MC09g_new0210 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g_new0210
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
LocationMC09: 10308603 .. 10310683 (-)
RNA-Seq ExpressionMC09g_new0210
SyntenyMC09g_new0210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TGACCATAAACTTCAATTCCGCTCTACTCCATGTAACTTCTTAGGATATAGCAATATACACAAAGGGTATAAATGTCTCTCTACCACTGGCCGCATCTACATTTCCAGAAATGTCACCTTTGATGAAACTAATTTCCCTTTCTCTGCGACTACGTCTGTTCCTCAACCTTTTCAATCCACCACAATCCCTTCCACTTTATCTCATGTTCCATATACTACTCCTTTGTCTTCCCCTGATCCCACAAATCCTGCCCCCACCCCTCTACCTCTCACTCATCCCACATCCCCACTATCTTTACCCAACAGCACCTCACCTATTCAACTTCCCTCTTCACCCATTCAATCAAACCAACCTATCTCACCCCCTTCTTCTACTTCCACACCAGAGGCATCTCCACCAGTGTTTATACCTTCTTCTCCTAATACTATATTACAGAATCCTCATCCTATGACTACACGTGCTAAAAATGGCATTTTTAGGCCAAAAATTTATCTTGCAGACTATAAAGAAGTCGAGCCTTCCTCGGCAAAAGAGGCTCTCAAATATCCCCATTGGAAAGCAGCTATGGAAGAAGAGTATAATGCTTTGTTGAAAAATGGTACATGGACACTGACTTCTCCCCCTCCTGATAAGAAGATCGTCGGTTGCAAATGGGTCTTCAGAATAAAGCGTAATACAGATGGATCAGTCGCTCGGTATAAAGCCCATCTTGTTGCAAAAGGTTTTCATCAAACAGCTGCCATTGACTATCAGGAAACATTTAGTCCTGTGGTGAAACATGTGACAATCCGCGTTCTCCTTACTTTGGCTATTACATTTGGTTGGGACTTACGCCAAATAGACATCAACAATGCCTTCCTCCATGGCATTCTCCATGACTCAGTTCATATGGAACAACCACCTGGTTTCATAGCCCCTGGTTCTGGTACTATGGTTTGTAAACTTCACAAAGCTTTATATGGCCTGAAGCAAGCTCCCCCAGCATGGTTTGAAAGGCTTAGCTCCTATCTTGTTTCACTCGGTTTTAGACATTCACGGGCTGATCCGTCACTGATGTATCAATTCAAAGATGGCAGGTGTTGCTATGTTCTCATCTATGTCGATGACATCGTCATTACTGGGAACTCGCCTACTGCCATTCAGCATCTTATTAAATCTTTACATGGTAAATTCTCTCTTAAGGATTTGGGTAAATTGGGATATTTTCTTAGCATTGAAGTATCATATCCATCCAAGGGCGAAATCTATCTATCCCAGGCAAAGTATATTACTAATTTGTTGCAGAAAGCAAATATGTTAGAGTCCAACAGCATTGCCACTCCTATGATCAGTGGCTCCATAACCTCTGCTTTTCAAGGTGAATCTCTTCCGGATCCTTTCCAATACAGAAGCGTTGTCGGTGCACTGCAGTACGTCACCTTGACTCGACCAAAACTTGCCTATTGTGTCAATAAGGTGTGCCAATTTATGCACAACCTAACTCACATTCACTGACAAATGGTAAAACGGATACTTCGTTACCTAAAGGGCACTTATGACCATGGTTTACTGCTGTCCCACTCGTCTGATCTACGTCTTCAGGCTTTCTCTGATGCCGATTGGGCATCTGACCCCGATGACCGCAAGTCCACTTCAGGGTTCTGCATCTTCTTTGGTGGAAATCCAATATCCTGGGGTTCTAAGAAACAACCTGTTATCTCACGATCCAGTACTGAGGCTGAATATAGGTCACTTGCTCTATCTGCTTCTGAACTCATCTGGCTTCGCACACTATTTGCTGAGCTTCGGTTTCCTCTGCTTCATCCACCAATTTTGTGGTGTGATAATCTTAGTGCCGTACATCTCAGTGCAAATCCAATCCTCCATTCTCGCACTAAACACGTTGAAATTGACATTTACTTTGTCCGCGATATGGTTCTCTCTAATCAACTTCGTGTTCAACATCTTCCCTCGTCTGCCCAAATTGCGGACATACTGACCAAGCCACTGTCCTCTGCCTCTTTTCATCTTCTGAAGTCCAAGCTCAATGTTCGGCCTGCCTCCTCCATTGGCTTGAGGGGGGGTGTTAAGACTAATCACT

mRNA sequence

ATGACTACACGTGCTAAAAATGGCATTTTTAGGCCAAAAATTTATCTTGCAGACTATAAAGAAGTCGAGCCTTCCTCGGCAAAAGAGGCTCTCAAATATCCCCATTGGAAAGCAGCTATGGAAGAAGAGTATAATGCTTTGTTGAAAAATGGTACATGGACACTGACTTCTCCCCCTCCTGATAAGAAGATCGTCGGTTGCAAATGGGTCTTCAGAATAAAGCGTAATACAGATGGATCAGTCGCTCGGTATAAAGCCCATCTTGTTGCAAAAGGTTTTCATCAAACAGCTGCCATTGACTATCAGGAAACATTTAGTCCTGTGGTGAAACATGTGACAATCCGCGTTCTCCTTACTTTGGCTATTACATTTGGTTGGGACTTACGCCAAATAGACATCAACAATGCCTTCCTCCATGGCATTCTCCATGACTCAGTTCATATGGAACAACCACCTGGTTTCATAGCCCCTGGTTCTGGTACTATGGTTTGTAAACTTCACAAAGCTTTATATGGCCTGAAGCAAGCTCCCCCAGCATGGTTTGAAAGGCTTAGCTCCTATCTTGTTTCACTCGGTTTTAGACATTCACGGGCTGATCCGTCACTGATGTATCAATTCAAAGATGGCAGGTGTTGCTATGTTCTCATCTATGTCGATGACATCGTCATTACTGGGAACTCGCCTACTGCCATTCAGCATCTTATTAAATCTTTACATGGTAAATTCTCTCTTAAGGATTTGGGTAAATTGGGATATTTTCTTAGCATTGAAGTATCATATCCATCCAAGGGCGAAATCTATCTATCCCAGGCAAAGTATATTACTAATTTGTTGCAGAAAGCAAATATGTTAGAGTCCAACAGCATTGCCACTCCTATGATCAGTGGCTCCATAACCTCTGCTTTTCAAGGTGAATCTCTTCCGGATCCTTTCCAATACAGAAGCGTTGTCGGTGCACTGCAGTACGTCACCTTGACTCGACCAAAACTTGCCTATTGTGTCAATAAGGTGTGCCAATTTATGCACAACCTAACTCACATTCACTGA

Coding sequence (CDS)

