MC10g1066 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g1066
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionGDSL esterase/lipase At5g55050-like
LocationMC10: 10977316 .. 10983567 (+)
RNA-Seq ExpressionMC10g1066
SyntenyMC10g1066
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CTAGACTCTAATAAGAAGAGAAGTTATGGAAAGAAAATGGGAACAGACAAATAATGGACAACTTGGTCCAGAAGAGAATGATTATTAAATATGAATAGAAATTCCCCACACATATCCACATGGGTCCCCACCCAACCCATCAACATACTCCTCTTCTTCTCTTACTTTTTCTAATTTTCACACTCAATTTTGGATTCTCATCTCTCTCTCTCTCTCTCTATATAAATTCCCTTTCAAGTTTCAATATTCCATTGAAGAAAAAGGAAATGGGTTCCAGAGCCTTCTTCTTCTTCTTCATCTTCTTGTGTGTATCATTCAGTTTTAGAGTTTGTGAATGTGGTGCCTTAGTTCCTGCAATCTATGTCTTCGGAGACTCCTTGGTTGATGTCGGCAACAACAACCACTTGAAGCTCTCTCTCGCCAAAGCCAACTTCCCTCACAATGGAATCGACTTCCCCACTACTAAACCCACCGGAAGGTTCTCTAATGGCAAGAACGCTGCCGACTATCTTGGTACGTAACATCCTACTAGTCTCTATCATATTTGAACTCACGCACTTTTAGTTAGAATATGCTGTCTTAATTAGTTGAGTTGTATTAAGGTTTTGCACCGTAGTGATTAAGATACTACGATTTAAACAAATAGGACAAATTTAGTTGGAATATGTCTTAATTAGTTGAGTTAGGTTAAAGTTTCGCACCATAATTGATATCTTATATTCCTAATAAAAATATGACAAGTTATTAGGATATCACAGTTTAAATAAATAGGAAAATTCAATGTAGAAAGGTGCCAAACTTGAACCTATATTTCAATTGATATCATATACTAAAAATAAAAATTTATAATAAAACATGGGGACAGGTGAGTATCTATCCTTGTCTTGTCACACGAGTGACTTTGGTTTGATCCTCCACGTTTTTTTTTTAAAGTAACGACTTTTTAGTTGGAGATACACATCTTAATTAGTTGAGCTGATTTTATGTTCGTGAATAGAGAATTTTTGTTTTGACTAAAAAAACCCATGAAAAAGTTATTAAACTCTTTATTAATGAAATCATGCTTATCTGTCTTTTTTTCCTTTCAGAAACTGTTATTTTTGGGTTTTTTTTTCCCTCTTTGAATATTATTTATAAAAAGTAGTGAATAGAAGTTACATGATGCATCCCACTGGTTTGGTTCTATTCTTAATGACATGGCAACCGAATTAAATAATGTTTTCTATTTGTCATGTACTATTATTCCATTTTCTTATATTGTTGCTACCATGCCATCAAAATTCTCTTCCTTTCCTTCTAGTAAAAGATATGGCACATGCCTTTTTTATATACTTATTTTAAAATCTTTAATTAATATTGTAAATTTTATTATAACAAAATCTGAACCCTTAATATTTTTATAAAGAAATAAAATAACCCTCCCTTGCCTATTTGTTTTTAGATAGTTGCTAATGCAAGTATAGCTTAATTTCTTTATTTTCGAGATTAGAAGTTCAAATTCACGTAATAAAGTTATATATGTGTGTTTTAGACCGCGGACAACAATATGATATGGGATGAACTCATGTCCATCTACGCCAAACCCGTAAAACCTATTTTCTTTTATTTTAGTCTTTTAATTTTACTTTAACTTTAAGTTTAAGTTTCTTTTTTTTTTTGAGAAAATGTTTTATGTTATTTTATTGTTATAATATCCTGTATAATCTCTGTTGAGTCATAATTTCTTAAATAATTATCTCGTAGTTCAATTTACTAGGACCGTCTTATTTTTGTTATTCATTTTAAATTTTTGAAAAACATTGATATTTGATCATTATTATTGTTTCTCGTTTTTCATCTTTAAAAAATATTTTTAAAATTGTATAGAAATTTTTTAAATAGGATTCTTGTTTTTCATTTTTCAAAATATGGAAAAAGTATAATTTTATCCTTCTTAATTTATTAAAGGCATAATAAATACTTAAAAAGTAAACATAACATAAAAATTTTGAATACCTAATTTAAAATTTTAACTTATTTTATTTAATTTAAAATTCTAGAGAACAGGATACAAGAAAAGACAATCAAATATATTTCTGTTCTTTTTTTCCTAACAGAAAAGAAAGAAAAAGAAAAAAAAACAATTAGAAAAATGCAGTTATACTTTTTTTTTAAAAGGAAGAAGTAAGAAAAATAAATGTTACGGAGCAAATCCATGATATCTCACTCTCAATAATTTTATTAATTAGTTAGTTAATGTATTAAGGTTTTTTTCTAATATTACCAATTATTTAAACAGAACTCAATCAGATAATAAATTAATAACCTCTATTTATGTCAAAAAAAATCCCTCTTTTATGGGTTGAGACTTTGACTTTGTAATATTATTATTCTAAAAAATATATATTTTAAACCGTCAGAAACACTCGAACATTATTTACTGATCATAAATGCATGCCAAATAATATCTATAAATCTTATTATCAGAGAGAAAATTCTCCTTTAATTATAAGGTCTTGGATGATGTGTTTTTCTTTGAATTGCAAGAAATCACCTCCCTTAGCTTGGCAGGAAAATATTTAATTAATACATATAAAACTTTTAAAAGTACCAAAATAATTCTTCATGACGAAAAGTTTCCATTCACATTAATGGGCAAAAGAAAAAGAAAAAAAAAACATAAAGAAAGACTACAAAATACTAATATATTTGATGATGTGTGTTTTTCTTTTATATACATTTTAATTTGATGTAATCAATGAAATTTTGAACAGCTGAGAAAGTAGGCCTACCGACTTCACCACCGTATCTCTCCCTAGTATCCAAGTTTAGAAAGATTACGAACACGACGCCGTTTAAAACAGGCGTGAGCTTCGCATCTGGAGGAGCGGGAATTTTCAACGAAACAAACAATCTTTTTGTAAGTATGTGTGTATATAAATATATGTAATTTCTTCTCCAATTTTAATTTATTTTCCATTTAAAATATGAAGAAAACAAATTTGATTTCTTTTTTTTTCACATGAATTTTTTCAGAAGCAATCAGTGGCCATGGGCCAACAAATTGACTTCTATTCGTCAGTGTACCAACATCTAGTCGAAGAGCTTGGATCCTCTGCGGCACAAGTACATCTTTCGAAATCTCTCTTCACCGTTGTGATTGGAAGTAATGACATCTTCGGCTATCATGAGTCGTCCGATCTGCGCAAAAAGTACTCCCCGCAGCAATATGTAGATTTGATGGCCTCCACCCTCGAGTCGCAACTTAAGGTATAAGCTTTTTGATAAATTTGAATGGCTTTGGTTTCACACCTATTTTAATAATAAATAATTATTTTGACAAAAATAAAATAAGATTACTTAGTCGATGGGATGAGATTTGAATGGTCCTCATTTTTCTATGAAAGGAGAGATATCTAATAATTTCATATTTAAAAGTGTGGCAAAACTGATTTAAATTTGAATAGGTGATGATTTTATTTTTTATTATATTTATATAAGAAATAGTATTTTTTTTTTGCGATATTTATATAAATAGTATCAAATGAATAAATGTTCTCAAATATAACTCATAGTTTGAAATCTTATAAAAGTAGCATAATTATAAAAAAAACTAAAATGTATGGTAAAATACACTTGGGAGAGTACCGTATATTTTGAAAAGGAAAAAAATAGTGACAATTTTAGAAATACACATAAAATTTATGCTATGTAATGAAAAATTTCTCTCTTTCCTTACCTTTTTAAAACAACTATCTAATTTCGAAAGTGAAGATCAAGTTTTAACCTACTTTATGAGGAAAATTACATCAACATGTCATAATTTCAAATTTACTTTATAGAAAAATAATTTGTAAATATGACCAATTTTTCTCTCGTACACTTTATATATCTAATATAACTTGGAGCATCGATTTACTTTTCCTTCTCTTTTTTTGTTCTTCAAGTTTTAAAATTGGACTAAAATGAGAAAGAAAAAGGAGTTAGGAAAAACGTAAAAGAATTGTTTAACAAATGAGATTATTTATGTTGTTTAAATACTTATTTAGCTTTTAAATTTTTATGTTTATGGTATTTTGACTTCTAAACTTTCAAAATCTCTATTTTAGTCCTTTCACTTTTAAAATATAGTTAATAGTTAATTTGGTCCACACAGTAATTTTGGCATAAGTTATTTAATAGATGAACCACTTTAAATATGTATACTATTTAGTTGATTTGCATATTTATTAGAATCATATCAGCACATTAATATACATATTTGAAGTTAAACATCCTTCGTTAAACAATTGACAGCATAAATGATCACAATGATTTAAAATAGAAACAGTTTAAAAGTTCAAGACCAAAATGAAATTTAAATTATTATTTTTTGAAATGAAATAGAAAACAAAACCCGAATTTCATTGTTGGTTTTCTGTTTTAGAAATAATTTAGGCTTGATTTCTCACCATTGCTTCAGTTTGGTTTTCATGCTCTTGAAAACACATTTGAGTCCTCAGCTAAATTTTCAAACATAAATATATTTAAGATAATAAACTACCACTCAAAAAGTGGAAATCATAATAAATTGAGAGGAAACAACAATACACGGGTTCGAACGCAGGACCTCTCGATGTGATGACATGTTAAGATGAATAAATCACCCTTCAATCCAAAAACTTAAGCTATTGAGTTAGTTAAATTTAATTAATTTATGAGTATATGGACAAAATAAAAAAGGAAATTGTTATCCAATCGAAATTGAAAGCTCAGATATTGTTGAACTGCAGCGATTATATAGCTATGGAGCAAGAAAATATGTGATAGGCGGGGTTGGCTTAGTGGGGTGCACTCCATCGCAAAGGAAGAGGAGCCGAACAGGCGAATGCGATGCGGAAGTCAATTCGTGGGTGGAGCACTACAACTTTGGCCTAAAAGCAATGCTTGGGAGACTCAAAATGGAGCTCAACGGCATTGCATTCTCCTACTTCGATGCATATGCAGTCATGTCCAACTTCATTCGCACACCATCCTCTTACGGTAACCCCATTATTATTTTTTTTCTTTTTATTCAATATTTCCATCTTTTAGGTAAGTAGTGTTTTGACTGAATGAGTTATGCACGTGTAAGCTACCCAAAATAGAGTTTAGAATTTATAATGATCTTAAACTGTACTTGTATCTACAATGAAAAGATCAAAAATCTAGAGGTGATACATGAGTTACTCCTCCTCTTACTAATTGATTTTGAGATGAAACTCATTAATATGGGTATAGAGTCCAAAAAAGCATTTGGTAAAAAAAAAAATCTGAATCTGAATCTCACACATTATATAAGATACAATGAGTTATATATGGGTATCAGAACCCATGAATGTTTGATAATATTGGTCATGGGTTGATTTTTTAGTTGGTGTAAGGGTTTTTTTTTCCCTTGAGGTACCCTATTCTTCAAGTGGAAATTATTCCAACTCAAACACACCAAAGAAAATAGTAGTCAAGATTAGTGGGATGAGGAGATGTTTGAACACTCGGCCGCTAGCTTATTAATGTTCATGGAATTCTATCTCAAAATCAATTGGCAATAAGAGGAGTAGTTTAGGACTATTATAAAACTTTATAAGATCTTATTTTCTTTTCAATATGAGATCTTTAACATGCTCCCTCAAGATCGTGGCTTTATTGGCTCATTAATCTTGGTTGGATCTCATTTTTTTGACCGGATGCTCGTTTGTGCGTGTTGGACTCTTATACCATATTAATATTCATGATATTTCATCTCAAAATCCATTAACAACAACAGAAGTATCTAAGCTATAAGGTCCATTTTCCTTTTAACATGGATCCTTTATTCTAACTGTTAGAAGAATATGGTTGGATGTTGTTTGTGATTTCAGGATTCACAGAGATTAGAAGGGCTTGTTGTGGGCTTGGAAACCTAAATGCCGATGTTCCATGTTTACCAATTGCAGCATTTTGCTCAAATAGAAACGACCATCTCTTTTGGGATCTCTATCATCCTACCCAAGAAGCCCATCGCCTCTTTGGCAATTACATATTTGATGGTCCATTTACGTACGCTCTCAATGTCAAGCAACTCATTGCTCTTTAAACCACCAAAAAAGAAAAACAAAAAAAAGTGCCATCACACACACATTATTATCTAATTCTTGTATTGCTGTTAGAGTCCACAAGGAAAGAAAGTGAGTAATAAATGTATACTATATCTTTTGATATAGATGAAGAGAGTTCAAAAAAGATTCGTCTAGTAATATTCTCGCATTGCTGACT

mRNA sequence

CTAGACTCTAATAAGAAGAGAAGTTATGGAAAGAAAATGGGAACAGACAAATAATGGACAACTTGGTCCAGAAGAGAATGATTATTAAATATGAATAGAAATTCCCCACACATATCCACATGGGTCCCCACCCAACCCATCAACATACTCCTCTTCTTCTCTTACTTTTTCTAATTTTCACACTCAATTTTGGATTCTCATCTCTCTCTCTCTCTCTCTATATAAATTCCCTTTCAAGTTTCAATATTCCATTGAAGAAAAAGGAAATGGGTTCCAGAGCCTTCTTCTTCTTCTTCATCTTCTTGTGTGTATCATTCAGTTTTAGAGTTTGTGAATGTGGTGCCTTAGTTCCTGCAATCTATGTCTTCGGAGACTCCTTGGTTGATGTCGGCAACAACAACCACTTGAAGCTCTCTCTCGCCAAAGCCAACTTCCCTCACAATGGAATCGACTTCCCCACTACTAAACCCACCGGAAGGTTCTCTAATGGCAAGAACGCTGCCGACTATCTTGCTGAGAAAGTAGGCCTACCGACTTCACCACCGTATCTCTCCCTAGTATCCAAGTTTAGAAAGATTACGAACACGACGCCGTTTAAAACAGGCGTGAGCTTCGCATCTGGAGGAGCGGGAATTTTCAACGAAACAAACAATCTTTTTGTAAGTATGTTGGCCATGGGCCAACAAATTGACTTCTATTCGTCAGTGTACCAACATCTAGTCGAAGAGCTTGGATCCTCTGCGGCACAAGTACATCTTTCGAAATCTCTCTTCACCGTTGTGATTGGAAGTAATGACATCTTCGGCTATCATGAGTCGTCCGATCTGCGCAAAAAGTACTCCCCGCAGCAATATGTAGATTTGATGGCCTCCACCCTCGAGTCGCAACTTAAGCGATTATATAGCTATGGAGCAAGAAAATATGTGATAGGCGGGGTTGGCTTAGTGGGGTGCACTCCATCGCAAAGGAAGAGGAGCCGAACAGGCGAATGCGATGCGGAAGTCAATTCGTGGGTGGAGCACTACAACTTTGGCCTAAAAGCAATGCTTGGGAGACTCAAAATGGAGCTCAACGGCATTGCATTCTCCTACTTCGATGCATATGCAGTCATGTCCAACTTCATTCGCACACCATCCTCTTACGGATTCACAGAGATTAGAAGGGCTTGTTGTGGGCTTGGAAACCTAAATGCCGATGTTCCATGTTTACCAATTGCAGCATTTTGCTCAAATAGAAACGACCATCTCTTTTGGGATCTCTATCATCCTACCCAAGAAGCCCATCGCCTCTTTGGCAATTACATATTTGATGGTCCATTTACGTACGCTCTCAATGTCAAGCAACTCATTGCTCTTTAAACCACCAAAAAAGAAAAACAAAAAAAAGTGCCATCACACACACATTATTATCTAATTCTTGTATTGCTGTTAGAGTCCACAAGGAAAGAAAGTGAGTAATAAATGTATACTATATCTTTTGATATAGATGAAGAGAGTTCAAAAAAGATTCGTCTAGTAATATTCTCGCATTGCTGACT

Coding sequence (CDS)

ATGGGTCCCCACCCAACCCATCAACATACTCCTCTTCTTCTCTTACTTTTTCTAATTTTCACACTCAATTTTGGATTCTCATCTCTCTCTCTCTCTCTCTATATAAATTCCCTTTCAAGTTTCAATATTCCATTGAAGAAAAAGGAAATGGGTTCCAGAGCCTTCTTCTTCTTCTTCATCTTCTTGTGTGTATCATTCAGTTTTAGAGTTTGTGAATGTGGTGCCTTAGTTCCTGCAATCTATGTCTTCGGAGACTCCTTGGTTGATGTCGGCAACAACAACCACTTGAAGCTCTCTCTCGCCAAAGCCAACTTCCCTCACAATGGAATCGACTTCCCCACTACTAAACCCACCGGAAGGTTCTCTAATGGCAAGAACGCTGCCGACTATCTTGCTGAGAAAGTAGGCCTACCGACTTCACCACCGTATCTCTCCCTAGTATCCAAGTTTAGAAAGATTACGAACACGACGCCGTTTAAAACAGGCGTGAGCTTCGCATCTGGAGGAGCGGGAATTTTCAACGAAACAAACAATCTTTTTGTAAGTATGTTGGCCATGGGCCAACAAATTGACTTCTATTCGTCAGTGTACCAACATCTAGTCGAAGAGCTTGGATCCTCTGCGGCACAAGTACATCTTTCGAAATCTCTCTTCACCGTTGTGATTGGAAGTAATGACATCTTCGGCTATCATGAGTCGTCCGATCTGCGCAAAAAGTACTCCCCGCAGCAATATGTAGATTTGATGGCCTCCACCCTCGAGTCGCAACTTAAGCGATTATATAGCTATGGAGCAAGAAAATATGTGATAGGCGGGGTTGGCTTAGTGGGGTGCACTCCATCGCAAAGGAAGAGGAGCCGAACAGGCGAATGCGATGCGGAAGTCAATTCGTGGGTGGAGCACTACAACTTTGGCCTAAAAGCAATGCTTGGGAGACTCAAAATGGAGCTCAACGGCATTGCATTCTCCTACTTCGATGCATATGCAGTCATGTCCAACTTCATTCGCACACCATCCTCTTACGGATTCACAGAGATTAGAAGGGCTTGTTGTGGGCTTGGAAACCTAAATGCCGATGTTCCATGTTTACCAATTGCAGCATTTTGCTCAAATAGAAACGACCATCTCTTTTGGGATCTCTATCATCCTACCCAAGAAGCCCATCGCCTCTTTGGCAATTACATATTTGATGGTCCATTTACGTACGCTCTCAATGTCAAGCAACTCATTGCTCTTTAA

Protein sequence

MGPHPTHQHTPLLLLLFLIFTLNFGFSSLSLSLYINSLSSFNIPLKKKEMGSRAFFFFFIFLCVSFSFRVCECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNGIDFPTTKPTGRFSNGKNAADYLAEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGGAGIFNETNNLFVSMLAMGQQIDFYSSVYQHLVEELGSSAAQVHLSKSLFTVVIGSNDIFGYHESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQRKRSRTGECDAEVNSWVEHYNFGLKAMLGRLKMELNGIAFSYFDAYAVMSNFIRTPSSYGFTEIRRACCGLGNLNADVPCLPIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFDGPFTYALNVKQLIAL
Homology
BLAST of MC10g1066 vs. ExPASy Swiss-Prot
Match: Q9FIA1 (GDSL esterase/lipase At5g55050 OS=Arabidopsis thaliana OX=3702 GN=At5g55050 PE=2 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.7e-94
Identity = 174/342 (50.88%), Postives = 232/342 (67.84%), Query Frame = 0

Query: 75  ALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNGIDFPTTKPTGRFSNGKNAADYLAEK 134
           A +P +YVFGDSLVD GNNN+L +S++KAN+PHNG+DFP  KPTGRF NGKNAAD +AEK
Sbjct: 35  ASIPGLYVFGDSLVDAGNNNYLPISISKANYPHNGVDFPNKKPTGRFCNGKNAADAIAEK 94

Query: 135 VGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGGAGIFNETNNLFVSMLAMGQQIDFYS 194
            GLP  PPYLSL    ++    +   TGV+FASGGAGIFN ++      + + +Q++ + 
Sbjct: 95  FGLPLPPPYLSLRGLLKREKRKSAAVTGVNFASGGAGIFNSSDQKLGQAIPLSKQVNNWL 154

Query: 195 SVYQHLVEELGSSAAQVHLSKSLFTVVIGSNDIFGYHESSDLRKKYSPQQYVDLMASTLE 254
           S+++  V +L  SAAQ+HLSKSLFTVVIGSND+F Y  S  LR++ +PQQY  LMA  L+
Sbjct: 155 SIHEE-VMKLEPSAAQLHLSKSLFTVVIGSNDLFDYFGSFKLRRQSNPQQYTQLMADKLK 214

Query: 255 SQLKRLYSYGARKYVIGGVGLVGCTPSQR-KRSRTGECDAEVNSWVEHYNFGLKAMLGRL 314
            QLKR++  GAR+++I GV  +GCTP +R K S   ECD   N W   YN  L  ML +L
Sbjct: 215 EQLKRIHDSGARRFLIIGVAQIGCTPGKRAKNSTLHECDEGANMWCSLYNEALVKMLQQL 274

Query: 315 KMELNG-IAFSYFDAYAVMSNFIRTPSSYGFTEIRRACCGLGNLNADVPCLPIAAFCSNR 374
           K EL G I ++YFD Y  + + I  P+ YGF ++  ACCG G LNAD+PCLP+A  CS+R
Sbjct: 275 KQELQGSITYTYFDNYKSLHDIISNPARYGFADVTSACCGNGELNADLPCLPLAKLCSDR 334

Query: 375 NDHLFWDLY-HPTQEAHRLFGNYIF--DGPFTYALNVKQLIA 412
             HLFWD Y HPT+ A R   + +   D  ++  + + QL++
Sbjct: 335 TKHLFWDRYGHPTEAAARTIVDLMLTDDTHYSSPITLTQLVS 375

BLAST of MC10g1066 vs. ExPASy Swiss-Prot
Match: Q9FHQ1 (GDSL esterase/lipase At5g37690 OS=Arabidopsis thaliana OX=3702 GN=At5g37690 PE=2 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 4.0e-64
Identity = 121/318 (38.05%), Postives = 188/318 (59.12%), Query Frame = 0

Query: 81  YVFGDSLVDVGNNNHLKLSLAKANFPHNGIDFPTTKPTGRFSNGKNAADYLAEKVGLPTS 140
           Y+FGDSL +VGNNN L+ SLA+A+FP+ G+DF   K TGRF+NG+   D ++ K+G+ + 
Sbjct: 29  YIFGDSLTEVGNNNFLQYSLARADFPYYGVDFSGGKATGRFTNGRTIGDIISTKLGILSP 88

Query: 141 PPYLSLVSKFRKITNTTPFKTGVSFASGGAGIFNETNNLFVSMLAMGQQIDFYSSVYQHL 200
           PPYLSL        N   F +G+++ASGGAGI NET   F+  L    QI+ +    + +
Sbjct: 89  PPYLSLSQ------NDDAFLSGINYASGGAGILNETGIYFIQRLTFNDQINCFKKTKEVI 148

Query: 201 VEELGSSAAQVHLSKSLFTVVIGSNDIFGYHESSDLR--KKYSPQQYVDLMASTLESQLK 260
             ++G  AA  H++ +++ + +GSND         +   ++Y+  ++V+L+ STL +QL 
Sbjct: 149 RAKIGDGAANKHVNDAMYFIGLGSNDYVNNFLQPFMADGQQYTHDEFVELLTSTLHNQLT 208

Query: 261 RLYSYGARKYVIGGVGLVGCTPSQRKRSRTGECDAEVNSWVEHYNFGLKAMLGRLKMELN 320
            +Y  GARK +  G+G +GC PSQR +S+T  C   VN WV  +N   K +L  L   L 
Sbjct: 209 TIYKLGARKVIFHGLGPLGCIPSQRVKSKTRMCLNRVNEWVLEFNSRTKKLLIDLNKRLP 268

Query: 321 GIAFSYFDAYAVMSNFIRTPSSYGFTEIRRACCGLGNLNADVPCLPIAAFCSNRNDHLFW 380
           G  FS+ D Y  + + I  P+ YGF     +CC + + +    CLP +  C NR D +FW
Sbjct: 269 GAKFSFADTYPAVLDLINNPTHYGFKIANTSCCNV-DTSVGGLCLPNSKMCKNRQDFVFW 328

Query: 381 DLYHPTQEAHRLFGNYIF 397
           D +HP+  A+++  +++F
Sbjct: 329 DAFHPSDSANQILADHLF 339

BLAST of MC10g1066 vs. ExPASy Swiss-Prot
Match: O23470 (GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3 SV=2)

HSP 1 Score: 233.8 bits (595), Expect = 3.5e-60
Identity = 138/373 (37.00%), Postives = 204/373 (54.69%), Query Frame = 0

Query: 50  MGSRAFFFFFIFLCVSFSFRVCECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNG 109
           M    F    I L V F   VC  G  +PA +VFGDSLVD GNNN+L  +L+KAN+  NG
Sbjct: 1   MSLLVFLCQIIVLSVLFFSEVCLAGKKIPANFVFGDSLVDAGNNNYL-ATLSKANYVPNG 60

Query: 110 IDFPTTKPTGRFSNGKNAADYLAEKVGL-PTSPPYLSLVSKFRKITNTTPFKTGVSFASG 169
           IDF +  PTGRF+NG+   D + + +G    +PPYL+  +    I N      GV++ASG
Sbjct: 61  IDFGS--PTGRFTNGRTIVDIVYQALGSDELTPPYLAPTTSGSLILN------GVNYASG 120

Query: 170 GAGIFNETNNLFVSMLAMGQQIDFYSSVYQHLVEELGSSAAQVHLSKSLFTVVIGSNDIF 229
           G+GI N T  LF   + +  Q+D +++  Q ++  +G S A      ++F+V  GSND+ 
Sbjct: 121 GSGILNSTGKLFGERINVDAQLDNFATTRQDIISWIGESEAAKLFRSAIFSVTTGSNDLI 180

Query: 230 GYH----ESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQRK 289
             +     S+  RK  +P+ +VD M S    QL RLY  GARK V+  +G +GC P +R+
Sbjct: 181 NNYFTPVISTLQRKVVAPEVFVDTMISKFRLQLTRLYQLGARKIVVINIGPIGCIPFERE 240

Query: 290 R--SRTGECDAEVNSWVEHYNFGLKAMLGRLKMELNGIAFSYFDAYAVMSNFIRTPSSYG 349
              +    C AE N   + YN  LK ++  L   L G  F Y D + ++ + I+  SSYG
Sbjct: 241 SDPAAGNNCLAEPNEVAQMYNLKLKTLVEELNKNLQGSRFVYGDVFRIVDDIIQNYSSYG 300

Query: 350 FTEIRRACCGL-GNLNADVPCLPIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFDGPFT 409
           F   +  CC L G +   +PC P +  C +R+ ++FWD YHPT+ A+ +    +  G  +
Sbjct: 301 FESEKIPCCSLVGKVGGLIPCGPPSKVCMDRSKYVFWDPYHPTEAANIIIARRLLSGDTS 360

Query: 410 --YALNVKQLIAL 413
             Y +N++QL  L
Sbjct: 361 DIYPINIRQLANL 364

BLAST of MC10g1066 vs. ExPASy Swiss-Prot
Match: Q9FNP2 (GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana OX=3702 GN=At5g08460 PE=2 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.6e-57
Identity = 125/351 (35.61%), Postives = 186/351 (52.99%), Query Frame = 0

Query: 72  ECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNGIDFPTTKPTGRFSNGKNAADYL 131
           E  A+ PA++VFGDSLVD GNNNHL  SLA++N+   GIDF   +PTGRFSNGK   D++
Sbjct: 42  ETTAMFPAMFVFGDSLVDNGNNNHLN-SLARSNYLPYGIDFAGNQPTGRFSNGKTIVDFI 101

Query: 132 AEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGGAGIFNETNNLFVSMLAMGQQID 191
            E +GLP  P ++  V     I +      GV++AS   GI  ET        +MG+Q++
Sbjct: 102 GELLGLPEIPAFMDTVDGGVDILH------GVNYASAAGGILEETGRHLGERFSMGRQVE 161

