MC10g0166 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g0166
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionPeroxidase
LocationMC10: 1177379 .. 1178968 (+)
RNA-Seq ExpressionMC10g0166
SyntenyMC10g0166
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTGGACTAGAGAATGATATTAAAAAAACAGCAACAGCATGGTTGCTTAAGGAATTCCCAAAGTTGGAAAAGAGAAAAAGTGGGGAGTACAGCTGATTGTGTCCTCTTCCACAATGGAGTAGCTTGAAAGTACAGTAGGATCAATCCAAATCAACACAACTAATTTGCTTCATTAACCATTCATTAACTTAATTCTTTCCCATTCTTCATAAATCTCTATATAAAGAGATGATCAAACAGATATATGCCAAACCAAAATACGTTTCATACAATACAAATGGCGACAAATGCAAGAGCAGTCTTGTTTCTAATGCTCATGCTCGTGGTTTGTGTATCTGAAGGAGTTCTTGACGGCAATTATTACACAAAAACATGTCCAAATGCTGAGAAAATCATCCTCCAGACCGTTTACAATGCTTCCATTCATGACCCCCGAGTCCCTCCTCGGCTTCTCAGGCTCTTCTTCCATGACTGTTTCATAAGGGTAATCGTAAATCTCACTAAAACTTTCTCAAGTGTTAGAATTAATGCAATGGGATTGGTGTTTGACAGGGATGTGATGCATCAGTGTTGATAGATTCAACGCCGGGGAAGCAGGCCGAGAAAGATGCACCTCCAAACATCTCGCTGAGGTCATTCTACGTGATCGACGAGGCCAAGGTGAAGCTGGAAACTGCTTGCCCGCGTACTGTTTCCTGTGCTGATATTGTCGCCATTGCTGCTAGAGATGTAGTAACTCTGGTACTCAACTCATCTTCCTCCCACCAGTTAGTTGAGGCCTATTAATGTTTAGCATCAGTGATATTCTCTTTGCTTTCCTGATTTCTTGGAAAAATATGCAGTCTGGGGGACCATATTGGAGTGTACTGAAAGGAAGGAAAGATGGGAAGATTTCCAATGCTTCTGAAACCATCAACTTGCCAGCTCCAACTTTCAATGTTTCTCAACTCATCCAAAGCTTTTCAAACAGGGGATTACAAGTCAAAGACTTGGTTGCTCTATCTGGGGCTCACACTCTTGGATTCTCACACTGTTCTTCCTTCCAATCCAGACTCCGCAACTTCACCTCAATTCATCAAACTGATCCAACTCTGGAATCTGGGTTTGCGCAGATTCTAAGAGAGAAATGTCCAAAACCCAATTTGGACAAAAATGCTGGACAATTCTTGGACCCAACTTCCTCAACTTTTGACAATGTGTATTACAAGCGACTGATGGAAGGAAAGGGTGTGTTTGGTTCAGATCAAGCTCTGTTTGGTGATTCCAGAACCAGAAGGATTGTTGGGTCGTTTGCTAAAGATCAGAATTTGTTCTTCAAGGAGTTTTCAGCTTCAATGGTAAAATTGGGTAATGTTGGGGTCAGTGAAAACGGCGATGTCAGAGTCAATTGCAGGGTGGTGAACTGAGAGAAACAGAGAGAGTTTTTTTTACAAAAAATTATGTAATGTTGATGAATTTATACTGTTTGAAATAAAGTGATAAAGATGCCTCTTCCATTGATAGCCAAGAAAGTCCTCAAATATAGTATGTCATATATCAGAGATCAAGTTCACAGAAGAATGTAAAGAACAAGCTCACTAAACAGTAAGG

mRNA sequence

CTTGGACTAGAGAATGATATTAAAAAAACAGCAACAGCATGGTTGCTTAAGGAATTCCCAAAGTTGGAAAAGAGAAAAAGTGGGGAGTACAGCTGATTGTGTCCTCTTCCACAATGGAGTAGCTTGAAAGTACAGTAGGATCAATCCAAATCAACACAACTAATTTGCTTCATTAACCATTCATTAACTTAATTCTTTCCCATTCTTCATAAATCTCTATATAAAGAGATGATCAAACAGATATATGCCAAACCAAAATACGTTTCATACAATACAAATGGCGACAAATGCAAGAGCAGTCTTGTTTCTAATGCTCATGCTCGTGGTTTGTGTATCTGAAGGAGTTCTTGACGGCAATTATTACACAAAAACATGTCCAAATGCTGAGAAAATCATCCTCCAGACCGTTTACAATGCTTCCATTCATGACCCCCGAGTCCCTCCTCGGCTTCTCAGGCTCTTCTTCCATGACTGTTTCATAAGGGGATGTGATGCATCAGTGTTGATAGATTCAACGCCGGGGAAGCAGGCCGAGAAAGATGCACCTCCAAACATCTCGCTGAGGTCATTCTACGTGATCGACGAGGCCAAGGTGAAGCTGGAAACTGCTTGCCCGCGTACTGTTTCCTGTGCTGATATTGTCGCCATTGCTGCTAGAGATGTAGTAACTCTGTCTGGGGGACCATATTGGAGTGTACTGAAAGGAAGGAAAGATGGGAAGATTTCCAATGCTTCTGAAACCATCAACTTGCCAGCTCCAACTTTCAATGTTTCTCAACTCATCCAAAGCTTTTCAAACAGGGGATTACAAGTCAAAGACTTGGTTGCTCTATCTGGGGCTCACACTCTTGGATTCTCACACTGTTCTTCCTTCCAATCCAGACTCCGCAACTTCACCTCAATTCATCAAACTGATCCAACTCTGGAATCTGGGTTTGCGCAGATTCTAAGAGAGAAATGTCCAAAACCCAATTTGGACAAAAATGCTGGACAATTCTTGGACCCAACTTCCTCAACTTTTGACAATGTGTATTACAAGCGACTGATGGAAGGAAAGGGTGTGTTTGGTTCAGATCAAGCTCTGTTTGGTGATTCCAGAACCAGAAGGATTGTTGGGTCGTTTGCTAAAGATCAGAATTTGTTCTTCAAGGAGTTTTCAGCTTCAATGGTAAAATTGGGTAATGTTGGGGTCAGTGAAAACGGCGATGTCAGAGTCAATTGCAGGGTGGTGAACTGAGAGAAACAGAGAGAGTTTTTTTTACAAAAAATTATGTAATGTTGATGAATTTATACTGTTTGAAATAAAGTGATAAAGATGCCTCTTCCATTGATAGCCAAGAAAGTCCTCAAATATAGTATGTCATATATCAGAGATCAAGTTCACAGAAGAATGTAAAGAACAAGCTCACTAAACAGTAAGG

Coding sequence (CDS)

ATGCCAAACCAAAATACGTTTCATACAATACAAATGGCGACAAATGCAAGAGCAGTCTTGTTTCTAATGCTCATGCTCGTGGTTTGTGTATCTGAAGGAGTTCTTGACGGCAATTATTACACAAAAACATGTCCAAATGCTGAGAAAATCATCCTCCAGACCGTTTACAATGCTTCCATTCATGACCCCCGAGTCCCTCCTCGGCTTCTCAGGCTCTTCTTCCATGACTGTTTCATAAGGGGATGTGATGCATCAGTGTTGATAGATTCAACGCCGGGGAAGCAGGCCGAGAAAGATGCACCTCCAAACATCTCGCTGAGGTCATTCTACGTGATCGACGAGGCCAAGGTGAAGCTGGAAACTGCTTGCCCGCGTACTGTTTCCTGTGCTGATATTGTCGCCATTGCTGCTAGAGATGTAGTAACTCTGTCTGGGGGACCATATTGGAGTGTACTGAAAGGAAGGAAAGATGGGAAGATTTCCAATGCTTCTGAAACCATCAACTTGCCAGCTCCAACTTTCAATGTTTCTCAACTCATCCAAAGCTTTTCAAACAGGGGATTACAAGTCAAAGACTTGGTTGCTCTATCTGGGGCTCACACTCTTGGATTCTCACACTGTTCTTCCTTCCAATCCAGACTCCGCAACTTCACCTCAATTCATCAAACTGATCCAACTCTGGAATCTGGGTTTGCGCAGATTCTAAGAGAGAAATGTCCAAAACCCAATTTGGACAAAAATGCTGGACAATTCTTGGACCCAACTTCCTCAACTTTTGACAATGTGTATTACAAGCGACTGATGGAAGGAAAGGGTGTGTTTGGTTCAGATCAAGCTCTGTTTGGTGATTCCAGAACCAGAAGGATTGTTGGGTCGTTTGCTAAAGATCAGAATTTGTTCTTCAAGGAGTTTTCAGCTTCAATGGTAAAATTGGGTAATGTTGGGGTCAGTGAAAACGGCGATGTCAGAGTCAATTGCAGGGTGGTGAACTGA

Protein sequence

MPNQNTFHTIQMATNARAVLFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLRLFFHDCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCADIVAIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVKDLVALSGAHTLGFSHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQFLDPTSSTFDNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKLGNVGVSENGDVRVNCRVVN
Homology
BLAST of MC10g0166 vs. ExPASy Swiss-Prot
Match: Q9LT91 (Peroxidase 66 OS=Arabidopsis thaliana OX=3702 GN=PER66 PE=2 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 4.5e-127
Identity = 219/319 (68.65%), Postives = 262/319 (82.13%), Query Frame = 0

Query: 16  ARAVLFLMLMLVVCV---SEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLRL 75
           ++ ++F M+  V+ +   SE  LD +YY ++CP AEKIIL+TV NA+++DP+VP RLLR+
Sbjct: 4   SKGLIFAMIFAVLAIVKPSEAALDAHYYDQSCPAAEKIILETVRNATLYDPKVPARLLRM 63

Query: 76  FFHDCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCADI 135
           FFHDCFIRGCDAS+L+DST   QAEKD PPNIS+RSFYVI++AK KLE ACPRTVSCAD+
Sbjct: 64  FFHDCFIRGCDASILLDSTRSNQAEKDGPPNISVRSFYVIEDAKRKLEKACPRTVSCADV 123

Query: 136 VAIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVKD 195
           +AIAARDVVTLSGGPYWSVLKGRKDG IS A+ET NLP PTFNVSQLIQSF+ RGL VKD
Sbjct: 124 IAIAARDVVTLSGGPYWSVLKGRKDGTISRANETRNLPPPTFNVSQLIQSFAARGLSVKD 183

Query: 196 LVALSGAHTLGFSHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPK-PNLDKNAGQF 255
           +V LSG HT+GFSHCSSF+SRL+NF+  H  DP++   FAQ L++KCP+  N  KNAG  
Sbjct: 184 MVTLSGGHTIGFSHCSSFESRLQNFSKFHDIDPSMNYAFAQTLKKKCPRTSNRGKNAGTV 243

Query: 256 LDPTSSTFDNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKL 315
           LD TSS FDNVYYK+++ GKGVFGSDQAL GDSRT+ IV +FA+DQ  FF+EF+ASMVKL
Sbjct: 244 LDSTSSVFDNVYYKQILSGKGVFGSDQALLGDSRTKWIVETFAQDQKAFFREFAASMVKL 303

Query: 316 GNVGVSENGDVRVNCRVVN 331
           GN GV E G VRVN R VN
Sbjct: 304 GNFGVKETGQVRVNTRFVN 322

BLAST of MC10g0166 vs. ExPASy Swiss-Prot
Match: Q43872 (Peroxidase 64 OS=Arabidopsis thaliana OX=3702 GN=PER64 PE=1 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 5.8e-90
Identity = 163/310 (52.58%), Postives = 209/310 (67.42%), Query Frame = 0

Query: 20  LFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLRLFFHDCFI 79
           L ++++ VV      L  +YY  TCP A+ I+   V  A  +D  VP  LLR+ FHDCF+
Sbjct: 8   LLVIVIFVVSFDVQALSPHYYDHTCPQADHIVTNAVKKAMSNDQTVPAALLRMHFHDCFV 67

Query: 80  RGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCADIVAIAARD 139
           RGCD SVL+DS    +AEKD PPNISL +FYVID AK  LE  CP  VSCADI+++AARD
Sbjct: 68  RGCDGSVLLDSKGKNKAEKDGPPNISLHAFYVIDNAKKALEEQCPGIVSCADILSLAARD 127

Query: 140 VVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVKDLVALSGA 199
            V LSGGP W+V KGRKDG+IS A ET  LPAPTFN+SQL Q+F  RGL + DLVALSG 
Sbjct: 128 AVALSGGPTWAVPKGRKDGRISKAIETRQLPAPTFNISQLRQNFGQRGLSMHDLVALSGG 187

Query: 200 HTLGFSHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQFLDPTSSTF 259
           HTLGF+HCSSFQ+RL  F +  + DPTL   FA  L   CP  N  KNAG  +D T ++F
Sbjct: 188 HTLGFAHCSSFQNRLHKFNTQKEVDPTLNPSFAARLEGVCPAHNTVKNAGSNMDGTVTSF 247

Query: 260 DNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKLGNVGVSEN 319
           DN+YYK L++GK +F SD++L     T+++V  +A     F + F  SM+K+ ++  + N
Sbjct: 248 DNIYYKMLIQGKSLFSSDESLLAVPSTKKLVAKYANSNEEFERAFVKSMIKMSSISGNGN 307

Query: 320 GDVRVNCRVV 330
            +VR+NCR V
Sbjct: 308 -EVRLNCRRV 316

BLAST of MC10g0166 vs. ExPASy Swiss-Prot
Match: Q9SZB9 (Peroxidase 47 OS=Arabidopsis thaliana OX=3702 GN=PER47 PE=2 SV=2)

HSP 1 Score: 281.2 bits (718), Expect = 1.5e-74
Identity = 146/298 (48.99%), Postives = 188/298 (63.09%), Query Frame = 0

Query: 35  LDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLRLFFHDCFIRGCDASVLIDSTPGK 94
           L   YY  +CP AE+I+  +V NA   DP +   L+R+ FHDCFI GCDAS+L+DST   
Sbjct: 37  LSMTYYMMSCPFAEQIVKNSVNNALQADPTLAAGLIRMLFHDCFIEGCDASILLDSTKDN 96

Query: 95  QAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCADIVAIAARDVVTLSGGPYWSVLKG 154
            AEKD+P N+SLR + +ID+AK K+E  CP  VSCADIVA+AARD V  +GGPY+ + KG
Sbjct: 97  TAEKDSPANLSLRGYEIIDDAKEKIENRCPGVVSCADIVAMAARDAVFWAGGPYYDIPKG 156

Query: 155 RKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVKDLVALSGAHTLGFSHCSSFQSRL 214
           R DGK S   +T NLP+P  N SQLIQ+F  RG   +D+VALSGAHTLG + CSSF++RL
Sbjct: 157 RFDGKRSKIEDTRNLPSPFLNASQLIQTFGQRGFTPQDVVALSGAHTLGVARCSSFKARL 216

Query: 215 RNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQFLDPTSSTFDNVYYKRLMEGKGVF 274
                    D +L+S FA  L + C   +   NA Q  D T + FDN Y+  L    GV 
Sbjct: 217 T------VPDSSLDSTFANTLSKTC---SAGDNAEQPFDATRNDFDNAYFNALQMKSGVL 276

Query: 275 GSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKLGNVGV--SENGDVRVNCRVVN 331
            SDQ LF   RTR +V  +A +Q  FF +F  +M K+ N+ V     G+VR NCR +N
Sbjct: 277 FSDQTLFNTPRTRNLVNGYALNQAKFFFDFQQAMRKMSNLDVKLGSQGEVRQNCRSIN 325

BLAST of MC10g0166 vs. ExPASy Swiss-Prot
Match: Q9LSY7 (Peroxidase 30 OS=Arabidopsis thaliana OX=3702 GN=PER30 PE=1 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.1e-72
Identity = 150/326 (46.01%), Postives = 208/326 (63.80%), Query Frame = 0

Query: 9   TIQMATNARAVLFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPR 68
           T+     A  V+  +L+ ++  SE  L  N+Y K+CPNAEKII   + N   + P +   
Sbjct: 3   TMTQLNIAVVVVVTVLIGMLRSSEAQLQMNFYAKSCPNAEKIISDHIQNHIHNGPSLAAP 62

Query: 69  LLRLFFHDCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVS 128
           L+R+ FHDCF+RGCD SVLI+ST G  AE+DAPPN++LR F  ++  K  LE  CP+TVS
Sbjct: 63  LIRMHFHDCFVRGCDGSVLINSTSG-NAERDAPPNLTLRGFGFVERIKALLEKVCPKTVS 122

Query: 129 CADIVAIAARDVVTLSGGPYWSVLKGRKDGKISNASE-TINLPAPTFNVSQLIQSFSNRG 188
           CADI+A+ ARD V  +GGP WSV  GR+DG+ISN +E T N+P PT N + L + F N+G
Sbjct: 123 CADIIALTARDAVVATGGPSWSVPTGRRDGRISNKTEATNNIPPPTSNFTTLQRLFKNQG 182

Query: 189 LQVKDLVALSGAHTLGFSHCSSFQSRLRNFTSIHQTDPTLESGFAQILR-EKCPKPNLDK 248
           L +KDLV LSGAHT+G SHCSS  +RL NF++  + DP+L+S +A  L+  KC   N D 
Sbjct: 183 LNLKDLVLLSGAHTIGVSHCSSMNTRLYNFSTTVKQDPSLDSQYAANLKANKCKSLN-DN 242

Query: 249 NAGQFLDPTSS-TFDNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAK-DQNLFFKEF 308
           +    +DP SS +FD  YY+ +++ +G+F SD AL  +S T +++       +  FFK F
Sbjct: 243 STILEMDPGSSRSFDLSYYRLVLKRRGLFQSDSALTTNSATLKVINDLVNGSEKKFFKAF 302

Query: 309 SASMVKLGNVGVSEN--GDVRVNCRV 329
           + SM K+G V V     G +R  C V
Sbjct: 303 AKSMEKMGRVKVKTGSAGVIRTRCSV 326

BLAST of MC10g0166 vs. ExPASy Swiss-Prot
Match: A7QEU4 (Peroxidase 5 OS=Vitis vinifera OX=29760 GN=GSVIVT00037159001 PE=1 SV=2)

HSP 1 Score: 272.7 bits (696), Expect = 5.4e-72
Identity = 149/315 (47.30%), Postives = 200/315 (63.49%), Query Frame = 0

Query: 24  LMLVVCVS---EGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLRLFFHDCFIR 83
           +++ +C+S   E  L   +Y  +C  AE I+   V    I D  V P L+R+ FHDCF+R
Sbjct: 14  VLVFLCLSVELEAQLQVGFYRTSCGLAEFIVKDEVRKGFIRDSGVAPGLVRMHFHDCFVR 73

Query: 84  GCDASVLIDSTPGKQAEKDAPP-NISLRSFYVIDEAKVKLETACPRTVSCADIVAIAARD 143
           GCD SVLIDSTP   AEKD+P  N SLR F VID AK +LE  C   VSCADIVA AARD
Sbjct: 74  GCDGSVLIDSTPSNTAEKDSPANNPSLRGFEVIDSAKARLEAVCKGVVSCADIVAFAARD 133

Query: 144 VVTLSGGPYWSVLKGRKDGKISNASE-TINLPAPTFNVSQLIQSFSNRGLQVKDLVALSG 203
            V ++GG  + V  GR+DG+IS ASE + NLP PTF V QL Q FSN+GL   ++V LSG
Sbjct: 134 SVEITGGLGYDVPAGRRDGRISLASEASTNLPPPTFTVDQLTQFFSNKGLTQDEMVTLSG 193

Query: 204 AHTLGFSHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQFLDPTSST 263
           AHT+G SHCSSF +RL NF      DPTL+  +A  L+ +CP+ + + N    ++P+S +
Sbjct: 194 AHTIGRSHCSSFSNRLYNFNGTSGQDPTLDPQYAASLKTQCPQGSTNTNLVVPMNPSSPS 253

Query: 264 FDNV-YYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKLGNVGV- 323
             +V YY  ++  +G+F SDQ L  D+ T   V   A +  L+  +F+++MVK+G +GV 
Sbjct: 254 ITDVGYYVDVLRNRGLFTSDQTLLTDTTTATQVRQNAGNPFLWKNKFASAMVKMGQLGVL 313

Query: 324 -SENGDVRVNCRVVN 331
             E G +R NCRV+N
Sbjct: 314 IGEAGQIRANCRVIN 328

BLAST of MC10g0166 vs. NCBI nr
Match: XP_022141163.1 (LOW QUALITY PROTEIN: peroxidase 66 [Momordica charantia])

HSP 1 Score: 621 bits (1602), Expect = 5.06e-224
Identity = 313/319 (98.12%), Postives = 313/319 (98.12%), Query Frame = 0

Query: 12  MATNARAVLFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLR 71
           MATNARAVLFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLR
Sbjct: 1   MATNARAVLFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLR 60

Query: 72  LFFHDCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCAD 131
           LFFHDCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCAD
Sbjct: 61  LFFHDCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCAD 120

Query: 132 IVAIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVK 191
           IVAIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVK
Sbjct: 121 IVAIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVK 180

Query: 192 DLVALSGAHTLGFSHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQF 251
           DLVALSG      SHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQF
Sbjct: 181 DLVALSGX-----SHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQF 240

Query: 252 LDPTSSTFDNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKL 311
           LDPTSSTFDNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKL
Sbjct: 241 LDPTSSTFDNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKL 300

Query: 312 GNVGVSENGDVRVNCRVVN 330
           GNVGVSENGDVRVNCRVVN
Sbjct: 301 GNVGVSENGDVRVNCRVVN 314

BLAST of MC10g0166 vs. NCBI nr
Match: XP_022945054.1 (peroxidase 66 [Cucurbita moschata])

HSP 1 Score: 578 bits (1489), Expect = 1.05e-206
Identity = 284/317 (89.59%), Postives = 298/317 (94.01%), Query Frame = 0

Query: 14  TNARAVLFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLRLF 73
           T A+    L LM++VCVSE VLD NYY+KTCPNAEKIILQTVYNASIHDPRVP RLLRLF
Sbjct: 4   TMAKNATVLFLMMIVCVSEAVLDTNYYSKTCPNAEKIILQTVYNASIHDPRVPARLLRLF 63

Query: 74  FHDCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCADIV 133
           FHDCFIRGCDASVLIDSTPG QAEKDAPPNISLRSFYVIDEAK KLETACP +VSCADIV
Sbjct: 64  FHDCFIRGCDASVLIDSTPGNQAEKDAPPNISLRSFYVIDEAKSKLETACPHSVSCADIV 123

Query: 134 AIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVKDL 193
           AIAARD+VTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSF+NRGLQVKDL
Sbjct: 124 AIAARDIVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFANRGLQVKDL 183

Query: 194 VALSGAHTLGFSHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQFLD 253
           VALSGAHTLGFSHCSSFQSRLRNF+SIH TDPTLESGFAQIL+ KCPKPNLDKNAGQFLD
Sbjct: 184 VALSGAHTLGFSHCSSFQSRLRNFSSIHDTDPTLESGFAQILKNKCPKPNLDKNAGQFLD 243

Query: 254 PTSSTFDNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKLGN 313
           PTSSTFDNVYYKRL+EGKGVFGSDQ+LFGDSRT+ IV SFA DQNLFFKEF+ASMVKLGN
Sbjct: 244 PTSSTFDNVYYKRLLEGKGVFGSDQSLFGDSRTKTIVESFANDQNLFFKEFAASMVKLGN 303

Query: 314 VGVSENGDVRVNCRVVN 330
           VGV ENG+VR NCRVVN
Sbjct: 304 VGVIENGNVRFNCRVVN 320

BLAST of MC10g0166 vs. NCBI nr
Match: KAG6574145.1 (Peroxidase 66, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013199.1 Peroxidase 66, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 574 bits (1480), Expect = 2.14e-205
Identity = 285/319 (89.34%), Postives = 298/319 (93.42%), Query Frame = 0

Query: 12  MATNARAVLFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLR 71
           MA NA     L LM++VCVSE VLD NYY+KTCPNAEKIILQTVYNASIHDP+VP RLLR
Sbjct: 1   MAKNATV---LFLMMIVCVSEAVLDTNYYSKTCPNAEKIILQTVYNASIHDPKVPARLLR 60

Query: 72  LFFHDCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCAD 131
           LFFHDCFIRGCDASVLIDS PG QAEKDAPPNISLRSFYVIDEAK KLETACP +VSCAD
Sbjct: 61  LFFHDCFIRGCDASVLIDSMPGNQAEKDAPPNISLRSFYVIDEAKSKLETACPHSVSCAD 120

Query: 132 IVAIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVK 191
           IVAIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSF+NRGLQVK
Sbjct: 121 IVAIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFANRGLQVK 180

Query: 192 DLVALSGAHTLGFSHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQF 251
           DLVALSGAHTLGFSHCSSFQSRLRNF+SIH TDPTLESGFAQIL+ KCPKPNLDKNAGQF
Sbjct: 181 DLVALSGAHTLGFSHCSSFQSRLRNFSSIHDTDPTLESGFAQILKNKCPKPNLDKNAGQF 240

Query: 252 LDPTSSTFDNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKL 311
           LDPTSSTFDNVYYKRL+EGKGVFGSDQ+LFGDSRT+ IV SFA DQNLFFKEF+ASMVKL
Sbjct: 241 LDPTSSTFDNVYYKRLLEGKGVFGSDQSLFGDSRTKTIVESFANDQNLFFKEFAASMVKL 300

Query: 312 GNVGVSENGDVRVNCRVVN 330
           GNVGV ENG+VR NCRVVN
Sbjct: 301 GNVGVIENGNVRFNCRVVN 316

BLAST of MC10g0166 vs. NCBI nr
Match: XP_022968458.1 (peroxidase 66 [Cucurbita maxima])

HSP 1 Score: 573 bits (1476), Expect = 1.01e-204
Identity = 281/317 (88.64%), Postives = 296/317 (93.38%), Query Frame = 0

Query: 14  TNARAVLFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLRLF 73
           T A+    L LM++VCVSE VLD NYY+KTCPNAEKIILQTVYNASIHDP+VP RLLRLF
Sbjct: 4   TMAKNATVLFLMMIVCVSEAVLDTNYYSKTCPNAEKIILQTVYNASIHDPKVPARLLRLF 63

Query: 74  FHDCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCADIV 133
           FHDCFIRGCDASVLIDS PG QAEKDAPPNISLRSFYVIDEAK KLETACP +VSCADIV
Sbjct: 64  FHDCFIRGCDASVLIDSAPGNQAEKDAPPNISLRSFYVIDEAKSKLETACPHSVSCADIV 123

Query: 134 AIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVKDL 193
           AIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSF+NRGLQVKDL
Sbjct: 124 AIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFANRGLQVKDL 183

Query: 194 VALSGAHTLGFSHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQFLD 253
           VALSGAHTLGFSHCSSFQSRLRNF+S+H TDPTLESGF QIL+ KCPKPNLDKNAGQFLD
Sbjct: 184 VALSGAHTLGFSHCSSFQSRLRNFSSLHDTDPTLESGFTQILKNKCPKPNLDKNAGQFLD 243

Query: 254 PTSSTFDNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKLGN 313
           PTSSTFDNVYYKRL+EGKGVFGSDQ+LFGDSRT+ IV SFA DQNLFFKEF+ASMVKLGN
Sbjct: 244 PTSSTFDNVYYKRLLEGKGVFGSDQSLFGDSRTKTIVESFANDQNLFFKEFAASMVKLGN 303

Query: 314 VGVSENGDVRVNCRVVN 330
           VGV ENG+VR NCRVVN
Sbjct: 304 VGVIENGNVRFNCRVVN 320

BLAST of MC10g0166 vs. NCBI nr
Match: XP_023541471.1 (peroxidase 66 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 571 bits (1471), Expect = 5.02e-204
Identity = 283/319 (88.71%), Postives = 297/319 (93.10%), Query Frame = 0

Query: 12  MATNARAVLFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLR 71
           MA NA     L LM++VCVSE VL  NYY+KTCPNAEKIILQTVYNASIHDP+VP RLLR
Sbjct: 1   MAKNATV---LFLMMIVCVSEAVLHTNYYSKTCPNAEKIILQTVYNASIHDPKVPARLLR 60

Query: 72  LFFHDCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCAD 131
           LFFHDCFIRGCDASVLIDSTPG QAEKDAPPNISLRSFYVIDEAK KLETACP +VSCAD
Sbjct: 61  LFFHDCFIRGCDASVLIDSTPGNQAEKDAPPNISLRSFYVIDEAKSKLETACPHSVSCAD 120

Query: 132 IVAIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVK 191
           IVAIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSF NRGLQVK
Sbjct: 121 IVAIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFENRGLQVK 180

Query: 192 DLVALSGAHTLGFSHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQF 251
           DLVALSGAHTLGFSHCSSFQSRLRNF++IH TDPTLESGFAQIL+ KCPKPNLDKNAGQF
Sbjct: 181 DLVALSGAHTLGFSHCSSFQSRLRNFSTIHDTDPTLESGFAQILKNKCPKPNLDKNAGQF 240

Query: 252 LDPTSSTFDNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKL 311
           LDPTSSTFDNVYYKRL+EGKGVFGSDQ+LFGDSRT+ IV SFA +QNLFFKEF+ASMVKL
Sbjct: 241 LDPTSSTFDNVYYKRLLEGKGVFGSDQSLFGDSRTKTIVESFANEQNLFFKEFAASMVKL 300

Query: 312 GNVGVSENGDVRVNCRVVN 330
           GNVGV ENG+VR NCRVVN
Sbjct: 301 GNVGVIENGNVRFNCRVVN 316

BLAST of MC10g0166 vs. ExPASy TrEMBL
Match: A0A6J1CJP1 (Peroxidase OS=Momordica charantia OX=3673 GN=LOC111011620 PE=3 SV=1)

HSP 1 Score: 621 bits (1602), Expect = 2.45e-224
Identity = 313/319 (98.12%), Postives = 313/319 (98.12%), Query Frame = 0

Query: 12  MATNARAVLFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLR 71
           MATNARAVLFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLR
Sbjct: 1   MATNARAVLFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLR 60

Query: 72  LFFHDCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCAD 131
           LFFHDCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCAD
Sbjct: 61  LFFHDCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCAD 120

Query: 132 IVAIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVK 191
           IVAIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVK
Sbjct: 121 IVAIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVK 180

Query: 192 DLVALSGAHTLGFSHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQF 251
           DLVALSG      SHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQF
Sbjct: 181 DLVALSGX-----SHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQF 240

Query: 252 LDPTSSTFDNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKL 311
           LDPTSSTFDNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKL
Sbjct: 241 LDPTSSTFDNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKL 300

Query: 312 GNVGVSENGDVRVNCRVVN 330
           GNVGVSENGDVRVNCRVVN
Sbjct: 301 GNVGVSENGDVRVNCRVVN 314

BLAST of MC10g0166 vs. ExPASy TrEMBL
Match: A0A6J1FZW5 (Peroxidase OS=Cucurbita moschata OX=3662 GN=LOC111449412 PE=3 SV=1)

HSP 1 Score: 578 bits (1489), Expect = 5.10e-207
Identity = 284/317 (89.59%), Postives = 298/317 (94.01%), Query Frame = 0

Query: 14  TNARAVLFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLRLF 73
           T A+    L LM++VCVSE VLD NYY+KTCPNAEKIILQTVYNASIHDPRVP RLLRLF
Sbjct: 4   TMAKNATVLFLMMIVCVSEAVLDTNYYSKTCPNAEKIILQTVYNASIHDPRVPARLLRLF 63

Query: 74  FHDCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCADIV 133
           FHDCFIRGCDASVLIDSTPG QAEKDAPPNISLRSFYVIDEAK KLETACP +VSCADIV
Sbjct: 64  FHDCFIRGCDASVLIDSTPGNQAEKDAPPNISLRSFYVIDEAKSKLETACPHSVSCADIV 123

Query: 134 AIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVKDL 193
           AIAARD+VTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSF+NRGLQVKDL
Sbjct: 124 AIAARDIVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFANRGLQVKDL 183

Query: 194 VALSGAHTLGFSHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQFLD 253
           VALSGAHTLGFSHCSSFQSRLRNF+SIH TDPTLESGFAQIL+ KCPKPNLDKNAGQFLD
Sbjct: 184 VALSGAHTLGFSHCSSFQSRLRNFSSIHDTDPTLESGFAQILKNKCPKPNLDKNAGQFLD 243

Query: 254 PTSSTFDNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKLGN 313
           PTSSTFDNVYYKRL+EGKGVFGSDQ+LFGDSRT+ IV SFA DQNLFFKEF+ASMVKLGN
Sbjct: 244 PTSSTFDNVYYKRLLEGKGVFGSDQSLFGDSRTKTIVESFANDQNLFFKEFAASMVKLGN 303

Query: 314 VGVSENGDVRVNCRVVN 330
           VGV ENG+VR NCRVVN
Sbjct: 304 VGVIENGNVRFNCRVVN 320

BLAST of MC10g0166 vs. ExPASy TrEMBL
Match: A0A6J1HY26 (Peroxidase OS=Cucurbita maxima OX=3661 GN=LOC111467696 PE=3 SV=1)

HSP 1 Score: 573 bits (1476), Expect = 4.88e-205
Identity = 281/317 (88.64%), Postives = 296/317 (93.38%), Query Frame = 0

Query: 14  TNARAVLFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLRLF 73
           T A+    L LM++VCVSE VLD NYY+KTCPNAEKIILQTVYNASIHDP+VP RLLRLF
Sbjct: 4   TMAKNATVLFLMMIVCVSEAVLDTNYYSKTCPNAEKIILQTVYNASIHDPKVPARLLRLF 63

Query: 74  FHDCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCADIV 133
           FHDCFIRGCDASVLIDS PG QAEKDAPPNISLRSFYVIDEAK KLETACP +VSCADIV
Sbjct: 64  FHDCFIRGCDASVLIDSAPGNQAEKDAPPNISLRSFYVIDEAKSKLETACPHSVSCADIV 123

Query: 134 AIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVKDL 193
           AIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSF+NRGLQVKDL
Sbjct: 124 AIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFANRGLQVKDL 183

Query: 194 VALSGAHTLGFSHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQFLD 253
           VALSGAHTLGFSHCSSFQSRLRNF+S+H TDPTLESGF QIL+ KCPKPNLDKNAGQFLD
Sbjct: 184 VALSGAHTLGFSHCSSFQSRLRNFSSLHDTDPTLESGFTQILKNKCPKPNLDKNAGQFLD 243

Query: 254 PTSSTFDNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKLGN 313
           PTSSTFDNVYYKRL+EGKGVFGSDQ+LFGDSRT+ IV SFA DQNLFFKEF+ASMVKLGN
Sbjct: 244 PTSSTFDNVYYKRLLEGKGVFGSDQSLFGDSRTKTIVESFANDQNLFFKEFAASMVKLGN 303

Query: 314 VGVSENGDVRVNCRVVN 330
           VGV ENG+VR NCRVVN
Sbjct: 304 VGVIENGNVRFNCRVVN 320

BLAST of MC10g0166 vs. ExPASy TrEMBL
Match: A0A1S3AYQ9 (Peroxidase OS=Cucumis melo OX=3656 GN=LOC103484034 PE=3 SV=1)

HSP 1 Score: 553 bits (1426), Expect = 1.74e-197
Identity = 273/319 (85.58%), Postives = 295/319 (92.48%), Query Frame = 0

Query: 12  MATNARAVLFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLR 71
           MA NA ++LFL++M  VC+SEGVLD +YY KTCPNAE IILQTVYNASIHDP+VP RLLR
Sbjct: 1   MAKNA-SLLFLIMM--VCLSEGVLDSHYYLKTCPNAENIILQTVYNASIHDPKVPARLLR 60

Query: 72  LFFHDCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCAD 131
           LFFHDCFIRGCD SVLIDSTP  QAEKD PPNISLRSFYVIDEAK KLE+ACP TVSCAD
Sbjct: 61  LFFHDCFIRGCDGSVLIDSTPENQAEKDGPPNISLRSFYVIDEAKAKLESACPHTVSCAD 120

Query: 132 IVAIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVK 191
           IVAIAARDVVTLSGGPYWSVLKGRKDGKIS ASETINLPAPTFNVSQLIQSF++RGL+VK
Sbjct: 121 IVAIAARDVVTLSGGPYWSVLKGRKDGKISKASETINLPAPTFNVSQLIQSFADRGLEVK 180

Query: 192 DLVALSGAHTLGFSHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQF 251
           D+VALSGAHTLGFSHCSSFQSRLRNF+S H+ DPTLESGFAQILR KCPKPNLDKNAGQF
Sbjct: 181 DMVALSGAHTLGFSHCSSFQSRLRNFSSTHEIDPTLESGFAQILRNKCPKPNLDKNAGQF 240

Query: 252 LDPTSSTFDNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKL 311
           LDPTSSTFDNVYYKRL+EGKGVFGSDQALF DSRTR +VG FA+DQNLFFKEF+ASMV L
Sbjct: 241 LDPTSSTFDNVYYKRLLEGKGVFGSDQALFVDSRTRGLVGQFAQDQNLFFKEFAASMVNL 300

Query: 312 GNVGVSENGDVRVNCRVVN 330
           GNVGV +NG++R+NCRV N
Sbjct: 301 GNVGVIQNGNIRINCRVPN 316

BLAST of MC10g0166 vs. ExPASy TrEMBL
Match: A0A0A0L6C8 (Peroxidase OS=Cucumis sativus OX=3659 GN=Csa_3G179180 PE=3 SV=1)

HSP 1 Score: 550 bits (1417), Expect = 4.10e-196
Identity = 267/315 (84.76%), Postives = 291/315 (92.38%), Query Frame = 0

Query: 16  ARAVLFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLRLFFH 75
           A++   L L+++VC+SE VLD +YY+KTCPNAE IILQTVYNASIHDP+VP RLLRLFFH
Sbjct: 2   AKSASLLFLIMMVCLSEAVLDSHYYSKTCPNAENIILQTVYNASIHDPKVPARLLRLFFH 61

Query: 76  DCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCADIVAI 135
           DCFIRGCD SVLIDSTP  QAEKDAPPNISLRSFYVIDEAK KLE+ACP TVSCADIVAI
Sbjct: 62  DCFIRGCDGSVLIDSTPENQAEKDAPPNISLRSFYVIDEAKAKLESACPHTVSCADIVAI 121

Query: 136 AARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVKDLVA 195
           AARDVVTLSGGPYWSVLKGRKDGKIS ASETINLPAPTFNVSQLIQSF+NRGL VKD+VA
Sbjct: 122 AARDVVTLSGGPYWSVLKGRKDGKISKASETINLPAPTFNVSQLIQSFANRGLDVKDMVA 181

Query: 196 LSGAHTLGFSHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQFLDPT 255
           LSGAHTLGFSHCSSFQSRLRNF++ H+ DPTLESGFAQILR KCPKPN+DKNAGQFLDPT
Sbjct: 182 LSGAHTLGFSHCSSFQSRLRNFSATHEIDPTLESGFAQILRNKCPKPNVDKNAGQFLDPT 241

Query: 256 SSTFDNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKLGNVG 315
           SSTFDNVYYKRL+EGKGVFGSDQALF DSRTR +VG FA+DQNLFFKEF+ASMV LGNVG
Sbjct: 242 SSTFDNVYYKRLLEGKGVFGSDQALFVDSRTRGLVGLFAQDQNLFFKEFAASMVSLGNVG 301

Query: 316 VSENGDVRVNCRVVN 330
           V +NG+VR++CRV N
Sbjct: 302 VIQNGNVRIDCRVPN 316

BLAST of MC10g0166 vs. TAIR 10
Match: AT5G51890.1 (Peroxidase superfamily protein )

HSP 1 Score: 455.7 bits (1171), Expect = 3.2e-128
Identity = 219/319 (68.65%), Postives = 262/319 (82.13%), Query Frame = 0

Query: 16  ARAVLFLMLMLVVCV---SEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLRL 75
           ++ ++F M+  V+ +   SE  LD +YY ++CP AEKIIL+TV NA+++DP+VP RLLR+
Sbjct: 4   SKGLIFAMIFAVLAIVKPSEAALDAHYYDQSCPAAEKIILETVRNATLYDPKVPARLLRM 63

Query: 76  FFHDCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCADI 135
           FFHDCFIRGCDAS+L+DST   QAEKD PPNIS+RSFYVI++AK KLE ACPRTVSCAD+
Sbjct: 64  FFHDCFIRGCDASILLDSTRSNQAEKDGPPNISVRSFYVIEDAKRKLEKACPRTVSCADV 123

Query: 136 VAIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVKD 195
           +AIAARDVVTLSGGPYWSVLKGRKDG IS A+ET NLP PTFNVSQLIQSF+ RGL VKD
Sbjct: 124 IAIAARDVVTLSGGPYWSVLKGRKDGTISRANETRNLPPPTFNVSQLIQSFAARGLSVKD 183

Query: 196 LVALSGAHTLGFSHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPK-PNLDKNAGQF 255
           +V LSG HT+GFSHCSSF+SRL+NF+  H  DP++   FAQ L++KCP+  N  KNAG  
Sbjct: 184 MVTLSGGHTIGFSHCSSFESRLQNFSKFHDIDPSMNYAFAQTLKKKCPRTSNRGKNAGTV 243

Query: 256 LDPTSSTFDNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKL 315
           LD TSS FDNVYYK+++ GKGVFGSDQAL GDSRT+ IV +FA+DQ  FF+EF+ASMVKL
Sbjct: 244 LDSTSSVFDNVYYKQILSGKGVFGSDQALLGDSRTKWIVETFAQDQKAFFREFAASMVKL 303

Query: 316 GNVGVSENGDVRVNCRVVN 331
           GN GV E G VRVN R VN
Sbjct: 304 GNFGVKETGQVRVNTRFVN 322

BLAST of MC10g0166 vs. TAIR 10
Match: AT5G42180.1 (Peroxidase superfamily protein )

HSP 1 Score: 332.4 bits (851), Expect = 4.1e-91
Identity = 163/310 (52.58%), Postives = 209/310 (67.42%), Query Frame = 0

Query: 20  LFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLRLFFHDCFI 79
           L ++++ VV      L  +YY  TCP A+ I+   V  A  +D  VP  LLR+ FHDCF+
Sbjct: 8   LLVIVIFVVSFDVQALSPHYYDHTCPQADHIVTNAVKKAMSNDQTVPAALLRMHFHDCFV 67

Query: 80  RGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCADIVAIAARD 139
           RGCD SVL+DS    +AEKD PPNISL +FYVID AK  LE  CP  VSCADI+++AARD
Sbjct: 68  RGCDGSVLLDSKGKNKAEKDGPPNISLHAFYVIDNAKKALEEQCPGIVSCADILSLAARD 127

Query: 140 VVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVKDLVALSGA 199
            V LSGGP W+V KGRKDG+IS A ET  LPAPTFN+SQL Q+F  RGL + DLVALSG 
Sbjct: 128 AVALSGGPTWAVPKGRKDGRISKAIETRQLPAPTFNISQLRQNFGQRGLSMHDLVALSGG 187

Query: 200 HTLGFSHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQFLDPTSSTF 259
           HTLGF+HCSSFQ+RL  F +  + DPTL   FA  L   CP  N  KNAG  +D T ++F
Sbjct: 188 HTLGFAHCSSFQNRLHKFNTQKEVDPTLNPSFAARLEGVCPAHNTVKNAGSNMDGTVTSF 247

Query: 260 DNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKLGNVGVSEN 319
           DN+YYK L++GK +F SD++L     T+++V  +A     F + F  SM+K+ ++  + N
Sbjct: 248 DNIYYKMLIQGKSLFSSDESLLAVPSTKKLVAKYANSNEEFERAFVKSMIKMSSISGNGN 307

Query: 320 GDVRVNCRVV 330
            +VR+NCR V
Sbjct: 308 -EVRLNCRRV 316

BLAST of MC10g0166 vs. TAIR 10
Match: AT4G33420.1 (Peroxidase superfamily protein )

HSP 1 Score: 281.2 bits (718), Expect = 1.1e-75
Identity = 146/298 (48.99%), Postives = 188/298 (63.09%), Query Frame = 0

Query: 35  LDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPRLLRLFFHDCFIRGCDASVLIDSTPGK 94
           L   YY  +CP AE+I+  +V NA   DP +   L+R+ FHDCFI GCDAS+L+DST   
Sbjct: 37  LSMTYYMMSCPFAEQIVKNSVNNALQADPTLAAGLIRMLFHDCFIEGCDASILLDSTKDN 96

Query: 95  QAEKDAPPNISLRSFYVIDEAKVKLETACPRTVSCADIVAIAARDVVTLSGGPYWSVLKG 154
            AEKD+P N+SLR + +ID+AK K+E  CP  VSCADIVA+AARD V  +GGPY+ + KG
Sbjct: 97  TAEKDSPANLSLRGYEIIDDAKEKIENRCPGVVSCADIVAMAARDAVFWAGGPYYDIPKG 156

Query: 155 RKDGKISNASETINLPAPTFNVSQLIQSFSNRGLQVKDLVALSGAHTLGFSHCSSFQSRL 214
           R DGK S   +T NLP+P  N SQLIQ+F  RG   +D+VALSGAHTLG + CSSF++RL
Sbjct: 157 RFDGKRSKIEDTRNLPSPFLNASQLIQTFGQRGFTPQDVVALSGAHTLGVARCSSFKARL 216

Query: 215 RNFTSIHQTDPTLESGFAQILREKCPKPNLDKNAGQFLDPTSSTFDNVYYKRLMEGKGVF 274
                    D +L+S FA  L + C   +   NA Q  D T + FDN Y+  L    GV 
Sbjct: 217 T------VPDSSLDSTFANTLSKTC---SAGDNAEQPFDATRNDFDNAYFNALQMKSGVL 276

Query: 275 GSDQALFGDSRTRRIVGSFAKDQNLFFKEFSASMVKLGNVGV--SENGDVRVNCRVVN 331
            SDQ LF   RTR +V  +A +Q  FF +F  +M K+ N+ V     G+VR NCR +N
Sbjct: 277 FSDQTLFNTPRTRNLVNGYALNQAKFFFDFQQAMRKMSNLDVKLGSQGEVRQNCRSIN 325

BLAST of MC10g0166 vs. TAIR 10
Match: AT3G21770.1 (Peroxidase superfamily protein )

HSP 1 Score: 275.0 bits (702), Expect = 7.8e-74
Identity = 150/326 (46.01%), Postives = 208/326 (63.80%), Query Frame = 0

Query: 9   TIQMATNARAVLFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPR 68
           T+     A  V+  +L+ ++  SE  L  N+Y K+CPNAEKII   + N   + P +   
Sbjct: 3   TMTQLNIAVVVVVTVLIGMLRSSEAQLQMNFYAKSCPNAEKIISDHIQNHIHNGPSLAAP 62

Query: 69  LLRLFFHDCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVS 128
           L+R+ FHDCF+RGCD SVLI+ST G  AE+DAPPN++LR F  ++  K  LE  CP+TVS
Sbjct: 63  LIRMHFHDCFVRGCDGSVLINSTSG-NAERDAPPNLTLRGFGFVERIKALLEKVCPKTVS 122

Query: 129 CADIVAIAARDVVTLSGGPYWSVLKGRKDGKISNASE-TINLPAPTFNVSQLIQSFSNRG 188
           CADI+A+ ARD V  +GGP WSV  GR+DG+ISN +E T N+P PT N + L + F N+G
Sbjct: 123 CADIIALTARDAVVATGGPSWSVPTGRRDGRISNKTEATNNIPPPTSNFTTLQRLFKNQG 182

Query: 189 LQVKDLVALSGAHTLGFSHCSSFQSRLRNFTSIHQTDPTLESGFAQILR-EKCPKPNLDK 248
           L +KDLV LSGAHT+G SHCSS  +RL NF++  + DP+L+S +A  L+  KC   N D 
Sbjct: 183 LNLKDLVLLSGAHTIGVSHCSSMNTRLYNFSTTVKQDPSLDSQYAANLKANKCKSLN-DN 242

Query: 249 NAGQFLDPTSS-TFDNVYYKRLMEGKGVFGSDQALFGDSRTRRIVGSFAK-DQNLFFKEF 308
           +    +DP SS +FD  YY+ +++ +G+F SD AL  +S T +++       +  FFK F
Sbjct: 243 STILEMDPGSSRSFDLSYYRLVLKRRGLFQSDSALTTNSATLKVINDLVNGSEKKFFKAF 302

Query: 309 SASMVKLGNVGVSEN--GDVRVNCRV 329
           + SM K+G V V     G +R  C V
Sbjct: 303 AKSMEKMGRVKVKTGSAGVIRTRCSV 326

BLAST of MC10g0166 vs. TAIR 10
Match: AT3G01190.1 (Peroxidase superfamily protein )

HSP 1 Score: 269.2 bits (687), Expect = 4.3e-72
Identity = 146/326 (44.79%), Postives = 204/326 (62.58%), Query Frame = 0

Query: 12  MATNARAV---LFLMLMLVVCVSEGVLDGNYYTKTCPNAEKIILQTVYNASIHDPRVPPR 71
           MA + R V   LFL+L+     S+G+  G +Y+KTCP  E I+ + V++A    P +   
Sbjct: 1   MAASKRLVVSCLFLVLLFAQANSQGLKVG-FYSKTCPQLEGIVKKVVFDAMNKAPTLGAP 60

Query: 72  LLRLFFHDCFIRGCDASVLIDSTPGKQAEKDAPPNISLRSFYVIDEAKVKLETACPRTVS 131
           LLR+FFHDCF+RGCD SVL+D  P  Q EK A PN+SLR F +ID++K  LE  CP  VS
Sbjct: 61  LLRMFFHDCFVRGCDGSVLLDK-PNNQGEKSAVPNLSLRGFGIIDDSKAALEKVCPGIVS 120

Query: 132 CADIVAIAARDVVTLSGGPYWSVLKGRKDGKISNASETINLPAPTFNVSQLIQSFSNRGL 191
           C+DI+A+ ARD +    GP W V  GR+DG++SN +E +NLP+P  N+++LI  F ++GL
Sbjct: 121 CSDILALVARDAMVALEGPSWEVETGRRDGRVSNINE-VNLPSPFDNITKLISDFRSKGL 180

Query: 192 QVKDLVALSGAHTLGFSHCSSFQSRLRNFTSIHQTDPTLESGFAQILREKCPKPNLDKNA 251
             KDLV LSG HT+G  HC    +RL NFT    +DP+L+S +A  LR+KC KP  D   
Sbjct: 181 NEKDLVILSGGHTIGMGHCPLLTNRLYNFTGKGDSDPSLDSEYAAKLRKKC-KPT-DTTT 240

Query: 252 GQFLDPTS-STFDNVYYKRLMEGKGVFGSDQALFGDSRTRR-IVGSFAKDQNLFFKEFSA 311
              +DP S  TFD  Y+  + + +G+F SD AL  +S+TR  ++       ++FF +F  
Sbjct: 241 ALEMDPGSFKTFDLSYFTLVAKRRGLFQSDAALLDNSKTRAYVLQQIRTHGSMFFNDFGV 300

Query: 312 SMVKLGNVGV--SENGDVRVNCRVVN 331
           SMVK+G  GV   + G++R  CR  N
Sbjct: 301 SMVKMGRTGVLTGKAGEIRKTCRSAN 321

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LT914.5e-12768.65Peroxidase 66 OS=Arabidopsis thaliana OX=3702 GN=PER66 PE=2 SV=1[more]
Q438725.8e-9052.58Peroxidase 64 OS=Arabidopsis thaliana OX=3702 GN=PER64 PE=1 SV=1[more]
Q9SZB91.5e-7448.99Peroxidase 47 OS=Arabidopsis thaliana OX=3702 GN=PER47 PE=2 SV=2[more]
Q9LSY71.1e-7246.01Peroxidase 30 OS=Arabidopsis thaliana OX=3702 GN=PER30 PE=1 SV=1[more]
A7QEU45.4e-7247.30Peroxidase 5 OS=Vitis vinifera OX=29760 GN=GSVIVT00037159001 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_022141163.15.06e-22498.12LOW QUALITY PROTEIN: peroxidase 66 [Momordica charantia][more]
XP_022945054.11.05e-20689.59peroxidase 66 [Cucurbita moschata][more]
KAG6574145.12.14e-20589.34Peroxidase 66, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013199.1 Per... [more]
XP_022968458.11.01e-20488.64peroxidase 66 [Cucurbita maxima][more]
XP_023541471.15.02e-20488.71peroxidase 66 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1CJP12.45e-22498.12Peroxidase OS=Momordica charantia OX=3673 GN=LOC111011620 PE=3 SV=1[more]
A0A6J1FZW55.10e-20789.59Peroxidase OS=Cucurbita moschata OX=3662 GN=LOC111449412 PE=3 SV=1[more]
A0A6J1HY264.88e-20588.64Peroxidase OS=Cucurbita maxima OX=3661 GN=LOC111467696 PE=3 SV=1[more]
A0A1S3AYQ91.74e-19785.58Peroxidase OS=Cucumis melo OX=3656 GN=LOC103484034 PE=3 SV=1[more]
A0A0A0L6C84.10e-19684.76Peroxidase OS=Cucumis sativus OX=3659 GN=Csa_3G179180 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G51890.13.2e-12868.65Peroxidase superfamily protein [more]
AT5G42180.14.1e-9152.58Peroxidase superfamily protein [more]
AT4G33420.11.1e-7548.99Peroxidase superfamily protein [more]
AT3G21770.17.8e-7446.01Peroxidase superfamily protein [more]
AT3G01190.14.3e-7244.79Peroxidase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002016Haem peroxidasePRINTSPR00458PEROXIDASEcoord: 145..157
score: 49.33
coord: 127..144
score: 44.79
coord: 192..207
score: 57.84
coord: 252..267
score: 34.54
coord: 66..80
score: 43.95
IPR002016Haem peroxidasePFAMPF00141peroxidasecoord: 57..296
e-value: 7.4E-75
score: 251.6
IPR002016Haem peroxidasePROSITEPS50873PEROXIDASE_4coord: 34..330
score: 77.385193
IPR000823Plant peroxidasePRINTSPR00461PLPEROXIDASEcoord: 306..319
score: 35.92
coord: 44..63
score: 34.31
coord: 107..120
score: 44.68
coord: 68..88
score: 67.6
coord: 191..203
score: 74.96
coord: 266..283
score: 43.14
coord: 126..136
score: 74.15
coord: 250..265
score: 35.72
coord: 145..160
score: 52.33
NoneNo IPR availableGENE3D1.10.520.10coord: 38..330
e-value: 5.3E-117
score: 392.3
NoneNo IPR availableGENE3D1.10.420.10Peroxidase, domain 2coord: 170..311
e-value: 5.3E-117
score: 392.3
NoneNo IPR availablePIRSRPIRSR600823-4PIRSR600823-4coord: 17..330
e-value: 1.4E-117
score: 390.2
NoneNo IPR availablePANTHERPTHR31235PEROXIDASE 25-RELATEDcoord: 18..329
NoneNo IPR availablePANTHERPTHR31235:SF247PEROXIDASE 66coord: 18..329
IPR019793Peroxidases heam-ligand binding sitePROSITEPS00435PEROXIDASE_1coord: 192..202
IPR033905Secretory peroxidaseCDDcd00693secretory_peroxidasecoord: 35..329
e-value: 2.08006E-177
score: 490.873
IPR010255Haem peroxidase superfamilySUPERFAMILY48113Heme-dependent peroxidasescoord: 35..330

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g0166.1MC10g0166.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098869 cellular oxidant detoxification
biological_process GO:0042744 hydrogen peroxide catabolic process
biological_process GO:0006979 response to oxidative stress
cellular_component GO:0005576 extracellular region
cellular_component GO:0009505 plant-type cell wall
molecular_function GO:0020037 heme binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004601 peroxidase activity