ATGACTACACGTGCTAAAAATGGCATTTTTAGGCCAAAAATTTATCTTGCAGACTATAAAGAAGTCGAGCCTTCCTCGGCAAAAGAGGCTCTCAAATATCCCCATTGGAAAGCAGCTATGGAAGAAGAGTATAATGCTTTGTTGAAAAATGGTACATGGACACTGACTTCTCCCCCTCCTGATAAGAAGATCGTCGGTTGCAAATGGGTCTTCAGAATAAAGCGTAATACAGATGGATCAGTCGCTCGGTATAAAGCCCATCTTGTTGCAAAAGGTTTTCATCAAACAGCTGCCATTGACTATCAGGAAACATTTAGTCCTGTGGTGAAACATGTGACAATCCGCGTTCTCCTTACTTTGGCTATTACATTTGGTTGGGACTTACGCCAAATAGACATCAACAATGCCTTCCTCCATGGCATTCTCCATGACTCAGTTCATATGGAACAACCACCTGGTTTCATAGCCCCTGGTTCTGGTACTATGGTTTGTAAACTTCACAAAGCTTTATATGGCCTGAAGCAAGCTCCCCCAGCATGGTTTGAAAGGCTTAGCTCCTATCTTGTTTCACTCGGTTTTAGACATTCACGGGCTGATCCGTCACTGATGTATCAATTCAAAGATGGCAGGTGTTGCTATGTTCTCATCTATGTCGATGACATCGTCATTACTGGGAACTCGCCTACTGCCATTCAGCATCTTATTAAATCTTTACATGGTAAATTCTCTCTTAAGGATTTGGGTAAATTGGGATATTTTCTTAGCATTGAAGTATCATATCCATCCAAGGGCGAAATCTATCTATCCCAGGCAAAGTATATTACTAATTTGTTGCAGAAAGCAAATATGTTAGAGTCCAACAGCATTGCCACTCCTATGATCAGTGGCTCCATAACCTCTGCTTTTCAAGGTGAATCTCTTCCGGATCCTTTCCAATACAGAAGCGTTGTCGGTGCACTGCAGTACGTCACCTTGACTCGACCAAAACTTGCCTATTGTGTCAATAAGGTGTGCCAATTTATGCACAACCTAACTCACATTCACTGA

Protein sequence

MTTRAKNGIFRPKIYLADYKEVEPSSAKEALKYPHWKAAMEEEYNALLKNGTWTLTSPPPDKKIVGCKWVFRIKRNTDGSVARYKAHLVAKGFHQTAAIDYQETFSPVVKHVTIRVLLTLAITFGWDLRQIDINNAFLHGILHDSVHMEQPPGFIAPGSGTMVCKLHKALYGLKQAPPAWFERLSSYLVSLGFRHSRADPSLMYQFKDGRCCYVLIYVDDIVITGNSPTAIQHLIKSLHGKFSLKDLGKLGYFLSIEVSYPSKGEIYLSQAKYITNLLQKANMLESNSIATPMISGSITSAFQGESLPDPFQYRSVVGALQYVTLTRPKLAYCVNKVCQFMHNLTHIH
Homology
BLAST of MC09g_new0210 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 6.1e-90
Identity = 168/353 (47.59%), Postives = 228/353 (64.59%), Query Frame = 0

Query: 1    MTTRAKNGIFRP----KIYLADYKEVEPSSAKEALKYPHWKAAMEEEYNALLKNGTWTLT 60
            M TRAK GI +P     + ++   E EP +A +ALK   W+ AM  E NA + N TW L 
Sbjct: 929  MGTRAKAGIIKPNPKYSLAVSLAAESEPRTAIQALKDERWRNAMGSEINAQIGNHTWDLV 988

Query: 61   SPPPDK-KIVGCKWVFRIKRNTDGSVARYKAHLVAKGFHQTAAIDYQETFSPVVKHVTIR 120
             PPP    IVGC+W+F  K N+DGS+ RYKA LVAKG++Q   +DY ETFSPV+K  +IR
Sbjct: 989  PPPPSHVTIVGCRWIFTKKYNSDGSLNRYKARLVAKGYNQRPGLDYAETFSPVIKSTSIR 1048

Query: 121  VLLTLAITFGWDLRQIDINNAFLHGILHDSVHMEQPPGFIAPGSGTMVCKLHKALYGLKQ 180
            ++L +A+   W +RQ+D+NNAFL G L D V+M QPPGFI       VCKL KALYGLKQ
Sbjct: 1049 IVLGVAVDRSWPIRQLDVNNAFLQGTLTDDVYMSQPPGFIDKDRPNYVCKLRKALYGLKQ 1108

Query: 181  APPAWFERLSSYLVSLGFRHSRADPSLMYQFKDGRCCYVLIYVDDIVITGNSPTAIQHLI 240
            AP AW+  L +YL+++GF +S +D SL    +     Y+L+YVDDI+ITGN PT + + +
Sbjct: 1109 APRAWYVELRNYLLTIGFVNSVSDTSLFVLQRGKSIVYMLVYVDDILITGNDPTLLHNTL 1168

Query: 241  KSLHGKFSLKDLGKLGYFLSIEVSYPSKGEIYLSQAKYITNLLQKANMLESNSIATPMIS 300
             +L  +FS+KD  +L YFL IE      G ++LSQ +YI +LL + NM+ +  + TPM  
Sbjct: 1169 DNLSQRFSVKDHEELHYFLGIEAKRVPTG-LHLSQRRYILDLLARTNMITAKPVTTPMAP 1228

Query: 301  GSITSAFQGESLPDPFQYRSVVGALQYVTLTRPKLAYCVNKVCQFMHNLTHIH 349
                S + G  L DP +YR +VG+LQY+  TRP ++Y VN++ QFMH  T  H
Sbjct: 1229 SPKLSLYSGTKLTDPTEYRGIVGSLQYLAFTRPDISYAVNRLSQFMHMPTEEH 1280

BLAST of MC09g_new0210 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 2.8e-87
Identity = 162/353 (45.89%), Postives = 227/353 (64.31%), Query Frame = 0

Query: 1    MTTRAKNGIFRPKIYLADYKEV----EPSSAKEALKYPHWKAAMEEEYNALLKNGTWTLT 60
            M TRAK+GI +P    +    +    EP +A +A+K   W+ AM  E NA + N TW L 
Sbjct: 912  MATRAKDGIRKPNQKYSYATSLAANSEPRTAIQAMKDDRWRQAMGSEINAQIGNHTWDLV 971

Query: 61   SPPPDK-KIVGCKWVFRIKRNTDGSVARYKAHLVAKGFHQTAAIDYQETFSPVVKHVTIR 120
             PPP    IVGC+W+F  K N+DGS+ RYKA LVAKG++Q   +DY ETFSPV+K  +IR
Sbjct: 972  PPPPPSVTIVGCRWIFTKKFNSDGSLNRYKARLVAKGYNQRPGLDYAETFSPVIKSTSIR 1031

Query: 121  VLLTLAITFGWDLRQIDINNAFLHGILHDSVHMEQPPGFIAPGSGTMVCKLHKALYGLKQ 180
            ++L +A+   W +RQ+D+NNAFL G L D V+M QPPGF+       VC+L KA+YGLKQ
Sbjct: 1032 IVLGVAVDRSWPIRQLDVNNAFLQGTLTDEVYMSQPPGFVDKDRPDYVCRLRKAIYGLKQ 1091

Query: 181  APPAWFERLSSYLVSLGFRHSRADPSLMYQFKDGRCCYVLIYVDDIVITGNSPTAIQHLI 240
            AP AW+  L +YL+++GF +S +D SL    +     Y+L+YVDDI+ITGN    ++H +
Sbjct: 1092 APRAWYVELRTYLLTVGFVNSISDTSLFVLQRGRSIIYMLVYVDDILITGNDTVLLKHTL 1151

