MC10g0327 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g0327
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionXyloglucan endotransglucosylase/hydrolase
LocationMC10: 2644164 .. 2646274 (-)
RNA-Seq ExpressionMC10g0327
SyntenyMC10g0327
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAGGAGATCAAGAAAAAGCCATGTATGAAAGTGTTAAAACTATCATCTTTTTTGGAGTTTTCATTGCAAACTTGCTTCAATTTGCAACATCTTCCGTCATTTCAACTGGGGACTTCAACAAGGATTTCTTCATCACTTGGTCTCCTTCCCACGTTAACACTTCGGCGGATGGCCGAGCAAGAACCTTGAAGCTCGATAAGGATTCAGGTACGGGTGTTCAAGAAACTCGATGATCACTTGAAAATTTTCGTTCAAATTAAACCAATAATCTAAATTGTTCGAGTCGATGAGTTAGTTAGGTTATTGGTTTATACACATGTTCGTATGTTTATGAGATTAACTATTCCTTTGCCTCCAAGGGACGTACAGCCCGATTGACAAAGGGCATGAGACCTCTTCGTCATCCTGGCTTAGAGGTCTCAAGTTCGAATCTTCTAGTGAGTTTAATACCAAAAATCATTATATGTCTCTCGAATCCGAATCTTGAGTCGGACATAGGTGCCTCTAGATATAAGGGAGCAAAATTCCAACTCACAGTTATAATTCACTACTCCTTTATTTGTTCACTGTAATATATCATTTTTAGATAAATTATTCAAATGTCAGTATTTTTGTCATCCATAGGAGCTGCTTTTTCTTCAAATGAGATGTTCTTATTTGGTCAAATGGACATGAAAATCAAGCTAGTTCCAGGCCATTCTGCAGGCACAGTTGTGGCCTATTATGTAAGTGGTCGTTTTCTCCTTTCATGTTTTTTAATATATGATTCTTTTTTCTTATATTATATATATCAATGCTAATATTACTTGAACTATGTGTAGCTAACGTCGGATCAACCGAACCGCGACGAAATCGACTTCGAGTTCCTCGGGAATGTGGACGGGGAGCCATATATTCTTCAAACAAATGTTTTTGCTGATGGTTTTGATGACAGGGAAGAAAGGATAAAGTTGTGGTTTGATCCAACAAAAGACTTCCATACATATTCAATTTTGTGGAACCGTTATCAGATTGTGTAAGAAAATCACTTTAATTTTCTTGCTTCTTTTTGGATTCTGCATTTTAATTCCTCTTTTTAAGCCTACAAAAACCCATAAAAAGTTGACAAAAGTAGCATAACTTTGAACATGGTGGATGAGATGAATGTGAAGGAATTAGCATTAAGGTTGATAGTTAAGAAAAGATTTTACATTTTAATTTAATTACTACATTGATTAAACAACTTAATTTGAATAGGGGAGACCCCTATTGTTATTGCTAGTGTCTCTCTCAAATTAATGTCACAACTTTTTACTATTGAATTTGATTTTTCTAGTTAAAAAGTTTTTGAATTAGACACAATTGAATTAATTTAAACACGTTCACCAATCATCAAACTCTTAATAATTTTTTATCAACCTAAACATAGGTAAATAGATAAAACCATCATAGGTTGACTTATTGGTCAAAAGAAGCTAGGGTTAATTGAGACGGGAAGAATTCAAACTCACGATAGCCACTTACCTTAAAGATTAGTAATTCTTATGTGTTTCCCAACACTAAATGTTGCAAGATCAAGTGATTGTTTTATAAGATTAGTCGGAGAAGTTACTAATATCTAAACGCTTTAGTCACTTTACATCTCTAGAAAACTCAACAAATCAAATCTTCTTCACTATAAAACCATTCCAATTTTCTTGTTTGTTTTTTGTTTTGTTGTTGTTGTAGGTTCATGGTGGATTGGGTGCCAATCAGAGTGTACAGAAACCATGCAGACAAAGGGGTGGGATATCCTAGGTGGCAGCCAATGAGCTTGAAAGTGAGCCTATGGGATGGTGACAGCTGGGCAACAAGGGGTGGAAAAGACAAAATTGATTGGTCAAAAGGGCCATTTGTAGCTTCTTTTGGGGATTATAAGGTGGATGCCTGTGTTTGGAGGGGAAATGCAAGGTTATGCAGAGGAGATGATCATAGTTATTGGTGGAATTTGCCCAAATACAACACCCTTTCACCTCCACAAAGAAGGCTCTTCAAATGGGTCAGAAACTACCATTTGATTTATGATTATTGCCAAGACAACTTGAGGTTCCAAAACCACCTCCCAAAGGAATGTTCTCTTCCCAAGTAC

mRNA sequence

ATGAGAGGAGATCAAGAAAAAGCCATGTATGAAAGTGTTAAAACTATCATCTTTTTTGGAGTTTTCATTGCAAACTTGCTTCAATTTGCAACATCTTCCGTCATTTCAACTGGGGACTTCAACAAGGATTTCTTCATCACTTGGTCTCCTTCCCACGTTAACACTTCGGCGGATGGCCGAGCAAGAACCTTGAAGCTCGATAAGGATTCAGGAGCTGCTTTTTCTTCAAATGAGATGTTCTTATTTGGTCAAATGGACATGAAAATCAAGCTAGTTCCAGGCCATTCTGCAGGCACAGTTGTGGCCTATTATCTAACGTCGGATCAACCGAACCGCGACGAAATCGACTTCGAGTTCCTCGGGAATGTGGACGGGGAGCCATATATTCTTCAAACAAATGTTTTTGCTGATGGTTTTGATGACAGGGAAGAAAGGATAAAGTTGTGGTTTGATCCAACAAAAGACTTCCATACATATTCAATTTTGTGGAACCGTTATCAGATTGTGTTCATGGTGGATTGGGTGCCAATCAGAGTGTACAGAAACCATGCAGACAAAGGGGTGGGATATCCTAGGTGGCAGCCAATGAGCTTGAAAGTGAGCCTATGGGATGGTGACAGCTGGGCAACAAGGGGTGGAAAAGACAAAATTGATTGGTCAAAAGGGCCATTTGTAGCTTCTTTTGGGGATTATAAGGTGGATGCCTGTGTTTGGAGGGGAAATGCAAGGTTATGCAGAGGAGATGATCATAGTTATTGGTGGAATTTGCCCAAATACAACACCCTTTCACCTCCACAAAGAAGGCTCTTCAAATGGGTCAGAAACTACCATTTGATTTATGATTATTGCCAAGACAACTTGAGGTTCCAAAACCACCTCCCAAAGGAATGTTCTCTTCCCAAGTAC

Coding sequence (CDS)

ATGAGAGGAGATCAAGAAAAAGCCATGTATGAAAGTGTTAAAACTATCATCTTTTTTGGAGTTTTCATTGCAAACTTGCTTCAATTTGCAACATCTTCCGTCATTTCAACTGGGGACTTCAACAAGGATTTCTTCATCACTTGGTCTCCTTCCCACGTTAACACTTCGGCGGATGGCCGAGCAAGAACCTTGAAGCTCGATAAGGATTCAGGAGCTGCTTTTTCTTCAAATGAGATGTTCTTATTTGGTCAAATGGACATGAAAATCAAGCTAGTTCCAGGCCATTCTGCAGGCACAGTTGTGGCCTATTATCTAACGTCGGATCAACCGAACCGCGACGAAATCGACTTCGAGTTCCTCGGGAATGTGGACGGGGAGCCATATATTCTTCAAACAAATGTTTTTGCTGATGGTTTTGATGACAGGGAAGAAAGGATAAAGTTGTGGTTTGATCCAACAAAAGACTTCCATACATATTCAATTTTGTGGAACCGTTATCAGATTGTGTTCATGGTGGATTGGGTGCCAATCAGAGTGTACAGAAACCATGCAGACAAAGGGGTGGGATATCCTAGGTGGCAGCCAATGAGCTTGAAAGTGAGCCTATGGGATGGTGACAGCTGGGCAACAAGGGGTGGAAAAGACAAAATTGATTGGTCAAAAGGGCCATTTGTAGCTTCTTTTGGGGATTATAAGGTGGATGCCTGTGTTTGGAGGGGAAATGCAAGGTTATGCAGAGGAGATGATCATAGTTATTGGTGGAATTTGCCCAAATACAACACCCTTTCACCTCCACAAAGAAGGCTCTTCAAATGGGTCAGAAACTACCATTTGATTTATGATTATTGCCAAGACAACTTGAGGTTCCAAAACCACCTCCCAAAGGAATGTTCTCTTCCCAAGTAC

