MC10g0817 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g0817
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Serine hydroxymethyltransferase
Location	MC10: 6907962 .. 6912050 (+)
RNA-Seq Expression	MC10g0817
Synteny	MC10g0817

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

ATACATGTCAATTACCTTTTAACTATGCTCGTGTTGACAACTAATCTACTGTGAAATCGATTATTGTGATTCTAACGACCATGTACAACTTTAGAGTTTGATTTTAATGAACGAAATGGATAACTCTTGTCACGAATGTAAAATTATGGAATATACTTTGAGGGCCAAATTTGTAAATGGACTTTTTATTAAATTATAGATTTGAAAGGATATTGACAACTGCTGACATGGAAAATACATATCCGATAAATTCTTATTAAAGAATTACTAATTAGTCCTTGAGCCAAACGAATCAGAAAATTTTCGATTATTTAAAATTGGTCAGAACCCTACGCTTTCGGCCACTCTATATTCGAAGCCAAAAGCACGAAGAACGCACAAAAAACACAACGGGGGAAAAAAAAGTCTCAGCCGCTACGCTTCCGACCCTCCGCCGCCTCCTCCGCCTCCTCCGCCGTCATCTCGACCCCTCCACTTCACCGGTTCAAGGTTCCGTTCTGCCATCACTGAATCGTTATCTCGCTTTCTTTTAGCTTTTTTTTCCCACTATTTCTGTGTTGAAGTATTGGAATGTTGGCGATGAACGATTAATATTGTTTGTTTTCACCTAAAAAGAATTAAGCAGCGCGCTTCGAAGTGTGTGTGGTTAATATGACTATGTTGCTTGCTGTTTTTGGACTTTTCATGTTTATTGAAGTTATTGTTGTCCTGTAATTTGTAATCGAGGCTCTGTTATGGCGTTTAATGGTTACTGAAGTTCGTTTTCCTTTATGAAATGGGTAGGAGTTTTGTGAAGGTTTAAGTGGACAGAATGCAGGCTGCGAGTGGTGCTTCTATGATGGGTTCTCTGCAAGTTGTTGTTTGTGCGAAGGGATCGGCTTTTCCTTCGAAAGGGCCTAGCATCTGTGTGTTTCCACAACCGAAGAAACTGAATATCTTGAAACCTTGTAAATCCTCGAATGTTGAAGCAAGCGCAGTTGCAGGAAGGTCATCTGTCTCTGTCACTGTTCCTGAAATTGGAGGTGAAAACTATTATCTAATTGTCATCAAAGTTATCATTTGTTTGAAAGAAGTCTAGATTTCGCCCCTCTACTAGGAGGAAGAAATTGTGCAAGATTTCTTTTGTTTTTTTAATACCATACAAGCACAATTGATCACTGGGAATGAAAAGAATTTGATTTTGATTGAGTGGTAGAACTTAGCTTAGGTTAACTTCCTCGCCGTTATGCAGGTGGAAGCCACTTGGTAGACCATGAATTGAGTGAAACTGATCCCGAAGTTCGATCTATTATTAACAAAGAAAAGCAGCGACAATTTAAAAGCCTTGAACTTATTGCCTCAGAAAACTTTACATCTCGGGCAGTTATGGAGGCAGTAGGGTCATGTCTCACGAACAAGTATTCAGAAGGATTACCTGGCAAAAGGTGAACTTTCTTCATTTGATGATACTTGGATGTGAATTCAGGTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGTTTTNGGGGTTATTGCAAATTAAGATTTTTATTATGCATTTCAGGTATTATGGTGGCAATGAGCATATTGATGAGCTTGAAACACTCTGCCAACAAAGGGCTCTAGCAGCCTTTCATTTGGATGAGAACAAGTGGGGTGTAAATGTTCAACCATTATCTGGATCACCTGCTAACTTTGAGGTTTATACTGCAGTTCTTAGTCCACACGATCGGATCATGGTGAGATGATTGTTTTTTGGTCCCCATATTAAATCAGACAACCATCAACTTTACTAATTTGTGTTAAGTGACTTTAGGGTTTAGACTTACCTCATGGAGGACATTTGTCTCACGGGTTTATGACACCAAAAAGGCGGGTATCAGGCACATCAATTTATTTCGAATCTATGCCTTATCGACTTGATGAGACTACAGGTACTTCCCATGTGGGATAAGGCTTTTGTTATGGTCTCTTTCTCGGTTTATGTATTATAATTAATTAGCAAACTCTTACAGGTATCGTAGATTATGACATGCTTGAAAAAACTGCTAACTTGTTTCGACCAAAGTTAATTATTGCTGGTGCAAGTGCTTATCCACGAGATTTTGACTATCCTCGCATGAGAAAAGTAAGGATTTTAAATCCTGTGTTAAGCATTTTACATTAAGAAGCATTGTTTAGACAATAGGTTCCATTAGTGAATAACTTATTTGATTCGTTCCGGTAGATTGCGGATGCTGTTGGTGCTTTTCTCATGATGGATATGGCTCACATAAGTGGGCTTGTTGCTGCTTCGGTAGTTGCTGATCCTTTTGAATACTGTGATATTGTGACAACGACAACACATAAGGTAAAGGATAATTTTTGTTTTTGTCTATGGGTGCATCATTTTAAGTACCACGTTTTCCTCTAAATTCATTTAATTTTTTAAAAACATAAGAAAGGGGGTATATTCAAGAACCAAACTTATTTACCAGAGTAGATAAAGCCCCCACCGGGATAAAACTACTATAAAAGAAATCTTGAGTCCAAGTTTATGTAATGATTTCTTTCTATCTTGGAAGATACTTCGGTTTATTTTTAGCCATAGAATCCTCAAAAGAAGCTCTAACTTCACAATTCACCAACCACTTTTGTCTTGTCTTTTAACTACCAGCCCGTGGGGTCTTCAAGCAGCCAACCCGAAAGAGAGCAGAGGAATACTAAATTCGTTTCATAGTCATTTACCTATTTTGGAAGCGTTTTTCAGTCTTATCTTGAAACAAGCGTAACTTTATATATCATTTAGGACACGTTTAGATCATCACTCTCAGAAATGCCCTTGTTCTTCGCATTAAAGAAACTGCTTTCTCAACCTGAAATAATTGTCAAAAAATTGTTTGCAGTCTCTGAGAGGTCCAAGAGGTGGCATGATATTTTTCAGGAAGGATCCAATTCTCGGAGTTGACTTAGAATCTGCAATTAATAATGCCGTGTTCCCAGGATTACAGGTTCGTTTGACTGGCCTTGTGTTGTGGTAGCACGTAAAGAAGTGTTCATTCGTTTTGCTAGATACTTCGTCTTCGATTTCTAAACATTGTCAATTTTATGGCTGTTGGGCAGGGTGGTCCTCATAATCATACAATTGGGGGACTTGCTGTCTGCTTGAAGCATGCACAATCCCCAGAATTTAGGGTCTATCAGAATAAGGTACTGCACTTCCTCAATGAGAACATGTGGTGTAAGACACTGTTTTCTTATTTTCTAACAGAGCATTTTCATACTCTTAGGTCGTCTCCAACTGTAGAGCTCTTGCGAGTCGACTTGTCGAGTTAGGTTATAAGCTAGTTTCTGGTGGAAGTGACAATCACCTGGTTCTTGTGGATCTCAGGCCATTGGTAAATTTTTGCGTCTGGATAACAATTTTACGTTTTCTCTCTCCAATAAATTGATGACACCCGGGGATATACTCTAACAACAGGGTATCGATGGGGCGCGGGTGGAGAAAATTCTCGACATGGCCTCGATCACTCTCAACAAGAACTCTGTACCCGGTAATGAAGTGTTCCAATTCCAACAGGCCAATAGTCATATCGAATTTTCTTGAACTAACTCTTAGTTGTTCTATACATAGGTGACAAGAGTGCTCTAGTTCCTGGAGGCATTCGTATCGGATCGCCTGCCATGACCACCCGAGGATTCACCGAGAAAGAATTCATAGCAGTTGCAGACTTCATCCATGAGGGTGTTCAAATAACTCTTAATGCCAAACAACTTGCCCCAGGATCAAAGCTCCAAGAGTTCCTCAAGTTTGTGAAAACCACTGATTTCCCTCTCGTGGATCGAGTTTCAGAGCTTCGCTCAAGAGTTGAAGCACTCACAACTCAATTTCCAATACCAGGTCTGTAAGTCTGAGTTTCACTCCATATAGGGAAATGGGTATAAGCTTACAATAAAAATTCTGACAAAAAAAGGGGACCTTTTGTTGATTGTAGTAATAAAAACCACATAGTTTGTAACTTTTTATTTATAAGCTGTTGCTGCAGCAATGAGGCTTTTGCTTAGATTCCTTTTTCTAGATTTTATTTTTATATTTTCTTCCTGTCTGTTACACTTACTATTTGGATGTGTT

mRNA sequence

ATACATGTCAATTACCTTTTAACTATGCTCGTGTTGACAACTAATCTACTGTGAAATCGATTATTGTGATTCTAACGACCATGTACAACTTTAGAGTTTGATTTTAATGAACGAAATGGATAACTCTTGTCACGAATGTAAAATTATGGAATATACTTTGAGGGCCAAATTTGTAAATGGACTTTTTATTAAATTATAGATTTGAAAGGATATTGACAACTGCTGACATGGAAAATACATATCCGATAAATTCTTATTAAAGAATTACTAATTAGTCCTTGAGCCAAACGAATCAGAAAATTTTCGATTATTTAAAATTGGTCAGAACCCTACGCTTTCGGCCACTCTATATTCGAAGCCAAAAGCACGAAGAACGCACAAAAAACACAACGGGGGAAAAAAAAGTCTCAGCCGCTACGCTTCCGACCCTCCGCCGCCTCCTCCGCCTCCTCCGCCGTCATCTCGACCCCTCCACTTCACCGGTTCAAGGAGTTTTGTGAAGGTTTAAGTGGACAGAATGCAGGCTGCGAGTGGTGCTTCTATGATGGGTTCTCTGCAAGTTGTTGTTTGTGCGAAGGGATCGGCTTTTCCTTCGAAAGGGCCTAGCATCTGTGTGTTTCCACAACCGAAGAAACTGAATATCTTGAAACCTTGTAAATCCTCGAATGTTGAAGCAAGCGCAGTTGCAGGAAGGTCATCTGTCTCTGTCACTGTTCCTGAAATTGGAGGTGGAAGCCACTTGGTAGACCATGAATTGAGTGAAACTGATCCCGAAGTTCGATCTATTATTAACAAAGAAAAGCAGCGACAATTTAAAAGCCTTGAACTTATTGCCTCAGAAAACTTTACATCTCGGGCAGTTATGGAGGCAGTAGGGTCATGTCTCACGAACAAGTATTCAGAAGGATTACCTGGCAAAAGGTATTATGGTGGCAATGAGCATATTGATGAGCTTGAAACACTCTGCCAACAAAGGGCTCTAGCAGCCTTTCATTTGGATGAGAACAAGTGGGGTGTAAATGTTCAACCATTATCTGGATCACCTGCTAACTTTGAGGTTTATACTGCAGTTCTTAGTCCACACGATCGGATCATGGGTTTAGACTTACCTCATGGAGGACATTTGTCTCACGGGTTTATGACACCAAAAAGGCGGGTATCAGGCACATCAATTTATTTCGAATCTATGCCTTATCGACTTGATGAGACTACAGGTATCGTAGATTATGACATGCTTGAAAAAACTGCTAACTTGTTTCGACCAAAGTTAATTATTGCTGGTGCAAGTGCTTATCCACGAGATTTTGACTATCCTCGCATGAGAAAAATTGCGGATGCTGTTGGTGCTTTTCTCATGATGGATATGGCTCACATAAGTGGGCTTGTTGCTGCTTCGGTAGTTGCTGATCCTTTTGAATACTGTGATATTGTGACAACGACAACACATAAGTCTCTGAGAGGTCCAAGAGGTGGCATGATATTTTTCAGGAAGGATCCAATTCTCGGAGTTGACTTAGAATCTGCAATTAATAATGCCGTGTTCCCAGGATTACAGGGTGGTCCTCATAATCATACAATTGGGGGACTTGCTGTCTGCTTGAAGCATGCACAATCCCCAGAATTTAGGGTCTATCAGAATAAGGTCGTCTCCAACTGTAGAGCTCTTGCGAGTCGACTTGTCGAGTTAGGTTATAAGCTAGTTTCTGGTGGAAGTGACAATCACCTGGTTCTTGTGGATCTCAGGCCATTGGGTATCGATGGGGCGCGGGTGGAGAAAATTCTCGACATGGCCTCGATCACTCTCAACAAGAACTCTGTACCCGGTGACAAGAGTGCTCTAGTTCCTGGAGGCATTCGTATCGGATCGCCTGCCATGACCACCCGAGGATTCACCGAGAAAGAATTCATAGCAGTTGCAGACTTCATCCATGAGGGTGTTCAAATAACTCTTAATGCCAAACAACTTGCCCCAGGATCAAAGCTCCAAGAGTTCCTCAAGTTTGTGAAAACCACTGATTTCCCTCTCGTGGATCGAGTTTCAGAGCTTCGCTCAAGAGTTGAAGCACTCACAACTCAATTTCCAATACCAGGTCTGTAAGTCTGAGTTTCACTCCATATAGGGAAATGGGTATAAGCTTACAATAAAAATTCTGACAAAAAAAGGGGACCTTTTGTTGATTGTAGTAATAAAAACCACATAGTTTGTAACTTTTTATTTATAAGCTGTTGCTGCAGCAATGAGGCTTTTGCTTAGATTCCTTTTTCTAGATTTTATTTTTATATTTTCTTCCTGTCTGTTACACTTACTATTTGGATGTGTT

Coding sequence (CDS)

ATGCAGGCTGCGAGTGGTGCTTCTATGATGGGTTCTCTGCAAGTTGTTGTTTGTGCGAAGGGATCGGCTTTTCCTTCGAAAGGGCCTAGCATCTGTGTGTTTCCACAACCGAAGAAACTGAATATCTTGAAACCTTGTAAATCCTCGAATGTTGAAGCAAGCGCAGTTGCAGGAAGGTCATCTGTCTCTGTCACTGTTCCTGAAATTGGAGGTGGAAGCCACTTGGTAGACCATGAATTGAGTGAAACTGATCCCGAAGTTCGATCTATTATTAACAAAGAAAAGCAGCGACAATTTAAAAGCCTTGAACTTATTGCCTCAGAAAACTTTACATCTCGGGCAGTTATGGAGGCAGTAGGGTCATGTCTCACGAACAAGTATTCAGAAGGATTACCTGGCAAAAGGTATTATGGTGGCAATGAGCATATTGATGAGCTTGAAACACTCTGCCAACAAAGGGCTCTAGCAGCCTTTCATTTGGATGAGAACAAGTGGGGTGTAAATGTTCAACCATTATCTGGATCACCTGCTAACTTTGAGGTTTATACTGCAGTTCTTAGTCCACACGATCGGATCATGGGTTTAGACTTACCTCATGGAGGACATTTGTCTCACGGGTTTATGACACCAAAAAGGCGGGTATCAGGCACATCAATTTATTTCGAATCTATGCCTTATCGACTTGATGAGACTACAGGTATCGTAGATTATGACATGCTTGAAAAAACTGCTAACTTGTTTCGACCAAAGTTAATTATTGCTGGTGCAAGTGCTTATCCACGAGATTTTGACTATCCTCGCATGAGAAAAATTGCGGATGCTGTTGGTGCTTTTCTCATGATGGATATGGCTCACATAAGTGGGCTTGTTGCTGCTTCGGTAGTTGCTGATCCTTTTGAATACTGTGATATTGTGACAACGACAACACATAAGTCTCTGAGAGGTCCAAGAGGTGGCATGATATTTTTCAGGAAGGATCCAATTCTCGGAGTTGACTTAGAATCTGCAATTAATAATGCCGTGTTCCCAGGATTACAGGGTGGTCCTCATAATCATACAATTGGGGGACTTGCTGTCTGCTTGAAGCATGCACAATCCCCAGAATTTAGGGTCTATCAGAATAAGGTCGTCTCCAACTGTAGAGCTCTTGCGAGTCGACTTGTCGAGTTAGGTTATAAGCTAGTTTCTGGTGGAAGTGACAATCACCTGGTTCTTGTGGATCTCAGGCCATTGGGTATCGATGGGGCGCGGGTGGAGAAAATTCTCGACATGGCCTCGATCACTCTCAACAAGAACTCTGTACCCGGTGACAAGAGTGCTCTAGTTCCTGGAGGCATTCGTATCGGATCGCCTGCCATGACCACCCGAGGATTCACCGAGAAAGAATTCATAGCAGTTGCAGACTTCATCCATGAGGGTGTTCAAATAACTCTTAATGCCAAACAACTTGCCCCAGGATCAAAGCTCCAAGAGTTCCTCAAGTTTGTGAAAACCACTGATTTCCCTCTCGTGGATCGAGTTTCAGAGCTTCGCTCAAGAGTTGAAGCACTCACAACTCAATTTCCAATACCAGGTCTGTAA

Protein sequence

MQAASGASMMGSLQVVVCAKGSAFPSKGPSICVFPQPKKLNILKPCKSSNVEASAVAGRSSVSVTVPEIGGGSHLVDHELSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPANFEVYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYDMLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADPFEYCDIVTTTTHKSLRGPRGGMIFFRKDPILGVDLESAINNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQITLNAKQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPGL

Homology

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BLAST of MC10g0817 vs. ExPASy Swiss-Prot
Match: Q94JQ3 (Serine hydroxymethyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SHM3 PE=1 SV=2)

HSP 1 Score: 839.0 bits (2166), Expect = 3.0e-242
Identity = 418/529 (79.02%), Postives = 460/529 (86.96%), Query Frame = 0


Query: 1   MQAASGASMMGSLQVVVCAKGSAFPSKGPSICVFPQPKKLNILKPCKSSNVEASAVAGRS 60
           MQA  G + M SLQ     +GS FP   P +  F Q  K NI KP +SS ++ + V+   
Sbjct: 1   MQACCGGNSMASLQQPGRVQGSVFPPIMPPVTKFSQQLKFNISKPFRSSFLKRNLVSEMR 60

Query: 61  SVSVTVPEIGGGSHLV---DHELSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVME 120
           + SV++P +   S  +   D+ L E DPEVR+II KEK RQF+SLELIASENFTSRAVME
Sbjct: 61  ASSVSLPNVEISSKEIPFEDYGLGEVDPEVRTIITKEKDRQFRSLELIASENFTSRAVME 120

Query: 121 AVGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPA 180
           AVGSCLTNKYSEGLPGKRYYGGNE+ID+LETLCQ RALAAF LD  KWGVNVQPLSGSPA
Sbjct: 121 AVGSCLTNKYSEGLPGKRYYGGNEYIDQLETLCQNRALAAFRLDSTKWGVNVQPLSGSPA 180

Query: 181 NFEVYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDY 240
           NF VYTA+LSPHDRIMGLDLPHGGHLSHGFMT KRRVSGTSIYFESMPYRLDE+TGIVDY
Sbjct: 181 NFAVYTAILSPHDRIMGLDLPHGGHLSHGFMTAKRRVSGTSIYFESMPYRLDESTGIVDY 240

Query: 241 DMLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVAD 300
           DMLEKTA LFRPKLIIAGASAY RDFDYPRMRKIAD+VGAFLMMDMAHISGLVAASVVAD
Sbjct: 241 DMLEKTATLFRPKLIIAGASAYSRDFDYPRMRKIADSVGAFLMMDMAHISGLVAASVVAD 300

Query: 301 PFEYCDIVTTTTHKSLRGPRGGMIFFRKDPILGVDLESAINNAVFPGLQGGPHNHTIGGL 360
           PFEYCDIVTTTTHKSLRGPRGGMIFFRKDPI GVDLESA+NNAVFPGLQGGPHNHTIGGL
Sbjct: 301 PFEYCDIVTTTTHKSLRGPRGGMIFFRKDPINGVDLESAVNNAVFPGLQGGPHNHTIGGL 360

Query: 361 AVCLKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGAR 420
           AVCLKHAQSPEF+ YQ +VVSNCRALA+RLVELG+KLVSGGSDNHLVLVDLRP+G+DGAR
Sbjct: 361 AVCLKHAQSPEFKAYQKRVVSNCRALANRLVELGFKLVSGGSDNHLVLVDLRPMGMDGAR 420

Query: 421 VEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQIT 480
           VEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRG +EK+F+ VADFI EGV+IT
Sbjct: 421 VEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGLSEKDFVVVADFIKEGVEIT 480

Query: 481 LNAKQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPGL 527
           + AK+ APGSKLQ+F KFV + +FPL +RV  L+ RVE  T++FPIPG+
Sbjct: 481 MEAKKAAPGSKLQDFNKFVTSPEFPLKERVKSLKERVETFTSRFPIPGV 529

[Close]

BLAST of MC10g0817 vs. ExPASy Swiss-Prot
Match: Q9SZJ5 (Serine hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SHM1 PE=1 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 3.7e-163
Identity = 284/488 (58.20%), Postives = 358/488 (73.36%), Query Frame = 0


Query: 46  CKSSNVEASAVAGRSSVSVTVPEIGGGSHLVDHELSETDPEVRSIINKEKQRQFKSLELI 105
           C  S++ + AV  +    VT P+       ++  L E DPE+  II  EK RQ+K LELI
Sbjct: 27  CYMSSLPSEAVDEKERSRVTWPK------QLNAPLEEVDPEIADIIEHEKARQWKGLELI 86

Query: 106 ASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKW 165
            SENFTS +VM+AVGS +TNKYSEG PG RYYGGNE+ID  ETLCQ+RAL AF LD  KW
Sbjct: 87  PSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAETLCQKRALEAFRLDPEKW 146

Query: 166 GVNVQPLSGSPANFEVYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMP 225
           GVNVQPLSGSPANF VYTA+L PH+RIM LDLPHGGHLSHG+ T  +++S  SI+FE+MP
Sbjct: 147 GVNVQPLSGSPANFHVYTALLKPHERIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMP 206

Query: 226 YRLDETTGIVDYDMLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAH 285
           YRLDE+TG +DYD +EK+A LFRPKLI+AGASAY R +DY R+RK+ +   A ++ DMAH
Sbjct: 207 YRLDESTGYIDYDQMEKSATLFRPKLIVAGASAYARLYDYARIRKVCNKQKAVMLADMAH 266

Query: 286 ISGLVAASVVADPFEYCDIVTTTTHKSLRGPRGGMIFFRK--------DPILGVDLESAI 345
           ISGLVAA+V+  PF+Y D+VTTTTHKSLRGPRG MIFFRK           +  D E  I
Sbjct: 267 ISGLVAANVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKGVKEINKQGKEVLYDFEDKI 326

Query: 346 NNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSG 405
           N AVFPGLQGGPHNHTI GLAV LK A + E++ YQ +V+SN    A  L+E GY+LVSG
Sbjct: 327 NQAVFPGLQGGPHNHTITGLAVALKQATTSEYKAYQEQVLSNSAKFAQTLMERGYELVSG 386

Query: 406 GSDNHLVLVDLRPLGIDGARVEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRG 465
           G+DNHLVLV+L+P GIDG+RVEK+L+   I  NKN+VPGD SA+VPGGIR+G+PA+T+RG
Sbjct: 387 GTDNHLVLVNLKPKGIDGSRVEKVLEAVHIASNKNTVPGDVSAMVPGGIRMGTPALTSRG 446

Query: 466 FTEKEFIAVADFIHEGVQITLNAKQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEAL 525
           F E++F  VA++  + V I L  K  A G+KL++F+  ++++   +   +++LR  VE  
Sbjct: 447 FVEEDFAKVAEYFDKAVTIALKVKSEAQGTKLKDFVSAMESSS-TIQSEIAKLRHEVEEF 506

[Close]

BLAST of MC10g0817 vs. ExPASy Swiss-Prot
Match: P34899 (Serine hydroxymethyltransferase, mitochondrial OS=Pisum sativum OX=3888 PE=1 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 8.2e-163
Identity = 284/485 (58.56%), Postives = 357/485 (73.61%), Query Frame = 0


Query: 49  SNVEASAVAGRSSVSVTVPEIGGGSHLVDHELSETDPEVRSIINKEKQRQFKSLELIASE 108
           S++   AV  + +  VT P+       ++  L   DPE+  II  EK RQ+K LELI SE
Sbjct: 31  SSLPDEAVYDKENPRVTWPK------QLNSPLEVIDPEIADIIELEKARQWKGLELIPSE 90

Query: 109 NFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKWGVN 168
           NFTS +VM+AVGS +TNKYSEG PG RYYGGNE+ID  ETLCQ+RAL AF LD  KWGVN
Sbjct: 91  NFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAETLCQKRALEAFRLDPAKWGVN 150

Query: 169 VQPLSGSPANFEVYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRL 228
           VQPLSGSP+NF+VYTA+L PHDRIM LDLPHGGHLSHG+ T  +++S  SI+FE+MPYRL
Sbjct: 151 VQPLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRL 210

Query: 229 DETTGIVDYDMLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISG 288
           DE+TG +DYD LEK+A LFRPKLI+AGASAY R +DY R+RK+ D   A L+ DMAHISG
Sbjct: 211 DESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDYARIRKVCDKQKAVLLADMAHISG 270

Query: 289 LVAASVVADPFEYCDIVTTTTHKSLRGPRGGMIFFRK--------DPILGVDLESAINNA 348
           LVAA V+  PF+Y D+VTTTTHKSLRGPRG MIFFRK           +  D E  IN A
Sbjct: 271 LVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKGLKEVNKQGKEVFYDYEDKINQA 330

Query: 349 VFPGLQGGPHNHTIGGLAVCLKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSD 408
           VFPGLQGGPHNHTI GLAV LK A +PE+R YQ +V+SN    A  L E GY LVSGG++
Sbjct: 331 VFPGLQGGPHNHTITGLAVALKQATTPEYRAYQEQVLSNSSKFAKALSEKGYDLVSGGTE 390

Query: 409 NHLVLVDLRPLGIDGARVEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTE 468
           NHLVLV+L+  GIDG+RVEK+L++  I  NKN+VPGD SA+VPGGIR+G+PA+T+RGF E
Sbjct: 391 NHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVE 450

Query: 469 KEFIAVADFIHEGVQITLNAKQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQ 526
           ++F+ VA++    V + L  K  + G+KL++F++ ++T+ + +   +S+L+  VE    Q
Sbjct: 451 EDFVKVAEYFDAAVSLALKVKAESKGTKLKDFVEALQTSSY-VQSEISKLKHDVEEFAKQ 508

[Close]

BLAST of MC10g0817 vs. ExPASy Swiss-Prot
Match: P34898 (Serine hydroxymethyltransferase, cytosolic OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=for PE=3 SV=2)

HSP 1 Score: 573.9 bits (1478), Expect = 1.8e-162
Identity = 279/461 (60.52%), Postives = 352/461 (76.36%), Query Frame = 0


Query: 75  LVDHELSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGK 134
           +++H L E+DP+V  I+ KE QRQ +S+ LIASEN TSRAV +A+GS ++NKYSEGLPG 
Sbjct: 13  MLEHSLVESDPQVAEIMKKEVQRQRESIILIASENVTSRAVFDALGSPMSNKYSEGLPGA 72

Query: 135 RYYGGNEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPANFEVYTAVLSPHDRIMG 194
           RYYGGN+HIDE+E LCQ RAL AFHLD  +WGVNVQ LSGSPAN +VY A++  H R+MG
Sbjct: 73  RYYGGNQHIDEIEVLCQNRALEAFHLDPKQWGVNVQCLSGSPANLQVYQAIMPVHGRLMG 132

Query: 195 LDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYDMLEKTANLFRPKLIIA 254
           LDLPHGGHLSHG+ TP+R++S  S YFE+MPYR++  TG++DYD LEK A LFRPK+++A
Sbjct: 133 LDLPHGGHLSHGYQTPQRKISAVSTYFETMPYRVNIDTGLIDYDTLEKNAQLFRPKVLVA 192

Query: 255 GASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADPFEYCDIVTTTTHKSLR 314
           G SAY R  DY RMRKIAD+VGA+L++DMAHISGL+A+ V+  PF Y D+VTTTTHKSLR
Sbjct: 193 GTSAYCRLIDYERMRKIADSVGAYLVVDMAHISGLIASEVIPSPFLYADVVTTTTHKSLR 252

Query: 315 GPRGGMIFFRK-----DPILG----VDLESAINNAVFPGLQGGPHNHTIGGLAVCLKHAQ 374
           GPRG MIFFR+     D   G     DLE  IN +VFPG QGGPHNHTI  LAV LK A 
Sbjct: 253 GPRGAMIFFRRGVRSVDAKTGKETLYDLEDKINFSVFPGHQGGPHNHTITALAVALKQAA 312

Query: 375 SPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEKILDMA 434
           SPEF+ YQ KVV+N +AL  +L ELGYKLVS G+D+H+VLVDLRP+G+DGARVE +L+  
Sbjct: 313 SPEFKEYQQKVVANAKALEKKLKELGYKLVSDGTDSHMVLVDLRPIGVDGARVEFLLEQI 372

Query: 435 SITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQITLNAKQLAP 494
           +IT NKN+VPGDKSAL PGG+RIG+PAMT+RGF E +F  VA F+ E V++    +   P
Sbjct: 373 NITCNKNAVPGDKSALTPGGLRIGTPAMTSRGFGEADFEKVAVFVDEAVKLCKEIQASLP 432

Query: 495 --GSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIP 525
              +K ++F   + T+D P   R++EL+  + A +  FP+P
Sbjct: 433 KEANKQKDFKAKIATSDIP---RINELKQEIAAWSNTFPLP 470

[Close]

BLAST of MC10g0817 vs. ExPASy Swiss-Prot
Match: P50433 (Serine hydroxymethyltransferase, mitochondrial OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 5.3e-162
Identity = 278/454 (61.23%), Postives = 343/454 (75.55%), Query Frame = 0


Query: 80  LSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGG 139
           L   DPE+  II  EK RQ+K LELI SENFTS +VM+AVGS +TNKYSEG PG RYYGG
Sbjct: 56  LEVVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGG 115

Query: 140 NEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPANFEVYTAVLSPHDRIMGLDLPH 199
           NE+ID  ETLCQ+RAL AF LD  KWGVNVQPLSGSPANF+VYTA+L PH+RIM LDLPH
Sbjct: 116 NEYIDMAETLCQKRALEAFRLDPAKWGVNVQPLSGSPANFQVYTALLKPHERIMALDLPH 175

Query: 200 GGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYDMLEKTANLFRPKLIIAGASAY 259
           GGHLSHG+ T  +++S  SI+FE+MPYRLDE+TG +DYD LEK+A LFRPKLI+AGASAY
Sbjct: 176 GGHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLEKSATLFRPKLIVAGASAY 235

Query: 260 PRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADPFEYCDIVTTTTHKSLRGPRGG 319
            R +DY R+RK+ +   A L+ DMAHISGLVAA V+  PF+Y D+VTTTTHKSLRGPRG 
Sbjct: 236 ARLYDYDRIRKVCNKQKAILLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGA 295

Query: 320 MIFFRK--------DPILGVDLESAINNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFRV 379
           MIF+RK           +  D E  IN AVFPGLQGGPHNHTI GLAV LK A +PE+R 
Sbjct: 296 MIFYRKGVKEVNKQGKEVFYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYRA 355

Query: 380 YQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEKILDMASITLNK 439
           YQ +V+SN    A  L E GY+LVSGG+DNHLVLV+++  GIDG+RVEK+L+   I  NK
Sbjct: 356 YQEQVLSNSSKFAQALGEKGYELVSGGTDNHLVLVNMKNKGIDGSRVEKVLEAVHIAANK 415

Query: 440 NSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQITLNAKQLAPGSKLQE 499
           N+VPGD SA+VPGGIR+G+PA+T+RGF E++F+ VADF    V+I +  K    G+KL++
Sbjct: 416 NTVPGDVSAMVPGGIRMGTPALTSRGFLEEDFVKVADFFDAAVKIAVKVKAETQGTKLKD 475

Query: 500 FLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPG 526
           F+  ++++  P+   +++LR  VE    QFP  G
Sbjct: 476 FVATLESS-APIKSEIAKLRHDVEEYAKQFPTIG 508

[Close]

BLAST of MC10g0817 vs. NCBI nr
Match: XP_022136789.1 (serine hydroxymethyltransferase 3, chloroplastic [Momordica charantia])

HSP 1 Score: 1046 bits (2706), Expect = 0.0
Identity = 526/526 (100.00%), Postives = 526/526 (100.00%), Query Frame = 0


Query: 1   MQAASGASMMGSLQVVVCAKGSAFPSKGPSICVFPQPKKLNILKPCKSSNVEASAVAGRS 60
           MQAASGASMMGSLQVVVCAKGSAFPSKGPSICVFPQPKKLNILKPCKSSNVEASAVAGRS
Sbjct: 1   MQAASGASMMGSLQVVVCAKGSAFPSKGPSICVFPQPKKLNILKPCKSSNVEASAVAGRS 60

Query: 61  SVSVTVPEIGGGSHLVDHELSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVG 120
           SVSVTVPEIGGGSHLVDHELSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVG
Sbjct: 61  SVSVTVPEIGGGSHLVDHELSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVG 120

Query: 121 SCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPANFE 180
           SCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPANFE
Sbjct: 121 SCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPANFE 180

Query: 181 VYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYDML 240
           VYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYDML
Sbjct: 181 VYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYDML 240

Query: 241 EKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADPFE 300
           EKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADPFE
Sbjct: 241 EKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADPFE 300

Query: 301 YCDIVTTTTHKSLRGPRGGMIFFRKDPILGVDLESAINNAVFPGLQGGPHNHTIGGLAVC 360
           YCDIVTTTTHKSLRGPRGGMIFFRKDPILGVDLESAINNAVFPGLQGGPHNHTIGGLAVC
Sbjct: 301 YCDIVTTTTHKSLRGPRGGMIFFRKDPILGVDLESAINNAVFPGLQGGPHNHTIGGLAVC 360

Query: 361 LKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEK 420
           LKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEK
Sbjct: 361 LKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEK 420

Query: 421 ILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQITLNA 480
           ILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQITLNA
Sbjct: 421 ILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQITLNA 480

Query: 481 KQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPGL 526
           KQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPGL
Sbjct: 481 KQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPGL 526

[Close]

BLAST of MC10g0817 vs. NCBI nr
Match: XP_011653260.1 (serine hydroxymethyltransferase 3, chloroplastic [Cucumis sativus] >XP_031740593.1 serine hydroxymethyltransferase 3, chloroplastic [Cucumis sativus] >KGN53502.1 hypothetical protein Csa_014386 [Cucumis sativus])

HSP 1 Score: 982 bits (2538), Expect = 0.0
Identity = 490/528 (92.80%), Postives = 508/528 (96.21%), Query Frame = 0


Query: 1   MQAASGASMMGSLQVVVCAKGSAFPSKGPSICVFPQPKKLNILKPCKSSNVEASAVAGR- 60
           MQA SG ++MGSLQV VC KGS FPSK  SICVFPQ KK+NILKPCKS  VEAS VAG+ 
Sbjct: 1   MQATSGVAVMGSLQVAVCGKGSCFPSKSSSICVFPQQKKMNILKPCKSFKVEASMVAGKP 60

Query: 61  -SSVSVTVPEIGGGSHLVDHELSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEA 120
            SSVSVTVPEIGG S+ VDH LSETDPEVRSII+KEKQRQFKSLELIASENFTSRAVMEA
Sbjct: 61  SSSVSVTVPEIGGVSNFVDHALSETDPEVRSIIDKEKQRQFKSLELIASENFTSRAVMEA 120

Query: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPAN 180
           VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLD NKWGVNVQPLSGSPAN
Sbjct: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDNNKWGVNVQPLSGSPAN 180

Query: 181 FEVYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240
           FEVYTAVL+PHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD
Sbjct: 181 FEVYTAVLNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240

Query: 241 MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300
           MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP
Sbjct: 241 MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300

Query: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPILGVDLESAINNAVFPGLQGGPHNHTIGGLA 360
           FEYCDIVTTTTHKSLRGPRGGMIFFRKDP+LGVDLESAINNAVFPGLQGGPHNHTIGGLA
Sbjct: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360

Query: 361 VCLKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420
           VCLKHAQSPEF+VYQNKV++NCRALA+RLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV
Sbjct: 361 VCLKHAQSPEFKVYQNKVIANCRALANRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420

Query: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQITL 480
           EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEF+AVADFIHEGV+ITL
Sbjct: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFVAVADFIHEGVKITL 480

Query: 481 NAKQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPGL 526
           +AK LAPGSKLQEFLKFV T+DFPL DR+S+LRSRVEALTTQFPIPGL
Sbjct: 481 DAKPLAPGSKLQEFLKFVTTSDFPLTDRISDLRSRVEALTTQFPIPGL 528

[Close]

BLAST of MC10g0817 vs. NCBI nr
Match: XP_022942709.1 (serine hydroxymethyltransferase 3, chloroplastic-like [Cucurbita moschata] >XP_022942710.1 serine hydroxymethyltransferase 3, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 979 bits (2531), Expect = 0.0
Identity = 490/528 (92.80%), Postives = 510/528 (96.59%), Query Frame = 0