Query: 241  KSLHGKFSLKDLGKLGYFLSIEVSYPSKGEIYLSQAKYITNLLQKANMLESNSIATPMIS 300
             +L  +FS+K+   L YFL IE     +G ++LSQ +Y  +LL + NML +  +ATPM +
Sbjct: 1152 DALSQRFSVKEHEDLHYFLGIEAKRVPQG-LHLSQRRYTLDLLARTNMLTAKPVATPMAT 1211

Query: 301  GSITSAFQGESLPDPFQYRSVVGALQYVTLTRPKLAYCVNKVCQFMHNLTHIH 349
                +   G  LPDP +YR +VG+LQY+  TRP L+Y VN++ Q+MH  T  H
Sbjct: 1212 SPKLTLHSGTKLPDPTEYRGIVGSLQYLAFTRPDLSYAVNRLSQYMHMPTDDH 1263

BLAST of MC09g_new0210 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 5.0e-60
Identity = 130/335 (38.81%), Postives = 191/335 (57.01%), Query Frame = 0

Query: 23   EPSSAKEALKYP---HWKAAMEEEYNALLKNGTWTLTSPPPDKKIVGCKWVFRIKRNTDG 82
            EP S KE L +P       AM+EE  +L KNGT+ L   P  K+ + CKWVF++K++ D 
Sbjct: 810  EPESLKEVLSHPEKNQLMKAMQEEMESLQKNGTYKLVELPKGKRPLKCKWVFKLKKDGDC 869

Query: 83   SVARYKAHLVAKGFHQTAAIDYQETFSPVVKHVTIRVLLTLAITFGWDLRQIDINNAFLH 142
             + RYKA LV KGF Q   ID+ E FSPVVK  +IR +L+LA +   ++ Q+D+  AFLH
Sbjct: 870  KLVRYKARLVVKGFEQKKGIDFDEIFSPVVKMTSIRTILSLAASLDLEVEQLDVKTAFLH 929

Query: 143  GILHDSVHMEQPPGFIAPGSGTMVCKLHKALYGLKQAPPAWFERLSSYLVSLGFRHSRAD 202
            G L + ++MEQP GF   G   MVCKL+K+LYGLKQAP  W+ +  S++ S  +  + +D
Sbjct: 930  GDLEEEIYMEQPEGFEVAGKKHMVCKLNKSLYGLKQAPRQWYMKFDSFMKSQTYLKTYSD 989

Query: 203  PSLMY-QFKDGRCCYVLIYVDDIVITGNSPTAIQHLIKSLHGKFSLKDLGKLGYFLSIE- 262
            P + + +F +     +L+YVDD++I G     I  L   L   F +KDLG     L ++ 
Sbjct: 990  PCVYFKRFSENNFIILLLYVDDMLIVGKDKGLIAKLKGDLSKSFDMKDLGPAQQILGMKI 1049

Query: 263  VSYPSKGEIYLSQAKYITNLLQKANMLESNSIATPMISG--------SITSAFQGESLPD 322
            V   +  +++LSQ KYI  +L++ NM  +  ++TP+             T   +G     
Sbjct: 1050 VRERTSRKLWLSQEKYIERVLERFNMKNAKPVSTPLAGHLKLSKKMCPTTVEEKGNMAKV 1109

Query: 323  PFQYRSVVGALQY-VTLTRPKLAYCVNKVCQFMHN 344
            P  Y S VG+L Y +  TRP +A+ V  V +F+ N
Sbjct: 1110 P--YSSAVGSLMYAMVCTRPDIAHAVGVVSRFLEN 1142

BLAST of MC09g_new0210 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 203.4 bits (516), Expect = 4.3e-51
Identity = 116/311 (37.30%), Postives = 175/311 (56.27%), Query Frame = 0

Query: 36   WKAAMEEEYNALLKNGTWTLTSPPPDKKIVGCKWVFRIKRNTDGSVARYKAHLVAKGFHQ 95
            W+ A+  E NA   N TWT+T  P +K IV  +WVF +K N  G+  RYKA LVA+GF Q
Sbjct: 906  WEEAINTELNAHKINNTWTITKRPENKNIVDSRWVFSVKYNELGNPIRYKARLVARGFTQ 965

Query: 96   TAAIDYQETFSPVVKHVTIRVLLTLAITFGWDLRQIDINNAFLHGILHDSVHMEQPPGFI 155
               IDY+ETF+PV +  + R +L+L I +   + Q+D+  AFL+G L + ++M  P G  
Sbjct: 966  KYQIDYEETFAPVARISSFRFILSLVIQYNLKVHQMDVKTAFLNGTLKEEIYMRLPQGIS 1025

Query: 156  APGSGTMVCKLHKALYGLKQAPPAWFERLSSYLVSLGFRHSRADPSLMYQFKD--GRCCY 215
               +   VCKL+KA+YGLKQA   WFE     L    F +S  D  +    K       Y
Sbjct: 1026 C--NSDNVCKLNKAIYGLKQAARCWFEVFEQALKECEFVNSSVDRCIYILDKGNINENIY 1085

Query: 216  VLIYVDDIVITGNSPTAIQHLIKSLHGKFSLKDLGKLGYFLSIEVSYPSKGEIYLSQAKY 275
            VL+YVDD+VI     T + +  + L  KF + DL ++ +F+ I +    + +IYLSQ+ Y
Sbjct: 1086 VLLYVDDVVIATGDMTRMNNFKRYLMEKFRMTDLNEIKHFIGIRIEM-QEDKIYLSQSAY 1145

Query: 276  ITNLLQKANMLESNSIATPM---ISGSITSAFQGESLPDPFQYRSVVGALQYVTL-TRPK 335
            +  +L K NM   N+++TP+   I+  + ++ +  + P     RS++G L Y+ L TRP 
Sbjct: 1146 VKKILSKFNMENCNAVSTPLPSKINYELLNSDEDCNTP----CRSLIGCLMYIMLCTRPD 1205

Query: 336  LAYCVNKVCQF 341
            L   VN + ++
Sbjct: 1206 LTTAVNILSRY 1209

BLAST of MC09g_new0210 vs. ExPASy Swiss-Prot
Match: P25600 (Putative transposon Ty5-1 protein YCL074W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY5A PE=5 SV=2)

HSP 1 Score: 120.6 bits (301), Expect = 3.6e-26
Identity = 64/219 (29.22%), Postives = 113/219 (51.60%), Query Frame = 0

Query: 131 IDINNAFLHGILHDSVHMEQPPGFIAPGSGTMVCKLHKALYGLKQAPPAWFERLSSYLVS 190
           +D++ AFL+  + + ++++QPPGF+   +   V +L+  +YGLKQAP  W E +++ L  
Sbjct: 1   MDVDTAFLNSTMDEPIYVKQPPGFVNERNPDYVWELYGGMYGLKQAPLLWNEHINNTLKK 60

Query: 191 LGFRHSRADPSLMYQFKDGRCCYVLIYVDDIVITGNSPTAIQHLIKSLHGKFSLKDLGKL 250
           +GF     +  L ++       Y+ +YVDD+++   SP     + + L   +S+KDLGK+
Sbjct: 61  IGFCRHEGEHGLYFRSTSDGPIYIAVYVDDLLVAAPSPKIYDRVKQELTKLYSMKDLGKV 120

Query: 251 GYFLSIEVSYPSKGEIYLSQAKYITNLLQKANMLESNSIATPMISGSITSAFQGESLPDP 310
             FL + +   S G+I LS   YI     ++ +       TP+ +           L D 
Sbjct: 121 DKFLGLNIHQSSNGDITLSLQDYIAKAASESEINTFKLTQTPLCNSKPLFETTSPHLKDI 180

Query: 311 FQYRSVVGALQYVTLT-RPKLAYCVNKVCQFMHNLTHIH 349
             Y+S+VG L +   T RP ++Y V+ + +F+     IH
Sbjct: 181 TPYQSIVGQLLFCANTGRPDISYPVSLLSRFLREPRAIH 219

BLAST of MC09g_new0210 vs. NCBI nr
Match: KYP48058.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cajanus cajan])

HSP 1 Score: 395 bits (1014), Expect = 5.60e-131
Identity = 195/341 (57.18%), Postives = 248/341 (72.73%), Query Frame = 0

Query: 1   MTTRAKNGIFRPKIYLADYKEVEPSSAKEALKYPHWKAAMEEEYNALLKNGTWTLTSPPP 60
           M TR+K+GI +PK       + EP + K+ALK P W  AM++EY+ALLKN TWTL   PP
Sbjct: 1   MQTRSKSGIHKPKYPSVLLAQSEPKNVKQALKDPKWLEAMKQEYSALLKNNTWTLVQLPP 60

Query: 61  DKKIVGCKWVFRIKRNTDGSVARYKAHLVAKGFHQTAAIDYQETFSPVVKHVTIRVLLTL 120
           D+  +GCKWVFRIK N+DGSV RYKA LVAKGF Q    D+ ETFSP++K VTIR++LTL
Sbjct: 61  DRNAIGCKWVFRIKENSDGSVNRYKARLVAKGFLQQPGFDFNETFSPIIKPVTIRLILTL 120

Query: 121 AITFGWDLRQIDINNAFLHGILHDSVHMEQPPGFIAPGSGTMVCKLHKALYGLKQAPPAW 180
           AI+  WD+ Q+D+NNAFL+G L+++V+M+QPPGF  P S T+VCKL+KALYGLKQAP  W
Sbjct: 121 AISNHWDIHQLDVNNAFLNGSLNETVYMQQPPGFEVPDS-TLVCKLNKALYGLKQAPRQW 180

Query: 181 FERLSSYLVSLGFRHSRADPSLMYQFKDGRCCYVLIYVDDIVITGNSPTAIQHLIKSLHG 240
           FERL   L+  GF+ S+ DPSL          Y+L+YVDDI+ITGNS    Q LI  L+ 
Sbjct: 181 FERLQYTLLQFGFKASKCDPSLFTYHMQSNIVYLLVYVDDIIITGNSFQFTQQLIDQLNS 240

Query: 241 KFSLKDLGKLGYFLSIEVSYPSKGEIYLSQAKYITNLLQKANMLESNSIATPMISGSITS 300
            FSLK LGKL YFL IEV +   G + L+Q+KYI +LLQ+ NMLE+N+I++PM+S    S
Sbjct: 241 IFSLKQLGKLDYFLGIEVRHLQNGSVLLTQSKYIRDLLQRTNMLEANAISSPMVSSCKLS 300

Query: 301 AFQGESLPDPFQYRSVVGALQYVTLTRPKLAYCVNKVCQFM 341
               ++L D   YRSVVGALQY T+TRP+L++ VNKVCQFM
Sbjct: 301 KHGSDTLADSTFYRSVVGALQYATITRPELSFAVNKVCQFM 340

BLAST of MC09g_new0210 vs. NCBI nr
Match: KYP50410.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cajanus cajan])

HSP 1 Score: 390 bits (1001), Expect = 4.98e-129
Identity = 192/344 (55.81%), Postives = 248/344 (72.09%), Query Frame = 0

Query: 1   MTTRAKNGIFRPKIY-LADYKEVEPSSAKEALKYPHWKAAMEEEYNALLKNGTWTLTSPP 60
           MTTRAK GI +P++        +EP + K+AL    W AAM++EYNALL NGTWTL S P
Sbjct: 1   MTTRAKAGIVKPRLQPTLLLTHIEPKNTKQALASDTWLAAMKQEYNALLNNGTWTLVSLP 60

Query: 61  PDKKIVGCKWVFRIKRNTDGSVARYKAHLVAKGFHQTAAIDYQETFSPVVKHVTIRVLLT 120
           P+K  +GCKWVFRIK N+DG+V +YKA LVAKGFHQ    DY ETFSPVVK VTIR++LT
Sbjct: 61  PNKTAIGCKWVFRIKENSDGTVNKYKARLVAKGFHQQFGSDYNETFSPVVKPVTIRLILT 120

Query: 121 LAITFGWDLRQIDINNAFLHGILHDSVHMEQPPGFIAPGSGTMVCKLHKALYGLKQAPPA 180
           LA+T  W ++Q+D+NNAFL+G L + V+M QPPGF +    T+VCKLHKA+YGLKQAP A
Sbjct: 121 LALTHHWSIQQLDVNNAFLNGNLQEEVYMSQPPGFESSDK-TLVCKLHKAIYGLKQAPRA 180

Query: 181 WFERLSSYLVSLGFRHSRADPSLMYQFKDGRCCYVLIYVDDIVITGNSPTAIQHLIKSLH 240
           WF++L + L  L F+ S+ DPSL     D    Y+L+YVDDI+ITGN+ + +Q++I  L+
Sbjct: 181 WFDKLKTTLFQLNFQISKCDPSLFVYSHDNNVIYILVYVDDIIITGNNSSTLQNIIAQLN 240

Query: 241 GKFSLKDLGKLGYFLSIEVSYPSKGEIYLSQAKYITNLLQKANMLESNSIATPMISGSIT 300
             FSLKDLG+L +FL IEV   + G + L+Q+KYI +LL + NM  SN I++PM+SG   
Sbjct: 241 SAFSLKDLGRLDFFLGIEVKSNTDGSLTLTQSKYIRDLLNRTNMENSNPISSPMVSGCKL 300

Query: 301 SAFQGESLPDPFQYRSVVGALQYVTLTRPKLAYCVNKVCQFMHN 343
           S    E   D   YRSVVGALQY T+TRP++++ VNKVCQFM N
Sbjct: 301 SRTGSEKFLDVTLYRSVVGALQYATITRPEISFSVNKVCQFMSN 343

BLAST of MC09g_new0210 vs. NCBI nr
Match: KAA0052371.1 (putative mitochondrial protein [Cucumis melo var. makuwa] >TYK09417.1 putative mitochondrial protein [Cucumis melo var. makuwa])

HSP 1 Score: 388 bits (997), Expect = 6.50e-129
Identity = 190/309 (61.49%), Postives = 233/309 (75.40%), Query Frame = 0

Query: 40  MEEEYNALLKNGTWTLTSPPPDKKIVGCKWVFRIKRNTDGSVARYKAHLVAKGFHQTAAI 99
           MEEE+ AL KN TW LT   P++KIVGCKWVF+IKRN+ G +ARYKA LVAKGFHQT  I
Sbjct: 1   MEEEFEALQKNDTWRLTPQNPNQKIVGCKWVFKIKRNSYGPIARYKARLVAKGFHQTPNI 60

Query: 100 DYQETFSPVVKHVTIRVLLTLAITFGWDLRQIDINNAFLHGILHDSVHMEQPPGFIAPGS 159
           DY ETFSPVVK +TI +LLT+AI  GW +RQ+D+NNAFLHG L ++V+MEQ  GF    S
Sbjct: 61  DYNETFSPVVKSITICMLLTIAIMKGWSIRQLDVNNAFLHGNLDENVYMEQIFGFEVKSS 120

Query: 160 GTMVCKLHKALYGLKQAPPAWFERLSSYLVSLGFRHSRADPSLMYQFKDGRCCYVLIYVD 219
             MVC L KALYGLKQAP AW+E LS  L SLGFR S+AD SL+ +     CCY LIYVD
Sbjct: 121 YPMVCHLKKALYGLKQAPRAWYENLSLGLHSLGFRTSKADTSLLIRVTPTSCCYALIYVD 180

Query: 220 DIVITGNSPTAIQHLIKSLHGKFSLKDLGKLGYFLSIEVSYPSKGEIYLSQAKYITNLLQ 279
           D++I G+S   +  L+ SL+ +F+LKDLGKL YFL +EVSYP+ G+++LSQ+KYIT+LLQ
Sbjct: 181 DLIIMGSSKKDVNSLVHSLNSQFALKDLGKLSYFLGVEVSYPTNGDLFLSQSKYITDLLQ 240

Query: 280 KANMLESNSIATPMISGSITSAFQGESLPDPFQYRSVVGALQYVTLTRPKLAYCVNKVCQ 339
           +  ML++  I+TPM+SG + SAFQGE   D    RSVVGALQY TLT P+++Y VNK CQ
Sbjct: 241 RTKMLDAKPISTPMVSGPLLSAFQGEPFHDVHLLRSVVGALQYATLTHPEISYSVNKACQ 300

Query: 340 FMHNLTHIH 348
           FMH   H H
Sbjct: 301 FMHTPKHTH 309

BLAST of MC09g_new0210 vs. NCBI nr
Match: KAG8489058.1 (hypothetical protein CXB51_017051 [Gossypium anomalum])

HSP 1 Score: 389 bits (998), Expect = 1.37e-128
Identity = 188/350 (53.71%), Postives = 253/350 (72.29%), Query Frame = 0

Query: 1   MTTRAKNGIFRPKIYLADYKEVEPSSAKEALKYPHWKAAMEEEYNALLKNGTWTLTSPPP 60
           M TR+K GIF+PK   AD  ++EPSS +EAL +P W++A++ EY+AL+ N TW L S PP
Sbjct: 1   MRTRSKAGIFKPKALCADNIDLEPSSVEEALAHPAWRSAVQAEYDALIANSTWELCSLPP 60

Query: 61  DKKIVGCKWVFRIKRNTDGSVARYKAHLVAKGFHQTAAIDYQETFSPVVKHVTIRVLLTL 120
            +K +GCKW+F+IK+N DG++   KA LVAKG  Q    D+ ETFSPVVK  TIRV+L++
Sbjct: 61  GRKAIGCKWLFKIKKNPDGTINHRKARLVAKGCSQVPGCDFTETFSPVVKPATIRVILSV 120

Query: 121 AITFGWDLRQIDINNAFLHGILHDSVHMEQPPGFIAPGSG--TMVCKLHKALYGLKQAPP 180
           A+T GW LRQ+D+NNAFL+G L + V+M+QPPGF+  GS   T+VC L KALYGL+QAP 
Sbjct: 121 AVTKGWPLRQVDVNNAFLNGDLVNDVYMQQPPGFVQHGSSGETLVCHLTKALYGLRQAPR 180

Query: 181 AWFERLSSYLVSLGFRHSRADPSLMYQFKDGRCCYVLIYVDDIVITGNSPTAIQHLIKSL 240
           AWF++L  +LVS GF  S++D SL  +       YVL+YVDDIVITG+S   I   ++ L
Sbjct: 181 AWFDKLKQFLVSTGFTLSKSDASLFVRSTFDNTLYVLVYVDDIVITGSSSDEIDCFVRQL 240

Query: 241 HGKFSLKDLGKLGYFLSIEVSYPSKGEIYLSQAKYITNLLQKANMLESNSIATPMISGSI 300
           H KF+LKDLG L YFL ++V+  S G ++L Q KYI  LL +++M  + S+ TPM+S S+
Sbjct: 241 HNKFALKDLGDLQYFLGLKVTRSSSGTLHLCQRKYIRELLARSSMSTAKSVHTPMVSSSM 300

Query: 301 TSAFQGESLPDPFQYRSVVGALQYVTLTRPKLAYCVNKVCQFMHNLTHIH 348
            S  +G+ L DP +YRS+ GALQY+ LTRP +AY VN+VCQFMH  T++H
Sbjct: 301 LSKGEGDYLADPTEYRSLAGALQYIVLTRPDIAYAVNRVCQFMHAPTNLH 350

BLAST of MC09g_new0210 vs. NCBI nr
Match: MCH84623.1 (retrovirus-related Pol polyprotein from transposon TNT 1-94 [Trifolium medium])

HSP 1 Score: 387 bits (993), Expect = 2.03e-128
Identity = 185/341 (54.25%), Postives = 250/341 (73.31%), Query Frame = 0

Query: 1   MTTRAKNGIFRPKIYLADYKEVEPSSAKEALKYPHWKAAMEEEYNALLKNGTWTLTSPPP 60
           M TR K G  +PK++LA   E+EP S + AL  P WK AM++EY AL+ N TW+L   PP
Sbjct: 98  MITRGKTGSLKPKVFLA---ELEPKSVRSALSDPKWKQAMQDEYKALMDNKTWSLVPLPP 157

Query: 61  DKKIVGCKWVFRIKRNTDGSVARYKAHLVAKGFHQTAAIDYQETFSPVVKHVTIRVLLTL 120
            +K +GCKW+FR+K N DG+V ++KA LVAKGF QTA  D+ ETFSPV+K +TIR++LTL
Sbjct: 158 HRKAIGCKWIFRVKENPDGTVNKFKARLVAKGFLQTAGFDFTETFSPVIKPITIRIILTL 217

Query: 121 AITFGWDLRQIDINNAFLHGILHDSVHMEQPPGFIAPGSGTMVCKLHKALYGLKQAPPAW 180
           A+TF W ++QID+NNAFL+G+L + V+M QP GF +    ++VCKLHK+LYGLKQAP AW
Sbjct: 218 AVTFKWQVQQIDVNNAFLNGVLQEEVYMAQPSGFESSDK-SLVCKLHKSLYGLKQAPRAW 277

Query: 181 FERLSSYLVSLGFRHSRADPSLMYQFKDGRCCYVLIYVDDIVITGNSPTAIQHLIKSLHG 240
           ++RL+  L+ LGF  S+ DPSL+   ++G C YVLIY+DDI+ITG++P  I  LI  L+ 
Sbjct: 278 YDRLTQALLQLGFVKSKCDPSLLVHNQNGTCTYVLIYMDDILITGSAPHLINDLINKLNM 337

Query: 241 KFSLKDLGKLGYFLSIEVSYPSKGEIYLSQAKYITNLLQKANMLESNSIATPMISGSITS 300
            F+LK LG++ YFL IEV + S G + L+Q+KY+ +LL KA M +S  I +PM+S    S
Sbjct: 338 SFALKRLGQVDYFLGIEVHHLSSGALLLNQSKYVRDLLCKAKMEDSKPIGSPMVSSCRLS 397

Query: 301 AFQGESLPDPFQYRSVVGALQYVTLTRPKLAYCVNKVCQFM 341
            +  +++ DP  YRS+VGALQY TLTRP +A+ VNKVCQFM
Sbjct: 398 KYGTDTMTDPTLYRSIVGALQYATLTRPDIAFSVNKVCQFM 434

BLAST of MC09g_new0210 vs. ExPASy TrEMBL
Match: A0A151RZS0 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cajanus cajan OX=3821 GN=KK1_030253 PE=4 SV=1)

HSP 1 Score: 395 bits (1014), Expect = 2.71e-131
Identity = 195/341 (57.18%), Postives = 248/341 (72.73%), Query Frame = 0

Query: 1   MTTRAKNGIFRPKIYLADYKEVEPSSAKEALKYPHWKAAMEEEYNALLKNGTWTLTSPPP 60
           M TR+K+GI +PK       + EP + K+ALK P W  AM++EY+ALLKN TWTL   PP
Sbjct: 1   MQTRSKSGIHKPKYPSVLLAQSEPKNVKQALKDPKWLEAMKQEYSALLKNNTWTLVQLPP 60

Query: 61  DKKIVGCKWVFRIKRNTDGSVARYKAHLVAKGFHQTAAIDYQETFSPVVKHVTIRVLLTL 120
           D+  +GCKWVFRIK N+DGSV RYKA LVAKGF Q    D+ ETFSP++K VTIR++LTL
Sbjct: 61  DRNAIGCKWVFRIKENSDGSVNRYKARLVAKGFLQQPGFDFNETFSPIIKPVTIRLILTL 120

Query: 121 AITFGWDLRQIDINNAFLHGILHDSVHMEQPPGFIAPGSGTMVCKLHKALYGLKQAPPAW 180
           AI+  WD+ Q+D+NNAFL+G L+++V+M+QPPGF  P S T+VCKL+KALYGLKQAP  W
Sbjct: 121 AISNHWDIHQLDVNNAFLNGSLNETVYMQQPPGFEVPDS-TLVCKLNKALYGLKQAPRQW 180

Query: 181 FERLSSYLVSLGFRHSRADPSLMYQFKDGRCCYVLIYVDDIVITGNSPTAIQHLIKSLHG 240
           FERL   L+  GF+ S+ DPSL          Y+L+YVDDI+ITGNS    Q LI  L+ 
Sbjct: 181 FERLQYTLLQFGFKASKCDPSLFTYHMQSNIVYLLVYVDDIIITGNSFQFTQQLIDQLNS 240

Query: 241 KFSLKDLGKLGYFLSIEVSYPSKGEIYLSQAKYITNLLQKANMLESNSIATPMISGSITS 300
            FSLK LGKL YFL IEV +   G + L+Q+KYI +LLQ+ NMLE+N+I++PM+S    S
Sbjct: 241 IFSLKQLGKLDYFLGIEVRHLQNGSVLLTQSKYIRDLLQRTNMLEANAISSPMVSSCKLS 300

Query: 301 AFQGESLPDPFQYRSVVGALQYVTLTRPKLAYCVNKVCQFM 341
               ++L D   YRSVVGALQY T+TRP+L++ VNKVCQFM
Sbjct: 301 KHGSDTLADSTFYRSVVGALQYATITRPELSFAVNKVCQFM 340

BLAST of MC09g_new0210 vs. ExPASy TrEMBL
Match: A0A151S6G1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cajanus cajan OX=3821 GN=KK1_027775 PE=4 SV=1)

HSP 1 Score: 390 bits (1001), Expect = 2.41e-129
Identity = 192/344 (55.81%), Postives = 248/344 (72.09%), Query Frame = 0

Query: 1   MTTRAKNGIFRPKIY-LADYKEVEPSSAKEALKYPHWKAAMEEEYNALLKNGTWTLTSPP 60
           MTTRAK GI +P++        +EP + K+AL    W AAM++EYNALL NGTWTL S P
Sbjct: 1   MTTRAKAGIVKPRLQPTLLLTHIEPKNTKQALASDTWLAAMKQEYNALLNNGTWTLVSLP 60

Query: 61  PDKKIVGCKWVFRIKRNTDGSVARYKAHLVAKGFHQTAAIDYQETFSPVVKHVTIRVLLT 120
           P+K  +GCKWVFRIK N+DG+V +YKA LVAKGFHQ    DY ETFSPVVK VTIR++LT
Sbjct: 61  PNKTAIGCKWVFRIKENSDGTVNKYKARLVAKGFHQQFGSDYNETFSPVVKPVTIRLILT 120

Query: 121 LAITFGWDLRQIDINNAFLHGILHDSVHMEQPPGFIAPGSGTMVCKLHKALYGLKQAPPA 180
           LA+T  W ++Q+D+NNAFL+G L + V+M QPPGF +    T+VCKLHKA+YGLKQAP A
Sbjct: 121 LALTHHWSIQQLDVNNAFLNGNLQEEVYMSQPPGFESSDK-TLVCKLHKAIYGLKQAPRA 180

Query: 181 WFERLSSYLVSLGFRHSRADPSLMYQFKDGRCCYVLIYVDDIVITGNSPTAIQHLIKSLH 240
           WF++L + L  L F+ S+ DPSL     D    Y+L+YVDDI+ITGN+ + +Q++I  L+
Sbjct: 181 WFDKLKTTLFQLNFQISKCDPSLFVYSHDNNVIYILVYVDDIIITGNNSSTLQNIIAQLN 240

Query: 241 GKFSLKDLGKLGYFLSIEVSYPSKGEIYLSQAKYITNLLQKANMLESNSIATPMISGSIT 300
             FSLKDLG+L +FL IEV   + G + L+Q+KYI +LL + NM  SN I++PM+SG   
Sbjct: 241 SAFSLKDLGRLDFFLGIEVKSNTDGSLTLTQSKYIRDLLNRTNMENSNPISSPMVSGCKL 300

Query: 301 SAFQGESLPDPFQYRSVVGALQYVTLTRPKLAYCVNKVCQFMHN 343
           S    E   D   YRSVVGALQY T+TRP++++ VNKVCQFM N
Sbjct: 301 SRTGSEKFLDVTLYRSVVGALQYATITRPEISFSVNKVCQFMSN 343

BLAST of MC09g_new0210 vs. ExPASy TrEMBL
Match: A0A5A7UFS3 (Putative mitochondrial protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold504G00520 PE=4 SV=1)

HSP 1 Score: 388 bits (997), Expect = 3.15e-129
Identity = 190/309 (61.49%), Postives = 233/309 (75.40%), Query Frame = 0

Query: 40  MEEEYNALLKNGTWTLTSPPPDKKIVGCKWVFRIKRNTDGSVARYKAHLVAKGFHQTAAI 99
           MEEE+ AL KN TW LT   P++KIVGCKWVF+IKRN+ G +ARYKA LVAKGFHQT  I
Sbjct: 1   MEEEFEALQKNDTWRLTPQNPNQKIVGCKWVFKIKRNSYGPIARYKARLVAKGFHQTPNI 60

Query: 100 DYQETFSPVVKHVTIRVLLTLAITFGWDLRQIDINNAFLHGILHDSVHMEQPPGFIAPGS 159
           DY ETFSPVVK +TI +LLT+AI  GW +RQ+D+NNAFLHG L ++V+MEQ  GF    S
Sbjct: 61  DYNETFSPVVKSITICMLLTIAIMKGWSIRQLDVNNAFLHGNLDENVYMEQIFGFEVKSS 120

Query: 160 GTMVCKLHKALYGLKQAPPAWFERLSSYLVSLGFRHSRADPSLMYQFKDGRCCYVLIYVD 219
             MVC L KALYGLKQAP AW+E LS  L SLGFR S+AD SL+ +     CCY LIYVD
Sbjct: 121 YPMVCHLKKALYGLKQAPRAWYENLSLGLHSLGFRTSKADTSLLIRVTPTSCCYALIYVD 180

Query: 220 DIVITGNSPTAIQHLIKSLHGKFSLKDLGKLGYFLSIEVSYPSKGEIYLSQAKYITNLLQ 279
           D++I G+S   +  L+ SL+ +F+LKDLGKL YFL +EVSYP+ G+++LSQ+KYIT+LLQ
Sbjct: 181 DLIIMGSSKKDVNSLVHSLNSQFALKDLGKLSYFLGVEVSYPTNGDLFLSQSKYITDLLQ 240

Query: 280 KANMLESNSIATPMISGSITSAFQGESLPDPFQYRSVVGALQYVTLTRPKLAYCVNKVCQ 339
           +  ML++  I+TPM+SG + SAFQGE   D    RSVVGALQY TLT P+++Y VNK CQ
Sbjct: 241 RTKMLDAKPISTPMVSGPLLSAFQGEPFHDVHLLRSVVGALQYATLTHPEISYSVNKACQ 300

Query: 340 FMHNLTHIH 348
           FMH   H H
Sbjct: 301 FMHTPKHTH 309

BLAST of MC09g_new0210 vs. ExPASy TrEMBL
Match: A0A392MBU8 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 (Fragment) OS=Trifolium medium OX=97028 GN=A2U01_0005455 PE=4 SV=1)

HSP 1 Score: 387 bits (993), Expect = 9.83e-129
Identity = 185/341 (54.25%), Postives = 250/341 (73.31%), Query Frame = 0

Query: 1   MTTRAKNGIFRPKIYLADYKEVEPSSAKEALKYPHWKAAMEEEYNALLKNGTWTLTSPPP 60
           M TR K G  +PK++LA   E+EP S + AL  P WK AM++EY AL+ N TW+L   PP
Sbjct: 98  MITRGKTGSLKPKVFLA---ELEPKSVRSALSDPKWKQAMQDEYKALMDNKTWSLVPLPP 157

Query: 61  DKKIVGCKWVFRIKRNTDGSVARYKAHLVAKGFHQTAAIDYQETFSPVVKHVTIRVLLTL 120
            +K +GCKW+FR+K N DG+V ++KA LVAKGF QTA  D+ ETFSPV+K +TIR++LTL
Sbjct: 158 HRKAIGCKWIFRVKENPDGTVNKFKARLVAKGFLQTAGFDFTETFSPVIKPITIRIILTL 217

Query: 121 AITFGWDLRQIDINNAFLHGILHDSVHMEQPPGFIAPGSGTMVCKLHKALYGLKQAPPAW 180
           A+TF W ++QID+NNAFL+G+L + V+M QP GF +    ++VCKLHK+LYGLKQAP AW
Sbjct: 218 AVTFKWQVQQIDVNNAFLNGVLQEEVYMAQPSGFESSDK-SLVCKLHKSLYGLKQAPRAW 277

Query: 181 FERLSSYLVSLGFRHSRADPSLMYQFKDGRCCYVLIYVDDIVITGNSPTAIQHLIKSLHG 240
           ++RL+  L+ LGF  S+ DPSL+   ++G C YVLIY+DDI+ITG++P  I  LI  L+ 
Sbjct: 278 YDRLTQALLQLGFVKSKCDPSLLVHNQNGTCTYVLIYMDDILITGSAPHLINDLINKLNM 337

Query: 241 KFSLKDLGKLGYFLSIEVSYPSKGEIYLSQAKYITNLLQKANMLESNSIATPMISGSITS 300
            F+LK LG++ YFL IEV + S G + L+Q+KY+ +LL KA M +S  I +PM+S    S
Sbjct: 338 SFALKRLGQVDYFLGIEVHHLSSGALLLNQSKYVRDLLCKAKMEDSKPIGSPMVSSCRLS 397

Query: 301 AFQGESLPDPFQYRSVVGALQYVTLTRPKLAYCVNKVCQFM 341
            +  +++ DP  YRS+VGALQY TLTRP +A+ VNKVCQFM
Sbjct: 398 KYGTDTMTDPTLYRSIVGALQYATLTRPDIAFSVNKVCQFM 434

BLAST of MC09g_new0210 vs. ExPASy TrEMBL
Match: A0A2K3NI85 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 (Fragment) OS=Trifolium pratense OX=57577 GN=L195_g026060 PE=4 SV=1)

HSP 1 Score: 403 bits (1035), Expect = 1.21e-128
Identity = 199/348 (57.18%), Postives = 256/348 (73.56%), Query Frame = 0

Query: 1   MTTRAKNGIFRPKIYLADYKEVEPSSAKEALKYPHWKAAMEEEYNALLKNGTWTLTSPPP 60
           M TR K G  +PK++LA     EPS+ K+AL  P W  AM+ EY+AL+ N TW+L   P 
Sbjct: 630 MVTRGKTGNLKPKVFLA---HSEPSNVKQALADPQWLTAMKAEYSALMNNQTWSLVPLPA 689

Query: 61  DKKIVGCKWVFRIKRNTDGSVARYKAHLVAKGFHQTAAIDYQETFSPVVKHVTIRVLLTL 120
            ++ +GCKWVFRIK N DGSV +YKA LVAKG+ Q    DY ETFSPVVK VTIR++LTL
Sbjct: 690 HRRAIGCKWVFRIKENPDGSVNKYKARLVAKGYSQEQGFDYNETFSPVVKPVTIRIILTL 749

Query: 121 AITFGWDLRQIDINNAFLHGILHDSVHMEQPPGFIAPGSGTMVCKLHKALYGLKQAPPAW 180
           AI+F WD++QIDINNAFL+GIL + V+M QPPGFI+  S ++VC+LH+ALYGLKQAP AW
Sbjct: 750 AISFKWDIQQIDINNAFLNGILQEEVYMTQPPGFISSDS-SLVCRLHRALYGLKQAPRAW 809

Query: 181 FERLSSYLVSLGFRHSRADPSLMYQFKDGRCCYVLIYVDDIVITGNSPTAIQHLIKSLHG 240
           +ERL+  LV  GF+ S+ DPSL      G   YVL+YVDDI++TG+S + +Q LI  L+ 
Sbjct: 810 YERLTQALVRFGFQPSKCDPSLFVYTHKGITLYVLVYVDDILLTGSSSSLLQDLISKLNS 869

Query: 241 KFSLKDLGKLGYFLSIEVSYPSKGEIYLSQAKYITNLLQKANMLESNSIATPMISGSITS 300
           +F+LK +G+  YFL IEV +   G + L+Q+KYI +LL +ANM E+  I TPM+S    S
Sbjct: 870 QFALKHMGRPDYFLGIEVHHLPNGNMLLTQSKYIRDLLHRANMAEAKGINTPMVSSLKLS 929

Query: 301 AFQGESLPDPFQYRSVVGALQYVTLTRPKLAYCVNKVCQFMHN--LTH 346
            F  +SLPDP +YRS+VGALQYVTLTRP++A+CVNKVCQF+ N  LTH
Sbjct: 930 RFGSDSLPDPHEYRSIVGALQYVTLTRPEIAFCVNKVCQFLSNPLLTH 973

BLAST of MC09g_new0210 vs. TAIR 10
Match: AT4G23160.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 8 )

HSP 1 Score: 249.6 bits (636), Expect = 3.7e-66
Identity = 128/325 (39.38%), Postives = 193/325 (59.38%), Query Frame = 0

Query: 20  KEVEPSSAKEALKYPHWKAAMEEEYNALLKNGTWTLTSPPPDKKIVGCKWVFRIKRNTDG 79
           K  EPS+  EA ++  W  AM++E  A+    TW + + PP+KK +GCKWV++IK N+DG
Sbjct: 82  KAKEPSTYNEAKEFLVWCGAMDDEIGAMETTHTWEICTLPPNKKPIGCKWVYKIKYNSDG 141

Query: 80  SVARYKAHLVAKGFHQTAAIDYQETFSPVVKHVTIRVLLTLAITFGWDLRQIDINNAFLH 139
           ++ RYKA LVAKG+ Q   ID+ ETFSPV K  +++++L ++  + + L Q+DI+NAFL+
Sbjct: 142 TIERYKARLVAKGYTQQEGIDFIETFSPVCKLTSVKLILAISAIYNFTLHQLDISNAFLN 201

Query: 140 GILHDSVHMEQPPGFIAPGSGTM----VCKLHKALYGLKQAPPAWFERLSSYLVSLGFRH 199
           G L + ++M+ PPG+ A    ++    VC L K++YGLKQA   WF + S  L+  GF  
Sbjct: 202 GDLDEEIYMKLPPGYAARQGDSLPPNAVCYLKKSIYGLKQASRQWFLKFSVTLIGFGFVQ 261

Query: 200 SRADPSLMYQFKDGRCCYVLIYVDDIVITGNSPTAIQHLIKSLHGKFSLKDLGKLGYFLS 259
           S +D +   +        VL+YVDDI+I  N+  A+  L   L   F L+DLG L YFL 
Sbjct: 262 SHSDHTYFLKITATLFLCVLVYVDDIIICSNNDAAVDELKSQLKSCFKLRDLGPLKYFLG 321

Query: 260 IEVSYPSKGEIYLSQAKYITNLLQKANMLESNSIATPMISGSITSAFQGESLPDPFQYRS 319
           +E++  + G I + Q KY  +LL +  +L     + PM      SA  G    D   YR 
Sbjct: 322 LEIARSAAG-INICQRKYALDLLDETGLLGCKPSSVPMDPSVTFSAHSGGDFVDAKAYRR 381

Query: 320 VVGALQYVTLTRPKLAYCVNKVCQF 341
           ++G L Y+ +TR  +++ VNK+ QF
Sbjct: 382 LIGRLMYLQITRLDISFAVNKLSQF 405

BLAST of MC09g_new0210 vs. TAIR 10
Match: ATMG00810.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 119.4 bits (298), Expect = 5.8e-27
Identity = 63/136 (46.32%), Postives = 90/136 (66.18%), Query Frame = 0

Query: 213 YVLIYVDDIVITGNSPTAIQHLIKSLHGKFSLKDLGKLGYFLSIEVSYPSKGEIYLSQAK 272
           Y+L+YVDDI++TG+S T +  LI  L   FS+KDLG + YFL I++     G ++LSQ K
Sbjct: 2   YLLLYVDDILLTGSSNTLLNMLIFQLSSTFSMKDLGPVHYFLGIQIKTHPSG-LFLSQTK 61

Query: 273 YITNLLQKANMLESNSIATPM---ISGSITSAFQGESLPDPFQYRSVVGALQYVTLTRPK 332
           Y   +L  A ML+   ++TP+   ++ S+++A      PDP  +RS+VGALQY+TLTRP 
Sbjct: 62  YAEQILNNAGMLDCKPMSTPLPLKLNSSVSTA----KYPDPSDFRSIVGALQYLTLTRPD 121

Query: 333 LAYCVNKVCQFMHNLT 346
           ++Y VN VCQ MH  T
Sbjct: 122 ISYAVNIVCQRMHEPT 132

BLAST of MC09g_new0210 vs. TAIR 10
Match: ATMG00820.1 (Reverse transcriptase (RNA-dependent DNA polymerase) )

HSP 1 Score: 115.2 bits (287), Expect = 1.1e-25
Identity = 62/125 (49.60%), Postives = 80/125 (64.00%), Query Frame = 0

Query: 1   MTTRAKNGI--FRPKIYLADYKEV--EPSSAKEALKYPHWKAAMEEEYNALLKNGTWTLT 60
           M TR+K GI    PK  L     +  EP S   ALK P W  AM+EE +AL +N TW L 
Sbjct: 1   MLTRSKAGINKLNPKYSLTITTTIKKEPKSVIFALKDPGWCQAMQEELDALSRNKTWILV 60

Query: 61  SPPPDKKIVGCKWVFRIKRNTDGSVARYKAHLVAKGFHQTAAIDYQETFSPVVKHVTIRV 120
            PP ++ I+GCKWVF+ K ++DG++ R KA LVAKGFHQ   I + ET+SPVV+  TIR 
Sbjct: 61  PPPVNQNILGCKWVFKTKLHSDGTLDRLKARLVAKGFHQEEGIYFVETYSPVVRTATIRT 120

Query: 121 LLTLA 122
           +L +A
Sbjct: 121 ILNVA 125

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94HW26.1e-9047.59Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O... [more]
Q9ZT942.8e-8745.89Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O... [more]
P109785.0e-6038.81Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
P041464.3e-5137.30Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3[more]
P256003.6e-2629.22Putative transposon Ty5-1 protein YCL074W OS=Saccharomyces cerevisiae (strain AT... [more]
Match NameE-valueIdentityDescription
KYP48058.15.60e-13157.18Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cajanus cajan][more]
KYP50410.14.98e-12955.81Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cajanus cajan][more]
KAA0052371.16.50e-12961.49putative mitochondrial protein [Cucumis melo var. makuwa] >TYK09417.1 putative m... [more]
KAG8489058.11.37e-12853.71hypothetical protein CXB51_017051 [Gossypium anomalum][more]
MCH84623.12.03e-12854.25retrovirus-related Pol polyprotein from transposon TNT 1-94 [Trifolium medium][more]
Match NameE-valueIdentityDescription
A0A151RZS02.71e-13157.18Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cajanus cajan OX=... [more]
A0A151S6G12.41e-12955.81Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cajanus cajan OX=... [more]
A0A5A7UFS33.15e-12961.49Putative mitochondrial protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A392MBU89.83e-12954.25Retrovirus-related Pol polyprotein from transposon TNT 1-94 (Fragment) OS=Trifol... [more]
A0A2K3NI851.21e-12857.18Retrovirus-related Pol polyprotein from transposon TNT 1-94 (Fragment) OS=Trifol... [more]
Match NameE-valueIdentityDescription
AT4G23160.13.7e-6639.38cysteine-rich RLK (RECEPTOR-like protein kinase) 8 [more]
ATMG00810.15.8e-2746.32DNA/RNA polymerases superfamily protein [more]
ATMG00820.11.1e-2549.60Reverse transcriptase (RNA-dependent DNA polymerase) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013103Reverse transcriptase, RNA-dependent DNA polymerasePFAMPF07727RVT_2coord: 50..294
e-value: 3.1E-66
score: 223.4
NoneNo IPR availablePANTHERPTHR45895FAMILY NOT NAMEDcoord: 23..343
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 49..339

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g_new0210.1MC09g_new0210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006259 DNA metabolic process
molecular_function GO:0005488 binding
molecular_function GO:0016779 nucleotidyltransferase activity