Protein sequence

MRGDQEKAMYESVKTIIFFGVFIANLLQFATSSVISTGDFNKDFFITWSPSHVNTSADGRARTLKLDKDSGAAFSSNEMFLFGQMDMKIKLVPGHSAGTVVAYYLTSDQPNRDEIDFEFLGNVDGEPYILQTNVFADGFDDREERIKLWFDPTKDFHTYSILWNRYQIVFMVDWVPIRVYRNHADKGVGYPRWQPMSLKVSLWDGDSWATRGGKDKIDWSKGPFVASFGDYKVDACVWRGNARLCRGDDHSYWWNLPKYNTLSPPQRRLFKWVRNYHLIYDYCQDNLRFQNHLPKECSLPKY
Homology
BLAST of MC10g0327 vs. ExPASy Swiss-Prot
Match: Q9ZVK1 (Probable xyloglucan endotransglucosylase/hydrolase protein 10 OS=Arabidopsis thaliana OX=3702 GN=XTH10 PE=2 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 6.4e-128
Identity = 203/287 (70.73%), Postives = 244/287 (85.02%), Query Frame = 0

Query: 16  IIFFGVFIANLLQFATSSVISTGDFNKDFFITWSPSHVNTSADGRARTLKLDKDSGAAFS 75
           ++ F +  + LL  + +SV+S+GDFNKDFF+TWSP+HVNTS DGR+RTLKLD++SGA+FS
Sbjct: 13  LVGFSIISSLLLWVSQASVVSSGDFNKDFFVTWSPTHVNTSNDGRSRTLKLDQESGASFS 72

Query: 76  SNEMFLFGQMDMKIKLVPGHSAGTVVAYYLTSDQPNRDEIDFEFLGNVDGEPYILQTNVF 135
           S + FLFGQ+DMKIKL+ G S GTVVAYY++SDQPNRDEIDFEFLGNV+G+PYILQTNV+
Sbjct: 73  SIQTFLFGQIDMKIKLIRGSSQGTVVAYYMSSDQPNRDEIDFEFLGNVNGQPYILQTNVY 132

Query: 136 ADGFDDREERIKLWFDPTKDFHTYSILWNRYQIVFMVDWVPIRVYRNHADKGVGYPRWQP 195
           A+G D+REERI LWFDP KDFHTYSILWN +QIVFMVD +PIR+YRNH +KGV YPR QP
Sbjct: 133 AEGLDNREERIHLWFDPAKDFHTYSILWNIHQIVFMVDQIPIRLYRNHGEKGVAYPRLQP 192

Query: 196 MSLKVSLWDGDSWATRGGKDKIDWSKGPFVASFGDYKVDACVWRGNARLCRGDDHSYWWN 255
           MS++ SLW+G+SWATRGG DKIDWSKGPFVASFGDYK+DAC+W GN   C G+    WWN
Sbjct: 193 MSVQASLWNGESWATRGGHDKIDWSKGPFVASFGDYKIDACIWIGNTSFCNGESTENWWN 252

Query: 256 LPKYNTLSPPQRRLFKWVRNYHLIYDYCQDNLRFQNHLPKECSLPKY 303
             ++++L+  Q+R FKWVR YHLIYDYCQD  RF N LPKECSLPKY
Sbjct: 253 KNEFSSLTRVQKRWFKWVRKYHLIYDYCQDYGRFNNKLPKECSLPKY 299

BLAST of MC10g0327 vs. ExPASy Swiss-Prot
Match: Q8LER3 (Probable xyloglucan endotransglucosylase/hydrolase protein 7 OS=Arabidopsis thaliana OX=3702 GN=XTH7 PE=2 SV=2)

HSP 1 Score: 288.1 bits (736), Expect = 1.1e-76
Identity = 135/260 (51.92%), Postives = 177/260 (68.08%), Query Frame = 0

Query: 40  FNKDFFITWSPSHVNTSADGRARTLKLDKDSGAAFSSNEMFLFGQMDMKIKLVPGHSAGT 99
           F  DF I WS +H+     GRA  LKLD  SG  F+S + +LFG++ MKIKL+PG SAGT
Sbjct: 34  FEDDFRIAWSDTHITQIDGGRAIQLKLDPSSGCGFASKKQYLFGRVSMKIKLIPGDSAGT 93

Query: 100 VVAYYLTSDQPN-RDEIDFEFLGNVDGEPYILQTNVFADGFDDREERIKLWFDPTKDFHT 159
           V A+Y+ SD  + RDE+DFEFLGN  G+PY +QTNVFA G  DRE+R+ LWFDP++DFH 
Sbjct: 94  VTAFYMNSDTDSVRDELDFEFLGNRSGQPYTVQTNVFAHGKGDREQRVNLWFDPSRDFHE 153

Query: 160 YSILWNRYQIVFMVDWVPIRVYRNHADKGVGYPRWQPMSLKVSLWDGDSWATRGGKDKID 219
           Y+I WN  +IVF VD VPIRVY+N+  + V YPR+QPM +  +LW+ D WATRGG +KI+
Sbjct: 154 YAISWNHLRIVFYVDNVPIRVYKNNEARKVPYPRFQPMGVYSTLWEADDWATRGGIEKIN 213

Query: 220 WSKGPFVASFGDYKVDACVWRGNARLCRGDDHSYWWNLPKYNTLSPPQRRLFKWVRNYHL 279
           WS+ PF A + D+ ++ C   G A  C  +  + WW    Y+ LSP + R ++WVR  H+
Sbjct: 214 WSRAPFYAYYKDFDIEGCPVPGPAD-CPANSKN-WWEGSAYHQLSPVEARSYRWVRVNHM 273

Query: 280 IYDYCQDNLRFQNHLPKECS 299
           +YDYC D  RF    P ECS
Sbjct: 274 VYDYCTDKSRFPVP-PPECS 290

BLAST of MC10g0327 vs. ExPASy Swiss-Prot
Match: Q41542 (Probable xyloglucan endotransglucosylase/hydrolase OS=Triticum aestivum OX=4565 GN=XTH PE=2 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.3e-75
Identity = 134/258 (51.94%), Postives = 166/258 (64.34%), Query Frame = 0

Query: 40  FNKDFFITWSPSHVNTSADGRARTLKLDKDSGAAFSSNEMFLFGQMDMKIKLVPGHSAGT 99
           F+K++  TW+  H++    GR   L LDK +G  F +   +LFG   M IKLV G SAGT
Sbjct: 32  FDKNYVPTWAQDHIHYVNGGREVQLSLDKTTGTGFQTRGSYLFGHFSMHIKLVGGDSAGT 91

Query: 100 VVAYYLTSDQPNRDEIDFEFLGNVDGEPYILQTNVFADGFDDREERIKLWFDPTKDFHTY 159
           V A+YL+S     DEIDFEFLGN  G+PYILQTNVF+ G  DRE+RI LWFDPTKD+H+Y
Sbjct: 92  VTAFYLSSQNSEHDEIDFEFLGNRTGQPYILQTNVFSGGKGDREQRIYLWFDPTKDYHSY 151

Query: 160 SILWNRYQIVFMVDWVPIRVYRNHADKGVGYPRWQPMSLKVSLWDGDSWATRGGKDKIDW 219
           S+LWN Y I F VD  PIRV++N  D GV YP  QPM L  SLW+ D WATRGG++K DW
Sbjct: 152 SVLWNLYMIAFFVDDTPIRVFKNSKDLGVRYPFDQPMKLYSSLWNADDWATRGGREKTDW 211

Query: 220 SKGPFVASFGDYKVDACVWRGNARLCRGDDHSYWWNLPKYNTLSPPQRRLFKWVRNYHLI 279
           SK PFVAS+  + VD C     A+ C     + WW+ P++  L   Q R   WVR  H I
Sbjct: 212 SKAPFVASYRGFHVDGCEASAEAKFC-ATQGARWWDQPEFQDLDAAQYRRLAWVRKEHTI 271

Query: 280 YDYCQDNLRFQNHLPKEC 298
           Y+YC D+ R+    P EC
Sbjct: 272 YNYCTDHDRYAAMAP-EC 287

BLAST of MC10g0327 vs. ExPASy Swiss-Prot
Match: Q8LF99 (Probable xyloglucan endotransglucosylase/hydrolase protein 6 OS=Arabidopsis thaliana OX=3702 GN=XTH6 PE=2 SV=2)

HSP 1 Score: 279.6 bits (714), Expect = 4.1e-74
Identity = 129/259 (49.81%), Postives = 171/259 (66.02%), Query Frame = 0

Query: 40  FNKDFFITWSPSHVNTSADGRARTLKLDKDSGAAFSSNEMFLFGQMDMKIKLVPGHSAGT 99
           F +DF   WS SH+    DG+A  L LD+ +G  F+S   +LFG++ MKIKL+PG SAGT
Sbjct: 35  FVEDFKAAWSESHIRQMEDGKAIQLVLDQSTGCGFASKRKYLFGRVSMKIKLIPGDSAGT 94

Query: 100 VVAYYLTSDQPN-RDEIDFEFLGNVDGEPYILQTNVFADGFDDREERIKLWFDPTKDFHT 159
           V A+Y+ SD    RDE+DFEFLGN  G+PY +QTN+FA G  DRE+R+ LWFDP+ D+HT
Sbjct: 95  VTAFYMNSDTATVRDELDFEFLGNRSGQPYSVQTNIFAHGKGDREQRVNLWFDPSMDYHT 154

Query: 160 YSILWNRYQIVFMVDWVPIRVYRNHADKGVGYPRWQPMSLKVSLWDGDSWATRGGKDKID 219
           Y+ILW+   IVF VD VPIR Y+N+  K + YP  QPM +  +LW+ D WATRGG +KID
Sbjct: 155 YTILWSHKHIVFYVDDVPIREYKNNEAKNIAYPTSQPMGVYSTLWEADDWATRGGLEKID 214

Query: 220 WSKGPFVASFGDYKVDACVWRGNARLCRGDDHSYWWNLPKYNTLSPPQRRLFKWVRNYHL 279
           WSK PF A + D+ ++ C   G    C  + H+ WW    Y +L+  + R ++WVR  H+
Sbjct: 215 WSKAPFYAYYKDFDIEGCPVPG-PTFCPSNPHN-WWEGYAYQSLNAVEARRYRWVRVNHM 274

Query: 280 IYDYCQDNLRFQNHLPKEC 298
           +YDYC D  RF    P EC
Sbjct: 275 VYDYCTDRSRFPVP-PPEC 290

BLAST of MC10g0327 vs. ExPASy Swiss-Prot
Match: Q9FKL9 (Probable xyloglucan endotransglucosylase/hydrolase protein 12 OS=Arabidopsis thaliana OX=3702 GN=XTH12 PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 9.1e-74
Identity = 133/270 (49.26%), Postives = 173/270 (64.07%), Query Frame = 0

Query: 35  ISTGDFNKDFFITWSPSHVNTSADGRARTLKLDKDSGAAFSSNEMFLFGQMDMKIKLVPG 94
           ++TG F   F ITW     N    G+  T  LDK SG+ F S + +LFG++DMKIKLVPG
Sbjct: 22  VATGSFYDSFDITWGAGRANIFESGQLLTCTLDKTSGSGFQSKKEYLFGKIDMKIKLVPG 81

Query: 95  HSAGTVVAYYLTSDQPNRDEIDFEFLGNVDGEPYILQTNVFADGFDDREERIKLWFDPTK 154
           +SAGTV AYYL+S     DEIDFEFLGNV G+PY++ TNVF  G  +RE +  LWFDPT 
Sbjct: 82  NSAGTVTAYYLSSKGETWDEIDFEFLGNVTGQPYVIHTNVFTGGKGNREMQFYLWFDPTA 141

Query: 155 DFHTYSILWNRYQIVFMVDWVPIRVYRNHADKGVGYPRWQPMSLKVSLWDGDSWATRGGK 214
           DFHTY++LWN   I+F+VD +PIRV++N+   GV YP+ QPM +  SLW+ D WAT+GGK
Sbjct: 142 DFHTYTVLWNPLNIIFLVDGIPIRVFKNNEANGVAYPKSQPMKIYSSLWEADDWATQGGK 201

Query: 215 DKIDWSKGPFVASFGDYK-VDAC----VWRGNARLCRGDDHSYWWNLPKYNTLSPPQRRL 274
            K DW+  PF AS+  +  VD C    +W  N   C  + +S+ W      TL+  Q   
Sbjct: 202 VKTDWTNAPFSASYRSFNDVDCCSRTSIW--NWVTCNANSNSWMW-----TTLNSNQLGQ 261

Query: 275 FKWVRNYHLIYDYCQDNLRFQNHLPKECSL 300
            KWV+  ++IY+YC D  RF   LP EC+L
Sbjct: 262 LKWVQKDYMIYNYCTDFKRFPQGLPTECNL 284

BLAST of MC10g0327 vs. NCBI nr
Match: XP_022149246.1 (probable xyloglucan endotransglucosylase/hydrolase protein 10 [Momordica charantia])

HSP 1 Score: 645 bits (1665), Expect = 2.58e-234
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0

Query: 1   MRGDQEKAMYESVKTIIFFGVFIANLLQFATSSVISTGDFNKDFFITWSPSHVNTSADGR 60
           MRGDQEKAMYESVKTIIFFGVFIANLLQFATSSVISTGDFNKDFFITWSPSHVNTSADGR
Sbjct: 1   MRGDQEKAMYESVKTIIFFGVFIANLLQFATSSVISTGDFNKDFFITWSPSHVNTSADGR 60

Query: 61  ARTLKLDKDSGAAFSSNEMFLFGQMDMKIKLVPGHSAGTVVAYYLTSDQPNRDEIDFEFL 120
           ARTLKLDKDSGAAFSSNEMFLFGQMDMKIKLVPGHSAGTVVAYYLTSDQPNRDEIDFEFL
Sbjct: 61  ARTLKLDKDSGAAFSSNEMFLFGQMDMKIKLVPGHSAGTVVAYYLTSDQPNRDEIDFEFL 120

Query: 121 GNVDGEPYILQTNVFADGFDDREERIKLWFDPTKDFHTYSILWNRYQIVFMVDWVPIRVY 180
           GNVDGEPYILQTNVFADGFDDREERIKLWFDPTKDFHTYSILWNRYQIVFMVDWVPIRVY
Sbjct: 121 GNVDGEPYILQTNVFADGFDDREERIKLWFDPTKDFHTYSILWNRYQIVFMVDWVPIRVY 180

Query: 181 RNHADKGVGYPRWQPMSLKVSLWDGDSWATRGGKDKIDWSKGPFVASFGDYKVDACVWRG 240
           RNHADKGVGYPRWQPMSLKVSLWDGDSWATRGGKDKIDWSKGPFVASFGDYKVDACVWRG
Sbjct: 181 RNHADKGVGYPRWQPMSLKVSLWDGDSWATRGGKDKIDWSKGPFVASFGDYKVDACVWRG 240

Query: 241 NARLCRGDDHSYWWNLPKYNTLSPPQRRLFKWVRNYHLIYDYCQDNLRFQNHLPKECSLP 300
           NARLCRGDDHSYWWNLPKYNTLSPPQRRLFKWVRNYHLIYDYCQDNLRFQNHLPKECSLP
Sbjct: 241 NARLCRGDDHSYWWNLPKYNTLSPPQRRLFKWVRNYHLIYDYCQDNLRFQNHLPKECSLP 300

Query: 301 KY 302
           KY
Sbjct: 301 KY 302

BLAST of MC10g0327 vs. NCBI nr
Match: XP_008454432.1 (PREDICTED: probable xyloglucan endotransglucosylase/hydrolase protein 10 [Cucumis melo])

HSP 1 Score: 566 bits (1458), Expect = 1.04e-202
Identity = 266/305 (87.21%), Postives = 281/305 (92.13%), Query Frame = 0

Query: 1   MRGDQEKA--MYE-SVKTIIFFGVFIANLLQFATSSVISTGDFNKDFFITWSPSHVNTSA 60
           MRG   KA  MYE SVKTIIFFGVFI+N LQF+ +S ISTGD+NKDFFITWSPSHVNTS 
Sbjct: 1   MRGGNYKAIIMYECSVKTIIFFGVFISNFLQFSFASPISTGDYNKDFFITWSPSHVNTSL 60

Query: 61  DGRARTLKLDKDSGAAFSSNEMFLFGQMDMKIKLVPGHSAGTVVAYYLTSDQPNRDEIDF 120
           DGR+R LKLD DSGA F+SNEMFLFGQMDMKIKLVPG+SAGTVVAYYLTSDQPNRDEIDF
Sbjct: 61  DGRSRNLKLDNDSGAGFASNEMFLFGQMDMKIKLVPGNSAGTVVAYYLTSDQPNRDEIDF 120

Query: 121 EFLGNVDGEPYILQTNVFADGFDDREERIKLWFDPTKDFHTYSILWNRYQIVFMVDWVPI 180
           EFLGNV+G+P ILQTN+FADGFDDREERIKLWFDPTKDFHTYSILWN YQIVFMVDWVPI
Sbjct: 121 EFLGNVEGQPIILQTNIFADGFDDREERIKLWFDPTKDFHTYSILWNIYQIVFMVDWVPI 180

Query: 181 RVYRNHADKGVGYPRWQPMSLKVSLWDGDSWATRGGKDKIDWSKGPFVASFGDYKVDACV 240
           RVYRNH DKGVGYPRWQPMSLKVSLW+G+SWATRGGKDKIDWSKGPFVASFGDYKVDAC+
Sbjct: 181 RVYRNHGDKGVGYPRWQPMSLKVSLWNGESWATRGGKDKIDWSKGPFVASFGDYKVDACI 240

Query: 241 WRGNARLCRGDDHSYWWNLPKYNTLSPPQRRLFKWVRNYHLIYDYCQDNLRFQNHLPKEC 300
           WRGNAR CRG+  S WW+LPKYNTLSP QRRLFKWVR YHLIYDYCQDNLRF N LPKEC
Sbjct: 241 WRGNARFCRGESSSNWWHLPKYNTLSPSQRRLFKWVRKYHLIYDYCQDNLRFHNQLPKEC 300

Query: 301 SLPKY 302
           SLPKY
Sbjct: 301 SLPKY 305

BLAST of MC10g0327 vs. NCBI nr
Match: XP_038905402.1 (probable xyloglucan endotransglucosylase/hydrolase protein 10 [Benincasa hispida])

HSP 1 Score: 563 bits (1450), Expect = 1.66e-201
Identity = 263/303 (86.80%), Postives = 281/303 (92.74%), Query Frame = 0

Query: 1   MRGDQEKAMYESV-KTIIFFGVFIANLLQFATSSVISTGDFNKDFFITWSPSHVNTSADG 60
           MRG+  K++ E V KTI+FFGVFI NLLQF+++SVISTG++NKDFFITWSPSHVNTS DG
Sbjct: 3   MRGEN-KSINECVFKTIVFFGVFILNLLQFSSASVISTGNYNKDFFITWSPSHVNTSVDG 62

Query: 61  RARTLKLDKDSGAAFSSNEMFLFGQMDMKIKLVPGHSAGTVVAYYLTSDQPNRDEIDFEF 120
           R R LKLD DSGA F+SNEMFLFGQMDMKIKLVPGHSAG VVAYYLTSDQPNRDEIDFEF
Sbjct: 63  RTRNLKLDNDSGAGFASNEMFLFGQMDMKIKLVPGHSAGVVVAYYLTSDQPNRDEIDFEF 122

Query: 121 LGNVDGEPYILQTNVFADGFDDREERIKLWFDPTKDFHTYSILWNRYQIVFMVDWVPIRV 180
           LGNV+G+P ILQTNVFADGFDDREERIKLWFDPTKDFHTYSILWN YQIVFMVDWVPIRV
Sbjct: 123 LGNVEGQPIILQTNVFADGFDDREERIKLWFDPTKDFHTYSILWNIYQIVFMVDWVPIRV 182

Query: 181 YRNHADKGVGYPRWQPMSLKVSLWDGDSWATRGGKDKIDWSKGPFVASFGDYKVDACVWR 240
           YRNH DKGVGYPRWQPMSLKVSLW+GDSWATRGGK+KIDWSKGPFVASFGDYKVDAC+WR
Sbjct: 183 YRNHGDKGVGYPRWQPMSLKVSLWNGDSWATRGGKEKIDWSKGPFVASFGDYKVDACIWR 242

Query: 241 GNARLCRGDDHSYWWNLPKYNTLSPPQRRLFKWVRNYHLIYDYCQDNLRFQNHLPKECSL 300
           GNAR CRG+  S WW+LPKYNTLSP QRRLFKWVR Y+LIYDYCQDNLRFQN LPKECSL
Sbjct: 243 GNARFCRGESSSNWWHLPKYNTLSPSQRRLFKWVRKYYLIYDYCQDNLRFQNQLPKECSL 302

Query: 301 PKY 302
           PKY
Sbjct: 303 PKY 304

BLAST of MC10g0327 vs. NCBI nr
Match: CCH26628.1 (putative xyloglucan endotransglucosylase/hydrolase [Cucumis sativus])

HSP 1 Score: 555 bits (1429), Expect = 1.96e-198
Identity = 260/296 (87.84%), Postives = 276/296 (93.24%), Query Frame = 0

Query: 9   MYE-SVKTI-IFFGVFIANLLQFATSSVISTGDFNKDFFITWSPSHVNTSADGRARTLKL 68
           MYE SVKTI IFFGVFI+N LQ + +S+ISTGD+NKDFFITWSPSHVNTS DGRAR LKL
Sbjct: 1   MYECSVKTIMIFFGVFISNFLQLSYASLISTGDYNKDFFITWSPSHVNTSLDGRARNLKL 60

Query: 69  DKDSGAAFSSNEMFLFGQMDMKIKLVPGHSAGTVVAYYLTSDQPNRDEIDFEFLGNVDGE 128
           D DSGA F+SNEMFLFGQMDMKIKLVPG+SAGTVVAYYLTSDQPNRDEIDFEFLGNV+G+
Sbjct: 61  DNDSGAGFASNEMFLFGQMDMKIKLVPGNSAGTVVAYYLTSDQPNRDEIDFEFLGNVEGQ 120

Query: 129 PYILQTNVFADGFDDREERIKLWFDPTKDFHTYSILWNRYQIVFMVDWVPIRVYRNHADK 188
           P ILQTN+FADGFDDREERIKLWFDPTKDFHTYSILWN YQIVFMVDWVPIRVYRNH DK
Sbjct: 121 PIILQTNIFADGFDDREERIKLWFDPTKDFHTYSILWNIYQIVFMVDWVPIRVYRNHGDK 180

Query: 189 GVGYPRWQPMSLKVSLWDGDSWATRGGKDKIDWSKGPFVASFGDYKVDACVWRGNARLCR 248
           GVGYPRWQPMSLKVSLW+G+SWATRGGKDKIDWSKGPFVASFGDYK+DAC+WRGNAR CR
Sbjct: 181 GVGYPRWQPMSLKVSLWNGESWATRGGKDKIDWSKGPFVASFGDYKLDACIWRGNARFCR 240

Query: 249 GDDHSYWWNLPKYNTLSPPQRRLFKWVRNYHLIYDYCQDNLRFQNHLPKECSLPKY 302
           G+  S WW+LPKYNTLSP QRRLFKWVR YHLIYDYCQDNLRF N LPKECSLPKY
Sbjct: 241 GESSSNWWHLPKYNTLSPFQRRLFKWVRKYHLIYDYCQDNLRFHNQLPKECSLPKY 296

BLAST of MC10g0327 vs. NCBI nr
Match: XP_022984549.1 (probable xyloglucan endotransglucosylase/hydrolase protein 10 [Cucurbita maxima])

HSP 1 Score: 542 bits (1396), Expect = 2.03e-193
Identity = 251/295 (85.08%), Postives = 268/295 (90.85%), Query Frame = 0

Query: 9   MYESVKTIIFFGVFIANLLQFATSSVISTGDFNKDFFITWSPSHVNTSADGRARTLKLDK 68
           M+E V+TIIFFGVF A LLQF+++SV+STGD+NKDFF+TWSPSHVNTS DGRAR LKLD 
Sbjct: 1   MFECVQTIIFFGVFFAYLLQFSSASVVSTGDYNKDFFVTWSPSHVNTSVDGRARNLKLDN 60

Query: 69  DSGAAFSSNEMFLFGQMDMKIKLVPGHSAGTVVAYYLTSDQPNRDEIDFEFLGNVDGEPY 128
            SGA F+SNEMFLFGQMDMKIKLVPGHSAG VVAYYLTSDQPN DEIDFEFLGNV+G+P 
Sbjct: 61  YSGAGFASNEMFLFGQMDMKIKLVPGHSAGIVVAYYLTSDQPNHDEIDFEFLGNVEGQPI 120

Query: 129 ILQTNVFADGFDDREERIKLWFDPTKDFHTYSILWNRYQIVFMVDWVPIRVYRNHADKGV 188
           ILQTN+FADGFDDREERI LWFDPTKDFHTYSILWN YQIVFMVDWVPIRVYRNHADKGV
Sbjct: 121 ILQTNIFADGFDDREERINLWFDPTKDFHTYSILWNLYQIVFMVDWVPIRVYRNHADKGV 180

Query: 189 GYPRWQPMSLKVSLWDGDSWATRGGKDKIDWSKGPFVASFGDYKVDACVWRGNARLCRGD 248
           GYPRWQPMSLKVSLWDG SWATRGGKDKI+WSKGPF+ASFGDYK+DAC WRGNAR CRG+
Sbjct: 181 GYPRWQPMSLKVSLWDGSSWATRGGKDKINWSKGPFIASFGDYKLDACTWRGNARFCRGE 240

Query: 249 DHSY-WWNLPKYNTLSPPQRRLFKWVRNYHLIYDYCQDNLRFQNHLPKECSLPKY 302
             S  WWNLPKYNTLS  QRRLFKWVR  HL+YDYCQDN RF N LPKECSLPKY
Sbjct: 241 ISSKNWWNLPKYNTLSSSQRRLFKWVRKNHLVYDYCQDNSRFHNQLPKECSLPKY 295

BLAST of MC10g0327 vs. ExPASy TrEMBL
Match: A0A6J1D7T4 (Xyloglucan endotransglucosylase/hydrolase OS=Momordica charantia OX=3673 GN=LOC111017713 PE=3 SV=1)

HSP 1 Score: 645 bits (1665), Expect = 1.25e-234
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0

Query: 1   MRGDQEKAMYESVKTIIFFGVFIANLLQFATSSVISTGDFNKDFFITWSPSHVNTSADGR 60
           MRGDQEKAMYESVKTIIFFGVFIANLLQFATSSVISTGDFNKDFFITWSPSHVNTSADGR
Sbjct: 1   MRGDQEKAMYESVKTIIFFGVFIANLLQFATSSVISTGDFNKDFFITWSPSHVNTSADGR 60

Query: 61  ARTLKLDKDSGAAFSSNEMFLFGQMDMKIKLVPGHSAGTVVAYYLTSDQPNRDEIDFEFL 120
           ARTLKLDKDSGAAFSSNEMFLFGQMDMKIKLVPGHSAGTVVAYYLTSDQPNRDEIDFEFL
Sbjct: 61  ARTLKLDKDSGAAFSSNEMFLFGQMDMKIKLVPGHSAGTVVAYYLTSDQPNRDEIDFEFL 120

Query: 121 GNVDGEPYILQTNVFADGFDDREERIKLWFDPTKDFHTYSILWNRYQIVFMVDWVPIRVY 180
           GNVDGEPYILQTNVFADGFDDREERIKLWFDPTKDFHTYSILWNRYQIVFMVDWVPIRVY
Sbjct: 121 GNVDGEPYILQTNVFADGFDDREERIKLWFDPTKDFHTYSILWNRYQIVFMVDWVPIRVY 180

Query: 181 RNHADKGVGYPRWQPMSLKVSLWDGDSWATRGGKDKIDWSKGPFVASFGDYKVDACVWRG 240
           RNHADKGVGYPRWQPMSLKVSLWDGDSWATRGGKDKIDWSKGPFVASFGDYKVDACVWRG
Sbjct: 181 RNHADKGVGYPRWQPMSLKVSLWDGDSWATRGGKDKIDWSKGPFVASFGDYKVDACVWRG 240

Query: 241 NARLCRGDDHSYWWNLPKYNTLSPPQRRLFKWVRNYHLIYDYCQDNLRFQNHLPKECSLP 300
           NARLCRGDDHSYWWNLPKYNTLSPPQRRLFKWVRNYHLIYDYCQDNLRFQNHLPKECSLP
Sbjct: 241 NARLCRGDDHSYWWNLPKYNTLSPPQRRLFKWVRNYHLIYDYCQDNLRFQNHLPKECSLP 300

Query: 301 KY 302
           KY
Sbjct: 301 KY 302

BLAST of MC10g0327 vs. ExPASy TrEMBL
Match: A0A1S3BY49 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo OX=3656 GN=LOC103494840 PE=3 SV=1)

HSP 1 Score: 566 bits (1458), Expect = 5.04e-203
Identity = 266/305 (87.21%), Postives = 281/305 (92.13%), Query Frame = 0

Query: 1   MRGDQEKA--MYE-SVKTIIFFGVFIANLLQFATSSVISTGDFNKDFFITWSPSHVNTSA 60
           MRG   KA  MYE SVKTIIFFGVFI+N LQF+ +S ISTGD+NKDFFITWSPSHVNTS 
Sbjct: 1   MRGGNYKAIIMYECSVKTIIFFGVFISNFLQFSFASPISTGDYNKDFFITWSPSHVNTSL 60

Query: 61  DGRARTLKLDKDSGAAFSSNEMFLFGQMDMKIKLVPGHSAGTVVAYYLTSDQPNRDEIDF 120
           DGR+R LKLD DSGA F+SNEMFLFGQMDMKIKLVPG+SAGTVVAYYLTSDQPNRDEIDF
Sbjct: 61  DGRSRNLKLDNDSGAGFASNEMFLFGQMDMKIKLVPGNSAGTVVAYYLTSDQPNRDEIDF 120

Query: 121 EFLGNVDGEPYILQTNVFADGFDDREERIKLWFDPTKDFHTYSILWNRYQIVFMVDWVPI 180
           EFLGNV+G+P ILQTN+FADGFDDREERIKLWFDPTKDFHTYSILWN YQIVFMVDWVPI
Sbjct: 121 EFLGNVEGQPIILQTNIFADGFDDREERIKLWFDPTKDFHTYSILWNIYQIVFMVDWVPI 180

Query: 181 RVYRNHADKGVGYPRWQPMSLKVSLWDGDSWATRGGKDKIDWSKGPFVASFGDYKVDACV 240
           RVYRNH DKGVGYPRWQPMSLKVSLW+G+SWATRGGKDKIDWSKGPFVASFGDYKVDAC+
Sbjct: 181 RVYRNHGDKGVGYPRWQPMSLKVSLWNGESWATRGGKDKIDWSKGPFVASFGDYKVDACI 240

Query: 241 WRGNARLCRGDDHSYWWNLPKYNTLSPPQRRLFKWVRNYHLIYDYCQDNLRFQNHLPKEC 300
           WRGNAR CRG+  S WW+LPKYNTLSP QRRLFKWVR YHLIYDYCQDNLRF N LPKEC
Sbjct: 241 WRGNARFCRGESSSNWWHLPKYNTLSPSQRRLFKWVRKYHLIYDYCQDNLRFHNQLPKEC 300

Query: 301 SLPKY 302
           SLPKY
Sbjct: 301 SLPKY 305

BLAST of MC10g0327 vs. ExPASy TrEMBL
Match: A0A0A0KUY6 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G000010 PE=3 SV=1)

HSP 1 Score: 559 bits (1440), Expect = 3.12e-200
Identity = 265/306 (86.60%), Postives = 281/306 (91.83%), Query Frame = 0

Query: 1   MRGDQEKA--MYE-SVKTI-IFFGVFIANLLQFATSSVISTGDFNKDFFITWSPSHVNTS 60
           MRG   KA  MYE SVKTI IFFGVFI+N LQ + +S+ISTGD+NKDFFITWSPSHVNTS
Sbjct: 3   MRGGDYKAIIMYECSVKTIMIFFGVFISNFLQLSYASLISTGDYNKDFFITWSPSHVNTS 62

Query: 61  ADGRARTLKLDKDSGAAFSSNEMFLFGQMDMKIKLVPGHSAGTVVAYYLTSDQPNRDEID 120
            DGRAR LKLD DSGA F+SNEMFLFGQMDMKIKLVPG+SAGTVVAYYLTSDQPNRDEID
Sbjct: 63  LDGRARNLKLDNDSGAGFASNEMFLFGQMDMKIKLVPGNSAGTVVAYYLTSDQPNRDEID 122

Query: 121 FEFLGNVDGEPYILQTNVFADGFDDREERIKLWFDPTKDFHTYSILWNRYQIVFMVDWVP 180
           FEFLGNV+G+P ILQTN+FADGFDDREERIKLWFDPTKDFHTYSILWN YQIVFMVDWVP
Sbjct: 123 FEFLGNVEGQPIILQTNIFADGFDDREERIKLWFDPTKDFHTYSILWNIYQIVFMVDWVP 182

Query: 181 IRVYRNHADKGVGYPRWQPMSLKVSLWDGDSWATRGGKDKIDWSKGPFVASFGDYKVDAC 240
           IRVYRNH DKGVGYPRWQPMSLKVSLW+G+SWATRGGKDKIDWSKGPFVASFGDYK+DAC
Sbjct: 183 IRVYRNHGDKGVGYPRWQPMSLKVSLWNGESWATRGGKDKIDWSKGPFVASFGDYKLDAC 242

Query: 241 VWRGNARLCRGDDHSYWWNLPKYNTLSPPQRRLFKWVRNYHLIYDYCQDNLRFQNHLPKE 300
           +WRGNAR CRG+  S WW+LPKYNTLSP QRRLFKWVR YHLIYDYCQDNLRF N LPKE
Sbjct: 243 IWRGNARFCRGESSSNWWHLPKYNTLSPFQRRLFKWVRKYHLIYDYCQDNLRFHNQLPKE 302

Query: 301 CSLPKY 302
           CSLPKY
Sbjct: 303 CSLPKY 308

BLAST of MC10g0327 vs. ExPASy TrEMBL
Match: N0DXB4 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth18 PE=3 SV=1)

HSP 1 Score: 555 bits (1429), Expect = 9.51e-199
Identity = 260/296 (87.84%), Postives = 276/296 (93.24%), Query Frame = 0

Query: 9   MYE-SVKTI-IFFGVFIANLLQFATSSVISTGDFNKDFFITWSPSHVNTSADGRARTLKL 68
           MYE SVKTI IFFGVFI+N LQ + +S+ISTGD+NKDFFITWSPSHVNTS DGRAR LKL
Sbjct: 1   MYECSVKTIMIFFGVFISNFLQLSYASLISTGDYNKDFFITWSPSHVNTSLDGRARNLKL 60

Query: 69  DKDSGAAFSSNEMFLFGQMDMKIKLVPGHSAGTVVAYYLTSDQPNRDEIDFEFLGNVDGE 128
           D DSGA F+SNEMFLFGQMDMKIKLVPG+SAGTVVAYYLTSDQPNRDEIDFEFLGNV+G+
Sbjct: 61  DNDSGAGFASNEMFLFGQMDMKIKLVPGNSAGTVVAYYLTSDQPNRDEIDFEFLGNVEGQ 120

Query: 129 PYILQTNVFADGFDDREERIKLWFDPTKDFHTYSILWNRYQIVFMVDWVPIRVYRNHADK 188
           P ILQTN+FADGFDDREERIKLWFDPTKDFHTYSILWN YQIVFMVDWVPIRVYRNH DK
Sbjct: 121 PIILQTNIFADGFDDREERIKLWFDPTKDFHTYSILWNIYQIVFMVDWVPIRVYRNHGDK 180

Query: 189 GVGYPRWQPMSLKVSLWDGDSWATRGGKDKIDWSKGPFVASFGDYKVDACVWRGNARLCR 248
           GVGYPRWQPMSLKVSLW+G+SWATRGGKDKIDWSKGPFVASFGDYK+DAC+WRGNAR CR
Sbjct: 181 GVGYPRWQPMSLKVSLWNGESWATRGGKDKIDWSKGPFVASFGDYKLDACIWRGNARFCR 240

Query: 249 GDDHSYWWNLPKYNTLSPPQRRLFKWVRNYHLIYDYCQDNLRFQNHLPKECSLPKY 302
           G+  S WW+LPKYNTLSP QRRLFKWVR YHLIYDYCQDNLRF N LPKECSLPKY
Sbjct: 241 GESSSNWWHLPKYNTLSPFQRRLFKWVRKYHLIYDYCQDNLRFHNQLPKECSLPKY 296

BLAST of MC10g0327 vs. ExPASy TrEMBL
Match: A0A6J1JAU9 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111482810 PE=3 SV=1)

HSP 1 Score: 542 bits (1396), Expect = 9.81e-194
Identity = 251/295 (85.08%), Postives = 268/295 (90.85%), Query Frame = 0

Query: 9   MYESVKTIIFFGVFIANLLQFATSSVISTGDFNKDFFITWSPSHVNTSADGRARTLKLDK 68
           M+E V+TIIFFGVF A LLQF+++SV+STGD+NKDFF+TWSPSHVNTS DGRAR LKLD 
Sbjct: 1   MFECVQTIIFFGVFFAYLLQFSSASVVSTGDYNKDFFVTWSPSHVNTSVDGRARNLKLDN 60

Query: 69  DSGAAFSSNEMFLFGQMDMKIKLVPGHSAGTVVAYYLTSDQPNRDEIDFEFLGNVDGEPY 128
            SGA F+SNEMFLFGQMDMKIKLVPGHSAG VVAYYLTSDQPN DEIDFEFLGNV+G+P 
Sbjct: 61  YSGAGFASNEMFLFGQMDMKIKLVPGHSAGIVVAYYLTSDQPNHDEIDFEFLGNVEGQPI 120

Query: 129 ILQTNVFADGFDDREERIKLWFDPTKDFHTYSILWNRYQIVFMVDWVPIRVYRNHADKGV 188
           ILQTN+FADGFDDREERI LWFDPTKDFHTYSILWN YQIVFMVDWVPIRVYRNHADKGV
Sbjct: 121 ILQTNIFADGFDDREERINLWFDPTKDFHTYSILWNLYQIVFMVDWVPIRVYRNHADKGV 180

Query: 189 GYPRWQPMSLKVSLWDGDSWATRGGKDKIDWSKGPFVASFGDYKVDACVWRGNARLCRGD 248
           GYPRWQPMSLKVSLWDG SWATRGGKDKI+WSKGPF+ASFGDYK+DAC WRGNAR CRG+
Sbjct: 181 GYPRWQPMSLKVSLWDGSSWATRGGKDKINWSKGPFIASFGDYKLDACTWRGNARFCRGE 240

Query: 249 DHSY-WWNLPKYNTLSPPQRRLFKWVRNYHLIYDYCQDNLRFQNHLPKECSLPKY 302
             S  WWNLPKYNTLS  QRRLFKWVR  HL+YDYCQDN RF N LPKECSLPKY
Sbjct: 241 ISSKNWWNLPKYNTLSSSQRRLFKWVRKNHLVYDYCQDNSRFHNQLPKECSLPKY 295

BLAST of MC10g0327 vs. TAIR 10
Match: AT2G14620.1 (xyloglucan endotransglucosylase/hydrolase 10 )

HSP 1 Score: 458.4 bits (1178), Expect = 4.5e-129
Identity = 203/287 (70.73%), Postives = 244/287 (85.02%), Query Frame = 0

Query: 16  IIFFGVFIANLLQFATSSVISTGDFNKDFFITWSPSHVNTSADGRARTLKLDKDSGAAFS 75
           ++ F +  + LL  + +SV+S+GDFNKDFF+TWSP+HVNTS DGR+RTLKLD++SGA+FS
Sbjct: 13  LVGFSIISSLLLWVSQASVVSSGDFNKDFFVTWSPTHVNTSNDGRSRTLKLDQESGASFS 72

Query: 76  SNEMFLFGQMDMKIKLVPGHSAGTVVAYYLTSDQPNRDEIDFEFLGNVDGEPYILQTNVF 135
           S + FLFGQ+DMKIKL+ G S GTVVAYY++SDQPNRDEIDFEFLGNV+G+PYILQTNV+
Sbjct: 73  SIQTFLFGQIDMKIKLIRGSSQGTVVAYYMSSDQPNRDEIDFEFLGNVNGQPYILQTNVY 132

Query: 136 ADGFDDREERIKLWFDPTKDFHTYSILWNRYQIVFMVDWVPIRVYRNHADKGVGYPRWQP 195
           A+G D+REERI LWFDP KDFHTYSILWN +QIVFMVD +PIR+YRNH +KGV YPR QP
Sbjct: 133 AEGLDNREERIHLWFDPAKDFHTYSILWNIHQIVFMVDQIPIRLYRNHGEKGVAYPRLQP 192

Query: 196 MSLKVSLWDGDSWATRGGKDKIDWSKGPFVASFGDYKVDACVWRGNARLCRGDDHSYWWN 255
           MS++ SLW+G+SWATRGG DKIDWSKGPFVASFGDYK+DAC+W GN   C G+    WWN
Sbjct: 193 MSVQASLWNGESWATRGGHDKIDWSKGPFVASFGDYKIDACIWIGNTSFCNGESTENWWN 252

Query: 256 LPKYNTLSPPQRRLFKWVRNYHLIYDYCQDNLRFQNHLPKECSLPKY 303
             ++++L+  Q+R FKWVR YHLIYDYCQD  RF N LPKECSLPKY
Sbjct: 253 KNEFSSLTRVQKRWFKWVRKYHLIYDYCQDYGRFNNKLPKECSLPKY 299

BLAST of MC10g0327 vs. TAIR 10
Match: AT4G37800.1 (xyloglucan endotransglucosylase/hydrolase 7 )

HSP 1 Score: 288.1 bits (736), Expect = 8.1e-78
Identity = 135/260 (51.92%), Postives = 177/260 (68.08%), Query Frame = 0

Query: 40  FNKDFFITWSPSHVNTSADGRARTLKLDKDSGAAFSSNEMFLFGQMDMKIKLVPGHSAGT 99
           F  DF I WS +H+     GRA  LKLD  SG  F+S + +LFG++ MKIKL+PG SAGT
Sbjct: 34  FEDDFRIAWSDTHITQIDGGRAIQLKLDPSSGCGFASKKQYLFGRVSMKIKLIPGDSAGT 93

Query: 100 VVAYYLTSDQPN-RDEIDFEFLGNVDGEPYILQTNVFADGFDDREERIKLWFDPTKDFHT 159
           V A+Y+ SD  + RDE+DFEFLGN  G+PY +QTNVFA G  DRE+R+ LWFDP++DFH 
Sbjct: 94  VTAFYMNSDTDSVRDELDFEFLGNRSGQPYTVQTNVFAHGKGDREQRVNLWFDPSRDFHE 153

Query: 160 YSILWNRYQIVFMVDWVPIRVYRNHADKGVGYPRWQPMSLKVSLWDGDSWATRGGKDKID 219
           Y+I WN  +IVF VD VPIRVY+N+  + V YPR+QPM +  +LW+ D WATRGG +KI+
Sbjct: 154 YAISWNHLRIVFYVDNVPIRVYKNNEARKVPYPRFQPMGVYSTLWEADDWATRGGIEKIN 213

Query: 220 WSKGPFVASFGDYKVDACVWRGNARLCRGDDHSYWWNLPKYNTLSPPQRRLFKWVRNYHL 279
           WS+ PF A + D+ ++ C   G A  C  +  + WW    Y+ LSP + R ++WVR  H+
Sbjct: 214 WSRAPFYAYYKDFDIEGCPVPGPAD-CPANSKN-WWEGSAYHQLSPVEARSYRWVRVNHM 273

Query: 280 IYDYCQDNLRFQNHLPKECS 299
           +YDYC D  RF    P ECS
Sbjct: 274 VYDYCTDKSRFPVP-PPECS 290

BLAST of MC10g0327 vs. TAIR 10
Match: AT5G65730.1 (xyloglucan endotransglucosylase/hydrolase 6 )

HSP 1 Score: 279.6 bits (714), Expect = 2.9e-75
Identity = 129/259 (49.81%), Postives = 171/259 (66.02%), Query Frame = 0

Query: 40  FNKDFFITWSPSHVNTSADGRARTLKLDKDSGAAFSSNEMFLFGQMDMKIKLVPGHSAGT 99
           F +DF   WS SH+    DG+A  L LD+ +G  F+S   +LFG++ MKIKL+PG SAGT
Sbjct: 35  FVEDFKAAWSESHIRQMEDGKAIQLVLDQSTGCGFASKRKYLFGRVSMKIKLIPGDSAGT 94

Query: 100 VVAYYLTSDQPN-RDEIDFEFLGNVDGEPYILQTNVFADGFDDREERIKLWFDPTKDFHT 159
           V A+Y+ SD    RDE+DFEFLGN  G+PY +QTN+FA G  DRE+R+ LWFDP+ D+HT
Sbjct: 95  VTAFYMNSDTATVRDELDFEFLGNRSGQPYSVQTNIFAHGKGDREQRVNLWFDPSMDYHT 154

Query: 160 YSILWNRYQIVFMVDWVPIRVYRNHADKGVGYPRWQPMSLKVSLWDGDSWATRGGKDKID 219
           Y+ILW+   IVF VD VPIR Y+N+  K + YP  QPM +  +LW+ D WATRGG +KID
Sbjct: 155 YTILWSHKHIVFYVDDVPIREYKNNEAKNIAYPTSQPMGVYSTLWEADDWATRGGLEKID 214

Query: 220 WSKGPFVASFGDYKVDACVWRGNARLCRGDDHSYWWNLPKYNTLSPPQRRLFKWVRNYHL 279
           WSK PF A + D+ ++ C   G    C  + H+ WW    Y +L+  + R ++WVR  H+
Sbjct: 215 WSKAPFYAYYKDFDIEGCPVPG-PTFCPSNPHN-WWEGYAYQSLNAVEARRYRWVRVNHM 274

Query: 280 IYDYCQDNLRFQNHLPKEC 298
           +YDYC D  RF    P EC
Sbjct: 275 VYDYCTDRSRFPVP-PPEC 290

BLAST of MC10g0327 vs. TAIR 10
Match: AT5G57530.1 (xyloglucan endotransglucosylase/hydrolase 12 )

HSP 1 Score: 278.5 bits (711), Expect = 6.4e-75
Identity = 133/270 (49.26%), Postives = 173/270 (64.07%), Query Frame = 0

Query: 35  ISTGDFNKDFFITWSPSHVNTSADGRARTLKLDKDSGAAFSSNEMFLFGQMDMKIKLVPG 94
           ++TG F   F ITW     N    G+  T  LDK SG+ F S + +LFG++DMKIKLVPG
Sbjct: 22  VATGSFYDSFDITWGAGRANIFESGQLLTCTLDKTSGSGFQSKKEYLFGKIDMKIKLVPG 81

Query: 95  HSAGTVVAYYLTSDQPNRDEIDFEFLGNVDGEPYILQTNVFADGFDDREERIKLWFDPTK 154
           +SAGTV AYYL+S     DEIDFEFLGNV G+PY++ TNVF  G  +RE +  LWFDPT 
Sbjct: 82  NSAGTVTAYYLSSKGETWDEIDFEFLGNVTGQPYVIHTNVFTGGKGNREMQFYLWFDPTA 141

Query: 155 DFHTYSILWNRYQIVFMVDWVPIRVYRNHADKGVGYPRWQPMSLKVSLWDGDSWATRGGK 214
           DFHTY++LWN   I+F+VD +PIRV++N+   GV YP+ QPM +  SLW+ D WAT+GGK
Sbjct: 142 DFHTYTVLWNPLNIIFLVDGIPIRVFKNNEANGVAYPKSQPMKIYSSLWEADDWATQGGK 201

Query: 215 DKIDWSKGPFVASFGDYK-VDAC----VWRGNARLCRGDDHSYWWNLPKYNTLSPPQRRL 274
            K DW+  PF AS+  +  VD C    +W  N   C  + +S+ W      TL+  Q   
Sbjct: 202 VKTDWTNAPFSASYRSFNDVDCCSRTSIW--NWVTCNANSNSWMW-----TTLNSNQLGQ 261

Query: 275 FKWVRNYHLIYDYCQDNLRFQNHLPKECSL 300
            KWV+  ++IY+YC D  RF   LP EC+L
Sbjct: 262 LKWVQKDYMIYNYCTDFKRFPQGLPTECNL 284

BLAST of MC10g0327 vs. TAIR 10
Match: AT1G11545.1 (xyloglucan endotransglucosylase/hydrolase 8 )

HSP 1 Score: 277.7 bits (709), Expect = 1.1e-74
Identity = 137/302 (45.36%), Postives = 184/302 (60.93%), Query Frame = 0

Query: 4   DQEKAMYESVKTIIFFGVFIANLLQFATSSVISTGDFNKDFFITWSPSHVNTSADGRART 63
           + E+ +  S   +    +F+  L+  ++ +   T  F  +F I WS +H  TS DG    
Sbjct: 2   ETERRIITSCSAMTALFLFMTALMASSSIAATPTQSFEDNFNIMWSENHFTTSDDGEIWN 61

Query: 64  LKLDKDSGAAFSSNEMFLFGQMDMKIKLVPGHSAGTVVAYYLTSDQ---PNRDEIDFEFL 123
           L LD D+G  F +  M+ FG   MK+KLV G SAG V AYY+ S+    P RDEIDFEFL
Sbjct: 62  LSLDNDTGCGFQTKHMYRFGWFSMKLKLVGGDSAGVVTAYYMCSENGAGPERDEIDFEFL 121

Query: 124 GNVDGEPYILQTNVFADGFDDREERIKLWFDPTKDFHTYSILWNRYQIVFMVDWVPIRVY 183
           GN  G+PYI+QTNV+ +G  +RE R  LWFDPTKD+HTYSILWN +Q+VF VD VPIRVY
Sbjct: 122 GNRTGQPYIIQTNVYKNGTGNREMRHSLWFDPTKDYHTYSILWNNHQLVFFVDRVPIRVY 181

Query: 184 RNHADKGVG---YPRWQPMSLKVSLWDGDSWATRGGKDKIDWSKGPFVASFGDYKVDACV 243
           +N +DK      +P  +PM L  S+W+ D WATRGG +K DW K PFV+S+ D+ V+ C 
Sbjct: 182 KN-SDKVPNNDFFPNQKPMYLFSSIWNADDWATRGGLEKTDWKKAPFVSSYKDFAVEGCR 241

Query: 244 WRGNARLCRGDDHSYWWNLPKYNTLSPPQRRLFKWVRNYHLIYDYCQDNLRFQNHLPKEC 300
           W+     C       WW+      LS  Q+  + WV+   ++YDYC+D+ RF   LP EC
Sbjct: 242 WKDPFPACVSTTTENWWDQYDAWHLSKTQKMDYAWVQRNLVVYDYCKDSERFPT-LPWEC 301

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZVK16.4e-12870.73Probable xyloglucan endotransglucosylase/hydrolase protein 10 OS=Arabidopsis tha... [more]
Q8LER31.1e-7651.92Probable xyloglucan endotransglucosylase/hydrolase protein 7 OS=Arabidopsis thal... [more]
Q415421.3e-7551.94Probable xyloglucan endotransglucosylase/hydrolase OS=Triticum aestivum OX=4565 ... [more]
Q8LF994.1e-7449.81Probable xyloglucan endotransglucosylase/hydrolase protein 6 OS=Arabidopsis thal... [more]
Q9FKL99.1e-7449.26Probable xyloglucan endotransglucosylase/hydrolase protein 12 OS=Arabidopsis tha... [more]
Match NameE-valueIdentityDescription
XP_022149246.12.58e-234100.00probable xyloglucan endotransglucosylase/hydrolase protein 10 [Momordica charant... [more]
XP_008454432.11.04e-20287.21PREDICTED: probable xyloglucan endotransglucosylase/hydrolase protein 10 [Cucumi... [more]
XP_038905402.11.66e-20186.80probable xyloglucan endotransglucosylase/hydrolase protein 10 [Benincasa hispida... [more]
CCH26628.11.96e-19887.84putative xyloglucan endotransglucosylase/hydrolase [Cucumis sativus][more]
XP_022984549.12.03e-19385.08probable xyloglucan endotransglucosylase/hydrolase protein 10 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1D7T41.25e-234100.00Xyloglucan endotransglucosylase/hydrolase OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A1S3BY495.04e-20387.21Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo OX=3656 GN=LOC10349484... [more]
A0A0A0KUY63.12e-20086.60Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_4G00... [more]
N0DXB49.51e-19987.84Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth18 PE... [more]
A0A6J1JAU99.81e-19485.08Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT2G14620.14.5e-12970.73xyloglucan endotransglucosylase/hydrolase 10 [more]
AT4G37800.18.1e-7851.92xyloglucan endotransglucosylase/hydrolase 7 [more]
AT5G65730.12.9e-7549.81xyloglucan endotransglucosylase/hydrolase 6 [more]
AT5G57530.16.4e-7549.26xyloglucan endotransglucosylase/hydrolase 12 [more]
AT1G11545.11.1e-7445.36xyloglucan endotransglucosylase/hydrolase 8 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016455Xyloglucan endotransglucosylase/hydrolasePIRSFPIRSF005604EndGlu_transfcoord: 5..302
e-value: 2.0E-114
score: 379.6
IPR016455Xyloglucan endotransglucosylase/hydrolaseCDDcd02176GH16_XETcoord: 36..297
e-value: 2.02924E-144
score: 405.044
IPR010713Xyloglucan endo-transglycosylase, C-terminalPFAMPF06955XET_Ccoord: 251..297
e-value: 1.4E-15
score: 56.8
IPR000757Glycoside hydrolase family 16PFAMPF00722Glyco_hydro_16coord: 41..221
e-value: 2.4E-55
score: 186.8
IPR000757Glycoside hydrolase family 16PROSITEPS51762GH16_2coord: 30..228
score: 27.517635
NoneNo IPR availableGENE3D2.60.120.200coord: 27..301
e-value: 4.1E-95
score: 320.2
NoneNo IPR availablePANTHERPTHR31062:SF120XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 10-RELATEDcoord: 15..300
IPR044791Beta-glucanase/XTHPANTHERPTHR31062XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATEDcoord: 15..300
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 39..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g0327.1MC10g0327.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042546 cell wall biogenesis
biological_process GO:0071555 cell wall organization
biological_process GO:0010411 xyloglucan metabolic process
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006073 cellular glucan metabolic process
cellular_component GO:0048046 apoplast
cellular_component GO:0005618 cell wall
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0016762 xyloglucan:xyloglucosyl transferase activity