MC09g1451 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC09g1451
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like
Location	MC09: 20501159 .. 20503329 (+)
RNA-Seq Expression	MC09g1451
Synteny	MC09g1451

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGGCGGCGATGGACATTTCGTCGGCGTTTCTTTTCCCGCCGCCTCCTTCGACGTTTGTGAACGTGATGACAGTGGTGAGTTCGACGGCGATGGCGATGTCCGGATTTTCCGAGATGAACGGAAAGCATTTGCAGTATTCCAAGTTCTGGAACGCGAATGGTGCTTCGAAATCTTCATCGGAGGAATCTAAGGTTTCGTCGAAATACGGAATGCTCTTGGCTTATACTCCGGCGTTTCTCGCCGGAGCGGCGTCGTTTTGGTTCTTTCCGAGCGACGATGGAAGGATTCTTCTCCTCAAATCTGCTCTCACCTTCCATTTCTTCAAGAGGATCTTTGAGGTACTCTCTCTCCCGCTCTACGCCTCTGTTTTAGTATAGTGCACTTCCTTCTCGATTCGTGATTAGGTATTCATATTCTCGTGAAATTAGTTCGATTCATTTTGGACTCCAGTAAGAATCGGGGCTTCCTTGAGAATCGGTTAATCTAATTCTTCGAAATTTTGTCGAGTTCTTGAAAATGGTGGAGAGATCCGCATCTCTTGTCCCTTTCCATTGCTCTGTACATCTTATCTTATCGTTCCTCTGGAATTTCCAAGTTTGGGACCACCGTTTTTGTTTGTTTGTTTTTCTTCCTTTTACGATAATAGGGAGATTATGTCAAAAATGTGTAGAAATTTTATATGTCGTATGAACTTGGCTAATTAATTAGACGATCAGCTCATTAATGTCTGGATTCATTAATTTTTCTTTTTGACTATTGAATTGACATTAATACTCTCTTCGAGAACGGCGTATTTTAGAATGATAATTGCTATATTATTGTTATATGAAGTCATGTTTAAGAAACAAATTACTAGGATTCTGTTTAATACTGATTTTGTGATGCTTCGCACTTGTACTATTCATTCCTGGCACATATATTGTTTTGCAAACATTTTGAAGGTTCTTCTCAAATTATAGTAGCTATTTAGAGAACAACAATACTTGGTAGCAACCCAATCTTCATGTTGTCAATTATTCAAAGTTTCACAGCTCTTTGATTTTTTGAAAAAAAAAATCTATCTTTTTATATTCCCTTGTAAAAATTTAATGGTCACCGAGAGGTAGAGGTAAGTACCTAAGTATTATTTAATAAACTTCTTTAGATTTAGAGAATATAAACTTCTTTAGATTTAGTTATTCTCTAAAGAATAATACTTAGGAAAATATTTGGTTCTTTAGGAGAAATATTGTCCTTACCATCCACTGAATGTTTAACAACTAAGAGACAGGATGCAAAAACAGAATTTTTTTATGAAAATTAATGAGTTGCCTTGGTGAGGACAAGATGAAGATAAGATTTGGTCATCATAGTCAAGGAACCAAATATTTTTCTAATACTTTAAGTCATCAGCTTGATAAATAGAGAGAGAAGTGCAGAAAAGAGATATTTCAAAAATCAAAGACATGTCAAATTTTGAGCGGTAGTTCTAAGAATAATATTACCTATCTCTTACTGAATAATATTACTATTCACCTGTTCACCATTAAATCTTTAATAAGTGGAGAGAGGAATGCAGAAAGAGCTAACTTTTCCTTCTCAGAAAATTAAACAACCATCAAATTTTGAGTAAGCAACAAAAATCTTTCTTGAATGTAGCAAAACAAATTCTCAAACAGTCCCTAACAGACTCTTCTCCTATTTGAAAATTCTATGCTCACAGGTACTGTTTGTTCACAAGTACAGCAGCAAAATGGTATTGGATACAGCATTCAAAATCTCTCTCAGCTACTTCCTCTCCACAGCAATCATGATCTACACTCAGCACCTCTCACAAGGGTTTCCAGCGCCATCCATTGATCTGAAAAACATTGGAATTGGTTGCTTTGTGATGGGCATTATTGGGAACTTTTACCACCACTATCTGTTGTCCAGAACCAGAGGAAAGGGGGAGAAAGAGTATAAGATTCCCAATGGAGGTCTTTTCTGCCTTGTCATCTGCCCTCACTATCTGTTTGAAATAATTGTGTATTTTGGGTTTGCTTTCATCTCTCAGGCAATCTTCCCACTGTGCTTCGCCACTGGCACTGCTTTGTACTTGGCCGGGAGGAGCTATGCCACTAGGAAATGGTATGTCTCCAAATTTGAACACTTCCCCAACCATGTCAAAGCCCTACTACCTTTTCTCTTT

mRNA sequence

GCGGCGGCGATGGACATTTCGTCGGCGTTTCTTTTCCCGCCGCCTCCTTCGACGTTTGTGAACGTGATGACAGTGGTGAGTTCGACGGCGATGGCGATGTCCGGATTTTCCGAGATGAACGGAAAGCATTTGCAGTATTCCAAGTTCTGGAACGCGAATGGTGCTTCGAAATCTTCATCGGAGGAATCTAAGGTTTCGTCGAAATACGGAATGCTCTTGGCTTATACTCCGGCGTTTCTCGCCGGAGCGGCGTCGTTTTGGTTCTTTCCGAGCGACGATGGAAGGATTCTTCTCCTCAAATCTGCTCTCACCTTCCATTTCTTCAAGAGGATCTTTGAGGTACTGTTTGTTCACAAGTACAGCAGCAAAATGGTATTGGATACAGCATTCAAAATCTCTCTCAGCTACTTCCTCTCCACAGCAATCATGATCTACACTCAGCACCTCTCACAAGGGTTTCCAGCGCCATCCATTGATCTGAAAAACATTGGAATTGGTTGCTTTGTGATGGGCATTATTGGGAACTTTTACCACCACTATCTGTTGTCCAGAACCAGAGGAAAGGGGGAGAAAGAGTATAAGATTCCCAATGGAGGTCTTTTCTGCCTTGTCATCTGCCCTCACTATCTGTTTGAAATAATTGTGTATTTTGGGTTTGCTTTCATCTCTCAGGCAATCTTCCCACTGTGCTTCGCCACTGGCACTGCTTTGTACTTGGCCGGGAGGAGCTATGCCACTAGGAAATGGTATGTCTCCAAATTTGAACACTTCCCCAACCATGTCAAAGCCCTACTACCTTTTCTCTTT

Coding sequence (CDS)

GCGGCGGCGATGGACATTTCGTCGGCGTTTCTTTTCCCGCCGCCTCCTTCGACGTTTGTGAACGTGATGACAGTGGTGAGTTCGACGGCGATGGCGATGTCCGGATTTTCCGAGATGAACGGAAAGCATTTGCAGTATTCCAAGTTCTGGAACGCGAATGGTGCTTCGAAATCTTCATCGGAGGAATCTAAGGTTTCGTCGAAATACGGAATGCTCTTGGCTTATACTCCGGCGTTTCTCGCCGGAGCGGCGTCGTTTTGGTTCTTTCCGAGCGACGATGGAAGGATTCTTCTCCTCAAATCTGCTCTCACCTTCCATTTCTTCAAGAGGATCTTTGAGGTACTGTTTGTTCACAAGTACAGCAGCAAAATGGTATTGGATACAGCATTCAAAATCTCTCTCAGCTACTTCCTCTCCACAGCAATCATGATCTACACTCAGCACCTCTCACAAGGGTTTCCAGCGCCATCCATTGATCTGAAAAACATTGGAATTGGTTGCTTTGTGATGGGCATTATTGGGAACTTTTACCACCACTATCTGTTGTCCAGAACCAGAGGAAAGGGGGAGAAAGAGTATAAGATTCCCAATGGAGGTCTTTTCTGCCTTGTCATCTGCCCTCACTATCTGTTTGAAATAATTGTGTATTTTGGGTTTGCTTTCATCTCTCAGGCAATCTTCCCACTGTGCTTCGCCACTGGCACTGCTTTGTACTTGGCCGGGAGGAGCTATGCCACTAGGAAATGGTATGTCTCCAAATTTGAACACTTCCCCAACCATGTCAAAGCCCTACTACCTTTTCTCTTT

Protein sequence

AAAMDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEESKVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKYSSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF

Homology

[Close]

BLAST of MC09g1451 vs. ExPASy Swiss-Prot
Match: Q55C17 (Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn2 PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.4e-14
Identity = 57/170 (33.53%), Postives = 88/170 (51.76%), Query Frame = 0


Query: 104 TFHFFKRIFEVLFVHKYS-SKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKN 163
           + H+ KRI+E +FVH++S   M +   FK    Y+  TA++ Y  +      AP I+   
Sbjct: 134 SLHYIKRIYETIFVHRFSHGTMPIFNLFKNCSYYWGCTAMVSYFVNHPLYTEAP-IERVY 193

Query: 164 IGIGCFVMGIIGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFI 223
           +G+G +++G + N+  H  L   R  G  E KIP G LF  V CP+Y  EI+ + GF+ +
Sbjct: 194 LGLGLWIIGEVFNYICHIQLRNLRPAGSTERKIPRGLLFEFVSCPNYTVEILSWIGFSIL 253

Query: 224 SQA----IFPLCFATGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFL 269
           +Q     IF L  A    ++  G+    RK +  K   +P   K L+PFL
Sbjct: 254 TQTLTSWIFALMGAAQMWIWAVGKHRRYRKEFGDK---YPKSRKILIPFL 299

[Close]

BLAST of MC09g1451 vs. ExPASy Swiss-Prot
Match: O18765 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Sus scrofa OX=9823 GN=SRD5A2 PE=2 SV=2)

HSP 1 Score: 77.4 bits (189), Expect = 2.7e-13
Identity = 40/108 (37.04%), Postives = 65/108 (60.19%), Query Frame = 0


Query: 162 NIGIGCFVMGIIGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAF 221
           ++G+  F++G+  N +  Y+L + R  GE  YKIP GGLF  V   ++L EII + G+A 
Sbjct: 147 SLGVFLFILGMGINIHSDYILRQLRKPGEVIYKIPQGGLFTYVSGANFLGEIIEWIGYAL 206

Query: 222 ISQAIFPLCFATGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 270
            + ++  L FA  +  +L  R++   ++YV  FE +P   KAL+PF+F
Sbjct: 207 ATWSLPALAFAFFSLCFLGLRAFHHHRFYVKMFEDYPKSRKALIPFIF 254

[Close]

BLAST of MC09g1451 vs. ExPASy Swiss-Prot
Match: Q28892 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis OX=9541 GN=SRD5A2 PE=1 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.0e-12
Identity = 49/168 (29.17%), Postives = 89/168 (52.98%), Query Frame = 0


Query: 106 HFFKRIFEVLFVHK---YSSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLK- 165
           H+F R F    +++   Y + ++       + + FL +  +IY      G+     D++ 
Sbjct: 90  HYFHRTFVYSLLNRGRPYPAVLIFRGIAFCAGNGFLQSYYLIYCAEYPDGW---YTDIRF 149

Query: 166 NIGIGCFVMGIIGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAF 225
            +G+  F++G+  N +  Y+L + R  GE  Y+IP GGLF  V   ++L EII + G+A 
Sbjct: 150 CLGVFLFILGMGVNIHSDYILRQLRKPGEITYRIPKGGLFTYVSGANFLGEIIEWIGYAL 209

Query: 226 ISQAIFPLCFATGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 270
            + ++  L FA  +  +L  R++   ++Y+  FE +P   KAL+PF+F
Sbjct: 210 ATWSLPALAFAFFSVCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF 254

[Close]

BLAST of MC09g1451 vs. ExPASy Swiss-Prot
Match: Q2QDF6 (Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.8e-12
Identity = 60/200 (30.00%), Postives = 100/200 (50.00%), Query Frame = 0


Query: 85  SFWFFPSD----DGRILLLKSALTFHFFKRIFEVLFVHKYSSKMVLDTAFKISLSYFLST 144
           +F+ FPS     + +  LL S   FH+F R   VL+  + +        F +S++ F++ 
Sbjct: 62  TFFLFPSGQHFYNPKSFLLISPFLFHYFNR--TVLYPLRLARNTTQTRGFPVSVA-FMAF 121

Query: 145 AIMIYTQHLSQGFPAP-SIDLKN---------IGIGCFVMGIIGNFYHHYLLSRTRGKGE 204
              +   +L   + +    D +N          G+  FV+G+  N     +L   + +G+
Sbjct: 122 GFNLLNGYLQARWVSHYKDDYENEELFWWRFLAGLLIFVVGMWVNVRADKVLVGLKKQGD 181

Query: 205 KEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRSYATRKWY 264
             YKIP GGLF LV CP+Y  EI+ +FG+A ++ +     F   T   L  R+ ATR WY
Sbjct: 182 GGYKIPRGGLFELVSCPNYFGEIMEWFGWAVMTWSWVGFGFFLYTCANLMPRARATRLWY 241

Query: 265 VSKF-EHFPNHVKALLPFLF 270
           + KF + +P   KA++PF++
Sbjct: 242 LEKFKDDYPKDRKAVIPFIY 258

[Close]

BLAST of MC09g1451 vs. ExPASy Swiss-Prot
Match: P31213 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Homo sapiens OX=9606 GN=SRD5A2 PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 2.3e-12
Identity = 49/168 (29.17%), Postives = 89/168 (52.98%), Query Frame = 0


Query: 106 HFFKRIFEVLFVHK---YSSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLK- 165
           H+F R F    +++   Y + ++L      + +  L    +IY      G+     D++ 
Sbjct: 90  HYFHRTFVYSLLNRGRPYPAILILRGTAFCTGNGVLQGYYLIYCAEYPDGW---YTDIRF 149

Query: 166 NIGIGCFVMGIIGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAF 225
           ++G+  F++G+  N +  Y+L + R  GE  Y+IP GGLF  V   ++L EII + G+A 
Sbjct: 150 SLGVFLFILGMGINIHSDYILRQLRKPGEISYRIPQGGLFTYVSGANFLGEIIEWIGYAL 209

Query: 226 ISQAIFPLCFATGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 270
            + ++  L FA  +  +L  R++   ++Y+  FE +P   KAL+PF+F
Sbjct: 210 ATWSLPALAFAFFSLCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF 254

[Close]

BLAST of MC09g1451 vs. NCBI nr
Match: XP_022150594.1 (very-long-chain enoyl-CoA reductase-like [Momordica charantia])

HSP 1 Score: 535 bits (1378), Expect = 1.02e-191
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0


Query: 4   MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 63
           MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES
Sbjct: 1   MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60

Query: 64  KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKYSSK 123
           KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKYSSK
Sbjct: 61  KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKYSSK 120

Query: 124 MVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHYLLS 183
           MVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHYLLS
Sbjct: 121 MVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHYLLS 180

Query: 184 RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRS 243
           RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRS
Sbjct: 181 RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRS 240

Query: 244 YATRKWYVSKFEHFPNHVKALLPFLF 269
           YATRKWYVSKFEHFPNHVKALLPFLF
Sbjct: 241 YATRKWYVSKFEHFPNHVKALLPFLF 266

[Close]

BLAST of MC09g1451 vs. NCBI nr
Match: XP_004135052.2 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 [Cucumis sativus] >KAE8648808.1 hypothetical protein Csa_008329 [Cucumis sativus])

HSP 1 Score: 452 bits (1164), Expect = 8.66e-159
Identity = 220/269 (81.78%), Postives = 245/269 (91.08%), Query Frame = 0


Query: 1   AAAMDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSS 60
           +AAMD+ SAFLFPPPPSTFVNVMTVVS+TA+AM+G SE+NGKHLQYSKFWNANGASK S+
Sbjct: 18  SAAMDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSA 77

Query: 61  EESKVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKY 120
           EESKVSSKYGMLLAYTPAFLAGAASFW FPS D R+ LLKSALT HFFKRIFEVLF+HKY
Sbjct: 78  EESKVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKY 137

Query: 121 SSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHY 180
           SSKMVL TA +ISLSYF+STAIMIYTQHLSQG P P IDLKNIG+G F++GIIGNFYHHY
Sbjct: 138 SSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHY 197

Query: 181 LLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLA 240
           LLS+TR +G+  YKIP GGLF  V+CPHYLFEII YFGFAFISQ ++PLCF+ GTA+YLA
Sbjct: 198 LLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLA 257

Query: 241 GRSYATRKWYVSKFEHFPNHVKALLPFLF 269
           GRSYATRKWY+SKFE FPN+VKALLPF+F
Sbjct: 258 GRSYATRKWYISKFEAFPNNVKALLPFVF 286

[Close]

BLAST of MC09g1451 vs. NCBI nr
Match: XP_022984951.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita maxima])

HSP 1 Score: 442 bits (1137), Expect = 5.46e-155
Identity = 217/266 (81.58%), Postives = 238/266 (89.47%), Query Frame = 0


Query: 4   MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 63
           M I SAFLFPPPPSTFVNVMTVVSSTAM M+G SE+NGKHLQYSKFWNAN + K+ SEE 
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 64  KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKYSSK 123
           K+SSKYGMLLAYTPAFLAGAASFWFFPSD+ RIL+LK+ALT HFFKRIFEVLF+HKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 124 MVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHYLLS 183
           MV DTA KIS SY LST IMIYTQHLSQG P P IDLKNIGI  F++GIIGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 184 RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRS 243
           +TR  GE  YKIP GGLF LVICPHYLFEII +FGFAFISQ ++PLCF+TGTA+YLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 244 YATRKWYVSKFEHFPNHVKALLPFLF 269
           YATRKWYVSKFE+FP+HVKALLPF+F
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of MC09g1451 vs. NCBI nr
Match: XP_022957108.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata])

HSP 1 Score: 442 bits (1137), Expect = 5.46e-155
Identity = 217/266 (81.58%), Postives = 238/266 (89.47%), Query Frame = 0


Query: 4   MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 63
           M I SAFLFPPPPSTFVNVMTVVSSTAM M+G SE+NGKHLQYSKFWNAN + K+ SEE 
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 64  KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKYSSK 123
           K+SSKYGMLLAYTPAFLAGAASFWFFPSD+ RIL+LK+ALT HFFKRIFEVLF+HKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 124 MVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHYLLS 183
           MV DTA KIS SY LST IMIYTQHLSQG P P IDLKNIGI  F++GIIGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 184 RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRS 243
           +TR  GE  YKIP GGLF LVICPHYLFEII +FGFAFISQ ++PLCF+TGTA+YLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 244 YATRKWYVSKFEHFPNHVKALLPFLF 269
           YATRKWYVSKFE+FP+HVKALLPF+F
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of MC09g1451 vs. NCBI nr
Match: XP_008446722.1 (PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis melo])

HSP 1 Score: 441 bits (1135), Expect = 1.58e-154
Identity = 216/269 (80.30%), Postives = 237/269 (88.10%), Query Frame = 0


Query: 1   AAAMDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSS 60
           + AMDI SAFLFPPPPSTFVNVMTVVSST++AM+G SE+ GKHLQYSKFWNAN  SK S+
Sbjct: 8   SVAMDILSAFLFPPPPSTFVNVMTVVSSTSLAMAGLSEIKGKHLQYSKFWNANSGSKLSA 67

Query: 61  EESKVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKY 120
           +ESK+SSKYGMLL YTPAFLAGAASFW FPS D R  LLKSALT HFFKRIFEVLF+HKY
Sbjct: 68  QESKLSSKYGMLLVYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKY 127

Query: 121 SSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHY 180
           SSKMVL TA +IS SYFLSTAIMIYTQHLSQG P P IDLKNIG+G F+ GIIGNFYHHY
Sbjct: 128 SSKMVLKTAIQISFSYFLSTAIMIYTQHLSQGLPEPPIDLKNIGVGLFLTGIIGNFYHHY 187

Query: 181 LLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLA 240
           LLS+TR + E  YKIP GGLF  VICPHYLFEII YFGFAFISQ ++PLCF+ GTA+YLA
Sbjct: 188 LLSQTRKQEETAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLA 247

Query: 241 GRSYATRKWYVSKFEHFPNHVKALLPFLF 269
           GRSYATRKWY+SKFE+FPNHVKALLPF+F
Sbjct: 248 GRSYATRKWYISKFENFPNHVKALLPFVF 276

[Close]

BLAST of MC09g1451 vs. ExPASy TrEMBL
Match: A0A6J1DAI6 (very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC111018692 PE=3 SV=1)

HSP 1 Score: 535 bits (1378), Expect = 4.93e-192
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0


Query: 4   MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 63
           MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES
Sbjct: 1   MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60

Query: 64  KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKYSSK 123
           KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKYSSK
Sbjct: 61  KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKYSSK 120

Query: 124 MVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHYLLS 183
           MVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHYLLS
Sbjct: 121 MVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHYLLS 180

Query: 184 RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRS 243
           RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRS
Sbjct: 181 RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRS 240

Query: 244 YATRKWYVSKFEHFPNHVKALLPFLF 269
           YATRKWYVSKFEHFPNHVKALLPFLF
Sbjct: 241 YATRKWYVSKFEHFPNHVKALLPFLF 266

[Close]

BLAST of MC09g1451 vs. ExPASy TrEMBL
Match: A0A6J1J6R3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111483043 PE=3 SV=1)

HSP 1 Score: 442 bits (1137), Expect = 2.64e-155
Identity = 217/266 (81.58%), Postives = 238/266 (89.47%), Query Frame = 0


Query: 4   MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 63
           M I SAFLFPPPPSTFVNVMTVVSSTAM M+G SE+NGKHLQYSKFWNAN + K+ SEE 
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 64  KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKYSSK 123
           K+SSKYGMLLAYTPAFLAGAASFWFFPSD+ RIL+LK+ALT HFFKRIFEVLF+HKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 124 MVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHYLLS 183
           MV DTA KIS SY LST IMIYTQHLSQG P P IDLKNIGI  F++GIIGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 184 RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRS 243
           +TR  GE  YKIP GGLF LVICPHYLFEII +FGFAFISQ ++PLCF+TGTA+YLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 244 YATRKWYVSKFEHFPNHVKALLPFLF 269
           YATRKWYVSKFE+FP+HVKALLPF+F
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of MC09g1451 vs. ExPASy TrEMBL
Match: A0A6J1GZA3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458588 PE=3 SV=1)

HSP 1 Score: 442 bits (1137), Expect = 2.64e-155
Identity = 217/266 (81.58%), Postives = 238/266 (89.47%), Query Frame = 0


Query: 4   MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 63
           M I SAFLFPPPPSTFVNVMTVVSSTAM M+G SE+NGKHLQYSKFWNAN + K+ SEE 
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 64  KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKYSSK 123
           K+SSKYGMLLAYTPAFLAGAASFWFFPSD+ RIL+LK+ALT HFFKRIFEVLF+HKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 124 MVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHYLLS 183
           MV DTA KIS SY LST IMIYTQHLSQG P P IDLKNIGI  F++GIIGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 184 RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRS 243
           +TR  GE  YKIP GGLF LVICPHYLFEII +FGFAFISQ ++PLCF+TGTA+YLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 244 YATRKWYVSKFEHFPNHVKALLPFLF 269
           YATRKWYVSKFE+FP+HVKALLPF+F
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of MC09g1451 vs. ExPASy TrEMBL
Match: A0A1S3BFS0 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucumis melo OX=3656 GN=LOC103489360 PE=3 SV=1)

HSP 1 Score: 441 bits (1135), Expect = 7.64e-155
Identity = 216/269 (80.30%), Postives = 237/269 (88.10%), Query Frame = 0


Query: 1   AAAMDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSS 60
           + AMDI SAFLFPPPPSTFVNVMTVVSST++AM+G SE+ GKHLQYSKFWNAN  SK S+
Sbjct: 8   SVAMDILSAFLFPPPPSTFVNVMTVVSSTSLAMAGLSEIKGKHLQYSKFWNANSGSKLSA 67

Query: 61  EESKVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKY 120
           +ESK+SSKYGMLL YTPAFLAGAASFW FPS D R  LLKSALT HFFKRIFEVLF+HKY
Sbjct: 68  QESKLSSKYGMLLVYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKY 127

Query: 121 SSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHY 180
           SSKMVL TA +IS SYFLSTAIMIYTQHLSQG P P IDLKNIG+G F+ GIIGNFYHHY
Sbjct: 128 SSKMVLKTAIQISFSYFLSTAIMIYTQHLSQGLPEPPIDLKNIGVGLFLTGIIGNFYHHY 187

Query: 181 LLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLA 240
           LLS+TR + E  YKIP GGLF  VICPHYLFEII YFGFAFISQ ++PLCF+ GTA+YLA
Sbjct: 188 LLSQTRKQEETAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLA 247

Query: 241 GRSYATRKWYVSKFEHFPNHVKALLPFLF 269
           GRSYATRKWY+SKFE+FPNHVKALLPF+F
Sbjct: 248 GRSYATRKWYISKFENFPNHVKALLPFVF 276

[Close]

BLAST of MC09g1451 vs. ExPASy TrEMBL
Match: A0A0A0KTC7 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G612880 PE=3 SV=1)

HSP 1 Score: 412 bits (1059), Expect = 2.15e-143
Identity = 204/269 (75.84%), Postives = 229/269 (85.13%), Query Frame = 0


Query: 1   AAAMDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSS 60
           +AAMD+ SAFLFPPPPSTFVNVMTVVS+TA+AM+G SE+NGKHLQYSKFWNANGASK S+
Sbjct: 18  SAAMDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSA 77

Query: 61  EESKVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKY 120
           EESKVSSKYGMLLAYTPAFLAGAASFW FPS D R+L                  F+HKY
Sbjct: 78  EESKVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVL------------------FIHKY 137

Query: 121 SSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHY 180
           SSKMVL TA +ISLSYF+STAIMIYTQHLSQG P P IDLKNIG+G F++GIIGNFYHHY
Sbjct: 138 SSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHY 197

Query: 181 LLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLA 240
           LLS+TR +G+  YKIP GGLF  V+CPHYLFEII YFGFAFISQ ++PLCF+ GTA+YLA
Sbjct: 198 LLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLA 257

Query: 241 GRSYATRKWYVSKFEHFPNHVKALLPFLF 269
           GRSYATRKWY+SKFE FPN+VKALLPF+F
Sbjct: 258 GRSYATRKWYISKFEAFPNNVKALLPFVF 268

[Close]

BLAST of MC09g1451 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 308.5 bits (789), Expect = 5.2e-84
Identity = 157/272 (57.72%), Postives = 202/272 (74.26%), Query Frame = 0


Query: 4   MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 63
           M++ ++F++PPPPS  +N M+VV   A+A  G+SE+ G HL+YSKF    G S SS +  
Sbjct: 1   MEMVTSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGNHLKYSKF----GVSSSSPQPQ 60

Query: 64  K-----VSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVH 123
           K     +SS+ GMLL YTPAFLA A+SF+  PSDD R LLLKSAL  HFFKR+FEVLF+H
Sbjct: 61  KERFGSISSRNGMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFEVLFIH 120

Query: 124 KYSSKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYH 183
           KYS  M +D+A  IS SYF STA+M+Y+Q+L+ G   PS D+K  G+  FV+GI+GN YH
Sbjct: 121 KYSGGMAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYH 180

Query: 184 HYLLSRTRGK-GEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTAL 243
           H LL++ R + G+KEYKIP GGLF ++ICPHYLFEI+V++ F  ISQ I+   FA GT L
Sbjct: 181 HVLLAKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTML 240

Query: 244 YLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 270
           YL GRSYATR WY+SKF+ FP H+KAL+PF+F
Sbjct: 241 YLIGRSYATRTWYLSKFDDFPKHIKALIPFVF 268

[Close]

BLAST of MC09g1451 vs. TAIR 10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 67.0 bits (162), Expect = 2.6e-11
Identity = 55/172 (31.98%), Postives = 78/172 (45.35%), Query Frame = 0


Query: 105 FHFFKRIFEVLFVHKYS-SKMVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNI 164
           FH+FKRI E  FVH++S +   +   F+    Y+   A + Y  +     P   + +K I
Sbjct: 140 FHYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYIAYYVNHPLYTPVSDLQMK-I 199

Query: 165 GIGCFVMGIIGNFYHHYLLSRTRG-KGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFI 224
           G G  ++  + NFY H LL   R   G   Y+IP G LF +V C +Y  EI  + GF   
Sbjct: 200 GFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNIA 259

Query: 225 SQAIFPLCFATGTALYLA----GRSYATRKWYVSK--FEHFPNHVKALLPFL 269
           +Q I    F    AL +     G+    RK +  K     +P     L PFL
Sbjct: 260 TQTIAGYVFLAVAALIMTNWALGKHSRLRKIFDGKDGKPKYPRRWVILPPFL 310

[Close]

BLAST of MC09g1451 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.3e-10
Identity = 38/108 (35.19%), Postives = 59/108 (54.63%), Query Frame = 0


Query: 163 IGIGCFVMGIIGNFYHHYLLSRTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFI 222
           IG+  F+ G+  N      L R + +    Y IP GG F LV CP+Y  E I + G+A +
Sbjct: 155 IGMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVM 214

Query: 223 SQAIFPLCFATGTALYLAGRSYATRKWYVSKF-EHFPNHVKALLPFLF 270
           + +   + F   T   L  R+ A+ KWY++KF E +P   KA++PF++
Sbjct: 215 TWSWAGIGFFLYTCSNLFPRARASHKWYIAKFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of MC09g1451 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 57.0 bits (136), Expect = 2.7e-08
Identity = 41/117 (35.04%), Postives = 57/117 (48.72%), Query Frame = 0


Query: 160 LKNIGIGCFVMGIIGNFYHHYLLSRTR--GKGEKEYKIPNGGLFCLVICPHYLFEIIVYF 219
           L+ IG   F+ G I     H +L   R      KEY IP G  F +V CPH+L EI++Y 
Sbjct: 207 LQWIGGAIFLWGWIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYL 266

Query: 220 GFAFISQ----AIFPLC-FATGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 270
           G    S     +I+ L  F      Y AG    T +WY+ KFE++P    A+ P ++
Sbjct: 267 GLLISSGGTDISIWLLFGFVAANLTYAAGE---THRWYLQKFENYPASRHAIFPHVY 320

[Close]

BLAST of MC09g1451 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 55.1 bits (131), Expect = 1.0e-07
Identity = 38/117 (32.48%), Postives = 54/117 (46.15%), Query Frame = 0


Query: 160 LKNIGIGCFVMGIIGNFYHHYLLS--RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYF 219
           L+ IG   F+ G I     H +L   R      KEY IP G  F +V  PH+L EI++Y 
Sbjct: 230 LQWIGGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYA 289

Query: 220 GFAFISQAI-----FPLCFATGTALYLAGRSYATRKWYVSKFEHFPNHVKALLPFLF 270
           G    S            F      Y AG    T +WY+ KFE++P +  A+ P+++
Sbjct: 290 GLLIASGGTDITIWLLFGFVAANLTYAAGE---THRWYLRKFENYPANRHAIFPYVY 343

The following BLAST results are available for this feature:

BLAST of MC09g1451 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q55C17	1.4e-14	33.53	Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn...	[more]
O18765	2.7e-13	37.04	3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Sus scrofa OX=9823 GN=SRD5A2 PE=2 SV=...	[more]
Q28892	1.0e-12	29.17	3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis OX=9541 GN=SRD5A2...	[more]
Q2QDF6	1.8e-12	30.00	Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1	[more]
P31213	2.3e-12	29.17	3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Homo sapiens OX=9606 GN=SRD5A2 PE=1 S...	[more]

BLAST of MC09g1451 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150594.1	1.02e-191	100.00	very-long-chain enoyl-CoA reductase-like [Momordica charantia]	[more]
XP_004135052.2	8.66e-159	81.78	3-oxo-5-alpha-steroid 4-dehydrogenase 2 [Cucumis sativus] >KAE8648808.1 hypothet...	[more]
XP_022984951.1	5.46e-155	81.58	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita maxima]	[more]
XP_022957108.1	5.46e-155	81.58	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata]	[more]
XP_008446722.1	1.58e-154	80.30	PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis melo]	[more]

BLAST of MC09g1451 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DAI6	4.93e-192	100.00	very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1J6R3	2.64e-155	81.58	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1GZA3	2.64e-155	81.58	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A1S3BFS0	7.64e-155	80.30	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A0A0KTC7	2.15e-143	75.84	S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6128...	[more]

BLAST of MC09g1451 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16010.1	5.2e-84	57.72	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT3G55360.1	2.6e-11	31.98	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	1.3e-10	35.19	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT1G72590.1	2.7e-08	35.04	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.1	1.0e-07	32.48	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 159..269 e-value: 3.8E-25 score: 88.6
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 164..244 score: 22.898247
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 9..268
None	No IPR available	PANTHER	PTHR10556:SF35	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEIN	coord: 9..268

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC09g1451.1	MC09g1451.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors