MC09g_new0496 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g_new0496
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionP-loop containing nucleoside triphosphate hydrolases superfamily protein
LocationMC09: 23500996 .. 23502637 (+)
RNA-Seq ExpressionMC09g_new0496
SyntenyMC09g_new0496
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGTGATGGGGGAGATCAGAACAATTATCCACCAAATTCTTCCCCAACAACTCCGCCATGTAACCTCCTCCAAATTCAACGCCATTTTTGACTTCGGGATTCTCTCTTCTCAAAAGTTGGTTTACATAATCGGCGAGAGCAACGGAGTCACCACCAACGAGCTCTACAGAGCCACCGAAACGTACCTCCACACAAAAATTCCGCTATCTGTGAAGCAGCTCGAAGCTTCCAAAGCCAAAGAGCAGAAGAATTTCTCCTTCAAAATCAGCAAAGGCGAGAGTTTAACCGACGAATTCCAAGGGATTCAAATCACCTGGGAATTGCATTCCATCCAAAAGGATCAGAAACCCAATTCTGAAAAACGGTACTACCAAATGAGCTTCCTCAAAAAACATAAAGATTTTGTCGACGATGTATACCTTCCGTACATCATGAACAGAGCAAACGCCATAGACGAAGGAAACAGAGTGGTGAAACTTTACTCTTTGGGGTATGATGAATCCTCCATTGTTTTGCGGAATACGTGCTCGTTTGAGACATTGGCTATGGATCCGGAGAAGAAGAAGGAGGTGATGGATGATCTGGACAGATTTGTGAGGAGGAAGGATTTTTACAGGAGGCTTGGGAGGGCCTGGAAAAGAGGGTATATTTTGTATGGCCCACCGGGAACTGGGAAATCCAGCTTGGTGCTGGCCATGGCTAATTATCTCAAGTTTAGTATCTACGATTTGGAGCTCACGAGCGTTGACAGTAACTCAGAGTTCAGGGAGATGATGCTGTGCACTGCTGACCGATCAATTATTGTCATTGAAGATATTGATTGCAGCACTGAGCTTGAGGATCGTGAATGTGACGATTATGGCGACCGCAAAGGAAAGGCGAGTTAACTTATCGATTTAAGCTCTTGGGTTGAGGGCTAGTTTACTTATTGTCGGCTACGTGAATTAATTTTTTATCTTTCTGTATTTGCAGCTGACTTTGTCTGGAGTTCTAAACGCCATTGATGGGCTATGGTCGAGCTGTGGGGATGCGAGGATTATAGTTTTCACGACGAATCATAAGGAGAAGTTGGACCCAGCACTGTTAAGGCCAGGGCGAATGGATATGCACATCCACATGACTTATTTGACCCCATCTGGATTTCAGATTCTGGCCTCAAACTATCTGCAGATTAAAAGCCATGATCGATTCAAAGAAATCGAAGAGCTGATAATGGAGGTGGAGGTCACGCCGGCGGAAATTGCAGAGGAGCTCATGAAAAGCGACGATGCTGACGTGGCCCTTGGAGCAGTGGTGGAATTCATCAATGGCAAGAAGAGGAAGAGGGTGGAAAAGGACGAACGGGACCCCCAAGAAGACATTGAGGAAGAAGACCCGAAGGAGTTCAAACAAAAAAAGGGAAACACAAGAAGAAGAAGGCACATGAGATGGAGACGGGATCACTCTGATGAGTGGTGATCAGTCAATAAACTAGTTTTTATGGAAAATATTTAGGTCCTTAGATCAAATTTCTAATACTTTTTGCTTAATTTTTTCATCGTAATTTCTTTGAATTAAGTGTCATGTTATACATTGGTGAGCATAATATCTCCATGAAATTCTTATTTTGGTCCCAATTTTTCAAACCTAATTTCAATTTTAC

mRNA sequence

ATGGGGGAGATCAGAACAATTATCCACCAAATTCTTCCCCAACAACTCCGCCATGTAACCTCCTCCAAATTCAACGCCATTTTTGACTTCGGGATTCTCTCTTCTCAAAAGTTGGTTTACATAATCGGCGAGAGCAACGGAGTCACCACCAACGAGCTCTACAGAGCCACCGAAACGTACCTCCACACAAAAATTCCGCTATCTGTGAAGCAGCTCGAAGCTTCCAAAGCCAAAGAGCAGAAGAATTTCTCCTTCAAAATCAGCAAAGGCGAGAGTTTAACCGACGAATTCCAAGGGATTCAAATCACCTGGGAATTGCATTCCATCCAAAAGGATCAGAAACCCAATTCTGAAAAACGGTACTACCAAATGAGCTTCCTCAAAAAACATAAAGATTTTGTCGACGATGTATACCTTCCGTACATCATGAACAGAGCAAACGCCATAGACGAAGGAAACAGAGTGGTGAAACTTTACTCTTTGGGGTATGATGAATCCTCCATTGTTTTGCGGAATACGTGCTCGTTTGAGACATTGGCTATGGATCCGGAGAAGAAGAAGGAGGTGATGGATGATCTGGACAGATTTGTGAGGAGGAAGGATTTTTACAGGAGGCTTGGGAGGGCCTGGAAAAGAGGGTATATTTTGTATGGCCCACCGGGAACTGGGAAATCCAGCTTGGTGCTGGCCATGGCTAATTATCTCAAGTTTAGTATCTACGATTTGGAGCTCACGAGCGTTGACAGTAACTCAGAGTTCAGGGAGATGATGCTGTGCACTGCTGACCGATCAATTATTGTCATTGAAGATATTGATTGCAGCACTGAGCTTGAGGATCGTGAATGTGACGATTATGGCGACCGCAAAGGAAAGCTGACTTTGTCTGGAGTTCTAAACGCCATTGATGGGCTATGGTCGAGCTGTGGGGATGCGAGGATTATAGTTTTCACGACGAATCATAAGGAGAAGTTGGACCCAGCACTGTTAAGGCCAGGGCGAATGGATATGCACATCCACATGACTTATTTGACCCCATCTGGATTTCAGATTCTGGCCTCAAACTATCTGCAGATTAAAAGCCATGATCGATTCAAAGAAATCGAAGAGCTGATAATGGAGGTGGAGGTCACGCCGGCGGAAATTGCAGAGGAGCTCATGAAAAGCGACGATGCTGACGTGGCCCTTGGAGCAGTGGTGGAATTCATCAATGGCAAGAAGAGGAAGAGGGTGGAAAAGGACGAACGGGACCCCCAAGAAGACATTGAGGAAGAAGACCCGAAGGAGTTCAAACAAAAAAAGGGAAACACAAGAAGAAGAAGGCACATGAGATGGAGACGGGATCACTCTGATGAGTGGTGA

Coding sequence (CDS)

ATGGGGGAGATCAGAACAATTATCCACCAAATTCTTCCCCAACAACTCCGCCATGTAACCTCCTCCAAATTCAACGCCATTTTTGACTTCGGGATTCTCTCTTCTCAAAAGTTGGTTTACATAATCGGCGAGAGCAACGGAGTCACCACCAACGAGCTCTACAGAGCCACCGAAACGTACCTCCACACAAAAATTCCGCTATCTGTGAAGCAGCTCGAAGCTTCCAAAGCCAAAGAGCAGAAGAATTTCTCCTTCAAAATCAGCAAAGGCGAGAGTTTAACCGACGAATTCCAAGGGATTCAAATCACCTGGGAATTGCATTCCATCCAAAAGGATCAGAAACCCAATTCTGAAAAACGGTACTACCAAATGAGCTTCCTCAAAAAACATAAAGATTTTGTCGACGATGTATACCTTCCGTACATCATGAACAGAGCAAACGCCATAGACGAAGGAAACAGAGTGGTGAAACTTTACTCTTTGGGGTATGATGAATCCTCCATTGTTTTGCGGAATACGTGCTCGTTTGAGACATTGGCTATGGATCCGGAGAAGAAGAAGGAGGTGATGGATGATCTGGACAGATTTGTGAGGAGGAAGGATTTTTACAGGAGGCTTGGGAGGGCCTGGAAAAGAGGGTATATTTTGTATGGCCCACCGGGAACTGGGAAATCCAGCTTGGTGCTGGCCATGGCTAATTATCTCAAGTTTAGTATCTACGATTTGGAGCTCACGAGCGTTGACAGTAACTCAGAGTTCAGGGAGATGATGCTGTGCACTGCTGACCGATCAATTATTGTCATTGAAGATATTGATTGCAGCACTGAGCTTGAGGATCGTGAATGTGACGATTATGGCGACCGCAAAGGAAAGCTGACTTTGTCTGGAGTTCTAAACGCCATTGATGGGCTATGGTCGAGCTGTGGGGATGCGAGGATTATAGTTTTCACGACGAATCATAAGGAGAAGTTGGACCCAGCACTGTTAAGGCCAGGGCGAATGGATATGCACATCCACATGACTTATTTGACCCCATCTGGATTTCAGATTCTGGCCTCAAACTATCTGCAGATTAAAAGCCATGATCGATTCAAAGAAATCGAAGAGCTGATAATGGAGGTGGAGGTCACGCCGGCGGAAATTGCAGAGGAGCTCATGAAAAGCGACGATGCTGACGTGGCCCTTGGAGCAGTGGTGGAATTCATCAATGGCAAGAAGAGGAAGAGGGTGGAAAAGGACGAACGGGACCCCCAAGAAGACATTGAGGAAGAAGACCCGAAGGAGTTCAAACAAAAAAAGGGAAACACAAGAAGAAGAAGGCACATGAGATGGAGACGGGATCACTCTGATGAGTGGTGA

