MC09g_new0110 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC09g_new0110
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	AB hydrolase-1 domain-containing protein
Location	MC09: 4425608 .. 4429353 (-)
RNA-Seq Expression	MC09g_new0110
Synteny	MC09g_new0110

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CGCATACAAACCCATTACGGAACGGAAGTTTCAATATCTGTCGCTGTTCTCACAACAAAGTCAAATTATTGAAAAATATGCGAAATATGGCCTTTTTCTTTATCATAAAAAATAAAATTAAGAATAAATAAATTTCTTTTCTCGTCTAATTCTTTGAAAATCCCAATTATAAGTCTGTAACAATCGAAGTTCGATTGATTATTTGCCTGGGCCTGAAAGGAAGGGGAAGCGTCTAAGCATCAAATTCATAGCTCTGTTTGCTTCGATAGAATCTCCCGGAAACTCGGAGAAGACCACGACTGAAACCAAACGCCACGAGAACCCATGCGATTTCCCTCGGCTAAGTAGAGGTATAACGAAAGAAGCATTATCAGTTCTTCAATTTCATAGATTTGAGTTCGTTTTCTGCTGTTGGGTTTTCTTTTTAGCTGTGATTAATAGTCGATTAGTCTGTAAAATTGGACCAATACTGCTAAGAGTTGATCCTGAGGAATTCTGTAAAATCTCTCTGTCTACTGACTTTATGTGGAGTGAAGAGGGTTTTCTTATTCTTCATTAATTGAGTATCTATATTGCTTTTGGATTGAGGTTGAAATTTTCATTTGGAAGATGGGGGATATTAGAGCTTGCTTGAGAGTGTTCTTTAAATGCTTTGTATTTTCGTTTTCATAATCGTTTTAAAGAATTATGATTTGGTTGCTTATAAATGTTAGGATTGTTGTTTTCTTGAAATTATGATTCACTAGTTCATGACTTTTTGGGATGCACAACTTTAAACGTTCTGGAAAACAAACTAAAATGGGGACCTTAGAGCTGGTTTGAGAGGGTTTTCAAAATAGTTTTGAGTTTTTGTTTCAAAACAGTTTTACAGAATTTTGATTCGCTCGTTCATGATTTTCATAGGAAGAACAAGATGAAATATCACTTAGTTTTTGTTCTAAAAAACAATTGAAAACGATTCTCCTCTGGCTTGAACATAATTTGTTGTTTAATCAGTTTTCTTTGGCTTATTATTGCAGAACAATTTTCTAGGCATGGAAGTTGTGTCTTATAACTCAGCACTATGCAGGGATCTTGTACTTGTAAGGACTAATTGTGTTAGAAAAAGCTTGAATTCAAGTTGTGTGAACCTCTCTGGCTTGAGGAAGCGCAGAATTGTGTGCGCCAGAATCAAAACCAGAAGCAGATTTTGGGGTTCTTCTATTGTAGAGCAAGTTCTGTTAAAAACTTCCTACCATTGCAAAGGTTTCTTGTCAAAGCATCCCCATGAAAGCTCTCAACCAAAGGTTCTTTGCAGAAGCTACAGTGGCTATGTCATTGATGGAGAAGAAGATTCGATAAGTATATCGGAAAGCAGTGAATCAGCGAGTAAAGTTCAGATTCCCGGTTTGCCCGATGAATCTAATGGTGAATCCAGTGCTCCAATCAGTAGTTGCTTCTGGGAATGGAAACCAAAACTTAGTGTGCACTATGCACAAGCTGGATCTGAAAATGTTAACTCTCCACCAGTACTTTTTCTTCCTGGTTTTGGGGTTGGTTCGTTCCATTATGAGAAGCAACTGAAAGATTTGGGACGTGATTACAGAGTATGGGCCATAGATTTTCTTGGACAGGGCATGTCATTACCAGTTGAAGATCCTACTTCACATTCTAAGCATAGAGATAAATCAGATGGGAAAGATTCTGCTTGGGGTTTTGGAGATGAGACTGAACCATGGGCATCTGAGCTTGTTTACTCCATTGATTTATGGCACGATCAAGTCCGCTATTTCATCGAACAGGTATCCAAAAGAATCATTCATTTTCCTTCATGTTTTTTCCCGTTCCAAGGAATAAAATAGAGAATGATGTACTTGCTTTGAGTAGGAATTTGACGTCTTAAATGGGCCTTGCAGGTCATTGGTGAACCAGTCTACATTGTGGGCAATTCCCTTGGAGGGTTTGTTGCATTGTACTTTGCAGCATTCAATCCTCAATTAGTAAAGGGCGTTACATTGCTAAATGCAACTCCATTCTGGGGATTCCTTCCTAATCCCGTAAGATCTCCAAACTTAGCAAAAATATTTCCTTGGAGTGGAAGGTTTCCGTTGCCAGATAGCGTGAGAAAGCTCACGAAATTCGTGTAAGCTTTTCATTAACCATTCAACGAAGTCTTTATGAATCTTTTCTTTGTTTCCATTCTCTTCTTTACCTTCTGTCCGATTTCAGTTGGCAGAAGATTAGTGACCCCGAAAGTATAGGAGACATACTCAGGCAAGTCTATGCAGATCACACGACAAATGTCGATGACGTATTTTGTCGTATTGTGGAAACAACACAGCATCCAGCTGCAGCTGCATCATTTGCATCAATCATGTTTGCCCCTCAGGGACAACTATCATTCTGGGAAGCTTTATCAAGGTAGGAAATAATTTTGATTCTAAGACTCAATCTCTTGCCCTGTTCTGTTTTTGACTTAAATTTGTTTGTTTCTATTTGTGTAGATGCCATTTGAATTCAGTGCCGGTTTGTCTCATGTATGGGAAAGAAGACCCATGGGTGAGACCAATTTGGGGGCATGAGGTGAAAAAGCGAGTGCCAGAGGCTCCATATTACGAAATCAGCCCTGCAGGCCATTGTCCTCATGATGAAGTTCCTGAGGTATACTTTTGCTGTTCTCTAGATTCATTTCTCAATCAAATACAAAATGGATTCTAAAATCCAAAATCCACATCCTCTGCTGATTTCTTTTACTGATCTACCCTTATCGTTAGTGTCTTTACTTTCCGATATGAAAACCCTAATGAATTAAAATTAGAAACCCTTTGGATATATGAACTCTAATTCACAAAAATAAAACCTTGCCATCCATGTAATTTCAACCCTAAGGATTATGTTAGATTCGAATTGCCTTAGGATTAAATATCTTGAGACGAAAAAAAGTTTATTGGCCTAATAGTCTAACACTTCACAAGATATCATCCTACTCGAATGTTTTTTGGCATGCAATTCAAAGAAACATGAAGATTCAATTAGCTCTAGACTAAACTTGAAAGAACAGCTCGACAGCTAAAGTAATTCGTTAATAATGATATGATCTAAAACAGCCAAATGGCAAGGCCTTAGAAAGCCTCTCAACTAAACCTTATGCAACATCATAAATCATTATGTGAAAACATTAAGGTACCTCTACTTTTAATCCCGAGTCCTTACGAACATCGAATGTTTGATCTAGAAGCTCATGTGATGAATTTGTGGGTTTGGCTGATTTAAGTTCCATTTTCAGTTTCTGGGTGTAAGAAAACTGAAAACGTAGCATGTTATTTAACAGGTGGTGAACTTTTTAGTACGTGGGTGGATCAGAAACTTAGAGACAGAGGGTTCAGTAGAATTGCCTCTTGTGGGGGAACCAGACAACATGGGAGAGGAACACAACAACATTGTTAAGGATTTGGAATATGTAAGAGAAGGATCAAAGAAGTCTGTTTCTGTGCGATTGTATGGATCCAGATCTTCACTTTTTGATCAGATAAGTTCTTTCTTCAGAACTTTACCAATCAAATCTTGACTGACAACTATCCCCCATTGTCCTATGTAATGTGATTGCTTGCTTAGCCTTACGAATCCTCTCTGGTTTGTTGCTTGTTTTACGCTTGCCTTCCACTGCAAAATATTCTCTTCGAATAGGGTTCTCGTTCTTTTTCTTGTTTGTAAAAATGAAAAATGATTTAATCACTATCTCATTTTTTTTTCTCAAGTACAAGCAG

mRNA sequence

ATGGAAGTTGTGTCTTATAACTCAGCACTATGCAGGGATCTTGTACTTGTAAGGACTAATTGTGTTAGAAAAAGCTTGAATTCAAGTTGTGTGAACCTCTCTGGCTTGAGGAAGCGCAGAATTGTGTGCGCCAGAATCAAAACCAGAAGCAGATTTTGGGGTTCTTCTATTGTAGAGCAAGTTCTGTTAAAAACTTCCTACCATTGCAAAGGTTTCTTGTCAAAGCATCCCCATGAAAGCTCTCAACCAAAGGTTCTTTGCAGAAGCTACAGTGGCTATGTCATTGATGGAGAAGAAGATTCGATAAGTATATCGGAAAGCAGTGAATCAGCGAGTAAAGTTCAGATTCCCGGTTTGCCCGATGAATCTAATGGTGAATCCAGTGCTCCAATCAGTAGTTGCTTCTGGGAATGGAAACCAAAACTTAGTGTGCACTATGCACAAGCTGGATCTGAAAATGTTAACTCTCCACCAGTACTTTTTCTTCCTGGTTTTGGGGTTGGTTCGTTCCATTATGAGAAGCAACTGAAAGATTTGGGACGTGATTACAGAGTATGGGCCATAGATTTTCTTGGACAGGGCATGTCATTACCAGTTGAAGATCCTACTTCACATTCTAAGCATAGAGATAAATCAGATGGGAAAGATTCTGCTTGGGGTTTTGGAGATGAGACTGAACCATGGGCATCTGAGCTTGTTTACTCCATTGATTTATGGCACGATCAAGTCCGCTATTTCATCGAACAGGTCATTGGTGAACCAGTCTACATTGTGGGCAATTCCCTTGGAGGGTTTGTTGCATTGTACTTTGCAGCATTCAATCCTCAATTAGTAAAGGGCGTTACATTGCTAAATGCAACTCCATTCTGGGGATTCCTTCCTAATCCCGTAAGATCTCCAAACTTAGCAAAAATATTTCCTTGGAGTGGAAGGTTTCCGTTGCCAGATAGCGTGAGAAAGCTCACGAAATTCGTTTGGCAGAAGATTAGTGACCCCGAAAGTATAGGAGACATACTCAGGCAAGTCTATGCAGATCACACGACAAATGTCGATGACGTATTTTGTCGTATTGTGGAAACAACACAGCATCCAGCTGCAGCTGCATCATTTGCATCAATCATGTTTGCCCCTCAGGGACAACTATCATTCTGGGAAGCTTTATCAAGATGCCATTTGAATTCAGTGCCGGTTTGTCTCATGTATGGGAAAGAAGACCCATGGGTGAGACCAATTTGGGGGCATGAGGTGAAAAAGCGAGTGCCAGAGGCTCCATATTACGAAATCAGCCCTGCAGGCCATTGTCCTCATGATGAAGTTCCTGAGGTGGTGAACTTTTTAGTACGTGGGTGGATCAGAAACTTAGAGACAGAGGGTTCAGTAGAATTGCCTCTTGTGGGGGAACCAGACAACATGGGAGAGGAACACAACAACATTGTTAAGGATTTGGAATATGTAAGAGAAGGATCAAAGAAGTCTGTTTCTGTGCGATTGTATGGATCCAGATCTTCACTTTTTGATCAGATAAGTTCTTTCTTCAGAACTTTACCAATCAAATCTTGA

Coding sequence (CDS)

ATGGAAGTTGTGTCTTATAACTCAGCACTATGCAGGGATCTTGTACTTGTAAGGACTAATTGTGTTAGAAAAAGCTTGAATTCAAGTTGTGTGAACCTCTCTGGCTTGAGGAAGCGCAGAATTGTGTGCGCCAGAATCAAAACCAGAAGCAGATTTTGGGGTTCTTCTATTGTAGAGCAAGTTCTGTTAAAAACTTCCTACCATTGCAAAGGTTTCTTGTCAAAGCATCCCCATGAAAGCTCTCAACCAAAGGTTCTTTGCAGAAGCTACAGTGGCTATGTCATTGATGGAGAAGAAGATTCGATAAGTATATCGGAAAGCAGTGAATCAGCGAGTAAAGTTCAGATTCCCGGTTTGCCCGATGAATCTAATGGTGAATCCAGTGCTCCAATCAGTAGTTGCTTCTGGGAATGGAAACCAAAACTTAGTGTGCACTATGCACAAGCTGGATCTGAAAATGTTAACTCTCCACCAGTACTTTTTCTTCCTGGTTTTGGGGTTGGTTCGTTCCATTATGAGAAGCAACTGAAAGATTTGGGACGTGATTACAGAGTATGGGCCATAGATTTTCTTGGACAGGGCATGTCATTACCAGTTGAAGATCCTACTTCACATTCTAAGCATAGAGATAAATCAGATGGGAAAGATTCTGCTTGGGGTTTTGGAGATGAGACTGAACCATGGGCATCTGAGCTTGTTTACTCCATTGATTTATGGCACGATCAAGTCCGCTATTTCATCGAACAGGTCATTGGTGAACCAGTCTACATTGTGGGCAATTCCCTTGGAGGGTTTGTTGCATTGTACTTTGCAGCATTCAATCCTCAATTAGTAAAGGGCGTTACATTGCTAAATGCAACTCCATTCTGGGGATTCCTTCCTAATCCCGTAAGATCTCCAAACTTAGCAAAAATATTTCCTTGGAGTGGAAGGTTTCCGTTGCCAGATAGCGTGAGAAAGCTCACGAAATTCGTTTGGCAGAAGATTAGTGACCCCGAAAGTATAGGAGACATACTCAGGCAAGTCTATGCAGATCACACGACAAATGTCGATGACGTATTTTGTCGTATTGTGGAAACAACACAGCATCCAGCTGCAGCTGCATCATTTGCATCAATCATGTTTGCCCCTCAGGGACAACTATCATTCTGGGAAGCTTTATCAAGATGCCATTTGAATTCAGTGCCGGTTTGTCTCATGTATGGGAAAGAAGACCCATGGGTGAGACCAATTTGGGGGCATGAGGTGAAAAAGCGAGTGCCAGAGGCTCCATATTACGAAATCAGCCCTGCAGGCCATTGTCCTCATGATGAAGTTCCTGAGGTGGTGAACTTTTTAGTACGTGGGTGGATCAGAAACTTAGAGACAGAGGGTTCAGTAGAATTGCCTCTTGTGGGGGAACCAGACAACATGGGAGAGGAACACAACAACATTGTTAAGGATTTGGAATATGTAAGAGAAGGATCAAAGAAGTCTGTTTCTGTGCGATTGTATGGATCCAGATCTTCACTTTTTGATCAGATAAGTTCTTTCTTCAGAACTTTACCAATCAAATCTTGA

Protein sequence

MEVVSYNSALCRDLVLVRTNCVRKSLNSSCVNLSGLRKRRIVCARIKTRSRFWGSSIVEQVLLKTSYHCKGFLSKHPHESSQPKVLCRSYSGYVIDGEEDSISISESSESASKVQIPGLPDESNGESSAPISSCFWEWKPKLSVHYAQAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLPVEDPTSHSKHRDKSDGKDSAWGFGDETEPWASELVYSIDLWHDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAAFNPQLVKGVTLLNATPFWGFLPNPVRSPNLAKIFPWSGRFPLPDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIVETTQHPAAAASFASIMFAPQGQLSFWEALSRCHLNSVPVCLMYGKEDPWVRPIWGHEVKKRVPEAPYYEISPAGHCPHDEVPEVVNFLVRGWIRNLETEGSVELPLVGEPDNMGEEHNNIVKDLEYVREGSKKSVSVRLYGSRSSLFDQISSFFRTLPIKS

Homology

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BLAST of MC09g_new0110 vs. ExPASy Swiss-Prot
Match: Q9FFZ1 (Pheophytinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PPH PE=1 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 1.6e-171
Identity = 281/423 (66.43%), Postives = 343/423 (81.09%), Query Frame = 0


Query: 92  GYVIDGEEDSISISESSESASKVQIPGLPDESNGESSAPISSCFWEWKPKLSVHYAQAGS 151
           GYV+   +D   I+   ES SKV IPGLPDESNGE +A IS    EWKPKL VHY +AG 
Sbjct: 52  GYVVGENDDLGRIARRGESTSKVLIPGLPDESNGEIAARISHSHCEWKPKLRVHYEKAGC 111

Query: 152 ENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLPVEDPTSHSKHRDK 211
           +N+++P VLFLPGFGVGSFHYEKQL DLGRDYRVWAIDFLGQG+SLP EDPT+ ++    
Sbjct: 112 DNLDAPAVLFLPGFGVGSFHYEKQLTDLGRDYRVWAIDFLGQGLSLPTEDPTTMTEETSS 171

Query: 212 SDGKDSAWGFGDETEPWASELVYSIDLWHDQVRYFIEQVIGEPVYIVGNSLGGFVALYFA 271
           S+ K+  WGFGD+TEPWA +LV+S+DLW DQV+YF+E+VIGEPVYI GNSLGG+VALYFA
Sbjct: 172 SEDKEPFWGFGDKTEPWADQLVFSLDLWRDQVQYFVEEVIGEPVYIAGNSLGGYVALYFA 231

Query: 272 AFNPQLVKGVTLLNATPFWGFLPNPVRSPNLAKIFPWSGRFPLPDSVRKLTKFVWQKISD 331
           A +P LVKGVTLLNATPFWGF PNPVRSP LA++FPW G FPLP+ V+K+T+ VWQKISD
Sbjct: 232 ATHPHLVKGVTLLNATPFWGFFPNPVRSPKLARLFPWPGAFPLPERVKKITELVWQKISD 291

Query: 332 PESIGDILRQVYADHTTNVDDVFCRIVETTQHPAAAASFASIMFAPQGQLSFWEALSRCH 391
           PESI +IL+QVY DH+ NVD VF RIVE TQHPAAAASFASIM AP G+LSF EALSRC 
Sbjct: 292 PESIAEILKQVYTDHSINVDKVFSRIVEVTQHPAAAASFASIMLAPGGELSFSEALSRCK 351

Query: 392 LNSVPVCLMYGKEDPWVRPIWGHEVKKRVPEAPYYEISPAGHCPHDEVPEVVNFLVRGWI 451
            N+V +CLMYG+EDPWVRP+WG ++KK +P APYYEISPAGHCPHDEVPEVVN+L+RGWI
Sbjct: 352 ENNVQICLMYGREDPWVRPLWGKKIKKEIPNAPYYEISPAGHCPHDEVPEVVNYLMRGWI 411

Query: 452 RNLETEGSVELPLVGEPDNMGEEHNNIVKDLEYVREGSKKSVSVRLYGSRSSLFDQISSF 511
           ++LE+ G   LPL+ + +   EE + I +++E+ R+G KK+V++ LYGS  + +  +   
Sbjct: 412 KHLESGGFEALPLLEDTEEDWEE-SRIGREIEFPRDGWKKAVNLWLYGSNYTYWRGVRES 471

Query: 512 FRT 515
           FR+
Sbjct: 472 FRS 473

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BLAST of MC09g_new0110 vs. ExPASy Swiss-Prot
Match: Q8TB40 ((Lyso)-N-acylphosphatidylethanolamine lipase OS=Homo sapiens OX=9606 GN=ABHD4 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 5.6e-07
Identity = 75/313 (23.96%), Postives = 128/313 (40.89%), Query Frame = 0


Query: 152 ENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLPVEDPTSHSKHRDK 211
           E  +  P++ + GFG G   +   +  L     +   D LG G S     P      RD 
Sbjct: 64  EQNDRTPLVMVHGFGGGVGLWILNMDSLSARRTLHTFDLLGFGRSSRPAFP------RDP 123

Query: 212 SDGKDSAWGFGDETEPWASELVYSIDLWHDQVRYFIEQVIGEP-VYIVGNSLGGFVALYF 271
              +D              E V SI+ W         + +G P + ++G+SLGGF+A  +
Sbjct: 124 EGAED--------------EFVTSIETW--------RETMGIPSMILLGHSLGGFLATSY 183

Query: 272 AAFNPQLVKGVTLLNATPFWGFLPNPVRSPNLAKI-FPWSGRFPLPDSVRKLTKFVWQKI 331
           +   P  VK + L++    WGF   P+R  N ++I  P +    +   + +       ++
Sbjct: 184 SIKYPDRVKHLILVDP---WGF---PLRPTNPSEIRAPPAWVKAVASVLGRSNPLAVLRV 243

Query: 332 SDPESIGDILR------QVYADHTTNVDDVFCRIVE--TTQHPAAAASFASIMFAPQGQL 391
           + P   G + R      + +AD     DD     +     Q+P+   +F ++M     + 
Sbjct: 244 AGPWGPGLVQRFRPDFKRKFADFFE--DDTISEYIYHCNAQNPSGETAFKAMM-----ES 303

Query: 392 SFW---EALSRCHL--NSVPVCLMYGKEDPWVRPIWGHEVKKRVPEAPY--YEISPAGHC 448
             W     L R HL    VP+ ++YG  D W+    G +VK + P++     EI  A H 
Sbjct: 304 FGWARRPMLERIHLIRKDVPITMIYG-SDTWIDTSTGKKVKMQRPDSYVRDMEIKGASHH 334

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BLAST of MC09g_new0110 vs. ExPASy Swiss-Prot
Match: O02218 (Abhydrolase domain-containing protein lid-1 OS=Caenorhabditis elegans OX=6239 GN=lid-1 PE=1 SV=2)

HSP 1 Score: 56.2 bits (134), Expect = 1.3e-06
Identity = 39/158 (24.68%), Postives = 67/158 (42.41%), Query Frame = 0


Query: 151 SENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLPVEDPTSHSKHRD 210
           +EN+N   ++F+PG G G   +        +++ V + D LG G S         S+ R 
Sbjct: 66  AENLNGEAIVFIPGLGAGVAMFTANFNSCAKNHAVHSFDPLGFGRS---------SRSR- 125

Query: 211 KSDGKDSAWGFGDETEPWASELVYSIDLWHDQVRYFIEQVIGEPVYIVGNSLGGFVALYF 270
                     F D+      E+V  ++ W   +         E +YI+G++ GG++A  +
Sbjct: 126 ----------FSDDNAIAELEMVEVMEDWRKAMGI-------EKMYIIGHAFGGYLASAY 185

Query: 271 AAFNPQLVKGVTLLNATPFWGFLPNPVRSPNLAKIFPW 309
           A  NP  V  + L++    WGF      +  L K + W
Sbjct: 186 ALENPSRVAHLILVDP---WGFAEKVETTEKLIKPYAW 193

[Close]

BLAST of MC09g_new0110 vs. ExPASy Swiss-Prot
Match: P9WNH2 (Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT2788 PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 6.2e-06
Identity = 62/241 (25.73%), Postives = 104/241 (43.15%), Query Frame = 0


Query: 220 GFGDETEPWASELVYSIDLWHDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAAFNPQLVK 279
           G G   +P A    YS+  + + +R  +  +  E V IVG+SLGG VA+ FA   PQLV 
Sbjct: 73  GHGQSDKPRAD---YSVAAYANGMRDLLSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVD 132

Query: 280 GVTLLNA-------TPFWGFLPNPVRSPNLAKIFPWSGRFPLPDSVRKLTKFVWQKISDP 339
            + L++A          +     P+ S  +A +     R PL     ++   +  K    
Sbjct: 133 RLILVSAGGVTKDVNIVFRLASLPMGSEAMALL-----RLPLVLPAVQIAGRIVGKAIGT 192

Query: 340 ESIGDILRQVYADHTTNVDDVFCRIVETTQHPAAAASFASIMFAP---QGQLSFWEALSR 399
            S+G  L  V             RI++    P A+A+F   + A    +GQ+     L R
Sbjct: 193 TSLGHDLPNV------------LRILDDLPEPTASAAFGRTLRAVVDWRGQMV--TMLDR 252

Query: 400 CHL-NSVPVCLMYGKEDPWVRPIWGHEVKKRVPEAPYYEISPAGHCP-HDEVPEVVNFLV 449
           C+L  ++PV +++G +D  +     H     +P +       +GH P HD+    ++ + 
Sbjct: 253 CYLTEAIPVQIIWGTKDVVLPVRHAHMAHAAMPGSQLEIFEGSGHFPFHDDPARFIDIVE 291

[Close]

BLAST of MC09g_new0110 vs. ExPASy Swiss-Prot
Match: P9WNH3 (Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv2715 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 6.2e-06
Identity = 62/241 (25.73%), Postives = 104/241 (43.15%), Query Frame = 0


Query: 220 GFGDETEPWASELVYSIDLWHDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAAFNPQLVK 279
           G G   +P A    YS+  + + +R  +  +  E V IVG+SLGG VA+ FA   PQLV 
Sbjct: 73  GHGQSDKPRAD---YSVAAYANGMRDLLSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVD 132

Query: 280 GVTLLNA-------TPFWGFLPNPVRSPNLAKIFPWSGRFPLPDSVRKLTKFVWQKISDP 339
            + L++A          +     P+ S  +A +     R PL     ++   +  K    
Sbjct: 133 RLILVSAGGVTKDVNIVFRLASLPMGSEAMALL-----RLPLVLPAVQIAGRIVGKAIGT 192

Query: 340 ESIGDILRQVYADHTTNVDDVFCRIVETTQHPAAAASFASIMFAP---QGQLSFWEALSR 399
            S+G  L  V             RI++    P A+A+F   + A    +GQ+     L R
Sbjct: 193 TSLGHDLPNV------------LRILDDLPEPTASAAFGRTLRAVVDWRGQMV--TMLDR 252

Query: 400 CHL-NSVPVCLMYGKEDPWVRPIWGHEVKKRVPEAPYYEISPAGHCP-HDEVPEVVNFLV 449
           C+L  ++PV +++G +D  +     H     +P +       +GH P HD+    ++ + 
Sbjct: 253 CYLTEAIPVQIIWGTKDVVLPVRHAHMAHAAMPGSQLEIFEGSGHFPFHDDPARFIDIVE 291

[Close]

BLAST of MC09g_new0110 vs. NCBI nr
Match: XP_022149959.1 (pheophytinase, chloroplastic [Momordica charantia] >XP_022149967.1 pheophytinase, chloroplastic [Momordica charantia] >XP_022149976.1 pheophytinase, chloroplastic [Momordica charantia])

HSP 1 Score: 1073 bits (2774), Expect = 0.0
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 0


Query: 1   MEVVSYNSALCRDLVLVRTNCVRKSLNSSCVNLSGLRKRRIVCARIKTRSRFWGSSIVEQ 60
           MEVVSYNSALCRDLVLVRTNCVRKSLNSSCVNLSGLRKRRIVCARIKTRSRFWGSSIVEQ
Sbjct: 1   MEVVSYNSALCRDLVLVRTNCVRKSLNSSCVNLSGLRKRRIVCARIKTRSRFWGSSIVEQ 60

Query: 61  VLLKTSYHCKGFLSKHPHESSQPKVLCRSYSGYVIDGEEDSISISESSESASKVQIPGLP 120
           VLLKTSYHCKGFLSKHPHESSQPKVLCRSYSGYVIDGEEDSISISESSESASKVQIPGLP
Sbjct: 61  VLLKTSYHCKGFLSKHPHESSQPKVLCRSYSGYVIDGEEDSISISESSESASKVQIPGLP 120

Query: 121 DESNGESSAPISSCFWEWKPKLSVHYAQAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLG 180
           DESNGESSAPISSCFWEWKPKLSVHYAQAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLG
Sbjct: 121 DESNGESSAPISSCFWEWKPKLSVHYAQAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLG 180

Query: 181 RDYRVWAIDFLGQGMSLPVEDPTSHSKHRDKSDGKDSAWGFGDETEPWASELVYSIDLWH 240
           RDYRVWAIDFLGQGMSLPVEDPTSHSKHRDKSDGKDSAWGFGDETEPWASELVYSIDLWH
Sbjct: 181 RDYRVWAIDFLGQGMSLPVEDPTSHSKHRDKSDGKDSAWGFGDETEPWASELVYSIDLWH 240

Query: 241 DQVRYFIEQVIGEPVYIVGNSLGGFVALYFAAFNPQLVKGVTLLNATPFWGFLPNPVRSP 300
           DQVRYFIEQVIGEPVYIVGNSLGGFVALYFAAFNPQLVKGVTLLNATPFWGFLPNPVRSP
Sbjct: 241 DQVRYFIEQVIGEPVYIVGNSLGGFVALYFAAFNPQLVKGVTLLNATPFWGFLPNPVRSP 300

Query: 301 NLAKIFPWSGRFPLPDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIVET 360
           NLAKIFPWSGRFPLPDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIVET
Sbjct: 301 NLAKIFPWSGRFPLPDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIVET 360

Query: 361 TQHPAAAASFASIMFAPQGQLSFWEALSRCHLNSVPVCLMYGKEDPWVRPIWGHEVKKRV 420
           TQHPAAAASFASIMFAPQGQLSFWEALSRCHLNSVPVCLMYGKEDPWVRPIWGHEVKKRV
Sbjct: 361 TQHPAAAASFASIMFAPQGQLSFWEALSRCHLNSVPVCLMYGKEDPWVRPIWGHEVKKRV 420

Query: 421 PEAPYYEISPAGHCPHDEVPEVVNFLVRGWIRNLETEGSVELPLVGEPDNMGEEHNNIVK 480
           PEAPYYEISPAGHCPHDEVPEVVNFLVRGWIRNLETEGSVELPLVGEPDNMGEEHNNIVK
Sbjct: 421 PEAPYYEISPAGHCPHDEVPEVVNFLVRGWIRNLETEGSVELPLVGEPDNMGEEHNNIVK 480

Query: 481 DLEYVREGSKKSVSVRLYGSRSSLFDQISSFFRTLPIKS 519
           DLEYVREGSKKSVSVRLYGSRSSLFDQISSFFRTLPIKS
Sbjct: 481 DLEYVREGSKKSVSVRLYGSRSSLFDQISSFFRTLPIKS 519

[Close]

BLAST of MC09g_new0110 vs. NCBI nr
Match: XP_004135952.1 (pheophytinase, chloroplastic [Cucumis sativus] >XP_011659427.1 pheophytinase, chloroplastic [Cucumis sativus] >XP_011659428.1 pheophytinase, chloroplastic [Cucumis sativus] >KGN45086.1 hypothetical protein Csa_015683 [Cucumis sativus])

HSP 1 Score: 852 bits (2201), Expect = 5.35e-309
Identity = 417/521 (80.04%), Postives = 455/521 (87.33%), Query Frame = 0


Query: 1   MEVVSYNSALCRDLVLVRTNCVRKSLNSSCVNLSGLRKRRIVCARIKTRSRF-WGSSIVE 60
           ME +S +S    ++V +RT C RKSLN SCVNLSG R+R+I+CARIK R+RF W      
Sbjct: 1   MEALSCSSIPSSNIVFLRTKCARKSLNPSCVNLSGFRRRKILCARIKARTRFSW------ 60

Query: 61  QVLLKTSYHCKGFLSKHPHESSQPKVLCRSYSGYVIDGEED-SISISESSESASKVQIPG 120
                         SKH HESSQ KVLC SY GYVIDGEE  S+SI ES +SASKVQIPG
Sbjct: 61  --------------SKHHHESSQLKVLCSSYGGYVIDGEEGGSVSIPESGKSASKVQIPG 120

Query: 121 LPDESNGESSAPISSCFWEWKPKLSVHYAQAGSENVNSPPVLFLPGFGVGSFHYEKQLKD 180
           LPDESNGESSA ISS FWEWKPKLSVHY +AGSENV SPPVLFLPGFGVGSFHYEKQLKD
Sbjct: 121 LPDESNGESSAEISSGFWEWKPKLSVHYEKAGSENVKSPPVLFLPGFGVGSFHYEKQLKD 180

Query: 181 LGRDYRVWAIDFLGQGMSLPVEDPTSHSKHRDKSDGKDSAWGFGDETEPWASELVYSIDL 240
           LGRDYRVWAIDFLGQGMSLPVEDPTSHSK  ++SDGKDS+WGFGD+TEPWASELVYSIDL
Sbjct: 181 LGRDYRVWAIDFLGQGMSLPVEDPTSHSKEGNESDGKDSSWGFGDKTEPWASELVYSIDL 240

Query: 241 WHDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAAFNPQLVKGVTLLNATPFWGFLPNPVR 300
           W DQVRYFIEQVIGEPVYIVGNSLGGFVALYFAA NP LVKGVTLLNATPFWGF PNP+R
Sbjct: 241 WQDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAACNPDLVKGVTLLNATPFWGFFPNPIR 300

Query: 301 SPNLAKIFPWSGRFPLPDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIV 360
           SP LAK+FPW G+FPLPD+VRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIV
Sbjct: 301 SPRLAKLFPWGGKFPLPDNVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIV 360

Query: 361 ETTQHPAAAASFASIMFAPQGQLSFWEALSRCHLNSVPVCLMYGKEDPWVRPIWGHEVKK 420
           ETTQHPAAAASFASIMFAPQG+LSFWEALSRCH NSVPVCLMYGKEDPWV+P+WG EVKK
Sbjct: 361 ETTQHPAAAASFASIMFAPQGKLSFWEALSRCHENSVPVCLMYGKEDPWVKPVWGLEVKK 420

Query: 421 RVPEAPYYEISPAGHCPHDEVPEVVNFLVRGWIRNLETEGSVELPLVGEPDNMGEEHNNI 480
           RVPEAPYYEISPAGHCPHDEVPEVVNFL+RGWI+N+ETEG V LPLVGE D +GE+   +
Sbjct: 421 RVPEAPYYEISPAGHCPHDEVPEVVNFLLRGWIKNIETEGLVGLPLVGEQDTIGED--KV 480

Query: 481 VKDLEYVREGSKKSVSVRLYGSRSSLFDQISSFFRTLPIKS 519
           VKDLE++REGSKKSVSVRLYGS SS+ D+I+S F+T+ +K+
Sbjct: 481 VKDLEFLREGSKKSVSVRLYGSPSSVLDRITSLFKTVTVKA 499

[Close]

BLAST of MC09g_new0110 vs. NCBI nr
Match: XP_008461354.1 (PREDICTED: pheophytinase, chloroplastic [Cucumis melo] >XP_008461355.1 PREDICTED: pheophytinase, chloroplastic [Cucumis melo] >KAA0058580.1 pheophytinase [Cucumis melo var. makuwa] >TYK10380.1 pheophytinase [Cucumis melo var. makuwa])

HSP 1 Score: 851 bits (2198), Expect = 1.53e-308
Identity = 418/521 (80.23%), Postives = 455/521 (87.33%), Query Frame = 0


Query: 1   MEVVSYNSALCRDLVLVRTNCVRKSLNSSCVNLSGLRKRRIVCARIKTRSRF-WGSSIVE 60
           MEV+S +S    ++V  RT C RKSLN SCVNLSG R+R+I+CARIK R+RF W      
Sbjct: 1   MEVLSCSSIPSSNIVFPRTKCARKSLNPSCVNLSGFRRRKILCARIKARTRFSW------ 60

Query: 61  QVLLKTSYHCKGFLSKHPHESSQPKVLCRSYSGYVIDGEED-SISISESSESASKVQIPG 120
                         SKH HESSQ KVLCRSY GYV+DGEE  S+SISES ESASKVQIPG
Sbjct: 61  --------------SKHHHESSQLKVLCRSYGGYVLDGEEGGSVSISESGESASKVQIPG 120

Query: 121 LPDESNGESSAPISSCFWEWKPKLSVHYAQAGSENVNSPPVLFLPGFGVGSFHYEKQLKD 180
           LPDESNGESSA ISS FWEWKPKLSVHY +AGSENVNSPPVLFLPGFGVGSFHYEKQLKD
Sbjct: 121 LPDESNGESSAEISSGFWEWKPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKD 180

Query: 181 LGRDYRVWAIDFLGQGMSLPVEDPTSHSKHRDKSDGKDSAWGFGDETEPWASELVYSIDL 240
           LGRDYRVWAIDFLGQGMSLPVEDPTSHS   ++SDGKDS+WGFGD+TEPWASELVYSIDL
Sbjct: 181 LGRDYRVWAIDFLGQGMSLPVEDPTSHSNEGNESDGKDSSWGFGDKTEPWASELVYSIDL 240

Query: 241 WHDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAAFNPQLVKGVTLLNATPFWGFLPNPVR 300
           W DQVRYFIEQVIGEPVYIVGNSLGGFVALYFAA NP LVKGVTLLNATPFWGF PNP R
Sbjct: 241 WQDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAACNPDLVKGVTLLNATPFWGFFPNPTR 300

Query: 301 SPNLAKIFPWSGRFPLPDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIV 360
           SP LAK+FPWSG+FPLPD+VRKLTK VWQKISDPESIGD+LRQVYADHTTNVDDVF RIV
Sbjct: 301 SPRLAKMFPWSGKFPLPDNVRKLTKLVWQKISDPESIGDVLRQVYADHTTNVDDVFSRIV 360

Query: 361 ETTQHPAAAASFASIMFAPQGQLSFWEALSRCHLNSVPVCLMYGKEDPWVRPIWGHEVKK 420
           ETTQHPAAAASFASIMFAPQG+LSFWEAL+RCH NSVPVCLMYGKEDPWV+P+WG EVKK
Sbjct: 361 ETTQHPAAAASFASIMFAPQGKLSFWEALTRCHENSVPVCLMYGKEDPWVKPVWGLEVKK 420

Query: 421 RVPEAPYYEISPAGHCPHDEVPEVVNFLVRGWIRNLETEGSVELPLVGEPDNMGEEHNNI 480
           RVPEAPYYEISPAGHCPHDEVPEVVNFL+RGWIRN+ETEG V LPLVG  D++GE+  N+
Sbjct: 421 RVPEAPYYEISPAGHCPHDEVPEVVNFLLRGWIRNMETEGLVGLPLVGAQDSIGED--NV 480

Query: 481 VKDLEYVREGSKKSVSVRLYGSRSSLFDQISSFFRTLPIKS 519
           VKDLE++REGSKKSVSVRLYGS SS+ D+I+S F+T  +K+
Sbjct: 481 VKDLEFLREGSKKSVSVRLYGSPSSVLDRITSLFKTATVKA 499

[Close]

BLAST of MC09g_new0110 vs. NCBI nr
Match: XP_038898744.1 (pheophytinase, chloroplastic [Benincasa hispida] >XP_038898745.1 pheophytinase, chloroplastic [Benincasa hispida] >XP_038898746.1 pheophytinase, chloroplastic [Benincasa hispida] >XP_038898747.1 pheophytinase, chloroplastic [Benincasa hispida] >XP_038898750.1 pheophytinase, chloroplastic [Benincasa hispida])

HSP 1 Score: 834 bits (2155), Expect = 4.49e-302
Identity = 410/515 (79.61%), Postives = 448/515 (86.99%), Query Frame = 0


Query: 1   MEVVSYNSALCRDLVLVRTNCVRKSLNSSCVNLSGLRKRRIVCARIKTRSRF-WGSSIVE 60
           MEV+S +S    ++V +R  C RK LN SCVNLS LRKR+I+CA+IK R+RF W      
Sbjct: 1   MEVLSCSSVPSSNIVFLRRKCARKILNPSCVNLSSLRKRKILCAKIKARTRFSW------ 60

Query: 61  QVLLKTSYHCKGFLSKHPHESSQPKVLCRSYSGYVIDGEED-SISISESSESASKVQIPG 120
                         SK P ESSQPK+LCRSY GYVIDGEE  S+SI ES ES SKVQIPG
Sbjct: 61  --------------SKQPLESSQPKILCRSYGGYVIDGEEGGSVSIPESGESTSKVQIPG 120

Query: 121 LPDESNGESSAPISSCFWEWKPKLSVHYAQAGSENVNSPPVLFLPGFGVGSFHYEKQLKD 180
           LPDESNGESSA ISS FWEWKPKLSVHY +AG EN+NSPPVLFLPGFGVGSFHY KQLKD
Sbjct: 121 LPDESNGESSAQISSGFWEWKPKLSVHYEKAGCENLNSPPVLFLPGFGVGSFHYAKQLKD 180

Query: 181 LGRDYRVWAIDFLGQGMSLPVEDPTSHSKHRDKSDGKDSAWGFGDETEPWASELVYSIDL 240
           LG DYRVWAIDFLGQGMSLPVEDPTSHSK  ++SDGKDS+WGFG+ETEPWASELVYSIDL
Sbjct: 181 LGCDYRVWAIDFLGQGMSLPVEDPTSHSKEGNESDGKDSSWGFGEETEPWASELVYSIDL 240

Query: 241 WHDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAAFNPQLVKGVTLLNATPFWGFLPNPVR 300
           W DQVRYFIEQVIGEPVYIVGNSLGGFVALYFAA NP+LVKGVTLLNATPFWGF PNP R
Sbjct: 241 WQDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPTR 300

Query: 301 SPNLAKIFPWSGRFPLPDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIV 360
           SP LAK+FPWSG+FPLP+SVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIV
Sbjct: 301 SPRLAKMFPWSGKFPLPESVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIV 360

Query: 361 ETTQHPAAAASFASIMFAPQGQLSFWEALSRCHLNSVPVCLMYGKEDPWVRPIWGHEVKK 420
           ETTQHPAAAASFASIMFAPQG+LSFWEALSRCH+NSVPVCL+YGKEDPWV+P+WG EVKK
Sbjct: 361 ETTQHPAAAASFASIMFAPQGKLSFWEALSRCHMNSVPVCLIYGKEDPWVKPVWGLEVKK 420

Query: 421 RVPEAPYYEISPAGHCPHDEVPEVVNFLVRGWIRNLETEGSVELPLVGEPDNMGEEHNNI 480
           RVPEAPYYEISPAGHCPHDEVPEVVNFL+RGWI+NLETEG VELPLV E + +GE+  N+
Sbjct: 421 RVPEAPYYEISPAGHCPHDEVPEVVNFLLRGWIKNLETEGLVELPLVEESNIIGED--NV 480

Query: 481 VKDLEYVREGSKKSVSVRLYGSRSSLFDQISSFFR 513
           VKDLE++REGSKKSVSV+LYG  SS+ D+ISS  +
Sbjct: 481 VKDLEFLREGSKKSVSVQLYGPPSSVLDRISSLLK 493

[Close]

BLAST of MC09g_new0110 vs. NCBI nr
Match: XP_022953333.1 (pheophytinase, chloroplastic [Cucurbita moschata] >XP_022953335.1 pheophytinase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 834 bits (2154), Expect = 5.69e-302
Identity = 408/521 (78.31%), Postives = 452/521 (86.76%), Query Frame = 0


Query: 1   MEVVSYNSALCRDLVLVRTNCVRKSLNSSCVNLSGLRKRRIVCARIKTRSRFWGSSIVEQ 60
           MEV+S +S    +++ +R+NC  K+LN S +NLSGLRKR I+C RIK R RF        
Sbjct: 1   MEVLSCSSVPNINVLFLRSNCAGKTLNPSFINLSGLRKREILCGRIKARRRF-------- 60

Query: 61  VLLKTSYHCKGFLSKHPHESSQPKVLCRSYSGYVIDGEEDSISISESS-ESASKVQIPGL 120
                               SQPK+LCRSY GYV+DGE  S+SISES  ESA+KVQIPGL
Sbjct: 61  --------------------SQPKILCRSYGGYVVDGEGSSVSISESGGESATKVQIPGL 120

Query: 121 PDESNGESSAPISSCFWEWKPKLSVHYAQAGSENVNSPPVLFLPGFGVGSFHYEKQLKDL 180
           PDESNGESSA ISSCFWEWKPKLSVHY +AG+ENVNSPPVLFLPGFGVGSFHYEKQLKDL
Sbjct: 121 PDESNGESSAQISSCFWEWKPKLSVHYEKAGTENVNSPPVLFLPGFGVGSFHYEKQLKDL 180

Query: 181 GRDYRVWAIDFLGQGMSLPVEDPTSHSKHRDKSDGKDSAWGFGDETEPWASELVYSIDLW 240
           GRDYRVWAIDFLGQGMSLPVEDPTS SK  ++S+G+DS+WGFGDETEPWASELVYSIDLW
Sbjct: 181 GRDYRVWAIDFLGQGMSLPVEDPTSSSKEGNESNGQDSSWGFGDETEPWASELVYSIDLW 240

Query: 241 HDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAAFNPQLVKGVTLLNATPFWGFLPNPVRS 300
            DQVRYFIEQVIGEPVYIVGNSLGGFVALYFAA NP+LVKGVTLLNATPFWGF PNP+RS
Sbjct: 241 QDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRS 300

Query: 301 PNLAKIFPWSGRFPLPDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIVE 360
           P LAKIFPWSG+FPLPDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIVE
Sbjct: 301 PRLAKIFPWSGKFPLPDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIVE 360

Query: 361 TTQHPAAAASFASIMFAPQGQLSFWEALSRCHLNSVPVCLMYGKEDPWVRPIWGHEVKKR 420
           TTQHPAAAASFASIMFAPQG+LSFWEALSRCH NSVPVCL+YGKEDPWV+P+WG +VK+R
Sbjct: 361 TTQHPAAAASFASIMFAPQGKLSFWEALSRCHTNSVPVCLIYGKEDPWVKPVWGLQVKRR 420

Query: 421 VPEAPYYEISPAGHCPHDEVPEVVNFLVRGWIRNLETEGSVELPLVGEPDNMGEEHNNIV 480
           VPEAPYYEISPAGHCPHDEVPEVVNFL+RGWI+NLETEGSV LPLVGEPD++G+   N+V
Sbjct: 421 VPEAPYYEISPAGHCPHDEVPEVVNFLLRGWIKNLETEGSVALPLVGEPDSIGDA--NVV 480

Query: 481 KDLEYVREGSKKSVSVRLYGSRSSLFDQ-ISSFFRTLPIKS 519
           KDLE+ REGSKKSVSVRLYGS SS+ ++ I+S F+T  +K+
Sbjct: 481 KDLEFAREGSKKSVSVRLYGSTSSVVERMITSLFKTARVKA 491

[Close]

BLAST of MC09g_new0110 vs. ExPASy TrEMBL
Match: A0A6J1D8K4 (pheophytinase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018248 PE=4 SV=1)

HSP 1 Score: 1073 bits (2774), Expect = 0.0
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 0


Query: 1   MEVVSYNSALCRDLVLVRTNCVRKSLNSSCVNLSGLRKRRIVCARIKTRSRFWGSSIVEQ 60
           MEVVSYNSALCRDLVLVRTNCVRKSLNSSCVNLSGLRKRRIVCARIKTRSRFWGSSIVEQ
Sbjct: 1   MEVVSYNSALCRDLVLVRTNCVRKSLNSSCVNLSGLRKRRIVCARIKTRSRFWGSSIVEQ 60

Query: 61  VLLKTSYHCKGFLSKHPHESSQPKVLCRSYSGYVIDGEEDSISISESSESASKVQIPGLP 120
           VLLKTSYHCKGFLSKHPHESSQPKVLCRSYSGYVIDGEEDSISISESSESASKVQIPGLP
Sbjct: 61  VLLKTSYHCKGFLSKHPHESSQPKVLCRSYSGYVIDGEEDSISISESSESASKVQIPGLP 120

Query: 121 DESNGESSAPISSCFWEWKPKLSVHYAQAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLG 180
           DESNGESSAPISSCFWEWKPKLSVHYAQAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLG
Sbjct: 121 DESNGESSAPISSCFWEWKPKLSVHYAQAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLG 180

Query: 181 RDYRVWAIDFLGQGMSLPVEDPTSHSKHRDKSDGKDSAWGFGDETEPWASELVYSIDLWH 240
           RDYRVWAIDFLGQGMSLPVEDPTSHSKHRDKSDGKDSAWGFGDETEPWASELVYSIDLWH
Sbjct: 181 RDYRVWAIDFLGQGMSLPVEDPTSHSKHRDKSDGKDSAWGFGDETEPWASELVYSIDLWH 240

Query: 241 DQVRYFIEQVIGEPVYIVGNSLGGFVALYFAAFNPQLVKGVTLLNATPFWGFLPNPVRSP 300
           DQVRYFIEQVIGEPVYIVGNSLGGFVALYFAAFNPQLVKGVTLLNATPFWGFLPNPVRSP
Sbjct: 241 DQVRYFIEQVIGEPVYIVGNSLGGFVALYFAAFNPQLVKGVTLLNATPFWGFLPNPVRSP 300

Query: 301 NLAKIFPWSGRFPLPDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIVET 360
           NLAKIFPWSGRFPLPDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIVET
Sbjct: 301 NLAKIFPWSGRFPLPDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIVET 360

Query: 361 TQHPAAAASFASIMFAPQGQLSFWEALSRCHLNSVPVCLMYGKEDPWVRPIWGHEVKKRV 420
           TQHPAAAASFASIMFAPQGQLSFWEALSRCHLNSVPVCLMYGKEDPWVRPIWGHEVKKRV
Sbjct: 361 TQHPAAAASFASIMFAPQGQLSFWEALSRCHLNSVPVCLMYGKEDPWVRPIWGHEVKKRV 420

Query: 421 PEAPYYEISPAGHCPHDEVPEVVNFLVRGWIRNLETEGSVELPLVGEPDNMGEEHNNIVK 480
           PEAPYYEISPAGHCPHDEVPEVVNFLVRGWIRNLETEGSVELPLVGEPDNMGEEHNNIVK
Sbjct: 421 PEAPYYEISPAGHCPHDEVPEVVNFLVRGWIRNLETEGSVELPLVGEPDNMGEEHNNIVK 480

Query: 481 DLEYVREGSKKSVSVRLYGSRSSLFDQISSFFRTLPIKS 519
           DLEYVREGSKKSVSVRLYGSRSSLFDQISSFFRTLPIKS
Sbjct: 481 DLEYVREGSKKSVSVRLYGSRSSLFDQISSFFRTLPIKS 519

[Close]

BLAST of MC09g_new0110 vs. ExPASy TrEMBL
Match: A0A0A0KBD4 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G420790 PE=4 SV=1)

HSP 1 Score: 852 bits (2201), Expect = 2.59e-309
Identity = 417/521 (80.04%), Postives = 455/521 (87.33%), Query Frame = 0


Query: 1   MEVVSYNSALCRDLVLVRTNCVRKSLNSSCVNLSGLRKRRIVCARIKTRSRF-WGSSIVE 60
           ME +S +S    ++V +RT C RKSLN SCVNLSG R+R+I+CARIK R+RF W      
Sbjct: 1   MEALSCSSIPSSNIVFLRTKCARKSLNPSCVNLSGFRRRKILCARIKARTRFSW------ 60

Query: 61  QVLLKTSYHCKGFLSKHPHESSQPKVLCRSYSGYVIDGEED-SISISESSESASKVQIPG 120
                         SKH HESSQ KVLC SY GYVIDGEE  S+SI ES +SASKVQIPG
Sbjct: 61  --------------SKHHHESSQLKVLCSSYGGYVIDGEEGGSVSIPESGKSASKVQIPG 120

Query: 121 LPDESNGESSAPISSCFWEWKPKLSVHYAQAGSENVNSPPVLFLPGFGVGSFHYEKQLKD 180
           LPDESNGESSA ISS FWEWKPKLSVHY +AGSENV SPPVLFLPGFGVGSFHYEKQLKD
Sbjct: 121 LPDESNGESSAEISSGFWEWKPKLSVHYEKAGSENVKSPPVLFLPGFGVGSFHYEKQLKD 180

Query: 181 LGRDYRVWAIDFLGQGMSLPVEDPTSHSKHRDKSDGKDSAWGFGDETEPWASELVYSIDL 240
           LGRDYRVWAIDFLGQGMSLPVEDPTSHSK  ++SDGKDS+WGFGD+TEPWASELVYSIDL
Sbjct: 181 LGRDYRVWAIDFLGQGMSLPVEDPTSHSKEGNESDGKDSSWGFGDKTEPWASELVYSIDL 240

Query: 241 WHDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAAFNPQLVKGVTLLNATPFWGFLPNPVR 300
           W DQVRYFIEQVIGEPVYIVGNSLGGFVALYFAA NP LVKGVTLLNATPFWGF PNP+R
Sbjct: 241 WQDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAACNPDLVKGVTLLNATPFWGFFPNPIR 300

Query: 301 SPNLAKIFPWSGRFPLPDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIV 360
           SP LAK+FPW G+FPLPD+VRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIV
Sbjct: 301 SPRLAKLFPWGGKFPLPDNVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIV 360

Query: 361 ETTQHPAAAASFASIMFAPQGQLSFWEALSRCHLNSVPVCLMYGKEDPWVRPIWGHEVKK 420
           ETTQHPAAAASFASIMFAPQG+LSFWEALSRCH NSVPVCLMYGKEDPWV+P+WG EVKK
Sbjct: 361 ETTQHPAAAASFASIMFAPQGKLSFWEALSRCHENSVPVCLMYGKEDPWVKPVWGLEVKK 420

Query: 421 RVPEAPYYEISPAGHCPHDEVPEVVNFLVRGWIRNLETEGSVELPLVGEPDNMGEEHNNI 480
           RVPEAPYYEISPAGHCPHDEVPEVVNFL+RGWI+N+ETEG V LPLVGE D +GE+   +
Sbjct: 421 RVPEAPYYEISPAGHCPHDEVPEVVNFLLRGWIKNIETEGLVGLPLVGEQDTIGED--KV 480

Query: 481 VKDLEYVREGSKKSVSVRLYGSRSSLFDQISSFFRTLPIKS 519
           VKDLE++REGSKKSVSVRLYGS SS+ D+I+S F+T+ +K+
Sbjct: 481 VKDLEFLREGSKKSVSVRLYGSPSSVLDRITSLFKTVTVKA 499

[Close]

BLAST of MC09g_new0110 vs. ExPASy TrEMBL
Match: A0A1S3CEH2 (pheophytinase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103499960 PE=4 SV=1)

HSP 1 Score: 851 bits (2198), Expect = 7.42e-309
Identity = 418/521 (80.23%), Postives = 455/521 (87.33%), Query Frame = 0


Query: 1   MEVVSYNSALCRDLVLVRTNCVRKSLNSSCVNLSGLRKRRIVCARIKTRSRF-WGSSIVE 60
           MEV+S +S    ++V  RT C RKSLN SCVNLSG R+R+I+CARIK R+RF W      
Sbjct: 1   MEVLSCSSIPSSNIVFPRTKCARKSLNPSCVNLSGFRRRKILCARIKARTRFSW------ 60

Query: 61  QVLLKTSYHCKGFLSKHPHESSQPKVLCRSYSGYVIDGEED-SISISESSESASKVQIPG 120
                         SKH HESSQ KVLCRSY GYV+DGEE  S+SISES ESASKVQIPG
Sbjct: 61  --------------SKHHHESSQLKVLCRSYGGYVLDGEEGGSVSISESGESASKVQIPG 120

Query: 121 LPDESNGESSAPISSCFWEWKPKLSVHYAQAGSENVNSPPVLFLPGFGVGSFHYEKQLKD 180
           LPDESNGESSA ISS FWEWKPKLSVHY +AGSENVNSPPVLFLPGFGVGSFHYEKQLKD
Sbjct: 121 LPDESNGESSAEISSGFWEWKPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKD 180

Query: 181 LGRDYRVWAIDFLGQGMSLPVEDPTSHSKHRDKSDGKDSAWGFGDETEPWASELVYSIDL 240
           LGRDYRVWAIDFLGQGMSLPVEDPTSHS   ++SDGKDS+WGFGD+TEPWASELVYSIDL
Sbjct: 181 LGRDYRVWAIDFLGQGMSLPVEDPTSHSNEGNESDGKDSSWGFGDKTEPWASELVYSIDL 240

Query: 241 WHDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAAFNPQLVKGVTLLNATPFWGFLPNPVR 300
           W DQVRYFIEQVIGEPVYIVGNSLGGFVALYFAA NP LVKGVTLLNATPFWGF PNP R
Sbjct: 241 WQDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAACNPDLVKGVTLLNATPFWGFFPNPTR 300

Query: 301 SPNLAKIFPWSGRFPLPDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIV 360
           SP LAK+FPWSG+FPLPD+VRKLTK VWQKISDPESIGD+LRQVYADHTTNVDDVF RIV
Sbjct: 301 SPRLAKMFPWSGKFPLPDNVRKLTKLVWQKISDPESIGDVLRQVYADHTTNVDDVFSRIV 360

Query: 361 ETTQHPAAAASFASIMFAPQGQLSFWEALSRCHLNSVPVCLMYGKEDPWVRPIWGHEVKK 420
           ETTQHPAAAASFASIMFAPQG+LSFWEAL+RCH NSVPVCLMYGKEDPWV+P+WG EVKK
Sbjct: 361 ETTQHPAAAASFASIMFAPQGKLSFWEALTRCHENSVPVCLMYGKEDPWVKPVWGLEVKK 420

Query: 421 RVPEAPYYEISPAGHCPHDEVPEVVNFLVRGWIRNLETEGSVELPLVGEPDNMGEEHNNI 480
           RVPEAPYYEISPAGHCPHDEVPEVVNFL+RGWIRN+ETEG V LPLVG  D++GE+  N+
Sbjct: 421 RVPEAPYYEISPAGHCPHDEVPEVVNFLLRGWIRNMETEGLVGLPLVGAQDSIGED--NV 480

Query: 481 VKDLEYVREGSKKSVSVRLYGSRSSLFDQISSFFRTLPIKS 519
           VKDLE++REGSKKSVSVRLYGS SS+ D+I+S F+T  +K+
Sbjct: 481 VKDLEFLREGSKKSVSVRLYGSPSSVLDRITSLFKTATVKA 499

[Close]

BLAST of MC09g_new0110 vs. ExPASy TrEMBL
Match: A0A5D3CG88 (Pheophytinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00220 PE=4 SV=1)

HSP 1 Score: 851 bits (2198), Expect = 7.42e-309
Identity = 418/521 (80.23%), Postives = 455/521 (87.33%), Query Frame = 0


Query: 1   MEVVSYNSALCRDLVLVRTNCVRKSLNSSCVNLSGLRKRRIVCARIKTRSRF-WGSSIVE 60
           MEV+S +S    ++V  RT C RKSLN SCVNLSG R+R+I+CARIK R+RF W      
Sbjct: 1   MEVLSCSSIPSSNIVFPRTKCARKSLNPSCVNLSGFRRRKILCARIKARTRFSW------ 60

Query: 61  QVLLKTSYHCKGFLSKHPHESSQPKVLCRSYSGYVIDGEED-SISISESSESASKVQIPG 120
                         SKH HESSQ KVLCRSY GYV+DGEE  S+SISES ESASKVQIPG
Sbjct: 61  --------------SKHHHESSQLKVLCRSYGGYVLDGEEGGSVSISESGESASKVQIPG 120

Query: 121 LPDESNGESSAPISSCFWEWKPKLSVHYAQAGSENVNSPPVLFLPGFGVGSFHYEKQLKD 180
           LPDESNGESSA ISS FWEWKPKLSVHY +AGSENVNSPPVLFLPGFGVGSFHYEKQLKD
Sbjct: 121 LPDESNGESSAEISSGFWEWKPKLSVHYEKAGSENVNSPPVLFLPGFGVGSFHYEKQLKD 180

Query: 181 LGRDYRVWAIDFLGQGMSLPVEDPTSHSKHRDKSDGKDSAWGFGDETEPWASELVYSIDL 240
           LGRDYRVWAIDFLGQGMSLPVEDPTSHS   ++SDGKDS+WGFGD+TEPWASELVYSIDL
Sbjct: 181 LGRDYRVWAIDFLGQGMSLPVEDPTSHSNEGNESDGKDSSWGFGDKTEPWASELVYSIDL 240

Query: 241 WHDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAAFNPQLVKGVTLLNATPFWGFLPNPVR 300
           W DQVRYFIEQVIGEPVYIVGNSLGGFVALYFAA NP LVKGVTLLNATPFWGF PNP R
Sbjct: 241 WQDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAACNPDLVKGVTLLNATPFWGFFPNPTR 300

Query: 301 SPNLAKIFPWSGRFPLPDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIV 360
           SP LAK+FPWSG+FPLPD+VRKLTK VWQKISDPESIGD+LRQVYADHTTNVDDVF RIV
Sbjct: 301 SPRLAKMFPWSGKFPLPDNVRKLTKLVWQKISDPESIGDVLRQVYADHTTNVDDVFSRIV 360

Query: 361 ETTQHPAAAASFASIMFAPQGQLSFWEALSRCHLNSVPVCLMYGKEDPWVRPIWGHEVKK 420
           ETTQHPAAAASFASIMFAPQG+LSFWEAL+RCH NSVPVCLMYGKEDPWV+P+WG EVKK
Sbjct: 361 ETTQHPAAAASFASIMFAPQGKLSFWEALTRCHENSVPVCLMYGKEDPWVKPVWGLEVKK 420

Query: 421 RVPEAPYYEISPAGHCPHDEVPEVVNFLVRGWIRNLETEGSVELPLVGEPDNMGEEHNNI 480
           RVPEAPYYEISPAGHCPHDEVPEVVNFL+RGWIRN+ETEG V LPLVG  D++GE+  N+
Sbjct: 421 RVPEAPYYEISPAGHCPHDEVPEVVNFLLRGWIRNMETEGLVGLPLVGAQDSIGED--NV 480

Query: 481 VKDLEYVREGSKKSVSVRLYGSRSSLFDQISSFFRTLPIKS 519
           VKDLE++REGSKKSVSVRLYGS SS+ D+I+S F+T  +K+
Sbjct: 481 VKDLEFLREGSKKSVSVRLYGSPSSVLDRITSLFKTATVKA 499

[Close]

BLAST of MC09g_new0110 vs. ExPASy TrEMBL
Match: A0A6J1GPC9 (pheophytinase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111455918 PE=4 SV=1)

HSP 1 Score: 834 bits (2154), Expect = 2.75e-302
Identity = 408/521 (78.31%), Postives = 452/521 (86.76%), Query Frame = 0


Query: 1   MEVVSYNSALCRDLVLVRTNCVRKSLNSSCVNLSGLRKRRIVCARIKTRSRFWGSSIVEQ 60
           MEV+S +S    +++ +R+NC  K+LN S +NLSGLRKR I+C RIK R RF        
Sbjct: 1   MEVLSCSSVPNINVLFLRSNCAGKTLNPSFINLSGLRKREILCGRIKARRRF-------- 60

Query: 61  VLLKTSYHCKGFLSKHPHESSQPKVLCRSYSGYVIDGEEDSISISESS-ESASKVQIPGL 120
                               SQPK+LCRSY GYV+DGE  S+SISES  ESA+KVQIPGL
Sbjct: 61  --------------------SQPKILCRSYGGYVVDGEGSSVSISESGGESATKVQIPGL 120

Query: 121 PDESNGESSAPISSCFWEWKPKLSVHYAQAGSENVNSPPVLFLPGFGVGSFHYEKQLKDL 180
           PDESNGESSA ISSCFWEWKPKLSVHY +AG+ENVNSPPVLFLPGFGVGSFHYEKQLKDL
Sbjct: 121 PDESNGESSAQISSCFWEWKPKLSVHYEKAGTENVNSPPVLFLPGFGVGSFHYEKQLKDL 180

Query: 181 GRDYRVWAIDFLGQGMSLPVEDPTSHSKHRDKSDGKDSAWGFGDETEPWASELVYSIDLW 240
           GRDYRVWAIDFLGQGMSLPVEDPTS SK  ++S+G+DS+WGFGDETEPWASELVYSIDLW
Sbjct: 181 GRDYRVWAIDFLGQGMSLPVEDPTSSSKEGNESNGQDSSWGFGDETEPWASELVYSIDLW 240

Query: 241 HDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAAFNPQLVKGVTLLNATPFWGFLPNPVRS 300
            DQVRYFIEQVIGEPVYIVGNSLGGFVALYFAA NP+LVKGVTLLNATPFWGF PNP+RS
Sbjct: 241 QDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAACNPELVKGVTLLNATPFWGFFPNPIRS 300

Query: 301 PNLAKIFPWSGRFPLPDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIVE 360
           P LAKIFPWSG+FPLPDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIVE
Sbjct: 301 PRLAKIFPWSGKFPLPDSVRKLTKFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIVE 360

Query: 361 TTQHPAAAASFASIMFAPQGQLSFWEALSRCHLNSVPVCLMYGKEDPWVRPIWGHEVKKR 420
           TTQHPAAAASFASIMFAPQG+LSFWEALSRCH NSVPVCL+YGKEDPWV+P+WG +VK+R
Sbjct: 361 TTQHPAAAASFASIMFAPQGKLSFWEALSRCHTNSVPVCLIYGKEDPWVKPVWGLQVKRR 420

Query: 421 VPEAPYYEISPAGHCPHDEVPEVVNFLVRGWIRNLETEGSVELPLVGEPDNMGEEHNNIV 480
           VPEAPYYEISPAGHCPHDEVPEVVNFL+RGWI+NLETEGSV LPLVGEPD++G+   N+V
Sbjct: 421 VPEAPYYEISPAGHCPHDEVPEVVNFLLRGWIKNLETEGSVALPLVGEPDSIGDA--NVV 480

Query: 481 KDLEYVREGSKKSVSVRLYGSRSSLFDQ-ISSFFRTLPIKS 519
           KDLE+ REGSKKSVSVRLYGS SS+ ++ I+S F+T  +K+
Sbjct: 481 KDLEFAREGSKKSVSVRLYGSTSSVVERMITSLFKTARVKA 491

[Close]

BLAST of MC09g_new0110 vs. TAIR 10
Match: AT5G13800.2 (pheophytinase )

HSP 1 Score: 604.0 bits (1556), Expect = 1.1e-172
Identity = 281/423 (66.43%), Postives = 343/423 (81.09%), Query Frame = 0


Query: 92  GYVIDGEEDSISISESSESASKVQIPGLPDESNGESSAPISSCFWEWKPKLSVHYAQAGS 151
           GYV+   +D   I+   ES SKV IPGLPDESNGE +A IS    EWKPKL VHY +AG 
Sbjct: 52  GYVVGENDDLGRIARRGESTSKVLIPGLPDESNGEIAARISHSHCEWKPKLRVHYEKAGC 111

Query: 152 ENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLPVEDPTSHSKHRDK 211
           +N+++P VLFLPGFGVGSFHYEKQL DLGRDYRVWAIDFLGQG+SLP EDPT+ ++    
Sbjct: 112 DNLDAPAVLFLPGFGVGSFHYEKQLTDLGRDYRVWAIDFLGQGLSLPTEDPTTMTEETSS 171

Query: 212 SDGKDSAWGFGDETEPWASELVYSIDLWHDQVRYFIEQVIGEPVYIVGNSLGGFVALYFA 271
           S+ K+  WGFGD+TEPWA +LV+S+DLW DQV+YF+E+VIGEPVYI GNSLGG+VALYFA
Sbjct: 172 SEDKEPFWGFGDKTEPWADQLVFSLDLWRDQVQYFVEEVIGEPVYIAGNSLGGYVALYFA 231

Query: 272 AFNPQLVKGVTLLNATPFWGFLPNPVRSPNLAKIFPWSGRFPLPDSVRKLTKFVWQKISD 331
           A +P LVKGVTLLNATPFWGF PNPVRSP LA++FPW G FPLP+ V+K+T+ VWQKISD
Sbjct: 232 ATHPHLVKGVTLLNATPFWGFFPNPVRSPKLARLFPWPGAFPLPERVKKITELVWQKISD 291

Query: 332 PESIGDILRQVYADHTTNVDDVFCRIVETTQHPAAAASFASIMFAPQGQLSFWEALSRCH 391
           PESI +IL+QVY DH+ NVD VF RIVE TQHPAAAASFASIM AP G+LSF EALSRC 
Sbjct: 292 PESIAEILKQVYTDHSINVDKVFSRIVEVTQHPAAAASFASIMLAPGGELSFSEALSRCK 351

Query: 392 LNSVPVCLMYGKEDPWVRPIWGHEVKKRVPEAPYYEISPAGHCPHDEVPEVVNFLVRGWI 451
            N+V +CLMYG+EDPWVRP+WG ++KK +P APYYEISPAGHCPHDEVPEVVN+L+RGWI
Sbjct: 352 ENNVQICLMYGREDPWVRPLWGKKIKKEIPNAPYYEISPAGHCPHDEVPEVVNYLMRGWI 411

Query: 452 RNLETEGSVELPLVGEPDNMGEEHNNIVKDLEYVREGSKKSVSVRLYGSRSSLFDQISSF 511
           ++LE+ G   LPL+ + +   EE + I +++E+ R+G KK+V++ LYGS  + +  +   
Sbjct: 412 KHLESGGFEALPLLEDTEEDWEE-SRIGREIEFPRDGWKKAVNLWLYGSNYTYWRGVRES 471

Query: 512 FRT 515
           FR+
Sbjct: 472 FRS 473

[Close]

BLAST of MC09g_new0110 vs. TAIR 10
Match: AT5G13800.1 (pheophytinase )

HSP 1 Score: 604.0 bits (1556), Expect = 1.1e-172
Identity = 281/423 (66.43%), Postives = 343/423 (81.09%), Query Frame = 0


Query: 92  GYVIDGEEDSISISESSESASKVQIPGLPDESNGESSAPISSCFWEWKPKLSVHYAQAGS 151
           GYV+   +D   I+   ES SKV IPGLPDESNGE +A IS    EWKPKL VHY +AG 
Sbjct: 52  GYVVGENDDLGRIARRGESTSKVLIPGLPDESNGEIAARISHSHCEWKPKLRVHYEKAGC 111

Query: 152 ENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLPVEDPTSHSKHRDK 211
           +N+++P VLFLPGFGVGSFHYEKQL DLGRDYRVWAIDFLGQG+SLP EDPT+ ++    
Sbjct: 112 DNLDAPAVLFLPGFGVGSFHYEKQLTDLGRDYRVWAIDFLGQGLSLPTEDPTTMTEETSS 171

Query: 212 SDGKDSAWGFGDETEPWASELVYSIDLWHDQVRYFIEQVIGEPVYIVGNSLGGFVALYFA 271
           S+ K+  WGFGD+TEPWA +LV+S+DLW DQV+YF+E+VIGEPVYI GNSLGG+VALYFA
Sbjct: 172 SEDKEPFWGFGDKTEPWADQLVFSLDLWRDQVQYFVEEVIGEPVYIAGNSLGGYVALYFA 231

Query: 272 AFNPQLVKGVTLLNATPFWGFLPNPVRSPNLAKIFPWSGRFPLPDSVRKLTKFVWQKISD 331
           A +P LVKGVTLLNATPFWGF PNPVRSP LA++FPW G FPLP+ V+K+T+ VWQKISD
Sbjct: 232 ATHPHLVKGVTLLNATPFWGFFPNPVRSPKLARLFPWPGAFPLPERVKKITELVWQKISD 291

Query: 332 PESIGDILRQVYADHTTNVDDVFCRIVETTQHPAAAASFASIMFAPQGQLSFWEALSRCH 391
           PESI +IL+QVY DH+ NVD VF RIVE TQHPAAAASFASIM AP G+LSF EALSRC 
Sbjct: 292 PESIAEILKQVYTDHSINVDKVFSRIVEVTQHPAAAASFASIMLAPGGELSFSEALSRCK 351

Query: 392 LNSVPVCLMYGKEDPWVRPIWGHEVKKRVPEAPYYEISPAGHCPHDEVPEVVNFLVRGWI 451
            N+V +CLMYG+EDPWVRP+WG ++KK +P APYYEISPAGHCPHDEVPEVVN+L+RGWI
Sbjct: 352 ENNVQICLMYGREDPWVRPLWGKKIKKEIPNAPYYEISPAGHCPHDEVPEVVNYLMRGWI 411

Query: 452 RNLETEGSVELPLVGEPDNMGEEHNNIVKDLEYVREGSKKSVSVRLYGSRSSLFDQISSF 511
           ++LE+ G   LPL+ + +   EE + I +++E+ R+G KK+V++ LYGS  + +  +   
Sbjct: 412 KHLESGGFEALPLLEDTEEDWEE-SRIGREIEFPRDGWKKAVNLWLYGSNYTYWRGVRES 471

Query: 512 FRT 515
           FR+
Sbjct: 472 FRS 473

[Close]

BLAST of MC09g_new0110 vs. TAIR 10
Match: AT4G36530.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 141.0 bits (354), Expect = 2.8e-33
Identity = 99/324 (30.56%), Postives = 151/324 (46.60%), Query Frame = 0


Query: 135 FWEWKPKLSVHYAQAGSENVNSPPVLFLPGFGVGSFHYEKQLKDLGRDYRVWAIDFLGQG 194
           FWEW+    +HY   G       P++ + GFG   FH+   + +L + Y+V+A+D LG G
Sbjct: 26  FWEWRGH-KIHYVVQG----EGSPLVLIHGFGASVFHWRYNIPELAKKYKVYALDLLGFG 85

Query: 195 MSLPVEDPTSHSKHRDKSDGKDSAWGFGDETEPWASELVYSIDLWHDQVRYFIEQVIGEP 254
            S             DK                  + + Y   +W DQV  F+++V+ EP
Sbjct: 86  WS-------------DK------------------ALIEYDAMVWTDQVIDFMKEVVKEP 145

Query: 255 VYIVGNSLGGFVALYFAAFNPQLVKGVTLLNATPFWGFLPNPVRSPNLAKIFPWSGRFPL 314
             +VGNSLGGF AL  A   P+ V GV LLN+   +          +   I  +  + PL
Sbjct: 146 AVVVGNSLGGFTALSVAVGLPEQVTGVALLNSAGQFAAESRKREEADETVITKFIVK-PL 205

Query: 315 PDSVRKLT-KFVWQKISDPESIGDILRQVYADHTTNVDDVFCRIVETTQHPAAAASFASI 374
            +  +++   F++ +   P  I  +L+ VY D +TNVDD    +VE+   PA   +   +
Sbjct: 206 KEIFQRVVLGFLFWQAKQPSRIESVLKSVYID-STNVDDY---LVESISKPATDPNAGEV 265

Query: 375 MFAPQGQLSFWEALSRCHLNSV------PVCLMYGKEDPWVRPIWGHEVKKRVPEAPYYE 434
            +  +    F    SR  L+SV      P+ L++G  DPWV P    ++K     +    
Sbjct: 266 YY--RLMTRFLTNQSRYTLDSVLSKMTCPLLLVWGDLDPWVGPAKAEKIKAFYSNSSLVH 305

Query: 435 ISPAGHCPHDEVPEVVNFLVRGWI 452
           +  AGHCPHDEVPE VN  +  W+
Sbjct: 326 LQ-AGHCPHDEVPEAVNKALLDWL 305

[Close]

BLAST of MC09g_new0110 vs. TAIR 10
Match: AT4G36530.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 139.4 bits (350), Expect = 8.0e-33
Identity = 118/405 (29.14%), Postives = 179/405 (44.20%), Query Frame = 0


Query: 63  LKTSYHCKGFLSKHPHESSQPKVLCR-------SYSGYVIDG--EEDSISISESSESASK 122
           L  S   + F S     S  P  +CR       S   + I G      +S+ ++S SAS 
Sbjct: 7   LTPSVRTELFSSSSSKRSIFPARICRPRNKCEISRRDFAIRGGIVASGVSVMDTS-SASS 66

Query: 123 VQIPGLPDESNGESSAPISSCFWEWKPKLSVHYAQAGSENVNSPPVLFLPGFGVGSFHYE 182
             + G    S   +  P    FWEW+    +HY   G       P++ + GFG   FH+ 
Sbjct: 67  QSVQG----SERLAFKPEGYNFWEWRGH-KIHYVVQG----EGSPLVLIHGFGASVFHWR 126

Query: 183 KQLKDLGRDYRVWAIDFLGQGMSLPVEDPTSHSKHRDKSDGKDSAWGFGDETEPWASELV 242
             + +L + Y+V+A+D LG G S             DK                  + + 
Sbjct: 127 YNIPELAKKYKVYALDLLGFGWS-------------DK------------------ALIE 186

Query: 243 YSIDLWHDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAAFNPQLVKGVTLLNATPFWGFL 302
           Y   +W DQV  F+++V+ EP  +VGNSLGGF AL  A   P+ V GV LLN+   +   
Sbjct: 187 YDAMVWTDQVIDFMKEVVKEPAVVVGNSLGGFTALSVAVGLPEQVTGVALLNSAGQFAAE 246

Query: 303 PNPVRSPNLAKIFPWSGRFPLPDSVRKLT-KFVWQKISDPESIGDILRQVYADHTTNVDD 362
                  +   I  +  + PL +  +++   F++ +   P  I  +L+ VY D +TNVDD
Sbjct: 247 SRKREEADETVITKFIVK-PLKEIFQRVVLGFLFWQAKQPSRIESVLKSVYID-STNVDD 306

Query: 363 VFCRIVETTQHPAAAASFASIMFAPQGQLSFWEALSRCHLNSV------PVCLMYGKEDP 422
               +VE+   PA   +   + +  +    F    SR  L+SV      P+ L++G  DP
Sbjct: 307 Y---LVESISKPATDPNAGEVYY--RLMTRFLTNQSRYTLDSVLSKMTCPLLLVWGDLDP 362

Query: 423 WVRPIWGHEVKKRVPEAPYYEISPAGHCPHDEVPEVVNFLVRGWI 452
           WV P    ++K     +    +  AGHCPHDEVPE VN  +  W+
Sbjct: 367 WVGPAKAEKIKAFYSNSSLVHLQ-AGHCPHDEVPEAVNKALLDWL 362

[Close]

BLAST of MC09g_new0110 vs. TAIR 10
Match: AT5G19850.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 118.6 bits (296), Expect = 1.5e-26
Identity = 95/353 (26.91%), Postives = 157/353 (44.48%), Query Frame = 0


Query: 105 SESSESASKVQIPGLPDESNGESSAPISSCFWEWKPKLSVHYAQAGSENVNSPPVLFLPG 164
           S +      V    + DE++ E    + +  W+WK   S+ Y  AG+   + P ++ + G
Sbjct: 48  SPTKRGIVSVSCSSVTDEASSE-ELQVRTLTWKWK-GYSIRYQCAGT---SGPALVLVHG 107

Query: 165 FGVGSFHYEKQLKDLGRDYRVWAIDFLGQGMSLPVEDPTSHSKHRDKSDGKDSAWGFGDE 224
           FG  S H+ K    LG+ +RV++ID +G G S             DK + ++    FG E
Sbjct: 108 FGANSDHWRKNTPILGKTHRVYSIDLIGYGYS-------------DKPNPRE----FGGE 167

Query: 225 TEPWASELVYSIDLWHDQVRYFIEQVIGEPVYIVGNSLGGFVALYFAAFNPQLVKGVTLL 284
                    Y+ + W +Q+  F   V+ +  + + NS+GG V L  A   P++ +G+ L+
Sbjct: 168 P-------FYTFETWGEQLNDFCLDVVKDEAFFICNSIGGLVGLQAAVSKPEICRGLMLI 227

Query: 285 NATPFWGFLPNPVRSPNLAKIFPWSGRFPLPDSVRKLT------KFVWQKISDPESIGDI 344
           N +         +R  ++ K  P+ GR P   S + L       K  ++ I+ PE++  I
Sbjct: 228 NIS---------LRMLHIKK-QPFIGR-PFIKSFQNLLRNTPVGKLFFKSIAKPETVKSI 287

Query: 345 LRQVYADHTTNVDDVFCRIVETTQHPAAAASFASIMFAPQGQLSFWEALSRCHLNSVPVC 404
           L Q Y D +   D++   I+     P A   F   +    G L   E L    L   PV 
Sbjct: 288 LCQCYHDSSQVTDELVEAILRPGLEPGAVDVFLEFICYSGGPLP--EDL--LPLVKCPVL 347

Query: 405 LMYGKEDPWVRPIWGHEVKKRVPEAPYYEISPAGHCPHDEVPEVVNFLVRGWI 452
           + +G++DPW     G           +  +  AGHCP DE PE+VN L+  ++
Sbjct: 348 IAWGEKDPWEPIELGRAYSNFDAVEDFVVLPDAGHCPQDEKPEMVNPLIESFV 356

The following BLAST results are available for this feature:

BLAST of MC09g_new0110 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FFZ1	1.6e-171	66.43	Pheophytinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PPH PE=1 SV=1	[more]
Q8TB40	5.6e-07	23.96	(Lyso)-N-acylphosphatidylethanolamine lipase OS=Homo sapiens OX=9606 GN=ABHD4 PE...	[more]
O02218	1.3e-06	24.68	Abhydrolase domain-containing protein lid-1 OS=Caenorhabditis elegans OX=6239 GN...	[more]
P9WNH2	6.2e-06	25.73	Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / ...	[more]
P9WNH3	6.2e-06	25.73	Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 ...	[more]

BLAST of MC09g_new0110 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149959.1	0.0	100.00	pheophytinase, chloroplastic [Momordica charantia] >XP_022149967.1 pheophytinase...	[more]
XP_004135952.1	5.35e-309	80.04	pheophytinase, chloroplastic [Cucumis sativus] >XP_011659427.1 pheophytinase, ch...	[more]
XP_008461354.1	1.53e-308	80.23	PREDICTED: pheophytinase, chloroplastic [Cucumis melo] >XP_008461355.1 PREDICTED...	[more]
XP_038898744.1	4.49e-302	79.61	pheophytinase, chloroplastic [Benincasa hispida] >XP_038898745.1 pheophytinase, ...	[more]
XP_022953333.1	5.69e-302	78.31	pheophytinase, chloroplastic [Cucurbita moschata] >XP_022953335.1 pheophytinase,...	[more]

BLAST of MC09g_new0110 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D8K4	0.0	100.00	pheophytinase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018248 PE=4...	[more]
A0A0A0KBD4	2.59e-309	80.04	AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G420...	[more]
A0A1S3CEH2	7.42e-309	80.23	pheophytinase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103499960 PE=4 SV=1	[more]
A0A5D3CG88	7.42e-309	80.23	Pheophytinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00220 ...	[more]
A0A6J1GPC9	2.75e-302	78.31	pheophytinase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111455918 PE=4 ...	[more]

BLAST of MC09g_new0110 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G13800.2	1.1e-172	66.43	pheophytinase	[more]
AT5G13800.1	1.1e-172	66.43	pheophytinase	[more]
AT4G36530.1	2.8e-33	30.56	alpha/beta-Hydrolases superfamily protein	[more]
AT4G36530.2	8.0e-33	29.14	alpha/beta-Hydrolases superfamily protein	[more]
AT5G19850.1	1.5e-26	26.91	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF12697	Abhydrolase_6	coord: 159..443 e-value: 3.4E-20 score: 73.6
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 110..454 e-value: 1.5E-47 score: 164.5
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 130..453
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 105..126
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 202..220
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 199..222
IPR044211	Pheophytinase, chloroplastic	PANTHER	PTHR47280	PHEOPHYTINASE, CHLOROPLASTIC	coord: 1..514

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC09g_new0110.1	MC09g_new0110.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015996	chlorophyll catabolic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0080124	pheophytinase activity