MC10g0426 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g0426
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	E3 ubiquitin-protein ligase
Location	MC10: 3460107 .. 3460883 (+)
RNA-Seq Expression	MC10g0426
Synteny	MC10g0426

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCAGAACCTTCGACCTCGGATCGCCATCAATGGCGGCGTCCACAGGACGACAACTCTTCATTATTACTGGTGTCGAATCTGTCGACGAATTATCAGGATCAGCTTCGGAAATCCCCTCGAAATTTCGATTTCTTGCCCCTTCTGCTCCAGGCTTCTCCGCCACGAACTCGACGTAGCTAGGGCTCGATTCCACGCCGATCCTTCTGGTTTTCTCCCAAATTCCTCTCGCCTCCCCGATCCACGTCCCGATTCTCGCCGGAATTTACCTCTCTGGGGTTTTGAAATCGACGACGGCCCTTCTCCAGAGTCGTGGATTACTCTCCAATTCTCCCGACCCCGAGCCCTCCCCGAAAGTTCCGATGCGCCTCCCGATTCGAGGCAGGATTGCGGAATCGAAGCGATTCCGAGGGTGAAAATTACAGAGTACCATTTGGGGAAGGATTCGATTTGTCCAATTTGTAAAGAGGAATTTAGAATCGGAGGAGAAGCGAGAGAATTACCTTGCAAGCACTTTTACCATTCGGATTGTATTGTTCCGTGGTTGAGGCTCCGCAATACTTGCCCTGTTTGCCGCCATACGCTGCAGAACATCCCCGTCGCCGTCGCCGTCGCCGCCGCCGGGACTTTTGTCGAAGAAGGAGGCGGAAGGTGGAATGGATGGTGGAGCTCAATCTGTTCGTGGTGGCCGTTCCGTTCAATTGGTGGCTGGGCTCGCCGACTCCGGTATGCTGATTCCGGGGATAGCTTCTTCGTCATTGAACGTAAGATTTTG

mRNA sequence

ATGTCTCAGAACCTTCGACCTCGGATCGCCATCAATGGCGGCGTCCACAGGACGACAACTCTTCATTATTACTGGTGTCGAATCTGTCGACGAATTATCAGGATCAGCTTCGGAAATCCCCTCGAAATTTCGATTTCTTGCCCCTTCTGCTCCAGGCTTCTCCGCCACGAACTCGACGTAGCTAGGGCTCGATTCCACGCCGATCCTTCTGGTTTTCTCCCAAATTCCTCTCGCCTCCCCGATCCACGTCCCGATTCTCGCCGGAATTTACCTCTCTGGGGTTTTGAAATCGACGACGGCCCTTCTCCAGAGTCGTGGATTACTCTCCAATTCTCCCGACCCCGAGCCCTCCCCGAAAGTTCCGATGCGCCTCCCGATTCGAGGCAGGATTGCGGAATCGAAGCGATTCCGAGGGTGAAAATTACAGAGTACCATTTGGGGAAGGATTCGATTTGTCCAATTTGTAAAGAGGAATTTAGAATCGGAGGAGAAGCGAGAGAATTACCTTGCAAGCACTTTTACCATTCGGATTGTATTGTTCCGTGGTTGAGGCTCCGCAATACTTGCCCTGTTTGCCGCCATACGCTGCAGAACATCCCCGTCGCCGTCGCCGTCGCCGCCGCCGGGACTTTTGTCGAAGAAGGAGGCGGAAGGTGGAATGGATGGTGGAGCTCAATCTGTTCGTGGTGGCCGTTCCGTTCAATTGGTGGCTGGGCTCGCCGACTCCGGTATGCTGATTCCGGGGATAGCTTCTTCGTCATTGAACGTAAGATTTTG

Coding sequence (CDS)

ATGTCTCAGAACCTTCGACCTCGGATCGCCATCAATGGCGGCGTCCACAGGACGACAACTCTTCATTATTACTGGTGTCGAATCTGTCGACGAATTATCAGGATCAGCTTCGGAAATCCCCTCGAAATTTCGATTTCTTGCCCCTTCTGCTCCAGGCTTCTCCGCCACGAACTCGACGTAGCTAGGGCTCGATTCCACGCCGATCCTTCTGGTTTTCTCCCAAATTCCTCTCGCCTCCCCGATCCACGTCCCGATTCTCGCCGGAATTTACCTCTCTGGGGTTTTGAAATCGACGACGGCCCTTCTCCAGAGTCGTGGATTACTCTCCAATTCTCCCGACCCCGAGCCCTCCCCGAAAGTTCCGATGCGCCTCCCGATTCGAGGCAGGATTGCGGAATCGAAGCGATTCCGAGGGTGAAAATTACAGAGTACCATTTGGGGAAGGATTCGATTTGTCCAATTTGTAAAGAGGAATTTAGAATCGGAGGAGAAGCGAGAGAATTACCTTGCAAGCACTTTTACCATTCGGATTGTATTGTTCCGTGGTTGAGGCTCCGCAATACTTGCCCTGTTTGCCGCCATACGCTGCAGAACATCCCCGTCGCCGTCGCCGTCGCCGCCGCCGGGACTTTTGTCGAAGAAGGAGGCGGAAGGTGGAATGGATGGTGGAGCTCAATCTGTTCGTGGTGGCCGTTCCGTTCAATTGGTGGCTGGGCTCGCCGACTCCGGTATGCTGATTCCGGGGATAGCTTCTTCGTCATTGAACGTAAGATTTTG

Protein sequence

MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDVARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPESSDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIVPWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWARRLRYADSGDSFFVIERKIL

Homology

[Close]

BLAST of MC10g0426 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 7.6e-21
Identity = 53/125 (42.40%), Postives = 68/125 (54.40%), Query Frame = 0


Query: 124 PPDSRQDCGIEAIPRVKITEYHL-GKDSICPICKEEFRIGGEARELPCKHFYHSDCIVPW 183
           PP  R    I+A+P +KI + HL   DS CP+CK+EF +G EA+++PC H YHSDCIVPW
Sbjct: 163 PPAPRS--AIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPW 222

Query: 184 LRLRNTCPVCRHTL---------QNIPVAVAVAAAGTFVEEGGGRWNG--WWSSICSWWP 237
           L   N+CPVCR  L         QN         + +       R NG    +   S+WP
Sbjct: 223 LVQHNSCPVCRQELPSASGPSSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWP 282

[Close]

BLAST of MC10g0426 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 4.9e-20
Identity = 41/80 (51.25%), Postives = 56/80 (70.00%), Query Frame = 0


Query: 121 SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV 180
           S  PP S+    IE++PRV+I++ H+G ++ C +C E F    EARE+PCKH +H DCIV
Sbjct: 170 SGNPPASKS--AIESLPRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHLFHDDCIV 229

Query: 181 PWLRLRNTCPVCRHTLQNIP 201
           PWL +RN+CPVCR  L + P
Sbjct: 230 PWLSIRNSCPVCRFELPSEP 247

[Close]

BLAST of MC10g0426 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.1e-19
Identity = 49/112 (43.75%), Postives = 66/112 (58.93%), Query Frame = 0


Query: 125 PDSRQDCGIEAIPRVKITEYHL-GKDSICPICKEEFRIGGEARELPCKHFYHSDCIVPWL 184
           P       I+A+P +KIT+ HL   DS CP+CK+EF +  EA+++PC H YHSDCIVPWL
Sbjct: 158 PPPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWL 217

Query: 185 RLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICS-WWPFRS 235
              N+CPVCR  L +   + +  ++        GR N    +I S  WPFRS
Sbjct: 218 VQHNSCPVCRKELPSRGSSSSTQSSQN--RSTNGRENSRRRNIFSNLWPFRS 267

[Close]

BLAST of MC10g0426 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 2.5e-19
Identity = 40/81 (49.38%), Postives = 55/81 (67.90%), Query Frame = 0


Query: 121 SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV 180
           S  PP S+    IE++PRV+I++ H   ++ C +C E F  G E RE+PCKH +H DCIV
Sbjct: 186 SGNPPASKS--AIESLPRVEISDCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIV 245

Query: 181 PWLRLRNTCPVCRHTLQNIPV 202
           PWL +RN+CPVCR  L + P+
Sbjct: 246 PWLSIRNSCPVCRFELPSDPI 264

[Close]

BLAST of MC10g0426 vs. ExPASy Swiss-Prot
Match: Q6DIP3 (E3 ubiquitin-protein ligase RNF126 OS=Xenopus tropicalis OX=8364 GN=rnf126 PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 9.3e-19
Identity = 36/78 (46.15%), Postives = 54/78 (69.23%), Query Frame = 0


Query: 119 ESSDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDC 178
           E++  PP   +   I+A+P ++ITE H+G    CP+CKE++ +G   R+LPC H +H+DC
Sbjct: 196 ENTGPPPADTEK--IQALPTIQITEEHVGSGLECPVCKEDYTVGESVRQLPCNHLFHNDC 255

Query: 179 IVPWLRLRNTCPVCRHTL 197
           I+PWL   +TCPVCR +L
Sbjct: 256 IIPWLEQHDTCPVCRKSL 271

[Close]

BLAST of MC10g0426 vs. NCBI nr
Match: XP_022149239.1 (probable E3 ubiquitin-protein ligase RHC1A isoform X3 [Momordica charantia])

HSP 1 Score: 557 bits (1435), Expect = 1.33e-200
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0


Query: 1   MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV 60
           MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV
Sbjct: 1   MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV 60

Query: 61  ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES 120
           ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES
Sbjct: 61  ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES 120

Query: 121 SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV 180
           SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV
Sbjct: 121 SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV 180

Query: 181 PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR 240
           PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR
Sbjct: 181 PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR 240

Query: 241 RLRYADSGDSFFVIE 255
           RLRYADSGDSFFVIE
Sbjct: 241 RLRYADSGDSFFVIE 255

[Close]

BLAST of MC10g0426 vs. NCBI nr
Match: XP_022149238.1 (E3 ubiquitin-protein ligase RDUF2-like isoform X2 [Momordica charantia])

HSP 1 Score: 557 bits (1435), Expect = 1.38e-200
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0


Query: 1   MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV 60
           MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV
Sbjct: 1   MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV 60

Query: 61  ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES 120
           ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES
Sbjct: 61  ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES 120

Query: 121 SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV 180
           SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV
Sbjct: 121 SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV 180

Query: 181 PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR 240
           PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR
Sbjct: 181 PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR 240

Query: 241 RLRYADSGDSFFVIE 255
           RLRYADSGDSFFVIE
Sbjct: 241 RLRYADSGDSFFVIE 255

[Close]

BLAST of MC10g0426 vs. NCBI nr
Match: XP_022149237.1 (E3 ubiquitin-protein ligase RDUF2-like isoform X1 [Momordica charantia])

HSP 1 Score: 557 bits (1435), Expect = 2.31e-200
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0


Query: 1   MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV 60
           MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV
Sbjct: 1   MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV 60

Query: 61  ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES 120
           ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES
Sbjct: 61  ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES 120

Query: 121 SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV 180
           SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV
Sbjct: 121 SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV 180

Query: 181 PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR 240
           PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR
Sbjct: 181 PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR 240

Query: 241 RLRYADSGDSFFVIE 255
           RLRYADSGDSFFVIE
Sbjct: 241 RLRYADSGDSFFVIE 255

[Close]

BLAST of MC10g0426 vs. NCBI nr
Match: XP_022982362.1 (uncharacterized protein LOC111481213 isoform X1 [Cucurbita maxima])

HSP 1 Score: 340 bits (873), Expect = 4.27e-115
Identity = 167/257 (64.98%), Postives = 189/257 (73.54%), Query Frame = 0


Query: 1   MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV 60
           MSQN RPRIA N G+ RT T+HYYWCRICRRIIRIS GNPLEISI+CPFCSR LRHELDV
Sbjct: 1   MSQNFRPRIAFNAGIPRTRTVHYYWCRICRRIIRISLGNPLEISITCPFCSRNLRHELDV 60

Query: 61  ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES 120
            RARFH DPS FL +S        +SRR +P W FE DD  S +SWITLQFSRP    E+
Sbjct: 61  VRARFHGDPSNFLQDSGNHMT-NTNSRRMIPRWTFETDDAHSLDSWITLQFSRPS---EN 120

Query: 121 SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV 180
           S+ P +S      EAI RVKITE HL KDS C ICKEEF IGGE +ELPC+HFYHSDCIV
Sbjct: 121 SNPPANS----DTEAIRRVKITESHLQKDSSCAICKEEFEIGGEVKELPCRHFYHSDCIV 180

Query: 181 PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR 240
           PWLR+ NTCPVCR+TLQN   A+   +    VEEG GR NGWW+ + SWWPFR IG  AR
Sbjct: 181 PWLRIHNTCPVCRYTLQN---AIDGESFEESVEEGDGRENGWWNVVRSWWPFRVIGDLAR 240

Query: 241 RLRYADSGDSFFVIERK 257
           RL +AD+GD+   +E K
Sbjct: 241 RLGFADNGDNLLALETK 246

[Close]

BLAST of MC10g0426 vs. NCBI nr
Match: XP_022942764.1 (uncharacterized protein LOC111447704 isoform X1 [Cucurbita moschata] >XP_022942765.1 uncharacterized protein LOC111447704 isoform X1 [Cucurbita moschata])

HSP 1 Score: 340 bits (872), Expect = 6.05e-115
Identity = 166/257 (64.59%), Postives = 189/257 (73.54%), Query Frame = 0


Query: 1   MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV 60
           MSQN RPRIA N G+ RT T+HYYWCRICRRIIRIS GNPLEISI+CPFCSR LRHELDV
Sbjct: 1   MSQNFRPRIAFNAGIPRTRTVHYYWCRICRRIIRISLGNPLEISITCPFCSRNLRHELDV 60

Query: 61  ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES 120
            RARFH DPS FL +S        +SRR +P W FE DD  S +SWITLQFSRP     S
Sbjct: 61  VRARFHGDPSNFLQDSGNHMT-NTNSRRMIPRWTFETDDPHSLDSWITLQFSRP-----S 120

Query: 121 SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV 180
            ++ P +  D  +EAI RVKITE HL KDS C ICKEEF IGGE RELPC+HFYHSDCIV
Sbjct: 121 ENSNPAANSD--MEAIRRVKITESHLQKDSSCAICKEEFEIGGEVRELPCRHFYHSDCIV 180

Query: 181 PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR 240
           PWLR+ NTCP+CR+TLQN   A+   ++   VEEG GR NGWW+ + SWWPFR IG  AR
Sbjct: 181 PWLRIHNTCPICRYTLQN---AIDGESSEESVEEGDGRGNGWWNVVRSWWPFRVIGDLAR 240

Query: 241 RLRYADSGDSFFVIERK 257
           RL +AD GD+   +E K
Sbjct: 241 RLGFADYGDNLLALEPK 246

[Close]

BLAST of MC10g0426 vs. ExPASy TrEMBL
Match: A0A6J1D6G1 (probable E3 ubiquitin-protein ligase RHC1A isoform X3 OS=Momordica charantia OX=3673 GN=LOC111017708 PE=4 SV=1)

HSP 1 Score: 557 bits (1435), Expect = 6.42e-201
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0


Query: 1   MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV 60
           MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV
Sbjct: 1   MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV 60

Query: 61  ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES 120
           ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES
Sbjct: 61  ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES 120

Query: 121 SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV 180
           SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV
Sbjct: 121 SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV 180

Query: 181 PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR 240
           PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR
Sbjct: 181 PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR 240

Query: 241 RLRYADSGDSFFVIE 255
           RLRYADSGDSFFVIE
Sbjct: 241 RLRYADSGDSFFVIE 255

[Close]

BLAST of MC10g0426 vs. ExPASy TrEMBL
Match: A0A6J1D6A2 (E3 ubiquitin-protein ligase RDUF2-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017708 PE=4 SV=1)

HSP 1 Score: 557 bits (1435), Expect = 6.67e-201
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0


Query: 1   MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV 60
           MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV
Sbjct: 1   MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV 60

Query: 61  ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES 120
           ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES
Sbjct: 61  ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES 120

Query: 121 SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV 180
           SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV
Sbjct: 121 SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV 180

Query: 181 PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR 240
           PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR
Sbjct: 181 PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR 240

Query: 241 RLRYADSGDSFFVIE 255
           RLRYADSGDSFFVIE
Sbjct: 241 RLRYADSGDSFFVIE 255

[Close]

BLAST of MC10g0426 vs. ExPASy TrEMBL
Match: A0A6J1D7E1 (E3 ubiquitin-protein ligase RDUF2-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017708 PE=4 SV=1)

HSP 1 Score: 557 bits (1435), Expect = 1.12e-200
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0


Query: 1   MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV 60
           MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV
Sbjct: 1   MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV 60

Query: 61  ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES 120
           ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES
Sbjct: 61  ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES 120

Query: 121 SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV 180
           SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV
Sbjct: 121 SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV 180

Query: 181 PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR 240
           PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR
Sbjct: 181 PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR 240

Query: 241 RLRYADSGDSFFVIE 255
           RLRYADSGDSFFVIE
Sbjct: 241 RLRYADSGDSFFVIE 255

[Close]

BLAST of MC10g0426 vs. ExPASy TrEMBL
Match: A0A6J1IWF5 (uncharacterized protein LOC111481213 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481213 PE=4 SV=1)

HSP 1 Score: 340 bits (873), Expect = 2.07e-115
Identity = 167/257 (64.98%), Postives = 189/257 (73.54%), Query Frame = 0


Query: 1   MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV 60
           MSQN RPRIA N G+ RT T+HYYWCRICRRIIRIS GNPLEISI+CPFCSR LRHELDV
Sbjct: 1   MSQNFRPRIAFNAGIPRTRTVHYYWCRICRRIIRISLGNPLEISITCPFCSRNLRHELDV 60

Query: 61  ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES 120
            RARFH DPS FL +S        +SRR +P W FE DD  S +SWITLQFSRP    E+
Sbjct: 61  VRARFHGDPSNFLQDSGNHMT-NTNSRRMIPRWTFETDDAHSLDSWITLQFSRPS---EN 120

Query: 121 SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV 180
           S+ P +S      EAI RVKITE HL KDS C ICKEEF IGGE +ELPC+HFYHSDCIV
Sbjct: 121 SNPPANS----DTEAIRRVKITESHLQKDSSCAICKEEFEIGGEVKELPCRHFYHSDCIV 180

Query: 181 PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR 240
           PWLR+ NTCPVCR+TLQN   A+   +    VEEG GR NGWW+ + SWWPFR IG  AR
Sbjct: 181 PWLRIHNTCPVCRYTLQN---AIDGESFEESVEEGDGRENGWWNVVRSWWPFRVIGDLAR 240

Query: 241 RLRYADSGDSFFVIERK 257
           RL +AD+GD+   +E K
Sbjct: 241 RLGFADNGDNLLALETK 246

[Close]

BLAST of MC10g0426 vs. ExPASy TrEMBL
Match: A0A6J1FWW3 (uncharacterized protein LOC111447704 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447704 PE=4 SV=1)

HSP 1 Score: 340 bits (872), Expect = 2.93e-115
Identity = 166/257 (64.59%), Postives = 189/257 (73.54%), Query Frame = 0


Query: 1   MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV 60
           MSQN RPRIA N G+ RT T+HYYWCRICRRIIRIS GNPLEISI+CPFCSR LRHELDV
Sbjct: 1   MSQNFRPRIAFNAGIPRTRTVHYYWCRICRRIIRISLGNPLEISITCPFCSRNLRHELDV 60

Query: 61  ARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPES 120
            RARFH DPS FL +S        +SRR +P W FE DD  S +SWITLQFSRP     S
Sbjct: 61  VRARFHGDPSNFLQDSGNHMT-NTNSRRMIPRWTFETDDPHSLDSWITLQFSRP-----S 120

Query: 121 SDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIV 180
            ++ P +  D  +EAI RVKITE HL KDS C ICKEEF IGGE RELPC+HFYHSDCIV
Sbjct: 121 ENSNPAANSD--MEAIRRVKITESHLQKDSSCAICKEEFEIGGEVRELPCRHFYHSDCIV 180

Query: 181 PWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIGGWAR 240
           PWLR+ NTCP+CR+TLQN   A+   ++   VEEG GR NGWW+ + SWWPFR IG  AR
Sbjct: 181 PWLRIHNTCPICRYTLQN---AIDGESSEESVEEGDGRGNGWWNVVRSWWPFRVIGDLAR 240

Query: 241 RLRYADSGDSFFVIERK 257
           RL +AD GD+   +E K
Sbjct: 241 RLGFADYGDNLLALEPK 246

[Close]

BLAST of MC10g0426 vs. TAIR 10
Match: AT1G68180.1 (RING/U-box superfamily protein )

HSP 1 Score: 142.1 bits (357), Expect = 6.2e-34
Identity = 92/267 (34.46%), Postives = 122/267 (45.69%), Query Frame = 0


Query: 1   MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRI-SFG--NPLEISISCPFCSR--LLR 60
           M+   RPR+ +N G  RT T HY++CR C R IR+ SFG   P+     CPFCSR   L 
Sbjct: 1   MTSAYRPRVIVN-GTRRTRTFHYFYCRHCSRTIRLRSFGLYGPI-----CPFCSREINLH 60

Query: 61  HELDVARARFHADPSGFLPNSSRLPDPRPDSRRNLPLWGFEIDDGPSPESWITLQ-FSRP 120
            ELD+ R                          + P W  + D       WITL   + P
Sbjct: 61  DELDIMRP-------------------------SRPYWDSDTD-------WITLHLINEP 120

Query: 121 RA-------------------LPESSDAPPDSRQDCGIEAIPRVKITEYHLGKDSICPIC 180
           R+                   +P     PP + Q   IEA+  V IT+  L K+ +C IC
Sbjct: 121 RSNRFNHDDLVYNTDEEFADVMPSVQIGPPPASQS-AIEAVRTVIITDEDLVKEKVCAIC 180

Query: 181 KEEFRIGGEARELPCKHFYHSDCIVPWLRLRNTCPVCRHTLQNIPVAVAVAAAGTFVEEG 240
           KEEF +G E +EL C H YHS CIV WL + NTCP+CR  + N+ V+ +    G      
Sbjct: 181 KEEFEVGEEGKELKCLHLYHSSCIVSWLNIHNTCPICRFEV-NLGVSESNVDEGGSYNID 227

Query: 241 GGRWNGWWSSICSWWPFRSIGGWARRL 243
             R N + + +CS WP R +  W   L
Sbjct: 241 NDRSNRFRTRVCSLWPLRMMIDWVHNL 227

[Close]

BLAST of MC10g0426 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 119.4 bits (298), Expect = 4.3e-27
Identity = 96/311 (30.87%), Postives = 131/311 (42.12%), Query Frame = 0


Query: 1   MSQNLRPRIAINGGVHRTTTLHYYWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDV 60
           +SQ +    +   G  RT  L  YWC  C R++RI+  NP E  I+CP C R    E++ 
Sbjct: 3   LSQPITRTDSAPNGAFRTFGL--YWCYHCDRMVRIASSNPSE--IACPRCLRQFVVEIET 62

Query: 61  AR----------------------------------ARFHADPSGFLPNSSR---LPD-- 120
            +                                   RF ADP  FL   SR    P+  
Sbjct: 63  RQRPRFTFNHATPPFDASPEARLLEALSLMFEPATIGRFGADP--FLRARSRNILEPESR 122

Query: 121 PRPDSRRNLPLWGFEIDDGPSP-ESWITLQFSRPRA-----LPESSDAPP---------- 180
           PRP  RR   L        P P  +++ L+ + P +     +   + APP          
Sbjct: 123 PRPQHRRRHSLDNVNNGGLPLPRRTYVILRPNNPTSPLGNIIAPPNQAPPRHVNSHDYFT 182

Query: 181 --------------DSR------QDCGIEAIPRVKITEYHLGKD-SICPICKEEFRIGGE 235
                         D R       +  I ++P VKIT  HL  D S C +C EEF +GG+
Sbjct: 183 GASSLEQLIEQLTQDDRPGPPPASEPTINSLPSVKITPQHLTNDMSQCTVCMEEFIVGGD 242

[Close]

BLAST of MC10g0426 vs. TAIR 10
Match: AT3G10815.1 (RING/U-box superfamily protein )

HSP 1 Score: 108.2 bits (269), Expect = 9.9e-24
Identity = 71/213 (33.33%), Postives = 98/213 (46.01%), Query Frame = 0


Query: 24  YWCRICRRIIRISFGNPLEISISCPFCSRLLRHELDVARARFHADPSGFLPNSSRLPDPR 83
           +WC  CRR I +      E   +C +C       L         D  G     +R    R
Sbjct: 10  HWCHTCRRGIHLQGEGRRE--GACIYCGNTFLERLYENVELSPFDLFGLAYEEAR---NR 69

Query: 84  PDSRRNLPLWGFEIDDGPSPESWITLQFSRPRALPESSDAPPDSRQDCGIEAIPRVKITE 143
            ++RR++      I +  S E      F+R  AL +    PP S     I ++ ++KI +
Sbjct: 70  RNNRRSV------IGEQLSFEE----LFNRLPALQDRRGPPPASL--AAINSLQKIKIRQ 129

Query: 144 YHLGKDSICPICKEEFRIGGEARELPCKHFYHSDCIVPWLRLRNTCPVCRHTLQNIPVAV 203
            HLG D  CP+C+++F IG +AR++PCKH YHS+CI+PWL  RNTCPVCR  L       
Sbjct: 130 KHLGLDPYCPVCQDQFEIGSDARKMPCKHIYHSECILPWLVQRNTCPVCRKELPQ----- 187

Query: 204 AVAAAGTFVEEGGGRWNGWWSSICSWWPFRSIG 237
                    +    R N  W      WPFRS G
Sbjct: 190 ---------DRNNSRKNPLW----HLWPFRSSG 187

[Close]

BLAST of MC10g0426 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 102.4 bits (254), Expect = 5.4e-22
Identity = 53/125 (42.40%), Postives = 68/125 (54.40%), Query Frame = 0


Query: 124 PPDSRQDCGIEAIPRVKITEYHL-GKDSICPICKEEFRIGGEARELPCKHFYHSDCIVPW 183
           PP  R    I+A+P +KI + HL   DS CP+CK+EF +G EA+++PC H YHSDCIVPW
Sbjct: 163 PPAPRS--AIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPW 222

Query: 184 LRLRNTCPVCRHTL---------QNIPVAVAVAAAGTFVEEGGGRWNG--WWSSICSWWP 237
           L   N+CPVCR  L         QN         + +       R NG    +   S+WP
Sbjct: 223 LVQHNSCPVCRQELPSASGPSSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWP 282

[Close]

BLAST of MC10g0426 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 102.4 bits (254), Expect = 5.4e-22
Identity = 53/125 (42.40%), Postives = 68/125 (54.40%), Query Frame = 0


Query: 124 PPDSRQDCGIEAIPRVKITEYHL-GKDSICPICKEEFRIGGEARELPCKHFYHSDCIVPW 183
           PP  R    I+A+P +KI + HL   DS CP+CK+EF +G EA+++PC H YHSDCIVPW
Sbjct: 163 PPAPRS--AIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPW 222

Query: 184 LRLRNTCPVCRHTL---------QNIPVAVAVAAAGTFVEEGGGRWNG--WWSSICSWWP 237
           L   N+CPVCR  L         QN         + +       R NG    +   S+WP
Sbjct: 223 LVQHNSCPVCRQELPSASGPSSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWP 282

The following BLAST results are available for this feature:

BLAST of MC10g0426 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22197	7.6e-21	42.40	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q940T5	4.9e-20	51.25	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]
Q94AK4	1.1e-19	43.75	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]
Q9SNB6	2.5e-19	49.38	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
Q6DIP3	9.3e-19	46.15	E3 ubiquitin-protein ligase RNF126 OS=Xenopus tropicalis OX=8364 GN=rnf126 PE=2 ...	[more]

BLAST of MC10g0426 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149239.1	1.33e-200	100.00	probable E3 ubiquitin-protein ligase RHC1A isoform X3 [Momordica charantia]	[more]
XP_022149238.1	1.38e-200	100.00	E3 ubiquitin-protein ligase RDUF2-like isoform X2 [Momordica charantia]	[more]
XP_022149237.1	2.31e-200	100.00	E3 ubiquitin-protein ligase RDUF2-like isoform X1 [Momordica charantia]	[more]
XP_022982362.1	4.27e-115	64.98	uncharacterized protein LOC111481213 isoform X1 [Cucurbita maxima]	[more]
XP_022942764.1	6.05e-115	64.59	uncharacterized protein LOC111447704 isoform X1 [Cucurbita moschata] >XP_0229427...	[more]

BLAST of MC10g0426 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D6G1	6.42e-201	100.00	probable E3 ubiquitin-protein ligase RHC1A isoform X3 OS=Momordica charantia OX=...	[more]
A0A6J1D6A2	6.67e-201	100.00	E3 ubiquitin-protein ligase RDUF2-like isoform X2 OS=Momordica charantia OX=3673...	[more]
A0A6J1D7E1	1.12e-200	100.00	E3 ubiquitin-protein ligase RDUF2-like isoform X1 OS=Momordica charantia OX=3673...	[more]
A0A6J1IWF5	2.07e-115	64.98	uncharacterized protein LOC111481213 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1FWW3	2.93e-115	64.59	uncharacterized protein LOC111447704 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of MC10g0426 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G68180.1	6.2e-34	34.46	RING/U-box superfamily protein	[more]
AT1G60360.1	4.3e-27	30.87	RING/U-box superfamily protein	[more]
AT3G10815.1	9.9e-24	33.33	RING/U-box superfamily protein	[more]
AT2G40830.2	5.4e-22	42.40	RING-H2 finger C1A	[more]
AT2G40830.1	5.4e-22	42.40	RING-H2 finger C1A	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 152..192 e-value: 3.7E-8 score: 43.1
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 152..193 e-value: 9.5E-12 score: 45.1
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 152..193 score: 12.267982
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 118..199 e-value: 1.7E-19 score: 71.3
None	No IPR available	PANTHER	PTHR15710:SF116	ZINC FINGER, C3HC4 TYPE (RING FINGER) PROTEIN	coord: 22..238
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 22..238
None	No IPR available	CDD	cd16667	RING-H2_RNF126_like	coord: 152..193 e-value: 8.84108E-23 score: 85.8324
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 133..199

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g0426.1	MC10g0426.1	mRNA