MC10g1283 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g1283
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionprotein N-lysine methyltransferase METTL21A-like
LocationMC10: 16292221 .. 16292889 (+)
RNA-Seq ExpressionMC10g1283
SyntenyMC10g1283
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGTTAGAGAAATCCAAGTCTCTGATAGCAACATAATAATTAATGAGCTTGACGACCTCTATGATTCCACGACTGGCAGAGTGCTCACCGGTTCCTGGGTCTGGAACTCGGCGGTCGTCCTGGCTGAGTGGATGGCAACTCGGTGCAAACTCTTAGACTTCGACTTCAAAGACAAGACGGTGATAGAGCTCGGCGCTGGGGCTGGACTCCCTGGTTTGATGGCAGCTCTTCTTGGTGCCAGTCGAGTTGTACTTACCGATATAGAGTCGTTGCTGCCTGGGTTGTTGCAGAATGTAGATGCCAATGGACTTGGGGACCGGGTGGAAGTAAGGGAACTCGTCTGGGGTTCGGACAAATCGGTGAGTCAAGCCAACGAGTTGGGAGAGTTTGACCTGATATTGATGTCAGATGTGTTCTTCGACCAGGAGGAAATGCCCCCTCTAGCACAGATCCTAAAGATCATCGCTGGGGAAAATAGCATAATCTGGGCAGCATCCGAGCTCCGACCATGGACCATCGAGTGCCTTGGTGAGTTCACAAATCAAGGATTCAAGATTGACGAGACACCAGTTCAACTCGATCGTCCTTGTGAGGAAGACGACTATGCCATCTTAGACTCATTTTCCATTTTCCATCTCCAACCATCGGATGGAGAATGCAACTTT

mRNA sequence

ATGGGTGTTAGAGAAATCCAAGTCTCTGATAGCAACATAATAATTAATGAGCTTGACGACCTCTATGATTCCACGACTGGCAGAGTGCTCACCGGTTCCTGGGTCTGGAACTCGGCGGTCGTCCTGGCTGAGTGGATGGCAACTCGGTGCAAACTCTTAGACTTCGACTTCAAAGACAAGACGGTGATAGAGCTCGGCGCTGGGGCTGGACTCCCTGGTTTGATGGCAGCTCTTCTTGGTGCCAGTCGAGTTGTACTTACCGATATAGAGTCGTTGCTGCCTGGGTTGTTGCAGAATGTAGATGCCAATGGACTTGGGGACCGGGTGGAAGTAAGGGAACTCGTCTGGGGTTCGGACAAATCGGTGAGTCAAGCCAACGAGTTGGGAGAGTTTGACCTGATATTGATGTCAGATGTGTTCTTCGACCAGGAGGAAATGCCCCCTCTAGCACAGATCCTAAAGATCATCGCTGGGGAAAATAGCATAATCTGGGCAGCATCCGAGCTCCGACCATGGACCATCGAGTGCCTTGGTGAGTTCACAAATCAAGGATTCAAGATTGACGAGACACCAGTTCAACTCGATCGTCCTTGTGAGGAAGACGACTATGCCATCTTAGACTCATTTTCCATTTTCCATCTCCAACCATCGGATGGAGAATGCAACTTT

Coding sequence (CDS)

ATGGGTGTTAGAGAAATCCAAGTCTCTGATAGCAACATAATAATTAATGAGCTTGACGACCTCTATGATTCCACGACTGGCAGAGTGCTCACCGGTTCCTGGGTCTGGAACTCGGCGGTCGTCCTGGCTGAGTGGATGGCAACTCGGTGCAAACTCTTAGACTTCGACTTCAAAGACAAGACGGTGATAGAGCTCGGCGCTGGGGCTGGACTCCCTGGTTTGATGGCAGCTCTTCTTGGTGCCAGTCGAGTTGTACTTACCGATATAGAGTCGTTGCTGCCTGGGTTGTTGCAGAATGTAGATGCCAATGGACTTGGGGACCGGGTGGAAGTAAGGGAACTCGTCTGGGGTTCGGACAAATCGGTGAGTCAAGCCAACGAGTTGGGAGAGTTTGACCTGATATTGATGTCAGATGTGTTCTTCGACCAGGAGGAAATGCCCCCTCTAGCACAGATCCTAAAGATCATCGCTGGGGAAAATAGCATAATCTGGGCAGCATCCGAGCTCCGACCATGGACCATCGAGTGCCTTGGTGAGTTCACAAATCAAGGATTCAAGATTGACGAGACACCAGTTCAACTCGATCGTCCTTGTGAGGAAGACGACTATGCCATCTTAGACTCATTTTCCATTTTCCATCTCCAACCATCGGATGGAGAATGCAACTTT

Protein sequence

MGVREIQVSDSNIIINELDDLYDSTTGRVLTGSWVWNSAVVLAEWMATRCKLLDFDFKDKTVIELGAGAGLPGLMAALLGASRVVLTDIESLLPGLLQNVDANGLGDRVEVRELVWGSDKSVSQANELGEFDLILMSDVFFDQEEMPPLAQILKIIAGENSIIWAASELRPWTIECLGEFTNQGFKIDETPVQLDRPCEEDDYAILDSFSIFHLQPSDGECNF
Homology
BLAST of MC10g1283 vs. ExPASy Swiss-Prot
Match: A4FV98 (EEF1A lysine methyltransferase 3 OS=Bos taurus OX=9913 GN=EEF1AKMT3 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 5.4e-15
Identity = 50/137 (36.50%), Postives = 80/137 (58.39%), Query Frame = 0

Query: 35  VWNSAVVLAEWMATRCKLLDFDFKDKTVIELGAGAGLPGLMAALLGASRVVLTDIESLLP 94
           VW++A+ L  +  ++    + DF+ K VIELGAG G+ G++AAL G   V +TD+  +L 
Sbjct: 56  VWDAALSLCNYFESQ----NVDFRGKKVIELGAGTGIVGILAALQGGD-VTITDLPLVLE 115

Query: 95  GLLQNVDAN-GLGDRVEVRELVWGSDKSVSQANELGEFDLILMSDVFFDQEEMPPLAQIL 154
            +  NV AN   G R +VR L WG D+ V      G++DL+L +D+ + +   P L   L
Sbjct: 116 QIQGNVQANVPPGGRAQVRALSWGIDQHVFP----GDYDLVLGADIVYLEPTFPLLLGTL 175

Query: 155 KIIAGENSIIWAASELR 171
           + + G +  I+ AS++R
Sbjct: 176 RHLCGPHGTIYLASKMR 183

BLAST of MC10g1283 vs. ExPASy Swiss-Prot
Match: Q5BLD8 (Protein N-lysine methyltransferase METTL21A OS=Danio rerio OX=7955 GN=mettl21a PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.2e-14
Identity = 53/139 (38.13%), Postives = 80/139 (57.55%), Query Frame = 0

Query: 35  VWNSAVVLAEWMATRCKLLDFDFKDKTVIELGAGAGLPGLMAALLGASRVVLTDIESLLP 94
           VW++AVVL  ++    ++   D K K VIELGAG GL G++AALLGA+ V +TD E  L 
Sbjct: 46  VWDAAVVLCMFL----EMGKVDLKGKRVIELGAGTGLVGIVAALLGAN-VTITDREPALE 105

Query: 95  GLLQNVDAN---GLGDRVEVRELVWGSDKSVSQANELGEFDLILMSDVFFDQEEMPPLAQ 154
            L  NV  N   G    V+V EL WG +  +      G +DLIL +D+ + +E  P L Q
Sbjct: 106 FLTANVHENIPQGRQKAVQVSELTWGENLDLYPQ---GGYDLILGADIVYLEETFPALLQ 165

Query: 155 ILKIIAGENSIIWAASELR 171
            L+ ++  ++++  +  +R
Sbjct: 166 TLEHLSSGDTVVLLSCRIR 176

BLAST of MC10g1283 vs. ExPASy Swiss-Prot
Match: D3YWP0 (EEF1A lysine methyltransferase 3 OS=Mus musculus OX=10090 GN=eef1akmt3 PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.0e-13
Identity = 49/137 (35.77%), Postives = 77/137 (56.20%), Query Frame = 0

Query: 35  VWNSAVVLAEWMATRCKLLDFDFKDKTVIELGAGAGLPGLMAALLGASRVVLTDIESLLP 94
           VW++A+ L ++  ++    + DF+ K VIELGAG G+ G++AAL G   V +TD+   L 
Sbjct: 56  VWDAALSLCDYFESQ----NVDFRGKKVIELGAGTGIVGILAALQGGD-VTITDLPVALE 115

Query: 95  GLLQNVDAN-GLGDRVEVRELVWGSDKSVSQANELGEFDLILMSDVFFDQEEMPPLAQIL 154
            +  NV AN   G R  V  L WG D+ V      G +DL+L +D+ + +   P L   L
Sbjct: 116 QIQDNVHANVPPGGRARVCALSWGIDQHVFP----GNYDLVLGADIVYLEPTFPLLLGTL 175

Query: 155 KIIAGENSIIWAASELR 171
           + + G +  I+ AS++R
Sbjct: 176 RHLCGPHGTIYLASKMR 183

BLAST of MC10g1283 vs. ExPASy Swiss-Prot
Match: Q96AZ1 (EEF1A lysine methyltransferase 3 OS=Homo sapiens OX=9606 GN=EEF1AKMT3 PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.3e-13
Identity = 49/137 (35.77%), Postives = 77/137 (56.20%), Query Frame = 0

Query: 35  VWNSAVVLAEWMATRCKLLDFDFKDKTVIELGAGAGLPGLMAALLGASRVVLTDIESLLP 94
           VW++A+ L  +  ++    + DF+ K VIELGAG G+ G++AAL G   V +TD+   L 
Sbjct: 56  VWDAALSLCNYFESQ----NVDFRGKKVIELGAGTGIVGILAALQGGD-VTITDLPLALE 115

Query: 95  GLLQNVDAN-GLGDRVEVRELVWGSDKSVSQANELGEFDLILMSDVFFDQEEMPPLAQIL 154
            +  NV AN   G + +VR L WG D  V  AN    +DL+L +D+ + +   P L   L
Sbjct: 116 QIQGNVQANVPAGGQAQVRALSWGIDHHVFPAN----YDLVLGADIVYLEPTFPLLLGTL 175

Query: 155 KIIAGENSIIWAASELR 171
           + +   +  I+ AS++R
Sbjct: 176 QHLCRPHGTIYLASKMR 183

BLAST of MC10g1283 vs. ExPASy Swiss-Prot
Match: Q8WXB1 (Protein N-lysine methyltransferase METTL21A OS=Homo sapiens OX=9606 GN=METTL21A PE=1 SV=2)

HSP 1 Score: 78.2 bits (191), Expect = 1.3e-13
Identity = 49/139 (35.25%), Postives = 80/139 (57.55%), Query Frame = 0

Query: 35  VWNSAVVLAEWMATRCKLLDFDFKDKTVIELGAGAGLPGLMAALLGASRVVLTDIESLLP 94
           VW++A+VL+ ++    ++   + + ++ +ELGAG GL G++AALLGA  V +TD +  L 
Sbjct: 46  VWDAAIVLSTYL----EMGAVELRGRSAVELGAGTGLVGIVAALLGA-HVTITDRKVALE 105

Query: 95  GLLQNVDAN---GLGDRVEVRELVWGSDKSVSQANELGEFDLILMSDVFFDQEEMPPLAQ 154
            L  NV AN    +  +  V+EL WG +     +   GEFDLIL +D+ + +E    L Q
Sbjct: 106 FLKSNVQANLPPHIQTKTVVKELTWGQNLG---SFSPGEFDLILGADIIYLEETFTDLLQ 165

Query: 155 ILKIIAGENSIIWAASELR 171
            L+ +   +S+I  A  +R
Sbjct: 166 TLEHLCSNHSVILLACRIR 176

BLAST of MC10g1283 vs. NCBI nr
Match: XP_022155530.1 (protein N-lysine methyltransferase METTL21A-like [Momordica charantia])

HSP 1 Score: 453 bits (1166), Expect = 6.85e-161
Identity = 223/223 (100.00%), Postives = 223/223 (100.00%), Query Frame = 0

Query: 1   MGVREIQVSDSNIIINELDDLYDSTTGRVLTGSWVWNSAVVLAEWMATRCKLLDFDFKDK 60
           MGVREIQVSDSNIIINELDDLYDSTTGRVLTGSWVWNSAVVLAEWMATRCKLLDFDFKDK
Sbjct: 1   MGVREIQVSDSNIIINELDDLYDSTTGRVLTGSWVWNSAVVLAEWMATRCKLLDFDFKDK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDIESLLPGLLQNVDANGLGDRVEVRELVWGSDK 120
           TVIELGAGAGLPGLMAALLGASRVVLTDIESLLPGLLQNVDANGLGDRVEVRELVWGSDK
Sbjct: 61  TVIELGAGAGLPGLMAALLGASRVVLTDIESLLPGLLQNVDANGLGDRVEVRELVWGSDK 120

Query: 121 SVSQANELGEFDLILMSDVFFDQEEMPPLAQILKIIAGENSIIWAASELRPWTIECLGEF 180
           SVSQANELGEFDLILMSDVFFDQEEMPPLAQILKIIAGENSIIWAASELRPWTIECLGEF
Sbjct: 121 SVSQANELGEFDLILMSDVFFDQEEMPPLAQILKIIAGENSIIWAASELRPWTIECLGEF 180

Query: 181 TNQGFKIDETPVQLDRPCEEDDYAILDSFSIFHLQPSDGECNF 223
           TNQGFKIDETPVQLDRPCEEDDYAILDSFSIFHLQPSDGECNF
Sbjct: 181 TNQGFKIDETPVQLDRPCEEDDYAILDSFSIFHLQPSDGECNF 223

BLAST of MC10g1283 vs. NCBI nr
Match: KAG7015681.1 (EEF1A lysine methyltransferase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 372 bits (956), Expect = 7.28e-129
Identity = 180/223 (80.72%), Postives = 202/223 (90.58%), Query Frame = 0

Query: 1   MGVREIQVSDSNIIINELDDLYDSTTGRVLTGSWVWNSAVVLAEWMATRCKLLDFDFKDK 60
           MGVRE Q+SD+N++I+ELDDLYDSTTGRVLTGSWVWNSA VLAEWMATRCKL DFDF+DK
Sbjct: 1   MGVREFQISDTNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATRCKLFDFDFRDK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDIESLLPGLLQNVDANGLGDRVEVRELVWGSDK 120
           TVIELGAGAGLPGLMAALLGA RVVLTD+E LLPGLL+NVDANGL DRVEVRELVWG D+
Sbjct: 61  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 120

Query: 121 SVSQANELGEFDLILMSDVFFDQEEMPPLAQILKIIAGENSIIWAASELRPWTIECLGEF 180
           SVS+ANE GEFDLILMSD+F+D E+M PLA ILKII+G+NS IWAASE+RPWT+ECL EF
Sbjct: 121 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKNSKIWAASEIRPWTVECLTEF 180

Query: 181 TNQGFKIDETPVQLDRPCEE-DDYAILDSFSIFHLQPSDGECN 222
            N+GFK++E+ VQLD P EE DD+AILDSFSIFHLQP D E N
Sbjct: 181 MNEGFKVEESQVQLDEPREEVDDHAILDSFSIFHLQPPDEERN 223

BLAST of MC10g1283 vs. NCBI nr
Match: XP_022923423.1 (protein N-lysine methyltransferase METTL21A-like [Cucurbita moschata])

HSP 1 Score: 372 bits (954), Expect = 1.47e-128
Identity = 179/223 (80.27%), Postives = 202/223 (90.58%), Query Frame = 0

Query: 1   MGVREIQVSDSNIIINELDDLYDSTTGRVLTGSWVWNSAVVLAEWMATRCKLLDFDFKDK 60
           MGVRE Q+SD+N++I+ELDDLYDSTTGRVLTGSWVWNSA VLAEWMAT+CKL DFDF+DK
Sbjct: 1   MGVREFQISDTNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDFRDK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDIESLLPGLLQNVDANGLGDRVEVRELVWGSDK 120
           TVIELGAGAGLPGLMAALLGA RVVLTD+E LLPGLL+NVDANGL DRVEVRELVWG D+
Sbjct: 61  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 120

Query: 121 SVSQANELGEFDLILMSDVFFDQEEMPPLAQILKIIAGENSIIWAASELRPWTIECLGEF 180
           SVS+ANE GEFDLILMSD+F+D E+M PLA ILKII+G+NS IWAASE+RPWT+ECL EF
Sbjct: 121 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKNSKIWAASEIRPWTVECLTEF 180

Query: 181 TNQGFKIDETPVQLDRPCEEDD-YAILDSFSIFHLQPSDGECN 222
            N+GFK++E+ VQLD P EEDD +AILDSFSIFHLQP D E N
Sbjct: 181 MNEGFKVEESQVQLDEPSEEDDDHAILDSFSIFHLQPPDEERN 223

BLAST of MC10g1283 vs. NCBI nr
Match: KAG6577643.1 (EEF1A lysine methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 369 bits (947), Expect = 1.71e-127
Identity = 178/223 (79.82%), Postives = 201/223 (90.13%), Query Frame = 0

Query: 1   MGVREIQVSDSNIIINELDDLYDSTTGRVLTGSWVWNSAVVLAEWMATRCKLLDFDFKDK 60
           MGVRE Q+SD+N++I+ELDDLYDSTTGRVLTGSWVWNSA VLAEWMAT+CKL DFDF+DK
Sbjct: 1   MGVREFQISDTNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDFRDK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDIESLLPGLLQNVDANGLGDRVEVRELVWGSDK 120
           TVIELGAGAGLPGLMAALLGA  VVLTD+E LLPGLL+NVDANGL DRVEVRELVWG D+
Sbjct: 61  TVIELGAGAGLPGLMAALLGARHVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 120

Query: 121 SVSQANELGEFDLILMSDVFFDQEEMPPLAQILKIIAGENSIIWAASELRPWTIECLGEF 180
           SVS+ANE GEFDLILMSD+F+D E+M PLA ILKII+G+NS IWAASE+RPWT+ECL EF
Sbjct: 121 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKNSKIWAASEIRPWTVECLTEF 180

Query: 181 TNQGFKIDETPVQLDRPCEE-DDYAILDSFSIFHLQPSDGECN 222
            N+GFK++E+ VQLD P EE DD+AILDSFSIFHLQP D E N
Sbjct: 181 MNEGFKVEESQVQLDEPREEVDDHAILDSFSIFHLQPPDEERN 223

BLAST of MC10g1283 vs. NCBI nr
Match: XP_023553140.1 (protein N-lysine methyltransferase METTL21A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 369 bits (947), Expect = 3.86e-127
Identity = 178/223 (79.82%), Postives = 201/223 (90.13%), Query Frame = 0

Query: 1   MGVREIQVSDSNIIINELDDLYDSTTGRVLTGSWVWNSAVVLAEWMATRCKLLDFDFKDK 60
           MGVRE Q+SD N++I+ELDDLYDSTTGRVLTGSWVWNSA VLAEWMAT+CKL DFDF+DK
Sbjct: 24  MGVREFQISDRNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDFRDK 83

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDIESLLPGLLQNVDANGLGDRVEVRELVWGSDK 120
           TVIELGAGAGLPGLMAALLGA RVVLTD+E LLPGLL+NVDANGL DRVEVRELVWG D+
Sbjct: 84  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 143

Query: 121 SVSQANELGEFDLILMSDVFFDQEEMPPLAQILKIIAGENSIIWAASELRPWTIECLGEF 180
           SVS+ANE GEFDLILMSD+F+D E+M PLA ILKII+G++S IWAASE+RPWT+ECL EF
Sbjct: 144 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKHSKIWAASEIRPWTVECLTEF 203

Query: 181 TNQGFKIDETPVQLDRPCEEDD-YAILDSFSIFHLQPSDGECN 222
           TN+GFK++E+ VQLD P EEDD + ILDSFSIFHLQP D E N
Sbjct: 204 TNEGFKVEESQVQLDEPREEDDDHTILDSFSIFHLQPPDEERN 246

BLAST of MC10g1283 vs. ExPASy TrEMBL
Match: A0A6J1DMN9 (protein N-lysine methyltransferase METTL21A-like OS=Momordica charantia OX=3673 GN=LOC111022654 PE=4 SV=1)

HSP 1 Score: 453 bits (1166), Expect = 3.32e-161
Identity = 223/223 (100.00%), Postives = 223/223 (100.00%), Query Frame = 0

Query: 1   MGVREIQVSDSNIIINELDDLYDSTTGRVLTGSWVWNSAVVLAEWMATRCKLLDFDFKDK 60
           MGVREIQVSDSNIIINELDDLYDSTTGRVLTGSWVWNSAVVLAEWMATRCKLLDFDFKDK
Sbjct: 1   MGVREIQVSDSNIIINELDDLYDSTTGRVLTGSWVWNSAVVLAEWMATRCKLLDFDFKDK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDIESLLPGLLQNVDANGLGDRVEVRELVWGSDK 120
           TVIELGAGAGLPGLMAALLGASRVVLTDIESLLPGLLQNVDANGLGDRVEVRELVWGSDK
Sbjct: 61  TVIELGAGAGLPGLMAALLGASRVVLTDIESLLPGLLQNVDANGLGDRVEVRELVWGSDK 120

Query: 121 SVSQANELGEFDLILMSDVFFDQEEMPPLAQILKIIAGENSIIWAASELRPWTIECLGEF 180
           SVSQANELGEFDLILMSDVFFDQEEMPPLAQILKIIAGENSIIWAASELRPWTIECLGEF
Sbjct: 121 SVSQANELGEFDLILMSDVFFDQEEMPPLAQILKIIAGENSIIWAASELRPWTIECLGEF 180

Query: 181 TNQGFKIDETPVQLDRPCEEDDYAILDSFSIFHLQPSDGECNF 223
           TNQGFKIDETPVQLDRPCEEDDYAILDSFSIFHLQPSDGECNF
Sbjct: 181 TNQGFKIDETPVQLDRPCEEDDYAILDSFSIFHLQPSDGECNF 223

BLAST of MC10g1283 vs. ExPASy TrEMBL
Match: A0A6J1E9L9 (protein N-lysine methyltransferase METTL21A-like OS=Cucurbita moschata OX=3662 GN=LOC111431122 PE=4 SV=1)

HSP 1 Score: 372 bits (954), Expect = 7.11e-129
Identity = 179/223 (80.27%), Postives = 202/223 (90.58%), Query Frame = 0

Query: 1   MGVREIQVSDSNIIINELDDLYDSTTGRVLTGSWVWNSAVVLAEWMATRCKLLDFDFKDK 60
           MGVRE Q+SD+N++I+ELDDLYDSTTGRVLTGSWVWNSA VLAEWMAT+CKL DFDF+DK
Sbjct: 1   MGVREFQISDTNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDFRDK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDIESLLPGLLQNVDANGLGDRVEVRELVWGSDK 120
           TVIELGAGAGLPGLMAALLGA RVVLTD+E LLPGLL+NVDANGL DRVEVRELVWG D+
Sbjct: 61  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 120

Query: 121 SVSQANELGEFDLILMSDVFFDQEEMPPLAQILKIIAGENSIIWAASELRPWTIECLGEF 180
           SVS+ANE GEFDLILMSD+F+D E+M PLA ILKII+G+NS IWAASE+RPWT+ECL EF
Sbjct: 121 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKNSKIWAASEIRPWTVECLTEF 180

Query: 181 TNQGFKIDETPVQLDRPCEEDD-YAILDSFSIFHLQPSDGECN 222
            N+GFK++E+ VQLD P EEDD +AILDSFSIFHLQP D E N
Sbjct: 181 MNEGFKVEESQVQLDEPSEEDDDHAILDSFSIFHLQPPDEERN 223

BLAST of MC10g1283 vs. ExPASy TrEMBL
Match: A0A6J1HN15 (protein N-lysine methyltransferase METTL21A-like OS=Cucurbita maxima OX=3661 GN=LOC111465095 PE=4 SV=1)

HSP 1 Score: 363 bits (933), Expect = 3.24e-125
Identity = 175/223 (78.48%), Postives = 199/223 (89.24%), Query Frame = 0

Query: 1   MGVREIQVSDSNIIINELDDLYDSTTGRVLTGSWVWNSAVVLAEWMATRCKLLDFDFKDK 60
           MGVRE Q+SD N++I+ELDDLYDSTTGRVLTGSWVWNSA VLAEWMAT+CKL DFD +DK
Sbjct: 31  MGVREFQISDRNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDIRDK 90

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDIESLLPGLLQNVDANGLGDRVEVRELVWGSDK 120
           TVIELGAG GLPGLMAALLGA RVVLTD+E LLPGLL+NVDANGL DRV+VRELVWG D+
Sbjct: 91  TVIELGAGVGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLVDRVQVRELVWGLDE 150

Query: 121 SVSQANELGEFDLILMSDVFFDQEEMPPLAQILKIIAGENSIIWAASELRPWTIECLGEF 180
           SVS+ANELGEFDLILMSD+F+D E+M PLA +LKII+G+NS IWAASE+RPWT+ECL EF
Sbjct: 151 SVSRANELGEFDLILMSDLFYDSEDMAPLAHVLKIISGKNSKIWAASEIRPWTVECLKEF 210

Query: 181 TNQGFKIDETPVQLDRPCEEDD-YAILDSFSIFHLQPSDGECN 222
            N+GFK++E+ VQLD P EEDD +AILD FSIFHLQP D E N
Sbjct: 211 MNEGFKVEESQVQLDEPREEDDDHAILDLFSIFHLQPPDEERN 253

BLAST of MC10g1283 vs. ExPASy TrEMBL
Match: A0A0A0L6N4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G016950 PE=4 SV=1)

HSP 1 Score: 338 bits (868), Expect = 8.04e-116
Identity = 162/215 (75.35%), Postives = 188/215 (87.44%), Query Frame = 0

Query: 1   MGVREIQVSDSNIIINELDDLYDSTTGRVLTGSWVWNSAVVLAEWMATRCKLLDFDFKDK 60
           M V+E Q+SD NI+I+ELDDLYDSTTGRVLTGSWVWNSA VLA+WMAT+C L DFDF+ K
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDIESLLPGLLQNVDANGLGDRVEVRELVWGSDK 120
            VIELGAG GLPGL AALLGA+RV+LTD+E LLPGLL+NVDANG+GDRVEVRELVWGS+ 
Sbjct: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120

Query: 121 SVSQANELGEFDLILMSDVFFDQEEMPPLAQILKIIAGENSIIWAASELRPWTIECLGEF 180
             SQANELGEFDLILMSD+F++ EEMP LAQ+LKII+G ++ IWAASE+RPWTIEC+ E 
Sbjct: 121 LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL 180

Query: 181 TNQGFKIDETPVQLDRPCEEDDYAILDSFSIFHLQ 215
            NQGFKI E+PVQ D   EED++ ILDSFSIFHL+
Sbjct: 181 INQGFKITESPVQHDESHEEDNHKILDSFSIFHLR 215

BLAST of MC10g1283 vs. ExPASy TrEMBL
Match: A0A5A7UCU5 (Protein N-lysine methyltransferase METTL21A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold344G001920 PE=4 SV=1)

HSP 1 Score: 333 bits (855), Expect = 7.94e-114
Identity = 163/216 (75.46%), Postives = 188/216 (87.04%), Query Frame = 0

Query: 1   MGVREIQVSDSNIIINELDDLYDSTTGRVLTGSWVWNSAVVLAEWMATRCKLLDFDFKDK 60
           M V+E Q+SD NI+I+ELDDLYDSTTGRVLTGSWVWNSA VLAEWMAT+CKL DFD + K
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDLRQK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDIESLLPGLLQNVDANGLGDRVEVRELVWGSDK 120
            VIELGAGAGLPGL AALLGA+RVVLTD++ LL GLL+NVDAN LGDRVEVRELVWGS++
Sbjct: 61  NVIELGAGAGLPGLTAALLGANRVVLTDVKPLLLGLLENVDANRLGDRVEVRELVWGSNE 120

Query: 121 SVSQANELGEFDLILMSDVFFDQEEMPPLAQILKIIAGENSIIWAASELRPWTIECLGEF 180
             SQANELGEFDLILMSD+F+D EEMP LAQ+LKII+G +S IWAASE+RPWTIEC+ E 
Sbjct: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDSKIWAASEIRPWTIECITEL 180

Query: 181 TNQGFKIDETPVQLDRPC-EEDDYAILDSFSIFHLQ 215
            NQGFKI E+PVQ D    EED++ +LDS+SIFHL+
Sbjct: 181 INQGFKITESPVQHDDESHEEDNHKVLDSYSIFHLR 216

BLAST of MC10g1283 vs. TAIR 10
Match: AT5G44170.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 77.0 bits (188), Expect = 2.1e-14
Identity = 54/199 (27.14%), Postives = 96/199 (48.24%), Query Frame = 0

Query: 27  GRVLTGSWVWNSAVVLAEWMATRCKLLD--------------FDFKDKTVIELGAGAGLP 86
           G +  G+ VW  +++L+++ A R   LD              FDF+ +  IELG G G+ 
Sbjct: 25  GSMHVGTSVWPCSLILSKF-AERWSTLDSSSSTTSPNPYAELFDFRRRRGIELGTGCGVA 84

Query: 87  GLMAALLGASRVVLTDIESLLPGLLQNVDAN--GLGDRVEVRELVWGSDKSVSQANELGE 146
           G+   LLG + +VLTDI  ++P L  N+  N   LG  ++   + W +   +S       
Sbjct: 85  GMAFYLLGLTEIVLTDIAPVMPALKHNLKRNKTALGKSLKTSIVYWNNRDQISALKP--P 144

Query: 147 FDLILMSDVFFDQEEMPPLAQILKIIAGENSIIWAASELR-PWTIECLGEFTNQGFKIDE 206
           FDL++ +DV + +E +  L   ++++  ++  +    ++R P   +   E  +  FKI++
Sbjct: 145 FDLVIAADVVYIEESVGQLVTAMELLVADDGAVLLGYQIRSPEADKLFWELCDIVFKIEK 204

BLAST of MC10g1283 vs. TAIR 10
Match: AT1G73320.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 74.3 bits (181), Expect = 1.4e-13
Identity = 48/145 (33.10%), Postives = 74/145 (51.03%), Query Frame = 0

Query: 30  LTGSWVWNSAVVLAEWMATRCKLLDFDFKDKTVIELGAGAGLPGLMAALLGASRVVLTDI 89
           +TGS +W+S VVL +++           + K ++ELG+G GL G +AALLG +  VLTD+
Sbjct: 117 VTGSVMWDSGVVLGKFLEHSVDSKVLSLEGKKIVELGSGCGLVGCIAALLGGN-AVLTDL 176

Query: 90  ESLLPGLLQNVDAN----GLGDRVEVRELVWGSDKSVSQANELGEFDLILMSDVFFDQEE 149
              L  L +N+  N           V+ELVWG D            D +L SDV + +E 
Sbjct: 177 PDRLRLLKKNIQTNLHRGNTRGSAIVQELVWGDDPDPDLIEPFP--DYVLGSDVIYSEEA 236

Query: 150 MPPLAQILKIIAGENSIIWAASELR 171
           +  L + L  +  + + I+ + ELR
Sbjct: 237 VHHLVKTLLQLCSDQTTIFLSGELR 258

BLAST of MC10g1283 vs. TAIR 10
Match: AT1G08125.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 71.6 bits (174), Expect = 8.8e-13
Identity = 50/193 (25.91%), Postives = 94/193 (48.70%), Query Frame = 0

Query: 13  IIINELDDLYDSTTGRVLTGSWVWNSAVVLAEWMATRCKLLDFD---FKDKTVIELGAGA 72
           ++ +ELD   D  +  +  G+ VW++++V A+++    +   F     K K  IELGAG 
Sbjct: 18  VLGHELDFAQDPNSKHL--GTTVWDASMVFAKYLGKNSRKGRFSSSKLKGKRAIELGAGC 77

Query: 73  GLPGLMAALLGASRVVLTDIESLLPGLLQNVDAN----------GLGDRVEVRELVWGSD 132
           G+ G   A+LG   VV TD + +LP L +NV+ N               + V EL WG++
Sbjct: 78  GVAGFALAMLGCD-VVTTDQKEVLPLLKRNVEWNTSRIVQMNPGSAFGSLRVAELDWGNE 137

Query: 133 KSVSQANELGEFDLILMSDVFFDQEEMPPLAQILKIIAGENSIIWAASELRPWTI-ECLG 192
             ++       FD ++ +DV + ++ + PL + +  ++G  + +    E+R   + E + 
Sbjct: 138 DHITAVEP--PFDYVIGTDVVYSEQLLEPLLRTILALSGPKTTVMLGYEIRSTVVHEKML 197

BLAST of MC10g1283 vs. TAIR 10
Match: AT1G08125.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 71.6 bits (174), Expect = 8.8e-13
Identity = 50/193 (25.91%), Postives = 94/193 (48.70%), Query Frame = 0

Query: 13  IIINELDDLYDSTTGRVLTGSWVWNSAVVLAEWMATRCKLLDFD---FKDKTVIELGAGA 72
           ++ +ELD   D  +  +  G+ VW++++V A+++    +   F     K K  IELGAG 
Sbjct: 25  VLGHELDFAQDPNSKHL--GTTVWDASMVFAKYLGKNSRKGRFSSSKLKGKRAIELGAGC 84

