MC10g0608 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g0608
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionSec14p-like phosphatidylinositol transfer family protein
LocationMC10: 5017174 .. 5022234 (-)
RNA-Seq ExpressionMC10g0608
SyntenyMC10g0608
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCGATTTTGCTCAAGGAGCTTCGCTTCCGGTTTCTTAGCCCCTCTGCTTGCTTCAGGTGCTTTTTCTGTAAATTTTCCGATCTGGTTTTGGAATCTTGCGATTTCTTCCACTCTGCTAGTGTGTGTAGTGTACTGGATGACTTGCTTGTATTCCGTCTTTATTTTAGAGTAGATCTGATTATTCCGAGTTTTGCCTCGATGGTTGTTGGTTTTTATCGGTTTGACTCTGATTTAGCTTGTCGTGGATTGGATTATCTACTCTCTTTCTACCTACCTCCTTTTAATTTTTCCTGTTTGTTCGGTTGTTTTCTCGGTTCGTTTTGTGTTCTCTGTTGTGGTCAACTGCATCGGTTTCCTTGTTGGATTTTGGTAATTGAGTAAAACTCTTGCCCTACGACTCTAGTTTTATTTCCGATTGCAATTCAATCTCGTGGATTATGATCATGTTCAGATAGTTCAATTTTCATTTTGTTCGGTTTGCAACCAAAGTTTCTTAGATAAGGGGAAGATCATGGACATATAAAGCGAGGGACAAACATCTCAATTGGTATTGGATGTTTTAGGTGAAATCAAAAGCAAAACCATGAGAGCTTAATATCATACCATTATGGAAAGGTGGAGGGTCGATTGTTCCTAAAACATTTAGTATTAGTTCGATTTATCAACATACTGGTAATTAATTTCGCAGTCTTGCTATATTCTGTTGTTTGGGGTGCTATTCCCTCCCTCTATGGATTGGATTGGTCTGTTGTCTGTACCGATTAGGGAGAACACTTACCCGCCTCCAGAAATCGAGTTCATAAAAAAGTTAAACAAATTGTTGCTTTTTCACTTTGTGAATTTAAAATGGATAATGAAATATGTAAGGATACAAGGATCAGCAGGTATCGTATAGCTACTAAATGGATAGTTTGGTTTTTACACTCTCCAGTCATGGACCCATACCGACAATCTTTTCCAATTTATTGATGCAATTTAATTGTGGAAAACTCTTTTTAGTTTTAAAATGTATTAGGACTTCCCCTTATATAATATAGAACTGTTAACGTTTATTTGAGCTTATTTACGGGGAAAACGGATAAATATTTGAGTCATTGGCTATTGTTCAGTCGAGATTTTCTAATCCTCCCCAATTTTGGTTCTCAATCCTCATTAATAACTAAAACTATTTTTTATGCTTTAAGAATTCTGTAAACAGTGGCATTATGTGATTAAACCTTTTCTAATCAGTGTCTGTAATGGCATTCTTCATAAAGTTTAGCTACAGACTGTTGAGGATATCAATCCTCACTGGAATAGCCTGACAAGTTTAAAGAATCCTTTATTTGTAGGTTTTGTTGCCGTACATTGTGCTCCATTGAAGATTGACATGTTTCGTTTAAAGAAGCATTCTCATAATCACCAGGAAAATGATTCTATACCAGAAGATGGAAAGGTAATAATTCATTCAATTCTTATCAACAACAGTTTCCATGTTGTTAGCTTGAAAGGTTGGATATAAATCCTATTGTGATTCTTTTGTTCTCTATCTTACATGTCTGGAAACTTATCTCAATCTACTGAAGTCAAATTTTAGTCTGGGTGATATATTTTTCAGGGGAAAGAATCCTAAAATCTTCGGGTCAGGCAAATTAGTTTCTTAGCATTGGAATATTATTTCCCATCCCAAATTTAATTTTTCATATCCTCTTGACAGTTTACAATTGTAGAATTGATTATCATTTTTGAAGTCCAGCACCCAATTCAATTCAACTGGATTAGGGTCATGCCACAACTACCCAACCCTACAACATCTAAAATGAAAATTAATTGCATCTTGCTTAATAACAAATTCCACCTACCTGACCCCATCCAAAACTAATACTGGTCACGAATTATTCAATGCTATATGTGTTCTCATTCTCTGTCAACCTTCGCCGTTTCTCTTTTGGTGAGTTAATGCTAATCCAATAAGTTTTGTTATGAAGATTAAAAGATTTAAGGACTCATTTCAAACTTATAAACTCTAAGAAGAATTAGAATCACATGATTAGTTGAAGGTGAATTAACTTTATTTTCATTCTGGATCTTGGAGATGATGTGAGATTTATTTTCATAATTGAATTGTTAATTTTTTAGTTTATGATGTTTATGCTCATGTTTTTTTTTGGTGTTTGTCTCTGTTTGGTTTGTTGTTGTCCTTCTTTTTTGTCATATCTTTTTTATTATCTTGTGCCTTGATTTTATGATGTGAGATCTTTTTTTTTTTCAAATGTAAGGAAAAAAGAAAGAAAGAATTTTTTTTTTTTTTTTTTTTTGAGGGAGGGCATATCTGAATACTTTTCTTGCTCAGATCGATGAACTTAGAGCTGCTCTTGGGCCTCTGTCTAGTCGAAGTTTAAAGTATTGCACTGATGCATGCCTGAGGAGATATTTGATAGCTCGAAACTGGAATGTTGACAAGTCAAGGAAAATGCTCGAAGAGACGCTCAAGTGGAGGGCAGCTTACAAGCCCGAAGAAATCCGTTGGGTTAGTTAAATTATGTCATTTTCCCTGCCTCAAACATCTAGAATAACAATTTTCGCAATCATAATATCTCTTGTTTTGTTTTGTTTTTTTTCATTTTATTATCTTCCCTTTGCTTCCTGCTGATTGTGCCTTCATAATGTGAATTTAAAGGTGTTTGAAAGCCATGGTTTGTTTTTTTTTAATTTAAAAAAAGGGCTCATAAATTGTCGTGATGGCTATGGAGTTAGAGCTACATAAAGATCTAATTAATGGATAGTAAATTACCAATGATTATTTTCACCTTCAGTTCTGTTTTTATTTAATGTATTTCAAAGTGAACTTTTGTAGTTTTGCTAGAAAGATTACTAAGAATAAAAAAGATATTGTTATTTTTGTCATTATATATATGTGTATATATATATTTTGTAAAGATTACTCGTACATGCACGGCTGTATGCCATAAAATCAAGATTTTTGAAGAAGCATTTTTCTCATACAGCATGAAGTAGCATTTGAAGGAGAGACTGGGAAAGTATCACGAGCAAATTTTCACGATCGACATGGAAGATCTGTGCTCATAATGAGACCAGGAATGCAGGTATTTTATATTTGCTCCCCTCCCAAGTCCTAACCAATATAATTTAAAATATTCATTAATATTGAGAATTCAATATTTTAAAGCCAACATGCTCAACTTGTCAAAGCATTTGTCATATATTCCCGACCAAAAGATTCTAGTTCAAACTCCATCCCTACATGTTTTTTATCGGAAAAATCCATATTTTAAAATAATGTTAGATAAATCACCATATTGGGACAAGTTGGAGTACAGGTAAAAACACCTGGAATAAGGAAAATATCTGAACACTCTTAAAGATGTAGCACAGGAGTCACGTGAAAATCAAAGAGAAATGAACAAAAGTGGTCCATTTACAATTTCTAAGTGCTTTTTTGGCTTTTGGGTTTTGGCATTGAGTATTTTTCTGACCATTTCCAGTTCTTCTGAAATGTTTCAGAACACAACTCCATCAGAAACGAGTGTGCGGCATCTTGTGTATCTTTTGGAAAATGCAATTCTAAACCTTGGGGAGGGTCAAGAACAAATGTGTTGGTTGATAGACTTCACCGGATTTACAATGAAAACTAATGTCTCTGTCAAAATAGCCGCAGATGTCATCAATGTTTTGCAGAACCATTACCCCGAGAGACTCGCCGTTTCACTCCTCTACAATCCACCCAGGTTTTTTCAAGCATTCTGGAAGGTACTCTCTCATCCTAACCACTTGTAACTCTAAAAGATTATATAATTTTTGTCTAGGCTAAGTATAAGTCCCTAATGTTTAGGTGTTTTTCTTTTAGATGTAGTCCTTATTGTTTTATAATATTTTATATCATTTTTCAATTTAATCCCTCACTCTTAAAGTTGGCGGATAAGTTTATATAGAATAATACTTAGTGAATTGGTAAAAATCTAGAGGAGGAAAAATGTCATCTAAGAGAGAGTTACTAATTTCTTCACCAAATTTCTTAGGAAATTGGTAGAAGTTTTGAGTTCTTTTTTCTTGATGAACTTTTTTCTCTTCTAAATTTTCATCAATTTACTAAGTATTATGTCATGTAAGCTTGTCACCAGCCAATTTTAACATTCATATATGATCGGAGACTAAATTGAGAAATATTGAAACACAAGGGACTAAATTGAGACTTTTTAAACGATAGAGATTAAATTGAAAAATCGTCCGGATTGAAATATCTTATTGACCTTTTTGTTTATTAATGCAAATAATTTTCTTTCAAATAAGTACTTCTATAGGCTTCATATGCAGCATCATTTAACTTTATTAGAAATCAAATTTGTCCTGAACATTAAAAAGTTTCATAATCATGATTAAGTTAGATAGATGGTAAAATTTTACGTTTTAATCCTTTTTATATATAATATTTCGATTATAAAAATTTAAGGAGCAAATTAGAGAGAACTAGAAAGGCAATTGCCTCACTCCGTCGTGCATTTGTGTGTATTTTGTTCGATGAACTGAACTAGGTGATTCTTCCAATTTTTGTAGGCCATAAAGTACTTCTTAGACCCAAAGACATTTCAAAAGGTAAAGTTTGTTAATCCCAAAGACAAAGGAAGCATTGAGCTGATGAAATCATACTTTGACATGGAAAATCTTCCTAGTGTCTTTGGGGGTAAAGCCACTCTCGAATATCACCACCAGGAGTTCTCCCAGATGATGGCCCAAGACGAGCTCAGAACTGCCCAATTTTGGGGCTTCGATAAGGCCCACCACATTGTCCATGGGCCTTCTGGCCCAGAAGTCCCACCAGAGCCCATTACTACTTCAGCTAACTAACTGGTATGCCTCATGCCCTTCAACAGCTTTTTCGAATTCAAATAATCAACTTTTCCTTCAACTACTCATCCATCACTAATACTTGCAGCTGAGTGCTAACTAACTATGAGAGTGAATGGTTTATGTTAACTTATGAGGTCAACAGAGCCCAATGATTTATTAAATATATTTGAAGACACATCTTCTTGTTCCTTCTTTAGAAGAATATCTTTAG

mRNA sequence

CTCCGATTTTGCTCAAGGAGCTTCGCTTCCGGTTTCTTAGCCCCTCTGCTTGCTTCAGGTTTTGTTGCCGTACATTGTGCTCCATTGAAGATTGACATGTTTCGTTTAAAGAAGCATTCTCATAATCACCAGGAAAATGATTCTATACCAGAAGATGGAAAGATCGATGAACTTAGAGCTGCTCTTGGGCCTCTGTCTAGTCGAAGTTTAAAGTATTGCACTGATGCATGCCTGAGGAGATATTTGATAGCTCGAAACTGGAATGTTGACAAGTCAAGGAAAATGCTCGAAGAGACGCTCAAGTGGAGGGCAGCTTACAAGCCCGAAGAAATCCGTTGGCATGAAGTAGCATTTGAAGGAGAGACTGGGAAAGTATCACGAGCAAATTTTCACGATCGACATGGAAGATCTGTGCTCATAATGAGACCAGGAATGCAGAACACAACTCCATCAGAAACGAGTGTGCGGCATCTTGTGTATCTTTTGGAAAATGCAATTCTAAACCTTGGGGAGGGTCAAGAACAAATGTGTTGGTTGATAGACTTCACCGGATTTACAATGAAAACTAATGTCTCTGTCAAAATAGCCGCAGATGTCATCAATGTTTTGCAGAACCATTACCCCGAGAGACTCGCCGTTTCACTCCTCTACAATCCACCCAGGTTTTTTCAAGCATTCTGGAAGGCCATAAAGTACTTCTTAGACCCAAAGACATTTCAAAAGGTAAAGTTTGTTAATCCCAAAGACAAAGGAAGCATTGAGCTGATGAAATCATACTTTGACATGGAAAATCTTCCTAGTGTCTTTGGGGGTAAAGCCACTCTCGAATATCACCACCAGGAGTTCTCCCAGATGATGGCCCAAGACGAGCTCAGAACTGCCCAATTTTGGGGCTTCGATAAGGCCCACCACATTGTCCATGGGCCTTCTGGCCCAGAAGTCCCACCAGAGCCCATTACTACTTCAGCTAACTAACTGGTATGCCTCATGCCCTTCAACAGCTTTTTCGAATTCAAATAATCAACTTTTCCTTCAACTACTCATCCATCACTAATACTTGCAGCTGAGTGCTAACTAACTATGAGAGTGAATGGTTTATGTTAACTTATGAGGTCAACAGAGCCCAATGATTTATTAAATATATTTGAAGACACATCTTCTTGTTCCTTCTTTAGAAGAATATCTTTAG

Coding sequence (CDS)

CTCCGATTTTGCTCAAGGAGCTTCGCTTCCGGTTTCTTAGCCCCTCTGCTTGCTTCAGGTTTTGTTGCCGTACATTGTGCTCCATTGAAGATTGACATGTTTCGTTTAAAGAAGCATTCTCATAATCACCAGGAAAATGATTCTATACCAGAAGATGGAAAGATCGATGAACTTAGAGCTGCTCTTGGGCCTCTGTCTAGTCGAAGTTTAAAGTATTGCACTGATGCATGCCTGAGGAGATATTTGATAGCTCGAAACTGGAATGTTGACAAGTCAAGGAAAATGCTCGAAGAGACGCTCAAGTGGAGGGCAGCTTACAAGCCCGAAGAAATCCGTTGGCATGAAGTAGCATTTGAAGGAGAGACTGGGAAAGTATCACGAGCAAATTTTCACGATCGACATGGAAGATCTGTGCTCATAATGAGACCAGGAATGCAGAACACAACTCCATCAGAAACGAGTGTGCGGCATCTTGTGTATCTTTTGGAAAATGCAATTCTAAACCTTGGGGAGGGTCAAGAACAAATGTGTTGGTTGATAGACTTCACCGGATTTACAATGAAAACTAATGTCTCTGTCAAAATAGCCGCAGATGTCATCAATGTTTTGCAGAACCATTACCCCGAGAGACTCGCCGTTTCACTCCTCTACAATCCACCCAGGTTTTTTCAAGCATTCTGGAAGGCCATAAAGTACTTCTTAGACCCAAAGACATTTCAAAAGGTAAAGTTTGTTAATCCCAAAGACAAAGGAAGCATTGAGCTGATGAAATCATACTTTGACATGGAAAATCTTCCTAGTGTCTTTGGGGGTAAAGCCACTCTCGAATATCACCACCAGGAGTTCTCCCAGATGATGGCCCAAGACGAGCTCAGAACTGCCCAATTTTGGGGCTTCGATAAGGCCCACCACATTGTCCATGGGCCTTCTGGCCCAGAAGTCCCACCAGAGCCCATTACTACTTCAGCTAACTAA

Protein sequence

LRFCSRSFASGFLAPLLASGFVAVHCAPLKIDMFRLKKHSHNHQENDSIPEDGKIDELRAALGPLSSRSLKYCTDACLRRYLIARNWNVDKSRKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFHDRHGRSVLIMRPGMQNTTPSETSVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADVINVLQNHYPERLAVSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSIELMKSYFDMENLPSVFGGKATLEYHHQEFSQMMAQDELRTAQFWGFDKAHHIVHGPSGPEVPPEPITTSAN
Homology
BLAST of MC10g0608 vs. ExPASy Swiss-Prot
Match: Q9UU99 (CRAL-TRIO domain-containing protein C23B6.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC23B6.04c PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.1e-29
Identity = 77/211 (36.49%), Postives = 112/211 (53.08%), Query Frame = 0

Query: 72  YCTDACLRRYLIARNWNVDKSRKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFH 131
           + T  C+ RYL A  W+V  ++K + +TL WR  +    +   E+  E  TGK     + 
Sbjct: 632 WLTRECILRYLRATKWHVSNAKKRIVDTLVWRRHFGVNNMDPDEIQEENATGKQVLLGY- 691

Query: 132 DRHGRSVLIMRPGMQNTTPSETSVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNV 191
           D+ GR  L + P  QNT  S   +RHLV+ LE AI  +  G E +  LI+F   + ++N 
Sbjct: 692 DKDGRPCLYLYPARQNTKTSPLQIRHLVFSLECAIDLMPPGVETLALLINFKSSSNRSNP 751

Query: 192 SVKIAADVINVLQNHYPERLAVSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVNPKDKG 251
           SV    +V+N+LQ HY ERL  +L+ N P     F+K I  F+DP T +K+KF  P D+ 
Sbjct: 752 SVGQGKEVLNILQTHYCERLGRALVINIPWAVWGFFKLISPFIDPITREKLKFNEPLDR- 811

Query: 252 SIELMKSYFDMENLPSVFGGKATLEYHHQEF 283
                  Y   + L S FGG    EYHH+++
Sbjct: 812 -------YVPKDQLDSNFGGSLHFEYHHEKY 833

BLAST of MC10g0608 vs. ExPASy Swiss-Prot
Match: Q55CU8 (Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=pitC PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.5e-29
Identity = 78/221 (35.29%), Postives = 117/221 (52.94%), Query Frame = 0

Query: 70  LKYCTDACLRRYLIARNWNVDKSRKMLEETLKWRAAYKPEEIR-WHEVAFEGETGKVSRA 129
           + +CTD C  RYL ARN+ V KS KML +TL+WR  ++P++I+   ++   G  G V   
Sbjct: 68  IAFCTDMCFLRYLRARNYIVSKSEKMLRDTLEWRKKFRPQDIQLGGDIREIGSAGCV-YV 127

Query: 130 NFHDRHGRSVL--IMRPGMQNTTPSETSVRHLVYLLENAILNLGE--GQEQMCWLIDFTG 189
           N  D+ GR ++  + R       PSE   ++LVY LE     + E  G EQ C+++D+  
Sbjct: 128 NKRDKKGRPIIFAVPRNDTLKNVPSELKFKNLVYWLEQGFSRMDEPKGIEQFCFIVDYKD 187

Query: 190 FTMKTNVSVKIAADVINVLQNHYPERLAVSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKF 249
           F    N+ +K   + ++ L +H PER+  SL  +PP  F   WK I  FL+  T  KV+F
Sbjct: 188 FG-SGNMDMKTNLEAMHFLLDHCPERMGQSLFLDPPALFWFAWKIISPFLNEVTLSKVRF 247

Query: 250 VNPK---DKGSIELMKSYFDMENLPSVFGGKATLEYHHQEF 283
           +N K    K +   +  Y D+ENL    GG     Y+  E+
Sbjct: 248 INSKKVDGKRTFAELLEYVDIENLEQNLGGNLDYNYNIDEY 286

BLAST of MC10g0608 vs. ExPASy Swiss-Prot
Match: P53844 (Phosphatidylinositol transfer protein PDR17 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PDR17 PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 2.7e-23
Identity = 83/257 (32.30%), Postives = 121/257 (47.08%), Query Frame = 0

Query: 64  PLSSRSLKYCTDACLRRYLIARNWNVDKSRKMLEETLKWR-------AAYKPEEIRWHEV 123
           PLS     + +  C  RYL A  WN   + K L +TL WR            + +   +V
Sbjct: 81  PLSDWEKFWLSRECFLRYLRANKWNTANAIKGLTKTLVWRREIGLTHGKEDKDPLTADKV 140

Query: 124 AFEGETGKVSRANFHDRHGRSVLIMRPGMQNTTPSETSVRHLVYLLENAILNLGEGQEQM 183
           A E ETGK     F D   R +  M+ G QNT  S   V+ LVY++E A     +G E++
Sbjct: 141 AVENETGKQVILGF-DNAKRPLYYMKNGRQNTESSFRQVQELVYMMETATTVAPQGVEKI 200

Query: 184 CWLIDFTGFTMKTNVS-----VKIAADVINVLQNHYPERLAVSLLYNPPRFFQAFWKAIK 243
             L+DF  +     ++     + IA   +NV+Q+HYPERLA  +L N P F  AF K + 
Sbjct: 201 TVLVDFKSYKEPGIITDKAPPISIARMCLNVMQDHYPERLAKCVLINIPWFAWAFLKMMY 260

Query: 244 YFLDPKTFQKVKFVNPKDKGSIELMKSYFDMENLPSVFGGKATLEYHHQEFSQMMAQ--D 303
            FLDP T  K  F  P         +++ +   L +++ G    +Y H+ +   M +  D
Sbjct: 261 PFLDPATKAKAIFDEP--------FENHIEPSQLDALYNGLLDFKYKHEVYWPDMVKKVD 320

Query: 304 ELRTAQFWGFDKAHHIV 307
           +LR  +F  F K   IV
Sbjct: 321 DLRLKRFDRFLKFGGIV 328

BLAST of MC10g0608 vs. ExPASy Swiss-Prot
Match: P53860 (Phosphatidylinositol transfer protein PDR16 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PDR16 PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 6.0e-23
Identity = 74/236 (31.36%), Postives = 108/236 (45.76%), Query Frame = 0

Query: 62  LGPLSSRSLKYCTDACLRRYLIARNWNVDKSRKMLEETLKWRAAY--------KPEEIRW 121
           L PL      + T  C  RYL A  W +      +  TL WR  +          ++I  
Sbjct: 74  LKPLEEEEKAWLTRECFLRYLRATKWVLKDCIDRITMTLAWRREFGISHLGEEHGDKITA 133

Query: 122 HEVAFEGETGKVSRANFHDRHGRSVLIMRPGMQNTTPSETSVRHLVYLLENAILNLGEGQ 181
             VA E E+GK     + +   R +L ++PG QNT  S   V+HLV++LE  I  +  GQ
Sbjct: 134 DLVAVENESGKQVILGY-ENDARPILYLKPGRQNTKTSHRQVQHLVFMLERVIDFMPAGQ 193

Query: 182 EQMCWLIDFTGFTMKTNV-------SVKIAADVINVLQNHYPERLAVSLLYNPPRFFQAF 241
           + +  LIDF  +     V        + +  +V+++LQ HYPERL  +LL N P     F
Sbjct: 194 DSLALLIDFKDYPDVPKVPGNSKIPPIGVGKEVLHILQTHYPERLGKALLTNIPWLAWTF 253

Query: 242 WKAIKYFLDPKTFQKVKFVNPKDKGSIELMKSYFDMENLPSVFGGKATLEYHHQEF 283
            K I  F+DP T +K+ F  P  K        Y     L S++GG    +Y+H  +
Sbjct: 254 LKLIHPFIDPLTREKLVFDEPFVK--------YVPKNELDSLYGGDLKFKYNHDVY 300

BLAST of MC10g0608 vs. ExPASy Swiss-Prot
Match: O43304 (SEC14-like protein 5 OS=Homo sapiens OX=9606 GN=SEC14L5 PE=1 SV=3)

HSP 1 Score: 76.3 bits (186), Expect = 7.3e-13
Identity = 60/251 (23.90%), Postives = 108/251 (43.03%), Query Frame = 0

Query: 75  DACLRRYLIARNWNVDKSRKMLEETLKWRAAYKPEEI--RWHEVAFEGETGKVSRANFHD 134
           D  + R+L A ++++DK+R+ML ++L WR  ++ + +   W   A   E       ++ D
Sbjct: 264 DEHILRFLRAHDFHLDKAREMLRQSLSWRKQHQVDLLLQTWQPPALL-EEFYAGGWHYQD 323

Query: 135 RHGRSVLIMRPGMQNT------TPSETSVRHLVYL-------LENAILNLGEGQEQMCWL 194
             GR + I+R G  +T         E  +RH++ +        E +   LG        L
Sbjct: 324 IDGRPLYILRLGQMDTKGLMKAVGEEALLRHVLSVNEEGQKRCEGSTRQLGRPISSWTCL 383

Query: 195 IDFTGFTMK--TNVSVKIAADVINVLQNHYPERLAVSLLYNPPRFFQAFWKAIKYFLDPK 254
           +D  G  M+      VK    +I V++++YPE L   L+   PR F   W  I  F++  
Sbjct: 384 LDLEGLNMRHLWRPGVKALLRMIEVVEDNYPETLGRLLIVRAPRVFPVLWTLISPFINEN 443

Query: 255 TFQKVKFVNPKDKGSIELMKSYFDMENLPSVFGGKATLEYHH----QEFSQMMAQDELRT 305
           T +K    +  +      +  Y D E +P   GG++           +   M  +++  T
Sbjct: 444 TRRKFLIYSGSNYQGPGGLVDYLDREVIPDFLGGESVCNVPEGGLVPKSLYMTEEEQEHT 503

BLAST of MC10g0608 vs. NCBI nr
Match: XP_022136351.1 (random slug protein 5-like [Momordica charantia])

HSP 1 Score: 604 bits (1557), Expect = 1.27e-217
Identity = 292/292 (100.00%), Postives = 292/292 (100.00%), Query Frame = 0

Query: 33  MFRLKKHSHNHQENDSIPEDGKIDELRAALGPLSSRSLKYCTDACLRRYLIARNWNVDKS 92
           MFRLKKHSHNHQENDSIPEDGKIDELRAALGPLSSRSLKYCTDACLRRYLIARNWNVDKS
Sbjct: 1   MFRLKKHSHNHQENDSIPEDGKIDELRAALGPLSSRSLKYCTDACLRRYLIARNWNVDKS 60

Query: 93  RKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFHDRHGRSVLIMRPGMQNTTPSE 152
           RKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFHDRHGRSVLIMRPGMQNTTPSE
Sbjct: 61  RKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFHDRHGRSVLIMRPGMQNTTPSE 120

Query: 153 TSVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADVINVLQNHYPERLA 212
           TSVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADVINVLQNHYPERLA
Sbjct: 121 TSVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADVINVLQNHYPERLA 180

Query: 213 VSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSIELMKSYFDMENLPSVFGGK 272
           VSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSIELMKSYFDMENLPSVFGGK
Sbjct: 181 VSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSIELMKSYFDMENLPSVFGGK 240

Query: 273 ATLEYHHQEFSQMMAQDELRTAQFWGFDKAHHIVHGPSGPEVPPEPITTSAN 324
           ATLEYHHQEFSQMMAQDELRTAQFWGFDKAHHIVHGPSGPEVPPEPITTSAN
Sbjct: 241 ATLEYHHQEFSQMMAQDELRTAQFWGFDKAHHIVHGPSGPEVPPEPITTSAN 292

BLAST of MC10g0608 vs. NCBI nr
Match: KAA0044746.1 (random slug protein 5 [Cucumis melo var. makuwa] >TYK16716.1 random slug protein 5 [Cucumis melo var. makuwa])

HSP 1 Score: 540 bits (1390), Expect = 1.12e-191
Identity = 265/307 (86.32%), Postives = 284/307 (92.51%), Query Frame = 0

Query: 21  FVAVHCAPLKIDMFRLKKHSHNH---QENDSIPEDGKIDELRAALGPLSSRSLKYCTDAC 80
           ++A HCAPL IDMFR KKHS +H   Q+NDS  E+GKI+ELRAALGPLSSRS KYCTDAC
Sbjct: 16  YLAAHCAPLLIDMFRSKKHSQSHNNGQDNDSTQEEGKINELRAALGPLSSRSSKYCTDAC 75

Query: 81  LRRYLIARNWNVDKSRKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFHDRHGRS 140
           LRRYLIARNWNV+KSRKMLEETLKWRAAYKPEEIRW EVAFEGETGKVSRANF+DRHGRS
Sbjct: 76  LRRYLIARNWNVEKSRKMLEETLKWRAAYKPEEIRWDEVAFEGETGKVSRANFYDRHGRS 135

Query: 141 VLIMRPGMQNTTPSETSVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAA 200
           VLIMRPGMQNTTPSE SVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAA
Sbjct: 136 VLIMRPGMQNTTPSEASVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAA 195

Query: 201 DVINVLQNHYPERLAVSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSIELMK 260
           D+INVLQNHYPERLA + LYNPP+FFQAFWKAIKYFLDPKT QKVKFVNPKDKGS+ELMK
Sbjct: 196 DIINVLQNHYPERLAFAFLYNPPKFFQAFWKAIKYFLDPKTSQKVKFVNPKDKGSVELMK 255

Query: 261 SYFDMENLPSVFGGKATLEYHHQEFSQMMAQDELRTAQFWGFD-KAHHIVHG-PSGPEVP 320
           S+FDMENLPSVFGGKATLEY HQ+FSQMM QDEL+ AQFWGFD KAHH V+G  SGPEV 
Sbjct: 256 SHFDMENLPSVFGGKATLEYDHQQFSQMMGQDELKAAQFWGFDEKAHHSVNGHSSGPEVG 315

Query: 321 PEPITTS 322
           PEP+T++
Sbjct: 316 PEPVTST 322

BLAST of MC10g0608 vs. NCBI nr
Match: XP_038893006.1 (phosphatidylinositol transfer protein 3-like [Benincasa hispida] >XP_038893012.1 phosphatidylinositol transfer protein 3-like [Benincasa hispida])

HSP 1 Score: 532 bits (1371), Expect = 3.02e-189
Identity = 257/293 (87.71%), Postives = 277/293 (94.54%), Query Frame = 0

Query: 33  MFRLKKHS--HNHQENDSIPEDGKIDELRAALGPLSSRSLKYCTDACLRRYLIARNWNVD 92
           MFRLKKHS  HN Q+ DS  EDGKI+ELRAALGPLSSR+LKYCTDACLRRYLIARNWNV+
Sbjct: 1   MFRLKKHSQSHNRQDIDSTQEDGKINELRAALGPLSSRNLKYCTDACLRRYLIARNWNVE 60

Query: 93  KSRKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFHDRHGRSVLIMRPGMQNTTP 152
           KSRKMLEETLKWRAA+KPEEIRWHEVAFEGETGKVSRANF+DRHGRSVLIMRPGMQNTTP
Sbjct: 61  KSRKMLEETLKWRAAFKPEEIRWHEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTP 120

Query: 153 SETSVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADVINVLQNHYPER 212
           SE SVRHLVYLLENAI+NLGEGQEQMCWLIDF+GFTMKTN+SVKIA D+INVLQNHYPER
Sbjct: 121 SEASVRHLVYLLENAIMNLGEGQEQMCWLIDFSGFTMKTNISVKIAGDIINVLQNHYPER 180

Query: 213 LAVSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSIELMKSYFDMENLPSVFG 272
           LA + LYNPP+FFQAFWKAIKYFLDPKTFQKVKFVNPKDKGS+ELMKS+FDMENLPSVFG
Sbjct: 181 LAFAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFG 240

Query: 273 GKATLEYHHQEFSQMMAQDELRTAQFWGFDKAHHIVHGPS-GPEVPPEPITTS 322
           GKATLEY HQ+FSQMMAQDEL+ AQFWG D+ HHIV+GPS GPEV PEPIT++
Sbjct: 241 GKATLEYDHQQFSQMMAQDELKAAQFWGLDENHHIVNGPSSGPEVAPEPITST 293

BLAST of MC10g0608 vs. NCBI nr
Match: KAG6577254.1 (hypothetical protein SDJN03_24828, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 541 bits (1395), Expect = 1.46e-187
Identity = 270/318 (84.91%), Postives = 285/318 (89.62%), Query Frame = 0

Query: 9   ASGFLAPLLASGFVAVHCAPLKIDMFRLKKHS--HNHQENDSIPEDGKIDELRAALGPLS 68
           ASGFLA  L   F+A HC+PL IDMFR KKHS  HNH  NDS  EDGKI+ELRAALGPLS
Sbjct: 327 ASGFLALYLLQVFLAAHCSPLLIDMFRSKKHSQSHNHHNNDSTQEDGKINELRAALGPLS 386

Query: 69  SRSLKYCTDACLRRYLIARNWNVDKSRKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVS 128
           SRS KYCTDACLRRYLIARNWNV+K+RKMLEETLKWRAAYKPEEI WHEVAFEGETGKVS
Sbjct: 387 SRSSKYCTDACLRRYLIARNWNVEKARKMLEETLKWRAAYKPEEIHWHEVAFEGETGKVS 446

Query: 129 RANFHDRHGRSVLIMRPGMQNTTPSETSVRHLVYLLENAILNLGEGQEQMCWLIDFTGFT 188
           RA+F DR GRSVLIMRPGMQNTTPSE SVRHLVYLLENAILNL EGQEQMCWLIDFTGFT
Sbjct: 447 RADFCDRQGRSVLIMRPGMQNTTPSEVSVRHLVYLLENAILNLVEGQEQMCWLIDFTGFT 506

Query: 189 MKTNVSVKIAADVINVLQNHYPERLAVSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVN 248
           MKTNVSVKIA+DVIN+LQNHYPERLAV+ LYNPP+ FQAFWKAIKYFLDPKT QKVKFVN
Sbjct: 507 MKTNVSVKIASDVINILQNHYPERLAVAFLYNPPKIFQAFWKAIKYFLDPKTSQKVKFVN 566

Query: 249 PKDKGSIELMKSYFDMENLPSVFGGKATLEYHHQEFSQMMAQDELRTAQFWGFD-KAHHI 308
           PKDKGS+ELMKSYFDMENLPSVFGGKATLEY HQ+FSQMMAQDEL+ A  WG D KAHHI
Sbjct: 567 PKDKGSVELMKSYFDMENLPSVFGGKATLEYDHQQFSQMMAQDELKAALLWGLDEKAHHI 626

Query: 309 VHGPS-GPEVPPEPITTS 322
            +GPS GPEV PEPIT +
Sbjct: 627 TNGPSSGPEVAPEPITAT 644

BLAST of MC10g0608 vs. NCBI nr
Match: XP_023553217.1 (phosphatidylinositol transfer protein 3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023553218.1 phosphatidylinositol transfer protein 3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023553219.1 phosphatidylinositol transfer protein 3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023553220.1 phosphatidylinositol transfer protein 3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023553221.1 phosphatidylinositol transfer protein 3-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 522 bits (1344), Expect = 4.07e-185
Identity = 258/294 (87.76%), Postives = 272/294 (92.52%), Query Frame = 0

Query: 33  MFRLKKHS--HNHQENDSIPEDGKIDELRAALGPLSSRSLKYCTDACLRRYLIARNWNVD 92
           MFR KKHS  HNH++NDS  EDGKI+ELRAALGPLSSRS KYCTDACLRRYLIARNWNV+
Sbjct: 1   MFRSKKHSQSHNHRDNDSTQEDGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVE 60

Query: 93  KSRKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFHDRHGRSVLIMRPGMQNTTP 152
           K+RKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANF DR GRSVLIMRPGMQNTTP
Sbjct: 61  KARKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFCDRQGRSVLIMRPGMQNTTP 120

Query: 153 SETSVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADVINVLQNHYPER 212
           SE SVRHLVYLLENAILNL EGQEQMCWLIDFTGFTMKTNVSVKIA+DVIN+LQNHYPER
Sbjct: 121 SEVSVRHLVYLLENAILNLVEGQEQMCWLIDFTGFTMKTNVSVKIASDVINILQNHYPER 180

Query: 213 LAVSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSIELMKSYFDMENLPSVFG 272
           LAV+ LYNPP+ FQAFWKAIKYFLDPKT QKVKFVNPKDKGS+ELMKSYFDMENLPSVFG
Sbjct: 181 LAVAFLYNPPKIFQAFWKAIKYFLDPKTSQKVKFVNPKDKGSVELMKSYFDMENLPSVFG 240

Query: 273 GKATLEYHHQEFSQMMAQDELRTAQFWGFD-KAHHIVHGPS-GPEVPPEPITTS 322
           GKATLEY HQ+FSQMMAQDEL+ AQ WG D KAHHI +GPS GPEV PEPIT +
Sbjct: 241 GKATLEYDHQQFSQMMAQDELKAAQVWGLDEKAHHITNGPSSGPEVAPEPITAT 294

BLAST of MC10g0608 vs. ExPASy TrEMBL
Match: A0A6J1C3A4 (random slug protein 5-like OS=Momordica charantia OX=3673 GN=LOC111008083 PE=4 SV=1)

HSP 1 Score: 604 bits (1557), Expect = 6.13e-218
Identity = 292/292 (100.00%), Postives = 292/292 (100.00%), Query Frame = 0

Query: 33  MFRLKKHSHNHQENDSIPEDGKIDELRAALGPLSSRSLKYCTDACLRRYLIARNWNVDKS 92
           MFRLKKHSHNHQENDSIPEDGKIDELRAALGPLSSRSLKYCTDACLRRYLIARNWNVDKS
Sbjct: 1   MFRLKKHSHNHQENDSIPEDGKIDELRAALGPLSSRSLKYCTDACLRRYLIARNWNVDKS 60

Query: 93  RKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFHDRHGRSVLIMRPGMQNTTPSE 152
           RKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFHDRHGRSVLIMRPGMQNTTPSE
Sbjct: 61  RKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFHDRHGRSVLIMRPGMQNTTPSE 120

Query: 153 TSVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADVINVLQNHYPERLA 212
           TSVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADVINVLQNHYPERLA
Sbjct: 121 TSVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADVINVLQNHYPERLA 180

Query: 213 VSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSIELMKSYFDMENLPSVFGGK 272
           VSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSIELMKSYFDMENLPSVFGGK
Sbjct: 181 VSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSIELMKSYFDMENLPSVFGGK 240

Query: 273 ATLEYHHQEFSQMMAQDELRTAQFWGFDKAHHIVHGPSGPEVPPEPITTSAN 324
           ATLEYHHQEFSQMMAQDELRTAQFWGFDKAHHIVHGPSGPEVPPEPITTSAN
Sbjct: 241 ATLEYHHQEFSQMMAQDELRTAQFWGFDKAHHIVHGPSGPEVPPEPITTSAN 292

BLAST of MC10g0608 vs. ExPASy TrEMBL
Match: A0A5A7TRI3 (Random slug protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G005530 PE=4 SV=1)

HSP 1 Score: 540 bits (1390), Expect = 5.41e-192
Identity = 265/307 (86.32%), Postives = 284/307 (92.51%), Query Frame = 0

Query: 21  FVAVHCAPLKIDMFRLKKHSHNH---QENDSIPEDGKIDELRAALGPLSSRSLKYCTDAC 80
           ++A HCAPL IDMFR KKHS +H   Q+NDS  E+GKI+ELRAALGPLSSRS KYCTDAC
Sbjct: 16  YLAAHCAPLLIDMFRSKKHSQSHNNGQDNDSTQEEGKINELRAALGPLSSRSSKYCTDAC 75

Query: 81  LRRYLIARNWNVDKSRKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFHDRHGRS 140
           LRRYLIARNWNV+KSRKMLEETLKWRAAYKPEEIRW EVAFEGETGKVSRANF+DRHGRS
Sbjct: 76  LRRYLIARNWNVEKSRKMLEETLKWRAAYKPEEIRWDEVAFEGETGKVSRANFYDRHGRS 135

Query: 141 VLIMRPGMQNTTPSETSVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAA 200
           VLIMRPGMQNTTPSE SVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAA
Sbjct: 136 VLIMRPGMQNTTPSEASVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAA 195

Query: 201 DVINVLQNHYPERLAVSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSIELMK 260
           D+INVLQNHYPERLA + LYNPP+FFQAFWKAIKYFLDPKT QKVKFVNPKDKGS+ELMK
Sbjct: 196 DIINVLQNHYPERLAFAFLYNPPKFFQAFWKAIKYFLDPKTSQKVKFVNPKDKGSVELMK 255

Query: 261 SYFDMENLPSVFGGKATLEYHHQEFSQMMAQDELRTAQFWGFD-KAHHIVHG-PSGPEVP 320
           S+FDMENLPSVFGGKATLEY HQ+FSQMM QDEL+ AQFWGFD KAHH V+G  SGPEV 
Sbjct: 256 SHFDMENLPSVFGGKATLEYDHQQFSQMMGQDELKAAQFWGFDEKAHHSVNGHSSGPEVG 315

Query: 321 PEPITTS 322
           PEP+T++
Sbjct: 316 PEPVTST 322

BLAST of MC10g0608 vs. ExPASy TrEMBL
Match: A0A1S3BX30 (random slug protein 5 OS=Cucumis melo OX=3656 GN=LOC103494385 PE=4 SV=1)

HSP 1 Score: 521 bits (1343), Expect = 2.90e-185
Identity = 257/295 (87.12%), Postives = 274/295 (92.88%), Query Frame = 0

Query: 33  MFRLKKHSHNH---QENDSIPEDGKIDELRAALGPLSSRSLKYCTDACLRRYLIARNWNV 92
           MFR KKHS +H   Q+NDS  E+GKI+ELRAALGPLSSRS KYCTDACLRRYLIARNWNV
Sbjct: 1   MFRSKKHSQSHNNGQDNDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNV 60

Query: 93  DKSRKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFHDRHGRSVLIMRPGMQNTT 152
           +KSRKMLEETLKWRAAYKPEEIRW EVAFEGETGKVSRANF+DRHGRSVLIMRPGMQNTT
Sbjct: 61  EKSRKMLEETLKWRAAYKPEEIRWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTT 120

Query: 153 PSETSVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADVINVLQNHYPE 212
           PSE SVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAAD+INVLQNHYPE
Sbjct: 121 PSEASVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPE 180

Query: 213 RLAVSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSIELMKSYFDMENLPSVF 272
           RLA + LYNPP+FFQAFWKAIKYFLDPKT QKVKFVNPKDKGS+ELMKS+FDMENLPSVF
Sbjct: 181 RLAFAFLYNPPKFFQAFWKAIKYFLDPKTSQKVKFVNPKDKGSVELMKSHFDMENLPSVF 240

Query: 273 GGKATLEYHHQEFSQMMAQDELRTAQFWGFD-KAHHIVHG-PSGPEVPPEPITTS 322
           GGKATLEY HQ+FSQMM QDEL+ AQFWGFD KAHH V+G  SGPEV PEP+T++
Sbjct: 241 GGKATLEYDHQQFSQMMGQDELKAAQFWGFDEKAHHSVNGHSSGPEVGPEPVTST 295

BLAST of MC10g0608 vs. ExPASy TrEMBL
Match: A0A6J1J6E4 (phosphatidylinositol transfer protein 3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482999 PE=4 SV=1)

HSP 1 Score: 518 bits (1334), Expect = 6.58e-184
Identity = 257/294 (87.41%), Postives = 269/294 (91.50%), Query Frame = 0

Query: 33  MFRLKKHS--HNHQENDSIPEDGKIDELRAALGPLSSRSLKYCTDACLRRYLIARNWNVD 92
           MFRLKKHS  HNH +NDS  EDGKI ELRAALGPLS RS KYCTDACLRRYLIARNWNV+
Sbjct: 1   MFRLKKHSQSHNHHDNDSTQEDGKIIELRAALGPLSRRSSKYCTDACLRRYLIARNWNVE 60

Query: 93  KSRKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFHDRHGRSVLIMRPGMQNTTP 152
           K+RKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANF DR GRSVLIMRPGMQNTTP
Sbjct: 61  KARKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFCDRQGRSVLIMRPGMQNTTP 120

Query: 153 SETSVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADVINVLQNHYPER 212
           SE SVRHLVYLLENAILNL EGQEQMCWLIDFTGFTMKTNVSVKIA+DVIN+LQNHYPER
Sbjct: 121 SEVSVRHLVYLLENAILNLVEGQEQMCWLIDFTGFTMKTNVSVKIASDVINILQNHYPER 180

Query: 213 LAVSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSIELMKSYFDMENLPSVFG 272
           LAV+ LYNPP+ FQAFWKAIKYFLDPKT QKVKFVNPKDKGS+ELMKSYFDMENLPSVFG
Sbjct: 181 LAVAFLYNPPKIFQAFWKAIKYFLDPKTSQKVKFVNPKDKGSVELMKSYFDMENLPSVFG 240

Query: 273 GKATLEYHHQEFSQMMAQDELRTAQFWGFD-KAHHIVHGPS-GPEVPPEPITTS 322
           GKATLEY HQ+FSQMMAQDEL+ AQ W  D KAHHI +GPS GPEV PEPIT +
Sbjct: 241 GKATLEYDHQQFSQMMAQDELKAAQVWALDEKAHHITNGPSSGPEVAPEPITAT 294

BLAST of MC10g0608 vs. ExPASy TrEMBL
Match: A0A0A0KWJ1 (CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G038670 PE=4 SV=1)

HSP 1 Score: 517 bits (1332), Expect = 1.10e-183
Identity = 255/292 (87.33%), Postives = 271/292 (92.81%), Query Frame = 0

Query: 33  MFRLKKHSHNHQENDSIPEDGKIDELRAALGPLSSRSLKYCTDACLRRYLIARNWNVDKS 92
           MFR KKHS N   NDS  E+GKI+ELRAALGPLSSRS KYCTDACLRRYLIARNWNV+KS
Sbjct: 1   MFRSKKHSQN---NDSTQEEGKINELRAALGPLSSRSSKYCTDACLRRYLIARNWNVEKS 60

Query: 93  RKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFHDRHGRSVLIMRPGMQNTTPSE 152
           RKMLEETLKWRAAYKPEEI W EVAFEGETGKVSRANF+DRHGRSVLIMRPGMQNTTPSE
Sbjct: 61  RKMLEETLKWRAAYKPEEIGWDEVAFEGETGKVSRANFYDRHGRSVLIMRPGMQNTTPSE 120

Query: 153 TSVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADVINVLQNHYPERLA 212
            SVRHLVYLLENAI+NLGEGQEQMCWLIDFTGFTMKTNVSVKIAAD+INVLQNHYPERLA
Sbjct: 121 ASVRHLVYLLENAIMNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADIINVLQNHYPERLA 180

Query: 213 VSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSIELMKSYFDMENLPSVFGGK 272
            + LYNPP+FFQAFWKAIKYFLDPKTFQKVKFVNPKDKGS+ELMKS+FDMENLPSVFGGK
Sbjct: 181 FAFLYNPPKFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSVELMKSHFDMENLPSVFGGK 240

Query: 273 ATLEYHHQEFSQMMAQDELRTAQFWGFD-KAHHIVHGPS-GPEVPPEPITTS 322
           ATLEY HQ+FSQMM QDEL+ AQFWGFD KA H V+GPS GPEV PEP+T++
Sbjct: 241 ATLEYDHQQFSQMMGQDELKAAQFWGFDEKAQHSVNGPSSGPEVGPEPVTST 289

BLAST of MC10g0608 vs. TAIR 10
Match: AT1G75170.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 377.5 bits (968), Expect = 1.1e-104
Identity = 178/292 (60.96%), Postives = 228/292 (78.08%), Query Frame = 0

Query: 33  MFRLKKHSHNHQE-NDSIPEDGKIDELRAALGPLSSRSLKYCTDACLRRYLIARNWNVDK 92
           MFR K  S   QE  ++   + K+ EL+  +G LS R+  YC+DACL+RYL ARNWNV K
Sbjct: 1   MFRWKNSSQTEQEQKEAALREAKMKELKTLIGQLSGRNSLYCSDACLKRYLEARNWNVGK 60

Query: 93  SRKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFHDRHGRSVLIMRPGMQNTTPS 152
           ++KMLEETLKWR+++KPEEIRW+EV+ EGETGKV +A FHDRHGR+VLI+RPG+QNT   
Sbjct: 61  AKKMLEETLKWRSSFKPEEIRWNEVSGEGETGKVYKAGFHDRHGRTVLILRPGLQNTKSL 120

Query: 153 ETSVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADVINVLQNHYPERL 212
           E  ++HLVYL+ENAILNL E QEQM WLIDFTG++M T+V +K A + IN+LQNHYPERL
Sbjct: 121 ENQMKHLVYLIENAILNLPEDQEQMSWLIDFTGWSMSTSVPIKSARETINILQNHYPERL 180

Query: 213 AVSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSIELMKSYFDMENLPSVFGG 272
           AV+ LYNPPR F+AFWK +KYF+D KTF KVKFV PK+  S+ELM ++FD ENLP+ FGG
Sbjct: 181 AVAFLYNPPRLFEAFWKIVKYFIDAKTFVKVKFVYPKNSESVELMSTFFDEENLPTEFGG 240

Query: 273 KATLEYHHQEFSQMMAQDELRTAQFWGFDKAH----HIVHGPSGPEVPPEPI 320
           KA L+Y+++EFS+ M QD+++TA FWG   ++    H  +G SG E+ PEPI
Sbjct: 241 KALLQYNYEEFSKQMNQDDVKTANFWGLGHSNNNQLHASNGFSGAEIAPEPI 292

BLAST of MC10g0608 vs. TAIR 10
Match: AT1G75170.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 377.5 bits (968), Expect = 1.1e-104
Identity = 178/292 (60.96%), Postives = 228/292 (78.08%), Query Frame = 0

Query: 33  MFRLKKHSHNHQE-NDSIPEDGKIDELRAALGPLSSRSLKYCTDACLRRYLIARNWNVDK 92
           MFR K  S   QE  ++   + K+ EL+  +G LS R+  YC+DACL+RYL ARNWNV K
Sbjct: 1   MFRWKNSSQTEQEQKEAALREAKMKELKTLIGQLSGRNSLYCSDACLKRYLEARNWNVGK 60

Query: 93  SRKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFHDRHGRSVLIMRPGMQNTTPS 152
           ++KMLEETLKWR+++KPEEIRW+EV+ EGETGKV +A FHDRHGR+VLI+RPG+QNT   
Sbjct: 61  AKKMLEETLKWRSSFKPEEIRWNEVSGEGETGKVYKAGFHDRHGRTVLILRPGLQNTKSL 120

Query: 153 ETSVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADVINVLQNHYPERL 212
           E  ++HLVYL+ENAILNL E QEQM WLIDFTG++M T+V +K A + IN+LQNHYPERL
Sbjct: 121 ENQMKHLVYLIENAILNLPEDQEQMSWLIDFTGWSMSTSVPIKSARETINILQNHYPERL 180

Query: 213 AVSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSIELMKSYFDMENLPSVFGG 272
           AV+ LYNPPR F+AFWK +KYF+D KTF KVKFV PK+  S+ELM ++FD ENLP+ FGG
Sbjct: 181 AVAFLYNPPRLFEAFWKIVKYFIDAKTFVKVKFVYPKNSESVELMSTFFDEENLPTEFGG 240

Query: 273 KATLEYHHQEFSQMMAQDELRTAQFWGFDKAH----HIVHGPSGPEVPPEPI 320
           KA L+Y+++EFS+ M QD+++TA FWG   ++    H  +G SG E+ PEPI
Sbjct: 241 KALLQYNYEEFSKQMNQDDVKTANFWGLGHSNNNQLHASNGFSGAEIAPEPI 292

BLAST of MC10g0608 vs. TAIR 10
Match: AT4G36640.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 363.6 bits (932), Expect = 1.6e-100
Identity = 170/287 (59.23%), Postives = 230/287 (80.14%), Query Frame = 0

Query: 33  MFRLKKHSHNHQENDSIPEDGKIDELRAALGPLSSRSLKYCTDACLRRYLIARNWNVDKS 92
           MFR ++++H   +ND   +D K+ EL++A+GPLS  SL +C+DA LRR+L ARNW+V+K+
Sbjct: 1   MFR-RRNAH-QLDNDDSQQDNKVRELKSAIGPLSGHSLVFCSDASLRRFLDARNWDVEKA 60

Query: 93  RKMLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFHDRHGRSVLIMRPGMQNTTPSE 152
           +KM++ETLKWR+ YKP+EIRW++VA EGETGK SRA+FHDR GR VLIMRP MQN+T  E
Sbjct: 61  KKMIQETLKWRSTYKPQEIRWNQVAHEGETGKASRASFHDRQGRVVLIMRPAMQNSTSQE 120

Query: 153 TSVRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADVINVLQNHYPERLA 212
            ++RHLVYLLENAI+NL +GQ+QM WLIDFTG++M  N  +K   ++I++LQN+YPERL 
Sbjct: 121 GNIRHLVYLLENAIINLPKGQKQMSWLIDFTGWSMAVNPPMKTTREIIHILQNYYPERLG 180

Query: 213 VSLLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSIELMKSYFDMENLPSVFGGK 272
           ++ LYNPPR FQA ++A KYFLDP+T +KVKFV PKDK S ELM ++FD+ENLP  FGG+
Sbjct: 181 IAFLYNPPRLFQAVYRAAKYFLDPRTAEKVKFVYPKDKASDELMTTHFDVENLPKEFGGE 240

Query: 273 ATLEYHHQEFSQMMAQDELRTAQFWGFDKAHH-IVHGPSGPEVPPEP 319
           ATLEY H++FS+ M +D+L+TA++WG +  H+   +G S  +V PEP
Sbjct: 241 ATLEYDHEDFSRQMYEDDLKTAKYWGLEGKHYPKTNGFSPSDVVPEP 285

BLAST of MC10g0608 vs. TAIR 10
Match: AT4G36640.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 302.0 bits (772), Expect = 5.8e-82
Identity = 139/225 (61.78%), Postives = 182/225 (80.89%), Query Frame = 0

Query: 95  MLEETLKWRAAYKPEEIRWHEVAFEGETGKVSRANFHDRHGRSVLIMRPGMQNTTPSETS 154
           M++ETLKWR+ YKP+EIRW++VA EGETGK SRA+FHDR GR VLIMRP MQN+T  E +
Sbjct: 1   MIQETLKWRSTYKPQEIRWNQVAHEGETGKASRASFHDRQGRVVLIMRPAMQNSTSQEGN 60

Query: 155 VRHLVYLLENAILNLGEGQEQMCWLIDFTGFTMKTNVSVKIAADVINVLQNHYPERLAVS 214
           +RHLVYLLENAI+NL +GQ+QM WLIDFTG++M  N  +K   ++I++LQN+YPERL ++
Sbjct: 61  IRHLVYLLENAIINLPKGQKQMSWLIDFTGWSMAVNPPMKTTREIIHILQNYYPERLGIA 120

Query: 215 LLYNPPRFFQAFWKAIKYFLDPKTFQKVKFVNPKDKGSIELMKSYFDMENLPSVFGGKAT 274
            LYNPPR FQA ++A KYFLDP+T +KVKFV PKDK S ELM ++FD+ENLP  FGG+AT
Sbjct: 121 FLYNPPRLFQAVYRAAKYFLDPRTAEKVKFVYPKDKASDELMTTHFDVENLPKEFGGEAT 180

Query: 275 LEYHHQEFSQMMAQDELRTAQFWGFDKAHH-IVHGPSGPEVPPEP 319
           LEY H++FS+ M +D+L+TA++WG +  H+   +G S  +V PEP
Sbjct: 181 LEYDHEDFSRQMYEDDLKTAKYWGLEGKHYPKTNGFSPSDVVPEP 225

BLAST of MC10g0608 vs. TAIR 10
Match: AT1G75170.3 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 278.9 bits (712), Expect = 5.3e-75
Identity = 130/208 (62.50%), Postives = 165/208 (79.33%), Query Frame = 0

Query: 116 VAFEGETGKVSRANFHDRHGRSVLIMRPGMQNTTPSETSVRHLVYLLENAILNLGEGQEQ 175
           V+ EGETGKV +A FHDRHGR+VLI+RPG+QNT   E  ++HLVYL+ENAILNL E QEQ
Sbjct: 2   VSGEGETGKVYKAGFHDRHGRTVLILRPGLQNTKSLENQMKHLVYLIENAILNLPEDQEQ 61

Query: 176 MCWLIDFTGFTMKTNVSVKIAADVINVLQNHYPERLAVSLLYNPPRFFQAFWKAIKYFLD 235
           M WLIDFTG++M T+V +K A + IN+LQNHYPERLAV+ LYNPPR F+AFWK +KYF+D
Sbjct: 62  MSWLIDFTGWSMSTSVPIKSARETINILQNHYPERLAVAFLYNPPRLFEAFWKIVKYFID 121

Query: 236 PKTFQKVKFVNPKDKGSIELMKSYFDMENLPSVFGGKATLEYHHQEFSQMMAQDELRTAQ 295
            KTF KVKFV PK+  S+ELM ++FD ENLP+ FGGKA L+Y+++EFS+ M QD+++TA 
Sbjct: 122 AKTFVKVKFVYPKNSESVELMSTFFDEENLPTEFGGKALLQYNYEEFSKQMNQDDVKTAN 181

Query: 296 FWGFDKAH----HIVHGPSGPEVPPEPI 320
           FWG   ++    H  +G SG E+ PEPI
Sbjct: 182 FWGLGHSNNNQLHASNGFSGAEIAPEPI 209

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9UU991.1e-2936.49CRAL-TRIO domain-containing protein C23B6.04c OS=Schizosaccharomyces pombe (stra... [more]
Q55CU82.5e-2935.29Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=... [more]
P538442.7e-2332.30Phosphatidylinositol transfer protein PDR17 OS=Saccharomyces cerevisiae (strain ... [more]
P538606.0e-2331.36Phosphatidylinositol transfer protein PDR16 OS=Saccharomyces cerevisiae (strain ... [more]
O433047.3e-1323.90SEC14-like protein 5 OS=Homo sapiens OX=9606 GN=SEC14L5 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
XP_022136351.11.27e-217100.00random slug protein 5-like [Momordica charantia][more]
KAA0044746.11.12e-19186.32random slug protein 5 [Cucumis melo var. makuwa] >TYK16716.1 random slug protein... [more]
XP_038893006.13.02e-18987.71phosphatidylinositol transfer protein 3-like [Benincasa hispida] >XP_038893012.1... [more]
KAG6577254.11.46e-18784.91hypothetical protein SDJN03_24828, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023553217.14.07e-18587.76phosphatidylinositol transfer protein 3-like isoform X1 [Cucurbita pepo subsp. p... [more]
Match NameE-valueIdentityDescription
A0A6J1C3A46.13e-218100.00random slug protein 5-like OS=Momordica charantia OX=3673 GN=LOC111008083 PE=4 S... [more]
A0A5A7TRI35.41e-19286.32Random slug protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21... [more]
A0A1S3BX302.90e-18587.12random slug protein 5 OS=Cucumis melo OX=3656 GN=LOC103494385 PE=4 SV=1[more]
A0A6J1J6E46.58e-18487.41phosphatidylinositol transfer protein 3-like isoform X1 OS=Cucurbita maxima OX=3... [more]
A0A0A0KWJ11.10e-18387.33CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G038670 P... [more]
Match NameE-valueIdentityDescription
AT1G75170.11.1e-10460.96Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G75170.21.1e-10460.96Sec14p-like phosphatidylinositol transfer family protein [more]
AT4G36640.11.6e-10059.23Sec14p-like phosphatidylinositol transfer family protein [more]
AT4G36640.25.8e-8261.78Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G75170.35.3e-7562.50Sec14p-like phosphatidylinositol transfer family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001251CRAL-TRIO lipid binding domainSMARTSM00516sec14_4coord: 119..274
e-value: 3.7E-45
score: 166.0
IPR001251CRAL-TRIO lipid binding domainPFAMPF00650CRAL_TRIOcoord: 122..272
e-value: 3.6E-35
score: 121.0
IPR001251CRAL-TRIO lipid binding domainPROSITEPS50191CRAL_TRIOcoord: 132..277
score: 20.460461
IPR001251CRAL-TRIO lipid binding domainCDDcd00170SEC14coord: 131..272
e-value: 2.71347E-37
score: 129.042
IPR011074CRAL/TRIO, N-terminal domainSMARTSM01100CRAL_TRIO_N_2coord: 74..99
e-value: 3.0E-7
score: 40.1
IPR011074CRAL/TRIO, N-terminal domainPFAMPF03765CRAL_TRIO_Ncoord: 73..98
e-value: 3.0E-6
score: 27.3
IPR036865CRAL-TRIO lipid binding domain superfamilyGENE3D3.40.525.10coord: 31..306
e-value: 5.0E-79
score: 267.6
IPR036865CRAL-TRIO lipid binding domain superfamilySUPERFAMILY52087CRAL/TRIO domaincoord: 113..284
NoneNo IPR availablePANTHERPTHR45824GH16843Pcoord: 37..321
NoneNo IPR availablePANTHERPTHR45824:SF17DIVERGENT CRAL/TRIO DOMAIN PROTEINcoord: 37..321
IPR036273CRAL/TRIO, N-terminal domain superfamilySUPERFAMILY46938CRAL/TRIO N-terminal domaincoord: 41..115

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g0608.1MC10g0608.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0120009 intermembrane lipid transfer
biological_process GO:0015914 phospholipid transport
cellular_component GO:0016020 membrane
molecular_function GO:0008526 phosphatidylinositol transfer activity