Protein sequence

MGEIRTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETYLHTKIPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQKPNSEKRYYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYSLGYDESSIVLRNTCSFETLAMDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGKSSLVLAMANYLKFSIYDLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDRECDDYGDRKGKLTLSGVLNAIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMTYLTPSGFQILASNYLQIKSHDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGKKRKRVEKDERDPQEDIEEEDPKEFKQKKGNTRRRRHMRWRRDHSDEW
Homology
BLAST of MC09g_new0496 vs. ExPASy Swiss-Prot
Match: Q147F9 (AAA-ATPase At3g50940 OS=Arabidopsis thaliana OX=3702 GN=At3g50940 PE=2 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.5e-109
Identity = 200/419 (47.73%), Postives = 293/419 (69.93%), Query Frame = 0

Query: 5   RTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETYLHTK 64
           R+++   +P ++    S  F   F +    S ++  +I E  G   N+++ A E YL TK
Sbjct: 30  RSVVQDYMPNEVHEYISHGFRRFFSY---FSYQMTAVIEEFGGFEHNQVFEAAEAYLSTK 89

Query: 65  IPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQKP--------- 124
           I  S ++++ +K ++Q N+S  + + E + D F G++++W L     D+K          
Sbjct: 90  ISNSTRRIKVNKLEKQSNYSVTVERDEEVVDIFDGVKLSWILVCRHVDKKDFRNPRDLNS 149

Query: 125 --NSEKRYYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLY---SLGYDESSIVL 184
              SE R Y++SF KK K+ V + YLP+++ +A +I +  + +K++   S   + +S+ L
Sbjct: 150 TLKSEVRSYELSFRKKFKNMVLESYLPFVVEQAASIKQKFKTLKIFTVDSYSVEWTSVTL 209

Query: 185 RNTCSFETLAMDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGKSSLVLA 244
            +  +F TLA+DPE KK +++DLDRFV+RK FY R+G+AWKRGY+LYGPPGTGKSSL+ A
Sbjct: 210 DHPSTFRTLALDPEVKKNLVEDLDRFVQRKGFYGRVGKAWKRGYLLYGPPGTGKSSLIAA 269

Query: 245 MANYLKFSIYDLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDRECD-DYGDRK 304
           +AN+L F IYDL+LTS+++N+E R +++ TA+RSI+V+EDIDCS EL+DR  D +  D  
Sbjct: 270 IANHLNFDIYDLDLTSLNNNAELRRLLMSTANRSILVVEDIDCSIELKDRSTDQENNDPL 329

Query: 305 GK-LTLSGVLNAIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMTYLTPSGF 364
            K +TLSG+LN +DGLWSSCG+ RIIVFTTN++EKLDPALLRPGRMDMHIHM+Y TP+ F
Sbjct: 330 HKTVTLSGLLNFVDGLWSSCGNERIIVFTTNYREKLDPALLRPGRMDMHIHMSYCTPAAF 389

Query: 365 QILASNYLQIKSHDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGKKR 408
           ++LASNYL+I+ H  F++IEE I E+EVTPAE+AE+LM+SD  D  L  +VEF+  KK+
Sbjct: 390 KVLASNYLEIQDHILFEQIEEFIREIEVTPAEVAEQLMRSDSVDKVLQGLVEFLKAKKQ 445

BLAST of MC09g_new0496 vs. ExPASy Swiss-Prot
Match: Q8VZG2 (Protein HYPER-SENSITIVITY-RELATED 4 OS=Arabidopsis thaliana OX=3702 GN=HSR4 PE=2 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 2.2e-108
Identity = 215/458 (46.94%), Postives = 306/458 (66.81%), Query Frame = 0

Query: 5   RTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETYLHTK 64
           R+++   LP ++ H  S  F +I  FG  SSQ +  II E  G   NE++ A E YL TK
Sbjct: 76  RSLVQDYLPDEVHHYISYGFRSI--FGYFSSQ-MTIIIEEFEGFAHNEVFEAAEAYLATK 135

Query: 65  IPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQKP--------- 124
           I  S K+++ SK +++ N++  + + E + D + G++  W LH    + K          
Sbjct: 136 ISPSNKRIKVSKHEKENNYNVTVERDEEVVDTYNGVKFQWILHCRHVESKHFHNPRDLNS 195

Query: 125 --NSEKRYYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYSLGYDE-------- 184
              SE R ++++F KK KD   + YLP+++ RA  + +  + +K+++L  +         
Sbjct: 196 TLRSEVRSFELNFHKKFKDVALESYLPFMVKRATLMKQEKKTLKIFTLSPENMYGNYSDA 255

Query: 185 -SSIVLRNTCSFETLAMDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGK 244
            +S+ L +  +F+TLAMD + K  VM+DLD+FV+R+DFY+R+G+AWKRGY+LYGPPGTGK
Sbjct: 256 WTSVTLDHPSTFKTLAMDSDVKTSVMEDLDKFVKRRDFYKRVGKAWKRGYLLYGPPGTGK 315

Query: 245 SSLVLAMANYLKFSIYDLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELED----- 304
           SSL+ AMAN+L F IYDLELT+V++NSE R +++ TA+RSI+++EDIDCS EL+D     
Sbjct: 316 SSLIAAMANHLNFDIYDLELTAVNNNSELRRLLIATANRSILIVEDIDCSLELKDRTSDE 375

Query: 305 --RECDDYGD-RKGKLTLSGVLNAIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDM 364
             RE DD  D R  K+TLSG+LN IDGLWSSCGD RII+FTTN+KEKLD ALLRPGRMDM
Sbjct: 376 PPRESDDIEDPRYKKVTLSGLLNFIDGLWSSCGDERIIIFTTNYKEKLDAALLRPGRMDM 435

Query: 365 HIHMTYLTPSGFQILASNYLQIKSHDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALG 424
           HIHM+Y TPS F+ LA NYL+IK H  F +IEE I   EVTPAE+AE+LM++D  D  L 
Sbjct: 436 HIHMSYCTPSTFKALALNYLEIKEHRLFSKIEEGIEATEVTPAEVAEQLMRNDSVDKVLE 495

Query: 425 AVVEFINGKKRKRVEKDERDPQEDIEEEDPKEFKQKKG 435
            ++EF+   K K++E +E+D  +  ++E   + K K+G
Sbjct: 496 GLIEFL---KVKKIE-NEQDKAKTEKQELENKKKTKEG 526

BLAST of MC09g_new0496 vs. ExPASy Swiss-Prot
Match: Q8GW96 (AAA-ATPase At2g18193 OS=Arabidopsis thaliana OX=3702 GN=At2g18193 PE=2 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 1.9e-104
Identity = 210/458 (45.85%), Postives = 304/458 (66.38%), Query Frame = 0

Query: 5   RTIIHQILPQQLRHVTSSKFNAIFD-FGILSSQKLVYIIGESNGVTTNELYRATETYLHT 64
           R+++H  +P++LR    S F+++ D F    S+ L  II E+ G+  N+++ A E YL +
Sbjct: 29  RSMLHDFVPEKLR----SYFSSLLDRFFTPKSKYLTVIIDENFGLNRNQVFDAAEMYLRS 88

Query: 65  KIPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQKPNSEKRYYQ 124
           KI    ++L   K  +QK+F+  I +GE + D F+  ++ W  +   +++K +  KRYY+
Sbjct: 89  KIGPETERLRVGKIPKQKHFTISIERGEEILDTFEESEVKWS-YVQSENEKGDKVKRYYE 148

Query: 125 MSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYS------------LGYDESSIVLR 184
           ++F KK +D V + YL +++  +  I    RVVKLYS             G +   I L 
Sbjct: 149 LTFEKKLRDKVLNSYLTHVVAESEEIKRNLRVVKLYSRDVYASDDDDGMAGGNWGCINLE 208

Query: 185 NTCSFETLAMDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGKSSLVLAM 244
           +  +F+TLAMDP  KK+++DDL+RF++RK+FY+R+G+AWKRGY+LYGPPGTGKSSL+ AM
Sbjct: 209 HPSTFDTLAMDPNAKKKIIDDLERFLKRKEFYKRVGKAWKRGYLLYGPPGTGKSSLIAAM 268

Query: 245 ANYLKFSIYDLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDRECDDYGDR--K 304
           ANYLKF ++DLEL+S+  N E + ++L T +RSI+VIEDIDC+ E+ DRE ++  D   K
Sbjct: 269 ANYLKFDVFDLELSSIYDNGELKRVLLSTTNRSILVIEDIDCNAEVRDREAENQEDEQIK 328

Query: 305 GKLTLSGVLNAIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMTYLTPSGFQ 364
           GK+TLSG+LN IDGLWSS GD RIIVFTTNHKE+LDPALLRPGRMD+HI+M+Y T  GF+
Sbjct: 329 GKVTLSGILNFIDGLWSSFGDERIIVFTTNHKERLDPALLRPGRMDVHINMSYCTGLGFR 388

Query: 365 ILASNYLQIK--SHDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGKKR 424
            L SNYL +   +H   +EIE L+   EVTPAE+AEELM+ DD DV L  V+ F+  +K 
Sbjct: 389 TLVSNYLGLDGLNHPLCEEIEALVDSTEVTPAELAEELMQDDDTDVVLRGVISFVEKRKV 448

Query: 425 KRVEKDERDPQEDIEEEDPKE-------FKQKKGNTRR 439
           +R +  +        ++D K+        K+KKG  ++
Sbjct: 449 ERSKTKKEVSICKATDDDEKQNGSLGCVKKKKKGGKQK 481

BLAST of MC09g_new0496 vs. ExPASy Swiss-Prot
Match: F4IQG2 (AAA-ATPase At2g18190 OS=Arabidopsis thaliana OX=3702 GN=At2g18190 PE=2 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 1.2e-101
Identity = 198/421 (47.03%), Postives = 278/421 (66.03%), Query Frame = 0

Query: 5   RTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETYLHTK 64
           R++ +  +P++LR   +   N  F      S+ L  +I E  G   N+++ A E YL  K
Sbjct: 30  RSLFNDEVPERLRSYITDLLNRFF---TPKSKNLTMVIDEIIGFKRNQVFDAAEVYLRNK 89

Query: 65  IPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQKPNSEKRYYQM 124
           I     +L   K  +QK+F+  I KGE + D F+  ++ W  +   +++    EKRYY++
Sbjct: 90  IGPETARLRVGKLPKQKHFTIYIEKGEEILDTFENSELRW-TYVESENEASQKEKRYYEL 149

Query: 125 SFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYS------------LGYDESSIVLRN 184
           +F KK +D V + YL +++  +       R VKLYS             G     I L +
Sbjct: 150 TFEKKLRDKVMNSYLSHVVAESEETKRDLRAVKLYSRDVRASKDDDGMAGAGWGCINLEH 209

Query: 185 TCSFETLAMDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGKSSLVLAMA 244
             +FETLAMDP  KK+++DD++RF++R++FY+R+G+AWKRGY+LYGPPGTGKSSL+ AMA
Sbjct: 210 PSTFETLAMDPGAKKKIIDDMERFLKRREFYKRVGKAWKRGYLLYGPPGTGKSSLIAAMA 269

Query: 245 NYLKFSIYDLELTSVDSNSEFREMMLCTADRSIIVIEDIDCST-ELEDRECDDYGDRK-- 304
           NYLKF ++DLEL+S+  N++ + ++L T +RSI+VIEDIDCS+ E+ DRE D+Y + +  
Sbjct: 270 NYLKFDVFDLELSSIYENAQLKSILLSTTNRSILVIEDIDCSSAEVVDREADEYQEYEEG 329

Query: 305 --GKLTLSGVLNAIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMTYLTPSG 364
             G++TLSG+LN +DGLWSS GD RIIVFTTNHKE+LDPALLRPGRMDMHI+M+Y T  G
Sbjct: 330 YYGRVTLSGLLNFVDGLWSSFGDERIIVFTTNHKERLDPALLRPGRMDMHINMSYCTGLG 389

Query: 365 FQILASNYLQI--KSHDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGK 407
           F+ L SNYL +   +H   +EIE LI   EVTPAE+AEELM+ DD DV L  VV F+  +
Sbjct: 390 FRTLVSNYLGLGGLNHPLCEEIEALIDSTEVTPAELAEELMQEDDTDVVLRGVVSFVENR 446

BLAST of MC09g_new0496 vs. ExPASy Swiss-Prot
Match: Q9FN75 (AAA-ATPase At5g17760 OS=Arabidopsis thaliana OX=3702 GN=At5g17760 PE=3 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 1.0e-100
Identity = 205/468 (43.80%), Postives = 298/468 (63.68%), Query Frame = 0

Query: 4   IRTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETYLHT 63
           IR++ H+++P  L+        ++F     SS  L   I + N    NE+YRA +TYL T
Sbjct: 27  IRSMAHELIPAPLQDFIYRTLRSLFFRS--SSSTLTLTIDDDNMGMNNEIYRAAQTYLST 86

Query: 64  KIPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQK--------- 123
           KI     +L  SK  + K+ +  +S GE + D ++ +Q+ W   +   D+K         
Sbjct: 87  KISPDAVRLRISKGHKDKHVNLYLSDGEIVNDVYEDVQLVWRFVTDGGDKKGGGGGVGGR 146

Query: 124 -----------PNSEKRYYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYSL-G 183
                       + +  Y+++SF KKHKD + + Y+PYI ++A  I +  R++ L+SL  
Sbjct: 147 GGGGGRRGGMDDDGKSEYFELSFDKKHKDLILNSYVPYIESKAKEIRDERRILMLHSLNS 206

Query: 184 YDESSIVLRNTCSFETLAMDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGT 243
               S++L +  +FET+AM+ + K++V++DLDRF+RRK+FY+R+G+AWKRGY+LYGPPGT
Sbjct: 207 LRWESVILEHPSTFETMAMEDDLKRDVIEDLDRFIRRKEFYKRVGKAWKRGYLLYGPPGT 266

Query: 244 GKSSLVLAMANYLKFSIYDLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDR-- 303
           GKSSLV AMANYLKF +YDL+L SV  +S+ R ++L T +RSI+VIEDIDC+ +L +R  
Sbjct: 267 GKSSLVAAMANYLKFDVYDLQLASVMRDSDLRRLLLATRNRSILVIEDIDCAVDLPNRIE 326

Query: 304 ---ECDDYGDRKGKLTLSGVLNAIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMH 363
              E  + G+ +G LTLSG+LN IDGLWSSCGD RII+FTTNHK++LDPALLRPGRMDMH
Sbjct: 327 QPVEGKNRGESQGPLTLSGLLNFIDGLWSSCGDERIIIFTTNHKDRLDPALLRPGRMDMH 386

Query: 364 IHMTYLTPSGFQILASNYLQIKS----HDRFKEIEELIMEVEVTPAEIAEELMKSDDADV 423
           I+M + +  GF+ LASNYL +      H  F EIE LI    +TPA++AEELMKS+DADV
Sbjct: 387 IYMGHCSFQGFKTLASNYLGLSDAAMPHRLFPEIERLIDGEVMTPAQVAEELMKSEDADV 446

Query: 424 ALGAVVEFINGKKRKRVEKDE---RDPQEDIEEEDPKEFKQKKGNTRR 439
           AL  +V  +   + K  E +    +  +  +E E+ +     +G+ R+
Sbjct: 447 ALEGLVNVLEKMRLKSKESNPVMMKQKESRLEMEEMRLKSDTEGSPRK 492

BLAST of MC09g_new0496 vs. NCBI nr
Match: XP_022151910.1 (AAA-ATPase At3g50940-like [Momordica charantia])

HSP 1 Score: 888 bits (2295), Expect = 0.0
Identity = 452/452 (100.00%), Postives = 452/452 (100.00%), Query Frame = 0

Query: 1   MGEIRTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETY 60
           MGEIRTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETY
Sbjct: 31  MGEIRTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETY 90

Query: 61  LHTKIPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQKPNSEKR 120
           LHTKIPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQKPNSEKR
Sbjct: 91  LHTKIPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQKPNSEKR 150

Query: 121 YYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYSLGYDESSIVLRNTCSFETLA 180
           YYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYSLGYDESSIVLRNTCSFETLA
Sbjct: 151 YYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYSLGYDESSIVLRNTCSFETLA 210

Query: 181 MDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGKSSLVLAMANYLKFSIY 240
           MDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGKSSLVLAMANYLKFSIY
Sbjct: 211 MDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGKSSLVLAMANYLKFSIY 270

Query: 241 DLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDRECDDYGDRKGKLTLSGVLNA 300
           DLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDRECDDYGDRKGKLTLSGVLNA
Sbjct: 271 DLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDRECDDYGDRKGKLTLSGVLNA 330

Query: 301 IDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMTYLTPSGFQILASNYLQIKS 360
           IDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMTYLTPSGFQILASNYLQIKS
Sbjct: 331 IDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMTYLTPSGFQILASNYLQIKS 390

Query: 361 HDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGKKRKRVEKDERDPQED 420
           HDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGKKRKRVEKDERDPQED
Sbjct: 391 HDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGKKRKRVEKDERDPQED 450

Query: 421 IEEEDPKEFKQKKGNTRRRRHMRWRRDHSDEW 452
           IEEEDPKEFKQKKGNTRRRRHMRWRRDHSDEW
Sbjct: 451 IEEEDPKEFKQKKGNTRRRRHMRWRRDHSDEW 482

BLAST of MC09g_new0496 vs. NCBI nr
Match: XP_022151927.1 (AAA-ATPase At3g50940-like [Momordica charantia])

HSP 1 Score: 573 bits (1478), Expect = 2.53e-200
Identity = 320/468 (68.38%), Postives = 369/468 (78.85%), Query Frame = 0

Query: 1   MGEIRTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETY 60
           + E  TI+ QI+PQ+LR   S KFNA+F  G LSS  +  II E+NGV  NELYRA+ETY
Sbjct: 25  ISETNTILCQIIPQKLRQEISFKFNAMF--GPLSSH-MTLIIDENNGVAINELYRASETY 84

Query: 61  LHTKIPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQ------- 120
           L TKIP S+K L+ASK+  + N SFKI+KG++LTDEF+GI++TWEL S +K         
Sbjct: 85  LTTKIPTSLKHLKASKSPGENNLSFKINKGDNLTDEFEGIEVTWELISTEKQSTYFDFDS 144

Query: 121 ----KPNSEKRYYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYSL--GYDES- 180
                   EKR+Y+MSF KKH+D V ++YLPYI+ RA AI+E NR VK+++L  GY    
Sbjct: 145 YSQTSETIEKRHYRMSFHKKHRDLVMNIYLPYILKRAKAIEEENRAVKIHALMGGYGNGD 204

Query: 181 SIVLRNTCSFETLAMDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGKSS 240
           SIVL++TC FE LAMDP+KKK+VMDDL+RFVRR+DFYRR+G+AWKRGY+LYGPPGTGKSS
Sbjct: 205 SIVLQSTCCFEKLAMDPKKKKDVMDDLERFVRRRDFYRRIGKAWKRGYLLYGPPGTGKSS 264

Query: 241 LVLAMANYLKFSIYDLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDRECDDYG 300
           LV AMA+YLKF+IYDLELTSV SNS  R M+L T DRSIIVIEDIDCS ELEDR    YG
Sbjct: 265 LVAAMADYLKFNIYDLELTSVQSNSALRTMLLSTTDRSIIVIEDIDCSAELEDRNNGGYG 324

Query: 301 DRKG-KLTLSGVLNAIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMTYLTP 360
                KLTLSGVLNAIDGLWSSCGDARIIVFTTNH+EKLDPALLRPGRMDMHIHMTYLTP
Sbjct: 325 GAGDTKLTLSGVLNAIDGLWSSCGDARIIVFTTNHREKLDPALLRPGRMDMHIHMTYLTP 384

Query: 361 SGFQILASNYLQIKSHDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGK 420
           SGFQILASNYLQIK+HDRF+EIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGK
Sbjct: 385 SGFQILASNYLQIKNHDRFREIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGK 444

Query: 421 KRKRVEKD-------ERDPQE--DIEEEDPKEFKQKKGNTRRRRHMRW 444
           KRK++EK+       + D Q   +IEEED      KK N RRR   RW
Sbjct: 445 KRKKMEKELCNSELIKNDEQSLRNIEEEDQNV---KKRNKRRR--TRW 484

BLAST of MC09g_new0496 vs. NCBI nr
Match: XP_038892960.1 (AAA-ATPase At3g50940-like [Benincasa hispida])

HSP 1 Score: 538 bits (1386), Expect = 2.69e-186
Identity = 296/459 (64.49%), Postives = 356/459 (77.56%), Query Frame = 0

Query: 1   MGEIRTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETY 60
           + E  +II Q +PQ+LR   SSKF+AIF  G +SSQ +V I+ E+NG+  NEL+RA+ETY
Sbjct: 31  ISETHSIISQFIPQKLRDQISSKFSAIF--GSISSQ-IVLIVEENNGIAINELFRASETY 90

Query: 61  LHTKIPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQ------- 120
           L TKI  S+K L+ASKA  + N +FK++KG+ L D F+ I+I WEL S +K         
Sbjct: 91  LSTKISQSLKHLKASKAPGETNLTFKMNKGDVLIDVFETIEIAWELISTEKQSTYFDFDI 150

Query: 121 ----KPNSEKRYYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYSL--GYD-ES 180
                   EKR+YQ+SF KKHKD V  +YL YI++RA AI+E NRVVKLY+L  GY    
Sbjct: 151 ATQTSETIEKRHYQISFDKKHKDLVMKIYLEYILDRAKAIEEENRVVKLYALMGGYAIGD 210

Query: 181 SIVLRNTCSFETLAMDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGKSS 240
           SIVL+N+CSFE LAMD +KKKE+MDDLDRF+RR+DFY+R+G+AWKRGY+LYGPPGTGKSS
Sbjct: 211 SIVLQNSCSFENLAMDLKKKKELMDDLDRFMRRRDFYKRIGKAWKRGYLLYGPPGTGKSS 270

Query: 241 LVLAMANYLKFSIYDLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDRECDDYG 300
           LV AMA+YLKF+IYDLELTSV +NS  R M+L TADRSI VIEDIDCS EL DR      
Sbjct: 271 LVAAMADYLKFNIYDLELTSVQTNSALRTMLLSTADRSIFVIEDIDCSAELHDRTNGGRD 330

Query: 301 DRKGKLTLSGVLNAIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMTYLTPS 360
               +LTLSGVLN IDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMH+HMTYLTPS
Sbjct: 331 GGDSQLTLSGVLNVIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHVHMTYLTPS 390

Query: 361 GFQILASNYLQIKSHDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGKK 420
           GF+ILASNYLQI  H RFKEI++LIMEVEVTPAEIAEELMKSDDADVAL +VVEF+NGKK
Sbjct: 391 GFEILASNYLQINHHQRFKEIQDLIMEVEVTPAEIAEELMKSDDADVALESVVEFVNGKK 450

Query: 421 RKRVEKD-ERDPQEDIEEEDPKEF-----KQKKGNTRRR 439
           +K++EK+   DP E+++ +  ++      K KK N RRR
Sbjct: 451 KKKMEKECNSDPIENVDGQISRDIEKEGLKLKKRNKRRR 486

BLAST of MC09g_new0496 vs. NCBI nr
Match: KAB1213001.1 (putative mitochondrial chaperone bcs1 [Morella rubra])

HSP 1 Score: 461 bits (1186), Expect = 5.72e-156
Identity = 249/459 (54.25%), Postives = 331/459 (72.11%), Query Frame = 0

Query: 1   MGEIRTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETY 60
           + E++T+  Q++PQ LR    SK   +F  G  SSQ L  II E NG++ NE+Y+A+E Y
Sbjct: 25  INEVQTMTSQLIPQHLREKLFSKHGGLF--GNFSSQ-LTLIIDEYNGLSMNEIYQASEVY 84

Query: 61  LHTKIPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQK------ 120
           L T+   S+++L+ SKA  +KN S  I+KGE + D ++GI +TWE    +K +       
Sbjct: 85  LSTRDTPSIERLKVSKAPREKNLSITINKGEKINDVYEGIHLTWEFVCTEKQKSYYDSES 144

Query: 121 -----PNSEKRYYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYSLG--YDE-- 180
                 NSE R +Q++F KK+K+ V   YLPY+++RA AI E ++VVKLYSLG  Y E  
Sbjct: 145 YSCTTENSEHRSFQLTFTKKYKEKVVSSYLPYVLDRAKAIKEESKVVKLYSLGSFYGEYN 204

Query: 181 ----SSIVLRNTCSFETLAMDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPG 240
                S+ L +  +F+TLAMDPE K+E+MDDL+RFV+R++FYRR+G+AWKRGY+LYGPPG
Sbjct: 205 GGPWGSVHLDHPSTFDTLAMDPELKQELMDDLERFVKRREFYRRVGKAWKRGYLLYGPPG 264

Query: 241 TGKSSLVLAMANYLKFSIYDLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDRE 300
           TGKSSL+ AMANYLKF IYD+EL S+ SNSE R +++ TA+RSI+VIEDIDCS EL+DR 
Sbjct: 265 TGKSSLIAAMANYLKFDIYDMELASLHSNSELRRLLVSTANRSILVIEDIDCSIELQDRR 324

Query: 301 CDDYGDRKGKLTLSGVLNAIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMT 360
              +   + +LTLSG+LN IDGLWSSCGD RIIVFTTNHK+KLDPALLRPGRMDMHIH++
Sbjct: 325 HAGFDHTESQLTLSGLLNFIDGLWSSCGDERIIVFTTNHKDKLDPALLRPGRMDMHIHLS 384

Query: 361 YLTPSGFQILASNYLQIKSHDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEF 420
           Y TPSGF+ILASNYL I+SH  F EIE L+ EVEVTPAE+AEELMK++D DV L  +V F
Sbjct: 385 YCTPSGFKILASNYLSIQSHCLFTEIEGLVEEVEVTPAEVAEELMKNEDPDVLLPRLVAF 444

Query: 421 INGKKRKRVEKDERDPQEDIEEEDPKEFKQKKGNTRRRR 440
           +  KK+ +  ++    +E ++EE  K  K K+   ++ R
Sbjct: 445 LEQKKQVKNTEESNIEEEKVKEEVEKSPKMKETGKKKLR 480

BLAST of MC09g_new0496 vs. NCBI nr
Match: XP_028113418.1 (AAA-ATPase At3g50940-like [Camellia sinensis])

HSP 1 Score: 456 bits (1173), Expect = 4.34e-154
Identity = 243/449 (54.12%), Postives = 324/449 (72.16%), Query Frame = 0

Query: 1   MGEIRTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETY 60
           + E+++I +Q++P+QL+    SK   +   G  SSQ  +YI   +NG   N+++ A+E Y
Sbjct: 32  LNEVQSITYQLIPKQLQEKIISKLGGLI--GCSSSQMTLYIDESNNGQPINQVFEASEIY 91

Query: 61  LHTKIPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQKP----- 120
           L  KI  SV++L  SKA  QK+    I+ GE + D FQ IQ+TW+L    K QK      
Sbjct: 92  LRAKISPSVERLRVSKAPGQKHICVTINPGEQIVDTFQDIQLTWQLKCT-KSQKTGPDYD 151

Query: 121 -------NSEKRYYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYSLGYDESSI 180
                  +SE+R +++SF KK+++ V   YLPY++ R+ A+ E N+V+KL+S G   SS+
Sbjct: 152 SGNNDFFHSERRSFELSFHKKYREIVLSSYLPYVLERSKAMKEENKVMKLHSYGGSCSSV 211

Query: 181 VLRNTCSFETLAMDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGKSSLV 240
            L +  +F+TLAMDP  KKEVMDDLDRFV+RKDFYRR+G+AWKRGY+LYGPPGTGKSSL+
Sbjct: 212 NLDHPSTFDTLAMDPTLKKEVMDDLDRFVKRKDFYRRVGKAWKRGYLLYGPPGTGKSSLI 271

Query: 241 LAMANYLKFSIYDLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDRECDDYGDR 300
            AMANYLKF IYD+ELT + SNS+ R +++ TA+RSI+VIEDIDCST  ++R+     D 
Sbjct: 272 AAMANYLKFHIYDVELTRLRSNSDLRNLLVSTANRSILVIEDIDCSTNFQNRQAGGNNDG 331

Query: 301 KGKLTLSGVLNAIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMTYLTPSGF 360
             +LTLSG+LN IDGLWSSCGD +II+FTTNHKE+LDPALLRPGRMDMHIHM+Y TP GF
Sbjct: 332 NKRLTLSGLLNFIDGLWSSCGDEKIIIFTTNHKEELDPALLRPGRMDMHIHMSYCTPCGF 391

Query: 361 QILASNYLQIKSHDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGKKRK 420
           +ILASNYL IK+H  F EIE+L+ EVEVTPAEIAEELM+S++ D ALG +V+F+N KK +
Sbjct: 392 KILASNYLGIKNHPMFTEIEKLVTEVEVTPAEIAEELMRSEETDKALGGLVKFLNVKKTE 451

Query: 421 RVEKDERDPQE--DIEEEDPKEFKQKKGN 435
             + +    QE  +IE+E  ++ K   GN
Sbjct: 452 HDKANAEGEQEVGEIEKEVSEQAKDSPGN 477

BLAST of MC09g_new0496 vs. ExPASy TrEMBL
Match: A0A6J1DCI1 (AAA-ATPase At3g50940-like OS=Momordica charantia OX=3673 GN=LOC111019763 PE=3 SV=1)

HSP 1 Score: 888 bits (2295), Expect = 0.0
Identity = 452/452 (100.00%), Postives = 452/452 (100.00%), Query Frame = 0

Query: 1   MGEIRTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETY 60
           MGEIRTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETY
Sbjct: 31  MGEIRTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETY 90

Query: 61  LHTKIPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQKPNSEKR 120
           LHTKIPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQKPNSEKR
Sbjct: 91  LHTKIPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQKPNSEKR 150

Query: 121 YYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYSLGYDESSIVLRNTCSFETLA 180
           YYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYSLGYDESSIVLRNTCSFETLA
Sbjct: 151 YYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYSLGYDESSIVLRNTCSFETLA 210

Query: 181 MDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGKSSLVLAMANYLKFSIY 240
           MDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGKSSLVLAMANYLKFSIY
Sbjct: 211 MDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGKSSLVLAMANYLKFSIY 270

Query: 241 DLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDRECDDYGDRKGKLTLSGVLNA 300
           DLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDRECDDYGDRKGKLTLSGVLNA
Sbjct: 271 DLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDRECDDYGDRKGKLTLSGVLNA 330

Query: 301 IDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMTYLTPSGFQILASNYLQIKS 360
           IDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMTYLTPSGFQILASNYLQIKS
Sbjct: 331 IDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMTYLTPSGFQILASNYLQIKS 390

Query: 361 HDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGKKRKRVEKDERDPQED 420
           HDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGKKRKRVEKDERDPQED
Sbjct: 391 HDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGKKRKRVEKDERDPQED 450

Query: 421 IEEEDPKEFKQKKGNTRRRRHMRWRRDHSDEW 452
           IEEEDPKEFKQKKGNTRRRRHMRWRRDHSDEW
Sbjct: 451 IEEEDPKEFKQKKGNTRRRRHMRWRRDHSDEW 482

BLAST of MC09g_new0496 vs. ExPASy TrEMBL
Match: A0A6J1DET2 (AAA-ATPase At3g50940-like OS=Momordica charantia OX=3673 GN=LOC111019781 PE=3 SV=1)

HSP 1 Score: 573 bits (1478), Expect = 1.23e-200
Identity = 320/468 (68.38%), Postives = 369/468 (78.85%), Query Frame = 0

Query: 1   MGEIRTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETY 60
           + E  TI+ QI+PQ+LR   S KFNA+F  G LSS  +  II E+NGV  NELYRA+ETY
Sbjct: 25  ISETNTILCQIIPQKLRQEISFKFNAMF--GPLSSH-MTLIIDENNGVAINELYRASETY 84

Query: 61  LHTKIPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQ------- 120
           L TKIP S+K L+ASK+  + N SFKI+KG++LTDEF+GI++TWEL S +K         
Sbjct: 85  LTTKIPTSLKHLKASKSPGENNLSFKINKGDNLTDEFEGIEVTWELISTEKQSTYFDFDS 144

Query: 121 ----KPNSEKRYYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYSL--GYDES- 180
                   EKR+Y+MSF KKH+D V ++YLPYI+ RA AI+E NR VK+++L  GY    
Sbjct: 145 YSQTSETIEKRHYRMSFHKKHRDLVMNIYLPYILKRAKAIEEENRAVKIHALMGGYGNGD 204

Query: 181 SIVLRNTCSFETLAMDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGKSS 240
           SIVL++TC FE LAMDP+KKK+VMDDL+RFVRR+DFYRR+G+AWKRGY+LYGPPGTGKSS
Sbjct: 205 SIVLQSTCCFEKLAMDPKKKKDVMDDLERFVRRRDFYRRIGKAWKRGYLLYGPPGTGKSS 264

Query: 241 LVLAMANYLKFSIYDLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDRECDDYG 300
           LV AMA+YLKF+IYDLELTSV SNS  R M+L T DRSIIVIEDIDCS ELEDR    YG
Sbjct: 265 LVAAMADYLKFNIYDLELTSVQSNSALRTMLLSTTDRSIIVIEDIDCSAELEDRNNGGYG 324

Query: 301 DRKG-KLTLSGVLNAIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMTYLTP 360
                KLTLSGVLNAIDGLWSSCGDARIIVFTTNH+EKLDPALLRPGRMDMHIHMTYLTP
Sbjct: 325 GAGDTKLTLSGVLNAIDGLWSSCGDARIIVFTTNHREKLDPALLRPGRMDMHIHMTYLTP 384

Query: 361 SGFQILASNYLQIKSHDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGK 420
           SGFQILASNYLQIK+HDRF+EIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGK
Sbjct: 385 SGFQILASNYLQIKNHDRFREIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGK 444

Query: 421 KRKRVEKD-------ERDPQE--DIEEEDPKEFKQKKGNTRRRRHMRW 444
           KRK++EK+       + D Q   +IEEED      KK N RRR   RW
Sbjct: 445 KRKKMEKELCNSELIKNDEQSLRNIEEEDQNV---KKRNKRRR--TRW 484

BLAST of MC09g_new0496 vs. ExPASy TrEMBL
Match: A0A7N2L9F5 (AAA domain-containing protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 467 bits (1201), Expect = 1.30e-158
Identity = 241/419 (57.52%), Postives = 314/419 (74.94%), Query Frame = 0

Query: 1   MGEIRTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETY 60
           + E++T+  Q++PQQL+   SSK   +F  G   S ++  II E NG++ NE+Y+A++ Y
Sbjct: 31  ISEVQTMTTQLIPQQLQEKLSSKLGGLF--GNNFSSQITLIIDEHNGLSINEIYQASDIY 90

Query: 61  LHTKIPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQKPN---- 120
           L T I  SVK L+ SKA  +KN S  I KGE + D F+GI++ WE  S +K QK N    
Sbjct: 91  LSTIITPSVKHLKVSKAPREKNLSITIDKGEKIIDVFEGIRLIWEFISTEK-QKSNFDYE 150

Query: 121 --------SEKRYYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYSLGYDESSI 180
                   SE+R   +S  KK+K+ V   YLPY+++R+ AI E N+VVKLYSLG     +
Sbjct: 151 TSLHSTETSEQRSIHLSLNKKYKEIVLSTYLPYVVDRSKAIKEENKVVKLYSLGNSYQDV 210

Query: 181 VLRNTCSFETLAMDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGKSSLV 240
            L +  +F+TLAMDP+ KKE+MDDLDRF++R++FYRR+G+AWKRGY+LYGPPGTGKSSL+
Sbjct: 211 NLDHPSTFDTLAMDPKLKKELMDDLDRFLKRREFYRRVGKAWKRGYLLYGPPGTGKSSLI 270

Query: 241 LAMANYLKFSIYDLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDRECDDYGDR 300
            AMANYLKF IYDLEL+++ SN++ R ++  TA+RSI+VIEDIDC+ EL+DR+   Y  R
Sbjct: 271 AAMANYLKFDIYDLELSNLHSNADLRRLIASTANRSILVIEDIDCTIELQDRQYGGYNQR 330

Query: 301 KGKLTLSGVLNAIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMTYLTPSGF 360
             +LTLSG+LN IDGLW+SCGD RIIVFTTNHK++LDPALLRPGRMDMHIHM+Y TPSGF
Sbjct: 331 DNQLTLSGLLNFIDGLWTSCGDERIIVFTTNHKDRLDPALLRPGRMDMHIHMSYSTPSGF 390

Query: 361 QILASNYLQIKSHDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGKKR 407
           +ILASNYL+I+SH  F EIE LI EVEVTPAE+AEELMKS+D D+AL  +V F+  KK+
Sbjct: 391 KILASNYLRIESHCLFTEIEGLIKEVEVTPAEVAEELMKSEDPDIALAGLVAFLQQKKK 446

BLAST of MC09g_new0496 vs. ExPASy TrEMBL
Match: A0A6A1VJH3 (Putative mitochondrial chaperone bcs1 OS=Morella rubra OX=262757 GN=CJ030_MR5G025812 PE=3 SV=1)

HSP 1 Score: 461 bits (1186), Expect = 2.77e-156
Identity = 249/459 (54.25%), Postives = 331/459 (72.11%), Query Frame = 0

Query: 1   MGEIRTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETY 60
           + E++T+  Q++PQ LR    SK   +F  G  SSQ L  II E NG++ NE+Y+A+E Y
Sbjct: 25  INEVQTMTSQLIPQHLREKLFSKHGGLF--GNFSSQ-LTLIIDEYNGLSMNEIYQASEVY 84

Query: 61  LHTKIPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQK------ 120
           L T+   S+++L+ SKA  +KN S  I+KGE + D ++GI +TWE    +K +       
Sbjct: 85  LSTRDTPSIERLKVSKAPREKNLSITINKGEKINDVYEGIHLTWEFVCTEKQKSYYDSES 144

Query: 121 -----PNSEKRYYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYSLG--YDE-- 180
                 NSE R +Q++F KK+K+ V   YLPY+++RA AI E ++VVKLYSLG  Y E  
Sbjct: 145 YSCTTENSEHRSFQLTFTKKYKEKVVSSYLPYVLDRAKAIKEESKVVKLYSLGSFYGEYN 204

Query: 181 ----SSIVLRNTCSFETLAMDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPG 240
                S+ L +  +F+TLAMDPE K+E+MDDL+RFV+R++FYRR+G+AWKRGY+LYGPPG
Sbjct: 205 GGPWGSVHLDHPSTFDTLAMDPELKQELMDDLERFVKRREFYRRVGKAWKRGYLLYGPPG 264

Query: 241 TGKSSLVLAMANYLKFSIYDLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDRE 300
           TGKSSL+ AMANYLKF IYD+EL S+ SNSE R +++ TA+RSI+VIEDIDCS EL+DR 
Sbjct: 265 TGKSSLIAAMANYLKFDIYDMELASLHSNSELRRLLVSTANRSILVIEDIDCSIELQDRR 324

Query: 301 CDDYGDRKGKLTLSGVLNAIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMT 360
              +   + +LTLSG+LN IDGLWSSCGD RIIVFTTNHK+KLDPALLRPGRMDMHIH++
Sbjct: 325 HAGFDHTESQLTLSGLLNFIDGLWSSCGDERIIVFTTNHKDKLDPALLRPGRMDMHIHLS 384

Query: 361 YLTPSGFQILASNYLQIKSHDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEF 420
           Y TPSGF+ILASNYL I+SH  F EIE L+ EVEVTPAE+AEELMK++D DV L  +V F
Sbjct: 385 YCTPSGFKILASNYLSIQSHCLFTEIEGLVEEVEVTPAEVAEELMKNEDPDVLLPRLVAF 444

Query: 421 INGKKRKRVEKDERDPQEDIEEEDPKEFKQKKGNTRRRR 440
           +  KK+ +  ++    +E ++EE  K  K K+   ++ R
Sbjct: 445 LEQKKQVKNTEESNIEEEKVKEEVEKSPKMKETGKKKLR 480

BLAST of MC09g_new0496 vs. ExPASy TrEMBL
Match: B9RMH6 (ATP binding protein, putative OS=Ricinus communis OX=3988 GN=RCOM_1080880 PE=3 SV=1)

HSP 1 Score: 455 bits (1171), Expect = 7.20e-154
Identity = 240/427 (56.21%), Postives = 312/427 (73.07%), Query Frame = 0

Query: 1   MGEIRTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETY 60
           + E++T+  Q++PQ+L+    +   ++F    L+S KL  II E NG T NE+Y+A++ Y
Sbjct: 34  LNEVQTMTAQLIPQKLQDKIMASLGSLFR---LNSCKLTLIIDEYNGFTINEIYQASQAY 93

Query: 61  LHTKIPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQ--------KD 120
           L T+I  SV QL+ SKA  +KNF+  I+KG+ +TDEF+GIQ+ WE  S +         D
Sbjct: 94  LSTRITPSVDQLKVSKAPREKNFTVTINKGQRITDEFEGIQVAWEFSSTETQTAASDYSD 153

Query: 121 QKPNSEKRYYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYSLGYDE------S 180
               SE++ + + F K+HKD V +VYLPY++ R+ A+ E N+ +KLYSL   E       
Sbjct: 154 STEKSERKLFLLCFNKEHKDAVLNVYLPYVLERSKALKEENKAIKLYSLFGGEYYEGPWG 213

Query: 181 SIVLRNTCSFETLAMDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGKSS 240
           SI L +  +F+T+AMDP  K+EVMDDLDRFV R++FYRR+GR WKRGY+LYGPPGTGKSS
Sbjct: 214 SINLDHPSTFDTIAMDPRLKQEVMDDLDRFVIRREFYRRVGRPWKRGYLLYGPPGTGKSS 273

Query: 241 LVLAMANYLKFSIYDLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDRECDDYG 300
           L+ AMANYLKF+IYDLELTS+ SNSE R ++  T +RSI+VIEDIDCS +L+DR+  +  
Sbjct: 274 LIAAMANYLKFNIYDLELTSISSNSELRRLLTSTGNRSILVIEDIDCSIKLQDRQNGENN 333

Query: 301 DRKGKLTLSGVLNAIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMTYLTPS 360
               +LTLSG+LN IDGLWSSCGD +IIVFTTN+K+KLDPALLRPGRMDMHIHM+Y T S
Sbjct: 334 PGDSQLTLSGLLNFIDGLWSSCGDEKIIVFTTNYKDKLDPALLRPGRMDMHIHMSYCTTS 393

Query: 361 GFQILASNYLQIKSHDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGKK 412
           GF+ILA NYL+IK+H  F EIE+LI EVEVTPAE+AEELMK  D D+ L  +  F+ GKK
Sbjct: 394 GFKILAFNYLKIKTHCLFTEIEKLIEEVEVTPAEVAEELMKGGDVDLVLKGLQGFLQGKK 453

BLAST of MC09g_new0496 vs. TAIR 10
Match: AT3G50940.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 397.9 bits (1021), Expect = 1.1e-110
Identity = 200/419 (47.73%), Postives = 293/419 (69.93%), Query Frame = 0

Query: 5   RTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETYLHTK 64
           R+++   +P ++    S  F   F +    S ++  +I E  G   N+++ A E YL TK
Sbjct: 30  RSVVQDYMPNEVHEYISHGFRRFFSY---FSYQMTAVIEEFGGFEHNQVFEAAEAYLSTK 89

Query: 65  IPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQKP--------- 124
           I  S ++++ +K ++Q N+S  + + E + D F G++++W L     D+K          
Sbjct: 90  ISNSTRRIKVNKLEKQSNYSVTVERDEEVVDIFDGVKLSWILVCRHVDKKDFRNPRDLNS 149

Query: 125 --NSEKRYYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLY---SLGYDESSIVL 184
              SE R Y++SF KK K+ V + YLP+++ +A +I +  + +K++   S   + +S+ L
Sbjct: 150 TLKSEVRSYELSFRKKFKNMVLESYLPFVVEQAASIKQKFKTLKIFTVDSYSVEWTSVTL 209

Query: 185 RNTCSFETLAMDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGKSSLVLA 244
            +  +F TLA+DPE KK +++DLDRFV+RK FY R+G+AWKRGY+LYGPPGTGKSSL+ A
Sbjct: 210 DHPSTFRTLALDPEVKKNLVEDLDRFVQRKGFYGRVGKAWKRGYLLYGPPGTGKSSLIAA 269

Query: 245 MANYLKFSIYDLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDRECD-DYGDRK 304
           +AN+L F IYDL+LTS+++N+E R +++ TA+RSI+V+EDIDCS EL+DR  D +  D  
Sbjct: 270 IANHLNFDIYDLDLTSLNNNAELRRLLMSTANRSILVVEDIDCSIELKDRSTDQENNDPL 329

Query: 305 GK-LTLSGVLNAIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMTYLTPSGF 364
            K +TLSG+LN +DGLWSSCG+ RIIVFTTN++EKLDPALLRPGRMDMHIHM+Y TP+ F
Sbjct: 330 HKTVTLSGLLNFVDGLWSSCGNERIIVFTTNYREKLDPALLRPGRMDMHIHMSYCTPAAF 389

Query: 365 QILASNYLQIKSHDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGKKR 408
           ++LASNYL+I+ H  F++IEE I E+EVTPAE+AE+LM+SD  D  L  +VEF+  KK+
Sbjct: 390 KVLASNYLEIQDHILFEQIEEFIREIEVTPAEVAEQLMRSDSVDKVLQGLVEFLKAKKQ 445

BLAST of MC09g_new0496 vs. TAIR 10
Match: AT3G50930.1 (cytochrome BC1 synthesis )

HSP 1 Score: 394.0 bits (1011), Expect = 1.6e-109
Identity = 215/458 (46.94%), Postives = 306/458 (66.81%), Query Frame = 0

Query: 5   RTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETYLHTK 64
           R+++   LP ++ H  S  F +I  FG  SSQ +  II E  G   NE++ A E YL TK
Sbjct: 76  RSLVQDYLPDEVHHYISYGFRSI--FGYFSSQ-MTIIIEEFEGFAHNEVFEAAEAYLATK 135

Query: 65  IPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQKP--------- 124
           I  S K+++ SK +++ N++  + + E + D + G++  W LH    + K          
Sbjct: 136 ISPSNKRIKVSKHEKENNYNVTVERDEEVVDTYNGVKFQWILHCRHVESKHFHNPRDLNS 195

Query: 125 --NSEKRYYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYSLGYDE-------- 184
              SE R ++++F KK KD   + YLP+++ RA  + +  + +K+++L  +         
Sbjct: 196 TLRSEVRSFELNFHKKFKDVALESYLPFMVKRATLMKQEKKTLKIFTLSPENMYGNYSDA 255

Query: 185 -SSIVLRNTCSFETLAMDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGK 244
            +S+ L +  +F+TLAMD + K  VM+DLD+FV+R+DFY+R+G+AWKRGY+LYGPPGTGK
Sbjct: 256 WTSVTLDHPSTFKTLAMDSDVKTSVMEDLDKFVKRRDFYKRVGKAWKRGYLLYGPPGTGK 315

Query: 245 SSLVLAMANYLKFSIYDLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELED----- 304
           SSL+ AMAN+L F IYDLELT+V++NSE R +++ TA+RSI+++EDIDCS EL+D     
Sbjct: 316 SSLIAAMANHLNFDIYDLELTAVNNNSELRRLLIATANRSILIVEDIDCSLELKDRTSDE 375

Query: 305 --RECDDYGD-RKGKLTLSGVLNAIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDM 364
             RE DD  D R  K+TLSG+LN IDGLWSSCGD RII+FTTN+KEKLD ALLRPGRMDM
Sbjct: 376 PPRESDDIEDPRYKKVTLSGLLNFIDGLWSSCGDERIIIFTTNYKEKLDAALLRPGRMDM 435

Query: 365 HIHMTYLTPSGFQILASNYLQIKSHDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALG 424
           HIHM+Y TPS F+ LA NYL+IK H  F +IEE I   EVTPAE+AE+LM++D  D  L 
Sbjct: 436 HIHMSYCTPSTFKALALNYLEIKEHRLFSKIEEGIEATEVTPAEVAEQLMRNDSVDKVLE 495

Query: 425 AVVEFINGKKRKRVEKDERDPQEDIEEEDPKEFKQKKG 435
            ++EF+   K K++E +E+D  +  ++E   + K K+G
Sbjct: 496 GLIEFL---KVKKIE-NEQDKAKTEKQELENKKKTKEG 526

BLAST of MC09g_new0496 vs. TAIR 10
Match: AT2G18193.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 380.9 bits (977), Expect = 1.4e-105
Identity = 210/458 (45.85%), Postives = 304/458 (66.38%), Query Frame = 0

Query: 5   RTIIHQILPQQLRHVTSSKFNAIFD-FGILSSQKLVYIIGESNGVTTNELYRATETYLHT 64
           R+++H  +P++LR    S F+++ D F    S+ L  II E+ G+  N+++ A E YL +
Sbjct: 29  RSMLHDFVPEKLR----SYFSSLLDRFFTPKSKYLTVIIDENFGLNRNQVFDAAEMYLRS 88

Query: 65  KIPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQKPNSEKRYYQ 124
           KI    ++L   K  +QK+F+  I +GE + D F+  ++ W  +   +++K +  KRYY+
Sbjct: 89  KIGPETERLRVGKIPKQKHFTISIERGEEILDTFEESEVKWS-YVQSENEKGDKVKRYYE 148

Query: 125 MSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYS------------LGYDESSIVLR 184
           ++F KK +D V + YL +++  +  I    RVVKLYS             G +   I L 
Sbjct: 149 LTFEKKLRDKVLNSYLTHVVAESEEIKRNLRVVKLYSRDVYASDDDDGMAGGNWGCINLE 208

Query: 185 NTCSFETLAMDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGKSSLVLAM 244
           +  +F+TLAMDP  KK+++DDL+RF++RK+FY+R+G+AWKRGY+LYGPPGTGKSSL+ AM
Sbjct: 209 HPSTFDTLAMDPNAKKKIIDDLERFLKRKEFYKRVGKAWKRGYLLYGPPGTGKSSLIAAM 268

Query: 245 ANYLKFSIYDLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDRECDDYGDR--K 304
           ANYLKF ++DLEL+S+  N E + ++L T +RSI+VIEDIDC+ E+ DRE ++  D   K
Sbjct: 269 ANYLKFDVFDLELSSIYDNGELKRVLLSTTNRSILVIEDIDCNAEVRDREAENQEDEQIK 328

Query: 305 GKLTLSGVLNAIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMTYLTPSGFQ 364
           GK+TLSG+LN IDGLWSS GD RIIVFTTNHKE+LDPALLRPGRMD+HI+M+Y T  GF+
Sbjct: 329 GKVTLSGILNFIDGLWSSFGDERIIVFTTNHKERLDPALLRPGRMDVHINMSYCTGLGFR 388

Query: 365 ILASNYLQIK--SHDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGKKR 424
            L SNYL +   +H   +EIE L+   EVTPAE+AEELM+ DD DV L  V+ F+  +K 
Sbjct: 389 TLVSNYLGLDGLNHPLCEEIEALVDSTEVTPAELAEELMQDDDTDVVLRGVISFVEKRKV 448

Query: 425 KRVEKDERDPQEDIEEEDPKE-------FKQKKGNTRR 439
           +R +  +        ++D K+        K+KKG  ++
Sbjct: 449 ERSKTKKEVSICKATDDDEKQNGSLGCVKKKKKGGKQK 481

BLAST of MC09g_new0496 vs. TAIR 10
Match: AT2G18190.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 371.7 bits (953), Expect = 8.4e-103
Identity = 198/421 (47.03%), Postives = 278/421 (66.03%), Query Frame = 0

Query: 5   RTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETYLHTK 64
           R++ +  +P++LR   +   N  F      S+ L  +I E  G   N+++ A E YL  K
Sbjct: 30  RSLFNDEVPERLRSYITDLLNRFF---TPKSKNLTMVIDEIIGFKRNQVFDAAEVYLRNK 89

Query: 65  IPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQKPNSEKRYYQM 124
           I     +L   K  +QK+F+  I KGE + D F+  ++ W  +   +++    EKRYY++
Sbjct: 90  IGPETARLRVGKLPKQKHFTIYIEKGEEILDTFENSELRW-TYVESENEASQKEKRYYEL 149

Query: 125 SFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYS------------LGYDESSIVLRN 184
           +F KK +D V + YL +++  +       R VKLYS             G     I L +
Sbjct: 150 TFEKKLRDKVMNSYLSHVVAESEETKRDLRAVKLYSRDVRASKDDDGMAGAGWGCINLEH 209

Query: 185 TCSFETLAMDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGTGKSSLVLAMA 244
             +FETLAMDP  KK+++DD++RF++R++FY+R+G+AWKRGY+LYGPPGTGKSSL+ AMA
Sbjct: 210 PSTFETLAMDPGAKKKIIDDMERFLKRREFYKRVGKAWKRGYLLYGPPGTGKSSLIAAMA 269

Query: 245 NYLKFSIYDLELTSVDSNSEFREMMLCTADRSIIVIEDIDCST-ELEDRECDDYGDRK-- 304
           NYLKF ++DLEL+S+  N++ + ++L T +RSI+VIEDIDCS+ E+ DRE D+Y + +  
Sbjct: 270 NYLKFDVFDLELSSIYENAQLKSILLSTTNRSILVIEDIDCSSAEVVDREADEYQEYEEG 329

Query: 305 --GKLTLSGVLNAIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMHIHMTYLTPSG 364
             G++TLSG+LN +DGLWSS GD RIIVFTTNHKE+LDPALLRPGRMDMHI+M+Y T  G
Sbjct: 330 YYGRVTLSGLLNFVDGLWSSFGDERIIVFTTNHKERLDPALLRPGRMDMHINMSYCTGLG 389

Query: 365 FQILASNYLQI--KSHDRFKEIEELIMEVEVTPAEIAEELMKSDDADVALGAVVEFINGK 407
           F+ L SNYL +   +H   +EIE LI   EVTPAE+AEELM+ DD DV L  VV F+  +
Sbjct: 390 FRTLVSNYLGLGGLNHPLCEEIEALIDSTEVTPAELAEELMQEDDTDVVLRGVVSFVENR 446

BLAST of MC09g_new0496 vs. TAIR 10
Match: AT5G17760.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 368.6 bits (945), Expect = 7.1e-102
Identity = 205/468 (43.80%), Postives = 298/468 (63.68%), Query Frame = 0

Query: 4   IRTIIHQILPQQLRHVTSSKFNAIFDFGILSSQKLVYIIGESNGVTTNELYRATETYLHT 63
           IR++ H+++P  L+        ++F     SS  L   I + N    NE+YRA +TYL T
Sbjct: 27  IRSMAHELIPAPLQDFIYRTLRSLFFRS--SSSTLTLTIDDDNMGMNNEIYRAAQTYLST 86

Query: 64  KIPLSVKQLEASKAKEQKNFSFKISKGESLTDEFQGIQITWELHSIQKDQK--------- 123
           KI     +L  SK  + K+ +  +S GE + D ++ +Q+ W   +   D+K         
Sbjct: 87  KISPDAVRLRISKGHKDKHVNLYLSDGEIVNDVYEDVQLVWRFVTDGGDKKGGGGGVGGR 146

Query: 124 -----------PNSEKRYYQMSFLKKHKDFVDDVYLPYIMNRANAIDEGNRVVKLYSL-G 183
                       + +  Y+++SF KKHKD + + Y+PYI ++A  I +  R++ L+SL  
Sbjct: 147 GGGGGRRGGMDDDGKSEYFELSFDKKHKDLILNSYVPYIESKAKEIRDERRILMLHSLNS 206

Query: 184 YDESSIVLRNTCSFETLAMDPEKKKEVMDDLDRFVRRKDFYRRLGRAWKRGYILYGPPGT 243
               S++L +  +FET+AM+ + K++V++DLDRF+RRK+FY+R+G+AWKRGY+LYGPPGT
Sbjct: 207 LRWESVILEHPSTFETMAMEDDLKRDVIEDLDRFIRRKEFYKRVGKAWKRGYLLYGPPGT 266

Query: 244 GKSSLVLAMANYLKFSIYDLELTSVDSNSEFREMMLCTADRSIIVIEDIDCSTELEDR-- 303
           GKSSLV AMANYLKF +YDL+L SV  +S+ R ++L T +RSI+VIEDIDC+ +L +R  
Sbjct: 267 GKSSLVAAMANYLKFDVYDLQLASVMRDSDLRRLLLATRNRSILVIEDIDCAVDLPNRIE 326

Query: 304 ---ECDDYGDRKGKLTLSGVLNAIDGLWSSCGDARIIVFTTNHKEKLDPALLRPGRMDMH 363
              E  + G+ +G LTLSG+LN IDGLWSSCGD RII+FTTNHK++LDPALLRPGRMDMH
Sbjct: 327 QPVEGKNRGESQGPLTLSGLLNFIDGLWSSCGDERIIIFTTNHKDRLDPALLRPGRMDMH 386

Query: 364 IHMTYLTPSGFQILASNYLQIKS----HDRFKEIEELIMEVEVTPAEIAEELMKSDDADV 423
           I+M + +  GF+ LASNYL +      H  F EIE LI    +TPA++AEELMKS+DADV
Sbjct: 387 IYMGHCSFQGFKTLASNYLGLSDAAMPHRLFPEIERLIDGEVMTPAQVAEELMKSEDADV 446

Query: 424 ALGAVVEFINGKKRKRVEKDE---RDPQEDIEEEDPKEFKQKKGNTRR 439
           AL  +V  +   + K  E +    +  +  +E E+ +     +G+ R+
Sbjct: 447 ALEGLVNVLEKMRLKSKESNPVMMKQKESRLEMEEMRLKSDTEGSPRK 492

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q147F91.5e-10947.73AAA-ATPase At3g50940 OS=Arabidopsis thaliana OX=3702 GN=At3g50940 PE=2 SV=1[more]
Q8VZG22.2e-10846.94Protein HYPER-SENSITIVITY-RELATED 4 OS=Arabidopsis thaliana OX=3702 GN=HSR4 PE=2... [more]
Q8GW961.9e-10445.85AAA-ATPase At2g18193 OS=Arabidopsis thaliana OX=3702 GN=At2g18193 PE=2 SV=1[more]
F4IQG21.2e-10147.03AAA-ATPase At2g18190 OS=Arabidopsis thaliana OX=3702 GN=At2g18190 PE=2 SV=1[more]
Q9FN751.0e-10043.80AAA-ATPase At5g17760 OS=Arabidopsis thaliana OX=3702 GN=At5g17760 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022151910.10.0100.00AAA-ATPase At3g50940-like [Momordica charantia][more]
XP_022151927.12.53e-20068.38AAA-ATPase At3g50940-like [Momordica charantia][more]
XP_038892960.12.69e-18664.49AAA-ATPase At3g50940-like [Benincasa hispida][more]
KAB1213001.15.72e-15654.25putative mitochondrial chaperone bcs1 [Morella rubra][more]
XP_028113418.14.34e-15454.12AAA-ATPase At3g50940-like [Camellia sinensis][more]
Match NameE-valueIdentityDescription
A0A6J1DCI10.0100.00AAA-ATPase At3g50940-like OS=Momordica charantia OX=3673 GN=LOC111019763 PE=3 SV... [more]
A0A6J1DET21.23e-20068.38AAA-ATPase At3g50940-like OS=Momordica charantia OX=3673 GN=LOC111019781 PE=3 SV... [more]
A0A7N2L9F51.30e-15857.52AAA domain-containing protein OS=Quercus lobata OX=97700 PE=3 SV=1[more]
A0A6A1VJH32.77e-15654.25Putative mitochondrial chaperone bcs1 OS=Morella rubra OX=262757 GN=CJ030_MR5G02... [more]
B9RMH67.20e-15456.21ATP binding protein, putative OS=Ricinus communis OX=3988 GN=RCOM_1080880 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT3G50940.11.1e-11047.73P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G50930.11.6e-10946.94cytochrome BC1 synthesis [more]
AT2G18193.11.4e-10545.85P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G18190.18.4e-10347.03P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G17760.17.1e-10243.80P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003593AAA+ ATPase domainSMARTSM00382AAA_5coord: 210..344
e-value: 6.9E-6
score: 35.6
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 215..341
e-value: 2.4E-16
score: 60.4
NoneNo IPR availableGENE3D6.10.280.40coord: 339..407
e-value: 4.7E-19
score: 70.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 406..452
NoneNo IPR availablePANTHERPTHR23070BCS1 AAA-TYPE ATPASEcoord: 3..437
NoneNo IPR availablePANTHERPTHR23070:SF154SUBFAMILY NOT NAMEDcoord: 3..437
NoneNo IPR availableCDDcd00009AAAcoord: 211..342
e-value: 1.63741E-10
score: 57.1559
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 164..338
e-value: 9.1E-42
score: 144.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 88..391
IPR025753AAA-type ATPase, N-terminal domainPFAMPF14363AAA_assoccoord: 12..107
e-value: 4.3E-20
score: 71.9
IPR003960ATPase, AAA-type, conserved sitePROSITEPS00674AAAcoord: 312..330

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g_new0496.1MC09g_new0496.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity