MC10g0591 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g0591
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionDNA-directed RNA polymerases II and V subunit 8A-like
LocationMC10: 4884941 .. 4887853 (-)
RNA-Seq ExpressionMC10g0591
SyntenyMC10g0591
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGATTTACATTATACGCAACAGAAGCACCTGAAGTACTAAACAGAATAGGTATTAGTTTGGTAATGGTTGGAGCGGCAAACTCGGATTCGGCATGAGCCGGCGAGCCTCCAGGATAAGGGAGCGGACACTACTAACCAAGCCAAGCCTAGTTCTCGCCGATAGGCGCTGGTAGCTTGCTTCCAAGCCTTGCCAACTATAATAAATAGTTGTGGCCACGGCCCCAAGGAGCCTTAAGCACTTGTACAATGCTCAAGTCGACGGCTCCATCCTACTCGTTGCCCAGAGCCGTCGACTTTCGAAACTATTAGTAACAAGAATATCTTAGAAATAATATCTTGAAATTGTGAAGATTGGTTTTTTTTTTCTTTTTTTCTTTTTTTTGAAATCAATGTGAAGATTGTTGTTATATTATTGGTTTAATCATTTTCTCATTAGTTATTGTTTTATTAAGCCTTTTTTAAAAAAAAATTAAGCCTTTTTTTTTATAATTAGTTTGCTTCCCTATGATTCATGTTGGGCCAATTTCTTTTGTATTATGTTTTATGGCCCAATAGAAGACAACTCTTCTTCTCTCTTCTCCATCCCAAAACCCTACACATAATTTTGAATATTTTGTGTTGAGAAAAACCCTAATTAGAAATTGGAGAGCCACCCACCTTGAGAGAGAGATTCCGGATTTTTCCAGTCGTCTGACGCCGCTGCTCGCCGTCCGCCACCAGAAGCCGCCGGACGCCTCTAGAAGCTGCACGCCGTCGCCATCGGAAGCCGCCGCCGCCGGAGTGGGCTGGGCAAATTATCCATATTTTATAATTACATAGAATCTCAACACTCTCCATATTTGATATATTTTTTTGTTGCTTTTCTATCGGTGTAATTCTGCATTTTGAGGAGCAAATTATCCACAAACATGGTGGAGCTTCTATTTGATGATATTTTCAAGGTCAAGTGTGTCAACCCAGATGGGCAAAAGTATGATAAAGGTACTTTAATTTTCTGGTTTTTGAAACTCTCAATTTATGCGCTGTCTTGACTGGCTGTGAACGATTCTAGATGAGTGGCGATTGACTGCATAAATTTGTTCCTTTTAGTTTCTAGAATTGACGCAAGGAGTGAGAAACTTGCCATGCATATGCTTCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGTGAGAAGATTCTGATGGTCTTATCTCCCACACTAAATTATGATGGAGCTCCTGTTGCCAGTCATAGTAGTCAGGTAATATTTTCCCCCTTAATATTTAAGAAAACAACATATAGAAATTAAATTTCCTGGCCATATCTGAATCTATATTCATAGAAATGTTAGAAAGGTAATTCCATAAACTTTCTCTGAAACTGAAAGTCTGAAAATCTAGAAGAGGATTTTGCCCAGCGGCCTCAAGAAACTAGAAAATAGGGTCACAATGTGCAAGTCGAGCATGTTCTTCAGTTCAGGCTGGATAGTCATGTCCCTAGAAAATAAAAAAGAAAAAGGGGATAGGCACTTATTTCAAGTCTTTCAGTGTCTTTCTAGGCTCAATGTGTTGGAGGAGGTGCTTGTTAATAATCCCCCTCTCCTGAAAAAAGGGATCAGGTATTTGGCACGCGGGTTTCTTTGCTATCTCATGGAATTTATGGCTCAGAAGAAATTGAAGAGTTTTTAGAGATATATAGAAGTCCTAGGTGGAAGTTTTGAATGCGATGCATTTCAATGCCACTCACTAGGTGCCCTTTCTGAAGTGTTCTTAATGATTGGAACAATCTCTTATAGTTTCCTTTCTCTTTACCTTTTTTTTTTTTTTGAGAAGAAACTAGAATTTTATTGAGGAATGAAATGAACAAGACAAGGGAAGAACCAAGAGCCATAATGGAGTTGCAAACTGTTTGCTACTTTTTTTTGGACTGCTCTTTTTTGTAATACGCTCTTTATGTTCCTTCATTTGTTCTGGTGGAAGTTAATTTATTAAGAAAAATACATGTTTTTCTTCTTCTTAGTATGGGATTTGGGAGGTACCATGACAATAAATGGATAAAAAAGAGGACGTGTTTTTGACGAGAACCGCTTTCACAAGCCTGTTGTACTCTGTCAAGATTGTGTACATAATTGTAATCTCATTGAGATTGAAGTAATATCTTTCAAATAATTATTTTTTGCAGGAAGGGAAGAAATCATTAGCAGATAAGTTTGAGTATGTGATGCATGGGAAACTATACAAACTTTCAGAAGAAGGCTCAGGATCAGACTTGAAAGTGTAAGTGAACTAGCATTGCCATGATTTTGCTCAACAATTTTGATCTGCTAATCCCTAAATCTACAATTTAGTCCCGTATCCGCCGTTTTCAATTTTGCCTCTTATCTTTGATGCCAAACGTTAAAAGAAGGGGGTTATGAGTAAATTTGAAAATGATAGTTTTAAACCAACCAATTTGGGTCAGATAAGTTACTACAAATCAGATCATCATATAAAGATATGCTTATTTCTAAAAATCGAAATGTAGCGTATTTTCAACTCGATTAATAATGTATATATTTTAACGATGTTTGGTTGGAATGGTTCTGTTTTCAGAGAGGTTTATGCATCTTTTGGAGGACTCCAAATGATGTTGAAGGGCGATCCTGTCCACTGTGCCAAATTCAGGGTAGATCAGAACATGTTTTTGCTCCTTCGGAAGCTCCCATGAACTGCAACCATCACTCCACTTCTTCACTCTCATGGCCTTTGTTTTTACAGAAATTATTATCTATGGCCTGATTATTTATTATATTTACAGATATCTTGTGAATTGAAGATCTTTACTAAATTGATTTTCCTAGTTTGTTGGATGGTTTAACATAACAAGTGTTCTTTTTCCCTTCCTACTTTCTAATTTCTTGAGATTATAAATTCTCACGTGGGAGCTA

mRNA sequence

GGGATTTACATTATACGCAACAGAAGCACCTGAAGTACTAAACAGAATAGGTATTAGTTTGGTAATGGTTGGAGCGGCAAACTCGGATTCGGCATGAGCCGGCGAGCCTCCAGGATAAGGGAGCGGACACTACTAACCAAGCCAAGCCTAGTTCTCGCCGATAGGCGCTGGTAGCTTGCTTCCAAGCCTTGCCAACTATAATAAATAGTTGTGGCCACGGCCCCAAGGAGCCTTAAGCACTTGTACAATGCTCAAGTCGACGGCTCCATCCTACTCGTTGCCCAGAGCCGTCGACTTTCGAAACTATTAGTAACAAGAATATCTTAGAAATAATATCTTGAAATTGTGAAGATTGGTTTTTTTTTTCTTTTTTTCTTTTTTTTGAAATCAATGTGAAGATTGTTGTTATATTATTGGTTTAATCATTTTCTCATTAGTTATTGTTTTATTAAGCCTTTTTTAAAAAAAAATTAAGCCTTTTTTTTTATAATTAGTTTGCTTCCCTATGATTCATGTTGGGCCAATTTCTTTTGTATTATGTTTTATGGCCCAATAGAAGACAACTCTTCTTCTCTCTTCTCCATCCCAAAACCCTACACATAATTTTGAATATTTTGTGTTGAGAAAAACCCTAATTAGAAATTGGAGAGCCACCCACCTTGAGAGAGAGATTCCGGATTTTTCCAGTCGTCTGACGCCGCTGCTCGCCGTCCGCCACCAGAAGCCGCCGGACGCCTCTAGAAGCTGCACGCCGTCGCCATCGGAAGCCGCCGCCGCCGGAGTGGGCTGGGCAAATTATCCATATTTTATAATTACATAGAATCTCAACACTCTCCATATTTGATATATTTTTTTGTTGCTTTTCTATCGGTGTAATTCTGCATTTTGAGGAGCAAATTATCCACAAACATGGTGGAGCTTCTATTTGATGATATTTTCAAGGTCAAGTGTGTCAACCCAGATGGGCAAAAGTATGATAAAGTTTCTAGAATTGACGCAAGGAGTGAGAAACTTGCCATGCATATGCTTCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGTGAGAAGATTCTGATGGTCTTATCTCCCACACTAAATTATGATGGAGCTCCTGTTGCCAGTCATAGTAGTCAGGAAGGGAAGAAATCATTAGCAGATAAGTTTGAGTATGTGATGCATGGGAAACTATACAAACTTTCAGAAGAAGGCTCAGGATCAGACTTGAAAGTAGAGGTTTATGCATCTTTTGGAGGACTCCAAATGATGTTGAAGGGCGATCCTGTCCACTGTGCCAAATTCAGGGTAGATCAGAACATGTTTTTGCTCCTTCGGAAGCTCCCATGAACTGCAACCATCACTCCACTTCTTCACTCTCATGGCCTTTGTTTTTACAGAAATTATTATCTATGGCCTGATTATTTATTATATTTACAGATATCTTGTGAATTGAAGATCTTTACTAAATTGATTTTCCTAGTTTGTTGGATGGTTTAACATAACAAGTGTTCTTTTTCCCTTCCTACTTTCTAATTTCTTGAGATTATAAATTCTCACGTGGGAGCTA

Coding sequence (CDS)

ATGGTGGAGCTTCTATTTGATGATATTTTCAAGGTCAAGTGTGTCAACCCAGATGGGCAAAAGTATGATAAAGTTTCTAGAATTGACGCAAGGAGTGAGAAACTTGCCATGCATATGCTTCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGTGAGAAGATTCTGATGGTCTTATCTCCCACACTAAATTATGATGGAGCTCCTGTTGCCAGTCATAGTAGTCAGGAAGGGAAGAAATCATTAGCAGATAAGTTTGAGTATGTGATGCATGGGAAACTATACAAACTTTCAGAAGAAGGCTCAGGATCAGACTTGAAAGTAGAGGTTTATGCATCTTTTGGAGGACTCCAAATGATGTTGAAGGGCGATCCTGTCCACTGTGCCAAATTCAGGGTAGATCAGAACATGTTTTTGCTCCTTCGGAAGCTCCCATGA

Protein sequence

MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVLSPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQMMLKGDPVHCAKFRVDQNMFLLLRKLP
Homology
[Close]
BLAST of MC10g0591 vs. ExPASy Swiss-Prot
Match: O81097 (DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702 GN=NRPB8A PE=1 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 6.4e-41
Identity = 79/142 (55.63%), Postives = 104/142 (73.24%), Query Frame = 0 

Query: 4   LLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVLSPT 63
           +LF+DIF V  ++PDG+K+DKV+R+ A S  L M M LDVNTE+YP+  G+K  + L+PT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P   + +   KK+LADK+EY+MHGKLYK+SE   G   K E+Y SFGGL M+LK
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISER-DGKTPKAELYVSFGGLLMLLK 125

Query: 124 GDPVHCAKFRVDQNMFLLLRKL 146
           GDP H + F +DQ +FLL+RKL
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]
BLAST of MC10g0591 vs. ExPASy Swiss-Prot
Match: Q9M1A8 (DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=3702 GN=NRPB8B PE=1 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 6.4e-41
Identity = 77/142 (54.23%), Postives = 106/142 (74.65%), Query Frame = 0 

Query: 4   LLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVLSPT 63
           ++F+DIF V  ++PDG+K+DKV+R++ARS  L M M LDVNTE+YP+  G+K  + ++PT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P   + +   KK+LADK+EY+MHGKLYK+SE   G   K E+Y SFGGL M+L+
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISER-DGKTPKAELYVSFGGLLMLLQ 125

Query: 124 GDPVHCAKFRVDQNMFLLLRKL 146
           GDP H + F +DQ +FLL+RKL
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]
BLAST of MC10g0591 vs. ExPASy Swiss-Prot
Match: Q19826 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis elegans OX=6239 GN=rpb-8 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 3.0e-30
Identity = 60/145 (41.38%), Postives = 99/145 (68.28%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL 60
           M  ++FDD+FKVK V+PDG+K+D+VSR    +E   M +++D+N++IYP+ + +K+ +VL
Sbjct: 1   MAGIIFDDMFKVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKVRLVL 60

Query: 61  SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSD-LKVEVYASFGGLQ 120
           + TL  DG         + +     ++EYVM+GK+Y+L ++ +G+D  K+  YASFGGL 
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDTGTDGGKLAAYASFGGLL 120

Query: 121 MMLKGDPVHCAKFRVDQNMFLLLRK 145
           M LKG+ ++   F VD N++LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

[Close]
BLAST of MC10g0591 vs. ExPASy Swiss-Prot
Match: A8XT81 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis briggsae OX=6238 GN=rpb-8 PE=3 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 3.3e-29
Identity = 59/145 (40.69%), Postives = 97/145 (66.90%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL 60
           M  ++FDD+F VK V+PDG+K+D+VSR    +E   M +++D+N++IYP+ + +K  +VL
Sbjct: 1   MAGIIFDDMFSVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKFRLVL 60

Query: 61  SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSD-LKVEVYASFGGLQ 120
           + TL  DG         + +     ++EYVM+GK+Y+L ++ SG+D  ++  YASFGGL 
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDSGTDGGQLAAYASFGGLL 120

Query: 121 MMLKGDPVHCAKFRVDQNMFLLLRK 145
           M LKG+ ++   F VD N++LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

[Close]
BLAST of MC10g0591 vs. ExPASy Swiss-Prot
Match: Q54YW8 (DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium discoideum OX=44689 GN=polr2h PE=3 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 4.3e-29
Identity = 65/144 (45.14%), Postives = 97/144 (67.36%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL 60
           M  +LF+D F+VK ++PDG+K+D+VSR    SE   M + +D+ T IYP+   E+  MVL
Sbjct: 1   MTSILFEDTFEVKEIDPDGKKFDRVSRFVCYSENYEMDLQIDIATHIYPLGH-ERFKMVL 60

Query: 61  SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQM 120
           + +LN DG+   S S    K SLADK++YVM+GK++K  +E S S  KV V+ASFGGL +
Sbjct: 61  ASSLNLDGSSDNSGSYDTNKPSLADKYDYVMYGKIFKYQKENSSS--KVSVFASFGGLLV 120

Query: 121 MLKGDPVHCAKFRVDQNMFLLLRK 145
           +L+GDP +     +D  ++LL++K
Sbjct: 121 LLQGDPRYLPGIELDARIYLLIKK 141

[Close]
BLAST of MC10g0591 vs. NCBI nr
Match: XP_022136431.1 (DNA-directed RNA polymerases II and V subunit 8A-like [Momordica charantia])

HSP 1 Score: 295 bits (756), Expect = 6.46e-101
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL 60
           MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL
Sbjct: 1   MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPVHCAKFRVDQNMFLLLRKLP 146
           MLKGDPVHCAKFRVDQNMFLLLRKLP
Sbjct: 121 MLKGDPVHCAKFRVDQNMFLLLRKLP 146

[Close]
BLAST of MC10g0591 vs. NCBI nr
Match: XP_022929200.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >XP_022929202.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >XP_022985172.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_023551974.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita pepo subsp. pepo] >KAG6577274.1 DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015358.1 DNA-directed RNA polymerases II, IV and V subunit 8B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 273 bits (698), Expect = 4.38e-92
Identity = 130/145 (89.66%), Postives = 142/145 (97.93%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL 60
           M ELLF+D+FKVKCVNPDG+KYDKVSRI+ARSEKL+M+MLLDVNTEIYPIDEGEKILMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPV S+S QEGKKSLADKFEY+MHGK+YKLS+EGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPVHCAKFRVDQNMFLLLRKL 145
           +LKGDP+HCAKFRVDQNMFLLLRKL
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

[Close]
BLAST of MC10g0591 vs. NCBI nr
Match: XP_022983196.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983203.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983212.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983221.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983229.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima])

HSP 1 Score: 273 bits (698), Expect = 4.53e-92
Identity = 131/145 (90.34%), Postives = 142/145 (97.93%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL 60
           M E+LFDDIFKVKCVNPDG+KYDKVSRI+ARSEKL+M+MLLDVNTEIYPIDEG+KILMVL
Sbjct: 1   MAEVLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPV S+S QEGKKSLADKFEY+MHGKLYKL++EGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPVHCAKFRVDQNMFLLLRKL 145
           MLKGDP+HCAKFRVDQNMFLLLRKL
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKL 145

[Close]
BLAST of MC10g0591 vs. NCBI nr
Match: KAG6600626.1 (DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031262.1 DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 271 bits (693), Expect = 2.62e-91
Identity = 130/145 (89.66%), Postives = 141/145 (97.24%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL 60
           M ELLFDDIFKVKCVNPDG+KYDKVSRI+ARSEKL+M+MLLDVNTEIYPIDEG+KILMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPV  +S Q+GKKSLADKFEY+MHGKLYKL++EGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPVHCAKFRVDQNMFLLLRKL 145
           MLKGDP+HCAKFRVDQNMFLLLRKL
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKL 145

[Close]
BLAST of MC10g0591 vs. NCBI nr
Match: XP_022942165.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata])

HSP 1 Score: 270 bits (689), Expect = 1.07e-90
Identity = 130/145 (89.66%), Postives = 140/145 (96.55%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL 60
           M ELLFDDIFKVKCVNPDG+KYDKVSRI+ARSEKL+M+MLLDVNTEIYPIDEG+KILMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPV S+S QEGKKSLADKFEY+MH KLYKL++EGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHRKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPVHCAKFRVDQNMFLLLRKL 145
           MLKGDP+HC KFRVDQNMFLLLRKL
Sbjct: 121 MLKGDPLHCVKFRVDQNMFLLLRKL 145

[Close]
BLAST of MC10g0591 vs. ExPASy TrEMBL
Match: A0A6J1C3H4 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Momordica charantia OX=3673 GN=LOC111008140 PE=3 SV=1)

HSP 1 Score: 295 bits (756), Expect = 3.13e-101
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL 60
           MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL
Sbjct: 1   MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPVHCAKFRVDQNMFLLLRKLP 146
           MLKGDPVHCAKFRVDQNMFLLLRKLP
Sbjct: 121 MLKGDPVHCAKFRVDQNMFLLLRKLP 146

[Close]
BLAST of MC10g0591 vs. ExPASy TrEMBL
Match: A0A6J1ETP8 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata OX=3662 GN=LOC111435865 PE=3 SV=1)

HSP 1 Score: 273 bits (698), Expect = 2.12e-92
Identity = 130/145 (89.66%), Postives = 142/145 (97.93%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL 60
           M ELLF+D+FKVKCVNPDG+KYDKVSRI+ARSEKL+M+MLLDVNTEIYPIDEGEKILMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPV S+S QEGKKSLADKFEY+MHGK+YKLS+EGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPVHCAKFRVDQNMFLLLRKL 145
           +LKGDP+HCAKFRVDQNMFLLLRKL
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

[Close]
BLAST of MC10g0591 vs. ExPASy TrEMBL
Match: A0A6J1J454 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX=3661 GN=LOC111483257 PE=3 SV=1)

HSP 1 Score: 273 bits (698), Expect = 2.12e-92
Identity = 130/145 (89.66%), Postives = 142/145 (97.93%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL 60
           M ELLF+D+FKVKCVNPDG+KYDKVSRI+ARSEKL+M+MLLDVNTEIYPIDEGEKILMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPV S+S QEGKKSLADKFEY+MHGK+YKLS+EGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPVHCAKFRVDQNMFLLLRKL 145
           +LKGDP+HCAKFRVDQNMFLLLRKL
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

[Close]
BLAST of MC10g0591 vs. ExPASy TrEMBL
Match: A0A6J1J6Q9 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX=3661 GN=LOC111481850 PE=3 SV=1)

HSP 1 Score: 273 bits (698), Expect = 2.19e-92
Identity = 131/145 (90.34%), Postives = 142/145 (97.93%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL 60
           M E+LFDDIFKVKCVNPDG+KYDKVSRI+ARSEKL+M+MLLDVNTEIYPIDEG+KILMVL
Sbjct: 1   MAEVLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPV S+S QEGKKSLADKFEY+MHGKLYKL++EGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPVHCAKFRVDQNMFLLLRKL 145
           MLKGDP+HCAKFRVDQNMFLLLRKL
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKL 145

[Close]
BLAST of MC10g0591 vs. ExPASy TrEMBL
Match: A0A6J1FVR2 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata OX=3662 GN=LOC111447307 PE=3 SV=1)

HSP 1 Score: 270 bits (689), Expect = 5.17e-91
Identity = 130/145 (89.66%), Postives = 140/145 (96.55%), Query Frame = 0 

Query: 1   MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL 60
           M ELLFDDIFKVKCVNPDG+KYDKVSRI+ARSEKL+M+MLLDVNTEIYPIDEG+KILMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPV S+S QEGKKSLADKFEY+MH KLYKL++EGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHRKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPVHCAKFRVDQNMFLLLRKL 145
           MLKGDP+HC KFRVDQNMFLLLRKL
Sbjct: 121 MLKGDPLHCVKFRVDQNMFLLLRKL 145

[Close]
BLAST of MC10g0591 vs. TAIR 10
Match: AT1G54250.1 (RNA polymerase Rpb8 )

HSP 1 Score: 168.3 bits (425), Expect = 4.5e-42
Identity = 79/142 (55.63%), Postives = 104/142 (73.24%), Query Frame = 0 

Query: 4   LLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVLSPT 63
           +LF+DIF V  ++PDG+K+DKV+R+ A S  L M M LDVNTE+YP+  G+K  + L+PT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P   + +   KK+LADK+EY+MHGKLYK+SE   G   K E+Y SFGGL M+LK
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISER-DGKTPKAELYVSFGGLLMLLK 125

Query: 124 GDPVHCAKFRVDQNMFLLLRKL 146
           GDP H + F +DQ +FLL+RKL
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]
BLAST of MC10g0591 vs. TAIR 10
Match: AT3G59600.1 (RNA polymerase Rpb8 )

HSP 1 Score: 168.3 bits (425), Expect = 4.5e-42
Identity = 77/142 (54.23%), Postives = 106/142 (74.65%), Query Frame = 0 

Query: 4   LLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVLSPT 63
           ++F+DIF V  ++PDG+K+DKV+R++ARS  L M M LDVNTE+YP+  G+K  + ++PT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P   + +   KK+LADK+EY+MHGKLYK+SE   G   K E+Y SFGGL M+L+
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISER-DGKTPKAELYVSFGGLLMLLQ 125

Query: 124 GDPVHCAKFRVDQNMFLLLRKL 146
           GDP H + F +DQ +FLL+RKL
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

The following BLAST results are available for this feature:
BLAST of MC10g0591 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O810976.4e-4155.63DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702... [more]
Q9M1A86.4e-4154.23DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=... [more]
Q198263.0e-3041.38Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab... [more]
A8XT813.3e-2940.69Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab... [more]
Q54YW84.3e-2945.14DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium disc... [more]
BLAST of MC10g0591 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022136431.16.46e-101100.00DNA-directed RNA polymerases II and V subunit 8A-like [Momordica charantia][more]
XP_022929200.14.38e-9289.66DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >... [more]
XP_022983196.14.53e-9290.34DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP... [more]
KAG6600626.12.62e-9189.66DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyros... [more]
XP_022942165.11.07e-9089.66DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata][more]
BLAST of MC10g0591 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1C3H43.13e-101100.00DNA-directed RNA polymerases II and V subunit 8A-like OS=Momordica charantia OX=... [more]
A0A6J1ETP82.12e-9289.66DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata ... [more]
A0A6J1J4542.12e-9289.66DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX... [more]
A0A6J1J6Q92.19e-9290.34DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX... [more]
A0A6J1FVR25.17e-9189.66DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata ... [more]
BLAST of MC10g0591 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G54250.14.5e-4255.63RNA polymerase Rpb8 [more]
AT3G59600.14.5e-4254.23RNA polymerase Rpb8 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005570RNA polymerase, Rpb8SMARTSM00658rpol8neucoord: 2..144
e-value: 1.1E-55
score: 201.0
IPR005570RNA polymerase, Rpb8PFAMPF03870RNA_pol_Rpb8coord: 7..144
e-value: 2.4E-50
score: 170.3
IPR005570RNA polymerase, Rpb8PIRSFPIRSF000779RPB8coord: 1..146
e-value: 8.5E-48
score: 160.0
IPR005570RNA polymerase, Rpb8PANTHERPTHR10917DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3coord: 1..145
NoneNo IPR availableGENE3D2.40.50.140coord: 6..146
e-value: 2.7E-54
score: 185.1
NoneNo IPR availablePANTHERPTHR10917:SF1DNA-DIRECTED RNA POLYMERASE I, IIcoord: 1..145
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 4..144

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g0591.1MC10g0591.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005736 RNA polymerase I complex
cellular_component GO:0005665 RNA polymerase II, core complex
cellular_component GO:0005666 RNA polymerase III complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity