MC10g0649 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g0649
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionATP synthase subunit alpha, chloroplastic
LocationMC10: 5435294 .. 5435530 (+)
RNA-Seq ExpressionMC10g0649
SyntenyMC10g0649
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAATGGCAGGTGAATTAGTAGAATTTGAAGAGGGTACTATAAGCATAGCTCTAAATTTAGAATCAAATAATGTTGGTGTTGTATTAATGGGTGATGGTTTGCTGATACAGGAGGGAAGTTCTGTAAAAGCAACAGGAAGAATTGCTCAGATACCAATAACCGAGGCTTATTTAGGTCATGTTATAAATGCTCTGGCTAAACCTATTGATGGCCGACGTGAAATTTCAGCTTCTGAA

mRNA sequence

GTAATGGCAGGTGAATTAGTAGAATTTGAAGAGGGTACTATAAGCATAGCTCTAAATTTAGAATCAAATAATGTTGGTGTTGTATTAATGGGTGATGGTTTGCTGATACAGGAGGGAAGTTCTGTAAAAGCAACAGGAAGAATTGCTCAGATACCAATAACCGAGGCTTATTTAGGTCATGTTATAAATGCTCTGGCTAAACCTATTGATGGCCGACGTGAAATTTCAGCTTCTGAA

Coding sequence (CDS)

GTAATGGCAGGTGAATTAGTAGAATTTGAAGAGGGTACTATAAGCATAGCTCTAAATTTAGAATCAAATAATGTTGGTGTTGTATTAATGGGTGATGGTTTGCTGATACAGGAGGGAAGTTCTGTAAAAGCAACAGGAAGAATTGCTCAGATACCAATAACCGAGGCTTATTTAGGTCATGTTATAAATGCTCTGGCTAAACCTATTGATGGCCGACGTGAAATTTCAGCTTCTGAA

Protein sequence

VMAGELVEFEEGTISIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPITEAYLGHVINALAKPIDGRREISASE
Homology
BLAST of MC10g0649 vs. ExPASy Swiss-Prot
Match: Q0G9N4 (ATP synthase subunit alpha, chloroplastic OS=Liriodendron tulipifera OX=3415 GN=atpA PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 2.0e-33
Identity = 74/79 (93.67%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   VMAGELVEFEEGTISIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPITEAYLGH 60
           VMAGELVEFEEGTI IALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIP++EAYLG 
Sbjct: 48  VMAGELVEFEEGTIGIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPVSEAYLGR 107

Query: 61  VINALAKPIDGRREISASE 80
           VINALAKPIDGR EISASE
Sbjct: 108 VINALAKPIDGRGEISASE 126

BLAST of MC10g0649 vs. ExPASy Swiss-Prot
Match: Q3C1H4 (ATP synthase subunit alpha, chloroplastic OS=Nicotiana sylvestris OX=4096 GN=atpA PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 2.0e-33
Identity = 74/79 (93.67%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   VMAGELVEFEEGTISIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPITEAYLGH 60
           VMAGELVEFEEGTI IALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIP++EAYLG 
Sbjct: 48  VMAGELVEFEEGTIGIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPVSEAYLGR 107

Query: 61  VINALAKPIDGRREISASE 80
           VINALAKPIDGR EISASE
Sbjct: 108 VINALAKPIDGRGEISASE 126

BLAST of MC10g0649 vs. ExPASy Swiss-Prot
Match: Q33C53 (ATP synthase subunit alpha, chloroplastic OS=Nicotiana tomentosiformis OX=4098 GN=atpA PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 2.0e-33
Identity = 74/79 (93.67%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   VMAGELVEFEEGTISIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPITEAYLGH 60
           VMAGELVEFEEGTI IALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIP++EAYLG 
Sbjct: 48  VMAGELVEFEEGTIGIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPVSEAYLGR 107

Query: 61  VINALAKPIDGRREISASE 80
           VINALAKPIDGR EISASE
Sbjct: 108 VINALAKPIDGRGEISASE 126

BLAST of MC10g0649 vs. ExPASy Swiss-Prot
Match: P00823 (ATP synthase subunit alpha, chloroplastic OS=Nicotiana tabacum OX=4097 GN=atpA PE=2 SV=2)

HSP 1 Score: 142.5 bits (358), Expect = 2.0e-33
Identity = 74/79 (93.67%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   VMAGELVEFEEGTISIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPITEAYLGH 60
           VMAGELVEFEEGTI IALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIP++EAYLG 
Sbjct: 48  VMAGELVEFEEGTIGIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPVSEAYLGR 107

Query: 61  VINALAKPIDGRREISASE 80
           VINALAKPIDGR EISASE
Sbjct: 108 VINALAKPIDGRGEISASE 126

BLAST of MC10g0649 vs. ExPASy Swiss-Prot
Match: Q8S8Y3 (ATP synthase subunit alpha, chloroplastic OS=Atropa belladonna OX=33113 GN=atpA PE=3 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 2.6e-33
Identity = 73/79 (92.41%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   VMAGELVEFEEGTISIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPITEAYLGH 60
           VMAGELVEFEEGTI IALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIP++EAYLG 
Sbjct: 48  VMAGELVEFEEGTIGIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPVSEAYLGR 107

Query: 61  VINALAKPIDGRREISASE 80
           V+NALAKPIDGR EISASE
Sbjct: 108 VVNALAKPIDGRGEISASE 126

BLAST of MC10g0649 vs. NCBI nr
Match: OMP12586.1 (hypothetical protein COLO4_02994 [Corchorus olitorius])

HSP 1 Score: 140 bits (353), Expect = 8.19e-41
Identity = 72/79 (91.14%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   VMAGELVEFEEGTISIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPITEAYLGH 60
           VMAGELVEFEEGTI IALNLESNNVGVVLMGDGL+IQEGSSVKATG+IAQIP++EAYLG 
Sbjct: 48  VMAGELVEFEEGTIGIALNLESNNVGVVLMGDGLMIQEGSSVKATGKIAQIPVSEAYLGR 107

Query: 61  VINALAKPIDGRREISASE 79
           VINALAKPIDGR EISASE
Sbjct: 108 VINALAKPIDGRGEISASE 126

BLAST of MC10g0649 vs. NCBI nr
Match: MBE2321281.1 (F0F1 ATP synthase subunit alpha [Solirubrobacter sp. CPCC 204708])

HSP 1 Score: 140 bits (354), Expect = 8.82e-41
Identity = 73/79 (92.41%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   VMAGELVEFEEGTISIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPITEAYLGH 60
           VMAGELVEFEEGTI IALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIP++EA+LG 
Sbjct: 48  VMAGELVEFEEGTIGIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPVSEAFLGR 107

Query: 61  VINALAKPIDGRREISASE 79
           VINALAKPIDGR EISASE
Sbjct: 108 VINALAKPIDGRGEISASE 126

BLAST of MC10g0649 vs. NCBI nr
Match: DAD19263.1 (TPA_asm: hypothetical protein HUJ06_020726 [Nelumbo nucifera])

HSP 1 Score: 140 bits (352), Expect = 8.84e-41
Identity = 72/78 (92.31%), Postives = 75/78 (96.15%), Query Frame = 0

Query: 2  MAGELVEFEEGTISIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPITEAYLGHV 61
          MAGELVEFEEGTI IALNLESNNVGVVLMGDGL+IQEGSSVKATGRIAQIP++EAYLG V
Sbjct: 1  MAGELVEFEEGTIGIALNLESNNVGVVLMGDGLMIQEGSSVKATGRIAQIPVSEAYLGRV 60

Query: 62 INALAKPIDGRREISASE 79
          INALAKPIDGR EISASE
Sbjct: 61 INALAKPIDGRGEISASE 78

BLAST of MC10g0649 vs. NCBI nr
Match: OMP12370.1 (hypothetical protein COLO4_03276 [Corchorus olitorius])

HSP 1 Score: 140 bits (353), Expect = 9.53e-41
Identity = 72/79 (91.14%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   VMAGELVEFEEGTISIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPITEAYLGH 60
           VMAGELVEFEEGTI IALNLESNNVGVVLMGDGL+IQEGSSVKATG+IAQIP++EAYLG 
Sbjct: 48  VMAGELVEFEEGTIGIALNLESNNVGVVLMGDGLMIQEGSSVKATGKIAQIPVSEAYLGR 107

Query: 61  VINALAKPIDGRREISASE 79
           VINALAKPIDGR EISASE
Sbjct: 108 VINALAKPIDGRGEISASE 126

BLAST of MC10g0649 vs. NCBI nr
Match: VVW84905.1 (unnamed protein product, partial [Nymphaea colorata])

HSP 1 Score: 139 bits (350), Expect = 1.63e-40
Identity = 72/79 (91.14%), Postives = 75/79 (94.94%), Query Frame = 0

Query: 1   VMAGELVEFEEGTISIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPITEAYLGH 60
           VMAGELVEFEEGTI IALNLESNNVGVVLMGDGL+IQEGSSVKATGRIAQIP++EAYLG 
Sbjct: 38  VMAGELVEFEEGTIGIALNLESNNVGVVLMGDGLMIQEGSSVKATGRIAQIPVSEAYLGR 97

Query: 61  VINALAKPIDGRREISASE 79
           VINALAKPIDGR EI ASE
Sbjct: 98  VINALAKPIDGRGEILASE 116

BLAST of MC10g0649 vs. ExPASy TrEMBL
Match: A0A1R3KZQ1 (ATP-synt_ab_N domain-containing protein OS=Corchorus olitorius OX=93759 GN=COLO4_02994 PE=3 SV=1)

HSP 1 Score: 140 bits (353), Expect = 3.97e-41
Identity = 72/79 (91.14%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   VMAGELVEFEEGTISIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPITEAYLGH 60
           VMAGELVEFEEGTI IALNLESNNVGVVLMGDGL+IQEGSSVKATG+IAQIP++EAYLG 
Sbjct: 48  VMAGELVEFEEGTIGIALNLESNNVGVVLMGDGLMIQEGSSVKATGKIAQIPVSEAYLGR 107

Query: 61  VINALAKPIDGRREISASE 79
           VINALAKPIDGR EISASE
Sbjct: 108 VINALAKPIDGRGEISASE 126

BLAST of MC10g0649 vs. ExPASy TrEMBL
Match: A0A1R3KZ81 (ATP-synt_ab_N domain-containing protein OS=Corchorus olitorius OX=93759 GN=COLO4_03276 PE=3 SV=1)

HSP 1 Score: 140 bits (353), Expect = 4.62e-41
Identity = 72/79 (91.14%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   VMAGELVEFEEGTISIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPITEAYLGH 60
           VMAGELVEFEEGTI IALNLESNNVGVVLMGDGL+IQEGSSVKATG+IAQIP++EAYLG 
Sbjct: 48  VMAGELVEFEEGTIGIALNLESNNVGVVLMGDGLMIQEGSSVKATGKIAQIPVSEAYLGR 107

Query: 61  VINALAKPIDGRREISASE 79
           VINALAKPIDGR EISASE
Sbjct: 108 VINALAKPIDGRGEISASE 126

BLAST of MC10g0649 vs. ExPASy TrEMBL
Match: A0A5K1HAK6 (ATP-synt_ab_N domain-containing protein (Fragment) OS=Nymphaea colorata OX=210225 GN=NYM_LOCUS29093 PE=3 SV=1)

HSP 1 Score: 139 bits (350), Expect = 7.88e-41
Identity = 72/79 (91.14%), Postives = 75/79 (94.94%), Query Frame = 0

Query: 1   VMAGELVEFEEGTISIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPITEAYLGH 60
           VMAGELVEFEEGTI IALNLESNNVGVVLMGDGL+IQEGSSVKATGRIAQIP++EAYLG 
Sbjct: 38  VMAGELVEFEEGTIGIALNLESNNVGVVLMGDGLMIQEGSSVKATGRIAQIPVSEAYLGR 97

Query: 61  VINALAKPIDGRREISASE 79
           VINALAKPIDGR EI ASE
Sbjct: 98  VINALAKPIDGRGEILASE 116

BLAST of MC10g0649 vs. ExPASy TrEMBL
Match: A0A1R3L0F2 (ATP-synt_ab_N domain-containing protein OS=Corchorus olitorius OX=93759 GN=COLO4_02716 PE=3 SV=1)

HSP 1 Score: 140 bits (353), Expect = 1.20e-40
Identity = 72/79 (91.14%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   VMAGELVEFEEGTISIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPITEAYLGH 60
           VMAGELVEFEEGTI IALNLESNNVGVVLMGDGL+IQEGSSVKATG+IAQIP++EAYLG 
Sbjct: 48  VMAGELVEFEEGTIGIALNLESNNVGVVLMGDGLMIQEGSSVKATGKIAQIPVSEAYLGR 107

Query: 61  VINALAKPIDGRREISASE 79
           VINALAKPIDGR EISASE
Sbjct: 108 VINALAKPIDGRGEISASE 126

BLAST of MC10g0649 vs. ExPASy TrEMBL
Match: A0A5A4MK16 (ATP synthase CF1 alpha subunit (Fragment) OS=Abelmoschus esculentus OX=455045 PE=2 SV=1)

HSP 1 Score: 140 bits (353), Expect = 1.28e-40
Identity = 72/79 (91.14%), Postives = 76/79 (96.20%), Query Frame = 0

Query: 1   VMAGELVEFEEGTISIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPITEAYLGH 60
           VMAGELVEFEEGTI IALNLESNNVGVVLMGDGL+IQEGSSVKATG+IAQIP++EAYLG 
Sbjct: 48  VMAGELVEFEEGTIGIALNLESNNVGVVLMGDGLMIQEGSSVKATGKIAQIPVSEAYLGR 107

Query: 61  VINALAKPIDGRREISASE 79
           VINALAKPIDGR EISASE
Sbjct: 108 VINALAKPIDGRGEISASE 126

BLAST of MC10g0649 vs. TAIR 10
Match: ATCG00120.1 (ATP synthase subunit alpha )

HSP 1 Score: 137.1 bits (344), Expect = 6.1e-33
Identity = 70/79 (88.61%), Postives = 75/79 (94.94%), Query Frame = 0

Query: 1   VMAGELVEFEEGTISIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPITEAYLGH 60
           VMAGELVEFEEGTI IALNLESNNVGVVLMGDGL+IQEGSSVKATG+IAQIP++EAYLG 
Sbjct: 48  VMAGELVEFEEGTIGIALNLESNNVGVVLMGDGLMIQEGSSVKATGKIAQIPVSEAYLGR 107

Query: 61  VINALAKPIDGRREISASE 80
           VINALA PIDGR +ISASE
Sbjct: 108 VINALANPIDGRGKISASE 126

BLAST of MC10g0649 vs. TAIR 10
Match: AT2G07698.1 (ATPase, F1 complex, alpha subunit protein )

HSP 1 Score: 73.6 bits (179), Expect = 8.2e-14
Identity = 36/79 (45.57%), Postives = 50/79 (63.29%), Query Frame = 0

Query: 1   VMAGELVEFEEGTISIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPITEAYLGH 60
           + AGE+V F  G   +ALNLE+ NVG+V+ G    I+EG  VK TG I  +P  +A LG 
Sbjct: 319 IQAGEMVLFANGVKGMALNLENENVGIVVFGGDTAIKEGDLVKRTGSIVDVPAGKAMLGR 378

Query: 61  VINALAKPIDGRREISASE 80
           V++A+  PIDG+  +S  E
Sbjct: 379 VVDAMGVPIDGKGALSDHE 397

BLAST of MC10g0649 vs. TAIR 10
Match: ATMG01190.1 (ATP synthase subunit 1 )

HSP 1 Score: 73.6 bits (179), Expect = 8.2e-14
Identity = 36/79 (45.57%), Postives = 50/79 (63.29%), Query Frame = 0

Query: 1   VMAGELVEFEEGTISIALNLESNNVGVVLMGDGLLIQEGSSVKATGRIAQIPITEAYLGH 60
           + AGE+V F  G   +ALNLE+ NVG+V+ G    I+EG  VK TG I  +P  +A LG 
Sbjct: 49  IQAGEMVLFANGVKGMALNLENENVGIVVFGGDTAIKEGDLVKRTGSIVDVPAGKAMLGR 108

Query: 61  VINALAKPIDGRREISASE 80
           V++A+  PIDG+  +S  E
Sbjct: 109 VVDAMGVPIDGKGALSDHE 127

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0G9N42.0e-3393.67ATP synthase subunit alpha, chloroplastic OS=Liriodendron tulipifera OX=3415 GN=... [more]
Q3C1H42.0e-3393.67ATP synthase subunit alpha, chloroplastic OS=Nicotiana sylvestris OX=4096 GN=atp... [more]
Q33C532.0e-3393.67ATP synthase subunit alpha, chloroplastic OS=Nicotiana tomentosiformis OX=4098 G... [more]
P008232.0e-3393.67ATP synthase subunit alpha, chloroplastic OS=Nicotiana tabacum OX=4097 GN=atpA P... [more]
Q8S8Y32.6e-3392.41ATP synthase subunit alpha, chloroplastic OS=Atropa belladonna OX=33113 GN=atpA ... [more]
Match NameE-valueIdentityDescription
OMP12586.18.19e-4191.14hypothetical protein COLO4_02994 [Corchorus olitorius][more]
MBE2321281.18.82e-4192.41F0F1 ATP synthase subunit alpha [Solirubrobacter sp. CPCC 204708][more]
DAD19263.18.84e-4192.31TPA_asm: hypothetical protein HUJ06_020726 [Nelumbo nucifera][more]
OMP12370.19.53e-4191.14hypothetical protein COLO4_03276 [Corchorus olitorius][more]
VVW84905.11.63e-4091.14unnamed protein product, partial [Nymphaea colorata][more]
Match NameE-valueIdentityDescription
A0A1R3KZQ13.97e-4191.14ATP-synt_ab_N domain-containing protein OS=Corchorus olitorius OX=93759 GN=COLO4... [more]
A0A1R3KZ814.62e-4191.14ATP-synt_ab_N domain-containing protein OS=Corchorus olitorius OX=93759 GN=COLO4... [more]
A0A5K1HAK67.88e-4191.14ATP-synt_ab_N domain-containing protein (Fragment) OS=Nymphaea colorata OX=21022... [more]
A0A1R3L0F21.20e-4091.14ATP-synt_ab_N domain-containing protein OS=Corchorus olitorius OX=93759 GN=COLO4... [more]
A0A5A4MK161.28e-4091.14ATP synthase CF1 alpha subunit (Fragment) OS=Abelmoschus esculentus OX=455045 PE... [more]
Match NameE-valueIdentityDescription
ATCG00120.16.1e-3388.61ATP synthase subunit alpha [more]
AT2G07698.18.2e-1445.57ATPase, F1 complex, alpha subunit protein [more]
ATMG01190.18.2e-1445.57ATP synthase subunit 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023366ATP synthase subunit alpha, N-terminal domain-like superfamilyGENE3D2.40.30.20coord: 1..42
e-value: 1.2E-16
score: 62.2
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 43..79
e-value: 3.0E-5
score: 25.3
IPR004100ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domainPFAMPF02874ATP-synt_ab_Ncoord: 1..46
e-value: 6.1E-9
score: 36.3
NoneNo IPR availablePANTHERPTHR48082:SF6ATP SYNTHASE SUBUNIT ALPHA, CHLOROPLASTICcoord: 1..79
NoneNo IPR availablePANTHERPTHR48082ATP SYNTHASE SUBUNIT ALPHA, MITOCHONDRIALcoord: 1..79
IPR036121ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain superfamilySUPERFAMILY50615N-terminal domain of alpha and beta subunits of F1 ATP synthasecoord: 1..48

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g0649.1MC10g0649.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046034 ATP metabolic process
biological_process GO:0015986 ATP synthesis coupled proton transport
biological_process GO:1902600 proton transmembrane transport
cellular_component GO:0009507 chloroplast
cellular_component GO:0031090 organelle membrane
cellular_component GO:0045261 proton-transporting ATP synthase complex, catalytic core F(1)
molecular_function GO:0043531 ADP binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0046933 proton-transporting ATP synthase activity, rotational mechanism