MC09g1777 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g1777
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionCRS2-associated factor 2, chloroplastic
LocationMC09: 22902893 .. 22906805 (+)
RNA-Seq ExpressionMC09g1777
SyntenyMC09g1777
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGTCTGTAACAATGGCAACTTTACCGTCACTACCAGGACTAAACCTCTTCTCCTCCCTCCCCTCAGCCCCACCGCCGCCGCCGCCGCACGATCCCACATCCACATCCGTCTCTTCTCCTTCCACGCCCATCCCAATTCCCAAATACCCTCCCCCCAAATCCCGCATTCTCCGTTCAAATCCTCCCCCCAATCCCGCTCTCAAAACCTTCCACCGCCGTTCCAAATACTACAAGCCCGTCAAGGACGGAGTCATCTCATCCCACGGCGATCGCGCCGTCGTCATCGGCGAATCGGGCGTCTCCTATCTCCTCCCGGATGCTCCATTCGAGTTCCAATACAGCTACTCCGAGACCCCCAAGGTCAAGCCCATAGCAATTCGCGAGCCGGCGTTTTTGCCCTTTGCACCACCGACGATGCCCAGGCCATGGACCGGAAAAGCCCCACTCAAGAGCTCCAAGAAGAAAATCCCTCTCTTCGATTCCTTCAATCCGCCCCCTCCAGGCACGAAGGGAGTGAAGCAAGTGGAAATGCCTGGTCCATTCCCGCTTGGGAAGTATCCGACGGAGGCAAAGAGCAGAGAGGAGATACTTGGCGAGCCCCTGAAGAATTGGGAGATTCGCATGCTGGTGAAGCCCCATTTGTCGCATAACCGCCAGGTTAATCTTGGTGAGCTATGCATTTCCCCCCCATTTCCATATGAATTTTTACTCACCTTGGTTGTTATATGATCATATATGCTTTGTTGCGATTTCCAGTTCTATGATCGGCTGAAGTTGGTGACCTTTTTTGGTTCGTGTTAGGAAGGGATGGATTGACACATAATATGTTGGAGTTGATACATTCGCATTGGAAGCGGCAGCGTGTTTGTAAAGTTCGTTGTAAAGGTGTTCCCACAGTAGATATGGACAATATCTGTCATCATCTCGAGGTATTTTTTTCGCACAATTATTCATTTTTTAGCCATTTCAATTGAATTGTATGAAGTAATCTATCTTTTTTTTTTCTGGCTTATCTTATGACTATTGTATTTAAATAGGTGCACTACTAGTTTTAGTTTGTGAATTATATAGTCTATATATCACACTGTATTGCCAAGCATGTATTTATGGGTATTGGCCTATCTGTCTTGTGGATTCAGGATTGCATTATAATTCTACGAGAAAGCGAGAAAATATGACATAACTACTCACTCATAACAGATGCTTAAATGTGACAAACTCCAAACCTTAAGACAAGAACTTTTGGTGCTAAATATACCACATTCTAGACCTTGCTATCTCAACTAGTCCATCAAAAGATATTAAAAAAAAAAAAAAAAAATTCCATTATATGCTACTACCAAGATCATTCACATTTGACTGTTAGCTTCTGAGAATTGATCCATCCAGTCCAATATGACAAGGAATTGTCAAGATTCAACAAATCCATTTGCTTTCATGAATGACCAAAAGAATTTTATATCTTGACTCAGAAAAAAAATCATATACTGAAGGTACTTCTTTTAACTGGTTTGCTCCCATTTTGCAATTGTATATATTTTTTCTCCTTTCCCATAAAAATGGTGTCCCAAATATTTTATTTCCTCAACCCTTTTCTAACAATTCTCTTTCTTTTTCTGAGAGATCACTAGTAATTATCTCTGTTCCTTTGCCTGTAAATTTCTTGTGCACTATTCCAGGAAAAAACAGGTGGAAAAATAATTCACCGTGTTGGTGGTGTATTATATCTTTTCCAAGGTAGAAACTACAATTATCGTACTCGCCCCCAATACCCAGTAATGCTCTGGAAACCTGCAGCCCCTGTGTACCCAAAACTTATTCAAGAAGCTCCAGAAGGATTGACAAAAGAGGAAGCTAATAAGCTGAGGATGCAAGGGAAAAATCTTTTGCCAATATGTAAATTAGGTAAATGAGTATATCATTCTCCATAGTCTCCAATTTCTCTTTCTATTATGTTGCTATTGGAGTTCCCTAACAGTATTCGTTAGCTTTTTTCTTCTTCTAGTCGAGCAACTCCATTTGGCAGATATAATCTGTTAACTATTAGTATACCAATAATAGAACTGTTACTAAGTTTATATTATACATCAAATTGAGTAGTTATAAGTAGAGTAAGATGGAAAGAGTGAGGGATTAGGCAAATGCTTGGACAAAAAGTTTTGGGAGAGCGAGCTCCCTTGAGAGGCTATCATTGTTTTTCAATGTCAGGCTCCTATCAACCAAAGATGATCCTTTTATATTATTTTTCTGATTTTCTTAAGGGCCAACTTGGAAAGTGAAAATGTAGTCTTTTTATTTTATTTTTTGTGATAGAGGCAGTGATAGTTTTTGGAGAATATGTAAATTAGGTGAAGAAGCTAATGAGCCAAGAATATAAAAATGGAAAAGGGTACTTTGATATACAAATTTCTTTTTTAATCGGATTGCAATTGTGTACTTGCTTGTTATTCATACATTATGGGCATATTTTAATCGCCTTATAAGTATTGAGCAAAATCTATTTCCCTTTGCAGCAAAAAATGGTGTGTACATTTCCCTGGTGAATGATGTAAGGCATGCCTTTGAAGGAAGCATTTTAGTGAAGATTGATTGCACCGGGATGCATGAAAGTGATTATAAAAAGTTGGGTGCTAAACTCAAGGTTGGCGTACATTTTAATCCTTTAACCTCTGCTTTGTTCCTTTGACCCTTGCAACATTACTTGCATAGAAGTTAGCAGTTAATCCCGAGATAAATCTCTGGATATATGTTGCGGTATCAATGGAACCGAGCTGATTATGCTTTAAACTAAAACTTCAATTACCATGGTTGAGATTTATTTTACACATGATGAAACTACCCACCTTTCTGCCGAGTTAAACCAATAAGTTATGTACATATGTAACTAGCACTTGTGGAAATCATATGATATCTGATCGTTTGATATAAGTCATTGATTATTATTCCTTCATTTTTCTCGACAGGAATTGGTTCCTTGTGTGTTACTGTCATTTGACAACGAAAAGATACTGATGTGGAGGGGAAAAGATTGGAAGTCAGTTATTCCAGAAGATCGTTCTGCTCCACTTCCTCTTCAAGCTAGCACTAATAATTGTTCGGGCTCTTCTGGTAAGTATAGTCCTTTCGTTTAATTACACTTTCTCACTTTTATCAGAAATACAAAGAGGCCATGACATGTAGTCTTAACTCTTAAGTTGTGATGCAAAGTATAAATGACTAATTCTATTTCTCATCAGATTTGAACATAATAACTGCAAATGGCTTTTAAATGTCTTCATCTTTGCTGTTTTTTTTAGGTGAGCCTATCAAGAATAGTAACCGTTCACATGGCAATCATCATACAATCAAAACTAGTCCAAAAATGAAGCTGCTATGGAAGCGTGCTATCGATTCAAACAAGGCATTGTTGCTAAATGTGATTGGTCTTGCTCCTGATGACCTCTTAGAGAAAGTTGAGGAATTTGAGAAAATTTCACAAGCAACTGAGCACTCTTATCCAGCATTTATCTTGTCAAGTGAAGAGAAGGTTAACAGTCCAGATGACAGCGCTGAAAGTCAGGATTACAGCGAACCTAACTATGGCTCTGATGATGATGATGAGGATTGGGAAGCAGACCTCGACCTGTCAGCCCCTTTGGGATCATTACCTGTTGATATAATAGCAAAGAAGCTCAACCCTGAATAGGTACAAGAGACAATTGTTGGACTGGGCTCTTTGGTTGGTATATTTCTTATTCTTGTATAACAAGTTGAAGTGGGAAAAGTTGCAAATTTTCAATGTATCTAGTTGTAAAGAAATTAACTAAACATCGTAACAGCACTTTTAAAAGCATTGAGAATGAAACATATGTTTGGGAAATTGGCTCAAATGACCTCCAAAAACTTTTGAAATGCTCAAATGACCCACTCCTTT

mRNA sequence

AAGTCTGTAACAATGGCAACTTTACCGTCACTACCAGGACTAAACCTCTTCTCCTCCCTCCCCTCAGCCCCACCGCCGCCGCCGCCGCACGATCCCACATCCACATCCGTCTCTTCTCCTTCCACGCCCATCCCAATTCCCAAATACCCTCCCCCCAAATCCCGCATTCTCCGTTCAAATCCTCCCCCCAATCCCGCTCTCAAAACCTTCCACCGCCGTTCCAAATACTACAAGCCCGTCAAGGACGGAGTCATCTCATCCCACGGCGATCGCGCCGTCGTCATCGGCGAATCGGGCGTCTCCTATCTCCTCCCGGATGCTCCATTCGAGTTCCAATACAGCTACTCCGAGACCCCCAAGGTCAAGCCCATAGCAATTCGCGAGCCGGCGTTTTTGCCCTTTGCACCACCGACGATGCCCAGGCCATGGACCGGAAAAGCCCCACTCAAGAGCTCCAAGAAGAAAATCCCTCTCTTCGATTCCTTCAATCCGCCCCCTCCAGGCACGAAGGGAGTGAAGCAAGTGGAAATGCCTGGTCCATTCCCGCTTGGGAAGTATCCGACGGAGGCAAAGAGCAGAGAGGAGATACTTGGCGAGCCCCTGAAGAATTGGGAGATTCGCATGCTGGTGAAGCCCCATTTGTCGCATAACCGCCAGGTTAATCTTGGAAGGGATGGATTGACACATAATATGTTGGAGTTGATACATTCGCATTGGAAGCGGCAGCGTGTTTGTAAAGTTCGTTGTAAAGGTGTTCCCACAGTAGATATGGACAATATCTGTCATCATCTCGAGGAAAAAACAGGTGGAAAAATAATTCACCGTGTTGGTGGTGTATTATATCTTTTCCAAGGTAGAAACTACAATTATCGTACTCGCCCCCAATACCCAGTAATGCTCTGGAAACCTGCAGCCCCTGTGTACCCAAAACTTATTCAAGAAGCTCCAGAAGGATTGACAAAAGAGGAAGCTAATAAGCTGAGGATGCAAGGGAAAAATCTTTTGCCAATATGTAAATTAGCAAAAAATGGTGTGTACATTTCCCTGGTGAATGATGTAAGGCATGCCTTTGAAGGAAGCATTTTAGTGAAGATTGATTGCACCGGGATGCATGAAAGTGATTATAAAAAGTTGGGTGCTAAACTCAAGGAATTGGTTCCTTGTGTGTTACTGTCATTTGACAACGAAAAGATACTGATGTGGAGGGGAAAAGATTGGAAGTCAGTTATTCCAGAAGATCGTTCTGCTCCACTTCCTCTTCAAGCTAGCACTAATAATTGTTCGGGCTCTTCTGGTGAGCCTATCAAGAATAGTAACCGTTCACATGGCAATCATCATACAATCAAAACTAGTCCAAAAATGAAGCTGCTATGGAAGCGTGCTATCGATTCAAACAAGGCATTGTTGCTAAATGTGATTGGTCTTGCTCCTGATGACCTCTTAGAGAAAGTTGAGGAATTTGAGAAAATTTCACAAGCAACTGAGCACTCTTATCCAGCATTTATCTTGTCAAGTGAAGAGAAGGTTAACAGTCCAGATGACAGCGCTGAAAGTCAGGATTACAGCGAACCTAACTATGGCTCTGATGATGATGATGAGGATTGGGAAGCAGACCTCGACCTGTCAGCCCCTTTGGGATCATTACCTGTTGATATAATAGCAAAGAAGCTCAACCCTGAATAGGTACAAGAGACAATTGTTGGACTGGGCTCTTTGGTTGGTATATTTCTTATTCTTGTATAACAAGTTGAAGTGGGAAAAGTTGCAAATTTTCAATGTATCTAGTTGTAAAGAAATTAACTAAACATCGTAACAGCACTTTTAAAAGCATTGAGAATGAAACATATGTTTGGGAAATTGGCTCAAATGACCTCCAAAAACTTTTGAAATGCTCAAATGACCCACTCCTTT

Coding sequence (CDS)

AAGTCTGTAACAATGGCAACTTTACCGTCACTACCAGGACTAAACCTCTTCTCCTCCCTCCCCTCAGCCCCACCGCCGCCGCCGCCGCACGATCCCACATCCACATCCGTCTCTTCTCCTTCCACGCCCATCCCAATTCCCAAATACCCTCCCCCCAAATCCCGCATTCTCCGTTCAAATCCTCCCCCCAATCCCGCTCTCAAAACCTTCCACCGCCGTTCCAAATACTACAAGCCCGTCAAGGACGGAGTCATCTCATCCCACGGCGATCGCGCCGTCGTCATCGGCGAATCGGGCGTCTCCTATCTCCTCCCGGATGCTCCATTCGAGTTCCAATACAGCTACTCCGAGACCCCCAAGGTCAAGCCCATAGCAATTCGCGAGCCGGCGTTTTTGCCCTTTGCACCACCGACGATGCCCAGGCCATGGACCGGAAAAGCCCCACTCAAGAGCTCCAAGAAGAAAATCCCTCTCTTCGATTCCTTCAATCCGCCCCCTCCAGGCACGAAGGGAGTGAAGCAAGTGGAAATGCCTGGTCCATTCCCGCTTGGGAAGTATCCGACGGAGGCAAAGAGCAGAGAGGAGATACTTGGCGAGCCCCTGAAGAATTGGGAGATTCGCATGCTGGTGAAGCCCCATTTGTCGCATAACCGCCAGGTTAATCTTGGAAGGGATGGATTGACACATAATATGTTGGAGTTGATACATTCGCATTGGAAGCGGCAGCGTGTTTGTAAAGTTCGTTGTAAAGGTGTTCCCACAGTAGATATGGACAATATCTGTCATCATCTCGAGGAAAAAACAGGTGGAAAAATAATTCACCGTGTTGGTGGTGTATTATATCTTTTCCAAGGTAGAAACTACAATTATCGTACTCGCCCCCAATACCCAGTAATGCTCTGGAAACCTGCAGCCCCTGTGTACCCAAAACTTATTCAAGAAGCTCCAGAAGGATTGACAAAAGAGGAAGCTAATAAGCTGAGGATGCAAGGGAAAAATCTTTTGCCAATATGTAAATTAGCAAAAAATGGTGTGTACATTTCCCTGGTGAATGATGTAAGGCATGCCTTTGAAGGAAGCATTTTAGTGAAGATTGATTGCACCGGGATGCATGAAAGTGATTATAAAAAGTTGGGTGCTAAACTCAAGGAATTGGTTCCTTGTGTGTTACTGTCATTTGACAACGAAAAGATACTGATGTGGAGGGGAAAAGATTGGAAGTCAGTTATTCCAGAAGATCGTTCTGCTCCACTTCCTCTTCAAGCTAGCACTAATAATTGTTCGGGCTCTTCTGGTGAGCCTATCAAGAATAGTAACCGTTCACATGGCAATCATCATACAATCAAAACTAGTCCAAAAATGAAGCTGCTATGGAAGCGTGCTATCGATTCAAACAAGGCATTGTTGCTAAATGTGATTGGTCTTGCTCCTGATGACCTCTTAGAGAAAGTTGAGGAATTTGAGAAAATTTCACAAGCAACTGAGCACTCTTATCCAGCATTTATCTTGTCAAGTGAAGAGAAGGTTAACAGTCCAGATGACAGCGCTGAAAGTCAGGATTACAGCGAACCTAACTATGGCTCTGATGATGATGATGAGGATTGGGAAGCAGACCTCGACCTGTCAGCCCCTTTGGGATCATTACCTGTTGATATAATAGCAAAGAAGCTCAACCCTGAATAG

Protein sequence

KSVTMATLPSLPGLNLFSSLPSAPPPPPPHDPTSTSVSSPSTPIPIPKYPPPKSRILRSNPPPNPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGESGVSYLLPDAPFEFQYSYSETPKVKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVEMPGPFPLGKYPTEAKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHSHWKRQRVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFQGRNYNYRTRPQYPVMLWKPAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPICKLAKNGVYISLVNDVRHAFEGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEKILMWRGKDWKSVIPEDRSAPLPLQASTNNCSGSSGEPIKNSNRSHGNHHTIKTSPKMKLLWKRAIDSNKALLLNVIGLAPDDLLEKVEEFEKISQATEHSYPAFILSSEEKVNSPDDSAESQDYSEPNYGSDDDDEDWEADLDLSAPLGSLPVDIIAKKLNPE
Homology
BLAST of MC09g1777 vs. ExPASy Swiss-Prot
Match: Q9LDA9 (CRS2-associated factor 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g23400 PE=2 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 1.7e-198
Identity = 361/567 (63.67%), Postives = 432/567 (76.19%), Query Frame = 0

Query: 5   MATLPSLPGLNLFSSLPSAPPPPPPHDPTSTSVSSPSTPIPIPKYPPP-KSRILRSN--- 64
           MA + SL  +NLFSSLPS PP       +S+    P+ PIPIPKY P  ++R  ++N   
Sbjct: 1   MAIVASLRDINLFSSLPSTPPMA----DSSSGTFRPAPPIPIPKYAPSNRNRNQKTNHQT 60

Query: 65  ------PPPNPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGESGVSYLLPDAPFEFQYS 124
                 P  NPALK  H R++YYKPVK+GVISS GDR ++IG+SGVSY LP APFEFQ+S
Sbjct: 61  DTNPKKPQSNPALKLPHHRTRYYKPVKEGVISSDGDRTILIGDSGVSYQLPGAPFEFQFS 120

Query: 125 YSETPKVKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQ 184
           YSETPKVKP+ IREPAF+PFAPPTMPRPWTGKAPLK SKKKIPLFDSFNPPP G  GVK 
Sbjct: 121 YSETPKVKPVGIREPAFMPFAPPTMPRPWTGKAPLKKSKKKIPLFDSFNPPPAGKSGVKY 180

Query: 185 VEMPGPFPLGKYPTEAKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLEL 244
           VEMPGP P G+YP E  +REE+LGEPLK WE  ML+KPH+  NRQVNLGRDG THNMLEL
Sbjct: 181 VEMPGPLPFGRYPKEGMNREEVLGEPLKRWEKGMLIKPHMHDNRQVNLGRDGFTHNMLEL 240

Query: 245 IHSHWKRQRVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFQGRNYNYRTRP 304
           IHSHWKR+RVCKVRCKGVPTVDM+N+C  LEEKTGG+IIHRVGGV+YLF+GRNYNYRTRP
Sbjct: 241 IHSHWKRRRVCKVRCKGVPTVDMNNVCRVLEEKTGGEIIHRVGGVVYLFRGRNYNYRTRP 300

Query: 305 QYPVMLWKPAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPICKLAKNGVYISLVNDVR 364
           QYP+MLWKPAAPVYPKLIQE PEGLTKEEA++ R++GK+L PICKL+KNGVY+SLV DVR
Sbjct: 301 QYPLMLWKPAAPVYPKLIQEVPEGLTKEEAHEFRVKGKSLRPICKLSKNGVYVSLVKDVR 360

Query: 365 HAFEGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEKILMWRGKDWKSVIPEDR 424
            AFE S LVK+DC G+  SDYKK+GAKLKELVPCVLLSFD+E+ILMWRG++WKS   ++ 
Sbjct: 361 DAFELSSLVKVDCPGLEPSDYKKIGAKLKELVPCVLLSFDDEQILMWRGREWKSRFVDNP 420

Query: 425 SAPLPLQASTNNCSGSSGEPIKNSNRSHGNHHTIKTSPKMKLLWKRAIDSNKALLLNVIG 484
             P   + +T N    S +P  +  ++  N  T  +SPKM  LW+RA++S+KA++L  + 
Sbjct: 421 LIPSLSETNTTNELDPSDKP--SEEQTVANPSTTISSPKMISLWQRALESSKAVILEELD 480

Query: 485 LAPDDLLEKVEEFEKISQATEHSYPAFILSSEEKVNSPDDSAESQDYSEPNYGSDDDDED 544
           L PDDLL+KVEE E  S A EH+Y A +LS+ +   + +D  + +D SE  Y   DDD D
Sbjct: 481 LGPDDLLKKVEELEGTSLAAEHTYTAMVLSNTD--GAAEDYVDEKDRSEEYYSDIDDDFD 540

Query: 545 WEA----DLDLSAPLGSLPVDIIAKKL 558
            E      LD   P+GSLPVD I +KL
Sbjct: 541 DECSDDESLDPVGPVGSLPVDKIVRKL 559

BLAST of MC09g1777 vs. ExPASy Swiss-Prot
Match: Q657G7 (CRS2-associated factor 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0323300 PE=3 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 7.1e-149
Identity = 309/607 (50.91%), Postives = 400/607 (65.90%), Query Frame = 0

Query: 9   PSLPG-LNLFSSLPSAPPPPPPHDPTSTSVSSPSTPIPIPKYPPPKSRILRSNPP----- 68
           PS  G  NLFSS P  PP P  +DP     + P  P+P  +  P   R     PP     
Sbjct: 11  PSRAGRANLFSSPP--PPLPNCYDPKHRRPAPP--PLPSARRLPSNRRRRHDQPPNPTTG 70

Query: 69  --PNPALKTFHRRSKYYKPVKDGVISSHGD-------------RAVVIGESGVSYLLPDA 128
              NPA +  H R+ Y KPV     +  G+             RAVV+G SG+S+ LP A
Sbjct: 71  NGGNPAFRAPHLRTAYRKPVPPVAAAGEGEALLAADASDAADGRAVVVGPSGLSFRLPGA 130

Query: 129 PFEFQYSYSETPKVKPIAIREPAFLPFAPPTMPRPWTGKAPL-----KSSKKKIPLFDSF 188
           PF+F++SYSE P+  P+AIREPAFLPFAPPTMPRPWTGKAPL     K+ ++ + L    
Sbjct: 131 PFDFRFSYSECPRAPPVAIREPAFLPFAPPTMPRPWTGKAPLLTKEEKARRRGVRLHTPL 190

Query: 189 NPPPPGTKGVK--QVEMPGPFPLG---KYPTEAKSREEILGEPLKNWEIRMLVKPHLSHN 248
               P T       +E+ G   L      P + +SREE+LGEPL   E+R LVKPH+SHN
Sbjct: 191 GEEAPRTVSAHGIMMEVRGRRKLDLARVSPGDGRSREEVLGEPLTAAEVRDLVKPHISHN 250

Query: 249 RQVNLGRDGLTHNMLELIHSHWKRQRVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVG 308
           RQ+N+GRDGLTHNMLE+IH HW+RQ +CKVRC+GVPTVDM N+C+HLEEK+GGK+IHRVG
Sbjct: 251 RQLNIGRDGLTHNMLEMIHCHWRRQEICKVRCRGVPTVDMKNLCYHLEEKSGGKVIHRVG 310

Query: 309 GVLYLFQGRNYNYRTRPQYPVMLWKPAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPI 368
           GV++L++GRNYN RTRP+YP+MLWKPA PVYPKLIQEAPEGLTKEEA+++R +GK+LLPI
Sbjct: 311 GVVFLYRGRNYNPRTRPRYPLMLWKPATPVYPKLIQEAPEGLTKEEADEMRRRGKDLLPI 370

Query: 369 CKLAKNGVYISLVNDVRHAFEGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEK 428
           CKLAKNG+YI LV DVR AFEGS LVKIDC G++ SDYKK+GAKL++LVPCVLLSFDNE+
Sbjct: 371 CKLAKNGIYIYLVRDVRDAFEGSDLVKIDCEGLNPSDYKKIGAKLRDLVPCVLLSFDNEQ 430

Query: 429 ILMWRGKDWKSVIPEDRSAPLPL--QASTNNCSGSSGEPIKNSNRSHGNHHTIK--TSPK 488
           ILM+RGK+WKS  P+    PL L  +   NN   SS E   +      +   ++    PK
Sbjct: 431 ILMFRGKEWKSRYPK----PLTLIPKIRKNNVPMSSDESSSDEATDDDDRLAVREVLRPK 490

Query: 489 MKLLWKRAIDSNKALLLN---VIGLAPDDLLEKVEEFEKISQATEHSYPAFILSSEEKVN 548
           M  LW  AI+S+ AL+L+   V  L PD LL +VE+F   SQ  EHS+PA +++++E  +
Sbjct: 491 MFELWTNAIESSVALMLDDAEVDALTPDSLLTRVEDFSVTSQVVEHSFPAVLVANDE--S 550

Query: 549 SPD--------DSAES-------QDYSEPNYGSDDD--DEDWEADLDLSAPLGSLPVDII 561
           +PD        D  E+        +++E +  ++DD  ++D    L+ S PLG+LP+D +
Sbjct: 551 NPDVLNAEYTEDEPETGTLEPQQHEFTESSDVAEDDHFEDDMLKRLESSVPLGALPIDAV 607

BLAST of MC09g1777 vs. ExPASy Swiss-Prot
Match: Q84N48 (CRS2-associated factor 2, chloroplastic OS=Zea mays OX=4577 GN=CAF2 PE=1 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 2.4e-144
Identity = 296/589 (50.25%), Postives = 385/589 (65.37%), Query Frame = 0

Query: 19  SLPSAPPPPPPHDPTSTSVSSPSTPIPIPKYPPPKSRILRSNPPPNPALKTFHRRSKYYK 78
           SLP  P  P   DP   S   PS   P    P   +R + +    NPA +  H R+ Y K
Sbjct: 37  SLPLPPVSPRRRDPKKHS-QQPSQEEPTDSGP---TRTVTT----NPAFRAAHLRTAYRK 96

Query: 79  PVKDGVISSHGD-------------RAVVIGESGVSYLLPDAPFEFQYSYSETPKVKPIA 138
           PV     +  G+             RAVV+G SG+S+ LP APF+FQ+SYSE P+  P+A
Sbjct: 97  PVPPAAAAGEGEALLAADPTDAASGRAVVVGPSGLSFRLPGAPFDFQFSYSEAPRAPPLA 156

Query: 139 IREPAFLPFAPPTMPRPWTGKAPL-----KSSKKKIPLFDSFNPPPPGTKG----VKQVE 198
           IREPAFLPFAPPTMPRPWTGKAPL     K+ ++ + L        P T      + +V 
Sbjct: 157 IREPAFLPFAPPTMPRPWTGKAPLLTKEEKARRRGVRLHTPLGQETPQTVSAHGIMMEVR 216

Query: 199 MPGPFPLGKY-PTEAKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELI 258
                 L +  P + +SREE+LGEPL   E+R LVKPH+SHNRQ+N+GRDGLTHNMLE+I
Sbjct: 217 ERRKMDLARVSPGDGRSREEVLGEPLTPSEVRALVKPHISHNRQLNIGRDGLTHNMLEMI 276

Query: 259 HSHWKRQRVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFQGRNYNYRTRPQ 318
           H HW+RQ +CKVRC+GVPTVDM N+C+HLEEK+GGK+IHRVGGV++L++GR+Y+ +TRP+
Sbjct: 277 HCHWRRQEICKVRCRGVPTVDMKNLCYHLEEKSGGKVIHRVGGVVFLYRGRHYDPKTRPR 336

Query: 319 YPVMLWKPAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPICKLAKNGVYISLVNDVRH 378
           YP+MLWKPA PVYPKLI+EAP+G TKEEA+++R +G++LLPICKLAKNG+YI+LV DVR 
Sbjct: 337 YPLMLWKPATPVYPKLIKEAPDGFTKEEADEMRRKGRDLLPICKLAKNGIYITLVKDVRD 396

Query: 379 AFEGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEKILMWRGKDWKSVIPEDRS 438
           AFEGS LVKIDC G++ SDYKK+GAKL++LVPCVLLSFD+E+ILM RGK+WKS      S
Sbjct: 397 AFEGSDLVKIDCEGLNPSDYKKIGAKLRDLVPCVLLSFDDEQILMHRGKEWKS----RYS 456

Query: 439 APLPL--QASTNNCS----GSSGEPIKNSNRSHGNHHTIKTSPKMKLLWKRAIDSNKALL 498
            PL L  +   NN +     +S + + ++N        ++  PKM  LWK A+DS+ ALL
Sbjct: 457 KPLTLIPKVPKNNLAMTSVMNSSDEVSDANTQVAIREVLR--PKMFKLWKSAVDSSLALL 516

Query: 499 LN---VIGLAPDDLLEKVEEFEKISQATEHSYPAFI----------LSSEEKVNSPDDSA 558
           L+      L PD LL  VEEF   SQA EHS+PA +          LS+E   + P+ S 
Sbjct: 517 LDDAEANNLTPDSLLTLVEEFSVTSQAVEHSFPALLVTNGDASTDSLSAEYMNDEPETSV 576

Query: 559 ESQDYSEPNYGSD-DDDEDWEAD----LDLSAPLGSLPVDIIAKKLNPE 561
              +  +     D  DDE ++ D    L+ S PLGSLP+D + ++LN E
Sbjct: 577 AGNEEGQLEQSPDLRDDEHFDVDMFERLESSVPLGSLPIDSMIERLNSE 611

BLAST of MC09g1777 vs. ExPASy Swiss-Prot
Match: Q5VMQ5 (CRS2-associated factor 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0495900 PE=2 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 3.0e-123
Identity = 221/400 (55.25%), Postives = 284/400 (71.00%), Query Frame = 0

Query: 48  KYPPPKSRILRSNPPPNPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGESGVSYLLPDA 107
           K P   ++  R+  P +PA  +  R      P+ +    + G R   + E G++Y L  A
Sbjct: 34  KQPAAVAKRRRAPAPSHPAFSSVIRGRPKKVPIPENGEPAAGVR---VTERGLAYHLDGA 93

Query: 108 PFEFQYSYSETPKVKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPP 167
           PFEFQYSY+ETP+ +P+A+RE  FLPF P   PRPWTG+ PL  S+K++P FDSF  PPP
Sbjct: 94  PFEFQYSYTETPRARPVALREAPFLPFGPEVTPRPWTGRKPLPKSRKELPEFDSFMLPPP 153

Query: 168 GTKGVKQVEMPGPFPLGKYPT-EAKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDG 227
           G KGVK V+ PGPF  G  P  +A SREE+LGEPL   E+  LVK  L   RQ+N+GRDG
Sbjct: 154 GKKGVKPVQSPGPFLAGTEPRYQAASREEVLGEPLTKEEVDELVKATLKTKRQLNIGRDG 213

Query: 228 LTHNMLELIHSHWKRQRVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFQGR 287
           LTHNMLE IHSHWKR+RVCK++CKGV TVDMDN+C  LEEK GGK+IH  GGV++LF+GR
Sbjct: 214 LTHNMLENIHSHWKRKRVCKIKCKGVCTVDMDNVCQQLEEKVGGKVIHHQGGVIFLFRGR 273

Query: 288 NYNYRTRPQYPVMLWKPAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPICKLAKNGVY 347
           NYNYRTRP YP+MLWKPAAPVYP+L+++ P+GLT +EA  +R +G+ L PICKL KNGVY
Sbjct: 274 NYNYRTRPIYPLMLWKPAAPVYPRLVKKIPDGLTPDEAEDMRKRGRQLPPICKLGKNGVY 333

Query: 348 ISLVNDVRHAFEGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEKILMWRGKDW 407
           ++LV  VR AFE   LV++DC+G+++SD +K+GAKLK+LVPC LLSF+ E ILMWRG DW
Sbjct: 334 LNLVKQVREAFEACDLVRVDCSGLNKSDCRKIGAKLKDLVPCTLLSFEFEHILMWRGNDW 393

Query: 408 KSVIP-----EDRSAPLPLQASTNNCSGSSGEPIKNSNRS 442
           KS +P     + + A   +  S    SGS+  PI+  N +
Sbjct: 394 KSSLPPLEENDFKVASDQILNSKEAGSGSALTPIELVNNA 430

BLAST of MC09g1777 vs. ExPASy Swiss-Prot
Match: Q84N49 (CRS2-associated factor 1, chloroplastic OS=Zea mays OX=4577 GN=CAF1 PE=1 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 1.7e-121
Identity = 210/363 (57.85%), Postives = 264/363 (72.73%), Query Frame = 0

Query: 50  PPPKSRILRSNPPPNPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGESGVSYLLPDAPF 109
           PP   +  R     +PA     R      P+ D    + G R   + + G+SY L  APF
Sbjct: 36  PPTGPKRHRRAATSHPAFSAAARGRAKKIPIADTDEPAAGVR---VTDRGISYRLDGAPF 95

Query: 110 EFQYSYSETPKVKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGT 169
           EFQYSY+E P+ +P+A+RE  F+PF P   PRPWTG+ PL  S+K++P FDSF  P PG 
Sbjct: 96  EFQYSYTEAPRARPVALREAPFMPFGPEATPRPWTGRKPLPKSRKELPEFDSFVLPAPGK 155

Query: 170 KGVKQVEMPGPFPLGKYPT-EAKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLT 229
           KGVK V+ PGPF  G  P  ++ SRE+ILGEPL   E+  LVK  L   RQ+N+GRDGLT
Sbjct: 156 KGVKPVQSPGPFLAGMEPRYQSVSREDILGEPLTKEEVSELVKGSLKSKRQLNMGRDGLT 215

Query: 230 HNMLELIHSHWKRQRVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFQGRNY 289
           HNMLE IHSHWKR+RVCK++CKGV T+DMDNICH LEEK GGK+IHR GGV++LF+GRNY
Sbjct: 216 HNMLENIHSHWKRKRVCKIKCKGVCTIDMDNICHQLEEKVGGKVIHRQGGVIFLFRGRNY 275

Query: 290 NYRTRPQYPVMLWKPAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPICKLAKNGVYIS 349
           NYRTRP +P+MLWKP APVYP+L+ + P GLT +EA ++R +G  L PICKL KNGVY +
Sbjct: 276 NYRTRPCFPLMLWKPVAPVYPRLVTKVPGGLTPDEATEMRTRGHQLPPICKLGKNGVYAN 335

Query: 350 LVNDVRHAFEGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEKILMWRGKDWKS 409
           LVN VR AFE   LV++DC+G+++SD +K+GAKLK+LVPC+LLSF+ E ILMWRG DWKS
Sbjct: 336 LVNQVREAFEACDLVRVDCSGLNKSDCRKIGAKLKDLVPCILLSFEFEHILMWRGSDWKS 395

Query: 410 VIP 412
            +P
Sbjct: 396 SLP 395

BLAST of MC09g1777 vs. NCBI nr
Match: XP_038892001.1 (CRS2-associated factor 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 973 bits (2515), Expect = 0.0
Identity = 492/563 (87.39%), Postives = 521/563 (92.54%), Query Frame = 0

Query: 5   MATLPSLPGLNLFSSLPSAPPPPPPHDPTSTSVSSPSTPIPIPKYPPPKSRILRSN---P 64
           MA +PSLPGLNLFSSLPSAPPP   HDP+ TS  SPSTPIPIPKYPPPKSR LR+N    
Sbjct: 1   MARIPSLPGLNLFSSLPSAPPP---HDPSPTS--SPSTPIPIPKYPPPKSRTLRTNNPPK 60

Query: 65  PPNPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGESGVSYLLPDAPFEFQYSYSETPKV 124
           PPNPALKTFHRRS YY+PVKDGVISSHGDR+VVIG+SGVSYLLPDAPFEFQYSYSETPKV
Sbjct: 61  PPNPALKTFHRRSNYYRPVKDGVISSHGDRSVVIGDSGVSYLLPDAPFEFQYSYSETPKV 120

Query: 125 KPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVEMPGPF 184
           KPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQV+MPGPF
Sbjct: 121 KPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVQMPGPF 180

Query: 185 PLGKYPTEAKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHSHWKR 244
           PLG+YP E KSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHSHWKR
Sbjct: 181 PLGQYPKEGKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHSHWKR 240

Query: 245 QRVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFQGRNYNYRTRPQYPVMLW 304
           QRVCKVRCKGVPTVDMDNICHH+EEKTGGKIIHRVGGVLYLF+GRNYNYRTRPQYPVMLW
Sbjct: 241 QRVCKVRCKGVPTVDMDNICHHIEEKTGGKIIHRVGGVLYLFRGRNYNYRTRPQYPVMLW 300

Query: 305 KPAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPICKLAKNGVYISLVNDVRHAFEGSI 364
           KPAAPVYPKLIQEAPEGLTKEEAN+LRM+GKNLLPICKLAKNGVYISLV+DVRHAFEGSI
Sbjct: 301 KPAAPVYPKLIQEAPEGLTKEEANELRMKGKNLLPICKLAKNGVYISLVDDVRHAFEGSI 360

Query: 365 LVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEKILMWRGKDWKSVIPEDRSAPLPLQ 424
           LVK+DC+GMHESDYKKLGAKLKELVPCVLLSFDNE+ILMWRGKDWK VI +DRSAPLP +
Sbjct: 361 LVKVDCSGMHESDYKKLGAKLKELVPCVLLSFDNEQILMWRGKDWKLVISDDRSAPLPTR 420

Query: 425 ASTNNCSGSSGEPIKNSNRSHGNHHTIKTSPKMKLLWKRAIDSNKALLLNVIGLAPDDLL 484
            S+N+ SGSSGE I+N +  HGNHH IKTSPKMKLLW+RAI SNKALLL+ IGLAPDDLL
Sbjct: 421 VSSNDSSGSSGESIENGDLLHGNHHKIKTSPKMKLLWERAIGSNKALLLDEIGLAPDDLL 480

Query: 485 EKVEEFEKISQATEHSYPAFILSSEEKVNSPDDSAESQDYSEPNYGSDDDDEDWEADL-- 544
           EKVEEFEKISQATEHSYPAFI SSE+ V+SPDDS ESQD+S+ NY SDDD  DWE DL  
Sbjct: 481 EKVEEFEKISQATEHSYPAFITSSED-VSSPDDSPESQDHSQANYNSDDD-VDWEGDLFD 540

Query: 545 --DLSAPLGSLPVDIIAKKLNPE 560
             D   PLGSLPVDIIAKKL+PE
Sbjct: 541 NVDPIVPLGSLPVDIIAKKLSPE 556

BLAST of MC09g1777 vs. NCBI nr
Match: XP_008446191.1 (PREDICTED: CRS2-associated factor 2, chloroplastic [Cucumis melo])

HSP 1 Score: 965 bits (2495), Expect = 0.0
Identity = 489/566 (86.40%), Postives = 526/566 (92.93%), Query Frame = 0

Query: 1   KSVTMATLPSLPGLNLFSSLPSAPPPPPPHDPTSTSVSSPSTPIPIPKYPPPKSRILRSN 60
           +SVTMAT+PSLPGL LFSSLPSAPPP   H+P+ TS  SPSTPIPIPKYPPPKSR LR+N
Sbjct: 52  RSVTMATIPSLPGLTLFSSLPSAPPP---HEPSPTS--SPSTPIPIPKYPPPKSRTLRTN 111

Query: 61  PPP---NPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGESGVSYLLPDAPFEFQYSYSE 120
            PP   NPALKTFHRRSKYYKPVKDGVISS+G+RAVVIG+SGVSYLLP APFEFQYSYSE
Sbjct: 112 NPPKSPNPALKTFHRRSKYYKPVKDGVISSNGERAVVIGDSGVSYLLPGAPFEFQYSYSE 171

Query: 121 TPKVKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVEM 180
           TP VKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQV++
Sbjct: 172 TPNVKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVQL 231

Query: 181 PGPFPLGKYPTEAKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHS 240
           PGPFPLG+YP E KSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHS
Sbjct: 232 PGPFPLGQYPKEGKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHS 291

Query: 241 HWKRQRVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFQGRNYNYRTRPQYP 300
           HWKRQRVCKVRCKGVPTVDMDNICHH+EEKTGGKIIHRVGGVLYLF+GRNYNYRTRPQYP
Sbjct: 292 HWKRQRVCKVRCKGVPTVDMDNICHHIEEKTGGKIIHRVGGVLYLFRGRNYNYRTRPQYP 351

Query: 301 VMLWKPAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPICKLAKNGVYISLVNDVRHAF 360
           VMLWKPAAPVYPKLIQEAPEGLTKEEAN+LRM+GKNLLPICKLAKNGVYISLV+DVRHAF
Sbjct: 352 VMLWKPAAPVYPKLIQEAPEGLTKEEANELRMKGKNLLPICKLAKNGVYISLVDDVRHAF 411

Query: 361 EGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEKILMWRGKDWKSVIPEDRSAP 420
           EGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNE+ILMWRGKDWKSVI +DRSAP
Sbjct: 412 EGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEQILMWRGKDWKSVISDDRSAP 471

Query: 421 LPLQASTNNCSGSSGEPIKNSNRSHGNHHTIKTSPKMKLLWKRAIDSNKALLLNVIGLAP 480
           LP +AS+N+  GSSGE ++NS+  +GNHHTIKTSPKMKLLW+RAIDSNKAL+L+ IGLAP
Sbjct: 472 LPSRASSNDSLGSSGESVENSDLLNGNHHTIKTSPKMKLLWERAIDSNKALMLDEIGLAP 531

Query: 481 DDLLEKVEEFEKISQATEHSYPAFILSSEEKVNSPDDSAESQDYSEPNYGSDDD---DED 540
           D+LLE+VEEFE+ISQATEHSYPAFI SSEE V+SP DS ESQD+SE NY SDDD   +ED
Sbjct: 532 DELLERVEEFERISQATEHSYPAFITSSEE-VSSPADSPESQDHSEANYNSDDDVGREED 591

Query: 541 WEADLDLSAPLGSLPVDIIAKKLNPE 560
              ++D   PLGSLPVDIIAKKL+ E
Sbjct: 592 LFDNVDPLVPLGSLPVDIIAKKLSSE 611

BLAST of MC09g1777 vs. NCBI nr
Match: ADN33825.1 (RNA splicing factor [Cucumis melo subsp. melo] >KAA0034302.1 RNA splicing factor [Cucumis melo var. makuwa])

HSP 1 Score: 965 bits (2495), Expect = 0.0
Identity = 489/566 (86.40%), Postives = 526/566 (92.93%), Query Frame = 0

Query: 1   KSVTMATLPSLPGLNLFSSLPSAPPPPPPHDPTSTSVSSPSTPIPIPKYPPPKSRILRSN 60
           +SVTMAT+PSLPGL LFSSLPSAPPP   H+P+ TS  SPSTPIPIPKYPPPKSR LR+N
Sbjct: 44  RSVTMATIPSLPGLTLFSSLPSAPPP---HEPSPTS--SPSTPIPIPKYPPPKSRTLRTN 103

Query: 61  PPP---NPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGESGVSYLLPDAPFEFQYSYSE 120
            PP   NPALKTFHRRSKYYKPVKDGVISS+G+RAVVIG+SGVSYLLP APFEFQYSYSE
Sbjct: 104 NPPKSPNPALKTFHRRSKYYKPVKDGVISSNGERAVVIGDSGVSYLLPGAPFEFQYSYSE 163

Query: 121 TPKVKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVEM 180
           TP VKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQV++
Sbjct: 164 TPNVKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVQL 223

Query: 181 PGPFPLGKYPTEAKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHS 240
           PGPFPLG+YP E KSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHS
Sbjct: 224 PGPFPLGQYPKEGKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHS 283

Query: 241 HWKRQRVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFQGRNYNYRTRPQYP 300
           HWKRQRVCKVRCKGVPTVDMDNICHH+EEKTGGKIIHRVGGVLYLF+GRNYNYRTRPQYP
Sbjct: 284 HWKRQRVCKVRCKGVPTVDMDNICHHIEEKTGGKIIHRVGGVLYLFRGRNYNYRTRPQYP 343

Query: 301 VMLWKPAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPICKLAKNGVYISLVNDVRHAF 360
           VMLWKPAAPVYPKLIQEAPEGLTKEEAN+LRM+GKNLLPICKLAKNGVYISLV+DVRHAF
Sbjct: 344 VMLWKPAAPVYPKLIQEAPEGLTKEEANELRMKGKNLLPICKLAKNGVYISLVDDVRHAF 403

Query: 361 EGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEKILMWRGKDWKSVIPEDRSAP 420
           EGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNE+ILMWRGKDWKSVI +DRSAP
Sbjct: 404 EGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEQILMWRGKDWKSVISDDRSAP 463

Query: 421 LPLQASTNNCSGSSGEPIKNSNRSHGNHHTIKTSPKMKLLWKRAIDSNKALLLNVIGLAP 480
           LP +AS+N+  GSSGE ++NS+  +GNHHTIKTSPKMKLLW+RAIDSNKAL+L+ IGLAP
Sbjct: 464 LPSRASSNDSLGSSGESVENSDLLNGNHHTIKTSPKMKLLWERAIDSNKALMLDEIGLAP 523

Query: 481 DDLLEKVEEFEKISQATEHSYPAFILSSEEKVNSPDDSAESQDYSEPNYGSDDD---DED 540
           D+LLE+VEEFE+ISQATEHSYPAFI SSEE V+SP DS ESQD+SE NY SDDD   +ED
Sbjct: 524 DELLERVEEFERISQATEHSYPAFITSSEE-VSSPADSPESQDHSEANYNSDDDVGREED 583

Query: 541 WEADLDLSAPLGSLPVDIIAKKLNPE 560
              ++D   PLGSLPVDIIAKKL+ E
Sbjct: 584 LFDNVDPLVPLGSLPVDIIAKKLSSE 603

BLAST of MC09g1777 vs. NCBI nr
Match: XP_022956896.1 (CRS2-associated factor 2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 961 bits (2483), Expect = 0.0
Identity = 490/562 (87.19%), Postives = 514/562 (91.46%), Query Frame = 0

Query: 5   MATLPSLPGLNLFSSLPSAPPPPPPHDPTSTSVSSPSTPIPIPKYPPPKSRILRSNPP-- 64
           MA +PSLPGL+LFSSLPSAPPP   HDP+S S  +PSTPIPIPKY PPKSRILR+NPP  
Sbjct: 1   MARMPSLPGLHLFSSLPSAPPP---HDPSSAS--TPSTPIPIPKYLPPKSRILRTNPPKP 60

Query: 65  PNPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGESGVSYLLPDAPFEFQYSYSETPKVK 124
           PNPALKTFHRRSKYYKPVKDGVISSHGDRAVVIG+SGVSYLLPDAPFEFQYSYSE PKVK
Sbjct: 61  PNPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGDSGVSYLLPDAPFEFQYSYSEIPKVK 120

Query: 125 PIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVEMPGPFP 184
           PIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVEMPGPFP
Sbjct: 121 PIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVEMPGPFP 180

Query: 185 LGKYPTEAKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHSHWKRQ 244
           LGKYP E KSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHSHWKRQ
Sbjct: 181 LGKYPKEGKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHSHWKRQ 240

Query: 245 RVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFQGRNYNYRTRPQYPVMLWK 304
           RVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLF+GRNYNYRTRPQYPVMLWK
Sbjct: 241 RVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFRGRNYNYRTRPQYPVMLWK 300

Query: 305 PAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPICKLAKNGVYISLVNDVRHAFEGSIL 364
           PAAPVYPKLIQEAPEGLT EEAN+LRM+GKNLLPICKLAKNGVYISLVNDVRHAFEGSIL
Sbjct: 301 PAAPVYPKLIQEAPEGLTIEEANELRMKGKNLLPICKLAKNGVYISLVNDVRHAFEGSIL 360

Query: 365 VKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEKILMWRGKDWKSVIPEDRSAPLPLQA 424
           VK+DCTGMHESDYKKLGAKLKELVPCVLLSFDNE+ILMWRGKDWKS I ED SA LP QA
Sbjct: 361 VKVDCTGMHESDYKKLGAKLKELVPCVLLSFDNEQILMWRGKDWKSDISEDPSATLPSQA 420

Query: 425 STNNCSGSSGEPIKNSNRSHGNHHTIKTSPKMKLLWKRAIDSNKALLLNVIGLAPDDLLE 484
            TN+     GE I+N +  HGNHHTIKTSPKMKLLW++AIDSNKALLLN IGLAPDDLLE
Sbjct: 421 RTND-----GESIENGDLLHGNHHTIKTSPKMKLLWEKAIDSNKALLLNEIGLAPDDLLE 480

Query: 485 KVEEFEKISQATEHSYPAFILSSEEKVNSPDDSAESQDYSEPNYGSDDDDEDWEADL--- 544
           KVEEFE+ISQATEHSYPA I+SSE+  +SPDD+ ESQD  E NY SDDD ED E DL   
Sbjct: 481 KVEEFERISQATEHSYPALIMSSEDDSSSPDDNLESQDRDESNYSSDDD-EDREGDLFDI 540

Query: 545 -DLSAPLGSLPVDIIAKKLNPE 560
            + + P+GSLPVDIIAKKL PE
Sbjct: 541 VNPTVPMGSLPVDIIAKKLRPE 551

BLAST of MC09g1777 vs. NCBI nr
Match: KAG6601512.1 (CRS2-associated factor 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 959 bits (2479), Expect = 0.0
Identity = 489/562 (87.01%), Postives = 513/562 (91.28%), Query Frame = 0

Query: 5   MATLPSLPGLNLFSSLPSAPPPPPPHDPTSTSVSSPSTPIPIPKYPPPKSRILRSNPP-- 64
           MA +PSLPGL+LFSSLPSAPPP   HDP+S S  +PSTPIPIPKYPPPKSRILR+NPP  
Sbjct: 1   MARMPSLPGLHLFSSLPSAPPP---HDPSSAS--TPSTPIPIPKYPPPKSRILRTNPPKP 60

Query: 65  PNPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGESGVSYLLPDAPFEFQYSYSETPKVK 124
           PNPALKTFHRRSKYYKPVKDGVISSHGDRAVVIG+SGVSYLLPDAPFEFQYSYSE PKVK
Sbjct: 61  PNPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGDSGVSYLLPDAPFEFQYSYSEIPKVK 120

Query: 125 PIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVEMPGPFP 184
           PIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVEMPGPFP
Sbjct: 121 PIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVEMPGPFP 180

Query: 185 LGKYPTEAKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHSHWKRQ 244
           LGKYP E KSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHSHWKRQ
Sbjct: 181 LGKYPKEGKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHSHWKRQ 240

Query: 245 RVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFQGRNYNYRTRPQYPVMLWK 304
           RVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLF+GRNYNYRTRPQYPVMLWK
Sbjct: 241 RVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFRGRNYNYRTRPQYPVMLWK 300

Query: 305 PAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPICKLAKNGVYISLVNDVRHAFEGSIL 364
           PAAPVYPKLIQEAPEGLT EEAN+LRM+GKNLLPICKLAKNGVYISLVNDVRHAFEGSIL
Sbjct: 301 PAAPVYPKLIQEAPEGLTIEEANELRMKGKNLLPICKLAKNGVYISLVNDVRHAFEGSIL 360

Query: 365 VKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEKILMWRGKDWKSVIPEDRSAPLPLQA 424
           VK+DCTGMHESDYKKLGAKLKELVPCVLLSFDN +ILMWRGKDWKS I ED SA LP QA
Sbjct: 361 VKVDCTGMHESDYKKLGAKLKELVPCVLLSFDNAQILMWRGKDWKSDISEDPSATLPSQA 420

Query: 425 STNNCSGSSGEPIKNSNRSHGNHHTIKTSPKMKLLWKRAIDSNKALLLNVIGLAPDDLLE 484
            TN+      E I+N +  HGNHHTIKTSPKMKLLW++AIDSNKALLLN IGLAPDDLLE
Sbjct: 421 RTNDV-----ESIENGDLLHGNHHTIKTSPKMKLLWEKAIDSNKALLLNEIGLAPDDLLE 480

Query: 485 KVEEFEKISQATEHSYPAFILSSEEKVNSPDDSAESQDYSEPNYGSDDDDEDWEADL--- 544
           KVEEFE+ISQATEHSYPA I+SSE+  +SPDD+ ESQD+ E NY SDDD E  E DL   
Sbjct: 481 KVEEFERISQATEHSYPALIMSSEDDSSSPDDNLESQDHDESNYSSDDD-EYQEGDLFDN 540

Query: 545 -DLSAPLGSLPVDIIAKKLNPE 560
            + S P+GSLPVDIIAKKL PE
Sbjct: 541 VNPSVPMGSLPVDIIAKKLRPE 551

BLAST of MC09g1777 vs. ExPASy TrEMBL
Match: A0A5A7ST68 (RNA splicing factor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003020 PE=4 SV=1)

HSP 1 Score: 965 bits (2495), Expect = 0.0
Identity = 489/566 (86.40%), Postives = 526/566 (92.93%), Query Frame = 0

Query: 1   KSVTMATLPSLPGLNLFSSLPSAPPPPPPHDPTSTSVSSPSTPIPIPKYPPPKSRILRSN 60
           +SVTMAT+PSLPGL LFSSLPSAPPP   H+P+ TS  SPSTPIPIPKYPPPKSR LR+N
Sbjct: 44  RSVTMATIPSLPGLTLFSSLPSAPPP---HEPSPTS--SPSTPIPIPKYPPPKSRTLRTN 103

Query: 61  PPP---NPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGESGVSYLLPDAPFEFQYSYSE 120
            PP   NPALKTFHRRSKYYKPVKDGVISS+G+RAVVIG+SGVSYLLP APFEFQYSYSE
Sbjct: 104 NPPKSPNPALKTFHRRSKYYKPVKDGVISSNGERAVVIGDSGVSYLLPGAPFEFQYSYSE 163

Query: 121 TPKVKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVEM 180
           TP VKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQV++
Sbjct: 164 TPNVKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVQL 223

Query: 181 PGPFPLGKYPTEAKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHS 240
           PGPFPLG+YP E KSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHS
Sbjct: 224 PGPFPLGQYPKEGKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHS 283

Query: 241 HWKRQRVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFQGRNYNYRTRPQYP 300
           HWKRQRVCKVRCKGVPTVDMDNICHH+EEKTGGKIIHRVGGVLYLF+GRNYNYRTRPQYP
Sbjct: 284 HWKRQRVCKVRCKGVPTVDMDNICHHIEEKTGGKIIHRVGGVLYLFRGRNYNYRTRPQYP 343

Query: 301 VMLWKPAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPICKLAKNGVYISLVNDVRHAF 360
           VMLWKPAAPVYPKLIQEAPEGLTKEEAN+LRM+GKNLLPICKLAKNGVYISLV+DVRHAF
Sbjct: 344 VMLWKPAAPVYPKLIQEAPEGLTKEEANELRMKGKNLLPICKLAKNGVYISLVDDVRHAF 403

Query: 361 EGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEKILMWRGKDWKSVIPEDRSAP 420
           EGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNE+ILMWRGKDWKSVI +DRSAP
Sbjct: 404 EGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEQILMWRGKDWKSVISDDRSAP 463

Query: 421 LPLQASTNNCSGSSGEPIKNSNRSHGNHHTIKTSPKMKLLWKRAIDSNKALLLNVIGLAP 480
           LP +AS+N+  GSSGE ++NS+  +GNHHTIKTSPKMKLLW+RAIDSNKAL+L+ IGLAP
Sbjct: 464 LPSRASSNDSLGSSGESVENSDLLNGNHHTIKTSPKMKLLWERAIDSNKALMLDEIGLAP 523

Query: 481 DDLLEKVEEFEKISQATEHSYPAFILSSEEKVNSPDDSAESQDYSEPNYGSDDD---DED 540
           D+LLE+VEEFE+ISQATEHSYPAFI SSEE V+SP DS ESQD+SE NY SDDD   +ED
Sbjct: 524 DELLERVEEFERISQATEHSYPAFITSSEE-VSSPADSPESQDHSEANYNSDDDVGREED 583

Query: 541 WEADLDLSAPLGSLPVDIIAKKLNPE 560
              ++D   PLGSLPVDIIAKKL+ E
Sbjct: 584 LFDNVDPLVPLGSLPVDIIAKKLSSE 603

BLAST of MC09g1777 vs. ExPASy TrEMBL
Match: E5GBI3 (RNA splicing factor OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 965 bits (2495), Expect = 0.0
Identity = 489/566 (86.40%), Postives = 526/566 (92.93%), Query Frame = 0

Query: 1   KSVTMATLPSLPGLNLFSSLPSAPPPPPPHDPTSTSVSSPSTPIPIPKYPPPKSRILRSN 60
           +SVTMAT+PSLPGL LFSSLPSAPPP   H+P+ TS  SPSTPIPIPKYPPPKSR LR+N
Sbjct: 44  RSVTMATIPSLPGLTLFSSLPSAPPP---HEPSPTS--SPSTPIPIPKYPPPKSRTLRTN 103

Query: 61  PPP---NPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGESGVSYLLPDAPFEFQYSYSE 120
            PP   NPALKTFHRRSKYYKPVKDGVISS+G+RAVVIG+SGVSYLLP APFEFQYSYSE
Sbjct: 104 NPPKSPNPALKTFHRRSKYYKPVKDGVISSNGERAVVIGDSGVSYLLPGAPFEFQYSYSE 163

Query: 121 TPKVKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVEM 180
           TP VKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQV++
Sbjct: 164 TPNVKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVQL 223

Query: 181 PGPFPLGKYPTEAKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHS 240
           PGPFPLG+YP E KSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHS
Sbjct: 224 PGPFPLGQYPKEGKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHS 283

Query: 241 HWKRQRVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFQGRNYNYRTRPQYP 300
           HWKRQRVCKVRCKGVPTVDMDNICHH+EEKTGGKIIHRVGGVLYLF+GRNYNYRTRPQYP
Sbjct: 284 HWKRQRVCKVRCKGVPTVDMDNICHHIEEKTGGKIIHRVGGVLYLFRGRNYNYRTRPQYP 343

Query: 301 VMLWKPAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPICKLAKNGVYISLVNDVRHAF 360
           VMLWKPAAPVYPKLIQEAPEGLTKEEAN+LRM+GKNLLPICKLAKNGVYISLV+DVRHAF
Sbjct: 344 VMLWKPAAPVYPKLIQEAPEGLTKEEANELRMKGKNLLPICKLAKNGVYISLVDDVRHAF 403

Query: 361 EGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEKILMWRGKDWKSVIPEDRSAP 420
           EGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNE+ILMWRGKDWKSVI +DRSAP
Sbjct: 404 EGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEQILMWRGKDWKSVISDDRSAP 463

Query: 421 LPLQASTNNCSGSSGEPIKNSNRSHGNHHTIKTSPKMKLLWKRAIDSNKALLLNVIGLAP 480
           LP +AS+N+  GSSGE ++NS+  +GNHHTIKTSPKMKLLW+RAIDSNKAL+L+ IGLAP
Sbjct: 464 LPSRASSNDSLGSSGESVENSDLLNGNHHTIKTSPKMKLLWERAIDSNKALMLDEIGLAP 523

Query: 481 DDLLEKVEEFEKISQATEHSYPAFILSSEEKVNSPDDSAESQDYSEPNYGSDDD---DED 540
           D+LLE+VEEFE+ISQATEHSYPAFI SSEE V+SP DS ESQD+SE NY SDDD   +ED
Sbjct: 524 DELLERVEEFERISQATEHSYPAFITSSEE-VSSPADSPESQDHSEANYNSDDDVGREED 583

Query: 541 WEADLDLSAPLGSLPVDIIAKKLNPE 560
              ++D   PLGSLPVDIIAKKL+ E
Sbjct: 584 LFDNVDPLVPLGSLPVDIIAKKLSSE 603

BLAST of MC09g1777 vs. ExPASy TrEMBL
Match: A0A1S3BF54 (CRS2-associated factor 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103488994 PE=4 SV=1)

HSP 1 Score: 965 bits (2495), Expect = 0.0
Identity = 489/566 (86.40%), Postives = 526/566 (92.93%), Query Frame = 0

Query: 1   KSVTMATLPSLPGLNLFSSLPSAPPPPPPHDPTSTSVSSPSTPIPIPKYPPPKSRILRSN 60
           +SVTMAT+PSLPGL LFSSLPSAPPP   H+P+ TS  SPSTPIPIPKYPPPKSR LR+N
Sbjct: 52  RSVTMATIPSLPGLTLFSSLPSAPPP---HEPSPTS--SPSTPIPIPKYPPPKSRTLRTN 111

Query: 61  PPP---NPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGESGVSYLLPDAPFEFQYSYSE 120
            PP   NPALKTFHRRSKYYKPVKDGVISS+G+RAVVIG+SGVSYLLP APFEFQYSYSE
Sbjct: 112 NPPKSPNPALKTFHRRSKYYKPVKDGVISSNGERAVVIGDSGVSYLLPGAPFEFQYSYSE 171

Query: 121 TPKVKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVEM 180
           TP VKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQV++
Sbjct: 172 TPNVKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVQL 231

Query: 181 PGPFPLGKYPTEAKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHS 240
           PGPFPLG+YP E KSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHS
Sbjct: 232 PGPFPLGQYPKEGKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHS 291

Query: 241 HWKRQRVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFQGRNYNYRTRPQYP 300
           HWKRQRVCKVRCKGVPTVDMDNICHH+EEKTGGKIIHRVGGVLYLF+GRNYNYRTRPQYP
Sbjct: 292 HWKRQRVCKVRCKGVPTVDMDNICHHIEEKTGGKIIHRVGGVLYLFRGRNYNYRTRPQYP 351

Query: 301 VMLWKPAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPICKLAKNGVYISLVNDVRHAF 360
           VMLWKPAAPVYPKLIQEAPEGLTKEEAN+LRM+GKNLLPICKLAKNGVYISLV+DVRHAF
Sbjct: 352 VMLWKPAAPVYPKLIQEAPEGLTKEEANELRMKGKNLLPICKLAKNGVYISLVDDVRHAF 411

Query: 361 EGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEKILMWRGKDWKSVIPEDRSAP 420
           EGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNE+ILMWRGKDWKSVI +DRSAP
Sbjct: 412 EGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEQILMWRGKDWKSVISDDRSAP 471

Query: 421 LPLQASTNNCSGSSGEPIKNSNRSHGNHHTIKTSPKMKLLWKRAIDSNKALLLNVIGLAP 480
           LP +AS+N+  GSSGE ++NS+  +GNHHTIKTSPKMKLLW+RAIDSNKAL+L+ IGLAP
Sbjct: 472 LPSRASSNDSLGSSGESVENSDLLNGNHHTIKTSPKMKLLWERAIDSNKALMLDEIGLAP 531

Query: 481 DDLLEKVEEFEKISQATEHSYPAFILSSEEKVNSPDDSAESQDYSEPNYGSDDD---DED 540
           D+LLE+VEEFE+ISQATEHSYPAFI SSEE V+SP DS ESQD+SE NY SDDD   +ED
Sbjct: 532 DELLERVEEFERISQATEHSYPAFITSSEE-VSSPADSPESQDHSEANYNSDDDVGREED 591

Query: 541 WEADLDLSAPLGSLPVDIIAKKLNPE 560
              ++D   PLGSLPVDIIAKKL+ E
Sbjct: 592 LFDNVDPLVPLGSLPVDIIAKKLSSE 611

BLAST of MC09g1777 vs. ExPASy TrEMBL
Match: A0A6J1GXR4 (CRS2-associated factor 2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111458445 PE=4 SV=1)

HSP 1 Score: 961 bits (2483), Expect = 0.0
Identity = 490/562 (87.19%), Postives = 514/562 (91.46%), Query Frame = 0

Query: 5   MATLPSLPGLNLFSSLPSAPPPPPPHDPTSTSVSSPSTPIPIPKYPPPKSRILRSNPP-- 64
           MA +PSLPGL+LFSSLPSAPPP   HDP+S S  +PSTPIPIPKY PPKSRILR+NPP  
Sbjct: 1   MARMPSLPGLHLFSSLPSAPPP---HDPSSAS--TPSTPIPIPKYLPPKSRILRTNPPKP 60

Query: 65  PNPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGESGVSYLLPDAPFEFQYSYSETPKVK 124
           PNPALKTFHRRSKYYKPVKDGVISSHGDRAVVIG+SGVSYLLPDAPFEFQYSYSE PKVK
Sbjct: 61  PNPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGDSGVSYLLPDAPFEFQYSYSEIPKVK 120

Query: 125 PIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVEMPGPFP 184
           PIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVEMPGPFP
Sbjct: 121 PIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVEMPGPFP 180

Query: 185 LGKYPTEAKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHSHWKRQ 244
           LGKYP E KSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHSHWKRQ
Sbjct: 181 LGKYPKEGKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHSHWKRQ 240

Query: 245 RVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFQGRNYNYRTRPQYPVMLWK 304
           RVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLF+GRNYNYRTRPQYPVMLWK
Sbjct: 241 RVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFRGRNYNYRTRPQYPVMLWK 300

Query: 305 PAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPICKLAKNGVYISLVNDVRHAFEGSIL 364
           PAAPVYPKLIQEAPEGLT EEAN+LRM+GKNLLPICKLAKNGVYISLVNDVRHAFEGSIL
Sbjct: 301 PAAPVYPKLIQEAPEGLTIEEANELRMKGKNLLPICKLAKNGVYISLVNDVRHAFEGSIL 360

Query: 365 VKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEKILMWRGKDWKSVIPEDRSAPLPLQA 424
           VK+DCTGMHESDYKKLGAKLKELVPCVLLSFDNE+ILMWRGKDWKS I ED SA LP QA
Sbjct: 361 VKVDCTGMHESDYKKLGAKLKELVPCVLLSFDNEQILMWRGKDWKSDISEDPSATLPSQA 420

Query: 425 STNNCSGSSGEPIKNSNRSHGNHHTIKTSPKMKLLWKRAIDSNKALLLNVIGLAPDDLLE 484
            TN+     GE I+N +  HGNHHTIKTSPKMKLLW++AIDSNKALLLN IGLAPDDLLE
Sbjct: 421 RTND-----GESIENGDLLHGNHHTIKTSPKMKLLWEKAIDSNKALLLNEIGLAPDDLLE 480

Query: 485 KVEEFEKISQATEHSYPAFILSSEEKVNSPDDSAESQDYSEPNYGSDDDDEDWEADL--- 544
           KVEEFE+ISQATEHSYPA I+SSE+  +SPDD+ ESQD  E NY SDDD ED E DL   
Sbjct: 481 KVEEFERISQATEHSYPALIMSSEDDSSSPDDNLESQDRDESNYSSDDD-EDREGDLFDI 540

Query: 545 -DLSAPLGSLPVDIIAKKLNPE 560
            + + P+GSLPVDIIAKKL PE
Sbjct: 541 VNPTVPMGSLPVDIIAKKLRPE 551

BLAST of MC09g1777 vs. ExPASy TrEMBL
Match: A0A5D3CUP7 (RNA splicing factor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001140 PE=4 SV=1)

HSP 1 Score: 959 bits (2479), Expect = 0.0
Identity = 486/562 (86.48%), Postives = 522/562 (92.88%), Query Frame = 0

Query: 5   MATLPSLPGLNLFSSLPSAPPPPPPHDPTSTSVSSPSTPIPIPKYPPPKSRILRSNPPP- 64
           MAT+PSLPGL LFSSLPSAPPP   H+P+ TS  SPSTPIPIPKYPPPKSR LR+N PP 
Sbjct: 1   MATIPSLPGLTLFSSLPSAPPP---HEPSPTS--SPSTPIPIPKYPPPKSRTLRTNNPPK 60

Query: 65  --NPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGESGVSYLLPDAPFEFQYSYSETPKV 124
             NPALKTFHRRSKYYKPVKDGVISS+G+RAVVIG+SGVSYLLP APFEFQYSYSETP V
Sbjct: 61  SPNPALKTFHRRSKYYKPVKDGVISSNGERAVVIGDSGVSYLLPGAPFEFQYSYSETPNV 120

Query: 125 KPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVEMPGPF 184
           KPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQV++PGPF
Sbjct: 121 KPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQVQLPGPF 180

Query: 185 PLGKYPTEAKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHSHWKR 244
           PLG+YP E KSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHSHWKR
Sbjct: 181 PLGQYPKEGKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLELIHSHWKR 240

Query: 245 QRVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFQGRNYNYRTRPQYPVMLW 304
           QRVCKVRCKGVPTVDMDNICHH+EEKTGGKIIHRVGGVLYLF+GRNYNYRTRPQYPVMLW
Sbjct: 241 QRVCKVRCKGVPTVDMDNICHHIEEKTGGKIIHRVGGVLYLFRGRNYNYRTRPQYPVMLW 300

Query: 305 KPAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPICKLAKNGVYISLVNDVRHAFEGSI 364
           KPAAPVYPKLIQEAPEGLTKEEAN+LRM+GKNLLPICKLAKNGVYISLV+DVRHAFEGSI
Sbjct: 301 KPAAPVYPKLIQEAPEGLTKEEANELRMKGKNLLPICKLAKNGVYISLVDDVRHAFEGSI 360

Query: 365 LVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEKILMWRGKDWKSVIPEDRSAPLPLQ 424
           LVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNE+ILMWRGKDWKSVI +DRSAPLP +
Sbjct: 361 LVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEQILMWRGKDWKSVISDDRSAPLPSR 420

Query: 425 ASTNNCSGSSGEPIKNSNRSHGNHHTIKTSPKMKLLWKRAIDSNKALLLNVIGLAPDDLL 484
           AS+N+  GSSGE ++NS+  +GNHHTIKTSPKMKLLW+RAIDSNKAL+L+ IGLAPD+LL
Sbjct: 421 ASSNDSLGSSGESVENSDLLNGNHHTIKTSPKMKLLWERAIDSNKALMLDEIGLAPDELL 480

Query: 485 EKVEEFEKISQATEHSYPAFILSSEEKVNSPDDSAESQDYSEPNYGSDDD---DEDWEAD 544
           E+VEEFE+ISQATEHSYPAFI SSEE V+SP DS ESQD+SE NY SDDD   +ED   +
Sbjct: 481 ERVEEFERISQATEHSYPAFITSSEE-VSSPADSPESQDHSEANYNSDDDVGREEDLFDN 540

Query: 545 LDLSAPLGSLPVDIIAKKLNPE 560
           +D   PLGSLPVDIIAKKL+ E
Sbjct: 541 VDPLVPLGSLPVDIIAKKLSSE 556

BLAST of MC09g1777 vs. TAIR 10
Match: AT1G23400.1 (RNA-binding CRS1 / YhbY (CRM) domain-containing protein )

HSP 1 Score: 693.7 bits (1789), Expect = 1.2e-199
Identity = 361/567 (63.67%), Postives = 432/567 (76.19%), Query Frame = 0

Query: 5   MATLPSLPGLNLFSSLPSAPPPPPPHDPTSTSVSSPSTPIPIPKYPPP-KSRILRSN--- 64
           MA + SL  +NLFSSLPS PP       +S+    P+ PIPIPKY P  ++R  ++N   
Sbjct: 1   MAIVASLRDINLFSSLPSTPPMA----DSSSGTFRPAPPIPIPKYAPSNRNRNQKTNHQT 60

Query: 65  ------PPPNPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGESGVSYLLPDAPFEFQYS 124
                 P  NPALK  H R++YYKPVK+GVISS GDR ++IG+SGVSY LP APFEFQ+S
Sbjct: 61  DTNPKKPQSNPALKLPHHRTRYYKPVKEGVISSDGDRTILIGDSGVSYQLPGAPFEFQFS 120

Query: 125 YSETPKVKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPGTKGVKQ 184
           YSETPKVKP+ IREPAF+PFAPPTMPRPWTGKAPLK SKKKIPLFDSFNPPP G  GVK 
Sbjct: 121 YSETPKVKPVGIREPAFMPFAPPTMPRPWTGKAPLKKSKKKIPLFDSFNPPPAGKSGVKY 180

Query: 185 VEMPGPFPLGKYPTEAKSREEILGEPLKNWEIRMLVKPHLSHNRQVNLGRDGLTHNMLEL 244
           VEMPGP P G+YP E  +REE+LGEPLK WE  ML+KPH+  NRQVNLGRDG THNMLEL
Sbjct: 181 VEMPGPLPFGRYPKEGMNREEVLGEPLKRWEKGMLIKPHMHDNRQVNLGRDGFTHNMLEL 240

Query: 245 IHSHWKRQRVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFQGRNYNYRTRP 304
           IHSHWKR+RVCKVRCKGVPTVDM+N+C  LEEKTGG+IIHRVGGV+YLF+GRNYNYRTRP
Sbjct: 241 IHSHWKRRRVCKVRCKGVPTVDMNNVCRVLEEKTGGEIIHRVGGVVYLFRGRNYNYRTRP 300

Query: 305 QYPVMLWKPAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPICKLAKNGVYISLVNDVR 364
           QYP+MLWKPAAPVYPKLIQE PEGLTKEEA++ R++GK+L PICKL+KNGVY+SLV DVR
Sbjct: 301 QYPLMLWKPAAPVYPKLIQEVPEGLTKEEAHEFRVKGKSLRPICKLSKNGVYVSLVKDVR 360

Query: 365 HAFEGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEKILMWRGKDWKSVIPEDR 424
            AFE S LVK+DC G+  SDYKK+GAKLKELVPCVLLSFD+E+ILMWRG++WKS   ++ 
Sbjct: 361 DAFELSSLVKVDCPGLEPSDYKKIGAKLKELVPCVLLSFDDEQILMWRGREWKSRFVDNP 420

Query: 425 SAPLPLQASTNNCSGSSGEPIKNSNRSHGNHHTIKTSPKMKLLWKRAIDSNKALLLNVIG 484
             P   + +T N    S +P  +  ++  N  T  +SPKM  LW+RA++S+KA++L  + 
Sbjct: 421 LIPSLSETNTTNELDPSDKP--SEEQTVANPSTTISSPKMISLWQRALESSKAVILEELD 480

Query: 485 LAPDDLLEKVEEFEKISQATEHSYPAFILSSEEKVNSPDDSAESQDYSEPNYGSDDDDED 544
           L PDDLL+KVEE E  S A EH+Y A +LS+ +   + +D  + +D SE  Y   DDD D
Sbjct: 481 LGPDDLLKKVEELEGTSLAAEHTYTAMVLSNTD--GAAEDYVDEKDRSEEYYSDIDDDFD 540

Query: 545 WEA----DLDLSAPLGSLPVDIIAKKL 558
            E      LD   P+GSLPVD I +KL
Sbjct: 541 DECSDDESLDPVGPVGSLPVDKIVRKL 559

BLAST of MC09g1777 vs. TAIR 10
Match: AT2G20020.1 (RNA-binding CRS1 / YhbY (CRM) domain-containing protein )

HSP 1 Score: 432.2 bits (1110), Expect = 6.5e-121
Identity = 230/481 (47.82%), Postives = 307/481 (63.83%), Query Frame = 0

Query: 52  PKSRILRSNPPPN-PALKTF-HRRSKYYKP------------VKDGVISSHGDRAVVIGE 111
           PK+   RS   P+ P+ ++   RRSKY KP            V D          V + E
Sbjct: 80  PKTSPFRSRGTPSLPSARSIPGRRSKYSKPDSGPNRPKNKPRVPDSPPQLDAKPEVKLSE 139

Query: 112 SGVSYLLPDAPFEFQYSYSETPKVKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIP 171
            G++Y++  APFEF+YSY+ETPKVKP+ +REPA+ PF P TM RPWTG+APL  S+K   
Sbjct: 140 DGLTYVINGAPFEFKYSYTETPKVKPLKLREPAYAPFGPTTMGRPWTGRAPLPQSQKTPR 199

Query: 172 LFDSFNPPPPGTKGVKQVEMPGPFPLGKYPTEAKSREEILGEPLKNWEIRMLVKPHLSHN 231
            FDSF  PP G KG+K V+ PGPF  G  P    S+EEILGEPL   E+R LV   L   
Sbjct: 200 EFDSFRLPPVGKKGLKPVQKPGPFRPGVGPRYVYSKEEILGEPLTKEEVRELVTSCLKTT 259

Query: 232 RQVNLGRDGLTHNMLELIHSHWKRQRVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVG 291
           RQ+N+GRDGLTHNML  IH  WKR+RVCK++CKGV TVDMDN+C  LEEK GGK+I+R G
Sbjct: 260 RQLNMGRDGLTHNMLNNIHDLWKRRRVCKIKCKGVCTVDMDNVCEQLEEKIGGKVIYRRG 319

Query: 292 GVLYLFQGRNYNYRTRPQYPVMLWKPAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPI 351
           GVL+LF+GRNYN+RTRP++P+MLWKP APVYP+LIQ+ PEGLT++EA  +R +G+ L+PI
Sbjct: 320 GVLFLFRGRNYNHRTRPRFPLMLWKPVAPVYPRLIQQVPEGLTRQEATNMRRKGRELMPI 379

Query: 352 CKLAKNGVYISLVNDVRHAFEGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEK 411
           CKL KNGVY  LV +V+ AFE   LV+IDC GM  SD++K+GAKLK+LVPCVL+SF+NE+
Sbjct: 380 CKLGKNGVYCDLVKNVKEAFEVCELVRIDCQGMKGSDFRKIGAKLKDLVPCVLVSFENEQ 439

Query: 412 ILMWRGKDWK-SVIPEDRSAPLPLQASTNNCSGSSGEPIKNSNRSHGNHHTIKTSPKMKL 471
           IL+WRG++WK S+   D+   +      +       EP  + N+S     T+  +P +  
Sbjct: 440 ILIWRGREWKSSLTTPDKKGDILEDIEVDTALPEDDEPSVSPNQS----QTMTQNPPLDS 499

Query: 472 LWKRAIDSNKALLLNVIGLAPDDLLEKVEEFEKISQATEHSYPAFILSSEEKVNSPDDSA 518
           +  +       L  + +  +  +      +       TE +  +F+   EE  + P+ S 
Sbjct: 500 MELQNDPDGHDLSPSTVDSSEMEGTINSLQSWSTKDVTEPTVDSFLRDLEEPEDEPETSE 556

BLAST of MC09g1777 vs. TAIR 10
Match: AT4G31010.1 (RNA-binding CRS1 / YhbY (CRM) domain-containing protein )

HSP 1 Score: 265.4 bits (677), Expect = 1.0e-70
Identity = 147/367 (40.05%), Postives = 207/367 (56.40%), Query Frame = 0

Query: 50  PPPKSRILRSNPPPNPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGESGVSYLLPDAPF 109
           PPP S     NP  N       +    Y+P                   GV  +  D PF
Sbjct: 40  PPPLSSSASENPDFNQKNNNKKKPKPQYRPPS--------------SLEGVKTVHSDLPF 99

Query: 110 EFQYSYSET-PKVKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPG 169
           +F++SY+E+   V+PI +REP + PF P  + R WTG               + NP    
Sbjct: 100 DFRFSYTESCSNVRPIGLREPKYSPFGPDRLDREWTGVCA-----------PAVNPKVES 159

Query: 170 TKGVKQVEMPGPFPLGKYPTEAKSREEILGEPLKNWEIRMLVK--PHLSHNRQVNLGRDG 229
             GV+  ++             K RE+I G  L   E + LV+        RQVNLGRDG
Sbjct: 160 VDGVEDPKLE--------EKRRKVREKIQGASLTEAERKFLVELCQRNKTKRQVNLGRDG 219

Query: 230 LTHNMLELIHSHWKRQRVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFQGR 289
           LTHNML  +++HWK     +V+C GVPT+DM N+  HLE+KT G+++ +  G L L++GR
Sbjct: 220 LTHNMLNDVYNHWKHAEAVRVKCLGVPTLDMKNVIFHLEDKTFGQVVSKHSGTLVLYRGR 279

Query: 290 NYNYRTRPQYPVMLWKPAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPICKLAKNGVY 349
           NY+ + RP+ P+MLWKP  PVYP+LI+   +GL+ +E   +R +G  +  + KLAKNG Y
Sbjct: 280 NYDPKKRPKIPLMLWKPHEPVYPRLIKTTIDGLSIDETKAMRKKGLAVPALTKLAKNGYY 339

Query: 350 ISLVNDVRHAFEGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEKILMWRGKDW 409
            SLV  VR AF  S LV+IDC G+   DYKK+GAKL++LVPC+L++FD E++++WRGKD+
Sbjct: 340 GSLVPMVRDAFLVSELVRIDCLGLERKDYKKIGAKLRDLVPCILVTFDKEQVVIWRGKDY 373

Query: 410 KSVIPED 414
           K    +D
Sbjct: 400 KPPKEDD 373

BLAST of MC09g1777 vs. TAIR 10
Match: AT5G54890.1 (RNA-binding CRS1 / YhbY (CRM) domain-containing protein )

HSP 1 Score: 262.3 bits (669), Expect = 8.8e-70
Identity = 129/306 (42.16%), Postives = 192/306 (62.75%), Query Frame = 0

Query: 103 LLPDAPFEFQYSYSET-PKVKPIAIREP-AFLPFAPPTMPRPWTGKAPLKSSKKKIPLFD 162
           ++ D PF+F+YSYSET P+++PI  REP  F PF P  + R WTG   L S +       
Sbjct: 73  VISDLPFDFRYSYSETNPEIEPIGFREPKRFSPFGPGRLDRKWTGTTALASPE------- 132

Query: 163 SFNPPPPGTKGVKQVEMPGPFPLGKYPTEAKSREEILGEPLKNWEIRMLVK--PHLSHNR 222
                      + Q +              + R  +LGE L   E+  L++   H    R
Sbjct: 133 -----------IDQSQW------------VEERARVLGETLTEDEVTELIERYRHSDCTR 192

Query: 223 QVNLGRDGLTHNMLELIHSHWKRQRVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGG 282
           Q+NLG+ G+THNM++ IH+HWK+    +++C GVPT+DMDNIC HLEEK+GGKI++R   
Sbjct: 193 QINLGKGGVTHNMIDDIHNHWKKAEAVRIKCLGVPTLDMDNICFHLEEKSGGKIVYRNIN 252

Query: 283 VLYLFQGRNYNYRTRPQYPVMLWKPAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPIC 342
           +L L++GRNY+ ++RP  P+MLWKP  P+YP+L++   +GL  EE  ++R +G +   + 
Sbjct: 253 ILVLYRGRNYDPKSRPIIPLMLWKPHPPIYPRLVKNVADGLEFEETKEMRNRGLHSPALM 312

Query: 343 KLAKNGVYISLVNDVRHAFEGSILVKIDCTGMHESDYKKLGAKLKELVPCVLLSFDNEKI 402
           KL +NGVY+++V  VR  FE   +V++DCT +  SD K++G KLKE+VPCV + F +E+I
Sbjct: 313 KLTRNGVYVNVVGRVREEFETEEIVRLDCTHVGMSDCKRIGVKLKEMVPCVPILFKDEQI 348

Query: 403 LMWRGK 405
           ++WRGK
Sbjct: 373 ILWRGK 348

BLAST of MC09g1777 vs. TAIR 10
Match: AT4G31010.2 (RNA-binding CRS1 / YhbY (CRM) domain-containing protein )

HSP 1 Score: 177.2 bits (448), Expect = 3.7e-44
Identity = 108/301 (35.88%), Postives = 154/301 (51.16%), Query Frame = 0

Query: 50  PPPKSRILRSNPPPNPALKTFHRRSKYYKPVKDGVISSHGDRAVVIGESGVSYLLPDAPF 109
           PPP S     NP  N       +    Y+P                   GV  +  D PF
Sbjct: 40  PPPLSSSASENPDFNQKNNNKKKPKPQYRPPS--------------SLEGVKTVHSDLPF 99

Query: 110 EFQYSYSET-PKVKPIAIREPAFLPFAPPTMPRPWTGKAPLKSSKKKIPLFDSFNPPPPG 169
           +F++SY+E+   V+PI +REP + PF P  + R WTG               + NP    
Sbjct: 100 DFRFSYTESCSNVRPIGLREPKYSPFGPDRLDREWTGVCA-----------PAVNPKVES 159

Query: 170 TKGVKQVEMPGPFPLGKYPTEAKSREEILGEPLKNWEIRMLVK--PHLSHNRQVNLGRDG 229
             GV+  ++             K RE+I G  L   E + LV+        RQVNLGRDG
Sbjct: 160 VDGVEDPKLE--------EKRRKVREKIQGASLTEAERKFLVELCQRNKTKRQVNLGRDG 219

Query: 230 LTHNMLELIHSHWKRQRVCKVRCKGVPTVDMDNICHHLEEKTGGKIIHRVGGVLYLFQGR 289
           LTHNML  +++HWK     +V+C GVPT+DM N+  HLE+KT G+++ +  G L L++GR
Sbjct: 220 LTHNMLNDVYNHWKHAEAVRVKCLGVPTLDMKNVIFHLEDKTFGQVVSKHSGTLVLYRGR 279

Query: 290 NYNYRTRPQYPVMLWKPAAPVYPKLIQEAPEGLTKEEANKLRMQGKNLLPICKLAKNGVY 348
           NY+ + RP+ P+MLWKP  PVYP+LI+   +GL+ +E   +R +G   L +  L K G Y
Sbjct: 280 NYDPKKRPKIPLMLWKPHEPVYPRLIKTTIDGLSIDETKAMRKKG---LAVPALTKLGPY 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LDA91.7e-19863.67CRS2-associated factor 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g2... [more]
Q657G77.1e-14950.91CRS2-associated factor 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947... [more]
Q84N482.4e-14450.25CRS2-associated factor 2, chloroplastic OS=Zea mays OX=4577 GN=CAF2 PE=1 SV=1[more]
Q5VMQ53.0e-12355.25CRS2-associated factor 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947... [more]
Q84N491.7e-12157.85CRS2-associated factor 1, chloroplastic OS=Zea mays OX=4577 GN=CAF1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038892001.10.087.39CRS2-associated factor 2, chloroplastic [Benincasa hispida][more]
XP_008446191.10.086.40PREDICTED: CRS2-associated factor 2, chloroplastic [Cucumis melo][more]
ADN33825.10.086.40RNA splicing factor [Cucumis melo subsp. melo] >KAA0034302.1 RNA splicing factor... [more]
XP_022956896.10.087.19CRS2-associated factor 2, chloroplastic [Cucurbita moschata][more]
KAG6601512.10.087.01CRS2-associated factor 2, chloroplastic, partial [Cucurbita argyrosperma subsp. ... [more]
Match NameE-valueIdentityDescription
A0A5A7ST680.086.40RNA splicing factor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G0... [more]
E5GBI30.086.40RNA splicing factor OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A1S3BF540.086.40CRS2-associated factor 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103488994 ... [more]
A0A6J1GXR40.087.19CRS2-associated factor 2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A5D3CUP70.086.48RNA splicing factor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G0... [more]
Match NameE-valueIdentityDescription
AT1G23400.11.2e-19963.67RNA-binding CRS1 / YhbY (CRM) domain-containing protein [more]
AT2G20020.16.5e-12147.82RNA-binding CRS1 / YhbY (CRM) domain-containing protein [more]
AT4G31010.11.0e-7040.05RNA-binding CRS1 / YhbY (CRM) domain-containing protein [more]
AT5G54890.18.8e-7042.16RNA-binding CRS1 / YhbY (CRM) domain-containing protein [more]
AT4G31010.23.7e-4435.88RNA-binding CRS1 / YhbY (CRM) domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001890RNA-binding, CRM domainSMARTSM01103CRS1_YhbY_2coord: 319..402
e-value: 5.5E-9
score: 45.9
coord: 201..284
e-value: 1.0E-16
score: 71.5
IPR001890RNA-binding, CRM domainPFAMPF01985CRS1_YhbYcoord: 202..283
e-value: 5.6E-19
score: 68.1
coord: 319..402
e-value: 6.1E-11
score: 42.3
IPR001890RNA-binding, CRM domainPROSITEPS51295CRMcoord: 317..413
score: 15.652727
IPR001890RNA-binding, CRM domainPROSITEPS51295CRMcoord: 199..295
score: 17.554186
IPR035920YhbY-like superfamilyGENE3D3.30.110.60coord: 200..296
e-value: 3.1E-19
score: 70.8
IPR035920YhbY-like superfamilyGENE3D3.30.110.60coord: 319..415
e-value: 8.3E-16
score: 59.8
IPR035920YhbY-like superfamilySUPERFAMILY75471YhbY-likecoord: 319..409
IPR035920YhbY-like superfamilySUPERFAMILY75471YhbY-likecoord: 200..293
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 418..438
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 43..59
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..70
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 417..448
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..17
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 506..542
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 524..539
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 19..34
NoneNo IPR availablePANTHERPTHR46247:SF3CRS2-ASSOCIATED FACTOR 2, CHLOROPLASTICcoord: 5..558
IPR044599CRS2-associated factor, plantPANTHERPTHR46247CRS2-ASSOCIATED FACTOR 1, CHLOROPLASTICcoord: 5..558

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g1777.1MC09g1777.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000373 Group II intron splicing
biological_process GO:0006397 mRNA processing
molecular_function GO:0003729 mRNA binding
molecular_function GO:0003723 RNA binding