Query: 1   MQAASGASMMGSLQVVVCAKGSAFPSKGPSICVFPQPKKLNILKPCKSSNVEASAVAGR- 60
           MQAASGA++MGSLQVVVCAKGS+FPSKG SICVFPQ KK  +LKPCKSS+VEA+ VAG+ 
Sbjct: 1   MQAASGAAVMGSLQVVVCAKGSSFPSKGSSICVFPQHKKWTVLKPCKSSSVEANMVAGKA 60

Query: 61  -SSVSVTVPEIGGGSHLVDHELSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEA 120
            SSVSV+ P++GGGS  VDH LSE DP+VRSII+KEKQRQFKSLELIASENFTSRAVMEA
Sbjct: 61  SSSVSVSAPQLGGGSGFVDHGLSEADPDVRSIIDKEKQRQFKSLELIASENFTSRAVMEA 120

Query: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPAN 180
           VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAF LD NKWGVNVQPLSGSPAN
Sbjct: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFRLDNNKWGVNVQPLSGSPAN 180

Query: 181 FEVYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240
           FEVYTA+L+PHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD
Sbjct: 181 FEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240

Query: 241 MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300
           MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP
Sbjct: 241 MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300

Query: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPILGVDLESAINNAVFPGLQGGPHNHTIGGLA 360
           FEYCDIVTTTTHKSLRGPRGGMIFFRKDP+LGVDLESAINNAVFPGLQGGPHNHTIGGLA
Sbjct: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360

Query: 361 VCLKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420
           VCLKHAQSPEF+ YQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV
Sbjct: 361 VCLKHAQSPEFKDYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420

Query: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQITL 480
           EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGV+ITL
Sbjct: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVKITL 480

Query: 481 NAKQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPGL 526
           +AK LAPGSKLQEFLKFV T+DFPL+DRVS LRSRVEALTTQFPIPGL
Sbjct: 481 DAKPLAPGSKLQEFLKFVATSDFPLIDRVSNLRSRVEALTTQFPIPGL 528

[Close]

BLAST of MC10g0817 vs. NCBI nr
Match: XP_022980294.1 (serine hydroxymethyltransferase 3, chloroplastic-like [Cucurbita maxima] >XP_022980300.1 serine hydroxymethyltransferase 3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 979 bits (2530), Expect = 0.0
Identity = 490/528 (92.80%), Postives = 512/528 (96.97%), Query Frame = 0


Query: 1   MQAASGASMMGSLQVVVCAKGSAFPSKGPSICVFPQPKKLNILKPCKSSNVEASAVAGR- 60
           MQAASGA++MGSLQVVVCAKGS+FPSKG SICVFPQ KK  +LKPCKSS+VEA+ VAG+ 
Sbjct: 1   MQAASGAAVMGSLQVVVCAKGSSFPSKGSSICVFPQHKKWIVLKPCKSSSVEANMVAGKA 60

Query: 61  -SSVSVTVPEIGGGSHLVDHELSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEA 120
            SSVSV+ P++GGGS  VDH LSE DP+VRSII+KEKQRQFKSLELIASENFTSRAVMEA
Sbjct: 61  SSSVSVSSPQLGGGSGFVDHGLSEADPDVRSIIDKEKQRQFKSLELIASENFTSRAVMEA 120

Query: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPAN 180
           VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAF LD+NKWGVNVQPLSGSPAN
Sbjct: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFRLDKNKWGVNVQPLSGSPAN 180

Query: 181 FEVYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240
           FEVYTA+L+PHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD
Sbjct: 181 FEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240

Query: 241 MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300
           MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP
Sbjct: 241 MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300

Query: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPILGVDLESAINNAVFPGLQGGPHNHTIGGLA 360
           FE+CDIVTTTTHKSLRGPRGGMIFFRKDP+LGVDLESAINNAVFPGLQGGPHNHTIGGLA
Sbjct: 301 FEFCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360

Query: 361 VCLKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420
           VCLKHAQSPEF+ YQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV
Sbjct: 361 VCLKHAQSPEFKDYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420

Query: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQITL 480
           EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGV+ITL
Sbjct: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVKITL 480

Query: 481 NAKQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPGL 526
           NAK LAPGSKLQEFLKFV T+DFPL+DRVS+LRSRVEALTTQFPIPGL
Sbjct: 481 NAKPLAPGSKLQEFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPGL 528

[Close]

BLAST of MC10g0817 vs. NCBI nr
Match: XP_008451853.1 (PREDICTED: serine hydroxymethyltransferase 3, chloroplastic [Cucumis melo] >TYK16426.1 serine hydroxymethyltransferase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 977 bits (2526), Expect = 0.0
Identity = 489/528 (92.61%), Postives = 506/528 (95.83%), Query Frame = 0


Query: 1   MQAASGASMMGSLQVVVCAKGSAFPSKGPSICVFPQPKKLNILKPCKSSNVEASAVAGR- 60
           MQA SGA++MGSL+V VC KGS FPSK  SICVFPQ KK+NILKP KSS V+AS V G+ 
Sbjct: 1   MQATSGAAVMGSLEVAVCGKGSCFPSKSSSICVFPQHKKMNILKPSKSSKVKASMVVGKP 60

Query: 61  -SSVSVTVPEIGGGSHLVDHELSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEA 120
            SS SVTVPEIGG SH VDH LSETDPEVRSII+KEKQRQFKSLELIASENFTSRAVMEA
Sbjct: 61  SSSASVTVPEIGGVSHFVDHALSETDPEVRSIIDKEKQRQFKSLELIASENFTSRAVMEA 120

Query: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPAN 180
           VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLD NKWGVNVQPLSGSPAN
Sbjct: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDNNKWGVNVQPLSGSPAN 180

Query: 181 FEVYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240
           FEVYTAVL+PHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD
Sbjct: 181 FEVYTAVLNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240

Query: 241 MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300
           MLE+TANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP
Sbjct: 241 MLERTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300

Query: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPILGVDLESAINNAVFPGLQGGPHNHTIGGLA 360
           FEYCDIVTTTTHKSLRGPRGGMIFFRKDP+LGVDLESAINNAVFPGLQGGPHNHTIGGLA
Sbjct: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360

Query: 361 VCLKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420
           VCLKHAQSPEF+VYQNKV++NCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV
Sbjct: 361 VCLKHAQSPEFKVYQNKVIANCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420

Query: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQITL 480
           EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEF AVADFIHEGV+ITL
Sbjct: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFAAVADFIHEGVKITL 480

Query: 481 NAKQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPGL 526
           +AK LAPGSKLQEFLKFV T+DFPL DRVS+LRSRVEALTTQFPIPGL
Sbjct: 481 DAKPLAPGSKLQEFLKFVTTSDFPLTDRVSDLRSRVEALTTQFPIPGL 528

[Close]

BLAST of MC10g0817 vs. ExPASy TrEMBL
Match: A0A6J1C5C3 (Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111008402 PE=3 SV=1)

HSP 1 Score: 1046 bits (2706), Expect = 0.0
Identity = 526/526 (100.00%), Postives = 526/526 (100.00%), Query Frame = 0


Query: 1   MQAASGASMMGSLQVVVCAKGSAFPSKGPSICVFPQPKKLNILKPCKSSNVEASAVAGRS 60
           MQAASGASMMGSLQVVVCAKGSAFPSKGPSICVFPQPKKLNILKPCKSSNVEASAVAGRS
Sbjct: 1   MQAASGASMMGSLQVVVCAKGSAFPSKGPSICVFPQPKKLNILKPCKSSNVEASAVAGRS 60

Query: 61  SVSVTVPEIGGGSHLVDHELSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVG 120
           SVSVTVPEIGGGSHLVDHELSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVG
Sbjct: 61  SVSVTVPEIGGGSHLVDHELSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVG 120

Query: 121 SCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPANFE 180
           SCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPANFE
Sbjct: 121 SCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPANFE 180

Query: 181 VYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYDML 240
           VYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYDML
Sbjct: 181 VYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYDML 240

Query: 241 EKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADPFE 300
           EKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADPFE
Sbjct: 241 EKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADPFE 300

Query: 301 YCDIVTTTTHKSLRGPRGGMIFFRKDPILGVDLESAINNAVFPGLQGGPHNHTIGGLAVC 360
           YCDIVTTTTHKSLRGPRGGMIFFRKDPILGVDLESAINNAVFPGLQGGPHNHTIGGLAVC
Sbjct: 301 YCDIVTTTTHKSLRGPRGGMIFFRKDPILGVDLESAINNAVFPGLQGGPHNHTIGGLAVC 360

Query: 361 LKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEK 420
           LKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEK
Sbjct: 361 LKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEK 420

Query: 421 ILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQITLNA 480
           ILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQITLNA
Sbjct: 421 ILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQITLNA 480

Query: 481 KQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPGL 526
           KQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPGL
Sbjct: 481 KQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPGL 526

[Close]

BLAST of MC10g0817 vs. ExPASy TrEMBL
Match: A0A0A0KYS6 (Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_4G062380 PE=3 SV=1)

HSP 1 Score: 982 bits (2538), Expect = 0.0
Identity = 490/528 (92.80%), Postives = 508/528 (96.21%), Query Frame = 0


Query: 1   MQAASGASMMGSLQVVVCAKGSAFPSKGPSICVFPQPKKLNILKPCKSSNVEASAVAGR- 60
           MQA SG ++MGSLQV VC KGS FPSK  SICVFPQ KK+NILKPCKS  VEAS VAG+ 
Sbjct: 1   MQATSGVAVMGSLQVAVCGKGSCFPSKSSSICVFPQQKKMNILKPCKSFKVEASMVAGKP 60

Query: 61  -SSVSVTVPEIGGGSHLVDHELSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEA 120
            SSVSVTVPEIGG S+ VDH LSETDPEVRSII+KEKQRQFKSLELIASENFTSRAVMEA
Sbjct: 61  SSSVSVTVPEIGGVSNFVDHALSETDPEVRSIIDKEKQRQFKSLELIASENFTSRAVMEA 120

Query: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPAN 180
           VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLD NKWGVNVQPLSGSPAN
Sbjct: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDNNKWGVNVQPLSGSPAN 180

Query: 181 FEVYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240
           FEVYTAVL+PHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD
Sbjct: 181 FEVYTAVLNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240

Query: 241 MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300
           MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP
Sbjct: 241 MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300

Query: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPILGVDLESAINNAVFPGLQGGPHNHTIGGLA 360
           FEYCDIVTTTTHKSLRGPRGGMIFFRKDP+LGVDLESAINNAVFPGLQGGPHNHTIGGLA
Sbjct: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360

Query: 361 VCLKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420
           VCLKHAQSPEF+VYQNKV++NCRALA+RLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV
Sbjct: 361 VCLKHAQSPEFKVYQNKVIANCRALANRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420

Query: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQITL 480
           EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEF+AVADFIHEGV+ITL
Sbjct: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFVAVADFIHEGVKITL 480

Query: 481 NAKQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPGL 526
           +AK LAPGSKLQEFLKFV T+DFPL DR+S+LRSRVEALTTQFPIPGL
Sbjct: 481 DAKPLAPGSKLQEFLKFVTTSDFPLTDRISDLRSRVEALTTQFPIPGL 528

[Close]

BLAST of MC10g0817 vs. ExPASy TrEMBL
Match: A0A6J1FS47 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447665 PE=3 SV=1)

HSP 1 Score: 979 bits (2531), Expect = 0.0
Identity = 490/528 (92.80%), Postives = 510/528 (96.59%), Query Frame = 0


Query: 1   MQAASGASMMGSLQVVVCAKGSAFPSKGPSICVFPQPKKLNILKPCKSSNVEASAVAGR- 60
           MQAASGA++MGSLQVVVCAKGS+FPSKG SICVFPQ KK  +LKPCKSS+VEA+ VAG+ 
Sbjct: 1   MQAASGAAVMGSLQVVVCAKGSSFPSKGSSICVFPQHKKWTVLKPCKSSSVEANMVAGKA 60

Query: 61  -SSVSVTVPEIGGGSHLVDHELSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEA 120
            SSVSV+ P++GGGS  VDH LSE DP+VRSII+KEKQRQFKSLELIASENFTSRAVMEA
Sbjct: 61  SSSVSVSAPQLGGGSGFVDHGLSEADPDVRSIIDKEKQRQFKSLELIASENFTSRAVMEA 120

Query: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPAN 180
           VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAF LD NKWGVNVQPLSGSPAN
Sbjct: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFRLDNNKWGVNVQPLSGSPAN 180

Query: 181 FEVYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240
           FEVYTA+L+PHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD
Sbjct: 181 FEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240

Query: 241 MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300
           MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP
Sbjct: 241 MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300

Query: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPILGVDLESAINNAVFPGLQGGPHNHTIGGLA 360
           FEYCDIVTTTTHKSLRGPRGGMIFFRKDP+LGVDLESAINNAVFPGLQGGPHNHTIGGLA
Sbjct: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360

Query: 361 VCLKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420
           VCLKHAQSPEF+ YQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV
Sbjct: 361 VCLKHAQSPEFKDYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420

Query: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQITL 480
           EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGV+ITL
Sbjct: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVKITL 480

Query: 481 NAKQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPGL 526
           +AK LAPGSKLQEFLKFV T+DFPL+DRVS LRSRVEALTTQFPIPGL
Sbjct: 481 DAKPLAPGSKLQEFLKFVATSDFPLIDRVSNLRSRVEALTTQFPIPGL 528

[Close]

BLAST of MC10g0817 vs. ExPASy TrEMBL
Match: A0A6J1IVW3 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111479708 PE=3 SV=1)

HSP 1 Score: 979 bits (2530), Expect = 0.0
Identity = 490/528 (92.80%), Postives = 512/528 (96.97%), Query Frame = 0


Query: 1   MQAASGASMMGSLQVVVCAKGSAFPSKGPSICVFPQPKKLNILKPCKSSNVEASAVAGR- 60
           MQAASGA++MGSLQVVVCAKGS+FPSKG SICVFPQ KK  +LKPCKSS+VEA+ VAG+ 
Sbjct: 1   MQAASGAAVMGSLQVVVCAKGSSFPSKGSSICVFPQHKKWIVLKPCKSSSVEANMVAGKA 60

Query: 61  -SSVSVTVPEIGGGSHLVDHELSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEA 120
            SSVSV+ P++GGGS  VDH LSE DP+VRSII+KEKQRQFKSLELIASENFTSRAVMEA
Sbjct: 61  SSSVSVSSPQLGGGSGFVDHGLSEADPDVRSIIDKEKQRQFKSLELIASENFTSRAVMEA 120

Query: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPAN 180
           VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAF LD+NKWGVNVQPLSGSPAN
Sbjct: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFRLDKNKWGVNVQPLSGSPAN 180

Query: 181 FEVYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240
           FEVYTA+L+PHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD
Sbjct: 181 FEVYTAILNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240

Query: 241 MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300
           MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP
Sbjct: 241 MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300

Query: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPILGVDLESAINNAVFPGLQGGPHNHTIGGLA 360
           FE+CDIVTTTTHKSLRGPRGGMIFFRKDP+LGVDLESAINNAVFPGLQGGPHNHTIGGLA
Sbjct: 301 FEFCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360

Query: 361 VCLKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420
           VCLKHAQSPEF+ YQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV
Sbjct: 361 VCLKHAQSPEFKDYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420

Query: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQITL 480
           EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGV+ITL
Sbjct: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVKITL 480

Query: 481 NAKQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPGL 526
           NAK LAPGSKLQEFLKFV T+DFPL+DRVS+LRSRVEALTTQFPIPGL
Sbjct: 481 NAKPLAPGSKLQEFLKFVTTSDFPLIDRVSDLRSRVEALTTQFPIPGL 528

[Close]

BLAST of MC10g0817 vs. ExPASy TrEMBL
Match: A0A5D3D173 (Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G002230 PE=3 SV=1)

HSP 1 Score: 977 bits (2526), Expect = 0.0
Identity = 489/528 (92.61%), Postives = 506/528 (95.83%), Query Frame = 0


Query: 1   MQAASGASMMGSLQVVVCAKGSAFPSKGPSICVFPQPKKLNILKPCKSSNVEASAVAGR- 60
           MQA SGA++MGSL+V VC KGS FPSK  SICVFPQ KK+NILKP KSS V+AS V G+ 
Sbjct: 1   MQATSGAAVMGSLEVAVCGKGSCFPSKSSSICVFPQHKKMNILKPSKSSKVKASMVVGKP 60

Query: 61  -SSVSVTVPEIGGGSHLVDHELSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEA 120
            SS SVTVPEIGG SH VDH LSETDPEVRSII+KEKQRQFKSLELIASENFTSRAVMEA
Sbjct: 61  SSSASVTVPEIGGVSHFVDHALSETDPEVRSIIDKEKQRQFKSLELIASENFTSRAVMEA 120

Query: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPAN 180
           VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLD NKWGVNVQPLSGSPAN
Sbjct: 121 VGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDNNKWGVNVQPLSGSPAN 180

Query: 181 FEVYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240
           FEVYTAVL+PHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD
Sbjct: 181 FEVYTAVLNPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYD 240

Query: 241 MLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300
           MLE+TANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP
Sbjct: 241 MLERTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADP 300

Query: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPILGVDLESAINNAVFPGLQGGPHNHTIGGLA 360
           FEYCDIVTTTTHKSLRGPRGGMIFFRKDP+LGVDLESAINNAVFPGLQGGPHNHTIGGLA
Sbjct: 301 FEYCDIVTTTTHKSLRGPRGGMIFFRKDPVLGVDLESAINNAVFPGLQGGPHNHTIGGLA 360

Query: 361 VCLKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420
           VCLKHAQSPEF+VYQNKV++NCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV
Sbjct: 361 VCLKHAQSPEFKVYQNKVIANCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARV 420

Query: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQITL 480
           EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEF AVADFIHEGV+ITL
Sbjct: 421 EKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFAAVADFIHEGVKITL 480

Query: 481 NAKQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPGL 526
           +AK LAPGSKLQEFLKFV T+DFPL DRVS+LRSRVEALTTQFPIPGL
Sbjct: 481 DAKPLAPGSKLQEFLKFVTTSDFPLTDRVSDLRSRVEALTTQFPIPGL 528

[Close]

BLAST of MC10g0817 vs. TAIR 10
Match: AT4G32520.1 (serine hydroxymethyltransferase 3 )

HSP 1 Score: 839.0 bits (2166), Expect = 2.2e-243
Identity = 418/529 (79.02%), Postives = 460/529 (86.96%), Query Frame = 0


Query: 1   MQAASGASMMGSLQVVVCAKGSAFPSKGPSICVFPQPKKLNILKPCKSSNVEASAVAGRS 60
           MQA  G + M SLQ     +GS FP   P +  F Q  K NI KP +SS ++ + V+   
Sbjct: 1   MQACCGGNSMASLQQPGRVQGSVFPPIMPPVTKFSQQLKFNISKPFRSSFLKRNLVSEMR 60

Query: 61  SVSVTVPEIGGGSHLV---DHELSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVME 120
           + SV++P +   S  +   D+ L E DPEVR+II KEK RQF+SLELIASENFTSRAVME
Sbjct: 61  ASSVSLPNVEISSKEIPFEDYGLGEVDPEVRTIITKEKDRQFRSLELIASENFTSRAVME 120

Query: 121 AVGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPA 180
           AVGSCLTNKYSEGLPGKRYYGGNE+ID+LETLCQ RALAAF LD  KWGVNVQPLSGSPA
Sbjct: 121 AVGSCLTNKYSEGLPGKRYYGGNEYIDQLETLCQNRALAAFRLDSTKWGVNVQPLSGSPA 180

Query: 181 NFEVYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDY 240
           NF VYTA+LSPHDRIMGLDLPHGGHLSHGFMT KRRVSGTSIYFESMPYRLDE+TGIVDY
Sbjct: 181 NFAVYTAILSPHDRIMGLDLPHGGHLSHGFMTAKRRVSGTSIYFESMPYRLDESTGIVDY 240

Query: 241 DMLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVAD 300
           DMLEKTA LFRPKLIIAGASAY RDFDYPRMRKIAD+VGAFLMMDMAHISGLVAASVVAD
Sbjct: 241 DMLEKTATLFRPKLIIAGASAYSRDFDYPRMRKIADSVGAFLMMDMAHISGLVAASVVAD 300

Query: 301 PFEYCDIVTTTTHKSLRGPRGGMIFFRKDPILGVDLESAINNAVFPGLQGGPHNHTIGGL 360
           PFEYCDIVTTTTHKSLRGPRGGMIFFRKDPI GVDLESA+NNAVFPGLQGGPHNHTIGGL
Sbjct: 301 PFEYCDIVTTTTHKSLRGPRGGMIFFRKDPINGVDLESAVNNAVFPGLQGGPHNHTIGGL 360

Query: 361 AVCLKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGAR 420
           AVCLKHAQSPEF+ YQ +VVSNCRALA+RLVELG+KLVSGGSDNHLVLVDLRP+G+DGAR
Sbjct: 361 AVCLKHAQSPEFKAYQKRVVSNCRALANRLVELGFKLVSGGSDNHLVLVDLRPMGMDGAR 420

Query: 421 VEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQIT 480
           VEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRG +EK+F+ VADFI EGV+IT
Sbjct: 421 VEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGLSEKDFVVVADFIKEGVEIT 480

Query: 481 LNAKQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPGL 527
           + AK+ APGSKLQ+F KFV + +FPL +RV  L+ RVE  T++FPIPG+
Sbjct: 481 MEAKKAAPGSKLQDFNKFVTSPEFPLKERVKSLKERVETFTSRFPIPGV 529

[Close]

BLAST of MC10g0817 vs. TAIR 10
Match: AT4G32520.2 (serine hydroxymethyltransferase 3 )

HSP 1 Score: 839.0 bits (2166), Expect = 2.2e-243
Identity = 418/529 (79.02%), Postives = 460/529 (86.96%), Query Frame = 0


Query: 1   MQAASGASMMGSLQVVVCAKGSAFPSKGPSICVFPQPKKLNILKPCKSSNVEASAVAGRS 60
           MQA  G + M SLQ     +GS FP   P +  F Q  K NI KP +SS ++ + V+   
Sbjct: 1   MQACCGGNSMASLQQPGRVQGSVFPPIMPPVTKFSQQLKFNISKPFRSSFLKRNLVSEMR 60

Query: 61  SVSVTVPEIGGGSHLV---DHELSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVME 120
           + SV++P +   S  +   D+ L E DPEVR+II KEK RQF+SLELIASENFTSRAVME
Sbjct: 61  ASSVSLPNVEISSKEIPFEDYGLGEVDPEVRTIITKEKDRQFRSLELIASENFTSRAVME 120

Query: 121 AVGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPA 180
           AVGSCLTNKYSEGLPGKRYYGGNE+ID+LETLCQ RALAAF LD  KWGVNVQPLSGSPA
Sbjct: 121 AVGSCLTNKYSEGLPGKRYYGGNEYIDQLETLCQNRALAAFRLDSTKWGVNVQPLSGSPA 180

Query: 181 NFEVYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDY 240
           NF VYTA+LSPHDRIMGLDLPHGGHLSHGFMT KRRVSGTSIYFESMPYRLDE+TGIVDY
Sbjct: 181 NFAVYTAILSPHDRIMGLDLPHGGHLSHGFMTAKRRVSGTSIYFESMPYRLDESTGIVDY 240

Query: 241 DMLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVAD 300
           DMLEKTA LFRPKLIIAGASAY RDFDYPRMRKIAD+VGAFLMMDMAHISGLVAASVVAD
Sbjct: 241 DMLEKTATLFRPKLIIAGASAYSRDFDYPRMRKIADSVGAFLMMDMAHISGLVAASVVAD 300

Query: 301 PFEYCDIVTTTTHKSLRGPRGGMIFFRKDPILGVDLESAINNAVFPGLQGGPHNHTIGGL 360
           PFEYCDIVTTTTHKSLRGPRGGMIFFRKDPI GVDLESA+NNAVFPGLQGGPHNHTIGGL
Sbjct: 301 PFEYCDIVTTTTHKSLRGPRGGMIFFRKDPINGVDLESAVNNAVFPGLQGGPHNHTIGGL 360

Query: 361 AVCLKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGAR 420
           AVCLKHAQSPEF+ YQ +VVSNCRALA+RLVELG+KLVSGGSDNHLVLVDLRP+G+DGAR
Sbjct: 361 AVCLKHAQSPEFKAYQKRVVSNCRALANRLVELGFKLVSGGSDNHLVLVDLRPMGMDGAR 420

Query: 421 VEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQIT 480
           VEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRG +EK+F+ VADFI EGV+IT
Sbjct: 421 VEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGLSEKDFVVVADFIKEGVEIT 480

Query: 481 LNAKQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPGL 527
           + AK+ APGSKLQ+F KFV + +FPL +RV  L+ RVE  T++FPIPG+
Sbjct: 481 MEAKKAAPGSKLQDFNKFVTSPEFPLKERVKSLKERVETFTSRFPIPGV 529

[Close]

BLAST of MC10g0817 vs. TAIR 10
Match: AT4G37930.1 (serine transhydroxymethyltransferase 1 )

HSP 1 Score: 576.2 bits (1484), Expect = 2.6e-164
Identity = 284/488 (58.20%), Postives = 358/488 (73.36%), Query Frame = 0


Query: 46  CKSSNVEASAVAGRSSVSVTVPEIGGGSHLVDHELSETDPEVRSIINKEKQRQFKSLELI 105
           C  S++ + AV  +    VT P+       ++  L E DPE+  II  EK RQ+K LELI
Sbjct: 27  CYMSSLPSEAVDEKERSRVTWPK------QLNAPLEEVDPEIADIIEHEKARQWKGLELI 86

Query: 106 ASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEHIDELETLCQQRALAAFHLDENKW 165
            SENFTS +VM+AVGS +TNKYSEG PG RYYGGNE+ID  ETLCQ+RAL AF LD  KW
Sbjct: 87  PSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAETLCQKRALEAFRLDPEKW 146

Query: 166 GVNVQPLSGSPANFEVYTAVLSPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMP 225
           GVNVQPLSGSPANF VYTA+L PH+RIM LDLPHGGHLSHG+ T  +++S  SI+FE+MP
Sbjct: 147 GVNVQPLSGSPANFHVYTALLKPHERIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMP 206

Query: 226 YRLDETTGIVDYDMLEKTANLFRPKLIIAGASAYPRDFDYPRMRKIADAVGAFLMMDMAH 285
           YRLDE+TG +DYD +EK+A LFRPKLI+AGASAY R +DY R+RK+ +   A ++ DMAH
Sbjct: 207 YRLDESTGYIDYDQMEKSATLFRPKLIVAGASAYARLYDYARIRKVCNKQKAVMLADMAH 266

Query: 286 ISGLVAASVVADPFEYCDIVTTTTHKSLRGPRGGMIFFRK--------DPILGVDLESAI 345
           ISGLVAA+V+  PF+Y D+VTTTTHKSLRGPRG MIFFRK           +  D E  I
Sbjct: 267 ISGLVAANVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKGVKEINKQGKEVLYDFEDKI 326

Query: 346 NNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFRVYQNKVVSNCRALASRLVELGYKLVSG 405
           N AVFPGLQGGPHNHTI GLAV LK A + E++ YQ +V+SN    A  L+E GY+LVSG
Sbjct: 327 NQAVFPGLQGGPHNHTITGLAVALKQATTSEYKAYQEQVLSNSAKFAQTLMERGYELVSG 386

Query: 406 GSDNHLVLVDLRPLGIDGARVEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRG 465
           G+DNHLVLV+L+P GIDG+RVEK+L+   I  NKN+VPGD SA+VPGGIR+G+PA+T+RG
Sbjct: 387 GTDNHLVLVNLKPKGIDGSRVEKVLEAVHIASNKNTVPGDVSAMVPGGIRMGTPALTSRG 446

Query: 466 FTEKEFIAVADFIHEGVQITLNAKQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEAL 525
           F E++F  VA++  + V I L  K  A G+KL++F+  ++++   +   +++LR  VE  
Sbjct: 447 FVEEDFAKVAEYFDKAVTIALKVKSEAQGTKLKDFVSAMESSS-TIQSEIAKLRHEVEEF 506

[Close]

BLAST of MC10g0817 vs. TAIR 10
Match: AT5G26780.1 (serine hydroxymethyltransferase 2 )

HSP 1 Score: 570.1 bits (1468), Expect = 1.9e-162
Identity = 278/454 (61.23%), Postives = 343/454 (75.55%), Query Frame = 0


Query: 80  LSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGG 139
           L E DPEV  II  EK RQ+K  ELI SENFTS +VM+AVGS +TNKYSEG PG RYYGG
Sbjct: 55  LDEIDPEVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGG 114

Query: 140 NEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPANFEVYTAVLSPHDRIMGLDLPH 199
           NE+ID  ETLCQ+RAL AF LD +KWGVNVQ LSGSPANF+VYTA+L PH+RIM LDLPH
Sbjct: 115 NEYIDMAETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLPH 174

Query: 200 GGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYDMLEKTANLFRPKLIIAGASAY 259
           GGHLSHG+ T  +++S  SI+FE+MPYRLDE TG +DYD LEK+A LFRPKLI+AGASAY
Sbjct: 175 GGHLSHGYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASAY 234

Query: 260 PRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADPFEYCDIVTTTTHKSLRGPRGG 319
            R +DY R+RK+ +   A ++ DMAHISGLVAA V+  PFEY D+VTTTTHKSLRGPRG 
Sbjct: 235 ARLYDYARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGA 294

Query: 320 MIFFRK--------DPILGVDLESAINNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFRV 379
           MIFFRK           +  D E  IN AVFPGLQGGPHNHTI GLAV LK A++PE++ 
Sbjct: 295 MIFFRKGLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKA 354

Query: 380 YQNKVVSNCRALASRLVELGYKLVSGGSDNHLVLVDLRPLGIDGARVEKILDMASITLNK 439
           YQ++V+ NC   A  L+  GY LVSGG+DNHLVLV+L+  GIDG+RVEK+L++  I  NK
Sbjct: 355 YQDQVLRNCSKFAETLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKVLELVHIAANK 414

Query: 440 NSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQITLNAKQLAPGSKLQE 499
           N+VPGD SA+VPGGIR+G+PA+T+RGF E++F  VA++    V+I L  K  + G+KL++
Sbjct: 415 NTVPGDVSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIKAESQGTKLKD 474

Query: 500 FLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPG 526
           F+  +++ +  L   +S+LR  VE    QFP  G
Sbjct: 475 FVATMQSNE-KLQSEMSKLREMVEEYAKQFPTIG 507

[Close]

BLAST of MC10g0817 vs. TAIR 10
Match: AT5G26780.3 (serine hydroxymethyltransferase 2 )

HSP 1 Score: 559.7 bits (1441), Expect = 2.5e-159
Identity = 278/470 (59.15%), Postives = 343/470 (72.98%), Query Frame = 0


Query: 80  LSETDPEVRSIINKEKQRQFKSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGG 139
           L E DPEV  II  EK RQ+K  ELI SENFTS +VM+AVGS +TNKYSEG PG RYYGG
Sbjct: 55  LDEIDPEVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGG 114

Query: 140 NEHIDELETLCQQRALAAFHLDENKWGVNVQPLSGSPANFEVYTAVLSPHDRIMGLDLPH 199
           NE+ID  ETLCQ+RAL AF LD +KWGVNVQ LSGSPANF+VYTA+L PH+RIM LDLPH
Sbjct: 115 NEYIDMAETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLPH 174

Query: 200 GGHLSHGFMTPKRRVSGTSIYFESMPYRLDETTGIVDYDMLEKTANLFRPKLIIAGASAY 259
           GGHLSHG+ T  +++S  SI+FE+MPYRLDE TG +DYD LEK+A LFRPKLI+AGASAY
Sbjct: 175 GGHLSHGYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASAY 234

Query: 260 PRDFDYPRMRKIADAVGAFLMMDMAHISGLVAASVVADPFEYCDIVTTTTHKSLRGPRGG 319
            R +DY R+RK+ +   A ++ DMAHISGLVAA V+  PFEY D+VTTTTHKSLRGPRG 
Sbjct: 235 ARLYDYARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGA 294

Query: 320 MIFFRK--------DPILGVDLESAINNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFRV 379
           MIFFRK           +  D E  IN AVFPGLQGGPHNHTI GLAV LK A++PE++ 
Sbjct: 295 MIFFRKGLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKA 354

Query: 380 YQNKVVSNCRALA----------------SRLVELGYKLVSGGSDNHLVLVDLRPLGIDG 439
           YQ++V+ NC   A                  L+  GY LVSGG+DNHLVLV+L+  GIDG
Sbjct: 355 YQDQVLRNCSKFAELDIRPTVIISYGLSMQTLLAKGYDLVSGGTDNHLVLVNLKNKGIDG 414

Query: 440 ARVEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEKEFIAVADFIHEGVQ 499
           +RVEK+L++  I  NKN+VPGD SA+VPGGIR+G+PA+T+RGF E++F  VA++    V+
Sbjct: 415 SRVEKVLELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVK 474

Query: 500 ITLNAKQLAPGSKLQEFLKFVKTTDFPLVDRVSELRSRVEALTTQFPIPG 526
           I L  K  + G+KL++F+  +++ +  L   +S+LR  VE    QFP  G
Sbjct: 475 IALKIKAESQGTKLKDFVATMQSNE-KLQSEMSKLREMVEEYAKQFPTIG 523

The following BLAST results are available for this feature:

BLAST of MC10g0817 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94JQ3	3.0e-242	79.02	Serine hydroxymethyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
Q9SZJ5	3.7e-163	58.20	Serine hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702...	[more]
P34899	8.2e-163	58.56	Serine hydroxymethyltransferase, mitochondrial OS=Pisum sativum OX=3888 PE=1 SV=...	[more]
P34898	1.8e-162	60.52	Serine hydroxymethyltransferase, cytosolic OS=Neurospora crassa (strain ATCC 246...	[more]
P50433	5.3e-162	61.23	Serine hydroxymethyltransferase, mitochondrial OS=Solanum tuberosum OX=4113 PE=2...	[more]

BLAST of MC10g0817 vs. NCBI nr


Match Name	Identity	Description
XP_022136789.1	100.00	serine hydroxymethyltransferase 3, chloroplastic [Momordica charantia]	[more]
XP_011653260.1	92.80	serine hydroxymethyltransferase 3, chloroplastic [Cucumis sativus] >XP_031740593...	[more]
XP_022942709.1	92.80	serine hydroxymethyltransferase 3, chloroplastic-like [Cucurbita moschata] >XP_0...	[more]
XP_022980294.1	92.80	serine hydroxymethyltransferase 3, chloroplastic-like [Cucurbita maxima] >XP_022...	[more]
XP_008451853.1	92.61	PREDICTED: serine hydroxymethyltransferase 3, chloroplastic [Cucumis melo] >TYK1...	[more]

BLAST of MC10g0817 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1C5C3	100.00	Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111008402 P...	[more]
A0A0A0KYS6	92.80	Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_4G062380 PE=3 ...	[more]
A0A6J1FS47	92.80	Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447665 PE...	[more]
A0A6J1IVW3	92.80	Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111479708 PE=3...	[more]
A0A5D3D173	92.61	Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]

BLAST of MC10g0817 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G32520.1	2.2e-243	79.02	serine hydroxymethyltransferase 3	[more]
AT4G32520.2	2.2e-243	79.02	serine hydroxymethyltransferase 3	[more]
AT4G37930.1	2.6e-164	58.20	serine transhydroxymethyltransferase 1	[more]
AT5G26780.1	1.9e-162	61.23	serine hydroxymethyltransferase 2	[more]
AT5G26780.3	2.5e-159	59.15	serine hydroxymethyltransferase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015422	Pyridoxal phosphate-dependent transferase, small domain	GENE3D	3.90.1150.10	Aspartate Aminotransferase, domain 1	coord: 84..524 e-value: 9.0E-214 score: 711.8
IPR015421	Pyridoxal phosphate-dependent transferase, major domain	GENE3D	3.40.640.10		coord: 109..374 e-value: 9.0E-214 score: 711.8
IPR039429	Serine hydroxymethyltransferase-like domain	PFAM	PF00464	SHMT	coord: 80..470 e-value: 6.5E-194 score: 644.0
IPR001085	Serine hydroxymethyltransferase	PIRSF	PIRSF000412	SHMT	coord: 66..526 e-value: 2.4E-175 score: 580.3
IPR001085	Serine hydroxymethyltransferase	PANTHER	PTHR11680	SERINE HYDROXYMETHYLTRANSFERASE	coord: 50..526
IPR001085	Serine hydroxymethyltransferase	HAMAP	MF_00051	SHMT	coord: 79..525 score: 44.189941
IPR001085	Serine hydroxymethyltransferase	CDD	cd00378	SHMT	coord: 82..494 e-value: 0.0 score: 645.797
None	No IPR available	PANTHER	PTHR11680:SF34	SERINE HYDROXYMETHYLTRANSFERASE	coord: 50..526
IPR019798	Serine hydroxymethyltransferase, pyridoxal phosphate binding site	PROSITE	PS00096	SHMT	coord: 303..319
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 76..525

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g0817.1	MC10g0817.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046655	folic acid metabolic process
biological_process	GO:0019264	glycine biosynthetic process from serine
biological_process	GO:0006565	L-serine catabolic process
biological_process	GO:0032259	methylation
biological_process	GO:0035999	tetrahydrofolate interconversion
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004372	glycine hydroxymethyltransferase activity
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0070905	serine binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0003824	catalytic activity