Query: 73  GLPGLMAALLGASRVVLTDIESLLPGLLQNVDAN----------GLGDRVEVRELVWGSD 132
           G+ G   A+LG   VV TD + +LP L +NV+ N               + V EL WG++
Sbjct: 85  GVAGFALAMLGCD-VVTTDQKEVLPLLKRNVEWNTSRIVQMNPGSAFGSLRVAELDWGNE 144

Query: 133 KSVSQANELGEFDLILMSDVFFDQEEMPPLAQILKIIAGENSIIWAASELRPWTI-ECLG 192
             ++       FD ++ +DV + ++ + PL + +  ++G  + +    E+R   + E + 
Sbjct: 145 DHITAVEP--PFDYVIGTDVVYSEQLLEPLLRTILALSGPKTTVMLGYEIRSTVVHEKML 204

BLAST of MC10g1283 vs. TAIR 10
Match: AT3G50850.1 (Putative methyltransferase family protein )

HSP 1 Score: 61.2 bits (147), Expect = 1.2e-09
Identity = 38/124 (30.65%), Postives = 63/124 (50.81%), Query Frame = 0

Query: 62  VIELGAGAGLPGLMAALLGASRVVLTDIESLLPGLLQNVDANG-----LGDRVEVRELVW 121
           ++ELG+G G+ G+ AA    + V +TD+ +++  L  N DAN       G +V V  L W
Sbjct: 92  IVELGSGTGIVGIAAAATLGANVTVTDLPNVIENLKFNADANAQVVAKFGGKVHVASLRW 151

Query: 122 GSDKSVSQANELGE-FDLILMSDVFFDQEEMPPLAQILK--IIAGENSIIWAASELRPWT 178
           G    +     LG+  DLIL SDV +      PL + L+  ++ G +  ++  + L+ W 
Sbjct: 152 G---EIDDVESLGQNVDLILASDVVYHVHLYEPLLKTLRFLLLEGSSERVFLMAHLKRWK 211

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A4FV985.4e-1536.50EEF1A lysine methyltransferase 3 OS=Bos taurus OX=9913 GN=EEF1AKMT3 PE=2 SV=1[more]
Q5BLD81.2e-1438.13Protein N-lysine methyltransferase METTL21A OS=Danio rerio OX=7955 GN=mettl21a P... [more]
D3YWP01.0e-1335.77EEF1A lysine methyltransferase 3 OS=Mus musculus OX=10090 GN=eef1akmt3 PE=3 SV=1[more]
Q96AZ11.3e-1335.77EEF1A lysine methyltransferase 3 OS=Homo sapiens OX=9606 GN=EEF1AKMT3 PE=1 SV=1[more]
Q8WXB11.3e-1335.25Protein N-lysine methyltransferase METTL21A OS=Homo sapiens OX=9606 GN=METTL21A ... [more]
Match NameE-valueIdentityDescription
XP_022155530.16.85e-161100.00protein N-lysine methyltransferase METTL21A-like [Momordica charantia][more]
KAG7015681.17.28e-12980.72EEF1A lysine methyltransferase 3, partial [Cucurbita argyrosperma subsp. argyros... [more]
XP_022923423.11.47e-12880.27protein N-lysine methyltransferase METTL21A-like [Cucurbita moschata][more]
KAG6577643.11.71e-12779.82EEF1A lysine methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023553140.13.86e-12779.82protein N-lysine methyltransferase METTL21A-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1DMN93.32e-161100.00protein N-lysine methyltransferase METTL21A-like OS=Momordica charantia OX=3673 ... [more]
A0A6J1E9L97.11e-12980.27protein N-lysine methyltransferase METTL21A-like OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1HN153.24e-12578.48protein N-lysine methyltransferase METTL21A-like OS=Cucurbita maxima OX=3661 GN=... [more]
A0A0A0L6N48.04e-11675.35Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G016950 PE=4 SV=1[more]
A0A5A7UCU57.94e-11475.46Protein N-lysine methyltransferase METTL21A-like OS=Cucumis melo var. makuwa OX=... [more]
Match NameE-valueIdentityDescription
AT5G44170.12.1e-1427.14S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G73320.11.4e-1333.10S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G08125.18.8e-1325.91S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G08125.28.8e-1325.91S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G50850.11.2e-0930.65Putative methyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 18..213
e-value: 5.1E-43
score: 149.2
NoneNo IPR availablePANTHERPTHR14614:SF123OSJNBA0070O11.4 PROTEINcoord: 1..218
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 61..157
e-value: 2.2528E-6
score: 43.1875
IPR019410Lysine methyltransferasePFAMPF10294Methyltransf_16coord: 31..170
e-value: 2.8E-27
score: 95.6
IPR019410Lysine methyltransferasePANTHERPTHR14614HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGENcoord: 1..218
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 36..168

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g1283.1MC10g1283.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008168 methyltransferase activity