSLILTLVAYFTALGGKDGWVPDDLNKGHLDYFFWLLV 577

Query: 640 ALTVVDFVIY-IACAKWYK 657
           +L +V+  +Y + C K  K
Sbjct: 578 SLGLVNIPVYALICVKHTK 586