Query: 192 FYSSVYQHLVEELGSSAAQVHLSKSLFTVVIGSNDIFGYHESSDL---RKKYSPQQYVDL 251
            +      +   +   + + +++KSL  V +G+ND    +    L      Y P  + DL
Sbjct: 162 NFEKTLMEISRSMRKESVKEYMAKSLVVVSLGNNDYINNYLKPRLFLSSSIYDPTSFADL 221

Query: 252 MASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQ--RKRSRTGECDAEVNSWVEHYNFGL 311
           + S   + L  LY  G RK+VI GVG +GC P Q   + +  GEC   VN   E +N  L
Sbjct: 222 LLSNFTTHLLELYGKGFRKFVIAGVGPLGCIPDQLAAQAALPGECVEAVNEMAELFNNRL 281

Query: 312 KAMLGRLKME---LNGIAFSYFDAYAVMSNFIRTPSSYGFTEIRRACCGLGNLNADVPCL 371
            +++ RL  +    +   F Y + Y    + +  P +YGF    R CCG+G    ++ CL
Sbjct: 282 VSLVDRLNSDNKTASEAIFVYGNTYGAAVDILTNPFNYGFEVTDRGCCGVGRNRGEITCL 341

Query: 372 PIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFDGPFT--YALNVKQLIAL 413
           P+A  C+ R+ H+FWD +HPTQ  + +     F+G  +  Y +N+ QL  L
Sbjct: 342 PLAVPCAFRDRHVFWDAFHPTQAFNLIIALRAFNGSKSDCYPINLSQLSRL 385

BLAST of MC10g1066 vs. ExPASy Swiss-Prot
Match: Q9SF78 (GDSL esterase/lipase At1g71691 OS=Arabidopsis thaliana OX=3702 GN=At1g71691 PE=2 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 6.2e-57
Identity = 121/342 (35.38%), Postives = 182/342 (53.22%), Query Frame = 0

Query: 76  LVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNGIDFPTTKPTGRFSNGKNAADYLAEKV 135
           +VPA++VFGDSL+D GNNN++  S AKAN+   GIDF    PTGRF NG    D +A+ +
Sbjct: 52  IVPALFVFGDSLIDNGNNNNIP-SFAKANYFPYGIDF-NGGPTGRFCNGLTMVDGIAQLL 111

Query: 136 GLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGGAGIFNETNNLFVSMLAMGQQIDFYSS 195
           GLP  P Y                  GV++AS  AGI  +T   FV  +   QQI  + +
Sbjct: 112 GLPLIPAYSEATG--------DQVLRGVNYASAAAGILPDTGGNFVGRIPFDQQIHNFET 171

Query: 196 VYQHLVEELGSSAAQV-HLSKSLFTVVIGSNDIFGYH--ESSDLRKKYSPQQYVDLMAST 255
               +  + G + A    +++SLF + +GSND    +   +   R +Y+ QQ+ DL+   
Sbjct: 172 TLDQVASKSGGAVAIADSVTRSLFFIGMGSNDYLNNYLMPNFPTRNQYNSQQFGDLLVQH 231

Query: 256 LESQLKRLYSYGARKYVIGGVGLVGCTPSQRKRSRTGECDAEVNSWVEHYNFGLKAMLGR 315
              QL RLY+ G RK+V+ G+G +GC PS   +   G+C  EVN  V  +N  +K M+  
Sbjct: 232 YTDQLTRLYNLGGRKFVVAGLGRMGCIPSILAQGNDGKCSEEVNQLVLPFNTNVKTMISN 291

Query: 316 LKMELNGIAFSYFDAYAVMSNFIRTPSSYGFTEIRRACCGLGNLNADVPCLPIAAFCSNR 375
           L   L    F Y D   +  + +   ++YG T + + CCG+G     + CLP    C NR
Sbjct: 292 LNQNLPDAKFIYLDIAHMFEDIVANQAAYGLTTMDKGCCGIGKNRGQITCLPFETPCPNR 351

Query: 376 NDHLFWDLYHPTQEAHRLFGNYIFDGPFT--YALNVKQLIAL 413
           + ++FWD +HPT++ + +     F G  T  Y +N++QL +L
Sbjct: 352 DQYVFWDAFHPTEKVNLIMAKKAFAGDRTVAYPINIQQLASL 383

BLAST of MC10g1066 vs. NCBI nr
Match: XP_022151286.1 (GDSL esterase/lipase At5g55050-like [Momordica charantia])

HSP 1 Score: 734 bits (1895), Expect = 1.88e-266
Identity = 359/363 (98.90%), Postives = 360/363 (99.17%), Query Frame = 0

Query: 50  MGSRAFFFFFIFLCVSFSFRVCECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNG 109
           MGSRAFFFFFIFLCVSFSFRVCECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNG
Sbjct: 1   MGSRAFFFFFIFLCVSFSFRVCECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNG 60

Query: 110 IDFPTTKPTGRFSNGKNAADYLAEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGG 169
           IDFPTTKPTGRFSNGKNAADYLAEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGG
Sbjct: 61  IDFPTTKPTGRFSNGKNAADYLAEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGG 120

Query: 170 AGIFNETNNLFVSMLAMGQQIDFYSSVYQHLVEELGSSAAQVHLSKSLFTVVIGSNDIFG 229
           AGIFNETNNLF   +AMGQQIDFYSSVYQHLVEELGSSAAQVHLSKSLFTVVIGSNDIFG
Sbjct: 121 AGIFNETNNLFKQSVAMGQQIDFYSSVYQHLVEELGSSAAQVHLSKSLFTVVIGSNDIFG 180

Query: 230 YHESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQRKRSRTG 289
           YHESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQRKRSRTG
Sbjct: 181 YHESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQRKRSRTG 240

Query: 290 ECDAEVNSWVEHYNFGLKAMLGRLKMELNGIAFSYFDAYAVMSNFIRTPSSYGFTEIRRA 349
           ECDAEVNSWVEHYNFGLKAMLGRLKMELNGIAFSYFDAYAVMSNFIRTPSSYGFTEIRRA
Sbjct: 241 ECDAEVNSWVEHYNFGLKAMLGRLKMELNGIAFSYFDAYAVMSNFIRTPSSYGFTEIRRA 300

Query: 350 CCGLGNLNADVPCLPIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFDGPFTYALNVKQL 409
           CCGLGNLNADVPCLPIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFDGPFTYALNVKQL
Sbjct: 301 CCGLGNLNADVPCLPIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFDGPFTYALNVKQL 360

Query: 410 IAL 412
           IAL
Sbjct: 361 IAL 363

BLAST of MC10g1066 vs. NCBI nr
Match: XP_008449153.1 (PREDICTED: GDSL esterase/lipase At5g55050 [Cucumis melo])

HSP 1 Score: 588 bits (1515), Expect = 1.53e-208
Identity = 287/366 (78.42%), Postives = 314/366 (85.79%), Query Frame = 0

Query: 50  MGSR---AFFFFFIFLCVSFSFRVCECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFP 109
           MGS+   +F FF  F   S    V    +LVPAIYVFGDSLVDVGNNNHLKLSLAKANFP
Sbjct: 1   MGSQVLLSFLFFLFFFFFSILMGVSN-SSLVPAIYVFGDSLVDVGNNNHLKLSLAKANFP 60

Query: 110 HNGIDFPTTKPTGRFSNGKNAADYLAEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFA 169
           HNG+DFPT KPTGRFSNGKNAAD++AE+VGL TSPPYLSL+SKFRK  NTTPFKTGVSFA
Sbjct: 61  HNGLDFPTKKPTGRFSNGKNAADFVAERVGLATSPPYLSLISKFRKTVNTTPFKTGVSFA 120

Query: 170 SGGAGIFNETNNLFVSMLAMGQQIDFYSSVYQHLVEELGSSAAQVHLSKSLFTVVIGSND 229
           SGGAGIFNETNNLF   +AM QQI+ YS VY+HLV ELGSS A  HLSKSLFTVVIGSND
Sbjct: 121 SGGAGIFNETNNLFKQSVAMDQQIELYSRVYKHLVGELGSSGAAAHLSKSLFTVVIGSND 180

Query: 230 IFGYHESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQRKRS 289
           IFGYHESSDLR+KYSPQQY+DLMASTL SQLKRL+ YGARKYV+GG+GLVGC PSQRKRS
Sbjct: 181 IFGYHESSDLRQKYSPQQYLDLMASTLHSQLKRLHGYGARKYVVGGIGLVGCAPSQRKRS 240

Query: 290 RTGECDAEVNSWVEHYNFGLKAMLGRLKMELNGIAFSYFDAYAVMSNFIRTPSSYGFTEI 349
           +TGECD EVN+W   YN  LK+ L  LKMELN I+FS+FD Y VMSNF+ +PSSYGFTEI
Sbjct: 241 KTGECDEEVNNWAAIYNTALKSKLETLKMELNDISFSFFDVYQVMSNFVHSPSSYGFTEI 300

Query: 350 RRACCGLGNLNADVPCLPIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFDGPFTYALNV 409
           + ACCGLGNLNADVPCLPIA FCSNRN+HLFWDLYHPTQEAHR+F NYIFDGPFTY LN+
Sbjct: 301 KSACCGLGNLNADVPCLPIAKFCSNRNNHLFWDLYHPTQEAHRMFANYIFDGPFTYPLNL 360

Query: 410 KQLIAL 412
            QLIAL
Sbjct: 361 NQLIAL 365

BLAST of MC10g1066 vs. NCBI nr
Match: XP_022932219.1 (GDSL esterase/lipase At5g55050 [Cucurbita moschata])

HSP 1 Score: 584 bits (1505), Expect = 4.55e-207
Identity = 289/364 (79.40%), Postives = 317/364 (87.09%), Query Frame = 0

Query: 50  MGSRAFFFFFIFLCVSFSFRVCECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNG 109
           MGS+  FFF   L   FS  + E  ALVPAIYVFGDSLVDVGNNNHLKLS+AKANFPHNG
Sbjct: 1   MGSQILFFFMFSLLSIFS-TISE-SALVPAIYVFGDSLVDVGNNNHLKLSIAKANFPHNG 60

Query: 110 IDFPTTKPTGRFSNGKNAADYLAEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGG 169
           IDFPT KPTGRFSNGKNAADY+AEKVGLPTSPPYLSL+SKFRK+ NT PFKTGVSFASGG
Sbjct: 61  IDFPTKKPTGRFSNGKNAADYIAEKVGLPTSPPYLSLISKFRKVINTKPFKTGVSFASGG 120

Query: 170 AGIFNETNNLFVSMLAMGQQIDFYSSVYQHLVEELGSSAAQVHLSKSLFTVVIGSNDIFG 229
           AGIFNETNNLF   +AM QQI++YS VYQ LV ELG SAAQ HLSKSLFTVVIGSNDIFG
Sbjct: 121 AGIFNETNNLFKQSVAMDQQIEYYSLVYQKLVVELGLSAAQAHLSKSLFTVVIGSNDIFG 180

Query: 230 YHESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQRKRSRTG 289
           YHESSDLRKKYSPQQYV+ MASTL+SQLKRLY YGARKYV+ G+GLVGCTPSQRKRS+TG
Sbjct: 181 YHESSDLRKKYSPQQYVESMASTLKSQLKRLYGYGARKYVVTGIGLVGCTPSQRKRSKTG 240

Query: 290 ECDAEVNSWVEHYNFGLKAMLGRLKMELNG-IAFSYFDAYAVMSNFIRTPSSYGFTEIRR 349
           ECD EVNSW   YN  LK+ML  LKMELN  I+F+YF+ YAVMS+FIR+PSS+GFTEI+ 
Sbjct: 241 ECDEEVNSWAAEYNVALKSMLQTLKMELNNNISFTYFEVYAVMSDFIRSPSSFGFTEIKS 300

Query: 350 ACCGLGNLNADVPCLPIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFDGPFTYALNVKQ 409
           ACCG G+LNAD+PCLPIAAFCSNRN HLFWDLYHPTQEA R+  +YIF+GPFTY +NV Q
Sbjct: 301 ACCGHGHLNADLPCLPIAAFCSNRNSHLFWDLYHPTQEASRVLADYIFNGPFTYPINVNQ 360

Query: 410 LIAL 412
           LIAL
Sbjct: 361 LIAL 362

BLAST of MC10g1066 vs. NCBI nr
Match: XP_022965085.1 (GDSL esterase/lipase At5g55050 [Cucurbita maxima])

HSP 1 Score: 583 bits (1503), Expect = 9.18e-207
Identity = 287/364 (78.85%), Postives = 318/364 (87.36%), Query Frame = 0

Query: 50  MGSRAFFFFFIFLCVSFSFRVCECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNG 109
           MGS+  F F IF  +S    + E  ALVPAIYVFGDSLVDVGNNNHLKLS+AKANFPHNG
Sbjct: 1   MGSQLLFCF-IFSLLSIFSTISE-SALVPAIYVFGDSLVDVGNNNHLKLSIAKANFPHNG 60

Query: 110 IDFPTTKPTGRFSNGKNAADYLAEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGG 169
           IDFPT KPTGRFSNGKNAADY+AEKVGLPTSPPYLSL+SKFRK+ NT PFKTGVSFASGG
Sbjct: 61  IDFPTKKPTGRFSNGKNAADYIAEKVGLPTSPPYLSLISKFRKVINTKPFKTGVSFASGG 120

Query: 170 AGIFNETNNLFVSMLAMGQQIDFYSSVYQHLVEELGSSAAQVHLSKSLFTVVIGSNDIFG 229
           AGIFNETNNLF   +A+ QQI++YS VYQ+LV ELG SAA+ HLSKSLFTVVIGSNDIFG
Sbjct: 121 AGIFNETNNLFKQSVAIDQQIEYYSLVYQNLVVELGLSAAEAHLSKSLFTVVIGSNDIFG 180

Query: 230 YHESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQRKRSRTG 289
           YHESSDLRKKYSPQQYV+ MASTL+SQLKRLY YGARKYV+ G+GLVGCTPSQRKRS+TG
Sbjct: 181 YHESSDLRKKYSPQQYVESMASTLKSQLKRLYGYGARKYVVTGIGLVGCTPSQRKRSKTG 240

Query: 290 ECDAEVNSWVEHYNFGLKAMLGRLKMELNG-IAFSYFDAYAVMSNFIRTPSSYGFTEIRR 349
           ECD EVNSW   YN  LK+ML  LKMELN  I+FSYF+ YA+MS+F+R+PSSYGFTEIR 
Sbjct: 241 ECDVEVNSWAAQYNVALKSMLQTLKMELNNNISFSYFEVYAIMSDFVRSPSSYGFTEIRS 300

Query: 350 ACCGLGNLNADVPCLPIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFDGPFTYALNVKQ 409
           ACCG G+LNAD+PCLPIAAFCSNRN HLFWDLYHPTQEA R+  +YIF+GPFTY +NV Q
Sbjct: 301 ACCGHGHLNADLPCLPIAAFCSNRNSHLFWDLYHPTQEASRMLADYIFNGPFTYPINVNQ 360

Query: 410 LIAL 412
           LIAL
Sbjct: 361 LIAL 362

BLAST of MC10g1066 vs. NCBI nr
Match: XP_023552078.1 (GDSL esterase/lipase At5g55050 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 582 bits (1501), Expect = 1.78e-206
Identity = 289/364 (79.40%), Postives = 319/364 (87.64%), Query Frame = 0

Query: 50  MGSRAFFFFFIFLCVSFSFRVCECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNG 109
           MGS+  FFF IF  +S S  + E  ALVPA+YVFGDSLVDVGNNNHLKLS+AKANFPHNG
Sbjct: 1   MGSQLLFFF-IFSLLSIS-SISE-SALVPAVYVFGDSLVDVGNNNHLKLSIAKANFPHNG 60

Query: 110 IDFPTTKPTGRFSNGKNAADYLAEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGG 169
           IDFPT KPTGRFSNGKNAADY+AEKVGLPTSPPYLSL+SKFRK+ NT PFKTGVSFASGG
Sbjct: 61  IDFPTKKPTGRFSNGKNAADYIAEKVGLPTSPPYLSLISKFRKVINTKPFKTGVSFASGG 120

Query: 170 AGIFNETNNLFVSMLAMGQQIDFYSSVYQHLVEELGSSAAQVHLSKSLFTVVIGSNDIFG 229
           AGIFNETN+LF   +AM QQI++YS VYQ LV ELG SAAQ HLSKSLFTVVIGSNDIFG
Sbjct: 121 AGIFNETNDLFKQSVAMDQQIEYYSLVYQKLVVELGLSAAQAHLSKSLFTVVIGSNDIFG 180

Query: 230 YHESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQRKRSRTG 289
           YHESSDLRKKYSPQQYV+ MASTL+SQLKRLY YGARKYV+ G+GLVGCTPSQRKRS+TG
Sbjct: 181 YHESSDLRKKYSPQQYVESMASTLKSQLKRLYGYGARKYVVTGIGLVGCTPSQRKRSKTG 240

Query: 290 ECDAEVNSWVEHYNFGLKAMLGRLKMELNG-IAFSYFDAYAVMSNFIRTPSSYGFTEIRR 349
           ECD EVNSW   YN  LK+ML  LKMELN  I+F+YF+ YAVMS+FIR+PSS+GFTEIR 
Sbjct: 241 ECDEEVNSWAAQYNVALKSMLQTLKMELNNNISFTYFEVYAVMSDFIRSPSSFGFTEIRS 300

Query: 350 ACCGLGNLNADVPCLPIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFDGPFTYALNVKQ 409
           ACCG G+LNAD+PCLPIAAFCSNRN HLFWDLYHPTQEA R+  +YIF+GPFTY +NV Q
Sbjct: 301 ACCGHGHLNADLPCLPIAAFCSNRNSHLFWDLYHPTQEASRMLADYIFNGPFTYPINVNQ 360

Query: 410 LIAL 412
           LIAL
Sbjct: 361 LIAL 361

BLAST of MC10g1066 vs. ExPASy TrEMBL
Match: A0A6J1DE65 (GDSL esterase/lipase At5g55050-like OS=Momordica charantia OX=3673 GN=LOC111019250 PE=3 SV=1)

HSP 1 Score: 734 bits (1895), Expect = 9.09e-267
Identity = 359/363 (98.90%), Postives = 360/363 (99.17%), Query Frame = 0

Query: 50  MGSRAFFFFFIFLCVSFSFRVCECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNG 109
           MGSRAFFFFFIFLCVSFSFRVCECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNG
Sbjct: 1   MGSRAFFFFFIFLCVSFSFRVCECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNG 60

Query: 110 IDFPTTKPTGRFSNGKNAADYLAEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGG 169
           IDFPTTKPTGRFSNGKNAADYLAEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGG
Sbjct: 61  IDFPTTKPTGRFSNGKNAADYLAEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGG 120

Query: 170 AGIFNETNNLFVSMLAMGQQIDFYSSVYQHLVEELGSSAAQVHLSKSLFTVVIGSNDIFG 229
           AGIFNETNNLF   +AMGQQIDFYSSVYQHLVEELGSSAAQVHLSKSLFTVVIGSNDIFG
Sbjct: 121 AGIFNETNNLFKQSVAMGQQIDFYSSVYQHLVEELGSSAAQVHLSKSLFTVVIGSNDIFG 180

Query: 230 YHESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQRKRSRTG 289
           YHESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQRKRSRTG
Sbjct: 181 YHESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQRKRSRTG 240

Query: 290 ECDAEVNSWVEHYNFGLKAMLGRLKMELNGIAFSYFDAYAVMSNFIRTPSSYGFTEIRRA 349
           ECDAEVNSWVEHYNFGLKAMLGRLKMELNGIAFSYFDAYAVMSNFIRTPSSYGFTEIRRA
Sbjct: 241 ECDAEVNSWVEHYNFGLKAMLGRLKMELNGIAFSYFDAYAVMSNFIRTPSSYGFTEIRRA 300

Query: 350 CCGLGNLNADVPCLPIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFDGPFTYALNVKQL 409
           CCGLGNLNADVPCLPIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFDGPFTYALNVKQL
Sbjct: 301 CCGLGNLNADVPCLPIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFDGPFTYALNVKQL 360

Query: 410 IAL 412
           IAL
Sbjct: 361 IAL 363

BLAST of MC10g1066 vs. ExPASy TrEMBL
Match: A0A1S3BM17 (GDSL esterase/lipase At5g55050 OS=Cucumis melo OX=3656 GN=LOC103491100 PE=3 SV=1)

HSP 1 Score: 588 bits (1515), Expect = 7.40e-209
Identity = 287/366 (78.42%), Postives = 314/366 (85.79%), Query Frame = 0

Query: 50  MGSR---AFFFFFIFLCVSFSFRVCECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFP 109
           MGS+   +F FF  F   S    V    +LVPAIYVFGDSLVDVGNNNHLKLSLAKANFP
Sbjct: 1   MGSQVLLSFLFFLFFFFFSILMGVSN-SSLVPAIYVFGDSLVDVGNNNHLKLSLAKANFP 60

Query: 110 HNGIDFPTTKPTGRFSNGKNAADYLAEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFA 169
           HNG+DFPT KPTGRFSNGKNAAD++AE+VGL TSPPYLSL+SKFRK  NTTPFKTGVSFA
Sbjct: 61  HNGLDFPTKKPTGRFSNGKNAADFVAERVGLATSPPYLSLISKFRKTVNTTPFKTGVSFA 120

Query: 170 SGGAGIFNETNNLFVSMLAMGQQIDFYSSVYQHLVEELGSSAAQVHLSKSLFTVVIGSND 229
           SGGAGIFNETNNLF   +AM QQI+ YS VY+HLV ELGSS A  HLSKSLFTVVIGSND
Sbjct: 121 SGGAGIFNETNNLFKQSVAMDQQIELYSRVYKHLVGELGSSGAAAHLSKSLFTVVIGSND 180

Query: 230 IFGYHESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQRKRS 289
           IFGYHESSDLR+KYSPQQY+DLMASTL SQLKRL+ YGARKYV+GG+GLVGC PSQRKRS
Sbjct: 181 IFGYHESSDLRQKYSPQQYLDLMASTLHSQLKRLHGYGARKYVVGGIGLVGCAPSQRKRS 240

Query: 290 RTGECDAEVNSWVEHYNFGLKAMLGRLKMELNGIAFSYFDAYAVMSNFIRTPSSYGFTEI 349
           +TGECD EVN+W   YN  LK+ L  LKMELN I+FS+FD Y VMSNF+ +PSSYGFTEI
Sbjct: 241 KTGECDEEVNNWAAIYNTALKSKLETLKMELNDISFSFFDVYQVMSNFVHSPSSYGFTEI 300

Query: 350 RRACCGLGNLNADVPCLPIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFDGPFTYALNV 409
           + ACCGLGNLNADVPCLPIA FCSNRN+HLFWDLYHPTQEAHR+F NYIFDGPFTY LN+
Sbjct: 301 KSACCGLGNLNADVPCLPIAKFCSNRNNHLFWDLYHPTQEAHRMFANYIFDGPFTYPLNL 360

Query: 410 KQLIAL 412
            QLIAL
Sbjct: 361 NQLIAL 365

BLAST of MC10g1066 vs. ExPASy TrEMBL
Match: A0A6J1EWE7 (GDSL esterase/lipase At5g55050 OS=Cucurbita moschata OX=3662 GN=LOC111438588 PE=3 SV=1)

HSP 1 Score: 584 bits (1505), Expect = 2.20e-207
Identity = 289/364 (79.40%), Postives = 317/364 (87.09%), Query Frame = 0

Query: 50  MGSRAFFFFFIFLCVSFSFRVCECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNG 109
           MGS+  FFF   L   FS  + E  ALVPAIYVFGDSLVDVGNNNHLKLS+AKANFPHNG
Sbjct: 1   MGSQILFFFMFSLLSIFS-TISE-SALVPAIYVFGDSLVDVGNNNHLKLSIAKANFPHNG 60

Query: 110 IDFPTTKPTGRFSNGKNAADYLAEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGG 169
           IDFPT KPTGRFSNGKNAADY+AEKVGLPTSPPYLSL+SKFRK+ NT PFKTGVSFASGG
Sbjct: 61  IDFPTKKPTGRFSNGKNAADYIAEKVGLPTSPPYLSLISKFRKVINTKPFKTGVSFASGG 120

Query: 170 AGIFNETNNLFVSMLAMGQQIDFYSSVYQHLVEELGSSAAQVHLSKSLFTVVIGSNDIFG 229
           AGIFNETNNLF   +AM QQI++YS VYQ LV ELG SAAQ HLSKSLFTVVIGSNDIFG
Sbjct: 121 AGIFNETNNLFKQSVAMDQQIEYYSLVYQKLVVELGLSAAQAHLSKSLFTVVIGSNDIFG 180

Query: 230 YHESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQRKRSRTG 289
           YHESSDLRKKYSPQQYV+ MASTL+SQLKRLY YGARKYV+ G+GLVGCTPSQRKRS+TG
Sbjct: 181 YHESSDLRKKYSPQQYVESMASTLKSQLKRLYGYGARKYVVTGIGLVGCTPSQRKRSKTG 240

Query: 290 ECDAEVNSWVEHYNFGLKAMLGRLKMELNG-IAFSYFDAYAVMSNFIRTPSSYGFTEIRR 349
           ECD EVNSW   YN  LK+ML  LKMELN  I+F+YF+ YAVMS+FIR+PSS+GFTEI+ 
Sbjct: 241 ECDEEVNSWAAEYNVALKSMLQTLKMELNNNISFTYFEVYAVMSDFIRSPSSFGFTEIKS 300

Query: 350 ACCGLGNLNADVPCLPIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFDGPFTYALNVKQ 409
           ACCG G+LNAD+PCLPIAAFCSNRN HLFWDLYHPTQEA R+  +YIF+GPFTY +NV Q
Sbjct: 301 ACCGHGHLNADLPCLPIAAFCSNRNSHLFWDLYHPTQEASRVLADYIFNGPFTYPINVNQ 360

Query: 410 LIAL 412
           LIAL
Sbjct: 361 LIAL 362

BLAST of MC10g1066 vs. ExPASy TrEMBL
Match: A0A6J1HMQ2 (GDSL esterase/lipase At5g55050 OS=Cucurbita maxima OX=3661 GN=LOC111465054 PE=3 SV=1)

HSP 1 Score: 583 bits (1503), Expect = 4.44e-207
Identity = 287/364 (78.85%), Postives = 318/364 (87.36%), Query Frame = 0

Query: 50  MGSRAFFFFFIFLCVSFSFRVCECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNG 109
           MGS+  F F IF  +S    + E  ALVPAIYVFGDSLVDVGNNNHLKLS+AKANFPHNG
Sbjct: 1   MGSQLLFCF-IFSLLSIFSTISE-SALVPAIYVFGDSLVDVGNNNHLKLSIAKANFPHNG 60

Query: 110 IDFPTTKPTGRFSNGKNAADYLAEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGG 169
           IDFPT KPTGRFSNGKNAADY+AEKVGLPTSPPYLSL+SKFRK+ NT PFKTGVSFASGG
Sbjct: 61  IDFPTKKPTGRFSNGKNAADYIAEKVGLPTSPPYLSLISKFRKVINTKPFKTGVSFASGG 120

Query: 170 AGIFNETNNLFVSMLAMGQQIDFYSSVYQHLVEELGSSAAQVHLSKSLFTVVIGSNDIFG 229
           AGIFNETNNLF   +A+ QQI++YS VYQ+LV ELG SAA+ HLSKSLFTVVIGSNDIFG
Sbjct: 121 AGIFNETNNLFKQSVAIDQQIEYYSLVYQNLVVELGLSAAEAHLSKSLFTVVIGSNDIFG 180

Query: 230 YHESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQRKRSRTG 289
           YHESSDLRKKYSPQQYV+ MASTL+SQLKRLY YGARKYV+ G+GLVGCTPSQRKRS+TG
Sbjct: 181 YHESSDLRKKYSPQQYVESMASTLKSQLKRLYGYGARKYVVTGIGLVGCTPSQRKRSKTG 240

Query: 290 ECDAEVNSWVEHYNFGLKAMLGRLKMELNG-IAFSYFDAYAVMSNFIRTPSSYGFTEIRR 349
           ECD EVNSW   YN  LK+ML  LKMELN  I+FSYF+ YA+MS+F+R+PSSYGFTEIR 
Sbjct: 241 ECDVEVNSWAAQYNVALKSMLQTLKMELNNNISFSYFEVYAIMSDFVRSPSSYGFTEIRS 300

Query: 350 ACCGLGNLNADVPCLPIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFDGPFTYALNVKQ 409
           ACCG G+LNAD+PCLPIAAFCSNRN HLFWDLYHPTQEA R+  +YIF+GPFTY +NV Q
Sbjct: 301 ACCGHGHLNADLPCLPIAAFCSNRNSHLFWDLYHPTQEASRMLADYIFNGPFTYPINVNQ 360

Query: 410 LIAL 412
           LIAL
Sbjct: 361 LIAL 362

BLAST of MC10g1066 vs. ExPASy TrEMBL
Match: A0A0A0L695 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G075990 PE=3 SV=1)

HSP 1 Score: 578 bits (1491), Expect = 2.98e-205
Identity = 283/363 (77.96%), Postives = 308/363 (84.85%), Query Frame = 0

Query: 50  MGSRAFFFFFIFLCVSFSFRVCECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNG 109
           MGS+      + L  S   RV    +LVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNG
Sbjct: 1   MGSQLLLSLLLLLFFSSLIRVSN-SSLVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNG 60

Query: 110 IDFPTTKPTGRFSNGKNAADYLAEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGG 169
           +DFPT KPTGRFSNGKNAAD++AE+VGL TSPPYLSL+SKFRK  NT PFKTGVSFASGG
Sbjct: 61  LDFPTKKPTGRFSNGKNAADFVAERVGLATSPPYLSLISKFRKTVNTAPFKTGVSFASGG 120

Query: 170 AGIFNETNNLFVSMLAMGQQIDFYSSVYQHLVEELGSSAAQVHLSKSLFTVVIGSNDIFG 229
           AGIFNETNNLF   +AM QQI+ YS VY +LV ELGSS A  HLSKSLFT+VIGSNDIFG
Sbjct: 121 AGIFNETNNLFKQSVAMEQQIELYSRVYTNLVGELGSSGAAAHLSKSLFTIVIGSNDIFG 180

Query: 230 YHESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQRKRSRTG 289
           YHESSDLRKKYSPQQY+DLMASTL SQLKRL+ YGARKYV+GG+GLVGC PSQRKRS T 
Sbjct: 181 YHESSDLRKKYSPQQYLDLMASTLHSQLKRLHGYGARKYVVGGIGLVGCAPSQRKRSETE 240

Query: 290 ECDAEVNSWVEHYNFGLKAMLGRLKMELNGIAFSYFDAYAVMSNFIRTPSSYGFTEIRRA 349
           +CD EVN+W   YN  LK+ L  LKMELN I+FSYFD Y VMSNFI +PSSYGFTEI+ A
Sbjct: 241 DCDEEVNNWAAIYNTALKSKLETLKMELNDISFSYFDVYQVMSNFIHSPSSYGFTEIKSA 300

Query: 350 CCGLGNLNADVPCLPIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFDGPFTYALNVKQL 409
           CCGLG LNADVPCLPIA FCSNRN+HLFWDLYHPTQEAHR+F NYIFDGPFTY LN+KQL
Sbjct: 301 CCGLGKLNADVPCLPIAKFCSNRNNHLFWDLYHPTQEAHRMFANYIFDGPFTYPLNLKQL 360

Query: 410 IAL 412
           IAL
Sbjct: 361 IAL 362

BLAST of MC10g1066 vs. TAIR 10
Match: AT5G55050.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 347.8 bits (891), Expect = 1.2e-95
Identity = 174/342 (50.88%), Postives = 232/342 (67.84%), Query Frame = 0

Query: 75  ALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNGIDFPTTKPTGRFSNGKNAADYLAEK 134
           A +P +YVFGDSLVD GNNN+L +S++KAN+PHNG+DFP  KPTGRF NGKNAAD +AEK
Sbjct: 35  ASIPGLYVFGDSLVDAGNNNYLPISISKANYPHNGVDFPNKKPTGRFCNGKNAADAIAEK 94

Query: 135 VGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGGAGIFNETNNLFVSMLAMGQQIDFYS 194
            GLP  PPYLSL    ++    +   TGV+FASGGAGIFN ++      + + +Q++ + 
Sbjct: 95  FGLPLPPPYLSLRGLLKREKRKSAAVTGVNFASGGAGIFNSSDQKLGQAIPLSKQVNNWL 154

Query: 195 SVYQHLVEELGSSAAQVHLSKSLFTVVIGSNDIFGYHESSDLRKKYSPQQYVDLMASTLE 254
           S+++  V +L  SAAQ+HLSKSLFTVVIGSND+F Y  S  LR++ +PQQY  LMA  L+
Sbjct: 155 SIHEE-VMKLEPSAAQLHLSKSLFTVVIGSNDLFDYFGSFKLRRQSNPQQYTQLMADKLK 214

Query: 255 SQLKRLYSYGARKYVIGGVGLVGCTPSQR-KRSRTGECDAEVNSWVEHYNFGLKAMLGRL 314
            QLKR++  GAR+++I GV  +GCTP +R K S   ECD   N W   YN  L  ML +L
Sbjct: 215 EQLKRIHDSGARRFLIIGVAQIGCTPGKRAKNSTLHECDEGANMWCSLYNEALVKMLQQL 274

Query: 315 KMELNG-IAFSYFDAYAVMSNFIRTPSSYGFTEIRRACCGLGNLNADVPCLPIAAFCSNR 374
           K EL G I ++YFD Y  + + I  P+ YGF ++  ACCG G LNAD+PCLP+A  CS+R
Sbjct: 275 KQELQGSITYTYFDNYKSLHDIISNPARYGFADVTSACCGNGELNADLPCLPLAKLCSDR 334

Query: 375 NDHLFWDLY-HPTQEAHRLFGNYIF--DGPFTYALNVKQLIA 412
             HLFWD Y HPT+ A R   + +   D  ++  + + QL++
Sbjct: 335 TKHLFWDRYGHPTEAAARTIVDLMLTDDTHYSSPITLTQLVS 375

BLAST of MC10g1066 vs. TAIR 10
Match: AT5G37690.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 246.9 bits (629), Expect = 2.8e-65
Identity = 121/318 (38.05%), Postives = 188/318 (59.12%), Query Frame = 0

Query: 81  YVFGDSLVDVGNNNHLKLSLAKANFPHNGIDFPTTKPTGRFSNGKNAADYLAEKVGLPTS 140
           Y+FGDSL +VGNNN L+ SLA+A+FP+ G+DF   K TGRF+NG+   D ++ K+G+ + 
Sbjct: 29  YIFGDSLTEVGNNNFLQYSLARADFPYYGVDFSGGKATGRFTNGRTIGDIISTKLGILSP 88

Query: 141 PPYLSLVSKFRKITNTTPFKTGVSFASGGAGIFNETNNLFVSMLAMGQQIDFYSSVYQHL 200
           PPYLSL        N   F +G+++ASGGAGI NET   F+  L    QI+ +    + +
Sbjct: 89  PPYLSLSQ------NDDAFLSGINYASGGAGILNETGIYFIQRLTFNDQINCFKKTKEVI 148

Query: 201 VEELGSSAAQVHLSKSLFTVVIGSNDIFGYHESSDLR--KKYSPQQYVDLMASTLESQLK 260
             ++G  AA  H++ +++ + +GSND         +   ++Y+  ++V+L+ STL +QL 
Sbjct: 149 RAKIGDGAANKHVNDAMYFIGLGSNDYVNNFLQPFMADGQQYTHDEFVELLTSTLHNQLT 208

Query: 261 RLYSYGARKYVIGGVGLVGCTPSQRKRSRTGECDAEVNSWVEHYNFGLKAMLGRLKMELN 320
            +Y  GARK +  G+G +GC PSQR +S+T  C   VN WV  +N   K +L  L   L 
Sbjct: 209 TIYKLGARKVIFHGLGPLGCIPSQRVKSKTRMCLNRVNEWVLEFNSRTKKLLIDLNKRLP 268

Query: 321 GIAFSYFDAYAVMSNFIRTPSSYGFTEIRRACCGLGNLNADVPCLPIAAFCSNRNDHLFW 380
           G  FS+ D Y  + + I  P+ YGF     +CC + + +    CLP +  C NR D +FW
Sbjct: 269 GAKFSFADTYPAVLDLINNPTHYGFKIANTSCCNV-DTSVGGLCLPNSKMCKNRQDFVFW 328

Query: 381 DLYHPTQEAHRLFGNYIF 397
           D +HP+  A+++  +++F
Sbjct: 329 DAFHPSDSANQILADHLF 339

BLAST of MC10g1066 vs. TAIR 10
Match: AT5G08460.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 224.9 bits (572), Expect = 1.2e-58
Identity = 125/351 (35.61%), Postives = 186/351 (52.99%), Query Frame = 0

Query: 72  ECGALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNGIDFPTTKPTGRFSNGKNAADYL 131
           E  A+ PA++VFGDSLVD GNNNHL  SLA++N+   GIDF   +PTGRFSNGK   D++
Sbjct: 42  ETTAMFPAMFVFGDSLVDNGNNNHLN-SLARSNYLPYGIDFAGNQPTGRFSNGKTIVDFI 101

Query: 132 AEKVGLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGGAGIFNETNNLFVSMLAMGQQID 191
            E +GLP  P ++  V     I +      GV++AS   GI  ET        +MG+Q++
Sbjct: 102 GELLGLPEIPAFMDTVDGGVDILH------GVNYASAAGGILEETGRHLGERFSMGRQVE 161

Query: 192 FYSSVYQHLVEELGSSAAQVHLSKSLFTVVIGSNDIFGYHESSDL---RKKYSPQQYVDL 251
            +      +   +   + + +++KSL  V +G+ND    +    L      Y P  + DL
Sbjct: 162 NFEKTLMEISRSMRKESVKEYMAKSLVVVSLGNNDYINNYLKPRLFLSSSIYDPTSFADL 221

Query: 252 MASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQ--RKRSRTGECDAEVNSWVEHYNFGL 311
           + S   + L  LY  G RK+VI GVG +GC P Q   + +  GEC   VN   E +N  L
Sbjct: 222 LLSNFTTHLLELYGKGFRKFVIAGVGPLGCIPDQLAAQAALPGECVEAVNEMAELFNNRL 281

Query: 312 KAMLGRLKME---LNGIAFSYFDAYAVMSNFIRTPSSYGFTEIRRACCGLGNLNADVPCL 371
            +++ RL  +    +   F Y + Y    + +  P +YGF    R CCG+G    ++ CL
Sbjct: 282 VSLVDRLNSDNKTASEAIFVYGNTYGAAVDILTNPFNYGFEVTDRGCCGVGRNRGEITCL 341

Query: 372 PIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIFDGPFT--YALNVKQLIAL 413
           P+A  C+ R+ H+FWD +HPTQ  + +     F+G  +  Y +N+ QL  L
Sbjct: 342 PLAVPCAFRDRHVFWDAFHPTQAFNLIIALRAFNGSKSDCYPINLSQLSRL 385

BLAST of MC10g1066 vs. TAIR 10
Match: AT1G71691.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 223.0 bits (567), Expect = 4.4e-58
Identity = 121/342 (35.38%), Postives = 182/342 (53.22%), Query Frame = 0

Query: 76  LVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNGIDFPTTKPTGRFSNGKNAADYLAEKV 135
           +VPA++VFGDSL+D GNNN++  S AKAN+   GIDF    PTGRF NG    D +A+ +
Sbjct: 52  IVPALFVFGDSLIDNGNNNNIP-SFAKANYFPYGIDF-NGGPTGRFCNGLTMVDGIAQLL 111

Query: 136 GLPTSPPYLSLVSKFRKITNTTPFKTGVSFASGGAGIFNETNNLFVSMLAMGQQIDFYSS 195
           GLP  P Y                  GV++AS  AGI  +T   FV  +   QQI  + +
Sbjct: 112 GLPLIPAYSEATG--------DQVLRGVNYASAAAGILPDTGGNFVGRIPFDQQIHNFET 171

Query: 196 VYQHLVEELGSSAAQV-HLSKSLFTVVIGSNDIFGYH--ESSDLRKKYSPQQYVDLMAST 255
               +  + G + A    +++SLF + +GSND    +   +   R +Y+ QQ+ DL+   
Sbjct: 172 TLDQVASKSGGAVAIADSVTRSLFFIGMGSNDYLNNYLMPNFPTRNQYNSQQFGDLLVQH 231

Query: 256 LESQLKRLYSYGARKYVIGGVGLVGCTPSQRKRSRTGECDAEVNSWVEHYNFGLKAMLGR 315
              QL RLY+ G RK+V+ G+G +GC PS   +   G+C  EVN  V  +N  +K M+  
Sbjct: 232 YTDQLTRLYNLGGRKFVVAGLGRMGCIPSILAQGNDGKCSEEVNQLVLPFNTNVKTMISN 291

Query: 316 LKMELNGIAFSYFDAYAVMSNFIRTPSSYGFTEIRRACCGLGNLNADVPCLPIAAFCSNR 375
           L   L    F Y D   +  + +   ++YG T + + CCG+G     + CLP    C NR
Sbjct: 292 LNQNLPDAKFIYLDIAHMFEDIVANQAAYGLTTMDKGCCGIGKNRGQITCLPFETPCPNR 351

Query: 376 NDHLFWDLYHPTQEAHRLFGNYIFDGPFT--YALNVKQLIAL 413
           + ++FWD +HPT++ + +     F G  T  Y +N++QL +L
Sbjct: 352 DQYVFWDAFHPTEKVNLIMAKKAFAGDRTVAYPINIQQLASL 383

BLAST of MC10g1066 vs. TAIR 10
Match: AT3G50400.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 221.5 bits (563), Expect = 1.3e-57
Identity = 141/373 (37.80%), Postives = 207/373 (55.50%), Query Frame = 0

Query: 58  FFIFLCVSFSF-----RVCECG--ALVPAIYVFGDSLVDVGNNNHLKLSLAKANFPHNGI 117
           FF+ + V F F     RV   G    + A +VFGDSLVD GNNN+L+ +L++AN P NGI
Sbjct: 6   FFVPVLVLFFFGSRFSRVASAGDQRALAASFVFGDSLVDAGNNNYLQ-TLSRANSPPNGI 65

Query: 118 DFPTTK--PTGRFSNGKNAADYLAEKVGLPT-SPPYLSLVSKFRKITNTTPFKTGVSFAS 177
           DF  ++  PTGRF+NG+  AD + EK+G  + + PYL+  +    + N      GV++AS
Sbjct: 66  DFKPSRGNPTGRFTNGRTIADIVGEKLGQQSYAVPYLAPNASGEALLN------GVNYAS 125

Query: 178 GGAGIFNETNNLFVSMLAMGQQIDFYSSVYQHLVEELGSSAAQVHLSK-SLFTVVIGSND 237
           GG GI N T ++FV+ L M  Q+D++++  +   + LG   A+ ++ K SLF+VVIGSND
Sbjct: 126 GGGGILNATGSVFVNRLGMDIQVDYFTNTRKQFDKLLGQDKARDYIRKRSLFSVVIGSND 185

Query: 238 IFGYH----ESSDLRKKYSPQQYVDLMASTLESQLKRLYSYGARKYVIGGVGLVGCTPSQ 297
               +     ++  R   +P+ +VD M S L +QLKRLY   ARK+V+G V  +GC P Q
Sbjct: 186 FLNNYLVPFVAAQARLTQTPETFVDDMISHLRNQLKRLYDMDARKFVVGNVAPIGCIPYQ 245

Query: 298 RKRSRTG--ECDAEVNSWVEHYNFGLKAMLG-RLKMELNGIAFSYFDAYAVMSNFIRTPS 357
           +  ++    +C    N     YN  LK +L   LK  L    F Y + Y +  + I    
Sbjct: 246 KSINQLNDKQCVDLANKLAIQYNARLKDLLTVELKDSLKDAHFVYANVYDLFMDLIVNFK 305

Query: 358 SYGFTEIRRACCGL-GNLNADVPCLPIAAFCSNRNDHLFWDLYHPTQEAHRLFGNYIF-- 410
            YGF     ACC   G L   +PC P ++ C++R+ H+FWD YHPT+ A+ L  + +   
Sbjct: 306 DYGFRTASEACCETRGRLAGILPCGPTSSLCTDRSKHVFWDAYHPTEAANLLIADKLLYG 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FIA11.7e-9450.88GDSL esterase/lipase At5g55050 OS=Arabidopsis thaliana OX=3702 GN=At5g55050 PE=2... [more]
Q9FHQ14.0e-6438.05GDSL esterase/lipase At5g37690 OS=Arabidopsis thaliana OX=3702 GN=At5g37690 PE=2... [more]
O234703.5e-6037.00GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3... [more]
Q9FNP21.6e-5735.61GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana OX=3702 GN=At5g08460 PE=2... [more]
Q9SF786.2e-5735.38GDSL esterase/lipase At1g71691 OS=Arabidopsis thaliana OX=3702 GN=At1g71691 PE=2... [more]
Match NameE-valueIdentityDescription
XP_022151286.11.88e-26698.90GDSL esterase/lipase At5g55050-like [Momordica charantia][more]
XP_008449153.11.53e-20878.42PREDICTED: GDSL esterase/lipase At5g55050 [Cucumis melo][more]
XP_022932219.14.55e-20779.40GDSL esterase/lipase At5g55050 [Cucurbita moschata][more]
XP_022965085.19.18e-20778.85GDSL esterase/lipase At5g55050 [Cucurbita maxima][more]
XP_023552078.11.78e-20679.40GDSL esterase/lipase At5g55050 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1DE659.09e-26798.90GDSL esterase/lipase At5g55050-like OS=Momordica charantia OX=3673 GN=LOC1110192... [more]
A0A1S3BM177.40e-20978.42GDSL esterase/lipase At5g55050 OS=Cucumis melo OX=3656 GN=LOC103491100 PE=3 SV=1[more]
A0A6J1EWE72.20e-20779.40GDSL esterase/lipase At5g55050 OS=Cucurbita moschata OX=3662 GN=LOC111438588 PE=... [more]
A0A6J1HMQ24.44e-20778.85GDSL esterase/lipase At5g55050 OS=Cucurbita maxima OX=3661 GN=LOC111465054 PE=3 ... [more]
A0A0A0L6952.98e-20577.96Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G075990 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G55050.11.2e-9550.88GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G37690.12.8e-6538.05SGNH hydrolase-type esterase superfamily protein [more]
AT5G08460.11.2e-5835.61GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G71691.24.4e-5835.38GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT3G50400.11.3e-5737.80GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 72..398
e-value: 6.0E-83
score: 280.9
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 80..395
e-value: 1.6E-26
score: 94.1
NoneNo IPR availablePANTHERPTHR45648GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)coord: 63..410
NoneNo IPR availablePANTHERPTHR45648:SF106ANTHER-SPECIFIC PROLINE-RICH PROTEIN APGcoord: 63..410
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 79..397
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 78..399
e-value: 1.86032E-124
score: 360.777

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g1066.1MC10g1066.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0016788 hydrolase activity, acting on ester bonds